Дисертації з теми "Muscle apoptosis"
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Siu, Parco Ming-fai. "The role of apoptosis in muscle remodeling." Morgantown, W. Va. : [West Virginia University Libraries], 2005. https://etd.wvu.edu/etd/controller.jsp?moduleName=documentdata&jsp%5FetdId=4040.
Повний текст джерелаTitle from document title page. Document formatted into pages; contains xiv, 445 p. : ill. (some col.). Includes abstract. Includes bibliographical references.
Fellows, Adam Lee. "FOXO3a in vascular smooth muscle cell apoptosis." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/275687.
Повний текст джерелаSattiraju, Sandhya Ramani. "Apoptosis and necrosis drive muscle fiber loss in lipin1 deficient skeletal muscle." Wright State University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=wright1598626794423032.
Повний текст джерелаSmith, Sierra Marie. "FoxO1 Induces Apoptosis in Skeletal Myotubes." University of Toledo / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1270334870.
Повний текст джерелаPESSINA, PATRIZIA. "Necdin enhances muscle reconstitution of dystrophic muscle by mesoangioblast cells." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2009. http://hdl.handle.net/10281/7594.
Повний текст джерелаKoutsouki, Evgenia. "The role of cadherins in vascular smooth muscle cell apoptosis." Thesis, University of Bristol, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.420910.
Повний текст джерелаHarford, Terri J. "Regulation of Apoptosis by the Muscle Regulatory Transcription Factor MyoD." Cleveland State University / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=csu1265812933.
Повний текст джерелаStorbeck, Christopher J. "Effects of the myotonic dystrophy mutation in muscle differentiation and apoptosis." Thesis, University of Ottawa (Canada), 2002. http://hdl.handle.net/10393/6194.
Повний текст джерелаLeskinen, Markus. "Mast cell-mediated apoptosis of smooth muscle cells and endothelial cells." Helsinki : University of Helsinki, 2003. http://ethesis.helsinki.fi/julkaisut/laa/kliin/vk/leskinen/.
Повний текст джерелаPistilli, Emidio E. "The extrinsic apoptotic pathway in aged skeletal muscle roles of tumor necrosis factor-[alpha] and interleukin-15 /." Morgantown, W. Va. : [West Virginia University Libraries], 2006. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=4912.
Повний текст джерелаTitle from document title page. Document formatted into pages; contains x, 189 p. : ill. (some col.). Includes abstract. Includes bibliographical references.
Slimani, Lamia. "Mécanismes impliqués dans l'atrophie et la récupération musculaire après immobilisation chez le rat. : Rôle des altérations de la matrice extracellulaire." Thesis, Clermont-Ferrand 1, 2012. http://www.theses.fr/2012CLF1MM21/document.
Повний текст джерелаSkeletal muscle is the main reservoir of body amino acids. Thus, muscle atrophy induced by immobilization can lead to a weakening and to a lengthening of recovery periods, leading to elevated healthcare costs. Surprisingly, a worsening of tibialis anterior (TA) muscle atrophy prevailed after cast removal and thus delayed recovery. The aim of my Ph.D was to understand mechanisms underlying the worsening of TA atrophy during early recovery by studying i) the muscle structure and phenotype, ii) the composition of the extracellular matrix (ECM), iii) proteolysis and apoptosis, and iv) the signaling pathways via integrins. Rats were subjected to hindlimb casting for 8 days of one hindllimb, the other leg served as control, and then were allowed to recover for 10 days. The worsening of TA atrophy appeared immediately after cast removal and correlated with i) a decrease in fiber crosssection area associated to fiber deformation, ii) a redistribution of myosin heavy chain isoforms, iii) an increase in apoptosis localized in the connective tissue, iv) a thickening of the endomysium during remobilization, v) some adaptations in collagen remodeling processes, and vi) a pronounced and sustained activation of the ubiquitin-proteasome proteolytic system (UPS) and of the apoptosome. We also showed an increase in the remobilized TA of mRNA levels vii) of tenascin-C and Sparc immediately after cast removal, and viii) of some autophagy markers, when atrophy stabilized. Finally, we showed an elevation of mRNA levels encoding ix) myogenic factors, and x) transmembrane integrins and their partners during TA immobilization and after cast removal. In conclusion, my Ph.D project showed that the worsening of the TA atrophy occurred early after cast removal, was associated with a significant remodeling of the structure and composition of the ECM and of the phenotype of muscle fibers, and may result from pronounced and sustained increase in the UPS and apoptosis. This work suggests that changes in the matricellular matrix molecules during remobilization could influence integrin-dependent signaling and muscle regeneration
Maltais, Jean-Sébastien. "RAGE comme nouvelle cible thérapeutique prévenant le stress du réticulum endoplasmique et l’apoptose des cellules du muscle lisse vasculaire associés avec le diabète." Thèse, Université de Sherbrooke, 2016. http://hdl.handle.net/11143/8804.
