Добірка наукової літератури з теми "Multi-omics Integration"

Abstract Oncogenesis and cancer can arise as a consequence of a wide range of genomic aberrations including mutations, copy number alterations, expression changes and epigenetic modifications encompassing multiple omics layers. Integrating genomic, transcriptomic, proteomic and epigenomic datasets via multi-omics analysis provides the opportunity to derive a deeper and holistic understanding of the development and progression of cancer. There are two primary approaches to integrating multi-omics data: multi-staged (focused on identifying genes driving cancer) and meta-dimensional (focused on establishing clinically relevant tumour or sample classifications). A number of ready-to-use bioinformatics tools are available to perform both multi-staged and meta-dimensional integration of multi-omics data. In this study, we compared nine different integration tools using real and simulated cancer datasets. The performance of the multi-staged integration tools were assessed at the gene, function and pathway levels, while meta-dimensional integration tools were assessed based on the sample classification performance. Additionally, we discuss the influence of factors such as data representation, sample size, signal and noise on multi-omics data integration. Our results provide current and much needed guidance regarding selection and use of the most appropriate and best performing multi-omics integration tools.

Chronic obstructive pulmonary disease (COPD) is an umbrella diagnosis caused by a multitude of underlying mechanisms, and molecular sub-phenotyping is needed to develop molecular diagnostic/prognostic tools and efficacious treatments.The objective of these studies was to investigate whether multi-omics integration improves the accuracy of molecular classification of COPD in small cohorts.Nine omics data blocks (comprising mRNA, micro RNA, proteomes and metabolomes) collected from several anatomical locations from 52 female subjects were integrated by similarity network fusion (SNF). Multi-omics integration significantly improved the accuracy of group classification of COPD patients from healthy never-smokers and from smokers with normal spirometry, reducing required group sizes from n=30 to n=6 at 95% power. Seven different combinations of four to seven omics platforms achieved >95% accuracy.For the first time, a quantitative relationship between multi-omics data integration and accuracy of data-driven classification power has been demonstrated across nine omics data blocks. Integrating five to seven omics data blocks enabled 100% correct classification of COPD diagnosis with groups as small as n=6 individuals, despite strong confounding effects of current smoking. These results can serve as guidelines for the design of future systems-based multi-omics investigations, with indications that integrating five to six data blocks from several molecular levels and anatomical locations suffices to facilitate unsupervised molecular classification in small cohorts.

High-throughput technologies have been used to generate a large amount of omics data. In the past, single-level analysis has been extensively conducted where the omics measurements at different levels, including mRNA, microRNA, CNV and DNA methylation, are analyzed separately. As the molecular complexity of disease etiology exists at all different levels, integrative analysis offers an effective way to borrow strength across multi-level omics data and can be more powerful than single level analysis. In this article, we focus on reviewing existing multi-omics integration studies by paying special attention to variable selection methods. We first summarize published reviews on integrating multi-level omics data. Next, after a brief overview on variable selection methods, we review existing supervised, semi-supervised and unsupervised integrative analyses within parallel and hierarchical integration studies, respectively. The strength and limitations of the methods are discussed in detail. No existing integration method can dominate the rest. The computation aspects are also investigated. The review concludes with possible limitations and future directions for multi-level omics data integration.

Abstract Multi-omics integration is key to fully understand complex biological processes in an holistic manner. Furthermore, multi-omics combined with new longitudinal experimental design can unreveal dynamic relationships between omics layers and identify key players or interactions in system development or complex phenotypes. However, integration methods have to address various experimental designs and do not guarantee interpretable biological results. The new challenge of multi-omics integration is to solve interpretation and unlock the hidden knowledge within the multi-omics data. In this paper, we go beyond integration and propose a generic approach to face the interpretation problem. From multi-omics longitudinal data, this approach builds and explores hybrid multi-omics networks composed of both inferred and known relationships within and between omics layers. With smart node labelling and propagation analysis, this approach predicts regulation mechanisms and multi-omics functional modules. We applied the method on 3 case studies with various multi-omics designs and identified new multi-layer interactions involved in key biological functions that could not be revealed with single omics analysis. Moreover, we highlighted interplay in the kinetics that could help identify novel biological mechanisms. This method is available as an R package netOmics to readily suit any application.

As terabytes of multi-omics data are being generated, there is an ever-increasing need for methods facilitating the integration and interpretation of such data. Current multi-omics integration methods typically output lists, clusters, or subnetworks of molecules related to an outcome. Even with expert domain knowledge, discerning the biological processes involved is a time-consuming activity. Here we propose PathIntegrate, a method for integrating multi-omics datasets based on pathways, designed to exploit knowledge of biological systems and thus provide interpretable models for such studies. PathIntegrate employs single-sample pathway analysis to transform multi-omics datasets from the molecular to the pathway-level, and applies a predictive single-view or multi-view model to integrate the data. Model outputs include multi-omics pathways ranked by their contribution to the outcome prediction, the contribution of each omics layer, and the importance of each molecule in a pathway. Using semi-synthetic data we demonstrate the benefit of grouping molecules into pathways to detect signals in low signal-to-noise scenarios, as well as the ability of PathIntegrate to precisely identify important pathways at low effect sizes. Finally, using COPD and COVID-19 data we showcase how PathIntegrate enables convenient integration and interpretation of complex high-dimensional multi-omics datasets. PathIntegrate is available as an open-source Python package.

To study complex biological processes holistically, it is imperative to take an integrative approach that combines multi-omics data to highlight the interrelationships of the involved biomolecules and their functions. With the advent of high-throughput techniques and availability of multi-omics data generated from a large set of samples, several promising tools and methods have been developed for data integration and interpretation. In this review, we collected the tools and methods that adopt integrative approach to analyze multiple omics data and summarized their ability to address applications such as disease subtyping, biomarker prediction, and deriving insights into the data. We provide the methodology, use-cases, and limitations of these tools; brief account of multi-omics data repositories and visualization portals; and challenges associated with multi-omics data integration.

