Добірка наукової літератури з теми "Molecular sequestration"

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Статті в журналах з теми "Molecular sequestration"

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Berendt, A. R., D. J. P. Ferguson, J. Gardner, G. Turner, A. Rowe, C. McCormick, D. Roberts, et al. "Molecular mechanisms of sequestration in malaria." Parasitology 108, S1 (March 1994): S19—S28. http://dx.doi.org/10.1017/s0031182000075685.

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Cell surface molecules have received intense attention in recent years because of the central roles they play at the interface between the external environment and the cellular interior. Their functions include adhesion to other cells or extracellular matrices, protection against hostile physical, chemical and biological agents and the transport of metabolites into and out of the cell. In addition, cell surface molecules transduce signals across the cell membrane, relaying information inwards and presenting altered characteristics to the exterior as the environment changes.
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Hines, P. J. "Androgen-Driven Sequestration." Science Signaling 5, no. 254 (December 11, 2012): ec320-ec320. http://dx.doi.org/10.1126/scisignal.2003853.

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Lal, Rattan. "Carbon sequestration." Philosophical Transactions of the Royal Society B: Biological Sciences 363, no. 1492 (August 30, 2007): 815–30. http://dx.doi.org/10.1098/rstb.2007.2185.

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Developing technologies to reduce the rate of increase of atmospheric concentration of carbon dioxide (CO 2 ) from annual emissions of 8.6 Pg C yr –1 from energy, process industry, land-use conversion and soil cultivation is an important issue of the twenty-first century. Of the three options of reducing the global energy use, developing low or no-carbon fuel and sequestering emissions, this manuscript describes processes for carbon (CO 2 ) sequestration and discusses abiotic and biotic technologies. Carbon sequestration implies transfer of atmospheric CO 2 into other long-lived global pools including oceanic, pedologic, biotic and geological strata to reduce the net rate of increase in atmospheric CO 2 . Engineering techniques of CO 2 injection in deep ocean, geological strata, old coal mines and oil wells, and saline aquifers along with mineral carbonation of CO 2 constitute abiotic techniques. These techniques have a large potential of thousands of Pg, are expensive, have leakage risks and may be available for routine use by 2025 and beyond. In comparison, biotic techniques are natural and cost-effective processes, have numerous ancillary benefits, are immediately applicable but have finite sink capacity. Biotic and abiotic C sequestration options have specific nitches, are complementary, and have potential to mitigate the climate change risks.
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Berndt, J. D. "No Migration Without Sequestration." Science Signaling 6, no. 300 (November 5, 2013): ec268-ec268. http://dx.doi.org/10.1126/scisignal.2004878.

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Alvarez-Buylla, Aurora, Cheyenne Y. Payne, Charles Vidoudez, Sunia A. Trauger, and Lauren A. O’Connell. "Molecular physiology of pumiliotoxin sequestration in a poison frog." PLOS ONE 17, no. 3 (March 11, 2022): e0264540. http://dx.doi.org/10.1371/journal.pone.0264540.

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Poison frogs bioaccumulate alkaloids for chemical defense from their arthropod diet. Although many alkaloids are accumulated without modification, some poison frog species can metabolize pumiliotoxin (PTX 251D) into the more potent allopumiliotoxin (aPTX 267A). Despite extensive research characterizing the chemical arsenal of poison frogs, the physiological mechanisms involved in the sequestration and metabolism of individual alkaloids remain unclear. We first performed a feeding experiment with the Dyeing poison frog (Dendrobates tinctorius) to ask if this species can metabolize PTX 251D into aPTX 267A and what gene expression changes are associated with PTX 251D exposure in the intestines, liver, and skin. We found that D. tinctorius can metabolize PTX 251D into aPTX 267A, and that PTX 251D exposure changed the expression level of genes involved in immune system function and small molecule metabolism and transport. To better understand the functional significance of these changes in gene expression, we then conducted a series of high-throughput screens to determine the molecular targets of PTX 251D and identify potential proteins responsible for metabolism of PTX 251D into aPTX 267A. Although screens of PTX 251D binding human voltage-gated ion channels and G-protein coupled receptors were inconclusive, we identified human CYP2D6 as a rapid metabolizer of PTX 251D in a cytochrome P450 screen. Furthermore, a CYP2D6-like gene had increased expression in the intestines of animals fed PTX, suggesting this protein may be involved in PTX metabolism. These results show that individual alkaloids can modify gene expression across tissues, including genes involved in alkaloid metabolism. More broadly, this work suggests that specific alkaloid classes in wild diets may induce physiological changes for targeted accumulation and metabolism.
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Lioi, Sara B., Xiang Wang, Mohammad R. Islam, Emily J. Danoff, and Douglas S. English. "Catanionic surfactant vesicles for electrostatic molecular sequestration and separation." Physical Chemistry Chemical Physics 11, no. 41 (2009): 9315. http://dx.doi.org/10.1039/b908523h.

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Starkov, Anatoly A. "The molecular identity of the mitochondrial Ca2+ sequestration system." FEBS Journal 277, no. 18 (July 26, 2010): 3652–63. http://dx.doi.org/10.1111/j.1742-4658.2010.07756.x.

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Hu, Shichao, Wei Tang, Yan Zhao, Na Li, and Feng Liu. "Ultra-specific discrimination of single-nucleotide mutations using sequestration-assisted molecular beacons." Chemical Science 8, no. 2 (2017): 1021–26. http://dx.doi.org/10.1039/c6sc03048c.

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Coleman, Stewart M., Alan R. Prescott, and Judith E. Sleeman. "Transcriptionally correlated subcellular dynamics of MBNL1 during lens development and their implication for the molecular pathology of myotonic dystrophy type 1." Biochemical Journal 458, no. 2 (February 14, 2014): 267–80. http://dx.doi.org/10.1042/bj20130870.