Повний текст джерелаAbstract : Cardiovascular diseases represent, to a large extent, the first cause of morbidity and mortality among people with diabetes. RAGE activation by advanced glycation end products (AGE) generated in hyperglycemic conditions is associated to a multitude of vascular diabetic complications, in particular by a signaling promoting chronic inflammation as well as death of cells forming tissues and organs exposed to AGE. Overexpression of RAGE in smooth muscle cells of vulnerable atheromatous plaques suggests the receptor could contribute to heart attacks and strokes. Therefore, we hypothesize that RAGE activation in smooth muscle cells is involved in apoptosis. To verify this hypothesis, we first designed a new label-free assay based of surface plasmon resonance (SPR) to measure apoptosis of a cell monolayer in real-time and to characterize precisely the kinetic parameters of the initiation and execution phases. This assay showed that RAGE activation induces apoptosis of more than 75.6% of smooth muscle cells stimulated with CML-HSA for 20 hours. Moreover, we noticed that RAGE activation generated strong endoplasmic reticular stress, indicated by the formation of a great number of stress granules as well as the increased expression of stress marker HuR and caspase-9, two important regulators of reticular stress-induced apoptosis. In order, to assess the potential of an antagonist to block RAGE activation, we then synthesized the iRAGE peptide whose sequence is derived from a binding site of CML-HSA that has the particularity of owning numerous negative charges at physiological pH. Pretreatment with iRAGE was successful to prevent activation of NF-κB, induction of apoptosis and generation of endoplasmic reticular stress. We suggest a model by which iRAGE inhibits RAGE signaling by hindering the binding of multimeric ligands and by stabilizing the receptors in a monomer state. Ultimately, the synthesis of a RAGE antagonist usable in clinic could constitute a major progress in the prevention of vascular complications and in the quality of life of people with diabetes.
Mitchell, Lylieth Paula-Ann. "Vascular endothelial and smooth muscle cell apoptosis in vivo and in vitro." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq28795.pdf.
Повний текст джерелаLoro, Emanuele Loro. "Normal myogenesis and increased apoptosis in myotonic dystrophy type-1 muscle cells." Doctoral thesis, Università degli studi di Padova, 2010. http://hdl.handle.net/11577/3423200.
Повний текст джерелаLa distrofia miotonica di tipo 1 (DM1) è causata dall'espansione (CTG)n nella regione trascritta ma non tradotta al 3' del gene DMPK. I trascritti mutati sono trattenuti in foci nucleari, i quali sequestrano diverse proteine leganti RNA spesso alterandone le funzioni (i.e. regolazione dello splicing). A livello del muscolo, i meccanismi patogenetici che portano a miotonia, debolezza e perdita di massa dei muscoli distali, non sono ad oggi chiari. Otto linee di mioblasti primari umani, ottenuti da biopsie di pazienti affetti da DM1 nelle forme adulta e congenita (range di espansione tra 90 e 1800 CTG), sono state differenziate ed innervate con successo, ottenendo miotubi in grado i contrarre. L'analisi morfologica e la quantificazione di diversi marker di miogenesi mediante RT-PCR e Western blotting, hanno indicato che il diferenziamento in vitro dei mioblasti primari DM1 è indistinguibile da quello ottenuto con mioblasti di controllo. In ciascuna linea DM1 è stata confermata l'espansione (CTG)n mediante long-PCR ed ibridizzazione in situ. Inoltre, nei miotubi DM1 è stato rilevata l'alterazione dello splicing del recettore per l'insulina e di MBNL1, caratteristica del fenotipo DM1. A 15 giorni di differenziamento, una considerevole perdita di miotubi DM1 ha suggerito l'attivazione di pathways catabolici, come confermato dalla presenza di marker di apoptosi (taglio proteolitico della caspasi 3, rilascio di citocromo c dai mitocondri, frammentazione della cromatina) e di autofagia (aumento dei livelli di LC3 lipidato e di P62). Il trattamento con l'inibitore delle caspasi Z-VAD si è dimostrato efficace nell'attenuare la riduzione del numero di mionuclei e del calibro medio dei miotubi a 15 giorni di differenziamento. Proponiamo quindi che la compromissione muscolare tipica della DM1 sia dovuta, più che ad un'alterata miogenesi, a problemi nei meccanismi di mantenimento/rigenerazione, che si esplicano attraverso la prematura attivazione di apoptosi e/o autofagia
Gadde, Murali K. "Effects of aging on regulators of muscle apoptosis in the female F344BN rat." [Huntington, WV : Marshall University Libraries], 2009. http://www.marshall.edu/etd/descript.asp?ref=998.
Повний текст джерелаEnwere, Emeka K. "Regulation of Skeletal Muscle Formation and Regeneration by the Cellular Inhibitor of Apoptosis 1 (cIAP1) Protein." Thèse, Université d'Ottawa / University of Ottawa, 2011. http://hdl.handle.net/10393/20048.
Повний текст джерелаHazelett, Dennis J. "Gene expression during the segment-specific death of a muscle during insect metamorphosis /." view abstract or download file of text, 2005. http://wwwlib.umi.com/cr/uoregon/fullcit?p3164079.
Повний текст джерелаTypescript. Includes vita and abstract. Includes bibliographical references (leaves 118-133). Also available for download via the World Wide Web; free to University of Oregon users.
Granville, David James. "Biochemical mechanisms of endothelial and smooth muscle cell apoptosis induced by photodynamic therapy." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/NQ61099.pdf.