Soybean improvement has entered a new era with the advent of multi-omics strategies and bioinformatics innovations, enabling more precise and efficient breeding practices. This comprehensive review examines the application of multi-omics approaches in soybean—encompassing genomics, transcriptomics, proteomics, metabolomics, epigenomics, and phenomics. We first explore pre-breeding and genomic selection as tools that have laid the groundwork for advanced trait improvement. Subsequently, we dig into the specific contributions of each -omics field, highlighting how bioinformatics tools and resources have facilitated the generation and integration of multifaceted data. The review emphasizes the power of integrating multi-omics datasets to elucidate complex traits and drive the development of superior soybean cultivars. Emerging trends, including novel computational techniques and high-throughput technologies, are discussed in the context of their potential to revolutionize soybean breeding. Finally, we address the challenges associated with multi-omics integration and propose future directions to overcome these hurdles, aiming to accelerate the pace of soybean improvement. This review serves as a crucial resource for researchers and breeders seeking to leverage multi-omics strategies for enhanced soybean productivity and resilience.

The recent advent of sophisticated technologies like sequencing and mass spectroscopy platforms combined with artificial intelligence-powered analytic tools has initiated a new era of “big data” research in various complex diseases of still-undetermined cause and mechanisms. The investigation of these diseases was, until recently, limited to traditional in vitro and in vivo biological experimentation, but a clear switch to in silico methodologies is now under way. This review tries to provide a comprehensive assessment of state-of-the-art knowledge on omes, omics and multi-omics in inflammatory bowel disease (IBD). The notion and importance of omes, omics and multi-omics in both health and complex diseases like IBD is introduced, followed by a discussion of the various omics believed to be relevant to IBD pathogenesis, and how multi-omics “big data” can generate new insights translatable into useful clinical tools in IBD such as biomarker identification, prediction of remission and relapse, response to therapy, and precision medicine. The pitfalls and limitations of current IBD multi-omics studies are critically analyzed, revealing that, regardless of the types of omes being analyzed, the majority of current reports are still based on simple associations of descriptive retrospective data from cross-sectional patient cohorts rather than more powerful longitudinally collected prospective datasets. Given this limitation, some suggestions are provided on how IBD multi-omics data may be optimized for greater clinical and therapeutic benefit. The review concludes by forecasting the upcoming incorporation of multi-omics analyses in the routine management of IBD.

The use of multiple omics techniques (i.e., genomics, transcriptomics, proteomics, and metabolomics) is becoming increasingly popular in all facets of life science. Omics techniques provide a more holistic molecular perspective of studied biological systems compared to traditional approaches. However, due to their inherent data differences, integrating multiple omics platforms remains an ongoing challenge for many researchers. As metabolites represent the downstream products of multiple interactions between genes, transcripts, and proteins, metabolomics, the tools and approaches routinely used in this field could assist with the integration of these complex multi-omics data sets. The question is, how? Here we provide some answers (in terms of methods, software tools and databases) along with a variety of recommendations and a list of continuing challenges as identified during a peer session on multi-omics integration that was held at the recent ‘Australian and New Zealand Metabolomics Conference’ (ANZMET 2018) in Auckland, New Zealand (Sept. 2018). We envisage that this document will serve as a guide to metabolomics researchers and other members of the community wishing to perform multi-omics studies. We also believe that these ideas may allow the full promise of integrated multi-omics research and, ultimately, of systems biology to be realized.

Cancer is a complex disease with multiple molecular (omics) factors influencing the risk, development, prognosis, and treatment. Availability of largescale multiple omics data has provided the opportunity to jointly analyse these data using advanced statistical approaches and identify cancer drivers and regulatory pathways underpinning the disease. In the first study, this thesis provides the much-needed guidance for conducting multi-omics analysis using open-source software tools. Next, it introduces an enrichment pipeline developed using imputation-based integration of multi-omics data and was applied to breast and prostate cancers to identify the associated biomarkers and genes.

The role of microbiome in disease onset and in equilibrium is being exposed by a wealth of high-throughput omics methods. All key research directions, e.g., the study of gut microbiome dysbiosis in IBD/IBS, indicate the need for bioinformatics methods that can model the complexity of the microbial communities ecology and unravel its disease-associated perturbations. A most promising direction is the “meta-omics†approach, that allows a profiling based on various biological molecules at the metagenomic scale (e.g., metaproteomics, metametabolomics) as well as different “microbial†omes (eukaryotes and viruses) within a system biology approach. This thesis introduces a bioinformatic framework for microbiota datasets that combines predictive profiling, differential network analysis and meta-omics integration. In detail, the framework identifies biomarkers discriminating amongst clinical phenotypes, through machine learning techniques (Random Forest or SVM) based on a complete Data Analysis Protocol derived by two initiatives funded by FDA: the MicroArray Quality Control-II and Sequencing Quality Control projects. The biomarkers are interpreted in terms of biological networks: the framework provides a setup for networks inference, quantification of networks differences based on the glocal Hamming and Ipsen-Mikhailov (HIM) distance and detection of network communities. The differential analysis of networks allows the study of microbiota structural organization as well as the evolving trajectories of microbial communities associated to the dynamics of the target phenotypes. Moreover, the framework combines a novel similarity network fusion method and machine learning to identify biomarkers from the integration of multiple meta-omics data. The framework implementation requires only standard open source computational biology tools, as a combination of R/Bioconductor and Python functions. In particular, full scripts for meta-omics integration are available in a GitHub repository to ease reuse (https://github.com/AleZandona/INF). The pipeline has been validated on original data from three different clinical datasets. First, the predictive profiling and the network differential analysis have been applied on a pediatric Inflammatory Bowel Disease (IBD) cohort (in faecal vs biopsy environments) and controls, in collaboration with a multidisciplinary team at the Ospedale Pediatrico Bambino Gesú (Rome, I). Then, the meta-omics integration has been tested on a paired bacterial and fungal gut microbiota human IBD datasets from the Gastroenterology Department of the Saint Antoine Hospital (Paris, F), thanks to the collaboration with “Commensals and Probiotics-Host Interactions†team at INRA (Jouy-en-Josas, F). Finally, the framework has been validated on a bacterial-fungal gut microbiota dataset from children affected by Rett syndrome. The different nature of datasets used for validation naturally supports the extension of the framework on different omics datasets. Besides, clinical practice can take advantage of our framework, given the reproducibility and robustness of results, ensured by the adopted Data Analysis Protocol, as well as the biological relevance of the findings, confirmed by the clinical collaborators. Specifically, the omics-based dysbiosis profiles and the inferred biological networks can support the current diagnostic tools to reveal disease-associated perturbations at a much prodromal earlier stage of disease and may be used for disease prevention, diagnosis and prognosis.

