Дисертації з теми "MSPSHA"
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Prater, Gary L. "NPSAT1 Missile System Prelaunch Safety Package (MSPSP)." Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 2004. http://library.nps.navy.mil/uhtbin/hyperion/04Jun%5FPrater.pdf.
Повний текст джерелаArlery, Fabien. "Formes d’ondes MSPSR, traitements et performances associés." Thesis, Evry, Institut national des télécommunications, 2017. http://www.theses.fr/2017TELE0005/document.
Повний текст джерелаNowadays, MSPSR (Multi-Static Primary Surveillance Radar) systems are sustainably settled in air surveillance program [1]. Compared to mono-static radar currently in use, an MSPSR system is based on a sparse network of transmitters (Tx) and receivers (Rx) interconnected to a Central Unit and offers advantages in terms of reliability, cost and performance.Two kinds of MSPSR systems exist: the Passive form and the Active one. While the Passive MSPSR uses transmitters of opportunity such as radio Frequency Modulation (FM) transmitters and/or Digital Video Broadcasting-Terrestrial (DVB-T) transmitters [2], the Active MSPSR uses dedicated transmitters, which emit a waveform that is controlled and designed for a radar application. Each receiver processes the signal coming from all transmitters and reflected on the targets; and the Central Unit restores the target location by intersecting “ellipsoids” from all (transmitter, receiver) pairs. Compared to passive MSPSR, the main advantages of the active MSPSR are the use of dedicated waveforms that allow reaching better performances (like a better association of the transmitters’ contributions at the receiver level); more flexibility in the deployment of transmitters and receivers station (in order to meet the requirements in localisation accuracy and in horizontal and altitude coverages); and the guarantee of having a service continuity. On this purpose, this thesis analyses the differents codes criteria such as the ambiguity function behaviour, the PAPR (Peak to Average Power Ratio), the spectrum efficiency, etc... . Then, in order to find dedicated waveforms for MSPSR systems, one solution is to find easily-constructed families of sequences. Thus building on the works carried out by the Telecommunication field for solving multi-user issues, this document investigates the application of spreading codes and OFDM signals in MSPSR concept. Besides, another solution is to directly generate a set of sequences. Based on cyclic algorithms in [3] we derive a new algorithm that allows to optimize sets of sequences. Similarly, using a gradient descent approach, we develop a more efficient algorithm than the cyclic one. Finally, in order to evaluate the performances of the different algorithms, this thesis generalizes the Levenshtein Bound, establishes new lower bounds on the PSLR (Peak Sidelobe Level Ratio) in mismatched filter case, and studies real data recorded during some trials
Santos, Paula de Souza. "Epitopos imunodominantes da MSP1a de Anaplasma marginale e suas aplicações diagnósticas e vacinais." Universidade Federal de Uberlândia, 2011. https://repositorio.ufu.br/handle/123456789/15730.
Повний текст джерелаAnaplasmose, uma infecção intraeritrocitária obrigatória de bovinos, causada pela Anaplasma marginale, causa severa anemia e alta taxa de aborto, resultando em perda significativa para a indústria de laticínios e carne. O diagnóstico clínico é baseado nos sintomas e exames laboratorias confirmatórios são necessários. Atualmente, todos os ensaios de diagnósticos têm sido desenvolvidos com ELISA indireto de antígenos totais baseado em epítopos múltiplos. Em uma investigação pioneira demonstramos o uso de motivos críticos de um epítopo como biomarcador para a aplicação em imunossensores. Mimotopos do epítopo funcional da proteína MSP1a foram obtidos por meio de Phage Display, após três ciclos de seleção, de uma biblioteca randômica de peptídeos 12-mer contra o anticorpo monoclonal 15D2. Trinta e nove clones foram selecionados aleatoriamente, sequenciados, traduzidos e alinhados com a sequência nativa. Foi obtida a sequência consenso SxSSQSEASTSSQLGA, que está localizada na extremidade C-terminal do motivo repetitivo de 28-aa da proteína MSP1a, mas o alinhamento e a variação das sequências entre os mimotopos nos permitiu mapear o motivo crítico STSSxL dentro da sequência consenso. Com base nesses resultados, dois peptídeos foram quimicamente sintetizados; um baseado no motivo crítico (STSSQL, Am1) e o outro baseado na sequência consenso alinhada com o epítopo nativo (SEASTSSQLGA, Am2). Soro de 24 animais infectados e 52 animais saudáveis foram testados por ELISA quanto à reatividade contra Am1 e Am2, que apresentou sensibilidades de 96% e 100%, respectivamente. O peptide Am1 foi incorporado a um bioeletrodo (grafite modificado com ácido poli-3-hidroxyfenilacético) e a detecção direta de soro foi demonstrada por impedância, voltametria de pulso diferencial e microscopia de força atômica. O sistema de sensor eletroquímico provou ser altamente eficaz em soro de animais positivos de negativos. Este imunossensor foi altamente sensível e seletivo para IgG positiva, contaminantes não afetaram as medições, e foram baseados e um sistema simples, rápido e reprodutível.
