Дисертації з теми "Monooxygenase oxidation"
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Sowden, Rebecca. "The oxidation of terpenoid hydrocarbons by monooxygenase enzymes." Thesis, University of Oxford, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.288518.
Повний текст джерелаCharlton, Susan. "The particulate form of the enzyme methane monooxygenase." Thesis, University of Warwick, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323371.
Повний текст джерелаMaurer, Steffen Christian. "Oxidationsreaktionen mittels der Cytochrom P450-Monooxygenase CYP102A1 in Enzymreaktoren." [S.l. : s.n.], 2006. http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-28118.
Повний текст джерелаHogan, Matthew Charles. "Process issues in redox biocatalysis : cyclohexanone monooxygenase catalysed chiral lactone syntheses." Thesis, University College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325655.
Повний текст джерелаPilkington, Simon John. "The soluble methane monooxygenase and ammonia oxidation in the obligate methanotroph 'Methylosinus trichosporium (OB3b)'." Thesis, University of Warwick, 1986. http://wrap.warwick.ac.uk/66751/.
Повний текст джерелаKeppler, Artur Franz. "Biotransformações de cetonas aromáticas e cíclicas promovidas por fungos." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/46/46135/tde-13032007-123741/.
Повний текст джерелаIn this work, we evaluated the enzymatic potential of different Aspergillus strains, through the biotransformations of two substrates: 2- and 4-methylcyclohexanone (1a e 1b). All the strains employed showed alcohol dehydrogenase and Baeyer-Villiger monooxygenase (CPMO and CHMO) activities. These enzymes can perform ketone biorreduction and oxidation. Using the A. terreus SSP 1498 selected from the screening study, we prepared alcohols and lactones in good enantiosselectivity. In this way, other fungal strains were studied aiming to determine the presence of monooxygenase activity by means of the biotransformation of aromatic ketones. Like the Aspergillus, we observed that all strains used in this study showed alcohol dehydrogenase and Baeyer-Villiger monooxygenase (APMO) activities. We selected 1-phenyl-etanone and its para substituted derivates as substrates. Additionally, we synthesized eight examples of bi-functionalized compounds with sulfide, selenide and ketone groups. These compounds were submitted to the action of enzymatic system of different fungi which were selected from the initial screening. The products from the biotransformation were isolated and characterized.
Valentine, Ann M. (Ann Margaret) 1971. "Bioinorganic hydrocarbon oxidation : mechanistic and kinetic studies of the soluble methane monooxygenase from Methylococcus capsulates (bath)." Thesis, Massachusetts Institute of Technology, 1998. http://hdl.handle.net/1721.1/50508.
Повний текст джерелаIncludes bibliographical references (p. 219-233).
Chapter 1. Principles of Small Molecule Activation by Metalloproteins as Exemplified by the Soluble Methane Monooxygenase -- Chapter 2. Small Molecule Binding to the Mixed-Valent Diiron Center of Methane Monooxygenase Hydroxylase from Methylococcus capsulatus (Bath) as Revealed by ENDOR Spectroscopy -- Chapter 3. An EPR Study of the Dinuclear Iron Site in the Soluble Methane Monooxygenase Reduced by One Electron at 77 K: the Effect of Component Interactions and the Binding of Small Molecules to the Dinuclear Ferric Center -- Chapter 4. An Investigation of the Reaction of Diferrous Methane Monooxygenase Hydroxylase with Dioxygen and Substrates by Rapid Freeze- Quench and Stopped-Flow Spectroscopy -- Chapter 5. Oxidation of Radical Clock Substrate Probes by the Soluble Methane Monooxygenase System -- Chapter 6. Tritiated Chiral Alkanes as Probes for the Mechanism of Hydroxylation by the Soluble Methane Monooxygenase.
by Ann M. Valentine.
Ph.D.
Harrison, John Samuel. "Production and characterisation of dioxygenase- and monooxygenase-catalysed oxidation products from a range of arenes and oxaarenes." Thesis, Queen's University Belfast, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301717.
Повний текст джерелаMohanty, Sujit Kumar. "A. Genetic characterization of the caffeine C-8 oxidation pathway in Pseudomonas Sp. CBB1 B. Validation of caffeine dehydrogenase as a suitable enzyme for a rapid caffeine diagnostic test." Diss., University of Iowa, 2013. https://ir.uiowa.edu/etd/4879.
Повний текст джерелаRay, Anirban. "Identification, Enumeration and Diversity of Nitrifying Bacteria in the Laurentian Great Lakes." Bowling Green State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1351276518.