Повний текст джерелаSchaub, Friedemann. "Fas/FADD-induced pro-inflammatory response in vascular smooth muscle cells /." Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/6345.
Повний текст джерелаWaltmann, Meaghan D. "Apolipoprotein E receptor 2 deficiency alters smooth muscle cell and macrophage characteristics to promote atherosclerotic lesion necrosis." University of Cincinnati / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1384851103.
Повний текст джерелаZee, Michele Chi-Wai. "Steroid hormones and cell death : analysis of motorneuron and muscle fates during insect metamorphosis /." view abstract or download file of text, 2004. http://wwwlib.umi.com/cr/uoregon/fullcit?p3136456.
Повний текст джерелаTypescript. Includes vita and abstract. Includes bibliographical references (leaves 99-113). Also available for download via the World Wide Web; free to University of Oregon users.
Dishmon, Monja. "cFLIP regulates Fas-induced apoptosis and pro-inflammatory gene expression in human vascular smooth muscle cells /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/6341.
Повний текст джерелаSingh, François. "Skeletal muscle toxicity and statins : role of mitochondrial adaptations." Thesis, Strasbourg, 2016. http://www.theses.fr/2016STRAJ050/document.
Повний текст джерелаAlthough statins are the most prescribed class of lipid-lowering agents, adverse muscular toxicity has been reported, which can lead to the appearance of a myopathy. In the first part, we showed in Humans and animals that statins inhibit directly the mitochondrial respiratory chain, and induce the production of reactive oxygen species (ROS), that trigger apoptotic pathways in glycolytic skeletal muscles, whereas oxidative muscles are not impaired. We then showed in vitro that reductive stress can provoke mitochondrial oxidation, that could lead to an activation of mitochondrial biogenesis pathways. Moreover, the consequent increase in mitochondrial content enabled to protect cells against statin-induced apoptosis. Finally, we showed in vivo that the induction of mitochondrial biogenesis is necessary for statin tolerance in oxidative skeletal muscles. In conclusion, mitochondrial phenotype, both quantitatively and qualitatively, seems to be a key factor in the appearance of statin myopathy
Tan, Yu Yin Nicole Medical Sciences Faculty of Medicine UNSW. "Gene expression during activation of smooth muscle cells." Publisher:University of New South Wales. Medical Sciences, 2009. http://handle.unsw.edu.au/1959.4/43615.
Повний текст джерелаD'Antoni, Michelle. "The effect of decorin and biglycan on human airway smooth muscle cell proliferation, apoptosis and attachment." Thesis, McGill University, 2011. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=103624.
Повний текст джерелаLes changements de composition de la matrice extracellulaire (MEC) ainsi que l'augmentation de la masse du muscle lisse des voies aériennes sont des caractéristiques du remodelage des voies aériennes qui contribuent à la physiopathologie de l'asthme. Les modifications de la MEC comprennent une augmentation du dépôt de collagène et un changement du niveau des protéoglycanes (PGs). Le dépôt de biglycane, un petit PG riche en leucine, est généralement plus élevé. Les changements d'expression de la décorine, un autre petit PG riche en leucine, sont, par contre, plus variables. L'interaction entre la cellule et la MEC qui l'entoure, déclenche de nombreuses réponses cellulaires, telles que la survie, la prolifération et la différenciation. Étant donné que la décorine et le biglycane peuvent influencer le comportement de différents types de cellules, nous avons émis l'hypothèse que ces molécules auraient aussi un effet sur la fonction des cellules musculaires lisses des voies aériennes. Nous avons cherché à déterminer les effets de la décorine et du biglycane sur la prolifération, l'apoptose et l'attachement des cellules musculaires lisses des voies aériennes. Nous avons aussi exploré les mécanismes putatifs qui seraient responsables de ces effets en utilisant le collagène de type I sera comme traitement de référence. La culture des cellules sur une matrice de décorine a provoqué une diminution du nombre cellules musculaires lisses des voies aériennes due à une diminution de la prolifération et à une augmentation de l'apoptose. La culture sur le biglycane a causé initialement une diminution du nombre de cellules, mais cette baise n'a pas été maintenue. Le collagène a provoqué une augmentation significative du nombre de cellules. Ni le biglycane ni le collagène n'ont induit des changements au niveau de la prolifération ou de l'apoptose. Des expériences ultérieures ont démontré que l'ancrage des cellules musculaires lisses des voies aériennes sur des matrices de décorine et de biglycane était anormal, ainsi que la formation d'adhésions focales et l'organisation du cytosquelette, en comparaison avec les cellules ensemencées sur du collagène ou du plastique. De plus, l'augmentation d'expression protéique de la sous-unité alpha2 d'intégrine a également été observée. Pour évaluer l'activation des intégrines, le niveau de protéine, ainsi que l'activation de la kinase des adhésions focales (FAK) ont été mesurés. En présence de décorine, l'expression protéique de FAK était significativement diminuée comparativement aux niveaux produits par les cellules ensemencées sur du plastique ou du collagène bien que le niveau d'activation était inchangé. Ces études démontrent que les PGs ont des effets distincts sur la croissance des cellules musculaires lisses des voies aériennes: la décorine a réduit la prolifération et a augmenté l'apoptose des cellules, tandis que le biglycane n'a pas modifié ces paramètres. Les adhésions cellules-matrice des cellules musculaires lisses des voies aériennes ensemencées sur la décorine ou le biglycane étaient irrégulières par rapport aux cellules sur le collagène de type I ou le plastique. La diminution de la quantité de décorine dans la paroi des voies aériennes observée dans les cas d'asthme fatal pourrait entraîner une hyperplasie plus prononcée des cellules musculaires lisses des voies aériennes. Ceci suggère que la présence de la décorine pourrait jouer un rôle protectif dans l'asthme, en modulant l'augmentation de la masse du muscle lisse des voies aériennes.