The role of microbiome in disease onset and in equilibrium is being exposed by a wealth of high-throughput omics methods. All key research directions, e.g., the study of gut microbiome dysbiosis in IBD/IBS, indicate the need for bioinformatics methods that can model the complexity of the microbial communities ecology and unravel its disease-associated perturbations. A most promising direction is the “meta-omics” approach, that allows a profiling based on various biological molecules at the metagenomic scale (e.g., metaproteomics, metametabolomics) as well as different “microbial” omes (eukaryotes and viruses) within a system biology approach. This thesis introduces a bioinformatic framework for microbiota datasets that combines predictive profiling, differential network analysis and meta-omics integration. In detail, the framework identifies biomarkers discriminating amongst clinical phenotypes, through machine learning techniques (Random Forest or SVM) based on a complete Data Analysis Protocol derived by two initiatives funded by FDA: the MicroArray Quality Control-II and Sequencing Quality Control projects. The biomarkers are interpreted in terms of biological networks: the framework provides a setup for networks inference, quantification of networks differences based on the glocal Hamming and Ipsen-Mikhailov (HIM) distance and detection of network communities. The differential analysis of networks allows the study of microbiota structural organization as well as the evolving trajectories of microbial communities associated to the dynamics of the target phenotypes. Moreover, the framework combines a novel similarity network fusion method and machine learning to identify biomarkers from the integration of multiple meta-omics data. The framework implementation requires only standard open source computational biology tools, as a combination of R/Bioconductor and Python functions. In particular, full scripts for meta-omics integration are available in a GitHub repository to ease reuse (https://github.com/AleZandona/INF). The pipeline has been validated on original data from three different clinical datasets. First, the predictive profiling and the network differential analysis have been applied on a pediatric Inflammatory Bowel Disease (IBD) cohort (in faecal vs biopsy environments) and controls, in collaboration with a multidisciplinary team at the Ospedale Pediatrico Bambino Gesú (Rome, I). Then, the meta-omics integration has been tested on a paired bacterial and fungal gut microbiota human IBD datasets from the Gastroenterology Department of the Saint Antoine Hospital (Paris, F), thanks to the collaboration with “Commensals and Probiotics-Host Interactions” team at INRA (Jouy-en-Josas, F). Finally, the framework has been validated on a bacterial-fungal gut microbiota dataset from children affected by Rett syndrome. The different nature of datasets used for validation naturally supports the extension of the framework on different omics datasets. Besides, clinical practice can take advantage of our framework, given the reproducibility and robustness of results, ensured by the adopted Data Analysis Protocol, as well as the biological relevance of the findings, confirmed by the clinical collaborators. Specifically, the omics-based dysbiosis profiles and the inferred biological networks can support the current diagnostic tools to reveal disease-associated perturbations at a much prodromal earlier stage of disease and may be used for disease prevention, diagnosis and prognosis.

