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Статті в журналах з теми "MiRNA-mRNA interactions"
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Guo, Li, Yang Zhao, Sheng Yang, Hui Zhang, and Feng Chen. "Integrative Analysis of miRNA-mRNA and miRNA-miRNA Interactions." BioMed Research International 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/907420.
Повний текст джерелаАнотація:
MicroRNAs (miRNAs) are small, noncoding regulatory molecules. They are involved in many essential biological processes and act by suppressing gene expression. The present work reports an integrative analysis of miRNA-mRNA and miRNA-miRNA interactions and their regulatory patterns using high-throughput miRNA and mRNA datasets. Aberrantly expressed miRNA and mRNA profiles were obtained based on fold change analysis, and qRT-PCR was used for further validation of deregulated miRNAs. miRNAs and target mRNAs were found to show various expression patterns. miRNA-miRNA interactions and clustered/homologous miRNAs were also found to contribute to the flexible and selective regulatory network. Interacting miRNAs (e.g., miRNA-103a and miR-103b) showed more pronounced differences in expression, which suggests the potential “restricted interaction” in the miRNA world. miRNAs from the same gene clusters (e.g., miR-23b gene cluster) or gene families (e.g., miR-10 gene family) always showed the same types of deregulation patterns, although they sometimes differed in expression levels. These clustered and homologous miRNAs may have close functional relationships, which may indicate collaborative interactions between miRNAs. The integrative analysis of miRNA-mRNA based on biological characteristics of miRNA will further enrich miRNA study.
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Muniategui, Ander, Rubén Nogales-Cadenas, Miguél Vázquez, Xabier L. Aranguren, Xabier Agirre, Aernout Luttun, Felipe Prosper, Alberto Pascual-Montano, and Angel Rubio. "Quantification of miRNA-mRNA Interactions." PLoS ONE 7, no. 2 (February 14, 2012): e30766. http://dx.doi.org/10.1371/journal.pone.0030766.
Повний текст джерела
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Naderi, Elnaz, Mehdi Mostafaei, Akram Pourshams, and Ashraf Mohamadkhani. "Network of microRNAs-mRNAs Interactions in Pancreatic Cancer." BioMed Research International 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/534821.
Повний текст джерелаАнотація:
Background.MicroRNAs are small RNA molecules that regulate the expression of certain genes through interaction with mRNA targets and are mainly involved in human cancer. This study was conducted to make the network of miRNAs-mRNAs interactions in pancreatic cancer as the fourth leading cause of cancer death.Methods.56 miRNAs that were exclusively expressed and 1176 genes that were downregulated or silenced in pancreas cancer were extracted from beforehand investigations. MiRNA–mRNA interactions data analysis and related networks were explored using MAGIA tool and Cytoscape 3 software. Functional annotations of candidate genes in pancreatic cancer were identified by DAVID annotation tool.Results.This network is made of 217 nodes for mRNA, 15 nodes for miRNA, and 241 edges that show 241 regulations between 15 miRNAs and 217 target genes. The miR-24 was the most significantly powerful miRNA that regulated series of important genes. ACVR2B, GFRA1, and MTHFR were significant target genes were that downregulated.Conclusion.Although the collected previous data seems to be a treasure trove, there was no study simultaneous to analysis of miRNAs and mRNAs interaction. Network of miRNA-mRNA interactions will help to corroborate experimental remarks and could be used to refine miRNA target predictions for developing new therapeutic approaches.
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Subat, Sophia, Kentaro Inamura, Hironori Ninomiya, Hiroko Nagano, Sakae Okumura, and Yuichi Ishikawa. "Unique MicroRNA and mRNA Interactions in EGFR-Mutated Lung Adenocarcinoma." Journal of Clinical Medicine 7, no. 11 (November 6, 2018): 419. http://dx.doi.org/10.3390/jcm7110419.
Повний текст джерелаАнотація:
The EGFR gene was one of the first molecules to be selected for targeted gene therapy. EGFR-mutated lung adenocarcinoma, which is responsive to EGFR inhibitors, is characterized by a distinct oncogenic pathway in which unique microRNA (miRNA)–mRNA interactions have been observed. However, little information is available about the miRNA–mRNA regulatory network involved. Both miRNA and mRNA expression profiles were investigated using microarrays in 155 surgically resected specimens of lung adenocarcinoma with a known EGFR mutation status (52 mutated and 103 wild-type cases). An integrative analysis of the data was performed to identify the unique miRNA–mRNA regulatory network in EGFR-mutated lung adenocarcinoma. Expression profiling of miRNAs and mRNAs yielded characteristic miRNA/mRNA signatures (19 miRNAs/431 mRNAs) in EGFR-mutated lung adenocarcinoma. Five of the 19 miRNAs were previously listed as EGFR-mutation-specific miRNAs (i.e., miR-532-3p, miR-500a-3p, miR-224-5p, miR-502-3p, and miR-532-5p). An integrative analysis of miRNA and mRNA expression revealed a refined list of putative miRNA–mRNA interactions, of which 63 were potentially involved in EGFR-mutated tumors. Network structural analysis provided a comprehensive view of the complex miRNA–mRNA interactions in EGFR-mutated lung adenocarcinoma, including DUSP4 and MUC4 axes. Overall, this observational study provides insight into the unique miRNA–mRNA regulatory network present in EGFR-mutated tumors. Our findings, if validated, would inform future research examining the interplay of miRNAs and mRNAs in EGFR-mutated lung adenocarcinoma.
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Mukushkina, D. D., S. Labeit, and A. T. Ivashchenko. "CHARACTERISTICS OF miRNA INTERACTION WITH mRNA OF ISCHEMIC HEART DISEASE CANDIDATE GENES." REPORTS 335, no. 1 (February 12, 2021): 74–82. http://dx.doi.org/10.32014/2021.2518-1483.11.