Woolard, Jeanette. "Mechanisms of regression of vascular structure during antihypertensive therapy, hypoxia-induced apoptosis in vascular smooth muscle cells." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape9/PQDD_0002/MQ42706.pdf.
Повний текст джерелаMaddali, Kamala Kalyani. "A mandatory requirement of PKC-[delta] in testosterone regulated coronary smooth muscle cell differentiation, proliferation and apoptosis /." Free to MU Campus, others may purchase, 2005. http://proquest.umi.com/pqdweb?did=1232392431&sid=1&Fmt=2&clientId=45247&RQT=309&VName=PQD.
Повний текст джерелаWang, Yenfeng. "The role of mast cells in foam cell formation, growth inhibition, and apoptosis of smooth muscle cells." Helsinki : University of Helsinki, 1999. http://ethesis.helsinki.fi/julkaisut/mat/bioti/vk/wang/.
Повний текст джерелаLima, Tãnia Marisa da Costa. "Molecular and functional changes in cardiac and skeletal muscle in HFpEF remodelling and reverse remodelling." Master's thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/22238.
Повний текст джерелаA insuficiência cardíaca (IC) com fração de ejeção preservada (ICFEp) é uma síndrome com uma etiologia muito diversificada, cuja disfunção metabólica tem sido apontada como um importante mecanismo associado à sua severidade. A remodelagem do miocárdio, resulta de uma agressão ao coração que pode ser direta (isquemia, estenose aórtica, etc) ou indireta (diabetes, disfunção renal, etc). Quando esta agressão é atenuada, por tratamento farmacológico ou cirúrgico, o coração sofre uma remodelagem reversa (RR) e o miocárdio retoma à sua estrutura e função normais. Conhecer os mecanismos subjacentes ao padrão de remodelagem e RR do miocárdio irá certamente potenciar novas oportunidades de tratamento da ICFEp. Por ser uma síndrome multisistémica, os doentes com ICFEp apresentam frequentemente sinais e sintomas extra-cardíacos característicos do diagnóstico desta patologia, como é o caso da intolerância ao esforço. Assim este trabalho teve como objetivos implementar e caracterizar um modelo animal de ICFEp, bem como avaliar as alterações estruturais, funcionais e moleculares que ocorrem ao nível do músculo cardíaco e esquelético na remodelagem e RR. Os nossos resultados mostram que a implementação de um modelo animal que mimetiza o fenótipo de ICFEp foi bem-sucedida. De facto, os animais banding apresentaram uma marcada hipertrofia do ventrículo esquerdo (VE), disfunção diastólica com rigidez do miocárdio, alterações na regulação do cálcio e aumento do stress oxidativo. Observaram-se ainda alterações que sugerem um aumento da biogénese e da fissão mitocondrial bem como um aumento dos transportadores de glucose. Apesar do aumento da expressão da proteína desacopladora 1 (UCP-1), funcionalmente, as mitocôndrias apresentaram uma melhoria da sua função. A redução da performance física dos animais banding foi acompanhada de alterações estruturais ao nível do músculo-esquelético, assim como de uma alteração dos transportadores dos substratos metabólicos. Curiosamente, nos animais debanding, apesar da recuperação funcional, morfologicamente o miocárdio não normalizou totalmente. Adicionalmente, observou-se um aumento dos transportadores de ácidos gordos, acompanhado por uma diminuição do stress oxidativo e da apoptose no VE. Além disso, apesar da melhoria metabólica, as mitocôndrias do VE dos animais debanding mantém-se menores. Relativamente à capacidade aeróbica dos animais, observou-se uma melhoria após o debanding acompanhada por uma reversão da atrofia e a fibrose das fibras musculares, assim como da oxidação dos ácidos gordos. Este trabalho mostra evidências do envolvimento mitocondrial e metabólico na progressão da ICFEp, ao nível dos músculo-esquelético e cardíaco.
