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Добірка наукової літератури з теми "Multimodal probe"

Автор: Grafiati

Опубліковано: 22 лютого 2025

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Статті в журналах з теми "Multimodal probe"

Beaudette, Kathy, Jiawen Li, Joseph Lamarre, Lucas Majeau, and Caroline Boudoux. "Double-Clad Fiber-Based Multifunctional Biosensors and Multimodal Bioimaging Systems: Technology and Applications." Biosensors 12, no. 2 (February 1, 2022): 90. http://dx.doi.org/10.3390/bios12020090.

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Optical fibers have been used to probe various tissue properties such as temperature, pH, absorption, and scattering. Combining different sensing and imaging modalities within a single fiber allows for increased sensitivity without compromising the compactness of an optical fiber probe. A double-clad fiber (DCF) can sustain concurrent propagation modes (single-mode, through its core, and multimode, through an inner cladding), making DCFs ideally suited for multimodal approaches. This study provides a technological review of how DCFs are used to combine multiple sensing functionalities and imaging modalities. Specifically, we discuss the working principles of DCF-based sensors and relevant instrumentation as well as fiber probe designs and functionalization schemes. Secondly, we review different applications using a DCF-based probe to perform multifunctional sensing and multimodal bioimaging.

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Zang, Xiaonan, Wennan Zhao, Jennifer Toth, Rebecca Bascom, and William Higgins. "Multimodal Registration for Image-Guided EBUS Bronchoscopy." Journal of Imaging 8, no. 7 (July 8, 2022): 189. http://dx.doi.org/10.3390/jimaging8070189.

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The state-of-the-art procedure for examining the lymph nodes in a lung cancer patient involves using an endobronchial ultrasound (EBUS) bronchoscope. The EBUS bronchoscope integrates two modalities into one device: (1) videobronchoscopy, which gives video images of the airway walls; and (2) convex-probe EBUS, which gives 2D fan-shaped views of extraluminal structures situated outside the airways. During the procedure, the physician first employs videobronchoscopy to navigate the device through the airways. Next, upon reaching a given node’s approximate vicinity, the physician probes the airway walls using EBUS to localize the node. Due to the fact that lymph nodes lie beyond the airways, EBUS is essential for confirming a node’s location. Unfortunately, it is well-documented that EBUS is difficult to use. In addition, while new image-guided bronchoscopy systems provide effective guidance for videobronchoscopic navigation, they offer no assistance for guiding EBUS localization. We propose a method for registering a patient’s chest CT scan to live surgical EBUS views, thereby facilitating accurate image-guided EBUS bronchoscopy. The method entails an optimization process that registers CT-based virtual EBUS views to live EBUS probe views. Results using lung cancer patient data show that the method correctly registered 28/28 (100%) lymph nodes scanned by EBUS, with a mean registration time of 3.4 s. In addition, the mean position and direction errors of registered sites were 2.2 mm and 11.8∘, respectively. In addition, sensitivity studies show the method’s robustness to parameter variations. Lastly, we demonstrate the method’s use in an image-guided system designed for guiding both phases of EBUS bronchoscopy.

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Kan-Dapaah, Kwabena, Nima Rahbar, and Wole Soboyejo. "Novel magnetic heating probe for multimodal cancer treatment." Medical Physics 42, no. 5 (April 10, 2015): 2203–11. http://dx.doi.org/10.1118/1.4915955.

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Gabrielli, Luca, and Fabrizio Mancin. "Minimal Self-Immolative Probe for Multimodal Fluoride Detection." Journal of Organic Chemistry 81, no. 22 (October 14, 2016): 10715–20. http://dx.doi.org/10.1021/acs.joc.6b01787.

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Tam, Jenny, Alexander Pilozzi, Umar Mahmood, and Xudong Huang. "Simultaneous Monitoring of Multi-Enzyme Activity and Concentration in Tumor Using a Triply Labeled Fluorescent In Vivo Imaging Probe." International Journal of Molecular Sciences 21, no. 9 (April 27, 2020): 3068. http://dx.doi.org/10.3390/ijms21093068.

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The use of fluorescent imaging probes that monitor the activity of proteases that experience an increase in expression and activity in tumors is well established. These probes can be conjugated to nanoparticles of iron oxide, creating a multimodal probe serving as both a magnetic resonance imaging (MRI) agent and an indicator of local protease activity. Previous works describe probes for cathepsin D (CatD) and metalloproteinase-2 (MMP2) protease activity grafted to cross-linked iron oxide nanoparticles (CLIO). Herein, we have synthesized a triply labeled fluorescent iron oxide nanoparticle molecular imaging (MI) probe, including an AF750 substrate concentration reporter along with probes for cathepsin B (CatB) sand MMP2 protease activity. The reporter provides a baseline signal from which to compare the activity of the two proteases. The activity of the MI probe was verified through incubation with the proteases and tested in vitro using the human HT29 tumor cell line and in vivo using female nude mice injected with HT29 cells. We found the MI probe had the appropriate specificity to the activity of their respective proteases, and the reporter dye did not activate when incubated in the presence of only MMP2 and CatB. Probe fluorescent activity was confirmed in vitro, and reporter signal activation was also noted. The fluorescent activity was also visible in vivo, with injected HT29 cells exhibiting fluorescence, distinguishing them from the rest of the animal. The reporter signal was also observable in vivo, which allowed the signal intensities of the protease probes to be corrected; this is a unique feature of this MI probe design.

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Yadav, Aditya, Chethana Rao, Navneet Chandra Verma, Pushpendra Mani Mishra, and Chayan Kanti Nandi. "Magnetofluorescent Nanoprobe for Multimodal and Multicolor Bioimaging." Molecular Imaging 19 (January 1, 2020): 153601212096947. http://dx.doi.org/10.1177/1536012120969477.