Повний текст джерелаdela, Seña Carlo C. "Substrate specificity and reaction mechanism of vertebrate carotenoid cleavage oxygenases." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1396444100.
Повний текст джерелаMichel, Johannes Ehrenreich Josef. "Investigation and application of P450 monooxygenase for enantioselective oxidations /." Zürich : ETH, 2007. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=17076.
Повний текст джерелаBudde, Michael. "Biokatalyse mit Cytochrom P450 Monooxygenasen: zur selektiven Oxidation von Terpenen und Fettsäuren." [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-32363.
Повний текст джерелаVincent, Jean-Marc. "Réactivité chimique de complexes binucléaires de fer (III) à pont oxo : catalyse d'oxydation d'alcanes et agrégation d'espèces ferriques." Université Joseph Fourier (Grenoble ; 1971-2015), 1995. http://www.theses.fr/1995GRE10188.
Повний текст джерелаKranz-Finger, Sarah [Verfasser], Vlada B. [Gutachter] Urlacher, and Karl-Erich [Gutachter] Jaeger. "Oxidation pflanzlicher Triterpenoide mittels Cytochrom-P450-Monooxygenasen / Sarah Kranz-Finger ; Gutachter: Vlada B. Urlacher, Karl-Erich Jaeger." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2019. http://d-nb.info/1196870691/34.
Повний текст джерелаKranz-Finger, Sarah Katharina [Verfasser], Vlada B. [Gutachter] Urlacher, and Karl-Erich [Gutachter] Jaeger. "Oxidation pflanzlicher Triterpenoide mittels Cytochrom-P450-Monooxygenasen / Sarah Kranz-Finger ; Gutachter: Vlada B. Urlacher, Karl-Erich Jaeger." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2019. http://d-nb.info/1196870691/34.
Повний текст джерелаMcGhie, Emma Jane. "Studies on monooxygenases from the camphor degradation pathway in Pseudomonas putida NCIMB 10007 that are important in the catalysis of Baeyer-Villiger biotransformation reactions." Thesis, University of Exeter, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.390168.
Повний текст джерелаRabe, Volker. "Neue Nicht-Häm-Eisenkomplexe Synthese, spektroskopische Charakterisierung und Anwendung in der katalytischen aeroben C-H-Oxidation." Berlin mbv, Mensch-und-Buch-Verl, 2009. http://d-nb.info/1000289982/04.
Повний текст джерелаHamze, Khalil. "Baeyer-Villiger monooxygenases d'Acinetobacter : réactions biocatalysées et dédoublements cinétiques dynamiques." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM4311/document.
Повний текст джерелаEnzyme-mediated Baeyer-Villiger oxidation is nowadays largely recognized as an efficient method to obtain highly optically active lactones. An increasing number of Baeyer-Villiger Monooxygenases from various sources has been found to oxidize a large range of substrates with a good to excellent stereospecificity.Firstly, in order to enlarge the scope of these biotransformations, the genome of two strains of the Acinetobacter genus, A.baylyi ADP1 and A.baumannii AYE was explored. Six genes were expressed in E. coli and the substrate profile of each enzyme was studied using whole cell biotransformations. Four enzymes showed close substrate specificity with a preference for small cyclic ketones and for arylic substituents. Interestingly, one enzyme led to a Kinetic Parallel Regiodivergent Resolution of a bicycloheptenone and desymmetrisation of benzylic cyclobutanones in an enantiocomplementary manner when compared to the most of already known enzymes.The second part of this work describes the implementation of Dynamic Kinetic Resolution processes combining enzymatic BV oxidation and in situ racemization of α-substituted cyclohexanones to afford corresponding lactones in more than 50% yield. Cyclohexanone Monooxygenase (CHMO) from another Acinetobacter strain, A. calcoaceticus, was selected and the reactions were carried out with whole cells of producing CHMO E. coli strain. The racemization of α-substituted cyclohexanones, usually slowly racemized under basic conditions, was ensured by the use of containing phosphate salts or glycine buffer solutions. Several corresponding -caprolactones were isolated after methylation as enantiopure hydroxy methyl esters in 70-80% yield
Chabut, Barbara. "Complexes binucléaires à fer non-heminique : activité biomimétique et échange de ligands." Université Joseph Fourier (Grenoble ; 1971-2015), 1997. http://www.theses.fr/1997GRE10234.