Heart failure (HF) with preserved ejection fraction (HFpEF) is a complex syndrome with a diverse aetiology in which the metabolic dysfunction has been pointed out as an important mechanism that underlies the disease severity. Myocardial remodelling results from cardiac injury that can be direct (ischemia, aortic stenosis, etc) or indirect (diabetes, renal dysfunction, etc). When the deleterious stimulus is attenuated by pharmacological or surgical treatment, the heart enrols in a process called reverse remodelling (RR), and myocardial structure and function returns to normal. The knowledge of the molecular mechanism that underlie the RR process could represent an opportunity to develop novel therapeutic approaches and thus improve the treatment of HFpEF patients. As being a multi-systemic syndrome, HFpEF presents several extra-cardiac signals and symptoms typical of its diagnosis, such as effort intolerance. Thus, the aims of this work was to implement and characterize an animal model of cardiac remodelling and reverse remodelling of HFpEF and thus characterize structurally, functionally and molecularly the changes that occurs at the myocardium and at the skeletal muscle. Our results showed that we successfully implemented an animal model of HFpEF that presents an LV hypertrophic and increased stiffness. Additionally to LV diastolic dysfunction (DD) we also observed abnormalities on calcium and oxidative stress. In banding rats we denoted an increase of peroxisome proliferator-activated receptor-gamma coactivator alpha (PGC-1α) and downregulation of mitofusin (MNF1,2) as well as an augment of glucose transporters. Despite de increase of uncoupled protein 1 (UCP-1) expression, functionally we denoted an improvement of mitochondria respiration and membrane potential. The physical performance of banding animals was impaired and accomplished by structural changes at skeletal muscle level as well as at metabolic substrate transporters. Curiously, after afterload relief despite the functionally recovery, morphologically the myocardial reverse remodelling was incomplete. Moreover, regardless the metabolic transporters reversion the mitochondria continue smaller. After overload relief the rats showed an improvement on aerobic capacity as well as a reversion on skeletal muscle atrophy, fibrosis and an upregulation of FA oxidation. The present study shows clearly the involvement of mitochondria and metabolism on myocardial and skeletal muscle remodelling and RR.
Unlu, Sebnem. "Role of isoprenylation in the control of cell proliferation and apoptosis in human vascular smooth muscle cells in culture." Thesis, Imperial College London, 2000. http://hdl.handle.net/10044/1/8577.
Повний текст джерелаSoutoudeh, Mohammad. "Use of a newly designed equi-biaxial stretch device to study stretch-induced apoptosis of vascular smooth muscle cells /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1998. http://wwwlib.umi.com/cr/ucsd/fullcit?p9913164.
Повний текст джерелаMarchildon, François. "The Role of CCAAT/Enhancer Binding Protein Beta (C/EBPβ) in Skeletal Muscle Satellite Cells after Injury and in Cancer Cachexia". Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/33373.
Повний текст джерелаBarrett, Brianna L. "MOLECULAR DISTINCTIONS REGULATING THE TEMPORAL EXPRESSION OF THE MYOD-RESPONSIVE GENES PUMA (RESPONSIBLE FOR APOPTOSIS) AND MYOGENIN (RESPONSIBLE FOR DIFFERENTIATION)." Cleveland State University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=csu1557264097566887.
Повний текст джерелаScholtes, Charlotte. "Étude des liens entre les acteurs de la dynamique mitochondriale et l'apoptose dans la dégénérescence musculaire dystrophinedépendante chez Caenorhabditis elegans." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1001.
Повний текст джерелаMitochondrial shape is continually changing thanks to the combined actions of fission and fusion events making the mitochondrial network very dynamic. The mitochondrial fission and fusion processes are finely regulated by GTPases of the family of dynamins that are well conserved between species. In C. elegans, fission is regulated by DRP-1, fusion of the inner membrane by EAT-3, homologue of OPA1, and fusion of the outer membrane by FZO-1, homologue of MFN1. In the muscle cells of wild nematode, tubular and circular mitochondria are in equal proportions and organized along the sarcomere. However, during dystrophin-dependent muscle degeneration, fragmentation of the mitochondrial network in muscle cells occurs. But the role of the actors of mitochondrial dynamics in the molecular mechanisms leading to dystrophin-dependent muscle degeneration is still misunderstood. We found that: (i) dystrophin-dependent muscle degeneration was accompanied by a drastic increase in mitochondrial fragmentation that can be saved by genetic manipulation of mitochondrial dynamics (ii) loss of function of the fission gene drp-1 or overexpression of the eat-3 and fzo-1 fusion genes causes a reduction in muscle degeneration and improved mobility of dystrophic mutants (iii) DRP-1 functions in apoptosis and other are important for the death of dystrophin-deficient muscle cells (iv) The involvement of DRP-1 in apoptosis is also important for age-dépendant muscle degeneration. In conclusion, our results point toward a mechanism involving mitochondrial dynamics to impact muscle degeneration via apoptosis in Caenorhabditis elegans
Foster, Cerrone R., Laura L. Daniel, Christopher R. Daniels, Suman Dalal, Mahipal Singh, and Krishna Singh. "Deficiency of Ataxia Telangiectasia Mutated Kinase Modulates Cardiac Remodeling Following Myocardial Infarction: Involvement in Fibrosis and Apoptosis." Digital Commons @ East Tennessee State University, 2013. https://dc.etsu.edu/etsu-works/8570.
Повний текст джерелаAndrianjafiniony, Tina. "Atrophie musculaire et récupération : homéostasie calcique, stress oxydant, apoptose et protéolyse musculaire." Thesis, Lyon 1, 2010. http://www.theses.fr/2010LYO10171.