Doutor em Genética e Bioquímica
Orner, Sherko A. "Functional characterisation of Neisseria meningitidis autotransporters MSPA and APP." Thesis, University of Nottingham, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.490049.
Повний текст джерелаGaja, Tomáš. "Návrh anténní řady pro MSPS radar pracující v pásmu L." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2017. http://www.nusl.cz/ntk/nusl-316426.
Повний текст джерелаEkbäck, Maria Palmetun. "Hirsutism and quality of life with aspects on social support, anxiety and depression." Doctoral thesis, Örebro universitet, Institutionen för hälsovetenskap och medicin, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-32020.
Повний текст джерелаRicci, Luca. "Design of a 12-bit 200-MSps SAR Analog-to-Digital converter." Thesis, KTH, Skolan för elektroteknik och datavetenskap (EECS), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-284559.
Повний текст джерелаSAR (Analog-Digital-omvandlaren) är en av de mest energieffektiva omvandlare. I den här avhandlingen är utvecklingen av en SAR ADC i en 28-nm CMOS-teknik baserad på laddning omfördelning presen- teras.Den implementerade SAR ADC använder en omkopplingsprocedur baserad på en modifierad version av den monotoniska omkopplingsalgoritm för att reducera omkopplingsenergin och DAC-området. DAC är en binärviktad matris med enhetskondensatorer. En anpassad kondensator för enheten har utformats med ett värde av 0,8 fF för att minska DAC-energiförbrukningen. Två komparatorer har implementerats, en dynamisk komparator och en statisk komparator. Den dynamiska implementering gör det möjligt att få bättre prestanda. Därför väljs den dynamiska komparatorn SAR ADC. Provtagningsomkopplarna startas upp för att minska icke-lineariteten introduceras när insignalen samplas. SAR-operationerna styrs av en asynkron logik implementerad som en beteendemodell i Verilog-A.Effekten av de designade kretsarna på konverterarens linearitet bedöms med integralen icke-linearitet (INL) och differentiell icke-linearitet (DNL). Dessutom är ADC: s prestanda bedömdes i termer av signal-till-brus-och-distorsionsförhållande (SNDR). Samsimulering av Verilog-A beteendemodeller och scheman tillåts utvärdera effekten av varje block på prestandan hos ADC. Omvandlaren kan uppnå en ENOB på 10,9 med en samplingshastighet på 200 MSps, vilket resulterar i en FoM eller 7,4 fJ / konv.- steg.
Huff, Jason. "Functional determinants of the porin MspA and its role in permeabilizing mycobacterial outer membranes." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2010. https://www.mhsl.uab.edu/dt/2010p/huff.pdf.
Повний текст джерелаKhairalla, Ahmed Samir Mohammed. "Identification of host immune cell receptors mediating the binding of meningococcal App/MspA proteins." Thesis, University of Nottingham, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.575478.
Повний текст джерелаLesniewska, Karolina. "New tools reveal interaction determinants and post-mitotic function of crucial microtubule regulators." Thesis, University of Edinburgh, 2014. http://hdl.handle.net/1842/9371.
Повний текст джерелаPark, Dae Soon. "A comparative assessment of modal shift policies (MSPs) in the passenger transport sector in Korea." Thesis, University of Southampton, 2016. https://eprints.soton.ac.uk/388075/.
Повний текст джерелаKozuki, Haruka. "Trait-Based Subtypes of ASD by the Multi-Dimensional Scale for PDD and ADHD (MSPA)." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/264654.