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Although, superparamagnetic iron oxide nanoparticles (SPIONs) have extensively been used as a contrasting agent for magnetic resonance imaging (MRI), the lack of intrinsic fluorescence restricted their application as a multimodal probe, especially in combination with light microscopy. In Addition, the bigger size of the particle renders them incompetent for bioimaging of small organelles. Herein, we report, not only the synthesis of ultrasmall carbon containing magneto-fluorescent SPIONs with size ∼5 nm, but also demonstrate its capability as a multicolor imaging probe. Using MCF-7 and HeLa cell lines, we show that the SPIONs can provide high contrast mulicolor images of the cytoplasm from blue to red region. Further, single particle level photon count data revealed that the SPIONs could efficaciously be utilized in localization based super resolution microscopy in future.

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Ham, Daseul, Su Yong Lee, Sukjune Choi, Ho Jun Oh, Do Young Noh, and Hyon Chol Kang. "Multimodal X-ray probe station at 9C beamline of Pohang Light Source-II." Journal of Synchrotron Radiation 29, no. 4 (June 27, 2022): 1114–21. http://dx.doi.org/10.1107/s1600577522006397.

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In this study, the conceptual design and performance of a multimodal X-ray probe station recently installed at the 9C coherent X-ray scattering beamline of the Pohang Light Source-II are presented. The purpose of this apparatus is to measure coherent X-ray diffraction, X-ray fluorescence and electrical properties simultaneously. A miniature vacuum probe station equipped with a four-point probe was mounted on a six-axis motion hexapod. This can be used to study the structural and chemical evolution of thin films or nanostructures, as well as device performance including electronic transport properties. This probe station also provides the capability of varying sample environments such as gas atmosphere using a mass-flow-control system and sample temperatures up to 600°C using a pyrolytic boron nitride heater. The in situ annealing of ZnO thin films and the performance of ZnO nanostructure-based X-ray photodetectors are discussed. These results demonstrate that a multimodal X-ray probe station can be used for performing in situ and operando experiments to investigate structural phase transitions involving electrical resistivity switching.

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Schanne, Gabrielle, Lucas Henry, How Chee Ong, Andrea Somogyi, Kadda Medjoubi, Nicolas Delsuc, Clotilde Policar, Felipe García, and Helene C. Bertrand. "Rhenium carbonyl complexes bearing methylated triphenylphosphonium cations as antibody-free mitochondria trackers for X-ray fluorescence imaging." Inorganic Chemistry Frontiers 8, no. 16 (2021): 3905–15. http://dx.doi.org/10.1039/d1qi00542a.

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Bang, J. J., S. R. Russell, K. K. Rupp, and S. A. Claridge. "Multimodal scanning probe imaging: nanoscale chemical analysis from biology to renewable energy." Analytical Methods 7, no. 17 (2015): 7106–27. http://dx.doi.org/10.1039/c5ay00507h.

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An, Seong J., Massimiliano Stagi, Travis J. Gould, Yumei Wu, Michael Mlodzianoski, Felix Rivera-Molina, Derek Toomre, et al. "Multimodal imaging of synaptic vesicles with a single probe." Cell Reports Methods 2, no. 4 (April 2022): 100199. http://dx.doi.org/10.1016/j.crmeth.2022.100199.

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Більше джерел

Дисертації з теми "Multimodal probe"

Tran, Vu Long. "Synthesis, Functionalization and Characterization of Ultrasmall Hybrid Silica Nanoparticles for Theranostic Applications." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1020/document.

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Les nanoparticules (NPs) hybrides peuvent combiner les propriétés physiques uniques des éléments inorganiques pour des applications en imagerie et en thérapeutique avec la biocompatibilité des structures organiques. Cependant, leur utilisation en médecine est encore limitée par des risques potentiels de toxicité à long terme. Dans ce contexte, des NPs hybrides ultrafines pouvant être éliminées rapidement par la voie rénale apparaissent comme de bonnes candidates pour la nanomédicine. La NP à base de silice contenant des chélates du gadolinium appelée AGuIX (Activation et Guidage de l’Irradiation par rayon-X) a été développée avec un diamètre hydrodynamique de moins de 5 nm qui lui permet d’être éliminée rapidement via l’urine après injection intraveineuse. Cette NP s’est révélée être une sonde efficace en imagerie multimodale et un amplificateur local en radiothérapie pour le diagnostic et le traitement du cancer. Elle est en train d’être évaluée dans un essai clinique de phase I par radiothérapie des métastases cérébrales (NANO-RAD, NCT02820454). Néanmoins, la synthèse d’AGuIX est un procédé multi-étapes qui est difficilement modulable.Ce manuscrit rapporte, pour la première fois, le développement d’un protocole « one-pot » direct pour des nanoparticules de silice ultrafines (USNP) contenant des chélateurs complexés ou non à partir des précurseurs silanes chélatants moléculaires. Dans ce nouveau protocole, la taille des particules et les types des métaux chélatés peuvent être contrôlés facilement. Certaines des propriétés chimiques des USNP ont été clarifiées davantage pendant ce travail exploratoire. Les particules élaborées ont été caractérisées par différentes techniques analytiques complémentaires. Ces nouvelles nanoparticules USNPs présentent des caractéristiques similaires aux AGuIX en terms de propriétes biologiques et de biodistribution.Dans un second temps, un nouveau protocole de fonctionnalisation d’USNP par des précurseurs silanes chélatants a été développé. Ces chélatants libres fonctionnalisés sur la particule peuvent être alors utilisés afin de complexer des radiométaux pour l’imagerie bimodale. Enfin, d’autres stratégies de fonctionnalisation sont aussi décrites. La nouvelle sonde (17VTh031) combinant un petit chélateur cyclique (NODA) et un fluorophore proche-infrarouge tumeur ciblant (IR783) ainsi que le pyridinium quaternaire ont été greffés sur l’AGuIX pour créer une nouvelle sonde en imagerie multimodale et cibler des tumeurs chondrosarcomes respectivement
Hybrid nanoparticles (NPs) can combine unique physical properties for imaging and therapeutic applications of inorganic elements in bio-friendly organic structures. However, their uses in medicine are limited by the potential risks of long-term toxicities. In this context, ultrasmall renal clearable NPs appear as novel solutions. Silica based NP displaying gadolinium chelates named AGuIX (Activation and Guidance for Irradiation by X-ray) has been developed to have hydrodynamic diameter less than 5 nm which allows rapid elimination through urine after intravenous injection. This NP has been demonstrated as an efficient multimodal imaging probe and a local enhancer for radiotherapy for cancer diagnostics and treatment. It is now being evaluated in a phase I clinical trial by radiotherapy of cerebral metastases (NANO-RAD NCT02820454). Nevertheless, the synthesis of AGuIX implies a multisteps process that can be further improved.This manuscript shows, for the first time, the development of a straightforward one-pot protocol for ultrasmall silica nanoparticles (USNP) containing complexed or non-complexed chelators from molecular chelating silane precursors. In this new protocol, the size of particle and types of metals can be easily tuned. The chemical properties of USNP have been further clarified during this exploratory work. The produced particles have been characterized by different complimentary analytical techniques. These new nanoparticles USNPs show similar characteristics to AGuIX in terms of biological properties and biodistribution.Secondly, a new protocol of functionalization for USNP by chelating silane precursors has been developed. These functionalized free chelators on the particle can be used then to complex radiometals for bimodal imaging applications. Finally, other functionalization strategies have also been described. New probe (17VTh031) combining small cyclic chelator (NODA) and tumor targeting near-infrared fluorophore (IR783) as well as quaternary pyridinium have been grafted on AGuIX for creating new multimodal imaging probe and targeting chondrosarcoma tumors respectively