Повний текст джерелаExposure to hypokinesia induces a functional and phenotypic atrophy of skeletal muscle. Several types ofmechanisms have been suggested to contribute to this plasticity phenomenon, including changes inintracellular calcium handling and in production of reactive oxygen species which, together withinflammatory processes, would activate muscle apoptosis and proteolysis pathways. The present workspecifically focussed on these mechanisms within the framework of a model of hypokinesia-inducedatrophy and of recovery from this atrophy. Measurements on isolated muscle cells revealed that the rateof myoplasmic calcium extrusion was considerably reduced in atrophied muscle fibres. This effect whichappears to be, at least in part, related to an altered mitochondrial contribution, may play a pivotal role inthe activation of calcium-dependent proteolysis pathways. We then studied the time course of changes inoxidative stress as well as in the expression level of several cytokines and proteins specifically involvedin proteolysis and caspase-dependent apoptotic pathways, along the course of recovery from atrophy. Ourresults demonstrate that, at early stages (1-5 days) of recovery, muscle re-growth is mediated via themodulation of mitochondrial-driven apoptosis and muscle proteolysis. In contrast, oxidative stress and theTNF-a related apoptotic pathway remain activated until late stages (14 days) of recovery, at a time whenmuscle mass has already recovered
Silva, Ramiro Hesiquio. "Avaliação de proteção celular a isquemia de retalhos musculares com soluções preservadoras de tecidos em modelo de ratos." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/5/5158/tde-29092009-165603/.
Повний текст джерелаThe free flaps transfer and reimplantation of tissue has in common the exposure of the tissue to ischemia and different time of reperfusion which are important for the determination of the extent of the cellular injury, being sometimes irreversible. In the pre and trans-surgical procedure the control of the ischemic period is difficult. Although efforts are made to prevent and decrease cellular changes using preservative solution, such as University of Wisconsin (UW) used in routine transplant of organs. The availability and high cost of this solution some time is one problem. The present study was made in a rat model that we have been reported in others works, we proposed the utilization of a preservation solution that we called Plastic Surgery-USP solution (PS-USP); witch can be easily prepared with accessible and low cost drugs. The efficiency of this PS-USP solution was compared to UW; our result showed that there is not significative difference in the protective effects of the PS-USP and UW solutions; both solutions were efficient considering cellular protection to ischemia/reperfusion injury, decreasing the risks of flap lost, with low cost and easy disposition
Lejmi, Mrad Rim. "Genetic and Pharmacologic Inhibition of Cellular Inhibitor of Apoptosis 1 (cIAP1) Protein Expression Protects Against Denervation-Induced Skeletal Muscle Atrophy In Vivo." Thesis, Université d'Ottawa / University of Ottawa, 2016. http://hdl.handle.net/10393/34260.
Повний текст джерелаSchulz, Cathrin. "Tumour-selective apoptosis : identification of NMHCIIa as novel death receptor interactor regulating the response to TRAIL." Phd thesis, Université de Strasbourg, 2012. http://tel.archives-ouvertes.fr/tel-01069133.
Повний текст джерелаLing, Shanhong. "Effects of estrogens on the vasculature in vitro cell culture studies." Monash University, Dept. of Medicine, 2003. http://arrow.monash.edu.au/hdl/1959.1/9345.
Повний текст джерелаWismann, Jennifer Willoughby Darryn Scott. "Effects of 28 days of protein and amino acid supplementation and ankle immobilization on gastrocnemius muscle mass and strength and atrophy- and apoptosis-related gene expression in males." Waco, Tex. : Baylor University, 2008. http://hdl.handle.net/2104/5181.
Повний текст джерелаAlharthi, Sameer E. M. "The effect of mitogen-activated protein kinase phosphatase-2 (MKP-2) over-expression via infection with Adv.MKP-2 on human endothelial cell apoptosis and vascular smooth muscle cell proliferation." Thesis, University of Strathclyde, 2010. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=14479.
Повний текст джерелаYadav, Prashant. "Understanding the Role of Phosphoinositide 3-Kinase and its Function as a Driving Force behind the ER Stress Response in Fibrostenotic Crohn’s Disease-affected Ileal Smooth Muscle Cells." VCU Scholars Compass, 2018. https://scholarscompass.vcu.edu/etd/5511.
Повний текст джерелаArnold, Ludovic Chazaud Bénédicte. "Rôle des macrophages dans la régénération du muscle strié squelettique." Créteil : Université de Paris-Val-de-Marne, 2007. http://doxa.scd.univ-paris12.fr:8080/theses-npd/th0357710.pdf.
Повний текст джерелаKiskin, Fedir. "Developing an induced pluripotent stem cell model of pulmonary arterial hypertension to understand the contribution of BMPR2 mutations to disease-associated phenotypes in smooth muscle cells." Thesis, University of Cambridge, 2019. https://www.repository.cam.ac.uk/handle/1810/285324.
Повний текст джерелаHe, Wei. "Elucidating the Molecular and Cellular Mechanism Underlying Cancer Cachexia." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1385054981.
Повний текст джерелаMoraes, Luis Henrique Rapucci. "A reinervação do músculo extensor longo dos dedos (EDL) de ratos (rattus norvegicus) seria influenciada pelo uso do laser de baixa potência e do tecido adiposo na técnica de tubulização?" Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/25/25142/tde-18082010-090713/.