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Sequestration of the alternative splicing factor MBNL1 in RNA foci is implicated in causing DM1. In eye lens cells from patients with DM1, MBNL1 sequestration to RNA foci appears not to be a major pathological event. MBNL1 does, however, show clear changes in subcellular distribution related to transcriptional activity and differentiation.
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Cho, Eun-Young, Dong-Im Cho, Jae H. Park, Hitoshi Kurose, Marc G. Caron, and Kyeong-Man Kim. "Roles of Protein Kinase C and Actin-Binding Protein 280 in the Regulation of Intracellular Trafficking of Dopamine D3 Receptor." Molecular Endocrinology 21, no. 9 (September 1, 2007): 2242–54. http://dx.doi.org/10.1210/me.2007-0202.

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Abstract D3 dopamine receptor (D3R) is expressed mainly in parts of the brain that control the emotional behaviors. It is believed that the improper regulation of D3R is involved in the etiology of schizophrenia. Desensitization of D3R is weakly associated with G protein-coupled receptor kinase (GRK)/β-arrestin-directed internalization. This suggests that there might be an alternative pathway that regulates D3R signaling. This report shows that D3R undergoes robust protein kinase C (PKC)-dependent sequestration that is accompanied by receptor phosphorylation and the desensitization of signaling. PKC-dependent D3R sequestration, which was enhanced by PKC-β or -δ, was dynamin dependent but independent of GRK, β-arrestin, or caveolin 1. Site-directed mutagenesis of all possible phosphorylation sites within the intracellular loops of D3R identified serine residues at positions 229 and 257 as the critical amino acids responsible for phorbol-12-myristate-13-acetate (PMA)-induced D3R phosphorylation, sequestration, and desensitization. In addition, the LxxY endocytosis motif, which is located between residues 252 and 255, was found to play accommodating roles for PMA-induced D3R sequestration. A continuous interaction with the actin-binding protein 280 (filamin A), which was previously known to interact with D3R, is required for PMA-induced D3R sequestration. In conclusion, the PKC-dependent but GRK-/β-arrestin-independent phosphorylation of D3R is the main pathway responsible for the sequestration and desensitization of D3R. Filamin A is essential for both the efficient signaling and sequestration of D3R.
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Дисертації з теми "Molecular sequestration"

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Johnson, Matthew David. "Physiological and molecular aspects of organelle sequestration in the ciliate myrionecta rubra." College Park, Md. : University of Maryland, 2005. http://hdl.handle.net/1903/3207.

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Thesis (Ph. D.) -- University of Maryland, College Park, 2005.
Thesis research directed by: Marine-Estuarine-Environmental Sciences. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.
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DEL, GIUDICE MARCO. "Molecular sequestration as a way to control variability and induce crosstalk: from theory to experiment." Doctoral thesis, Politecnico di Torino, 2018. http://hdl.handle.net/11583/2711198.

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The mechanism of molecular sequestration is involved in many biological processes, ranging from growth factors signalling to transcriptional and post-transcriptional regulation. This kind of dynamics involves two types of molecular species, targets and sequesters, that bind to form a complex. In the framework of mass-action law, key features of this kind of systems appear to be threshold-like profiles of the amounts of free molecules as a function of the parameters determining their possible maximum abundance. In biology, stochastic fluctuations, i.e. noise, play an undisputed role at the molecular level. Such noise can be usually divided into an intrinsic component, due to the probabilistic nature of biochemical processes, and an extrinsic one, related to the coupling with the variability of the environment in which reactions take place. Several studies highlighted the relevance of the variability induced by extrinsic fluctuations in shaping cell decision making and differentiation in molecular networks. Bimodal distributions of gene expression levels are a common feature of this kind of processes. Indeed, the two modes of the distribution often indicate the presence of two different physiological states of the system. In this thesis, we investigate the consequences of the introduction of a source of extrinsic noise onto a system governed by molecular sequestration, focussing on the appearance of bimodal distributions. To do that, we first study a minimal stochastic model of molecular sequestration and introduce extrinsic noise through a fluctuating parameter. In this framework, we analytically show how bimodal distributions can appear and characterize them as a result of noise filtering mediated by the threshold response. We then investigate the behavior of the correlations between targets of the same sequester and show how extrinsic fluctuations can induce a positive correlation that counterbalances the negative one due to competition. Given these results, we move to investigate the appearance of bimodal phenotypes in the context of microRNA (miRNA)-mediated gene regulation. MiRNAs are small noncoding RNA molecules that downregulate the expression of their target mRNAs. The interaction between miRNAs and targets is based on molecular sequestration and threshold-like responses are a known feature of this system. Recent studies suggested that miRNAs can be involved in the appearance of bimodal expression distributions of their target genes. To investigate this phenomenon, we characterize the system through an analytic and numerical approach and introduce extrinsic noise as a fluctuating miRNA transcription rate. We observe that bimodal distributions of target expression can appear for a wide range of parameters in presence of extrinsic noise. Furthermore, we show how the bimodal shape of the distribution can be tuned by the interplay between different target mRNAs competing for a common miRNA. In conclusion of this thesis we present some synthetic-biology experiments that are aimed at studying the role of extrinsic fluctuations in the appearance of bimodal distributions in the context of miRNA-mediated regulation.
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Abramov, Aleksandr. "Wettability of quartz surfaces under carbon dioxide geo-sequestration conditions. A theoretical study." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2019. https://ro.ecu.edu.au/theses/2232.