Повний текст джерелаRimmel, Nina [Verfasser], Volker [Akademischer Betreuer] [Gutachter] Sieber та Wilfried [Gutachter] Schwab. "Selektive ω-Oxidation aliphatischer Substrate – Charakterisierung von Monooxygenasen und Etablierung einer Biotransformationsplattform / Nina Rimmel ; Gutachter: Volker Sieber, Wilfried Schwab ; Betreuer: Volker Sieber". München : Universitätsbibliothek der TU München, 2016. http://d-nb.info/1120013674/34.
Повний текст джерелаDuboc-Toia, Carole. "Réactivité chimique des complexes dinucléaires de fer (III) à pont oxo : applications en catalyse d'oxydation énantiosélective et en hydrolyse de phosphoesters." Université Joseph Fourier (Grenoble), 1998. http://www.theses.fr/1998GRE10092.
Повний текст джерелаBrondani, Patrícia Bulegon. "Investigação da seletividade de mono-oxigenases frente a substratos orgânicos de boro ou de selênio." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/46/46136/tde-03092012-142803/.
Повний текст джерелаIn this work we evaluated the selectivity (chemo or enantioselectivity) of four Baeyer-Villiger mono-oxigenases (BVMOS: PAMO, M446G PAMO, HAPMO and CHMO) in the presence of boron-containing or selenium-containing compounds. Initially, a series of boron-acetophenones were submitted to oxidation reactions mediated by BVMOs. The enzyme CHMO was chemoselective leading only to C-B bond transformation instead Baeyer-Villiger reaction. However, PAMO and PAMO M446G mediated both oxidations in 4-substituted substrates, and only the C-B transformation in 3-substituted substrates. The enzyme HAPMO leading to Baeyer- Villiger reaction and C-B transformation in all cases. When boron-containing alkenes were the substrates, only compounds with phenyl moiety in the structure were oxidized by BVMOs. It was observed only the C-B transformation and none of the epoxidation reaction. Chiral boron compounds were submitted to BVMOS mediated reactions in an attempt of enantioselective transformation. PAMO and M446G PAMO showed the best results leading, in most cases, to a satisfactory oxidation. However, only one compound was oxidized with great enantioselectivity (82-91% ee). Selenium-containing chiral compounds were also tested in reactions mediated by BVMOs. Again, PAMO showed the best results among BVMOs tested, but only when R2 e R1 = Ph the reaction occurred with great enantiosselectivity (97 % ee).
Bennati-Granier, Chloe. "Nouvelles enzymes fongiques pour l'amélioration de la dégradation de la biomasse lignocellulosique : étude des "Lytic Polysaccharide Monooxygenases" (LPMOs)." Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM4001.
Повний текст джерелаIn the current context, it becomes essential to make alternative to oil, such as the 2G bioethanol, available at large scale. However, the hydrolysis step by Trichoderma reesei enzymes remains the major bottleneck for an economically sustainable process. The present work is part of the Futurol project, and aims at identifying and characterizing new fungal enzymes to improve the hydrolysis of lignocellulosic biomass. From the proteomic data available for Podospora anserina and Fusarium verticillioides, a dozen of interesting enzymes were identified in their secretomes. This work focuses, mainly, on the AA9s « Lytic Polysaccharide Monooxygenases » (LPMOs) from P. anserina. Among all the LPMOs studied, PaLPMO9A, PaLPMO9E and PaLPMO9H that harbored a CBM1 were the most active on cellulose. Investigation of their regioselective mode of action revealed that PaLPMO9A and PaLPMO9H oxidatively cleaved at both C1 and C4 positions while PaLPMO9E released only C1-oxidized products. PaLPMO9H that was the most versatile in terms of substrate specificity as it also displayed activity on cello-oligosaccharides and β-(1,4)-linked hemicellulose polysaccharides (e.g., xyloglucan, glucomannan). The hydrolysis yield of the pretreated miscanthus was significantly improved up to 2 fold, when the PaLPMO9E, or PaLPMO9H were supplemented to the T. reesei cocktail. This work demonstrated the importance of these oxidative enzymes for lignocellulose deconstruction by fungi. These biocatalysts open new prospects to improve the enzymatic conversion of plant biomass for 2G bioethanol production
Ahirwar, Saurabh Kumar. "Exploring the monooxygenase activity and selectivity of two related Cytochrome P450 enzymes." Thesis, 2020. http://hdl.handle.net/2440/127956.