Повний текст джерелаАнотація:
Ischemic heart disease (IHD) is the most serious cardiovascular disease and one of the leading causes of death worldwide. An important role in the pathophysiology of IHD play such processes as the processes of inflammation and immune response, metabolism of homocysteine and folate, development processes of endothelial dysfunction and oxidative stress and homeostasis system. Accordingly, the identified genes that are directly involved in these processes. In addition, miRNA (mRNA-inhibiting RNA) may affect the expression of these candidate genes. Using bioinformatics methods, the most efficient associations of miRNA and target genes were established. This research presents the characteristics of miRNA interactions with mRNA of candidate IHD genes. Candidate genes were identified that had a free energy of interaction with miRNA equal to -120 kJ / mole and higher in the following interactions: in 5’UTR - ALDH2 and ID02142.3p-miR; CELSR2 and ID00457.3p-miR; DDAH2 and ID01272.3p-miR; DNMT1 and ID02052.5p-miR; DOCK7 and ID00061.3p-miR; EGFR and ID02457.3p-miR; FOLH1 and ID01428.3p-miR; IL6R and miR-6089; NOS3 and ID02363.5p-miR; NPC1 and ID00551.3p-miR; PPP1R17 and ID01693.5p-miR; PRKCH and ID00520.5p-miR; SERPINE1 and ID01098.3p-miR; in CDS - ABCG8 and ID03064.3p-miR; ADORA2A and ID02697.3p-miR; APOA1 and ID00457.3p-miR; CDKN2B and ID02899.3p-miR; IL6R and ID01806.3p-miR; TIMP2 and ID00098.5p-miR; TNF and ID02050.3p-miR; TRIB1 and ID03208.5p-miR; VWF and ID01238.5p-miR. Associations were also revealed in the 3'UTR region with an interaction free energy of -115 kJ/mole and higher: AGTR2 and ID01213.5p-miR; APLNR and ID00616.5p-miR; CXCL12 and ID00483.3p-miR; FADS2 and miR-1224-3p; FCGR2A and miR-1273g-3p; GCKR and ID02928.3p-miR; IL6R and ID00913.5p-miR; KCNJ11 and ID03288.5p-miR; PPP1R3B and ID00913.5p-miR; TFPI and miR-1273g-3p; TIMP2 and ID01941.5p-miR. The results obtained could be used as molecular genetic markers of IHD for the diagnosis of this disease.
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Bencun, Maja, Thiago Britto-Borges, Jessica Eschenbach, and Christoph Dieterich. "New Tricks with Old Dogs: Computational Identification and Experimental Validation of New miRNA–mRNA Regulation in hiPSC-CMs." Biomedicines 10, no. 2 (February 6, 2022): 391. http://dx.doi.org/10.3390/biomedicines10020391.
Повний текст джерелаАнотація:
Cardiovascular disease is still the leading cause of morbidity and mortality worldwide. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have become a valuable widespread in vitro model to study cardiac disease. Herein, we employ the hiPSC-CM model to identify novel miRNA–mRNA interaction partners during cardiac differentiation and β-adrenergic stress. Whole transcriptome and small RNA sequencing data were combined to identify novel miRNA–mRNA interactions. Briefly, mRNA and miRNA expression profiles were integrated with miRNA target predictions to identify significant statistical dependencies between a miRNA and its candidate target set. We show by experimental validation that our approach discriminates true from false miRNA target predictions. Thereby, we identified several differentially expressed miRNAs and focused on the two top candidates: miR-99a-5p in the context of cardiac differentiation and miR-212-3p in the context of β-adrenergic stress. We validated some target mRNA candidates by 3′UTR luciferase assays as well as in transfection experiments in the hiPSC-CM model system. Our data show that iPSC-derived cardiomyocytes and computational modeling can be used to uncover new valid miRNA–mRNA interactions beyond current knowledge.
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Alshalalfa, Mohammed. "MicroRNA Response Elements-Mediated miRNA-miRNA Interactions in Prostate Cancer." Advances in Bioinformatics 2012 (November 4, 2012): 1–10. http://dx.doi.org/10.1155/2012/839837.
Повний текст джерелаАнотація:
The cell is a highly organized system of interacting molecules including proteins, mRNAs, and miRNAs. Analyzing the cell from a systems perspective by integrating different types of data helps revealing the complexity of diseases. Although there is emerging evidence that microRNAs have a functional role in cancer, the role of microRNAs in mediating cancer progression and metastasis remains not fully explored. As the amount of available miRNA and mRNA gene expression data grows, more systematic methods combining gene expression and biological networks become necessary to explore miRNA function. In this work I integrated functional miRNA-target interactions with mRNA and miRNA expression to infer mRNA-mediated miRNA-miRNA interactions. The inferred network represents miRNA modulation through common targets. The network is used to characterize the functional role of microRNA response element (MRE) to mediate interactions between miRNAs targeting the MRE. Results revealed that miRNA-1 is a key player in regulating prostate cancer progression. 11 miRNAs were identified as diagnostic and prognostic biomarkers that act as tumor suppressor miRNAs. This work demonstrates the utility of a network analysis as opposed to differential expression to find important miRNAs that regulate prostate cancer.
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Stebel, Sophie, Janina Breuer, and Oliver Rossbach. "Studying miRNA–mRNA Interactions: An Optimized CLIP-Protocol for Endogenous Ago2-Protein." Methods and Protocols 5, no. 6 (November 30, 2022): 96. http://dx.doi.org/10.3390/mps5060096.
Повний текст джерелаАнотація:
Transcriptome-wide analysis of RNA-binding partners is commonly achieved using UV crosslinking and immunoprecipitation (CLIP). Individual-nucleotide-resolution CLIP (iCLIP)enables identification of the specific position of the protein–RNA interaction. In addition to RNA-binding proteins (RBPs), microRNA (miRNA)–mRNA interactions also play a crucial role in the regulation of gene expression. Argonaute-2 (Ago2) mediates miRNA binding to a multitude of mRNA target sites, enabling the identification of miRNA–mRNA interactions by employing modified Ago2-CLIP protocols. Here, we describe an Ago2-specific CLIP protocol optimized for the use of small quantities of cell material, targeting endogenous Ago2 while avoiding possible methodological biases such as metabolic labeling or Ago2 overexpression and applying the latest advances in CLIP library preparation, the iCLIP2 protocol. In particular, we focus on the optimization of lysis conditions and improved radioactive labeling of the 5′ end of the miRNA.
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Afonso-Grunz, Fabian, and Sören Müller. "Principles of miRNA–mRNA interactions: beyond sequence complementarity." Cellular and Molecular Life Sciences 72, no. 16 (June 3, 2015): 3127–41. http://dx.doi.org/10.1007/s00018-015-1922-2.
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Wang, Zixing, Wenlong Xu, Haifeng Zhu, and Yin Liu. "A Bayesian Framework to Improve MicroRNA Target Prediction by Incorporating External Information." Cancer Informatics 13s7 (January 2014): CIN.S16348. http://dx.doi.org/10.4137/cin.s16348.
Повний текст джерелаАнотація:
MicroRNAs (miRNAs) are small regulatory RNAs that play key gene-regulatory roles in diverse biological processes, particularly in cancer development. Therefore, inferring miRNA targets is an essential step to fully understanding the functional properties of miRNA actions in regulating tumorigenesis. Bayesian linear regression modeling has been proposed for identifying the interactions between miRNAs and mRNAs on the basis of the integrated sequence information and matched miRNA and mRNA expression data; however, this approach does not use the full spectrum of available features of putative miRNA targets. In this study, we integrated four important sequence and structural features of miRNA targeting with paired miRNA and mRNA expression data to improve miRNA-target prediction in a Bayesian framework. We have applied this approach to a gene-expression study of liver cancer patients and examined the posterior probability of each miRNA-mRNA interaction being functional in the development of liver cancer. Our method achieved better performance, in terms of the number of true targets identified, than did other methods.