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The wettability of rocks under reservoir conditions is important to ensure and secure long term underground storage of carbon dioxide. The composition of those rocks vary significantly and are influenced by the fact that quartz is the second most abundant mineral in the earth's continental crust. Thus, the CO2 wettability of quartz dominates the overall CO2 trapping performance of storage and cap rocks. If depleted oil or gas reservoirs are used for storage of CO2 quartz surfaces of rocks in reservoirs which have been previously exposed to hydrocarbons might be covered with chemisorpt hydrocarbon molecules. The CO2 wettability of these chemically modified quartz is studied in this work with molecular dynamics. To model quartz surfaces with chemisorpt hydrocarbons both CLAYFF and DREIDING force fields are coupled at atomic site charge level using the density functional theory and the Bader charge analysis. Augmented with modified charges of the OC bond, CLAYFF and the DREIDING force fields are applied to solve the practical problem of calculating the contact angle of a water droplet on alkylated quartz surfaces in a CO2 environment. A systematic computational study of wettability of fully hydroxylated and alkylated (001) -quartz surface in carbon dioxide atmosphere with respect to surface concentration of pentyl groups is performed. Alkylated quartz surfaces have been shown to be extremely hydrophobic even when the surface density of hydroxyl groups is close to the highest naturally observed. The study also verifies that a comprehensive description of wettability of alkylated quartz surface requires three parameters: the theoretical contact angle, the apparent contact angle and the hidden contact angle. These contact angles are determined at the tip level of pentyl groups and the level of the quartz surface. The hidden contact angle is calculated as the angle of a water "skirt", which is formed between the level of the quartz surface and the tip level of pentyl groups. Additionally, the concept and the method of how to determine computational contact angles of a liquid droplet resting on a solid surface from individual snapshots of molecular dynamics simulations have been formulated, implemented and analysed in this work. Spherical coordinates to circumscribe a sphere around given configuration of water molecules form the basis of the method, which is thus natural and consistent with the droplet's geometric computational framework.
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Irani, Niloufer Gillan. "Cellular and molecular aspects of the transport and sequestration of anthocyanins in maize and Arabidopsis." Columbus, Ohio : Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1147381386.

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Sirikitputtisak, Tule. "Molecular simulation study of noble gas + n-decane binary mixtures at reservoir conditions." Thesis, University of Manchester, 2014. https://www.research.manchester.ac.uk/portal/en/theses/molecular-simulation-study-of-noble-gas--ndecane-binary-mixtures-are-reservoir-conditions(6ff06bf5-5bd4-4429-8c42-3ca96b4347c4).html.

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Carbon capture and sequestration are considered to be a temporary fix to the climate change global crisis. Following the noble gas tracers injection field experiment at Salt Creek in the state of Wyoming, USA, these tracers may be used to characterise the reservoir as a potential geological sequestration site for carbon dioxide. This study aims to investigate various thermodynamics properties of the five noble gases (Xe, Kr, Ar, Ne, and He) in n-decane at reservoir conditions (340 K – 460 K and 10 MPa – 200 MPa). The study utilises the SKS force field to describe n-decane and both Gibbs Ensemble Monte Carlo and molecular dynamics simulations were used to investigate the solubility, diffusivity, and vapour-liquid equilibrium of the five binary mixtures. The size of the noble gases was found to be important in these nonpolar mixtures where typical interactions are weak and short-ranged. The enthalpies of solvation were calculated and found to be directly correlated to the size of the solute where the energy required for the formation of a cavity to accommodate the solute is compensated by the nature of the intermolecular interaction between solvent and solute. The mixture of Ar + n-decane is of interest particularly because the sigma value for Ar is very similar to that of the CH3 group, resulting in the overall non-mononicity of several thermodynamics properties. Additionally, maxima in enthalpies of solvation were observed in Xe and Kr in n-decane solution at 200 MPa. While these maxima were observed in two different species at similar conditions, they are accommodated by unusually high uncertainties - further investigation is required before definitive conclusions can be drawn. The results from the vapour-liquid equilibrium study of the five noble gas + n-decane binary mixtures were in good agreement with the Peng-Robinson equation of state predictions. What is more, the diffusion coefficient ratios amongst the five noble gases in n-decane were investigated in light of Stoke-Einstein’s relation and Enskog’s hard-sphere relation. Three different radii of solute-solvent interaction were investigated and the best fit was observed when R =radius of solute + radius of gyration of n-decane. Additionally, the diffusion coefficients were utilised in the reservoir simulation to investigate the role of diffusion within the reservoir.
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Hassan, Faizule. "Adenovirus Mediated Delivery of Decoy Hyper Binding Sites for Sequestration of an Oncogenic Transcription Factor HMGA as a Potential Novel Cancer Therapy and Antibacterial Activity of Local Mushrooms." Miami University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=miami1511449587326648.

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Faust, Thomas Benjamin. "On the synthesis, measurement and applications of octanuclear heterometallic rings." Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/on-the-synthesis-measurement-and-applications-of-octanuclear-heterometallic-rings(a9697906-50e4-4d0a-9eda-bfd09b9e12f8).html.

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Inorganic macrocycles have stimulated interest in recent years for their magnetic properties, their associated host-guest chemistry and their aesthetically appealing structures. These characteristics have led to suggestions that they could be exploited for the purposes of ion recognition, catalysis, as single molecule magnets, MRI agents, antibacterial agents and as part of larger architectures in a molecular machine. This thesis explores the properties of a group of chromium(III) macrocycles, with functionality tailored towards different pursuits. Firstly the magnetic properties of a newly synthesised family of ring dimers are investigated. The nature of magnetic exchange within each ring leads to a net electronic spin which, it has been proposed, could represent a quantum binary digit within a quantum information processing system. By linking together pairs of rings, the degree of inter ring communication can be determined. Such interactions are important for the correlation of spin as initiation of quantum entanglement, a pre-requisite for quantum computing. The rings can also act as fluoro-metallocrowns, hosting the molecule which templated their formation. A range of rings with different guests are synthesised and their solid and solution state structures are explored. On templating about bulky dialkyl amines hybrid organic-inorganic rotaxanes are formed where the guest is fixed. In contrast when using small amines and alkali metals, exchange of guests is possible. The dynamics of all of these systems are investigated with proton NMR, quite remarkable for such highly paramagnetic complexes.
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Montavon, Thomas. "Biogénèse des siRNAs endogènes chez Arabidopsis thaliana : étude fonctionnelle de DRB7.2, une nouvelle protéine de fixation à l'ARN double brin et développement d'outils moléculaires pour la caractérisation du mode d'action de DCL4." Thesis, Strasbourg, 2017. http://www.theses.fr/2017STRAJ002/document.