Повний текст джерелаAraújo, Fábio Ribeiro de. "Avaliação das proteínas recombinantes msp1a e msp2 de anaplasma marginale, associadas a oligonucleotídeo cpg 2006 como adjuvante, como imunógenos contra a anaplasmose bovina." Instituto de Ciências da Saúde, 2005. http://repositorio.ufba.br/ri/handle/ri/20179.
Повний текст джерелаMade available in DSpace on 2016-08-29T17:59:17Z (GMT). No. of bitstreams: 1 Tese_ICS_ Flábio Ribeiro de Araújo.pdf: 1589352 bytes, checksum: eef290e5cf5f5ad0b3942824ce7c94fd (MD5)
FUNDECT;CNPq
A anaplasmose é uma importante enfermidade de bovinos de áreas tropicais e subtropicais do mundo, causada pela riquétsia intra-eritrocítica Anaplasma marginale. A vacinação tem sido a forma mais econômica e eficiente de controlar a anaplasmose bovina. No entanto, os métodos de imunização tradicionais, que utilizam organismos provenientes de eritrócitos infectados, apresentam limitações em seu uso, razão pela qual, estudos para o descobrimento de novos imunógenos são necessários. Nos últimos anos, esses estudos têm se concentrado nas proteínas de membrana da riquétsia, sobretudo MSP1a e MSP2, as quais mostraram-se promissoras em ensaios de proteção com as proteínas nativas, utilizadas isoladamente. Este trabalho teve como objetivo geral testar a imunoproteção induzida pelas proteínas de membrana MSP1a e MSP2 recombinantes de A. marginale, associadas com adjuvante CpG ODN 2006, frente a desafio heterólogo e avaliar a resposta imune gerada pela imunização. Para tanto, diversas etapas experimentais foram executadas, desde a clonagem e expressão dos genes que codificam as duas proteínas, padronização dos testes de diagnóstico usados no estudo, imunização e desafio dos animais experimentais e acompanhamento das respostas imunes humoral e celular. Os ELISAS indiretos baseados em MSP1a e MSP2 recombinantes apresentaram sensibilidades de 99,0% e especificidades de 100% (para ambos os testes). A PCR para o gene msp5 da riquétsia detectou infecções ainda no período pré-patente, antes da conversão sorológica. Os bovinos da raça Aberdeen Angus foram imunizados três vezes com 200 μg de MSP2 e/ou MSP1a recombinantes (produzidas a partir do isolado Pernambuco – Zona da Mata), associadas com CpG ODN 2006 e alúmen. Posteriormente, foram desafiados com 3 x 107 eritrócitos infectados do isolado heterólogo de A. marginale (Mossoró - Rio Grande do Norte). Os animais experimentais apresentaram quadro clínico de anaplasmose (redução do volume globular, febre e riquetsemias detectáveis por distensões sangüíneas coradas). Não foram detectadas diferenças significativas entre os grupos imunizados e os controles (que receberam CpG ODN 2006 e alúmen ou alúmen isoladamente) quanto ao percentual de redução do volume globular, riquetsemias máximas e temperaturas retais máximas, indicando que as imunizações não foram protetoras. A despeito da significativa produção de IgG total contra MSP1a e MSP2, detectada no dia do desafio, os animais imunizados apresentaram produção significativa de 14 IgG2 apenas contra MSP1a. Não foi possível detectar resposta proliferativa de células mononucleares de sangue periférico, tanto no momento do desafio quanto após o pico das riquetsemias. Possivelmente, a ausência de proteção deveu-se a uma deficiente ou ausente estimulação de imunidade celular nos bovinos imunizados.
Basel, Matthew T. "Targeting cancer therapy: using protease cleavage sequences to develop more selective and effective cancer treatments." Diss., Kansas State University, 2010. http://hdl.handle.net/2097/14087.