Дисертації з теми "MiRNA-mRNA interactions"
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Fu, Xiaonan. "Functional study of miRNA-mRNA interactions in malaria mosquito An. gambiae." Diss., Virginia Tech, 2018. http://hdl.handle.net/10919/96216.
Повний текст джерелаАнотація:
Female adults of many mosquito species possess distinct physiological features adapting to blood feeding for successful reproduction. The disease pathogens that are transmitted by mosquitoes have evolved to take advantages of the indispensable blood feedings to complete their transmission cycles and to survive attacks from the mosquito's innate immune system. Normal egg development and mosquito immunity are tightly controlled by tissue- and stage-specific gene expression and coordinated by many signal molecules in the mosquito. Understanding gene regulation affecting mosquito reproduction and malaria parasites infection is of paramount importance for developing novel malaria control strategies. A growing body of evidence indicates that microRNAs (miRNAs) are involved in egg maturation and immune reactions against invading pathogens in mosquitoes. However, the molecular mechanisms by which specific miRNAs selectively modulate reproduction and the survival of pathogens are largely unknown.
The miRNA-induced gene-silencing pathway in mosquitoes was mostly extrapolated from the studies of flies. To explore the dynamics of miRNAs in reproduction, I used small RNAs sequencing to monitor miRNAs expression and their association with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) in the malaria mosquito Anopheles gambiae (An. gambiae) during the 72-h period immediately after blood feeding. I found the abundance and Ago loading of most of the mature miRNAs were relatively stable after blood ingestion. However, miRNAs of the miR-309/286/2944 cluster were considerably upregulated after blood feeding. I confirmed that miR-309 is essential for normal egg development by depletion of endogenous miR-309 with a specific antagomir. In addition, my results showed that the Ago association of some miRNAs was not proportional to their cellular abundance implying additional regulation at miRNA integration.
To investigate the functional roles of miRNAs and define context-dependent miRNA-mRNA interactions during the reproductive process, I have applied an innovative experimental approach to study miRNA-mRNA interactome. CLEAR (covalent ligation of endogenous Argonaute-bound RNAs)-CLIP can generate miRNA-mRNA chimeras from UV-irradiation stabilized Ago-miRNA-mRNA complex. My results have defined tens of thousands of miRNA-mRNA interactions in mosquitoes, including novel targets for mosquito-specific miRNAs. Verification of the predicted interactions using mRNA-seq, ribosome-profiling, and luciferase reporter assay revealed a reliable miRNA-mRNA interaction network. Based on the detected interactions, I refined the paring rules for mosquito miRNAs and illustrated the dynamic pairing between different regions of miRNAs with their targets in vivo. The miRNA-mRNA interactions were compared using this approach at multiple time points before and after blood feeding. Importantly, this study showed that the interactions were dynamic and enriched in genes that are involved in metabolisms, supporting the proposed functions of miRNAs in coordinating the gene regulation in mosquito reproduction.
Plasmodium falciparum (P. falciparum) is a major human malaria parasite. To understand the functions of miRNAs in the mosquito resistance to Plasmodium infection, we analyzed the miRNA-mRNA interactions after female mosquitoes taking a P. falciparum-infected blood meal or an uninfected blood meal. Comparison of the interactions revealed enhanced miRNA-mRNA interactions after P. falciparum infection involving a group of immunity-related genes. In summary, this study has provided a systematic view and significantly advanced our understanding of the miRNA functions in mosquito reproduction and P. falciparum infection.PHD
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Shahab, Shubin. "Microrna and messenger rna interactions in ovarian cancer." Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/44753.
Повний текст джерелаАнотація:
Regulation of gene expression is a complex process in mammalian cells with many levels of control. In recent years non-coding RNAs in the form of microRNAs (miRNA) have surfaced as important regulators of protein coding genes, with biologically important roles in development, differentiation and cell growth. In this dissertation the complex interactions between miRNAs and mRNAs in ovarian cancer are investigated using a combination of computational and experimental techniques. In vitro studies and current models predict that increases in levels of miRNA should result in corresponding decreases in the levels of targeted mRNAs due to miRNA induced degradation. Profiling the global miRNA and mRNA expression patterns in epithelial ovarian cancer cells from patients and surface epithelial cells from normal ovaries reveal only ~11% of predicted targets of miRNAs are inversely correlated in vivo. In an effort to dissect the mechanisms behind these unexpected observations single miRNA transfection experiments are carried out followed by gene expression profiling. Analysis of genes altered following these transfections reveal majority of the altered genes are not direct targets of the miRNAs. Network analysis however suggests that miRNAs may target "hub genes" to cause altered expression in downstream transcripts. Pathway enrichment analysis of altered genes demonstrates miRNAs may regulate specific pathways rather than causing random off-target effects. Finally investigation of miRNA regulation reveals miRNAs may also affect the levels of other miRNAs, which may indirectly affect more genes downstream. Together these results provide a detailed view of the mechanisms employed by miRNAs to regulate the expression of hundreds of genes in ovarian cancer cells.
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Frampton, Adam. "The complex network of miRNA and mRNA target interactions in pancreatic cancer." Thesis, Imperial College London, 2014. http://hdl.handle.net/10044/1/24951.
Повний текст джерелаАнотація:
Pancreatic cancer (pancreatic ductal adenocarcinoma, PDAC) is one of the most lethal tumour types world-wide. The majority of patients present late with locally advanced or metastatic disease. Therefore, despite advances in operative techniques, perioperative management and oncological treatments, the overall 5-year survival remains <5%. Determining tumoural factors that contribute towards its aggressive nature may help in identifying novel molecular biomarkers and/or therapeutic targets. MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate target gene expression and are able to act as tumour suppressors or oncogenes. MiRNAs have been extensively profiled and implicated in the initiation and progression of PDAC. Furthermore, there is a possibility of translating miRNAs into clinically useful biomarkers. Here, I developed upon these initial observations and demonstrate that miRNAs can be used to differentiate low risk pancreatic benign cystic tumours (BCTs) from PDAC. We confirmed that these miRNAs regulate the expression of known PDAC oncogenes, and that miR-16, miR-126 and let-7d target BCL2, CRK and KRAS respectively. Next, in order to investigate the main contributors to tumourigenesis, an integrated molecular analysis (miRNA-mRNA) was performed in PDAC. By using a combination of network-based bioinformatics, miR-21, miR-23a and miR-27a were prioritised as important in PDAC progression. We demonstrated that the use of a combination of miRNA inhibitors (against miR-21, miR-23a and miR-27a) in a murine subcutaneous PDAC xenograft model was able to reduce tumour growth, better than oncomiR-21 inhibition alone. BTG2 and NEDD4L were found to be direct targets of the miRNA combination and were established as new candidate tumour suppressors in PDAC. The clinical relevance of this 3 miRNA signature was demonstrated, as high expressors of the combination have poor overall survival after surgical resection, independent of other clinicopathologic factors. Together, these studies identify specific miRNAs as important regulators of PDAC tumourigenesis and their possible use as biomarkers.