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Henry, Lucas. "Sondes multimodales Re(CO)₃ pour la détection intracellulaire : synthèse, étude physico-chimique et sur cellules." Thesis, Paris Sciences et Lettres (ComUE), 2019. http://www.theses.fr/2019PSLEE026.

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Les questions de la détection intracellulaire et de la localisation en milieu cellulaire de complexes de métaux de transition sont cruciales dans le contexte du développement de complexes métalliques en biologie et médecine. En effet, pour être pharmacologiquement actif, un composé doit atteindre sa cible cellulaire : la localisation est donc un paramètre clef de l’activité. Pour l’étudier, il est nécessaire de disposer de sondes et de techniques d’imagerie cellulaire. Nous avons montré qu’il est possible d’utiliser les métaux carbonyle comme des sondes multimodales en fluorescence classique, infrarouge et fluorescence X, la multimodalité étant portée par un seul cœur moléculaire de type (L)Re(CO)₃, L étant un ligand de type bipyridine. Le terme SCoMPI a été introduit pour ces « Single Core Multimodal Probes for Imaging ». Dans le cadre de cette thèse, nous avons préparé et imagé des sondes greffables pour suivre des objets d’intérêt biologique (acide hyaluronique, ferrocifènes) ainsi que des marqueurs en fluorescence X des organites intracellulaires. Dans ce cas, des groupes vecteurs (TPP, peptide...) ont été greffés sur des unités de type Re-tricarbonyle et ces espèces ont été caractérisées en contexte biologique par différentes techniques analytiques et imagées par différentes techniques de microscopie
Questions of intracellular detection and cell-mediated localization of transition metal complexes are major in the context of metal complex development in biology and medicine. A compound must reach its cellular target to be pharmacologi- cally active: the localization is therefore a key parameter of activity. To study it, it is necessary to have probes and cell imaging techniques. Metal carbonyls are ideal candidates for multimodal bioimaging in fluorescence, infrared and X-ray fluorescence imaging. As they have been developed as "Single Core Multimodal Probe for Imaging" (SCoMPI), this thesis deals with the development, the study and the use of a rhenium-tricarbonyl unit [LRe(CO)₃X] (L = pyridyl-1,2,3-triazole, X = Cl−) as X-ray fluorescence probe for cellular imaging. We conjugated the Re(CO)₃ graftable probe to compounds of interest (hyaluronic acid, ferrocifens) in order to study their penetration and location in cells or in the skin, and we modified Re(CO)3 conjugates by organelles targeting groups (TPP, peptide, etc.) in order to develop species that were characterized in biological context by different analytical techniques and imaged by different microscopy techniques

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Cave, Charlotte. "Aza-BODIPY-métaux : vers une nouvelle classe de sondes multimodales fluorescentes." Electronic Thesis or Diss., Bourgogne Franche-Comté, 2024. http://www.theses.fr/2024UBFCK075.