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Les ARN double brin (ARNdb) sont les molécules clés initiatrices du RNA silencing, à partir desquelles les différentes classes de petits ARN (sRNAs), conférant la séquence spécificité de ce mécanisme, vont être produit. Chez la plante modèle Arabidopsis thaliana, le clivage des divers ARNdb en sRNAs est opéré par quatre enzymes de type RNase III, nommées DCL1 à DCL4, dont l’activité peut être assistée par des protéines fixant l’ARNdb (DRBs). Au cours de cette thèse, j’ai pu caractériser la fonction d’une nouvelle DRB, DRB7.2. Les résultats obtenus m’ont permis de démontrer que cette protéine régule la production d’une classe particulière de sRNAs endogènes, les endoIR-siRNAs, en séquestrant spécifiquement leurs précurseurs ARNdb, inhibant ainsi leur clivage par les différents DCLs. En parallèle, j’ai également développé des outils moléculaires afin d’étudier le mode d’action du DCL le plus polyvalent chez les plantes, DCL4. La caractérisation détaillée de ces outils a permis de révéler le rôle clé de déterminant structuraux distinct (protéiques ou nucléiques) impliqués dans la spécificité de reconnaissance et de clivage des divers substrats ARNdb par cette enzyme
RNA silencing is initiated by double-stranded RNA (dsRNA) molecules that will be processed into various classes of small RNAs (sRNAs), which confer the sequence-specificity of this mechanism. In the model plant Arabidopsis thaliana, dsRNA processing is mediated by four distinct RNaseIII-like enzymes, named DCL1 to DCL4, which can be assisted by dsRNA-binding proteins (DRBs). During my PhD, I was able to characterize in details the function of a new DRB protein, DRB7.2. Our results revealed that this protein regulates the accumulation of a specific class of endogenous sRNAs, the endoIR-siRNAs, by selectively sequestering their dsRNA precursors and inhibiting their cleavage by the DCLs. In parallel, I also developed molecular tools to study the mode of action of the most versatile DCL in plants, DCL4. Detailed characterization of these tools revealed key roles of distinct structural determinants (at the protein or RNA level), implicated in the specificity and cleavage efficiency of the various dsRNA susbtrates by DCL4
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Condron, Leo M. "Chemical nature and plant availability of phosphorus present in soils under long-term fertilised irrigated pastures in Canterbury, New Zealand." Lincoln College, University of Canterbury, 1986. http://hdl.handle.net/10182/1875.