Повний текст джерелаThesis (MPhil) -- University of Adelaide, School of Physical Sciences, 2020
YU-CHIAOCHENG and 鄭宇喬. "Regio-Selective Oxidation of Alkyl Benzene Catalyzed by Recombinant Xylene Monooxygenase from Pseudomonas putida mt-2." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/76113619105529973307.
Повний текст джерела國立成功大學
化學系
104
Catalytic oxidation of toluene derivatives exhibits application for fine chemical supply in chemical industry. The corresponding transformation is essential to the remediation of environmental pollutants. XylMA system encoded by TOL plasmid for the protein expression of xylene monooxygenase (XylM) and its reductase (XylA) from Pseudomonas putida mt-2 was selected for an in-depth study. E. coli was employed as a host for their heterogeneously expression and purification. The catalytic conversions of alkyl benzenes were not only carried out by the whole cell catalysis mediated by the co-transformation of XylMA in E. coli system. To simplify the catalytic reaction without imposing expensive cofactor protein(s) or coenzymes, i.e. NAD(P)H, we herein also developed a facile electrochemical transformation system to conduct the direct electron transfer (DET) either directly to the intracytoplasmic membranes enriched with recombinant XylM or mediated by the iron-sulfur cofactor proteins such as XylA towards XylM protein. The X-ray absorption spectroscopy (XAS) studies of purified XylM was performed and displayed the core structure of active center in XylM protein is non-heme diiron center. Similar to recent discovery of the crystal structure of a mammalian stearoyl-CoA desaturase (Mouse SCD1), His-box (8-histidine motif) is essential for the coordination of the iron core complexes. Keywords: Xylene monooxygenase (XylM); XANES, EXAFS; Electrocatalysis
范錠明. "New inroads into the catalytic site and the mechanism of methane oxidation in the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) by novel mass spectrometry." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/61049342291856038878.
Повний текст джерелаBudde, Michael [Verfasser]. "Biokatalyse mit Cytochrom-P450-Monooxygenasen : zur selektiven Oxidation von Terpenen und Fettsäuren / vorgelegt von Michael Budde." 2007. http://d-nb.info/985833173/34.
Повний текст джерелаYang, Chung-Ling, and 楊宗霖. "Controlled oxidation of aliphatic C-H bonds in metallo-monooxygenases: Mechanistic insights derived from studies on deuterated and fluorinated hydrocarbons by Cytochrome P450 BM3." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/t2uwv5.
Повний текст джерела國立臺灣科技大學
應用科技研究所
103
The control oxidation of aliphatic C-H bonds in regio- and/or stereo-selective manner by metalloenzymes is of great interest to scientists. Cytochrome P450 BM3 from Bacillus megaterium oxidizes C12-C20 fatty acids at the ω-1, ω-2, or ω-3 position of the C?{H bond, respectively. We employ the molecular manipulation techniques to overexpress the recombinant BM3 strain and carry out its site-directed mutagenesis study for generation of a variety of strains towards variable selective oxidation with different substrates. In my study, strain 3mt, A74G F87V L188Q is exploited for the controlled oxidations of medium-chain length alkanes. Single mutation variant, BM3 mutant Ala328Phe (F328), was found with its capability of selective oxidation at the ω?{1 position of C4-C10 straight-chain alkanes. Interestingly, long-chain fatty acids (C12-C20) are no longer its substrates. It provides a great base to allow us engineering P450 BM3 protein from normal alkanes with medium-chain length such as n-octane gradually to smaller alkane such as n-butane for subsequent studies of controlled oxidation. The introduction of the second mutation, at Leu188Pro (P450 BM3-FP188) can improve the catalytic efficiency from butane to 2-butanol for 12.5%. In addtion, introducinge Ala74Glu (P450 BM3-FPE74) can shrink the substrate pocket down to half of the volume and significantly enhance the catalytic activity of butane to 2- butanol for nearly 40%. Typically, enzymes invoke host–guest chemistry to sequester the substrates within the protein pockets, exploiting sizes, shapes and specific interactions such as hydrogen-bonding, electrostatic forces and/or van der Waals interactions to control the substrate specificity, regio-specificity and stereo-selectivity. To further investigate this issue, we also develope a series of deuterated and fluorinated aliphatic substrates as probes to gain insights into the controlled C-H oxidations of hydrocarbons facilitated by these enzymes. The effects resulting from the introduction of deuterated butane (isotopomers) and fluorinated octane (Bioisostere) substituents on the mechanistic insights for C-H oxidation and the controls for regio-specificity and stereo-selectivity of the C-H bond activation are discussed.