Повний текст джерелаThe peripheral nerves injuries with morphofunctional alterations, have great clinical importance because could prejudice the function, committing the sensibility and/or the motricity of target organ. When nerve is damage, the individual becomes disabled to realize yours activities, either professional or personal, in the post accident periods, this situation aggravates each more, therefore initiate a trajectory of suffering and distressing despite of the kind of assistance that this person receives, in view of your emotional fragility and your moral discouragement that pass to be victim. In attempt to repair severe peripheral nerves lesions, many techniques had been used, but some present functional prejudices to other area of bodies, for example when other autologous nerve graft it is used. Considering that, vein graft had demonstrated good results in regenerative nerve fibers capacity, and the vein are found in abundance in many locals of chirurgic access, it thought in verify if the adipose tissue and low power laser could alter the reinnervation results, by tubulization technique, in fast twitch muscle (EDL). For this, was used 84 rats (Rattus norvegicus) wistar, male, divided in 12 groups (eight experimental and four controls). In the experimental groups (EG) was used tubulization by vein combined / or not with adipose tissue (EGV and EGVA, receptively), with or without laser treatment (EGVL and EGVAL, respectively). The controls groups (CG) was called of positives (CGP) when the animals did not subject to transaction nerve, and negatives (CGN) when the sciatic nerve was transaction in this animals. All groups had the animals scarified in two periods, 45 and 150 days post experiments beginning. The recuperation was notified by means of muscle innervated analysis (EDL), comparing with the respective controls groups. Microscope techniques, Immunofluorescence for (MyoD and Miogenin), apoptosis by (Tunel assay), morphometrics and sciatic functional analysis, were employed in this investigation. The results showed that in the 45 days post-dennervation, the data of experimental groups was nearest of negative control group (transaction sciatic nerve), but in the 150 days they was nearest to the positive control group. Based on this, could be conclude that the use of adipose tissue and low power laser used in the tubulization technique by vein in the sciatic nerve interfere in the recuperation of EDL muscle dennervated.
Netherland, Courtney Denise. "Role of Type 2 Cannabinoid Receptor (CB2) in Atherosclerosis." Digital Commons @ East Tennessee State University, 2011. https://dc.etsu.edu/etd/1392.
Повний текст джерелаNeves, Dalvaci da Cunha Lira. "Quantificação das células estreladas ativadas / miofibroblastos e análise da apoptose das células do fígado durante a terapia celular na fibrose hepática em ratos." Universidade do Estado do Rio de Janeiro, 2011. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=3730.
Повний текст джерелаA fibrose hepática é o resultado de uma resposta cicatrizante frente a repetidas lesões no fígado, e é caracterizada pelo acúmulo excessivo de proteínas da matriz extracelular (MEC) no parênquima hepático, incluindo colágeno, fibronectina, elastina, laminina e proteoglicanos, com a participação de diferentes populações celulares do fígado. As principais células responsáveis pela síntese de proteínas da MEC na fibrose hepática são as células estreladas hepáticas ativadas e os miofibroblastos, que surgem após estímulo inflamatório e são caracterizadas pela expressão de alfa-actina de músculo liso (α-SMA). Sabe-se que durante a progressão da fibrose hepática, ocorre a morte de hepatócitos e sua substituição por células fibrogênicas α-SMA+. A apoptose dessas células fibrogênicas é de grande relevância para a regressão da fibrose e regeneração hepática. Nos últimos anos, a terapia com células tronco de medula óssea tem sido utilizada para estimular a regeneração hepática em diferentes modelos experimentais e protocolos clínicos. A fração mononuclear da medula óssea adulta possui duas populações de células-tronco importantes no tratamento de diversas doenças hepáticas: células-tronco hematopoiéticas e células-tronco mesenquimais. O objetivo deste estudo foi analisar a expressão de α-SMA e o processo de apoptose de células hepáticas durante a fibrose hepática induzida por ligadura do ducto biliar (LDB) e após o transplante de células mononucleares de medula óssea (CMMO). Os fígados foram coletados de ratos dos seguintes grupos: normal, 14 dias de LDB, 21 dias de LDB e animais que receberam CMMO após 14 dias de LDB, e foram analisados após 7 dias (totalizando 21 dias de LDB). Para quantificar a expressão de α-SMA por células fibrogênicas nos grupos experimentais, foi realizada imunoperoxidase para α-SMA, seguida de morfometria no programa Image Pro Plus. Para analisar a apoptose nas células hepáticas, foi realizada imunoperoxidase e Western Blotting (WB) para caspase-3 (proteína apoptótica) e imunofluorescência com dupla-marcação para caspase-3 e α-SMA, seguida de observação em microscópio confocal. Os resultados da quantificação de α-SMA por morfometria mostraram que a expressão de α-SMA aumentou significativamente 14 e 21 dias após a LDB. Entretanto, essa expressão diminuiu significativamente no grupo tratado com CMMO, que apresentou parênquima hepático mais preservado em relação ao grupo com 21 dias de LDB. Os resultados de imunoperoxidase, WB e microscopia confocal para expressão de caspase-3 demonstraram que essa proteína diminuiu nos animais fibróticos com 14 e 21 dias de LDB com relação ao grupo normal, e estava significativamente elevada no grupo tratado com CMMO. A análise por microscopia confocal demonstrou que algumas células coexpressaram α-SMA e caspase-3 nos animais tratados com CMMO, sugerindo a morte de células fibrogênicas e remodelamento do parênquima hepático.