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De nos jours, l’imagerie moléculaire est devenue un allié indispensable de la médecine, notamment dans le traitement du cancer, que ce soit pour l’établissement d’un diagnostic, l’aide au choix d’un traitement personnalisé voire plus récemment pour la chirurgie assistée par fluorescence (FGS). L’imagerie optique de fluorescence prend de l’essor ces dernières années pour des applications in vivo en pré clinique voire en clinique grâce à la FGS et l’utilisation de fluorophore NIR-I (700-900 nm). Néanmoins, l’utilisation de cette technique est handicapée par ses limites de pénétration à travers les tissus biologiques. C’est pourquoi, la combinaison de l’imagerie de fluorescence à l’imagerie radioisotopique (TEP ou TEMP) permet de corréler avec fiabilité les images pré opératoires et celles révélées au moment de la résection de la tumeur. En ce sens, le développement de sondes bimodales fait l’objet de nombreux défis en termes de conception et de synthèse. Les azaBODIPYs, qui sont des fluorophores stables chimiquement et photochimiquement, sont au cœur de l’expertise de notre laboratoire. Ainsi, une sonde azaBODIPY OI/TEP bioconjugable et hydroluble a pu êre développée lors de travaux précédents. Néanmoins, la synthèse de cette molécule est complexe et chronophage. De plus, les deux modalités étant éloignées l’une de l’autre ont un risque potentiel d’être dissociées si la molécule est dégradée sans que cela ne soit détectable. C’est pourquoi cette thèse avait pour objectif principal de concevoir une nouvelle classe de sondes bimodales plus compactes et intelligentes, où l’élément radioisotopique est directement intégré dans le fluorophore. Cette combinaison azaBODIPY/chimie de coordination implique le développement de systèmes stables, biocompatibles et répondant aux exigences d’un radiomarquage. Ce manuscrit décrit la synthèse de nouveaux ligands, des complexes associés, ainsi que leur caractérisation chimique et photophysique. Il détaille le travail d’optimisation des synthèses mais aussi d’hydrosolubilisation des systèmes et des voies possibles pour les vectoriser. Un chapitre est consacré à l’évaluation biologique des systèmes les plus prometteurs et leur validation in vivo comme sondes bimodales
Nowadays, molecular imaging has become an indispensable ally in medicine, particularly in cancer treatment, supporting diagnosic accuracy, personalized treatment planning, and, more recently, fluorescence-guided surgery (FGS). In recent years, fluorescence optical imaging has gained interest for in vivo pre-clinical and even clinical applications, particularly due to FGS and the use of NIR-I fluorophores (700-900 nm). However, the effectiveness of this technique is limited by its low tissue penetration depth. To adress this limitation, combining fluorescence imaging with radioisotopic imaging (PET or SPECT) enables reliable correlation between preoperative imaging and peroperative tumor visualization. That is why, bimodal probes development is a challenge in terms of design and synthesis. AzaBODIPYs, known for their chemical and photochemical stability, form a core area of expertise in our laboratory. In previous work, our team developped a bioconjugable, water-soluble OI/TEP probe. However, the synthesis of this molecule is complex and time-consuming. Furthemore, the separation of the two modalities in the probe increases the risk of dissociation upon degradation, potentially impacting signal reliability without this being detectable. Therefore, the primary goal of this thesis was to design a new class of more compact and smart bimodal probes, where the radioisotopic element is directly integrated into the fluorophore. This azaBODIPY/coordination chemistry approach aims to create stable, biocompatible systems compatible with radiolabeling requirements. This manuscript describes the synthesis of new ligands and their corresponding complexes as well as their chemical and photophysical characterization. It also covers synthetic optimisation efforts, water-solubilization of the systems, and potential targeting strategies. A dedicated chapter focuses on the biological evaluation of the most promising systems and their in vivo validation as bimodal probes

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Clède, Sylvain. "From metal carbonyls to single core multimodal probes for imaging." Paris 6, 2013. http://www.theses.fr/2013PA066576.

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Les métaux carbonyles, de par leurs propriétés vibrationnelle et de luminescence, sont des candidats idéaux afin de réaliser une imagerie bimodale cellulaire. Le fil conducteur de cette thèse a été la mise au point, l’étude et l’utilisation d’un cœur rhénium tris-carbonyle [LRe(CO)3X] (L = pyridyl-1,2,3-triazole et X = Cl-) dit SCoMPI pour « Single Core Multimodal Probe for Imaging », combinant les modalités IR et de luminescence sur une structure moléculaire unique, stable en milieu biologique. Des cartes IR et de luminescence ont été réalisées sur des cellules incubées avec un dérivé SCoMPI. Leur correspondance a démontré l’intégrité en milieu cellulaire et la pertinence en tant qu’agent bimodal d’une telle unité. Il était alors possible de l’envisager comme une plateforme de spectroscopie corrélative greffable. Une première application à la détection d’un dérivé œstrogénique a mis en évidence tout son potentiel pour le traçage de molécules. L’étude approfondie de l’influence de substituants du ligand L sur la pénétration cellulaire de sondes SCoMPI a permis de mieux comprendre les paramètres influant sur leur internalisation. Le suivi IR/luminescence en temps réel de sa translocation a montré l’intérêt de ces objets pour l’imagerie de cellules vivantes. Parallèlement à ces travaux, nous avons confirmé qu’il est pertinent de considérer des rapports de bandes IR en vue de détecter des organites, sans recours à un marquage exogène. Grâce à ses nombreux atouts (faible énergie mise en jeu, pénétration accrue, signature propre à chaque fonction chimique), la spectroscopie IR est amenée à être davantage utilisée pour la résolution de problématiques biologiques
Metal carbonyls are ideal candidates for bimodal bio-imaging due to their appropriate vibrational and luminescence properties. The main focus of this work has been the development, the study and the use of a rhenium tris-carbonyl unit [LRe(CO)3X] (L = pyridyl-1,2,3-triazole, X = Cl-), named SCoMPI for “Single Core Multimodal Probe for Imaging”, combining IR and luminescent modalities on a unique molecular moiety, robust in biological media. Since IR and luminescent sub-cellular mappings of the first SCoMPI compound were consistent, its integrity and relevance as a bimodal imaging agent were demonstrated. A first bio-application to track an estrogen derivative showed that SCoMPIs have a great potential as luminescent and vibrational tags. The in-depth study of the influence of pendant groups on its cellular uptake allowed for a better understanding of the parameters involved. Successful bimodal live-cell imaging highlighted that SCoMPIs could be used as imaging agents in living cells. In parallel, the analysis of minute changes in intracellular spectra by synchrotron-based IR spectromicroscopy confirmed the relevance of considering specific IR band-ratios to detect organelles, with no need of exogenous staining. Thanks to its multiple assets (low energy involved, deep penetration, specific signature of each chemical function), the IR spectroscopy tends to be more used in the future for biological applications

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Fediv, V. I. "Quantum dot as the basis of multimodal molecular imaging probes." Thesis, БДМУ, 2021. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/18844.

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Magnusson, Karin. "Poly-and oligothiophenes : Optical probes for multimodal fluorescent assessment of biological processes." Doctoral thesis, Linköpings universitet, Kemi, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-121815.