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Hernandes, Natalia Helena. "Identificação e validação das interações miRNA-mRNA na metamorfose de Apis mellifera." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/17/17135/tde-04012017-115203/.
Повний текст джерелаАнотація:
A metamorfose em insetos é um dos mais complexos e belos eventos biológicos conhecidos, dirigido por sucessivas alterações morfo-fisiológicas. Este intricado processo é coordenado por componentes moleculares como ecdisteroides (20E) e hormônio juvenil (HJ), fatores de transcrição e microRNAs (miRNAs). Os miRNAs regulam a expressão de genes-alvo, que por sua vez orquestram alterações fisiológicas e anatômicas necessárias para o completo desenvolvimento do organismo. Apesar do enorme esforço, os circuitos genéticos e endócrinos que regulam a metamorfose em insetos sociais, como a abelha Apis mellifera, estão longe de serem completamente esclarecidos. Os miRNAs são importantes componentes da maquinaria celular e parecem ser ubíquos no controle de processos biológicos. Desvendar novas interações miRNA-mRNAs alvo envolvidas com a metamorfose e a regulação das cascatas de 20E e HJ lançará uma luz sobre esse complexo evento. Em nosso estudo nós investigamos os papéis de miR-34, miR-281, miR-252a e miR-252b, conhecidos como reguladores da metamorfose em insetos, no modelo A. mellifera. Todos estes miRNAs revelaram alto grau de conservação filogenética, bem como responderam ao tratamento com 20E, sofrendo flutuações na abundância de transcritos. Usando as informações disponíveis e nossos bancos de dados, nós identificamos interações envolvendo estes miRNAs e genes participantes nas cascatas de HJ e 20E: ultraspiracle (Usp), fushi tarazu-transcription factor 1 (ftz-f1), ecdysone receptor (EcR), calponin (chd64), insulin receptor 2 (inr2), e Krüppel homolog 1 (Krh1). A predição das interações miRNA-mRNAs alvo revelou que os receptores de ecdisteroides EcR e Usp, bem como o fator de transcrição ftz-f1 são alvos importantes dos miRNAs estudados, apresentando sítios para os quatros miRNAs investigados. Observamos também que os seis genes codificadores de proteína são putativamente alvejados por miR-34. Por meio do ensaio da luciferase, pudemos validar as interações entre miR-34 e os alvos Kr-h1, chd64 e inr2; miR-252a e os alvos ftz-f1 e EcR; miR-252b e os alvos chd64 e ftz-f1; miR-281 e os alvos ftz-f1, EcR e Usp. A investigação dos perfis de expressão dos miRNAs ao longo do desenvolvimento larval (L3-PP3) e pupal (Pw), contrastados com os perfis de seus respectivos alvos, apontou muitos casos de relações positivas miRNA-mRNA. Estes resultados complementaram os resultados de validação, e expuseram a regulação exercida pelo miRNA sobre seus alvos. Juntos, os nossos resultados apontam para novas interações miRNA-mRNAs, envolvidas com a metamorfose em A. mellifera. As regulações por nós propostas e validadas bem como suas caracterizações e relações com os hormônios reguladores da metamorfose, são inéditas e acrescentam muito ao conhecimento sobre a regulação da metamorfose em A. mellifera. Nesse contexto, nossa pesquisa definitivamente contribui para uma melhor compreensão dos eventos moleculares envolvidos com a metamorfose de abelhas.Insect metamorphosis is one of the most complex and beautiful of known biological events; it consists of successive morphological and physiological alterations. This intricate process is coordinated by various molecular components, including ecdysteroids (20E), juvenile hormone (JH), transcription factors and microRNAs (miRNAs). The miRNAs regulate gene expression, which in turn orchestrates physiological and anatomical changes necessary for successful insect ontogeny. Despite enormous efforts, the endocrine and genetic circuits that regulate metamorphosis in social insects, such as honey bees (Apis mellifera), are far from being completely elucidated. The miRNAs are a substantial component of this molecular machinery and seem to be ubiquitously involved in the control of biological processes. Disclosing new miRNA-target interactions involved in metamorphosis and in the regulation of 20E and JH cascades can shed light on these poorly understood events. In this study, we provide new pieces to this puzzle. We investigated the roles of miR-34, miR-281, miR-252a and miR-252b, known to be important regulators of insect metamorphosis, in the A. mellifera model. All of these miRNAs revealed a high degree of phylogenetic conservation and responded to treatment with 20E, which altered transcript abundance. Using available information and our databases, we identified interactions involving these miRNAs and the component genes of JH and 20E pathways: ultraspiracle (Usp), fushi tarazu-transcription factor 1 (ftz-f1), ecdysone receptor (EcR), calponin (chd64), insulin receptor 2 (inr2), and Krüppel homolog 1 (Kr-h1). Prediction of miRNA-target interactions revealed that the ecdysteroid receptors EcR and Usp and the transcription factor ftz-f1 are highly targeted by miRNAs involved in metamorphosis; they presented binding sites for all four miRNAs. We also observed that all six-protein coding genes are putatively targeted by miR-34. Using the luciferase assay, we were able to validate the interactions of miR-34 with the targets Krh1, chd64 and inr2; miR-252a with the targets ftz-f1 and EcR; miR-252b with the targets chd64 and ftz-f1; and miR-281 with the targets ftz-f1, EcR and Usp. Investigation of miRNA expression profiles during larval (L3-PP3) and pupal (Pw) development, as a function of the profiles of their respective targets, demonstrated many cases of positive miRNA-mRNA relationships. These results complemented the validation results, showing how the miRNAs regulate their targets. In conclusion, we identified various previously unknown miRNA-mRNA interactions involved in the metamorphosis of A. mellifera. The regulatory pathways proposed and validated by us, as well as their characterizations and relationships with metamorphosis regulator hormones, are unique and add to the understanding of the regulation of metamorphosis in A. mellifera. In this context, our research contributes to a better understanding of the molecular events involved in honey bee metamorphosis.
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Homberg, Nicolas. "New models and algorithms for the identification of sncRNA-(snc)RNA interactions intra and across-species/kingdom." Electronic Thesis or Diss., Lyon 1, 2023. http://www.theses.fr/2023LYO10090.