Повний текст джерелаDepartment of Chemistry
Stefan H. Bossmann
This paper describes two methods for utilizing cancer associated proteases for targeting cancer therapy to the tumor. The first method is designing a drug delivery system based on liposomes that are sensitive to cancer associated proteases. Upon contact with the protease, the liposome releases its contents. The second method is designing a prodrug that is based on a porin isolated from Mycobacterium smegmatis. The porin is modified with protease consensus sequences, inhibiting its toxicity. Upon contact with the protease, the drug is activated. Protease sensitive liposomes were synthesized that were sensitive to urokinase plasminogen activator. This was done by synthesizing a cholesterol-anchored, uPA consensus – sequence-containing, acrylic acid block copolymer and using it to form a covalently bound polymer cage around the outside of a hypertonic liposome. Liposomes were synthesized that had a diameter of 136 nm. Upon addition of the polymer the diameter increased by 2.69 nm, indicating it had successfully embedded into the liposome membrane. After crosslinking with either a short peptide containing a lysine (so that it is a diamine) or ethylenediamine, the diameter increased between 5.33 nm and 14.1 nm (depending on the type and amount of the crosslinked). Fluorescence release assays showed that the polymer cage could add in excess of thirty atmospheres of osmotic pressure resistance, and, under isobaric conditions, would prevent release of much of the liposomal contents. Upon treatment with uPA, the polymer caged liposomes released a significantly larger amount of their contents making the liposomes protease sensitive. MspA was shown to be a very stable protein able to be imaged by AFM. AFM imaging demonstrated that MspA is able to form native pore structures in membranes making it a good imitator of the membrane attack complex. MspA was demonstrated to be highly cytotoxic, but poor at distinguishing between cells. Pro-MspA was synthesized by adding a hydrophilic peptide to MspA that prevents insertion. A uPA cleavage sequence embedded causes the MspA to become activated at the cancer site. This was demonstrated in tests against uPA and non-uPA producing cell lines.
Perera, Jayaweeralage Ayomi Sheamilka. "Investigation of stability, dynamics and scope of application of mycobacterial porin MspA: a highly versatile biomolecular resource." Diss., Kansas State University, 2012. http://hdl.handle.net/2097/14845.
Повний текст джерелаDepartment of Chemistry
Stefan H. Bossmann
Porin A from Mycobacterial smegmatis (MspA) is an octameric trans-membrane channel protein and is one of the most stable porins known to date. MspA has been successfully isolated and purified to obtain liquid extracts and crystals using a modified extraction procedure. A full analytical assessment has been carried out to authenticate its’ structure, including gel electrophoresis, spectroscopy (fluorescence, UV, FTIR, NMR), HPLC, Bradford protein assay, dynamic light scattering and X-ray crystallography. Nanoscopic vesicle formation of MspA molecules in aqueous media has been thoughroughly investigated. Temperature dependent dynamic light scattering experiments reveal that size of such vesicles is dependent on temperature but is independent of ionic strength of the medium. Zeta potential measurements reveal a steady build up of positive charge on the vesicle surface with increasing temperature. For the first time, wild type (WT) MspA has been utilized as a channel forming agent. This phenomenon has future potential in DNA sequencing and the development of antimycobacterial drugs. Channel activity of WT MspA and mutant A96C MspA has been investigated and has shown to form stable channels across DPhPC lipid bilayers. Blocking of the channel current via external molecules (i.e. channel blocking) is an extremely important process, which helps to evaluate the biosensor ability of the pore. In this regard, two Ruthenium based compounds, Ru(QP-C2)38+ (i.e. RuC2) and Ru(bpy)32+have been successfully employed as channel blocking agents. Both compounds show evidence for channel blocking of WT MspA. However, these results are not reproducible. Three dimensional aggregation behavior of RuC2-MspA vesicles have been thoughroughly investigated. It is evident that addition of RuC2 significantly increases vesicle size and polydispersity of MspA aggregates in solution. The results provide explanations onto the lack of channel blocking ability of MspA by RuC2. Development of a ‘greener’ dye sensitized solar cell with the use of MspA as an electron carrier is investigated for the first time. A series of Ru(II)-phenanthroline-based dyes have been synthesized as non-toxic dyes in this regard. Chemical binding between the dyes and MspA has been achieved successfully. Two types of solar cell prototypes, i.e. TiO2-based (Grätzel type) and FTO-based have been developed and tested. Significant current generation and conversion efficiencies have been achieved for both cell types. This marks the first development of a protein-based photovoltaic device, which has the potential to be developed as a new class of “hybrid soft solar cells”.