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Анотація:

One interesting class of molecules in the research field of imaging biological processes is luminescent conjugated polythiophenes, LCPs. These fluorescent probes have a flexible backbone consisting of repetitive thiophene units. Due to this backbone, the probes possess unique abilities to give rise to different spectral signatures depending on their target and environment. LCPs are a polydispersed material meaning there is an uneven distribution of lengths of the probe. Recently, monodispersed chemically well-defined material denoted luminescent conjugated oligothiophenes, LCOs, with an exact number of repetitive units and distinct sidechain functionalities along the backbone has been developed. LCOs have the advantages of being smaller which leads to higher ability to cross the blood brain barrier. The synthesis of minor chemical alterations is also more simplified due to the well-defined materials. During my doctoral studies I have used both LCPs and LCOs to study biological processes such as conformational variation of protein aggregates in prion diseases and cellular uptake in normal cells and cancer cells. The research has generally been based on the probes capability to emit light upon irradiation and the interaction with their targets has mainly been assessed through variations in fluorescence intensity, emission-and excitation profiles and fluorescence lifetime decay. These studies verified the utility of LCPs and LCOs for staining and discrimination of both prion strains and cell phenotypes. The results also demonstrated the pronounced influence minor chemical modifications have on the LCO´s staining capacity.

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Vicente, Juvinch R. "Photo-driven Processes in Lead Halide Perovskites Probed by Multimodal Photoluminescence Microscopy." Ohio University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1585838644331732.

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Li, Zizhen. "Synthesizing Multimodal Imaging Probes and Their Application in Non-Invasive Axonal Tracing by Magnetic Resonance Imaging." Thesis, Université d'Ottawa / University of Ottawa, 2016. http://hdl.handle.net/10393/34414.

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Imaging techniques have become much more in demand in modern medicine, especially in fields of disease prognosis, diagnosis and therapeutics. This is because a better understanding of different diseases, characteristics of each patient and further optimizing treatment planning, are all enhanced by advanced imaging techniques. Since each imaging modality has its own merits and intrinsic limitations, combining two or more complementary imaging modalities has become an interesting research area. In this study, gadolinium (Gd3+) doped CdTe quantum dots (QDs) were synthesized and used as multimodal imaging probes of two highly complementary imaging modalities: optical imaging and magnetic resonance imaging. The new imaging probes were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), UV-vis absorbance spectra, fluorescence spectra (FL) and magnetic resonance imaging (MRI). The optical / MRI imaging probes were further functionalized by conjugating with the axonal tracer dextran amine (10 kDa) for non-invasive axonal tracing observations. Biocompatibility and MRI contrast effect of prepared multimodal imaging probes were investigated by in vitro cell experiments and MRI scanner. Ultimately, it is hoped that this imaging probe will help us better understand the regeneration mechanisms in real time without sacrificing animals at intervening time-points.

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Travaillot, Thomas. "Caractérisation mécanique des matériaux élastiques à l'échelle locale par microscopie à pointe vibrante : Approche multimodale et mesure de champs." Thesis, Besançon, 2014. http://www.theses.fr/2014BESA2011/document.

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Ces travaux de thèse proposent une amélioration du Scanning Microdeformation Microscope (SMM),un microscope à sonde locale, pour la caractérisation mécanique élastique des matériaux à l’échellelocale. Il est montré qu’en utilisant n > 2 modes de résonance du SMM, il est possible de découplerles mesures du module de Young et du coefficient de Poisson d’un matériau isotrope.Une étude des modes du résonateur a permis d’enrichir son modèle afin qu’il puisse modélisern > 2 modes. Des procédures ont été développées pour identifier les paramètres de ce modèle etles constantes élastiques des matériaux à partir de n > 2 fréquences de résonance. Enfin, ces procéduresont été appliquées à des exemples de caractérisation à l’échelle locale afin de valider laméthode et d’en exhiber les possibilités et les limites.Pour gagner en robustesse et se diriger vers la caractérisation des matériaux anisotropes, un systèmed’imagerie interférométrique en lumière polarisée, permettant la mesure du champ de rotationde surfaces réfléchissantes dans une direction particulière, a été développé pour être intégré auSMM. Son prisme biréfringent à gradient uniaxial d’indice lui confère sa sensibilité à la rotation. Cesystème est capable de mesurer un champ de rotation localisé comme c’est le cas au voisinage dela pointe du SMM. Il a aussi montré son intérêt dans les cas où l’effet d’échelle rend particulièrementintéressante la mesure de la rotation
This work proposes an improvement of the Scanning Microdeformation Microscope (SMM), a scanningprobe microscope, for the mechanical elastic characterization of materials at local scale. It demonstratesthat using n > 2 SMM resonance frequencies allows to decouple Young’s modulus andPoisson’s ratio values for an isotropic material.The mechanical description of the resonator has been enriched in order to allow for an accuratemodeling over a wide frequency range. Procedures have been developed to identify the modellingparameters and the elastic constants of the materials from n > 2 resonant frequencies. Finally, theseprocedures have been applied to the characterization of various materials at local scale in order tovalidate the method and to present possibilities and limits.To improve robustness and move towards the characterization of anisotropic materials, a polarizedlightimaging interferometer was developed to measure the rotation field of reflecting surfaces in aparticular direction. The sensitivity to the rotation originates from a homemade birefringent prism withuniaxial gradient of refractive index. This system is able to measure a localized rotation field as it isinduced in the vicinity of the tip of the SMM. Its interest is also demonstrated in cases in which scaleeffects make the rotation measurement preferable to the out-of-plane displacement measurement

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Vorng, Jean-luc. "Synthèse de sondes moléculaires pour l'imagerie multimodale et multi-échelle appliquée en science du vivant." Thesis, Rennes 1, 2013. http://www.theses.fr/2013REN1S123/document.