Le tecnologie “-omiche” studiano l’insieme delle molecole presenti nel campione biologico di interesse, in maniera completamente agnostica. L’integrazione di diversi tipi di dati omici, chiamata “multi-omica” o “omica verticale”, fornisce indicazioni importanti su come le cause di una malattia portano alle sue conseguenze funzionali. Queste indicazioni sono particolarmente utili nel caso delle malattie complesse, che sono causate dall’interazione di vari fattori genetici e regolatori con vari contributi ambientali. In questo lavoro, degli approcci multi-omici appropriati sono stati applicati a due malattie complesse che di solito iniziano a manifestarsi durante l’infanzia, hanno un’incidenza crescente, e hanno vari elementi sconosciuti nella loro patologia molecolare, ovvero le malformazioni polmonari congenite e la celiachia. Gli scopi dei due progetti sono, rispettivamente, di verificare se nel tessuto polmonare malformato ci sono varianti genetiche o alterazioni della metilazione del DNA associate al cancro, e di trovare alterazioni comuni nel metiloma e nel trascrittoma di cellule epiteliali dell’intestino tenue di bambini affetti da celiachia. Per quanto riguarda i metodi, nel progetto sulle malformazioni polmonari sono stati usati microarray di metilazione whole genome e sequenziamento dell’intero genoma, mentre nel progetto sulla celiachia sono stati usati microarray di metilazione whole genome e sequenziamento dell’mRNA totale. In tutte le 20 malformazioni polmonari incluse nello studio sono state trovate regioni differenzialmente metilate in geni probabilmente legati al cancro del polmone. Inoltre, 5 campioni malformati avevano almeno una variante somatica missenso in un gene noto come driver del tumore del polmone, e 5 altri campioni avevano un totale di 2 delezioni di oncosoppressori driver del tumore del polmone e 10 amplificazioni di oncogeni driver del tumore del polmone. Questi dati suggeriscono che le malformazioni polmonari congenite possono avere alterazioni genetiche ed epigenetiche di tipo pre-maligno, la cui presenza è impossibile da prevedere sulla base delle sole informazioni cliniche. Nel secondo progetto, una Principal Component Analysis dei dati di metilazione ha mostrato che i pazienti celiaci si dividono in due cluster, di cui uno si sovrappone ai controlli. 174 geni erano differenzialmente metilati rispetto ai controlli in entrambi i cluster. Una Principal Component Analysis dei dati di espressione genica (mRNA-Seq) ha mostrato una distribuzione simile a quella dei dati di metilazione, e 442 geni erano differenzialmente espressi in entrambi i cluster. Sei geni, principalmente coinvolti nella risposta interferonica e nel processo di processamento e presentazione degli antigeni, erano sia differenzialmente espressi che differenzialmente metilati in entrambi i cluster. Questi risultati indicano che le cellule epiteliali dell’intestino tenue di bambini affetti da celiachia sono altamente variabili da un punto di vista molecolare, ma condividono delle differenze fondamentali che le rendono in grado di rispondere agli interferoni e di processare e presentare antigeni con maggiore efficienza rispetto ai controlli. Nonostante le loro limitazioni, gli studi presentati mostrano che degli approcci multi-omici specifici possono essere usati per rispondere alle domande ancora aperte riguardo a diverse malattie, studiando più funzioni cellulari contemporaneamente e spesso portando anche alla generazione di nuove ipotesi e a scoperte inaspettate.
“-Omic” technologies can detect the entirety of the molecules in the biological sample of interest, in a non-targeted and non-biased fashion. The integration of multiple types of omics data, known as “multi-omics” or “vertical omics”, can provide a better understanding of how the cause of disease leads to its functional consequences, which is particularly valuable in the study of complex diseases, that are caused by the interaction of multiple genetic and regulatory factors with contributions from the environment. In the present work appropriate multi-omics approaches are applied to two complex conditions that usually first manifest in childhood, have rising incidence and gaps in the knowledge of their molecular pathology, specifically Congenital Lung Malformations and Coeliac Disease. The aims are, respectively, to verify if cancer-associated genomic variants or DNA methylation features exist in the malformed lung tissue and to find common alterations in the methylome and the transcriptome of small intestine epithelial cells of children with CD. The methods used in the Congenital Lung Malformations project are Whole Genome Methylation microarrays and Whole Genome Sequencing, and for the Coeliac Disease the whole genome methylation microarrays and mRNA sequencing. Differentially methylated regions in possibly cancer-related genes were found in each one of the 20 lung malformation samples included. Moreover, 5 malformed samples had at least one somatic missense single nucleotide variant in genes known as lung cancer drivers, and 5 malformed samples had a total of 2 deletions of lung cancer driver tumour suppressor and 10 amplifications of lung cancer driver oncogenes. The data showed that congenital lung malformations can have premalignant genetic and epigenetic features, that are impossible to predict with clinical information only. In the second project, Principal Component Analysis of the whole genome methylation data showed that CD patients divide into two clusters, one of which overlaps with controls. 174 genes were differentially methylated compared to the controls in both clusters. Principal Component Analysis of gene expression data (mRNA-Seq) showed a distribution that is similar to the methylation data, and 442 genes were differentially expressed in both clusters. Six genes, mainly related to interferon response and antigen processing and presentation, were differentially expressed and methylated in both clusters. These results show that the intestinal epithelial cells of individuals with CD are highly variable from a molecular point of view, but they share some fundamental differences that make them able to respond to interferons, process, and present antigens more efficiently than controls. Despite the limitations of the present studies, they have shown that targeted multi-omics approaches can be set up to answer the relevant disease-specific questions by investigating many cellular functions at once, often generating new hypotheses and making unexpected discoveries in the process.