Shrestha, Tej Bahadur. "Heterocycles for life-sciences applications and information storage." Diss., Kansas State University, 2010. http://hdl.handle.net/2097/13540.
Повний текст джерелаDepartment of Chemistry
Stefan H. Bossmann
The photochromic spirodihydroindolizine/betaine (DHI/B) system has been reinvestigated applying picosecond, microsecond, stationary absorption measurements, and NMR-kinetics. The first surprise was that the electronic structure of the betaines is quite different than commonly assumed. The photochemical ring-opening of DHIs to betaines is a conrotatory 1,5 electrocyclic reaction, as picosecond absorption spectroscopy confirms. The (disrotatory) thermal ring-closing occurs from the cisoid betaine. The lifetime of the transoid betaine is 60 s at 300 K, whereas the lifetime of the cisoid isomer is of the order of 250 microseconds. According to these results, the electrocyclic back reaction of the betaines to the DHI is NOT rate determining, as previously thought, but the cisoid-transoid-isomerization of the betaine. Although the presence of a second nitrogen atom increases the photostability of the spirodihydroindolizine-pyridazine/betaine-system remarkably, the photochemical reaction mechanism appears to be exactly the same for spirodihydroindolizine-pyridazine/betaine-system. A nondestructive photoswitch or an information recording systems has been explored using styryl-quinolyldihydroindolizines. Both isomers DHI and betaine are fluorescent. When the blue betaine is stabilized in a thin polymethyl methacrylate (PMMA) matrix, it is stable for several hours even in room temperature and very stable at 77K. Although irradiation of visible light = 532 nm allows the photo-induced reaction of the Betaine back to the DHI, a nondestructive read-out can be performed at λ = 645 nm upon excitation with λ = 580 nm. Image recording (write) and read-out, as well as information storage (at 77K) have been demonstrated. Charged and maleimide-functionalized DHI/B systems have beed synthesized for use as photochemical gates of the mycobacterial channel porin MspA. Positively charged and maleimide functionalized DHI groups that were attached to the DHI/B-system permit the binding of the photoswitch to selective positions in the channel proteins due to the presence of a cysteine moiety. An inexpensive new method for the large scale synthesis of coelenterazine is developed. A modified Negishi coupling reaction is used to make pyrazine intermediates from aminopyrazine as an economical starting material. This method permits the use of up to 1g coelenterazine per kg body weight and day, which turns the renilla transfected stem cells into powerful light sources.
Kano, Flora Satiko. "Anaplasma marginale : análise da variabilidade do gene msp1 a e avaliação imunogênica da vacina de DNA contendo genes para MSP1a, MSP1b e MSP5 em camundongos BALB/c." Universidade Estadual de Londrina. Centro de Ciências Agrárias. Programa de Pós-Graduação em Ciência Animal, 2007. http://www.bibliotecadigital.uel.br/document/?code=vtls000123331.
Повний текст джерелаAnaplasma marginale is an important tick-transmitted rickettsial pathogen of cattle that invades and multiplies within erythrocytes, causing severe hemolytic anemia during acute infection. Immunization with purified outer membrane proteins (OMP) induces protection against acute A. marginale disease. Of 21 OMP described six major surface proteins (MSPs 1a, 1b, 2-5) have been well-characterized. The complex MSP1 (MSP1a and 1b) is an adhesin for bovine erythrocyte, and MSP1a vary in size and sequence due to the number of tandem 28-29-amino acid repeats. DNA vaccine against anaplasmosis has been investigated using the msp1a and msp1? genes, and the results related cellular and humoral response in mice and cattle. Immunization with DNA plasmids encoding antigens of interest represents a novel and promising method in vaccine research and development. Multiepitope DNA vaccine is a new experience to increase immunogenicity and protection for vaccinated animal as compared in single epitope. The objectives of this study were to analyze the variability of the msp1a gene of A. marginale strains from Parana State, evaluate the capacity of the recombinant plasmids to express MSP1a, MSP1b, and MSP5 in eukaryotic cells, and evaluate the immunogenicity of BALB/c mice immunized with these DNA vaccines encoding MSPs of A. marginale PR1 strain individually or in association. The analysis of the msp1a gene identified the presence