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Soil P fractionation was used to examine changes in soil inorganic and organic P under grazed irrigated pasture in a long-term field trial at Winchmore in Mid-Canterbury. The soil P fractionation scheme used involved sequential extractions of soil with O.5M NaHCO₃ @ pH 8.5 (NaHCO₃ P), 0.1M NaOH (NaOH I P), 1M HCl (HCl P) and 0.1M NaOH (NaOH II P). The Winchmore trial comprised 5 treatments: control (no P since 1952), 376R (376 kg superphosphate ha⁻¹ yr⁻¹ 1952-1957, none since), 564R (564 kg superphosphate ha⁻¹ yr⁻¹ 1952-1957, none since) 188PA (188 kg superphosphate ha⁻¹ yr⁻¹ since 1952) and 376PA (376 kg superphosphate ha⁻¹ yr⁻¹ since 1952: Topsoil (0-7.5cm) samples taken from the different treatments in 1958, 1961, 1965, 1968, 1971, 1974 and 1977 were used in this study. Changes in soil P with time showed that significant increases in soil inorganic P occurred in the annually fertilised treatments (l88PA, 376PA). As expected, the overall increase in total soil inorganic P between 1958 and 1977 was greater in the 376PA treatment (159 µg P g⁻¹) than that in the 188PA treatment (37 µg P g⁻¹). However, the chemical forms of inorganic P which accumulated in the annually fertilised treatments changed with time. Between 1958 and 1971 most of the increases in soil inorganic P in these treatments occurred in the NaHCO₃ and NaOH I P fractions. On the other hand, increases in soil inorganic P in the annually fertilised treatments between 1971 and 1977 were found mainly in the HCl and NaOH II P fractions. These changes in soil P forms were attributed to the combined effects of lime addition in 1972 and increased amounts of sparingly soluble apatite P and iron-aluminium P in the single superphosphate applied during the 1970's. In the residual fertiliser treatments (376R, 564R) significant decreases in all of the soil inorganic P fractions (i.e. NaHCO₃ P, NaOH I P, HCl P, NaOH II p) occurred between 1958 and 1977 following the cessation of P fertiliser inputs in 1957. This was attributed to continued plant uptake of P accumulated in the soil from earlier P fertiliser additions. However, levels of inorganic P in the different soil P fractions in the residual fertiliser treatments did not decline to those in the control which indicated that some of the inorganic P accumulated in the soil from P fertiliser applied between 1952 and 1957 was present in very stable forms. In all treatments, significant increases in soil organic P occurred between 1958 and 1971. The overall increases in total soil organic P were greater in the annually fertilised treatments (70-86 µg P g⁻¹) than those in the residual fertiliser (55-64 µg P g⁻¹) and control (34 µg P g⁻¹) treatments which reflected the respective levels of pasture production in the different treatments. These increases in soil organic P were attributed to the biological conversion of native and fertiliser inorganic P to organic P in the soil via plant, animal and microbial residues. The results also showed that annual rates of soil organic P accumulation between 1958 and 1971 decreased with time which indicated that steady-state conditions with regard to net 'organic P accumulation were being reached. In the residual fertiliser treatments, soil organic P continued to increase between 1958 and 1971 while levels of soil inorganic P and pasture production declined. This indicated that organic P which accumulated in soil from P fertiliser additions was more stable and less available to plants than inorganic forms of soil P. Between 1971 and 1974 small (10-38 µg P g⁻¹) but significant decreases in total soil organic P occurred in all treatments. This was attributed to increased mineralisation of soil organic P as a result of lime (4 t ha⁻¹) applied to the trial in 1972 and also to the observed cessation of further net soil organic P accumulation after 1971. Liming also appeared to affect the chemical nature of soil organic P as shown by the large decreases in NaOH I organic P(78-88 µg P g⁻¹) and concomitant smaller increases in NaOH II organic P (53-65 µg P g⁻¹) which occurred in all treatments between 1971 and 1974. The chemical nature of soil organic P in the Winchmore long-term trial was also investigated using 31p nuclear magnetic resonance (NMR) spectroscopy and gel filtration chromatography. This involved quantitative extraction of organic P from the soil by sequential extraction with 0.1M NaOH, 0.2M aqueous acetylacetone (pH 8.3) and 0.5M NaOH following which the extracts were concentrated by ultrafiltration. Soils (0-7.5cm) taken from the control and 376PA annually fertilised treatments in 1958, 1971 and 1983 were used in this study. 31p NMR analysis showed that most (88-94%) of the organic P in the Winchmore soils was present as orthophosphate monoester P while the remainder was found as orthophosphate diester and pyrophosphate P. Orthophosphate monoester P also made up almost all of the soil organic P which accumulated in the 376PA treatment between 1958 and 1971. This indicated that soil organic P in the 376PA and control treatments was very stable. The gel filtration studies using Sephadex G-100 showed that most (61-83%) of the soil organic P in the control and 376PA treatments was present in the low molecular weight forms (<100,000 MW), although the proportion of soil organic P in high molecular weight forms (>100,000 MW) increased from 17-19% in 1958 to 38-39% in 1983. The latter was attributed to the microbial humification of organic P and indicated a shift toward more complex and possibly more stable forms of organic P in the soil with time. Assuming that the difference in soil organic P between the control and 376PA soils sampled in 1971 and 1983 represented the organic P derived from P fertiliser additions, results showed that this soil organic P was evenly distributed between the high and low molecular weight fractions. An exhaustive pot trial was used to examine the relative availability to plants of different forms of soil inorganic and organic P in long-term fertilised pasture soils. This involved growing 3 successive crops of perennial ryegrass (Lolium perenne) in 3 Lismore silt loam (Udic Ustochrept) soils which had received different amounts of P fertiliser for many years. Two of the soils were taken from the annually fertilised treatments in the Winchmore long term trial (188PA, 376PA) and the third (Fairton) was taken from a pasture which had been irrigated with meatworks effluent for over 80 years (65 kg P ha⁻¹ yr⁻¹). Each soil was subjected to 3 treatments, namely control (no nutrients added), N100 and N200. The latter treatments involved adding complete nutrient solutions with different quantities of N at rates of 100kg N ha⁻¹ (N100) and 200kg N ha⁻¹ (N200) on an area basis. The soil P fractionation scheme used was the same as that used in the Winchmore long-term trial study (i.e. NaHCO₃ P, NaOH I P, HCl P, NaOH II p). Results obtained showed that the availability to plants of different extracted inorganic P fractions, as measured by decreases in P fractions before and after 3 successive crops, followed the order: NaHCO₃ P > NaOH I P > HCl P = NaOH II P. Overall decreases in the NaHCO₃ and NaOH I inorganic P fractions were 34% and 16% respectively, while corresponding decreases in the HCl and NaOH II inorganic P fractions were small «10%) and not significant. However, a significant decrease in HCl P (16%) was observed in one soil (Fairton-N200 treatment) which was attributed to the significant decrease in soil pH (from 6.2 to 5.1) which occurred after successive cropping. Successive cropping had little or no effect on the levels of P in the different soil organic P fractions. This indicated that net soil organic P mineralisation did not contribute significantly to plant P uptake over the short-term. A short-term field experiment was also conducted to examine the effects of different soil management practices on the availability of different forms of P to plants in the long-term fertilised pasture soils. The trial was sited on selected plots of the existing annually fertilised treatments in the Winchmore long-term trial (188PA, 376PA) and comprised 5 treatments: control, 2 rates of lime (2 and 4 t ha⁻¹ ) , urea fertiliser (400kg N ha⁻¹ ) and mechanical cultivation. The above ground herbage in the uncultivated treatments was harvested on 11 occasions over a 2 year period and at each harvest topsoil (0-7.5 cm) samples were taken from all of the treatments for P analysis. The soil P fractionation scheme used in this particular trial involved sequential extractions with 0.5M NaHCO₃ @ pH 8.5 (NaHCO₃ P), 0.1M NaOH (NaOH P), ultrasonification with 0.1M NaOH (sonicate-NaOH p) and 1M HCl (HCl P). In addition, amounts of microbial P in the soils were determined. The results showed that liming resulted in small (10-21 µg P g⁻¹) though significant decreases in the NaOH soil organic P fraction in the 188PA and 376PA plots. Levels of soil microbial P were also found to be greater in the limed treatments compared with those in the controls. These results indicated that liming increased the microbial mineralisation of soil organic P in the Winchmore soils. However, pasture dry matter yields and P uptake were not significantly affected. Although urea significantly increased dry matter yields and P uptake, it did not appear to significantly affect amounts of P in the different soil P fractions. Mechanical cultivation and the subsequent fallow period (18 months) resulted in significant increases in amounts of P in the NaHCO₃ and NaOH inorganic P fractions. This was attributed to P released from the microbial decomposition of plant residues, although the absence of plants significantly reduced levels of microbial P in the cultivated soils. Practical implications of the results obtained in the present study were presented and discussed.
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Brousse, Valentine. "Déterminants biologiques, cellulaires et moléculaires de la dysfonction splénique chez l’enfant drépanocytaire Erythroid Adhesion Molecules in Sickle Cell Anaemia Infants: Insights Into Early Pathophysiology A novel non-invasive method to measure splenic filtration function in humans." Thesis, Sorbonne université, 2018. http://www.theses.fr/2018SORUS141.