2015 - 2016
In recent years, the advancement of high-throughput technologies, combined with the constant decrease of the data-storage costs, has led to the production of large amounts of data from different experiments that characterise the same entities of interest. This information may relate to specific aspects of a phenotypic entity (e.g. Gene expression), or can include the comprehensive and parallel measurement of multiple molecular events (e.g., DNA modifications, RNA transcription and protein translation) in the same samples. Exploiting such complex and rich data is needed in the frame of systems biology for building global models able to explain complex phenotypes. For example, theuseofgenome-widedataincancerresearch, fortheidentificationof groups of patients with similar molecular characteristics, has become a standard approach for applications in therapy-response, prognosis-prediction, and drugdevelopment.ÂăMoreover, the integration of gene expression data regarding cell treatment by drugs, and information regarding chemical structure of the drugs allowed scientist to perform more accurate drug repositioning tasks. Unfortunately, there is a big gap between the amount of information and the knowledge in which it is translated. Moreover, there is a huge need of computational methods able to integrate and analyse data to fill this gap. Current researches in this area are following two different integrative methods: one uses the complementary information of different measurements for the 7 i i “Template” — 2017/6/9 — 16:42 — page 8 — #8 i i i i i i study of complex phenotypes on the same samples (multi-view learning); the other tends to infer knowledge about the phenotype of interest by integrating and comparing the experiments relating to it with respect to those of different phenotypes already known through comparative methods (meta-analysis). Meta-analysis can be thought as an integrative study of previous results, usually performed aggregating the summary statistics from different studies. Due to its nature, meta-analysis usually involves homogeneous data. On the other hand, multi-view learning is a more flexible approach that considers the fusion of different data sources to get more stable and reliable estimates. Based on the type of data and the stage of integration, new methodologies have been developed spanning a landscape of techniques comprising graph theory, machine learning and statistics. Depending on the nature of the data and on the statistical problem to address, the integration of heterogeneous data can be performed at different levels: early, intermediate and late. Early integration consists in concatenating data from different views in a single feature space. Intermediate integration consists in transforming all the data sources in a common feature space before combining them. In the late integration methodologies, each view is analysed separately and the results are then combined. The purpose of this thesis is twofold: the former objective is the definition of a data integration methodology for patient sub-typing (MVDA) and the latter is the development of a tool for phenotypic characterisation of nanomaterials (INSIdEnano). In this PhD thesis, I present the methodologies and the results of my research. MVDA is a multi-view methodology that aims to discover new statistically relevant patient sub-classes. Identify patient subtypes of a specific diseases is a challenging task especially in the early diagnosis. This is a crucial point for the treatment, because not allthe patients affected bythe same diseasewill have the same prognosis or need the same drug treatment. This problem is usually solved by using transcriptomic data to identify groups of patients that share the same gene patterns. The main idea underlying this research work is that to combine more omics data for the same patients to obtain a better characterisation of their disease profile. The proposed methodology is a late integration approach i i “Template” — 2017/6/9 — 16:42 — page 9 — #9 i i i i i i based on clustering. It works by evaluating the patient clusters in each single view and then combining the clustering results of all the views by factorising the membership matrices in a late integration manner. The effectiveness and the performance of our method was evaluated on six multi-view cancer datasets related to breast cancer, glioblastoma, prostate and ovarian cancer. The omics data used for the experiment are gene and miRNA expression, RNASeq and miRNASeq, Protein Expression and Copy Number Variation. In all the cases, patient sub-classes with statistical significance were found, identifying novel sub-groups previously not emphasised in literature. The experiments were also conducted by using prior information, as a new view in the integration process, to obtain higher accuracy in patients’ classification. The method outperformed the single view clustering on all the datasets; moreover, it performs better when compared with other multi-view clustering algorithms and, unlike other existing methods, it can quantify the contribution of single views in the results. The method has also shown to be stable when perturbation is applied to the datasets by removing one patient at a time and evaluating the normalized mutual information between all the resulting clusterings. These observations suggest that integration of prior information with genomic features in sub-typing analysis is an effective strategy in identifying disease subgroups. INSIdE nano (Integrated Network of Systems bIology Effects of nanomaterials) is a novel tool for the systematic contextualisation of the effects of engineered nanomaterials (ENMs) in the biomedical context. In the recent years, omics technologies have been increasingly used to thoroughly characterise the ENMs molecular mode of action. It is possible to contextualise the molecular effects of different types of perturbations by comparing their patterns of alterations. While this approach has been successfully used for drug repositioning, it is still missing to date a comprehensive contextualisation of the ENM mode of action. The idea behind the tool is to use analytical strategies to contextualise or position the ENM with the respect to relevant phenotypes that have been studied in literature, (such as diseases, drug treatments, and other chemical exposures) by comparing their patterns of molecular alteration. This could greatly increase the knowledge on the ENM molecular effects and in turn i i “Template” — 2017/6/9 — 16:42 — page 10 — #10 i i i i i i contribute to the definition of relevant pathways of toxicity as well as help in predicting the potential involvement of ENM in pathogenetic events or in novel therapeutic strategies. The main hypothesis is that suggestive patterns of similarity between sets of phenotypes could be an indication of a biological association to be further tested in toxicological or therapeutic frames. Based on the expression signature, associated to each phenotype, the strength of similarity between each pair of perturbations has been evaluated and used to build a large network of phenotypes. To ensure the usability of INSIdE nano, a robust and scalable computational infrastructure has been developed, to scan this large phenotypic network and a web-based effective graphic user interface has been built. Particularly, INSIdE nano was scanned to search for clique sub-networks, quadruplet structures of heterogeneous nodes (a disease, a drug, a chemical and a nanomaterial) completely interconnected by strong patterns of similarity (or anti-similarity). The predictions have been evaluated for a set of known associations between diseases and drugs, based on drug indications in clinical practice, and between diseases and chemical, based on literature-based causal exposure evidence, and focused on the possible involvement of nanomaterials in the most robust cliques. The evaluation of INSIdE nano confirmed that it highlights known disease-drug and disease-chemical connections. Moreover, disease similarities agree with the information based on their clinical features, as well as drugs and chemicals, mirroring their resemblance based on the chemical structure. Altogether, the results suggest that INSIdE nano can also be successfully used to contextualise the molecular effects of ENMs and infer their connections to other better studied phenotypes, speeding up their safety assessment as well as opening new perspectives concerning their usefulness in biomedicine. [edited by author]
L’avanzamento tecnologico delle tecnologie high-throughput, combinato con il costante decremento dei costi di memorizzazione, ha portato alla produzione di grandi quantit`a di dati provenienti da diversi esperimenti che caratterizzano le stesse entit`a di interesse. Queste informazioni possono essere relative a specifici aspetti fenotipici (per esempio l’espressione genica), o possono includere misure globali e parallele di diversi aspetti molecolari (per esempio modifiche del DNA, trascrizione dell’RNA e traduzione delle proteine) negli stessi campioni. Analizzare tali dati complessi `e utile nel campo della systems biology per costruire modelli capaci di spiegare fenotipi complessi. Ad esempio, l’uso di dati genome-wide nella ricerca legata al cancro, per l’identificazione di gruppi di pazienti con caratteristiche molecolari simili, `e diventato un approccio standard per una prognosi precoce piu` accurata e per l’identificazione di terapie specifiche. Inoltre, l’integrazione di dati di espressione genica riguardanti il trattamento di cellule tramite farmaci ha permesso agli scienziati di ottenere accuratezze elevate per il drug repositioning. Purtroppo, esiste un grosso divario tra i dati prodotti, in seguito ai numerosi esperimenti, e l’informazione in cui essi sono tradotti. Quindi la comunit`a scientifica ha una forte necessit`a di metodi computazionali per poter integrare e analizzate tali dati per riempire questo divario. La ricerca nel campo delle analisi multi-view, segue due diversi metodi di analisi integrative: uno usa le informazioni complementari di diverse misure per studiare fenotipi complessi su diversi campioni (multi-view learning); l’altro tende ad inferire conoscenza sul fenotipo di interesse di una entit`a confrontando gli esperimenti ad essi relativi con quelli di altre entit`a fenotipiche gi`a note in letteratura (meta-analisi). La meta-analisi pu`o essere pensata come uno studio comparativo dei risultati identificati in un particolare esperimento, rispetto a quelli di studi precedenti. A causa della sua natura, la meta-analisi solitamente coinvolge dati omogenei. D’altra parte, il multi-view learning `e un approccio piu` flessibile che considera la fusione di diverse sorgenti di dati per ottenere stime piu` stabili e affidabili. In base al tipo di dati e al livello di integrazione, nuove metodologie sono state sviluppate a partire da tecniche basate sulla teoria dei grafi, machine learning e statistica. In base alla natura dei dati e al problema statistico da risolvere, l’integrazione di dati eterogenei pu`o essere effettuata a diversi livelli: early, intermediate e late integration. Le tecniche di early integration consistono nella concatenazione dei dati delle diverse viste in un unico spazio delle feature. Le tecniche di intermediate integration consistono nella trasformazione di tutte le sorgenti dati in un unico spazio comune prima di combinarle. Nelle tecniche di late integration, ogni vista `e analizzata separatamente e i risultati sono poi combinati. Lo scopo di questa tesi `e duplice: il primo obbiettivo `e la definizione di una metodologia di integrazione dati per la sotto-tipizzazione dei pazienti (MVDA) e il secondo `e lo sviluppo di un tool per la caratterizzazione fenotipica dei nanomateriali (INSIdEnano). In questa tesi di dottorato presento le metodologie e i risultati della mia ricerca. MVDA `e una tecnica multi-view con lo scopo di scoprire nuove sotto tipologie di pazienti statisticamente rilevanti. Identificare sottotipi di pazienti per una malattia specifica `e un obbiettivo con alto rilievo nella pratica clinica, soprattutto per la diagnosi precoce delle malattie. Questo problema `e generalmente risolto usando dati di trascrittomica per identificare i gruppi di pazienti che condividono gli stessi pattern di alterazione genica. L’idea principale alla base di questo lavoro di ricerca `e quello di combinare piu` tipologie di dati omici per gli stessi pazienti per ottenere una migliore caratterizzazione del loro profilo. La metodologia proposta `e un approccio di tipo late integration basato sul clustering. Per ogni vista viene effettuato il clustering dei pazienti rappresentato sotto forma di matrici di membership. I risultati di tutte le viste vengono poi combinati tramite una tecnica di fattorizzazione di matrici per ottenere i metacluster finali multi-view. La fattibilit`a e le performance del nostro metodo sono stati valutati su sei dataset multi-view relativi al tumore al seno, glioblastoma, cancro alla prostata e alle ovarie. I dati omici usati per gli esperimenti sono relativi alla espressione dei geni, espressione dei mirna, RNASeq, miRNASeq, espressione delle proteine e della Copy Number Variation. In tutti i dataset sono state identificate sotto-tipologie di pazienti con rilevanza statistica, identificando nuovi sottogruppi precedentemente non noti in letteratura. Ulteriori esperimenti sono stati condotti utilizzando la conoscenza a priori relativa alle macro classi dei pazienti. Tale informazione `e stata considerata come una ulteriore vista nel processo di integrazione per ottenere una accuratezza piu` elevata nella classificazione dei pazienti. Il metodo proposto ha performance migliori degli algoritmi di clustering clussici su tutti i dataset. MVDA ha ottenuto risultati migliori in confronto a altri algoritmi di integrazione di tipo ealry e intermediate integration. Inoltre il metodo `e in grado di calcolare il contributo di ogni singola vista al risultato finale. I risultati mostrano, anche, che il metodo `e stabile in caso di perturbazioni del dataset effettuate rimuovendo un paziente alla volta (leave-one-out). Queste osservazioni suggeriscono che l’integrazione di informazioni a priori e feature genomiche, da utilizzare congiuntamente durante l’analisi, `e una strategia vincente nell’identificazione di sotto-tipologie di malattie. INSIdE nano (Integrated Network of Systems bIology Effects of nanomaterials) `e un tool innovativo per la contestualizzazione sistematica degli effetti delle nanoparticelle (ENMs) in contesti biomedici. Negli ultimi anni, le tecnologie omiche sono state ampiamente applicate per caratterizzare i nanomateriali a livello molecolare. E’ possibile contestualizzare l’effetto a livello molecolare di diversi tipi di perturbazioni confrontando i loro pattern di alterazione genica. Mentre tale approccio `e stato applicato con successo nel campo del drug repositioning, una contestualizzazione estensiva dell’effetto dei nanomateriali sulle cellule `e attualmente mancante. L’idea alla base del tool `e quello di usare strategie comparative di analisi per contestualizzare o posizionare i nanomateriali in confronto a fenotipi rilevanti che sono stati studiati in letteratura (come ad esempio malattie dell’uomo, trattamenti farmacologici o esposizioni a sostanze chimiche) confrontando i loro pattern di alterazione molecolare. Questo potrebbe incrementare la conoscenza dell’effetto molecolare dei nanomateriali e contribuire alla definizione di nuovi pathway tossicologici oppure identificare eventuali coinvolgimenti dei nanomateriali in eventi patologici o in nuove strategie terapeutiche. L’ipotesi alla base `e che l’identificazione di pattern di similarit`a tra insiemi di fenotipi potrebbe essere una indicazione di una associazione biologica che deve essere successivamente testata in ambito tossicologico o terapeutico. Basandosi sulla firma di espressione genica, associata ad ogni fenotipo, la similarit`a tra ogni coppia di perturbazioni `e stata valuta e usata per costruire una grande network di interazione tra fenotipi. Per assicurare l’utilizzo di INSIdE nano, `e stata sviluppata una infrastruttura computazionale robusta e scalabile, allo scopo di analizzare tale network. Inoltre `e stato realizzato un sito web che permettesse agli utenti di interrogare e visualizzare la network in modo semplice ed efficiente. In particolare, INSIdE nano `e stato analizzato cercando tutte le possibili clique di quattro elementi eterogenei (un nanomateriale, un farmaco, una malattia e una sostanza chimica). Una clique `e una sotto network completamente connessa, dove ogni elemento `e collegato con tutti gli altri. Di tutte le clique, sono state considerate come significative solo quelle per le quali le associazioni tra farmaco e malattia e farmaco e sostanze chimiche sono note. Le connessioni note tra farmaci e malattie si basano sul fatto che il farmaco `e prescritto per curare tale malattia. Le connessioni note tra malattia e sostanze chimiche si basano su evidenze presenti in letteratura del fatto che tali sostanze causano la malattia. Il focus `e stato posto sul possibile coinvolgimento dei nanomateriali con le malattie presenti in tali clique. La valutazione di INSIdE nano ha confermato che esso mette in evidenza connessioni note tra malattie e farmaci e tra malattie e sostanze chimiche. Inoltre la similarit`a tra le malattie calcolata in base ai geni `e conforme alle informazioni basate sulle loro informazioni cliniche. Allo stesso modo le similarit`a tra farmaci e sostanze chimiche rispecchiano le loro similarit`a basate sulla struttura chimica. Nell’insieme, i risultati suggeriscono che INSIdE nano pu`o essere usato per contestualizzare l’effetto molecolare dei nanomateriali e inferirne le connessioni rispetto a fenotipi precedentemente studiati in letteratura. Questo metodo permette di velocizzare il processo di valutazione della loro tossicit`a e apre nuove prospettive per il loro utilizzo nella biomedicina. [a cura dell'autore]
XV n.s.