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Les travaux décrits dans ce mémoire ont pour objectif d'imager des cellules par des techniques d'imagerie complémentaires à la spectroscopie de fluorescence : la micro-spectroscopie Raman et l'imagerie NanoSIMS. Le présent manuscrit se divise en quatre chapitres et présente les différents aspects menant à la mise en place de ce projet. Le premier chapitre du manuscrit a pour but de positionner le projet d'un point de vue biologique. Ce chapitre présente l'organite que nous souhaitions marquer, le nucléole, et la façon dont nous avons procédé à sa localisation par l'intermédiaire de sondes moléculaires. Le marquage que nous avons choisi est un immuno-marquage indirect faisant intervenir deux anticorps : un anticorps primaire spécifique à la nucléophosmine, protéine majoritairement présente dans le nucléole et un anticorps secondaire, reconnaissant l'anticorps primaire, marqué par une sonde moléculaire d'intérêt. Ce chapitre a également permis de montrer par une analyse en immunofluorescence l'absence d'interférence des sondes moléculaires dans le cadre de ce marquage. Le second chapitre se focalise sur la synthèse des sondes moléculaires permettant le marquage des anticorps secondaires. Les sondes envisagées pour ce marquage possèdent un groupement ester de succinimide réactif capable de "s'accrocher" de manière covalente aux fonctions amines de l'anticorps secondaire et un ou plusieurs groupements fonctionnels visible par la technique d'imagerie voulue. Trois catégories de sondes ont été préparées et dépendent de la technique d'imagerie employée. Les sondes Raman comportant une triple liaison carbone-carbone visible par cette technique, les sondes NanoSIMS dont l'acquisition d'image sera possible par les halogènes présents dans la structure et les sondes bimodales comportant les deux éléments dans la même structure. Les sondes bimodales sont obtenues par le couplage pallado-catalysé de Sonogashira. Dans la dernière partie du chapitre, une nouvelle série de sonde a été envisagée dans le cadre d'une application future : le marquage de l'ATP-ϒ-SH grâce à une fonction mésylate. Les deux derniers chapitres ont pour but de mettre en application tout ce qui a été présenté dans les chapitres précédents. Les deux techniques employées permettent d'accéder à des types d'informations différentes : la spectroscopie Raman donne accès aux modes de vibrations d'une molécule tandis que l'imagerie NanoSIMS permet d'obtenir des informations élémentaires et isotopiques. Nous présenterons le cheminement suivi pour imager des cellules via leurs constituants par micro-spectroscopie Raman et imagerie NanoSIMS via les sondes moléculaires introduites
Life sciences imaging are widely used for different applications, they are interested in medical diagnosis as well as basic research. In cells biology, fluorescence microscopy is mainly used for organelles observation at sub-cellular scale. However, techniques based on fluorescence phenomena are limited by some drawbacks like technical resolution, fluorescent dye degradation and the number of channels, which can be visualized. In this context, the exploration and the development of new way for image acquisition are considered as an experimental and technical scientific challenge. Furthermore, it can lead to complementary technique to fluorescence microscopy.This PhD thesis is a life science imaging project development and application allowing image acquisition base on molecular vibrations phenomena and elementary analysis in cells. Two techniques have been chosen in relation to both specificity: micro Raman spectroscopy and NanoSIMS imaging. Micro Raman spectroscopy allows the observation of molecular vibration mode at micron scale and NanoSIMS leads to elementary and isotopic sample information at sub-micronic scale. Combination of both techniques will lead to multi-scale and multi-modal imaging of biological samples. Molecular probes designing and synthesis for both techniques were used to visualize an organelle inside the nucleus: the nucleolus. Nucleolus has a key role in ribosomal RNA transcription and researchers shows some interest in the study of this organelle for his multifunctional role like ribosome biogenesis and nuclear organization. An immuno-labelling method combine with the introduction of molecular probes will allow nucleolus imaging by micro-Raman spectroscopy and NanoSIMS spectrometry. This immuno-labelling is specific to a phosphoprotein mainly localized inside the nucleolus: the nucleophosmin (NPM). In this project, the introduction of molecular probes in an immuno-labelling will act as a Raman Tag or a NanoSIMS tag for NPM's nucleolus observation and studies.This work at the interface between different fields: chemistry, biology and physics shows all the aspect of this project starting from molecular probes synthesis, immuno-labelling methods uses to direct application of both Raman and NanoSIMS techniques

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Книги з теми "Multimodal probe"

K, Sahoo N., and Bhabha Atomic Research Centre, eds. Multimode scanning probe microscopy in characterizing precision optical thin films and multilayers. Mumbai: Bhabha Atomic Research Centre, 2001.

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A, Lock James, and United States. National Aeronautics and Space Administration., eds. Calibration of the forward-scattering spectrometer probe: Modeling scattering from a multimode laser beam. [Washington, DC: National Aeronautics and Space Administration, 1993.

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Calibration of the forward-scattering spectrometer probe: Modeling scattering from a multimode laser beam. [Washington, DC: National Aeronautics and Space Administration, 1993.

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Yu, Ning. The Moral Metaphor System. Oxford University PressOxford, 2022. http://dx.doi.org/10.1093/oso/9780192866325.001.0001.

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Abstract This book presents a study of moral metaphors in English and Chinese, applying cognitive linguistics’ conceptual metaphor theory (CMT) to a comparative study of linguistic manifestation of the moral metaphor system rooted in the domains of bodily and physical experience. It intends to shed light on the metaphorical nature of moral cognition and how it is systematically manifested in language. The study sets out with the central goal to contribute to the discovery of potential commonalities that define moral cognition in general as well as the detection of possible differences that characterize distinct cultures concerning moral cognition. It probes into moral cognition at the cultural level as reflected in language, based on linguistic evidence from both English and Chinese and, to a limited extent, multimodal evidence from the corresponding cultures. The moral metaphor system under study is taken as consisting of three major subsystems, named in a shorthand fashion as “physical”, “visual”, and “spatial”. The three subsystems are clusters of conceptual metaphors, whose source concepts are from domains of embodied experiences in the physical world, and which are formulated in contrastive categories with bipolar values for the target concepts moral and immoral. The study is characterized by two keywords: system and systematicity. The former refers to the fact that metaphors (conceptual and linguistic) are connected in networks; the latter refers to the need that metaphors should be studied in such networks.

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Частини книг з теми "Multimodal probe"

Men, Qianhui, Clare Teng, Lior Drukker, Aris T. Papageorghiou, and J. Alison Noble. "Multimodal-GuideNet: Gaze-Probe Bidirectional Guidance in Obstetric Ultrasound Scanning." In Lecture Notes in Computer Science, 94–103. Cham: Springer Nature Switzerland, 2022. http://dx.doi.org/10.1007/978-3-031-16449-1_10.

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Del Dottore, Emanuela, Alessio Mondini, Davide Bray, and Barbara Mazzolai. "Miniature Soil Moisture Sensors for a Root-Inspired Burrowing Growing Robot." In Biomimetic and Biohybrid Systems, 184–96. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-38857-6_15.