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La drépanocytose est une maladie génétique du globule rouge (GR), due à une mutation ponctuelle du gène β de l’hémoglobine. L’objectif de ce travail était de caractériser lors de la commutation des hémoglobines chez l’enfant les modifications de propriétés cellulaires et moléculaires des GR afin de déterminer les mécanismes en jeu dans la perte de fonction splénique et la survenue de la séquestration splénique aigue (SSA). Nous avons travaillé sur les échantillons sanguins longitudinaux prélevés à 3-6 mois, 12, 18 et 24 mois de 57 enfants drépanocytaires. Nous avons pu mettre en évidence un profil d’expression de molécules d’adhérence spécifique à la surface des réticulocytes dès 3-6 mois. Ce profil d’adhérence spécifique est retrouvé au cours du temps sur les GR matures, se traduit fonctionnellement in vitro par une adhérence en flux accrue des GR sur des ligands d’intérêt (laminine, cellules endothéliales activées). L’exploration splénique séquentielle de la cohorte a permis de démontrer une perte de fonction dès 3-6 mois chez le nourrisson drépanocytaire asymptomatique s’aggravant à 18 mois et l’impact négatif de la SSA sur la fonction splénique. L’analyse des caractéristiques cellulaires a permis de démontrer le rôle du défaut de déformabilité et de l’adhérence des GR dans la genèse des lésions spléniques et de la SSA. Enfin ce travail a permis de montrer que la mesure précoce d’Hb et d’HbF permet d’identifier les nourrissons les plus à risque de complications sévères y compris de SSA
Sickle cell anemia (SCA) is a monogenic red blood cell (RBC) disorder due to a point mutation on the β-globin gene. In this project, we investigated RB cellular and molecular changes occurring during the Hb switch in order to decipher the mechanisms of splenic loss of function and acute splenic sequestration (ASS). Analysis was performed on longitudinal blood samples drawn at 3-6, 12 18 and 24 months in 57 SCD infants. First we were able to demonstrate a specific adhesion profile on reticulocytes of infants aged 3-6 months, thereafter evidenced on mature red cells as reticulocytes age and results in increased adhesion on laminin or activated endothelial cells in in vitro dynamic adhesion assays. Splenic function was measured sequentially in the cohort and showed that splenic function was impaired as early as 3-6 months and further impaired at 18 months. We also demonstrated that ASS further impacted negatively splenic function. We demonstrated that RBC loss of deformability and increased adhesion both play a major role in splenic loss of function and ASS. Our work also demonstrated the predictive value of HbF and Hb for severe SCA complications including ASS
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Книги з теми "Molecular sequestration"

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Farquhar-Smith, Paul. The key role of nerve growth factor in inflammatory pain processing. Edited by Paul Farquhar-Smith, Pierre Beaulieu, and Sian Jagger. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780198834359.003.0021.

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This paper was one of several that cemented the key role of nerve growth factor (NGF) in sensory neurons in inflammatory pain processing. It used a novel way of biologically ‘removing’ NGF using a sequestration molecule that could then investigate the effect of the absence of NGF in pain paradigms. These data contributed to the robust evidence base indicating the importance of NGF and, although there had been issues in developing usable anti-NGF moieties in human pain conditions, this study could be considered part of the journey that has led to the introduction of antagonists to NGF actions for the treatment of pain in bone.
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Частини книг з теми "Molecular sequestration"

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Yamanaka, Tomoyuki, and Nobuyuki Nukina. "Transcription Factor Sequestration by Polyglutamine Proteins." In Methods in Molecular Biology, 215–29. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-756-3_14.

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Kaur, Bupinderpal, Bhekam Pal Singh, and Yumnam Devashree. "Heavy Metal Sequestration in Plants." In Heavy Metals in Plants Physiological to Molecular Approach, 215–45. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003110576-11.

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Engedal, Nikolai, Morten Luhr, Paula Szalai, and Per O. Seglen. "Measurement of Bulk Autophagy by a Cargo Sequestration Assay." In Methods in Molecular Biology, 307–13. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8873-0_20.

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Braks, Joanna, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard. "Bioluminescence Imaging of P. berghei Schizont Sequestration in Rodents." In Methods in Molecular Biology, 353–68. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-026-7_25.

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Böhm, Alex, and Winfried Boos. "Transport-dependent gene regulation by sequestration of transcriptional regulators." In Molecular Mechanisms Controlling Transmembrane Transport, 47–66. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/b95774.

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Kurt, Fırat, and Ertugrul Filiz. "Isolation of quantitative trait loci/gene(s) conferring cadmium tolerance in sorghum." In Molecular breeding in wheat, maize and sorghum: strategies for improving abiotic stress tolerance and yield, 463–75. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789245431.0027.

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Abstract This chapter provides information on methods in identification of quantitative trait loci conferring cadmium tolerance in sorghum (Sorghum bicolor). Cadmium transport, tolerance, translocation, detoxification and sequestration in plants were also discussed.
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Yi, Li, Joseph M. Taft, Qing Li, Mark C. Gebhard, George Georgiou, and Brent L. Iverson. "Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast." In Methods in Molecular Biology, 81–93. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2748-7_5.