Pathway analysis is an important task for gaining novel insights into the molecular architecture of many complex diseases. With the advancement of new sequencing technologies, a large amount of quantitative gene expression data have been continuously acquired. The springing up omics data sets such as proteomics has facilitated the investigation on disease relevant pathways. Although much work has previously been done to explore the single omics data, little work has been reported using multi-omics data integration, mainly due to methodological and technological limitations. While a single omic data can provide useful information about the underlying biological processes, multi-omics data integration would be much more comprehensive about the cause-effect processes responsible for diseases and their subtypes. This project investigates the combination of miRNAseq, proteomics, and RNAseq data on seven types of muscular dystrophies and control group. These unique multi-omics data sets provide us with the opportunity to identify disease-specific and most relevant biological pathways. We first perform t-test and OVEPUG test separately to define the differential expressed genes in protein and mRNA data sets. In multi-omics data sets, miRNA also plays a significant role in muscle development by regulating their target genes in mRNA dataset. To exploit the relationship between miRNA and gene expression, we consult with the commonly used gene library - Targetscan to collect all paired miRNA-mRNA and miRNA-protein co-expression pairs. Next, by conducting statistical analysis such as Pearson's correlation coefficient or t-test, we measured the biologically expected correlation of each gene with its upstream miRNAs and identify those showing negative correlation between the aforementioned miRNA-mRNA and miRNA-protein pairs. Furthermore, we identify and assess the most relevant disease-specific pathways by inputting the differential expressed genes and negative correlated genes into the gene-set libraries respectively, and further characterize these prioritized marker subsets using IPA (Ingenuity Pathway Analysis) or KEGG. We will then use Fisher method to combine all these p-values derived from separate gene sets into a joint significance test assessing common pathway relevance. In conclusion, we will find all negative correlated paired miRNA-mRNA and miRNA-protein, and identifying several pathophysiological pathways related to muscular dystrophies by gene set enrichment analysis. This novel multi-omics data integration study and subsequent pathway identification will shed new light on pathophysiological processes in muscular dystrophies and improve our understanding on the molecular pathophysiology of muscle disorders, preventing and treating disease, and make people become healthier in the long term.
Master of Science