Повний текст джерелаАнотація:
Les microARNs (miARNs) sont de petit ARNs non codant présents dans tous les eucaryotes qui régulent, positivement ou négativement, l'expression des ARN messagers (ARNms). Les miARNs ont un potentiel important pour de futurs traitements du cancer et d'autres maladies. Les interactions miARN-ARNm dépendent d'une variété de mécanismes complexes, tels que la complémentarité des séquences, l'accessibilité et la conservation. Cette thèse se concentre sur deux de ces mécanismes, à savoir l'accessibilité et la conservation intra-espèce du site d'interaction, en utilisant des données expérimentales de Cross-linking, Ligation And Sequencing of Hybrids (CLASH). Bien que l'accessibilité des sites d'interaction sur les ARNms soit généralement observée, cela n'est pas le cas pour toutes les interactions. La conservation intra-espèce est un mécanisme peu considéré que nous avons étudiée au travers la recherche de motifs conservés dans les ARNms. Bien que les résultats obtenus soient bruités, il est possible de retrouver via ces motifs certains sites d'interaction sur les ARNmsMicroRNAs (miRNAs) are non-coding RNAs present in eukaryotes that regulate the expression of messenger RNAs (mRNAs) up or down. These miRNAs have significant potential in future treatment of cancer and other diseases. The miRNA-mRNA interactions are intricate and involve various mechanisms, such as sequence complementarity, accessibility, and conservation. This thesis focuses on two such mechanisms, namely accessibility and intra-species conservation of the site of interaction, using experimental data from Cross-linking, Ligation And Sequencing of Hybrids (CLASH). Although the accessibility of interaction sites on mRNAs is generally observed, it is not consistent for all interactions. Intra-species conservation is a rare feature, which we explore by inferring conserved motifs from mRNA interaction sites. Although the results are noisy, in some specific cases, we manage to retrieve some mRNA interaction sites from the inferred motifs
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Lo, Tzu-Chun, and 羅子鈞. "A Statistical Framework for Identifying miRNA-mRNA Interactions in Association Studies." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/2bm4p6.
Повний текст джерелаАнотація:
碩士國立中正大學
資訊工程研究所
103
MicroRNAs (miRNAs) are noncoding small RNAs which suppress target mRNA expres- sion by cleavage. Thanks to the development of small RNA sequencing (small RNA-Seq) and RNA sequencing (RNA-Seq), we can gain insight into the landscape and expres- sion abundance of miRNAs and mRNAs in the genome. In reality, the miRNA-cleaved transcripts remain in the cell before degradation. These cleaved transcripts may be still captured and sequenced, leading to aberrant expression around the cleavage site. In this thesis, we design and implement a statistical framework for identifying aberrant expression caused by miRNA cleavage from RNA-seq. Our method is applied on a cold- stress study of two rice strains. We identied 23 miRNA-mRNA interactions with such aberrant expression, which are highly correlated to tolerance of cold stress.
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Correia, Sónia Cristina Heleno. "Molecular analysis of microRNA-target gene interactions in the pine seed." Master's thesis, 2017. http://hdl.handle.net/10362/21586.
Повний текст джерелаАнотація:
Pinus pinaster is a conifer species with high ecologic and economic value which covers a vast area of the Mediterranean region. However, the area occupied by P. pinaster has been decreasing mainly due to environmental causes. Somatic embryogenesis may contribute to circumvent this problem as it allows the large-scale propagation of plants adapted to several stresses. However, this technique is poorly understood in P. pinaster and the study of gene expression regulators like miRNAs may contribute to its improvement.
MiRNAs regulate gene expression at post-transcriptional level by mRNA cleavage or translation repression. In this work, a set of four miRNA-mRNA target cleavage interactions were studied. First, mRNAs targets were predicted for a list of miRNAs using bioinformatics tools to analyze available sequencing data, and seven miRNA-mRNA pairs were selected. Second, the expression of four of the mRNAs were analyzed by RT-PCR in two embryo developmental stages, T4B (pre-cotyledonary) and T7 (mature). The four transcripts included ARF 10, 16 ou 17 (regulated by miR160), F-box (regulated by miR482a), LEA (regulated by miRM09664) and PPR (regulated by miRM06658). All transcripts show a higher expression in T4B than in T7 stage. Several parameters were studied to ensure that high-confidence pairs were selected, namely conservation of miRNA and respective target, complementarity between miRNA and respective target, relation between miRNA expression and target expression. Only miRNA160-ARF target fulfilled all the conditions.
It was the first time that potential miRNA targets were identified in P. pinaster. This work contributed to uncover the miRNA landscape in conifers, pointing to potential functions in embryo development. However, more experiments are needed to further validate the interaction between the miRNA-mRNA targets studied.
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Rana, Mitali. "miRNA-mRNA interaction map in breast cancer." Thesis, 2013. http://ethesis.nitrkl.ac.in/5304/1/411LS2062.pdf.
Повний текст джерелаАнотація:
MicroRNAs are a class of small endogenous RNA molecules that is involved in the posttranscriptional inhibition of gene expression. They directly interact with target gene transcripts and influence cellular physiology. MicroRNAs have been reported to be involved in breast cancer tumorigenesis and metastasis thus playing a vital role in cancer progression. Our study aims at identification of novel miRNA-mRNA target pairs that are hypothesized to play a role in breast cancer through a miRNA- mRNA interaction map analysis of microarray data and experimental validation of selected set of mRNAs. The target interaction map analysis revealed three novel target pairs, hsa-miR-27a–MARCKS, hsa-miR-27a–SIK1 and hsa-miR-21–BTG2 which can be potential therapeutic targets in breast cancer. Therefore, with the better understanding of the regulation of miRNAs, the gene networks and cellular pathways regulated by miRNAs, it will be of immense significance to further comprehend breast cancer pathogenesis and target interaction as a therapeutic for breast cancer.
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Czarnocki-Cieciura, Mariusz. "Analiza architektury kompleksu CCR4-NOT i mechanizmu jego działania w szlaku mikroRNA." Doctoral thesis, 2015.