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Williamson, I., M. Lawson, E. B. Nelson, and L. Li. "Computational Modeling Study of MnO2 Octahedral Molecular Sieves for Carbon Dioxide-Capture Applications." In Materials and Processes for CO2 Capture, Conversion, and Sequestration, 344–55. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2018. http://dx.doi.org/10.1002/9781119231059.ch9.

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Aminoff, David. "Evidence for the Molecular Basis of Aging and Sequestration of Mammalian Erythrocytes." In Biomedical Advances in Aging, 63–73. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0513-2_6.

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Ohsumi, Y. "Physiological functions of vacuoles in yeast: Mechanism of sequestration of metabolites and proteins into vacuoles." In Molecular Biology and its Application to Medical Mycology, 39–45. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84625-0_6.

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Тези доповідей конференцій з теми "Molecular sequestration"

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Zhang, Yichi, Christian Cuba Samaniego, Katelyn Carleton, Yili Qian, Giulia Giordano, and Elisa Franco. "Building molecular band-pass filters via molecular sequestration." In 2022 IEEE 61st Conference on Decision and Control (CDC). IEEE, 2022. http://dx.doi.org/10.1109/cdc51059.2022.9993401.

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Samaniego, Christian Cuba, and Elisa Franco. "A molecular device for frequency doubling enabled by molecular sequestration." In 2019 18th European Control Conference (ECC). IEEE, 2019. http://dx.doi.org/10.23919/ecc.2019.8795656.

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Dey, Supravat, and Abhyudai Singh. "Stochastic analysis of feedback control by molecular sequestration." In 2019 American Control Conference (ACC). IEEE, 2019. http://dx.doi.org/10.23919/acc.2019.8814973.

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Samaniego, Christian Cuba, Jongmin Kim, and Elisa Franco. "Sequestration and delays enable the synthesis of a molecular derivative operator." In 2020 59th IEEE Conference on Decision and Control (CDC). IEEE, 2020. http://dx.doi.org/10.1109/cdc42340.2020.9304515.

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Korhonen, Anna, Maria Sundvall, Katri Vaparanta, Julius Anckar, Kalle Halkilahti, Zaidoun Salah, Rami Aqeilan, Jorma J. Palvimo, Lea Sistonen, and Klaus Elenius. "Abstract B182: Nuclear sequestration of ErbB4 receptor tyrosine kinase by PIAS3." In Abstracts: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics--Nov 12-16, 2011; San Francisco, CA. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1535-7163.targ-11-b182.

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Sandquist, Gary M., and Jay F. Kunze. "On-Line Sequestration of Fission Product Gases in Operating Nuclear Reactors." In 18th International Conference on Nuclear Engineering. ASMEDC, 2010. http://dx.doi.org/10.1115/icone18-29326.

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A research program is outlined to theoretically and experimentally establish the effectiveness and acceptability of on-line sequestering of fission product gases (FPGs) as they are generated in a fissile fuel pin using adsorbents and/or molecular sieves selectively distributed within the lower spring-plenum region of the LWR fuel. If successful this innovation can be implemented in existing and next generation LWRs and may: • reduce accident source term (releasable FPGs); • enhance reactor safety and acceptance; • extend fuel life suppressing clad swelling and fuel failure; • reduce thermal neutron poisons in high flux core regions; • improve operational and economical performance; • require only minor changes to fuel pin assembly and use; • use in LWRs with no license or tech-specifications impact.
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Bao, Kai, Mi Yan, Ligang Lu, Rebecca Allen, Amgad Salama, Kirk E. Jordan, and Shuyu Sun. "High-Performance Modeling of CO2 Sequestration by Coupling Reservoir Simulation and Molecular Dynamics." In SPE Reservoir Simulation Symposium. Society of Petroleum Engineers, 2013. http://dx.doi.org/10.2118/163621-ms.

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8

Pathak, Manas, Gorakh Pawar, Hai Huang, and Milind D. Deo. "Carbon Dioxide Sequestration and Hydrocarbons Recovery in the Gas Rich Shales: An Insight from the Molecular Dynamics Simulations." In Carbon Management Technology Conference. Carbon Management Technology Conference, 2015. http://dx.doi.org/10.7122/439481-ms.

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Sanlorenzo, Andrea, Marc Bustin, Amanda Bustin, and Gareth Chalmers. "CO2 Permeability in Shale Gas Reservoirs: Insights from the Montney Formation." In SPE Canadian Energy Technology Conference and Exhibition. SPE, 2023. http://dx.doi.org/10.2118/212793-ms.

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Abstract The Montney Formation, in north–eastern British Columbia and western Alberta, is a widely developed, low porosity and permeability shale gas and oil reservoir. Due to existing midstream infrastructure, it is an ideal candidate for CO2 sequestration which can potentially be coupled with CO2 enhanced hydrocarbon recovery (EHR). Extensive petrophysical analyses of representative Montney wells and cores validate that the characteristics of supercritical CO2 are more suitable for sequestration compared to either liquid or gas properties. The producing Montney reservoir has absolute permeabilities to helium in the order of 10−2 to 10−5 millidarcies and porosity ranging from 2.9 to 11.1%. At reservoir pressure and temperature conditions, sequestered carbon dioxide will be in the supercritical state. The measured apparent permeability of representative Montney cores matrix to supercritical CO2 is approximately 3.8×10−4 to 3.4×10−2 mD higher than either gas or liquid CO2 values (apparent supercritical CO2 permeabilities range between 4.0×10−4 and 1.4×10−2 mD). The difference between liquid and gas CO2 permeabilities ranges between 3.2×10−5 and 3.0×10−3 mD. Absolute permeabilities to helium were found to be higher than any of the three CO2 phases. The higher apparent permeability to supercritical CO2 compared to the gas or liquid phase is attributed to the higher molecular kinetic energy and the smaller impact of adsorption compared to gas CO2. Permeability data of gas CO2 show both volumetric and adsorption effects, resulting in a lower apparent permeability compared to both liquid and supercritical CO2. Helium data show the highest permeabilities since helium is a non-adsorbing gas and He molecular diameter is 74 pm smaller than the molecular diameter of CO2. The results of this study show that carbon dioxide in the supercritical state has favourable characteristics for the utilization and sequestration in depleted shale gas and oil plays compared to CO2 in either the liquid or gas phase. The relatively high density of the supercritical state – around 750 kg/m3 – will minimize leakage to adjacent formations. Upon reaching reservoirs’ minimum miscibility pressure, supercritical CO2 interfacial tension will approach zero and thus mixing with the residual liquid hydrocarbons will occur. The CO2 will cause the oil or condensate to swell, reducing the viscosity and thus improving the mobility and production rate of the remaining hydrocarbons in place.
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Zambon, G., M. Vigo, M. Scatigna, C. Di Giacomo, and P. Prandoni. "LOW MOLECULAR-WEIGHT HEPARIN CY216 IN THE TREATMENT OF ACUTE MASSIVE PULMONARY EMBOLISM." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643234.