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AbstractThis paper shows the implementation of miniature sensors for soil moisture measurement and their integration in a root-inspired burrowing growing robot. Three kinds of sensors are combined to estimate the water content in soil: a resistivity sensor composed of two brass electrodes, a commercial air humidity sensor interfaced with the soil by a filter membrane of PTFE with polyester scrim, and an RGB sensor used for visible reflectance spectroscopy. We show their integration and embeddability in a burrowing growing robot based on additive manufacturing with a 4 cm probe diameter. The multimodal sensing strategy has been characterized and tested in clay and sand medium at different water content. Results show that the resistive sensor works in all the tested ranges but is prone to failure due to electrode-soil contact issues. The air humidity sensor works accurately in a range of water content less than 5% (dry conditions), and the RGB sensor works in the 5–20% range. We propose a statistical approach for soil moisture estimation that combines all three technologies and demonstrate that we can accurately predict the water content in our experimental soils, clay and sand, with better performance in clay (Root Mean Square Error, RMSE = 0.38). The proposed miniaturized multimodal sensing strategy can enable long-term, in-situ soil moisture monitoring functionalities in self-deployable robots for precision agriculture and forestry applications.

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Zhang, Chao, and Jiawei Han. "Data Mining and Knowledge Discovery." In Urban Informatics, 797–814. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-8983-6_42.

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AbstractOur physical world is being projected into online cyberspace at an unprecedented rate. People nowadays visit different places and leave behind them million-scale digital traces such as tweets, check-ins, Yelp reviews, and Uber trajectories. Such digital data are a result of social sensing: namely people act as human sensors that probe different places in the physical world and share their activities online. The availability of massive social-sensing data provides a unique opportunity for understanding urban space in a data-driven manner and improving many urban computing applications, ranging from urban planning and traffic scheduling to disaster control and trip planning. In this chapter, we present recent developments in data-mining techniques for urban activity modeling, a fundamental task for extracting useful urban knowledge from social-sensing data. We first describe traditional approaches to urban activity modeling, including pattern discovery methods and statistical models. Then, we present the latest developments in multimodal embedding techniques for this task, which learns vector representations for different modalities to model people's spatiotemporal activities. We study the empirical performance of these methods and demonstrate how data-mining techniques can be successfully applied to social-sensing data to extract actionable knowledge and facilitate downstream applications.

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Tanaka, Tetsu, Norihiro Katayama, Kazuhiro Sakamoto, Makoto Osanai, and Hajime Mushiake. "Multimodal Functional Analysis Platform: 2. Development of Si Opto-Electro Multifunctional Neural Probe with Multiple Optical Waveguides and Embedded Optical Fiber for Optogenetics." In Advances in Experimental Medicine and Biology, 481–91. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-8763-4_32.

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Sunwoo, Sung Hyuk, and Tae-il Kim. "Materials and Designs for Multimodal Flexible Neural Probes." In Stretchable Bioelectronics for Medical Devices and Systems, 293–308. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-28694-5_15.

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Obokata, Tomio, Stefan Bopp, and Cam Tropea. "Two-Point Adapter for LDA Probe Using Multimode Fibers." In Applications of Laser Techniques to Fluid Mechanics, 347–63. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-61254-1_18.

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Robert-Murail, Constance. ""Smuggling in Accidental Poetry": Cognitive and Stylistic Strategies of a Stammering Teen in David Mitchell's Black Swan Green." In Powerful Prose, 231–48. Bielefeld, Germany: transcript Verlag, 2021. http://dx.doi.org/10.14361/9783839458808-014.

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In this article, Constance Robert-Murail will explore the poetic »accidents« at work in two extracts of Black Swan Green (2006) by David Mitchell. The novel tells the trials and musings of Jason Taylor, a thoughtful 13-year-old growing up in a backwater town full of strange neighbours and middle-school bullies. Throughout the year 1982, the reader witnesses Jason mediating between the various personae of his fragmented identity: Unborn Twin, his faint-hearted alter ego; Eliot Bolivar, the nom-de-plume he uses to write poems for the local parish newspaper; and, most importantly, Hangman, a malignant personification of his stammer. According to Garan Holcombe, David Mitchell's own experience of stammering has provided the novelist with a particular »sensitivity toward the formal necessity of coherence and structure« (Holcombe, 2013). The extract I have decided to focus on dramatises the onset of Jason's speech impediment and acts as a »high emotional intensity passage« (Toolan, 2012) within the structure of the coming-of-age narrative. A close stylistic reading of this particular text highlights the juxtaposition of Jason's pathological speechlessness and his bustling, bubbling inner monologue. This opposition elicits a physical reaction within the reader, caught between frustration and delectation. I would argue that the multimodal nature of the extract generates what Pierre-Louis Patoine has called a »somesthetic« effect on the reader (Patoine, 2016). Stuttering, according to Professor Mark Onslow, is »an idiosyncratic disorder.« (Onslow, 2017). Word avoidance has led Jason to create his own grammar and lexicon: his youthful voice and palliative strategies allow Mitchell to smuggle in moments of »accidental« poetry. The cognitive exploration of Jason's stammer stands both at the core of the reader's response and at the centre of Mitchell's powerful poetics-and it is, last but not least, devastatingly funny.

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Eckermann, Amanda L., Daniel J. Mastarone, and Thomas J. Meade. "Chemical Strategies for the Development of Multimodal Imaging Probes Using Nanoparticles." In The Chemistry of Molecular Imaging, 355–87. Hoboken, NJ: John Wiley & Sons, Inc, 2014. http://dx.doi.org/10.1002/9781118854754.ch16.

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Zhao, Tianrui, and Wenfeng Xia. "Optical Wavefront Shaping in Biomedical Photoacoustics." In Biomedical Photoacoustics, 231–52. Cham: Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-61411-8_8.