Знайти повний текст джерелаАнотація:
Końce eukariotycznych cząsteczek mRNA są na terenie cytoplazmy zabezpieczone przed degradacją przez strukturę 5'-kapu oraz ogon poli(A) dołączany do ich 3'-końca. Białka PABP wiążące ogon poli(A) oddziałują ponadto z czynnikami inicjacji translacji eIF4E/eIF4G wiążącymi strukturę 5'-kapu. Prowadzi to do cyrkularyzacji transkryptu, która stabilizuje taki kompleks rybonukleoproteinowy i wzmacnia jego translację. Pierwszym etapem degradacji cząsteczki mRNA jest więc zablokowanie oddziaływania ze sobą jej końców poprzez skrócenie ogona poli(A) (deadenylacja przez kompleksy CCR4-NOT oraz PAN2-PAN3) lub usunięcie struktury 5'-kapu przez kompleks DCP2-DCP1. Kompleks CCR4-NOT jest główną eukariotyczną deadenylazą i odpowiada za pierwsze etapy degradacji większości mRNA na terenie cytoplazmy. Jest on silnie zachowany ewolucyjnie i składa się z przynajmniej dwóch niezależnych modułów funkcjonalnych oddziałujących z jego największą podjednostką, białkiem CNOT1. Dwie podjednostki katalityczne (CNOT6/CNO6L oraz CNOT7/CNOT8) wiążą się razem z białkiem CNOT9 do N-końcowej części białka CNOT1, podczas gdy pozostałe białka NOT (CNOT2 oraz CNOT3) oddziałują z jego C-końcową częścią.Kompleks CCR4-NOT jest również powiązany ze szlakiem posttranskrypcyjnego wyciszania genów przez mikroRNA. miRNA są krótkimi, 22 nukleotydowymi cząsteczkami RNA, które po wbudowaniu w kompleksy RISC (RNA-induced silencing complex) wyciszają ekspresję wybranych genów. Wiążą się one do komplementarnych sekwencji znajdujących się w obszarze 3'-UTR określonych transkryptów i powodują ich degradację oraz represję translacji. Kluczową rolę odgrywają przy tym białka z rodziny GW182. Ich N-końcowa domena wiąże się do kompleksów RISC, podczas gdy ich C-końcowa domena SD/CED (silencing domain/C-terminal effector domain) odpowiada za wyciszanie transkryptów. Oddziałuje ona bezpośrednio z białkami odpowiedzialnymi za degradację mRNA, w tym z kompleksem CCR4-NOT, ale molekularny mechanizm tego oddziaływania nie jest znany. By zbadać oddziaływania pomiędzy podjednostkami kompleksu CCR4-NOT w układzie in vitro oraz mechanizm jego rekrutacji do transkryptów wyciszanych na szlaku miRNA, wybrane podjednostki ludzkiego kompleksu CCR4-NOT oraz fragmenty białek GW182 oczyszczono wykorzystując ekspresję heterologiczną w bakteriach E. coli. Oddziaływania pomiędzy rekombinowanymi białkami zbadano wykorzystując analizę wielokątowego rozpraszania światła sprzężoną z sączeniem molekularnym (SEC-MALS). Dzięki niej wykazano, że za wiązanie podjednostki CNOT7 odpowiada centralnie położona domena MIF4G białka CNOT1, natomiast podjednostka CNOT9 oddziałuje z sąsiadującą z nią domeną CN9BD. Analizy przeprowadzone na drożdżowych ortologach podjednostek CNOT2 i CNOT3 wykazały ponadto, że białka te oddziałują ze sobą poprzez C-końcowe domeny NOT-box. Systematyczna analiza oddziaływań pomiędzy podjednostkami i modułami kompleksu CCR4-NOT a domeną SD/CED ludzkich białek GW182 umożliwiła ustalenie dwóch obszarów odpowiedzialnych za jego rekrutację do transkryptów związanych przez kompleksy RISC. Znajdują się one na powierzchni podjednostki CNOT9 oraz w obrębie modułu NOT złożonego z C-końców białek CNOT1, CNOT2 oraz CNOT3. Wiązanie kompleksu CCR4-NOT przez białka GW182 jest ponadto zależne od łańcuchów bocznych tryptofanów znajdujących się w nieustrukturyzowanych obszarach domeny SD/CED. Powyższe wyniki pozwalają lepiej zrozumieć architekturę kompleksu CCR4-NOT oraz wyjaśniają molekularny mechanizm jego rekrutacji do wyciszanych transkryptów przez białka z rodziny GW182.Eukaryotic mRNA are protected from degradation from both ends by the cap structure and poly(A) tail at their 5' and 3' ends, respectively. Moreover, the PABP proteins (poly(A) binding proteins) associated with poly(A) tail interact with the translation factors eIF4E/eIF4G that protect the 5' cap structure. This mRNA circularization stabilizes such mRNP (mRNA-protein complexes) and results in the enhancement of translation. Therefore, the first step in mRNA degradation is destabilization of the interaction between 5'- and 3'-ends by reducing the length of poly(A) tail (deadenylation by the CCR4-NOT and PAN2-PAN3 protein complexes) and/or removal of the 5'-cap structure by the DCP2-DCP1 decapping complex.The CCR4-NOT complex is the major eukaryotic deadenylase and is involved in cytoplasmic degradation of most mRNA molecules. This evolutionarily conserved multiprotein assembly consists of at least two distinct functional and structural modules that are connected by the large scaffold protein CNOT1. The two catalytic subunits (CNOT6/CNOT6L and CNOT7/CNOT8) together with the CNOT9 protein interact with the N-terminal part of CNOT1 protein, while the CNOT2 and CNOT3 proteins associate with the C-terminal part of CNOT1. Recently it was shown that CCR4-NOT complex is involved in microRNA-mediated mRNA repression. Central to this pathway are small, 22 nt long RNA called miRNA. They are complementary to short sequences present in 3'-UTR (untranslated region) of many transcripts. After incorporation into RNA-induced silencing complexes (RISC) miRNA guide them to those mRNA. This generally leads to translational repression and subsequent degradation of targeted transcripts. Major players in this process are GW182 proteins. They are recruited to miRNA targets through their N-terminal domains, while their C-terminal part, called silencing domain (SD) or C-terminal effector domain (CED), is directly responsible for silencing. The SD/CED domain may directly recruit deadenylase complexes and presumably other degradation factors by interacting with CNOT1 protein, but the exact mechanism of GW182 action remains elusive. To get insights into architecture of the CCR4-NOT complex and to identify its subunits that are directly involved in the interaction with GW182 proteins a series of truncated versions of CNOT1 protein and other subunits of the human CCR4-NOT complex were purified from E. coli expression cells. Recombinant proteins were tested for interaction with each other and with SD/CED domain of human GW182 proteins by size-exclusion chromatography coupled with multi-angle light scattering (SEC-MALS). These experiments showed that the CNOT7 catalytic subunit interacts with the central domain of CNOT1 protein called MIF4G while the CNOT9 subunit is bound to the adjacent CN9BD domain. Moreover, experiments conducted with yeast orthologues of CNOT2 and CNOT3 subunits showed that they interact with each other through their C-terminal NOT-box domains. SEC-MALS analysis of CCR4-NOT subunits mixed with SD/CED domain showed that the GW182 proteins recruit the major deadenylase complex by interacting with the CNOT9 subunit. This interaction is mediated by hydrophobic Trp residues scattered throughout the unstructured parts of SD/CED sequence and is further stabilised by additional binding surface located on the NOT-module of the CCR4-NOT complex. Collectively, these results provide new insights into the architecture of the CCR4-NOT complex and explain mechanism of its recruitment by GW182 proteins.
Частини книг з теми "MiRNA-mRNA interactions"
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Andrés-León, Eduardo, Gonzalo Gómez-López, and David G. Pisano. "Prediction of miRNA–mRNA Interactions Using miRGate." In Methods in Molecular Biology, 225–37. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6866-4_15.