of six, five and three tandem repeats in PR1, PR2 PR3, respectively; however, the region of MSP1a responsible for immunogenicity was conserved. The plasmids pcDNA-msp1?, pcDNA-msp1? and pcDNA-msp5, which encode the MSPs genes under the control of cytomegalovirus enhancer/promoter and intron A, were constructed, multiplied in TOP10 E. coli and purified. Expression of MSP1a, MSP1b, and MSP5 in vitro was performed into Vero cells using lipofectamine 2000, following Indirect Immunofluorescen Assay (IFA) using monoclonal antibodies. Seven experimental groups of mice were immunized to evaluate the production of whole IgG and to determinate IgG1 and IgG2a isotype: G1-100 ml PBS; G2-100 mg empty vector; G3-100 mg A. marginale initial bodies + Freund´s adjuvant; G4-100 mg pcDNA-msp1a; G5-100 mg pcDNA-msp1b; G6-100 mg pcDNA-msp5; and G7-pool of recombinant plasmids (33 mg for each). Three weeks after the last immunization, mice were sacrified to evaluate spleen cells proliferation. Vero cells transfected with recombinants plasmids reacted with specific monoclonal antibodies, demonstrating the expression of msp genes. Specific IgG against MSP1a and MSP5 were detected in either by ELISA and Western blot. The groups that received pcDNA-msp1a and pcDNA-msp5 exhibited predominance for IgG2a production and splenocytes proliferation, suggesting that these recombinant plasmids are good candidates for elicited T helper 1 immune response. The association of three recombinant plasmids (pcDNA-msp1a, pcDNA-msp1b and pcDNA-msp5) used in the immunization of mice induced high antibodies response by ELISA and reacted with all recombinant proteins (rMSP1a, rMSP1b, and rMSP5) of A. marginale by Western blot. Also, the combination of plasmids provide strong lymphoproliferation (SI = 12,2), whereas the genes to MSP1a provide significant splenocytes (SI = 2,6) and the genes to MSP1b and MSP5 did not provide significant proliferation (SI<2). The results showed no suppression when the recombinant plasmids were taken in association, and demonstrated that they can generate significant T-cell lymphocyte. Thus, the immunization in association of recombinant plasmids encoding MSPs can be an effective strategy for immunoprofilaxy of anaplasmosis.
Rajnoha, Peter. "Analýza jader real-time operačních systémů běžících na platformě FITkit." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2009. http://www.nusl.cz/ntk/nusl-236754.
Повний текст джерелаLamrabet, Otmane. "Modifications génétiques de Mycobacterium tuberculosis : interactions avec les organismes hôtes." Thesis, Aix-Marseille, 2012. http://www.theses.fr/2012AIXM5027/document.
Повний текст джерелаMycobacteria are mycolic-acid containing, high GC% bacterial organisms which can be recovered from soil and fresh water environments where free-living protozoa also live. Co-isolation of mycobacteria and amoeba collected from such environmental sources has been reported. Several experiments further demonstrated the ability of most environmental mycobacteria to survive in the amoebal trophozoites and cysts and in some eukaryotic cells including macrophages. Genetic modification of mycobacteria in general and mycobacteria belonging to Mycobacterium tuberculosis complex in particular are complicated and no studies using genetic modification of mycobacteria (pathogenic or non-pathogenic) had been performed in our laboratory prior to our work. In our thesis work, we showed that amoebae or other phagocytic organisms can serve as sources and places for gene transfers in mycobacteria. Gene transfers may have contributed to the adaptation of mycobacteria to an intracellular lifestyle. In addition, we developed two co-culture systems: Mycobacterium smegmatis-Acanthamoeba polyphaga and Mycobacterium gilvum-A. polyphaga and we clarified the spectrum of rapid-growing mycobacteria and amoeba interactions. This model of mycobacteria-amoeba interactions was then used to test another hypothesis according to which unlike the prevailing paradigm, the addition of genes does not reduce the virulence of bacteria. For the first time in our laboratory we modified two species of the M. tuberculosis complex, M. tuberculosis H37Rv and Mycobacterium bovis BCG to observe the effect of these changes on their pathogenicity and survival
Siyambalagoda, Gamage Pubudu Hasanka. "Synthesis and applications of ruthenium(II)quaterpyridinium complexes and Poly-N-isopropylacrylamide/ acrylic acid copolymers." Diss., Kansas State University, 2009. http://hdl.handle.net/2097/4621.