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AbstractOptical wavefront shaping is an emerging technique that controls light transportation in disordered media. It has attracted significant interest in the photoacoustics community in recent years. On one hand, photoacoustic signals can provide feedback on local light intensity to guide optical wavefront shaping non-invasively for focusing light through optically diffusive media. On the other hand, the wavefront shaping technique can be used to focus light through multimode fibres to achieve ultrathin photoacoustic endomicroscopy probes. This chapter reviews the latest research that lies at the intersection of wavefront shaping and photoacoustics, encompassing photoacoustic-guided wavefront shaping and multimode fibre-based photoacoustic endomicroscopy techniques.

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Dolphin, Rian, Barry Smyth, and Ruihai Dong. "A Machine Learning Approach to Industry Classification in Financial Markets." In Communications in Computer and Information Science, 81–94. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-26438-2_7.

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AbstractIndustry classification schemes provide a taxonomy for segmenting companies based on their business activities. They are relied upon in industry and academia as an integral component of many types of financial and economic analysis. However, even modern classification schemes have failed to embrace the era of big data and remain a largely subjective undertaking prone to inconsistency and misclassification. To address this, we propose a multimodal neural model for training company embeddings, which harnesses the dynamics of both historical pricing data and financial news to learn objective company representations that capture nuanced relationships. We explain our approach in detail and highlight the utility of the embeddings through several case studies and application to the downstream task of industry classification.

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Тези доповідей конференцій з теми "Multimodal probe"

Wang, Yihan, Shaobai Li, Hongzhang Ma, Bofan Song, and Rongguang Liang. "Multimodal OCT intraoral probe for oral cancer early detection." In Multimodal Biomedical Imaging XX, edited by Xavier Intes, Marien Ochoa, and Mohammad A. Yaseen, 9. SPIE, 2025. https://doi.org/10.1117/12.3042212.

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Velázquez-Benítez, Amado M., Eloy Montesinos-Garrido, Itzel Garnica-Palafox, Thomas Bradley, Naser Qureshi, and Chigo Okonkwo. "Fiber optic probe for complex light beams multiplexing single- and few-mode signals." In Latin America Optics and Photonics Conference, Tu5A.4. Washington, D.C.: Optica Publishing Group, 2024. https://doi.org/10.1364/laop.2024.tu5a.4.

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A fiber bundle device capable to generate multiple spots, single and multimode, that can be used for different applications requiring complex light patterns. The device is simple in fabrication based on existing fiber bundle technology.

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Huang, Ziyi, Chenyang Su, Dejun Liu, Yalong Tai, Longhui Huang, Weijia Bao, Yiping Wang, and Changrui Liao. "All-Fiber Coaxial Focus Multimode Beam Probe for Optical Coherence Tomography." In 2024 22nd International Conference on Optical Communications and Networks (ICOCN), 1–3. IEEE, 2024. http://dx.doi.org/10.1109/icocn63276.2024.10648353.

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Wang, Lele, Yiwei Zhang, Dan Li, Ping Yan, and Qirong Xiao. "Lensless microimaging via a multimode fiber probe based on compression sampled speckles." In CLEO: Science and Innovations, SF1A.5. Washington, D.C.: Optica Publishing Group, 2024. http://dx.doi.org/10.1364/cleo_si.2024.sf1a.5.

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The all-fiber lensless microimaging scheme was experimentally demonstrated for the first time. Natural scenes reconstruction and distance detection are implemented with dual networks and partially diffuse speckles. Highly integrated structure is suitable for implantable micro-endoscopes.

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Akram, Mohammad Makhdoumi, Farshid Shateri, Abdolkhalegh Mohammadi, Alireza Geravand, Wei Shi, and Benoit Gosselin. "Implantable Neural Probe with Thermo-Optic Switches Based on Multimode Interference (MMI) in Thermogenetic Application." In 2024 22nd IEEE Interregional NEWCAS Conference (NEWCAS), 148–52. IEEE, 2024. http://dx.doi.org/10.1109/newcas58973.2024.10666322.

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Li, Shaobai, Wenjun Kang, Yihan Wang, Hongzhang Ma, Bofan Song, and Rongguang Liang. "Development of a multimodal intraoral probe for oral cancer." In Multimodal Biomedical Imaging XIX, edited by Fred S. Azar and Xavier Intes. SPIE, 2024. http://dx.doi.org/10.1117/12.3007491.

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Yoon, Yeoreum, Won Hyuk Jang, and Kihean Kim. "Optical multimodal probe for image guided surgery." In 2014 11th International Conference on Ubiquitous Robots and Ambient Intelligence (URAI). IEEE, 2014. http://dx.doi.org/10.1109/urai.2014.7057388.

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Baria, Enrico, Simone Morselli, Mauro Gacci, Francesco S. Pavone, Riccardo Fantechi, Riccardo Cicchi, Suresh Anand, and Marco Carini. "Tumour detection and staging through multimodal fibre-probe spectroscopy." In Biophotonics: Photonic Solutions for Better Health Care, edited by Jürgen Popp, Valery V. Tuchin, and Francesco S. Pavone. SPIE, 2018. http://dx.doi.org/10.1117/12.2317996.

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Choi, Minseog, Seungwan Lee, Jong-hyeon Chang, Eunsung Lee, Kyu-Dong Jung, and Woonbae Kim. "Design of an optical probe compatible for multimodal imaging." In SPIE Optical Engineering + Applications, edited by G. Groot Gregory and Arthur J. Davis. SPIE, 2012. http://dx.doi.org/10.1117/12.929477.

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Dai, Xianjin, Hao Yang, Jianbo Tang, Can Duan, Quentin Tanguy, Huikai Xie, and Huabei Jiang. "Miniature multimodal endoscopic probe based on double-clad fiber." In SPIE BiOS, edited by Guillermo J. Tearney and Thomas D. Wang. SPIE, 2017. http://dx.doi.org/10.1117/12.2251510.

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Звіти організацій з теми "Multimodal probe"

Enikov, Eniko T. Multimode Scanning Probe Microscope System for Nanocomposite Actuators. Fort Belvoir, VA: Defense Technical Information Center, July 2002. http://dx.doi.org/10.21236/ada406940.

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