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Bagnacani, Andrea, Markus Wolfien, and Olaf Wolkenhauer. "Tools for Understanding miRNA–mRNA Interactions for Reproducible RNA Analysis." In Computational Biology of Non-Coding RNA, 199–214. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8982-9_8.
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Luo, Dan, Shu-Lin Wang, and Jianwen Fang. "Combining Gene Expression and Interactions Data with miRNA Family Information for Identifying miRNA-mRNA Regulatory Modules." In Intelligent Computing Theories and Application, 311–22. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-63312-1_28.
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Kléma, Jiří, Jan Zahálka, Michael Anděl, and Zdeněk Krejčík. "Interaction-Based Aggregation of mRNA and miRNA Expression Profiles to Differentiate Myelodysplastic Syndrome." In Biomedical Engineering Systems and Technologies, 165–80. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-26129-4_11.
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Giurgiu, Miruna, Robert Kaltenbach, Franziska Ahrend, Summer Weeks, Holly Clifton, Martin Bouldo, Vitaly Voloshin, Jiling Zhong, Siegfried Harden, and Alexander Kofman. "Multiple genetic polymorphisms within microRNA targets and homologous microRNA-binding sites: two more factors influencing microRNA-mediated regulation of gene expression." In Advances in Genetic Polymorphisms [Working Title]. IntechOpen, 2023. http://dx.doi.org/10.5772/intechopen.1002250.
Повний текст джерелаАнотація:
miRNA-mRNA interaction depends on multiple factors such as 3’UTR isoforms, the cell and tissue-specific expression levels of RNA-binding proteins, the sequence context around the mRNA target site, and other mechanisms. Genetic polymorphisms within miRNAs and their target sites appear to be among the most important ones because they influence the mode and outcome of miRNA-mRNA interaction universally and irreversibly. SNP disruption of miRNAs and their binding sites, as well as conformational changes preventing the access of the miRNA to its target site, are adopted as the most credible mechanistic explanations of SNP-mediated effects. The occurrence of multiple SNPs within the same miRNA-binding site implies their combinatorial mode of action. The presence of the repetitive (homologous) binding sites for the same miRNA on its mRNA target may both enhance the miRNA targeting and provide for the backup target site instead of the one disrupted by SNP, thus rescuing the miRNA functionality. While being underexplored, the multiple genetic polymorphisms within the miRNA-binding sites, as well as homologous miRNA-binding sites, may be considered as additional factors influencing miRNA-mediated regulation of gene expression.
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Shahid, Imran, and Qaiser Jabeen. "Appling Drug Discovery in HCV-therapeutics: A snapshot from the past and glimpse into the future." In Hepatitis C Virus-Host Interactions and Therapeutics: Current Insights and Future Perspectives, 290–342. BENTHAM SCIENCE PUBLISHERS, 2023. http://dx.doi.org/10.2174/9789815123432123010013.
Повний текст джерелаАнотація:
The ongoing COVID-19 pandemic with its devastating impacts in terms of huge disease burden and patient management on the world’s leading healthcare systems and jolting the world’s biggest economies, has leveraged the lesson that to prevent the transmission and elimination of a viral pandemic, endemic, or epidemic in future, a prophylactic or protective vaccine would be indispensable. In this scenario, DAAs regimens alone would not be sufficient to eliminate the HCV epidemic by 2030 or beyond and there would always be the demand for a prophylactic or protective vaccine to prevent the transmission of this epidemic again from vulnerable populations. The anti-mRNA-based treatment strategies (e.g., anti-HCV protein-specific oligonucleotides, RNA interference (RNAi), and micro RNA (miRNA)), and some potential anti-hepatitis C vaccine models have been widely and extensively studied as an alternative or adjuvant therapeutic approaches for hepatitis C in the recent past and some of those models are still in the pipeline. The approval of the first RNAi therapy against a hereditary protein deposition disorder has urged investigators to refocus this approach against hepatitis C because it represents the most thoroughly studied treatment strategy against hepatitis C in the last two decades. Furthermore, some emerging approaches like host targeting agents (HTA), nanoparticles-containing immunogens, and nanomedicine-based therapeutic agents are also in their full investigative form. In this book chapter, we will discuss and highlight emerging hepatitis C treatment approaches that could be the game-changer to vanquishing HCV by 2030 while used as an adjuvant or compensatory regimen with DAAs.
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Thi Ngoc Nguyen, Thanh, Thu Huynh Ngoc Nguyen, Luan Huu Huynh, Hoang Ngo Phan, and Hue Thi Nguyen. "Predicting SNPs in Mature MicroRNAs Dysregulated in Breast Cancer." In Recent Advances in Non-Coding RNAs [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.105514.
Повний текст джерелаАнотація:
Breast cancer (BC) is the leading type of cancer among women. Findings have revolutionized current knowledge of microRNA (miRNA) in breast tumorigenesis. The seed region of miRNA regulates the process of gene expression negatively. The presence of SNPs in the seed regions of miRNA dramatically alters the mature miRNA function. Additionally, SNPs in the out-seed region of miRNAs have a significant impact on miRNA targeting. This study focuses on the in silico analysis procedure of mature miRNA SNPs and their impact on BC risk. The database annotated SNPs on mature miRNAs was used. Also, target gene alterations, miRNAs function in BC, and the interaction of miRNAs with targets were predicted. A list of 101 SNPs in 100 miRNAs with functional targets in BC was indicated. Under the SNPs allele variation, 10 miRNAs changed function, 6 miRNAs lost targets, 15 miRNAs gained targets, 48 onco-miRNAs remained unchanged, and 21 tumor suppressor miRNAs remained unchanged. At last, a list of 89 SNPs, which alter miRNA function and miRNA-mRNA interaction, were shown to be potentially associated with BC risk. This research theoretically generated a list of possible causative SNPs in the mature miRNA gene that might be used in future BC management studies.
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Majeed Shah, Ishteyaq, Mashooq Ahmad Dar, Kaiser Ahmad Bhat, Tashook Ahmad Dar, Fayaz Ahmad, and Syed Mudasir Ahmad. "Long Non-Coding RNAs: Biogenesis, Mechanism of Action and Role in Different Biological and Pathological Processes." In Recent Advances in Non-Coding RNAs [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.104861.