Повний текст джерелаDepartment of Chemistry
Stefan Bossmann
Tris-homoleptic ruthenium(II)-quaterpyridyl and quaterpyridinium complexes, with +8 and +14 charge were synthesized by utilizing high pressure reaction pathway. These complexes have diameters ranging from 1.82 to 4.55 nm according to the molecular modeling calculations. These ruthenium complexes are highly luminescent and contain long excited state life times. The novel ruthenium(II)-quaterpyridinium complexes exhibit superior reactivity as sensitizer-relay-assemblies (SRA‟s) in sacrificial systems for water and carbon dioxide reductions, while harvesting the ultraviolet- and most of the visible fraction of the incident solar spectrum. Ru(II)-quaterpyridinium complexes and Pd/TiO2 catalysts were successfully used as the catalytic system for the photo catalytic reduction of water and carbon dioxide to hydrogen and methane respectively. Phosphonate-tethered Ru(II)-quaterpyridinium complexes were synthesized from Ru(II)-tris-quaterpyridyl complexes. These complexes form stable adhesive layers on indium tin oxide (ITO) electrodes. A series of differential pulse voltammetry experiments were carried out to measure the ground state and excited state redox potentials of all the Ru(II)quaterpyridinium complexes. The reductive potentials obtained were compared with the reductive potentials of CO2 to CH4 and H2O to H2 reductions. The measurements obtained from the experiments confirmed that it is possible to thermodynamically oxidize water and reduce CO2 by using phosphonate-tethered Ru(II)-quaterpyridinium complexes. These complexes are successfully utilized as prototypes for mycobacterial channel blockers. The Ru(II) complexes show distinct changes in their luminescence spectra when bound to the porin MspA from M. smegmatis, which is a non-pathogenic relative of M. tuberculosis. By using HPLC, we have determined binding constants of the Ru(II)-complexes to MspA in phosphate buffer (0.05 M, pH = 6.8) ranging from 5.2 x 109 M-1 (Ru-C2) to 1.8 x 109 M-1 (Ru-C4). Our findings indicate that channel blocking is a promising treatment strategy for mycobacterial infections. Poly-N-isopropyl-acrylamide/acetic acid copolymers were synthesized and characterized by elemental analysis and gel permeation chromatography. The average composition of the copolymers determined from CHN analysis is in excellent correlation with the feed composition indicating that the radical polymerization process is indeed statistical. Crosslinking of individual polymer chains permitted the generation of ultraflat layers on Mica surfaces by a simple spin-casting procedure, which are able to host the mycobacterial channel protein MspA, while retaining its channel function.
Omar, Omar Jaber. "An On-Chip Memory for Testing of High-Speed Mixed-Signal Circuits." Thesis, Linköpings universitet, Elektroniska komponenter, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-103800.
Повний текст джерелаSenate, University of Arizona Faculty. "Faculty Senate Minutes April 7, 2014." University of Arizona Faculty Senate (Tucson, AZ), 2014. http://hdl.handle.net/10150/316677.
Повний текст джерелаDiop, Mayoro. "De l'approche monétaire à l'approche par les capabilités : une analyse multidimensionnelle de la pauvreté au Sénégal sur les données de l'Enquête de Suivi de la Pauvreté au Sénégal (ESPS2)." Thesis, Reims, 2014. http://www.theses.fr/2014REIME003.
Повний текст джерелаThe aim of this thesis is to propose a multidimensional analysis of poverty in Senegal, and to show the importance of integrating the capability approach in the analysis of poverty. This thesis aims at showing that the capability approach is an adequate and appropriate framework for identifying the poor and proves to be of real interest in setting up better public policies in order to fight against poverty relating to the MDGs.This study is divided in two parts. The first part deals with the concept of poverty confronting the traditional monetary approach with the capability approach, and shows the interest of the economics of happiness in the analysis of poverty and well being. In a second phase, the objective is to illuminate methodological options in order to treat the extent of poverty empirically and bring the arguments in favor of the capability approach.The essential features of this thesis revolve around three main contributions. The first one is theoretical. It offers an analysis of poverty in Senegal in terms of capabilities. The second is empirical and provides a multidimensional measure based on the theory of fuzzy sets from the monitoring survey of poverty in Senegal (MSPS 2). The third one is methodological and proposes an original approach consisting of building a core of utilitarian poverty (by comparing the monetary and subjective poverty) and then analyzing the evolution of the core according to the distribution of the degrees of poverty capabilities
Niebler, Sandra. "Funktionalisierung von Goldelektroden mit organischen Monoschichten und Kanalproteinen (MspA) /." 2005. http://www.gbv.de/dms/bs/toc/49026249X.pdf.