Hepatic fibrosis is the result of a scarring response due to continued injury to the liver, and is featured by excessive accumulation of extracellular matrix (MEC) proteins in hepatic parenchyma. These proteins include collagen, fibronectin, elastin, laminin and proteoglicans, along with the participation of different cell populations within the liver. The main cells responsible for the synthesis of MEC proteins are activated hepatic stellate cells and myofibroblasts, which appear after inflammatory stimuli and are characterized by the expression of alpha-smooth muscle actin (α-SMA). It is known that hepatic fibrosis progression is accompanied by hepatocyte death and its substitution by α-SMA+ fibrogenic cells. Therefore, apoptosis of these fibrogenic cells is of main relevance to fibrosis regression and hepatic regeneration. In the later years, bone marrow stem cell therapy has been used to stimulate hepatic regeneration in different experimental models and clinical protocols. The adult bone marrow mononuclear fraction contains two stem cell populations particularly important in the treatment of diverse hepatic diseases: hematopoietic stem cells and mesenchymal stem cells. The aim of this study was to analyze α-SMA expression and the apoptotic process in hepatic cells during hepatic fibrosis induced by bile duct ligation (BDL) and after bone marrow mononuclear cell (BMMC) transplantation. Livers were collect from rats of the following groups: normal, 14 days of BDL, 21 days of BDL and rats that received BMMC 14 days after BDL and were analyzed after 7 days (total of 21 days of BDL). To quantify α-SMA expression by fibrogenic cells in the experimental groups, immunoperoxidase to α-SMA followed by morphometry in the Image Pro Plus software was performed. To analyze apoptosis in hepatic cells, immunoperoxidase and western blotting (WB) against caspase-3 (apoptotic protein) were used, along with double immunofluorescence against caspase-3 and α-SMA to confocal microscopy analysis. Results of α-SMA quantification by morphometry showed that α-SMA expression increased significantly 14 and 21 days after BDL. However, this expression was significantly decreased in the BMMC treated group, which presented a more preserved hepatic parenchyma in relation to the group with 21 days of BDL. Immunoperoxidase, WB and confocal microscopy results showed that caspase-3 is decreased in fibrotic livers with 14 and 21 days of BDL in comparison to normal group, and was significantly augmented in the BMMC treated group. Confocal microscopy analysis showed that were cells coexpressing α-SMA and caspase-3 in rats treated with BMMC, suggesting fibrogenic cells death and hepatic remodeling.
Silina, Linda. "Targeting TYRO3 : A Novel Strategy to Radiosensitise Bladder Cancer Cells Review of Preclinical Studies to Improve Radiotherapy Response in Muscle-Invasive Bladder Cancer: Lessons and Perspectives TYRO3 Targeting as a Radiosensitizing Strategy in Bladder Cancer TYRO3 as a Molecular Target for Growth Inhibition and Apoptosis Induction in Bladder Cancer." Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASL024.
Повний текст джерелаBladder cancer (BCa) is a major global health problem. It is the fourth most common cancer in men in industrialized countries. 25% of all diagnosed BCa are Muscle-invasive bladder cancers (MIBC) which have poor prognosis. Cystectomy is the standard treatment for MIBC, but for patients with comorbidities it presents significant drawbacks including increased risk of infection and impacted quality of life. Radiotherapy coupled with chemotherapy and tumor transurethral resection has emerged as a promising bladder sparing. Chemotherapy does not spare normal tissue and results in side effects. Therefore, it is of great interest to discover novel radiosensitisation strategies for bladder tumors.TYRO3 is a receptor tyrosine kinase of the TAM family (comprising TYRO3, AXL and MERTK) and is known to regulate diverse biological. TYRO3 is overexpressed in many types of cancer and promotes tumor cell proliferation, survival and resistance to chemotherapy. In addition, higher levels of TYRO3 expression have been associated with decreased overall survival in patients of diverse cancers. However, the role of TYRO3 in BCa has so far not been studied. In this thesis, I investigated:(1)The role of TYRO3 in BCa; (2) The radiosensitising effect of TYRO3 downregulation and inhibition in BCa cells; (3) The effect of TYRO3 downregulation and inhibition on normal human urothelial tissue.We first demonstrated that TYRO3 is overexpressed in 50% of MIBCs. TYRO3 overexpression conferred a TYRO3-dependance to bladder tumor cells for cell growth and viability. Transcriptomic analysis of TYRO3-downregulated cells suggested that TYRO3 signaling controlled cell cycle and protected from apoptosis, which indicated a potential to improve radiation response. TYRO3 downregulation lead to a significantly increased radiosensitivity of BCa cells and conversely, TYRO3-overexpression induced radioresistance. In combination with radiotherapy, TYRO3 dowregulation lead to a cell cycle arrest and a long term persistence of Ionizing Radiation-Induced Foci (IRIF). Finally, I demonstrated that TYRO3 downregulation and inhibition did not impact viability of normal human bladder cells suggesting that inhibiting TYRO3 could improve radiotherapy efficiency while sparing normal surrounding tissues