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A 68 years old women presented to our Department with signs and symptoms suggestive of acute massive pulmonary embolism twenty days after knee fracture. She was in shock: heart rate, respiratory rate and blood pressure was 140/m, 36/m and 70 mmHg respectively. Two months before she had a severe episode of cerebral haemorrhage. Clinical suspicion was confirmed by a pulmonary digital subtraction angiography (DSA) and computerized tomography (CT), which showed a large filling defect in the right main pulmonary artery and a high degree of fascular sequestration of the lung (Miller index = 18). After rejecting the use of thrombolytic drugs, it was decided to treat the patient with low molecular-weight heparin CY216, due to the possible weaker bleeding potential with respect to the unfractionated heparin, and a dose of 17,500 U anti-Xa Choay was administered subcutaneously two times daily. A significant clinical improvement (decrease of heart and respiratory rate and increase of blood pressure) was observed within the first 48 hours of treatment. Pulmonary DSA and CT scan, repeated after 7 days of treatment, showed an important reduction of the above-mentioned defects (Miller index = 7). Not any bleeding complication developed and the patient was discharged within 15 days from the Hospital on warfarin therapy.
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Звіти організацій з теми "Molecular sequestration"

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Criscenti, Louise Jacqueline, and Jacquelyn Bracco. Molecular modeling in support of CO2 sequestration and enhanced oil recovery. Office of Scientific and Technical Information (OSTI), January 2011. http://dx.doi.org/10.2172/1010420.

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Goddard, William. Recovery Act: Molecular Simulation of Dissolved Inorganic Carbons for Underground Brine CO2 Sequestration. Office of Scientific and Technical Information (OSTI), November 2012. http://dx.doi.org/10.2172/1082428.

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Jeffrey D. Evanseck, Jeffry D. Madura, and Jonathan P. Mathews. Use of molecular modeling to determine the interaction and competition of gases within coal for carbon dioxide sequestration. Office of Scientific and Technical Information (OSTI), April 2006. http://dx.doi.org/10.2172/882469.

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Evanseck, Jeffrey, Jeffry Madura, and Jonathan Mathews. Use of Molecular Modeling to Determine the Interaction and Competition of Gases Within Coal for Carbon Dioxide Sequestration. Office of Scientific and Technical Information (OSTI), September 2006. http://dx.doi.org/10.2172/915749.

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Jeffrey D. Evanseck and Jeffry D. Madura. Use of Molecular Modeling to Determine the Interaction and Competition of Gases within Coal for Carbon Dioxide Sequestration. Office of Scientific and Technical Information (OSTI), February 2003. http://dx.doi.org/10.2172/922134.

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Jeffrey D. Evanseck, Jeffry D. Madura, and Jonathan P. Mathews. USE OF MOLECULAR MODELING TO DETERMINE THE INTERACTION AND COMPETITION OF GASES WITHIN COAL FOR CARBON DIOXIDE SEQUESTRATION. Office of Scientific and Technical Information (OSTI), May 2004. http://dx.doi.org/10.2172/826305.

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7

Jeffrey D. Evanseck, Jeffry D. Madura, and Jonathan P. Mathews. USE OF MOLECULAR MODELING TO DETERMINE THE INTERACTION AND COMPETITION OF GASES WITHIN COAL FOR CARBON DIOXIDE SEQUESTRATION. Office of Scientific and Technical Information (OSTI), May 2005. http://dx.doi.org/10.2172/841533.

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Galili, Gad, and Alan Bennett. Role of Molecular Chaperone in Wheat Storage Protein Assembly. United States Department of Agriculture, April 1995. http://dx.doi.org/10.32747/1995.7604926.bard.

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Following sequestration into the ER, wheat gliadins assemble into complexes that initiate the formation of protein bodies. In the present work we have characterized the DNA sequence and regulation of expression of a plant BiP and also studied its interaction with wheat storage proteins as well as its role in the maturation of these storage proteins. In the Israeli lab, immunoprecipitation studies were made using anti BiP and anti storage proteins sera, both in wheat and in transgenic tobacco plants expressing a wheat gliadin storage proteins. In both cases, we could show that BiP interacts with the gliadin storage proteins. In addition, we could show that BiP also played an important role in the initial assembly of the gliadins. In the American lab, the complexity, structure and properties of tomato BiP was characterized at the molecular and biochemical levels. In addition, tomato BiP was also overexpressed in bacteria and the overexpressed protein was found to be active. The cooperative findings of the Israeli and American labs clearly improves our understanding of the structure and expression of a plant BiP as well as its role in the maturation of storage proteins in plants seeds. In addition, it will serve as a foundation for future studies of the mechanisms of BiP function in in vitro studies using purified storage proteins and purified recombinant active BiP.
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