Повний текст джерелаАнотація:
RNA or ribonucleic acid constitutes of nucleotides, which are ribose sugars coupled to nitrogenous bases and phosphate groups. Nitrogenous bases include adenine, guanine, cytosine and uracil. Messenger RNA, ribosomal RNA and Transfer RNA are three main types of RNA that are involved in protein synthesis. Apart from its primary role in synthesis of protein, RNA comes in variety of forms like snRNA, miRNA, siRNA, antisense RNA, LncRNA etc., that are involved in DNA replication, post-transcriptional modification, and gene regulation etc. LncRNAs regulate gene expression by various ways including at, transcriptional, post-transcriptional, translational, post-translational and epigenetic levels by interacting principally with mRNA, DNA, protein, and miRNA. Among other biological functions, they are involved in chromatin remodelling, transcriptional interference, transcriptional activation, mRNA translation and RNA processing. In this chapter we shall be discussing the origin of lncRNAs, their biogenesis, their mechanism of action and their role in many biological and pathological processes like epigenetics, genome imprinting, several cancers and autoimmune diseases.
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Prado-Garcia, Heriberto, Ana E. González-Santiago, Susana Romero-Garcia, Alejandra Garcia-Hernandez, Victor Ruiz, Arnoldo Aquino-Galvez, Alma Cebreros-Verdin, and Angeles Carlos-Reyes. "lncRNA-miRNA-mRNA Interaction Networks Regulation in Hematological Malignancies." In Reference Module in Biomedical Sciences. Elsevier, 2023. http://dx.doi.org/10.1016/b978-0-443-15717-2.00010-x.
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Zhu, Jinyi, Haoran Meng, and Yan Li. "Screening and Bioinformatics Analysis of Differential Genes in Autism Spectrum Disorder Based on GEO Database." In Studies in Health Technology and Informatics. IOS Press, 2023. http://dx.doi.org/10.3233/shti230851.
Повний текст джерелаАнотація:
Objective: The prevalence of autism spectrum disorder (ASD) in children has been increasing year by year, which has seriously affected the quality of life of children. There are many theories about the cause of ASDs, with some studies suggesting that it may be related to gene expression levels or inflammation and immune system dysfunction. But the exact mechanism is not fully understood. Methods: profile of gene expression The protein interaction network (PPI) of differentially expressed genes was created using the STRING web tool and GSE77103, which was chosen from the gene expression omnibus (GEO) database. Using the CytoHubba plugin of Cytoscape program, the hub genes were examined. The hub gene regulatory network for miRNA-mRNA was then built. Results: We identified 551 differentially expressed genes(DEGs) in 8 children with ASD and normal children. In addition, we screened out 10 hub genes (MX1, ISG15, IRF7, DDX58, IFIT1, BCL2L1, HPGDS, CTSD, PTGS2 and CD68) that were most associated with the development of ASDs. Then, microRNAs (miRNAs) closely related to hub genes (such as has-miR-27a-5p) were screened, and the miRNA-mRNA regulatory network was constructed. Conclusion: In this study, a total of 10 hub genes were identified, including MX1, ISG15, IRF7, DDX58, IFIT1, BCL2L1, HPGDS, CTSD, PTGS2 and CD68, which are closely related to ASD. These genes may play a key role in the occurrence and progression of ASD. In addition, we also revealed some miRNAs that regulate the hub genes of ASD. These results may deepen our understanding of ASD and provide potential biomarkers and targets for future treatment of patients with ASD.
Тези доповідей конференцій з теми "MiRNA-mRNA interactions"
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Ferdous, Rayhan, Md Zahidul Islam, and Ferdous Bin Ali. "Identifying miRNA-mRNA interactions by a combination of spearman's rank correlation and IDA." In 2016 International Conference on Informatics, Electronics and Vision (ICIEV). IEEE, 2016. http://dx.doi.org/10.1109/iciev.2016.7760131.
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Guo, Yichen, Marie Denis, Rency S. Varghese, Sidharth S. Jain, Mahlet G. Tadesse, and Habtom W. Ressom. "Bayesian Approach Integrating Prior Knowledge for Identifying miRNA-mRNA Interactions in Hepatocellular Carcinoma." In 2023 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2023. http://dx.doi.org/10.1109/bibm58861.2023.10385314.
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Weidner, Julie, Carina Malmhäll, Aidan Barrett, Huda Hasan, Emma Boberg, Linda Ekerljung, and Madeleine Rådinger. "Glucocorticoid signaling genes are altered in asthma subgroups – A potential role for mRNA-miRNA interactions." In ERS International Congress 2020 abstracts. European Respiratory Society, 2020. http://dx.doi.org/10.1183/13993003.congress-2020.2715.
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Romero-Cordoba, Sandra L., Rosa Rebollar-Vega, Valeria Quintanar-Jurado, Alfredo Hidalgo-Miranda, Sergio Rodriguez-Cuevas, Veronica Bautista-Pina, and Antonio Maffuz-Aziz. "Abstract 4370: miRNA profiles identify different subgroups of triple negative tumors and reveal novel miRNA-mRNA interactions in breast cancer tumorigenesis." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-4370.
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Szczepankiewicz, D., W. Langwiński, J. Nowakowska, P. Kołodziejski, E. Pruszyńska-Oszmałek, M. Sassek, N. Leciejewska, K. Ziarniak, and A. Szczepankiewicz. "Allergic airway inflammation affects signaling pathways in adipose tissue via mRNA-miRNA interactions in the rat." In ERS International Congress 2022 abstracts. European Respiratory Society, 2022. http://dx.doi.org/10.1183/13993003.congress-2022.2275.
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Telonis, Aristeidis G. "Abstract B79: Integrative quantitative analysis of pancreatic ductal adenocarcinoma mRNA, miRNA, and methylation profiles reveals interactions that are dependent on tumor cellularity." In Abstracts: AACR Special Conference on Pancreatic Cancer: Advances in Science and Clinical Care; May 12-15, 2016; Orlando, FL. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.panca16-b79.
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Gabriel, Binthiya Suny, and Tessamma Thomas. "Novel Method for Analyzing the Relation of miRNA Seed Length and the miRNA-mRNA Interaction Strength." In 2021 Seventh International conference on Bio Signals, Images, and Instrumentation (ICBSII). IEEE, 2021. http://dx.doi.org/10.1109/icbsii51839.2021.9445143.
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YURIKOVA, O., S. ATAMBAYEVA, R. NIYAZOVA, and A. IVASHCHENKO. "INTERACTION OF MIRNA WITH MRNA OF ORTHOLOGOUS GENES INVOLVED IN THE DEVELOPMENT OF NEURODEGENERATIVE AND ONCOLOGICAL DISEASES." In 5TH MOSCOW INTERNATIONAL CONFERENCE "MOLECULAR PHYLOGENETICSAND BIODIVERSITY BIOBANKING". TORUS PRESS, 2018. http://dx.doi.org/10.30826/molphy2018-40.
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"Characteristics of interaction of miRNA with mRNA of C2H2, ERF and GRAS transcription factors of arabidopsis, rice and maize." In Bioinformatics of Genome Regulation and Structure/ Systems Biology. institute of cytology and genetics siberian branch of the russian academy of science, Novosibirsk State University, 2020. http://dx.doi.org/10.18699/bgrs/sb-2020-214.
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