Повний текст джерелаGandara, Miguel Francisco. "A 12-bit, 10 Msps two stage SAR-based pipeline ADC." 2012. http://hdl.handle.net/2152/19973.
Повний текст джерелаtext
Liu, chia-chun, and 劉家鈞. "A 10-bit 250-MSPS Digital to Analog Converter for WLAN Applications." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/56263521648757433594.
Повний текст джерела中華大學
電機工程學系(所)
94
This thesis presents a 10-bit 250-MSPS 1.8-V digital to analog converter (DAC) and is implemented in TSMC 0.18μm CMOS technology. A segmented current steering architecture is used with optimized performance for speed, resolution, power consumption and area. The DAC can be operated up to 250MHz sampling frequency and the settling time is less than 3.5 ns. The differential nonlinearity (DNL) and integral nonlinearity (INL) are ±0.15 and ±0.13 least significant bits (LSBs), respectively. The spurious free dynamic range (SFDR) at 250-MSPS remains above 63.6 dB for input frequency up to 120 MHz. Total power dissipation is 30.6 mW with 1.8-V power supply. The chip size is 1.258 mm × 1.485 mm.
Hwang, Shan-Chun, and 黃善君. "The Design and Realization of Digital Calibration in 10-bit 10 MSPS Pipelined ADC." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/59693826365920488412.
Повний текст джерела國立臺灣大學
電機工程學研究所
89
The goal of this thesis is to understand the calibration in capacitor’s mismatch error for pipelined ADC, one precision multiply-by-2 circuit is made from Switch-Capacitor, the capacitor’s mismatch error will reduce the Differential Nonlinearity(DNL), for increasing the pipelined ADC’s Signal-to-Noise ratio, we first have to understand the calibration algorithm in capacitor’s mismatch. This thesis is divided six chapters, chapter 1 and chapter 2 is the introduction and the classification in ADC. In chapter 5 is the layout and test , chapter 6 is the conclusion. The followings are chapter 3 and 4’s brief description: In chapter 3, the calibration method is divided two domain, analog-domain and digital-domain. The analog-domain used in calibration for pipelined ADC are larger in area and complex in design. If we use the digital-domain, the area will be smaller and the design is simpler. In this thesis, the improvement in SNR is about 1.5~2 bit under 1% capacitor error mismatch. In chapter 4, we described the detailed circuit buliding block in pipelined ADC, containing operational amplifier, multipling DAC, fully differential comparator, and sample and hold circuit. The operation speed in pipelined ADC is the design in opamp. and MDAC, we described some design issues in the operational amplifier.
Hillmann, Dietmar [Verfasser]. "Expression and regulation of the porin gene mspA of Mycobacterium smegmatis / vorgelegt von Dietmar Hillmann." 2007. http://d-nb.info/982920245/34.
Повний текст джерелаZhang, Li-Lun, and 張立侖. "Design and Implementation of a Low Power, 10-Bit, 20 MSPS Pipeline A/D Converter." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/91161074286118140200.
Повний текст джерела國立成功大學
電機工程研究所
84
In this thesis, a differential low power, 10-bit, 20 MSample/ sec (MSPS) pipeline A/D converter for vedio- rate applications is designed. The 1.5b/stage pipeline architecture with digital correction is adopted in the framework. The whole-chip simulation shows 52 dB signal-to-(noise+distortion) ratio (SNDR) for 1.25MHz input sampled at 20 MSPS. The prototype A/D converter has been fabricated in a double-poly-double-metal 0.6-um CMOS technology.
Hoffmann, Christian [Verfasser]. "Strategies for cryo-electron tomography of the mycobacterial cell envelope and its pore proteins and functional studies of porin MspA from Mycobacterium smegmatis / Christian Werner Hoffmann." 2010. http://d-nb.info/1006985409/34.
Повний текст джерела