The uncovering of genes linked to human diseases is a pressing challenge in molecular biology, towards the full achievement of precision medicine. Next-generation technologies provide an unprecedented amount of biological information, but at the same time they unveil enormous numbers of candidate disease genes and pose novel challenges at multiple analytical levels. Multi-omics data integration is currently the principal strategy to prioritise candidate disease genes. In particular, kernel-based methods are a powerful resource for the integration of biological knowledge, but their use is often precluded by their limited scalability. In this thesis, we propose a novel scalable kernel-based method for gene prioritisation which implements a novel multiple kernel learning approach, based on a semi-supervised perspective and on the optimisation of the margin distribution in binary problems. Our method is optimised to cope with strongly unbalanced settings where known disease genes are few and large scale predictions are required. Importantly, it is able to efficiently deal both with a large amount of candidate genes and with an arbitrary number of data sources. Through the simulation of real case studies, we show that our method outperforms a wide range of state-of-the-art methods and has enhanced scalability compared to existing kernel-based approaches for genomic data. We apply the proposed method to investigate the potential role for disease gene prediction of metabolic rearrangements caused by genetic perturbations. To this end, we use constraint-based modelling of metabolism to generate gene-specific information at a genome scale, which is mined via machine learning. Moreover, we compare constraint-based modelling and our kernel-based method as alternative integration strategies for omics data such as transcriptional profiles. Experimental assessments across various cancers demonstrate that information on metabolic rewiring reconstructed in silico can be valuable to prioritise associated genes, although accuracy strongly depends on the cancer type. Despite these fluctuations, predictions achieved starting from metabolic modelling are largely complementary to those from gene expression or pathway annotations, highlighting the potential of this approach to identify novel genes involved in cancer.
La scoperta dei geni legati alle malattie nell'uomo è una sfida pressante in biologia molecolare, in vista del pieno raggiungimento della medicina di precisione. Le tecnologie di nuova generazione forniscono una quantità di informazioni biologiche senza precedenti, ma allo stesso tempo rivelano numeri enormi di geni malattia candidati e pongono nuove sfide a molteplici livelli di analisi. L'integrazione di dati multi-omici è attualmente la strategia principale per prioritizzare geni malattia candidati. In particolare, i metodi basati su kernel sono una potente risorsa per l'integrazione della conoscenza biologica, tuttavia il loro utilizzo è spesso precluso dalla loro limitata scalabilità. In questa tesi, proponiamo un nuovo metodo kernel scalabile per la prioritizzazione di geni, che applica un nuovo approccio di multiple kernel learning basato su una prospettiva semi-supervisionata e sull'ottimizzazione della distribuzione dei margini in problemi binari. Il nostro metodo è ottimizzato per fare fronte a condizioni fortemente sbilanciate in cui si disponga di pochi geni malattia noti e siano richieste predizioni su larga scala. Significativamente, è capace di gestire sia un gran numero di candidati sia un numero arbitrario di sorgenti di informazione. Attraverso la simulazione di casi studio reali, mostriamo che il nostro metodo supera in prestazioni un'ampia gamma di metodi allo stato dell'arte ed è dotato di migliore scalabilità rispetto a metodi kernel esistenti per dati genomici. Applichiamo il metodo proposto per studiare il potenziale ruolo per la predizione di geni malattia dei riarrangiamenti metabolici causati da perturbazioni genetiche. A questo scopo, utilizziamo modelli del metabolismo basati su vincoli per generare informazione sui geni a scala genomica, che viene analizzata tramite apprendimento automatico. Inoltre, compariamo modelli basati su vincoli ed il nostro metodo basato su kernel come strategie di integrazione alternative per dati omici come profili trascrizionali. Valutazioni sperimentali su vari cancri dimostrano come i riarrangiamenti metabolici ricostruiti in silico possano essere utili per prioritizzare i geni associati, nonostante l'accuratezza dipenda fortemente dalla tipologia di cancro. Malgrado queste fluttuazioni, le predizioni basate su modelli metabolici sono largamente complentari a quelle basate su espressione genica o annotazioni di pathway, evidenziando il potenziale di questo approccio per identificare nuovi geni implicati nel cancro.

The advent of advanced high-throughput biological technologies provides opportunities to measure the whole genome at different molecular levels in biological systems, which produces different types of omic data such as genome, epigenome, transcriptome, translatome, proteome, metabolome and interactome. Biological systems are highly dynamic and complex mechanisms which involve not only the within-level functionality but also the between-level regulation. In order to uncover the complexity of biological systems, it is desirable to integrate multi-omic data to transform the multiple level data into biological knowledge about the underlying mechanisms. Due to the heterogeneity and high-dimension of multi-omic data, it is necessary to develop effective and efficient methods for multi-omic data integration. This thesis aims to develop efficient approaches for multi-omic data integration using machine learning methods and network theory. We assume that a biological system can be represented by a network with nodes denoting molecules and edges indicating functional links between molecules, in which multi-omic data can be integrated as attributes of nodes and edges. We propose four network-based approaches for multi-omic data integration using machine learning methods. Firstly, we propose an approach for gene module detection by integrating multi-condition transcriptome data and interactome data using network overlapping module detection method. We apply the approach to study the transcriptome data of human pre-implantation embryos across multiple development stages, and identify several stage-specific dynamic functional modules and genes which provide interesting biological insights. We evaluate the reproducibility of the modules by comparing with some other widely used methods and show that the intra-module genes are significantly overlapped between the different methods. Secondly, we propose an approach for gene module detection by integrating transcriptome, translatome, and interactome data using multilayer network. We apply the approach to study the ribosome profiling data of mTOR perturbed human prostate cancer cells and mine several translation efficiency regulated modules associated with mTOR perturbation. We develop an R package, TERM, for implementation of the proposed approach which offers a useful tool for the research field. Next, we propose an approach for feature selection by integrating transcriptome and interactome data using network-constrained regression. We develop a more efficient network-constrained regression method eGBL. We evaluate its performance in term of variable selection and prediction, and show that eGBL outperforms the other related regression methods. With application on the transcriptome data of human blastocysts, we select several interested genes associated with time-lapse parameters. Finally, we propose an approach for classification by integrating epigenome and transcriptome data using neural networks. We introduce a superlayer neural network (SNN) model which learns DNA methylation and gene expression data parallelly in superlayers but with cross-connections allowing crosstalks between them. We evaluate its performance on human breast cancer classification. The SNN provides superior performances and outperforms several other common machine learning methods. The approaches proposed in this thesis offer effective and efficient solutions for integration of heterogeneous high-dimensional datasets, which can be easily applied to other datasets presenting the similar structures. They are therefore applicable to many fields including but not limited to Bioinformatics and Computer Science.

Les systèmes biologiques sont composés de biomolécules en interaction à différents niveaux moléculaires. D’un côté, les avancées technologiques ont facilité l’obtention des données omiques à ces divers niveaux. De l’autre, de nombreuses questions se posent, pour donner du sens et élucider les interactions importantes dans le flux d’informations complexes porté par cette énorme variété et quantité des données multi-omiques. Les réponses les plus satisfaisantes seront celles qui permettront de dévoiler les mécanismes sous-jacents à la condition biologique d’intérêt. On s’attend souvent à ce que l’intégration de différents types de données omiques permette de mettre en lumière les changements causaux potentiels qui conduisent à un phénotype spécifique ou à des traitements ciblés. Avec les avancées récentes de la science des réseaux, nous avons choisi de traiter ce problème d’intégration en représentant les données omiques à travers les graphes. Dans cette thèse, nous avons développé trois modèles à savoir BraneExp, BraneNet et BraneMF pour l’apprentissage d’intégrations de noeuds à partir de réseaux biologiques multicouches générés à partir de données omiques. Notre objectif est de résoudre divers problèmes complexes liés à l’intégration de données multiomiques, en développant des méthodes expressives et évolutives capables de tirer parti de la riche sémantique structurelle latente des réseaux du monde réel
Biological systems are composed of interacting bio-molecules at different molecular levels. With the advent of high-throughput technologies, omics data at their respective molecular level can be easily obtained. These huge, complex multi-omics data can be useful to provide insights into the flow of information at multiple levels, unraveling the mechanisms underlying the biological condition of interest. Integration of different omics data types is often expected to elucidate potential causative changes that lead to specific phenotypes, or targeted treatments. With the recent advances in network science, we choose to handle this integration issue by representing omics data through networks. In this thesis, we have developed three models, namely BraneExp, BraneNet, and BraneMF, for learning node embeddings from multilayer biological networks generated with omics data. We aim to tackle various challenging problems arising in multi-omics data integration, developing expressive and scalable methods capable of leveraging rich structural semantics of realworld networks