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Статті в журналах з теми "MiR486"
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Karagun, Barbaros Sahin, Bulent Antmen, Ilgen Sasmaz, Kahraman Tanriverdi, Gulsum Ucar, and Yurdanur Kilinc. "Micro-RNA Profile of Childhood Acute Lymphoblastic Leukemia." Blood 120, no. 21 (November 16, 2012): 4824. http://dx.doi.org/10.1182/blood.v120.21.4824.4824.
Повний текст джерелаАнотація:
Abstract Abstract 4824 Micro-RNAs are functional, non-protein coding RNA molecules and their transcriptions provided by intron or exon regions of the genome and non-protein coding regions of RNA genes. The role of micro-RNAs in acute leukemia has become the subject of research increasingly. In this study we aimed to identify micro-RNA profiles in the childhood acute leukemia that one of hematologic malignancies. Fourty nine patients who were diagnosed with acute leukemia and admitted to Cukurova University Faculty of Medicine Department of Pediatric Hematology between December 2010 and September 2011. Blood samples were taken twice in patient groups at diagnosis and during remission and plasma samples were stored. Blood samples were taken once in the healthy group and plasma were separated. The plasma samples were investigated by PCR analysis of micro-RNA. Acute leukemia was diagnosed by cytomorphological, immuno histochemical and flow cytometric studies. Thirtyone patients who were diagnosed with ALL and fortyseven healthy children as a control group were included to study. miR20a, miR25, miR92a, miR30c, miR106b, miR203, miR150, miR192, miR302c, miR184, miR218, miR320, miR342-3p, miR223, miR328, miR483-5p, miR376a, miR381, miR451, miR576-3p, miR548a levels were increased in newly diagnosed ALL patients when compared to healthy controls (p <0.05). The miR20b, miR342-3p and miR548a levels were found higher in healthy controls than the newly diagnosed ALL patients group (p <0.05) Healthy control groups when compared with pediatric ALL patients whose in remission; miR769-3p, miR20a, miR92a, miR16, miR27b, miR192, miR320, miR223, miR484, miR451 levels were found higher in healthy control groups than the patients. Newly diagnosed pediatric ALL patients compared with patients whose in remission; miR30c, miR106b, miR25, miR184, miR218, miR302c, miR483-5p levels were increased in newly diagnosed pediatric ALL patients than ALL patients whose in remission (p<0.05). miRNAs are thought to be identified at a different level of expression in normal and pathological tissues can be determined between the miRNAs that are effective diagnosis and treatment of human cancers. We showed the microRNA profils that may play new roles treatment of acute leukemia in the futures. Disclosures: Kilinc: ApoPharma Inc.: Research Funding.
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Cao, Chunyu, Ruicai Long, Tiejun Zhang, Junmei Kang, Zhen Wang, Pingqing Wang, Hao Sun, Jie Yu, and Qingchuan Yang. "Genome-Wide Identification of microRNAs in Response to Salt/Alkali Stress in Medicago truncatula through High-Throughput Sequencing." International Journal of Molecular Sciences 19, no. 12 (December 17, 2018): 4076. http://dx.doi.org/10.3390/ijms19124076.
Повний текст джерелаАнотація:
Saline-alkaline stress is a universal abiotic stress that adversely affects plant growth and productivity. Saline-alkaline conditions results in plant abnormal transcriptome expression finally manifesting as defective phenotypes. Considerable research has revealed the active role of microRNA in various stress conditions. This study was aimed to identify novel miRNAs and the miRNA expression patterns in the leguminous model plant R108 (Medicago truncatula). The miRNA contained in the total RNA extracted from Medicago truncatula seedlings (72 h) that had been treated with solutions mimicking saline and alkaline soils was subjected to miRNA deep sequencing. The Illumina HiSeq sequencing platform was used to analyze nine small RNA libraries of three treatment groups: distilled water, 20 mM NaCl + Na2SO4 and 5 mM Na2CO3 + NaHCO3. Sequencing revealed that 876 miRNAs including 664 known miRNAs and 212 potential novel miRNAs were present in all the libraries. The miR159 family, miR156 family, miR2086-3p, miR396, miR166, miR319, miR167, miR5213-5p, miR1510 and miR2643 were among the most expressed miRNAs in all libraries. The results of miRNAs expression under treatments were validated by reverse-transcription quantitative PCR (RT-qPCR). Target gene prediction through computational analysis and pathway annotation analysis revealed that the primary pathways affected by stress were related to plant development, including metabolic processes, single-organism processes and response to the stimulus. Our results provide valuable information towards elucidating the molecular mechanisms of salt/alkali tolerance in Medicago truncatula and provide insight into the putative role of miRNAs in plant stress resistance.
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Narducci, M. G., D. Arcelli, M. C. Picchio, C. Lazzeri, E. Pagani, F. Sampogna, E. Scala, et al. "MicroRNA profiling reveals that miR-21, miR486 and miR-214 are upregulated and involved in cell survival in Sézary syndrome." Cell Death & Disease 2, no. 4 (April 2011): e151-e151. http://dx.doi.org/10.1038/cddis.2011.32.
Повний текст джерела
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Cao, Huiying, Xinyue Zhang, Yanye Ruan, Lijun Zhang, Zhenhai Cui, Xuxiao Li, and Bing Jia. "miRNA expression profiling and zeatin dynamic changes in a new model system of in vivo indirect regeneration of tomato." PLOS ONE 15, no. 12 (December 17, 2020): e0237690. http://dx.doi.org/10.1371/journal.pone.0237690.
Повний текст джерелаАнотація:
Callus formation and adventitious shoot differentiation could be observed on the cut surface of completely decapitated tomato plants. We propose that this process can be used as a model system to investigate the mechanisms that regulate indirect regeneration of higher plants without the addition of exogenous hormones. This study analyzed the patterns of trans-zeatin and miRNA expression during in vivo regeneration of tomato. Analysis of trans-zeatin revealed that the hormone cytokinin played an important role in in vivo regeneration of tomato. Among 183 miRNAs and 1168 predicted target genes sequences identified, 93 miRNAs and 505 potential targets were selected based on differential expression levels for further characterization. Expression patterns of six miRNAs, including sly-miR166, sly-miR167, sly-miR396, sly-miR397, novel 156, and novel 128, were further validated by qRT-PCR. We speculate that sly-miR156, sly-miR160, sly-miR166, and sly-miR397 play major roles in callus formation of tomato during in vivo regeneration by regulating cytokinin, IAA, and laccase levels. Overall, our microRNA sequence and target analyses of callus formation during in vivo regeneration of tomato provide novel insights into the regulation of regeneration in higher plants.
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Ding, Yueyun, Yinhui Hou, Zijing Ling, Qiong Chen, Tao Xu, Lifei Liu, Na Yu, et al. "Identification of Candidate Genes and Regulatory Competitive Endogenous RNA (ceRNA) Networks Underlying Intramuscular Fat Content in Yorkshire Pigs with Extreme Fat Deposition Phenotypes." International Journal of Molecular Sciences 23, no. 20 (October 20, 2022): 12596. http://dx.doi.org/10.3390/ijms232012596.
Повний текст джерелаАнотація:
Intramuscular fat (IMF) content is vital for pork quality, serving an important role in economic performance in pig industry. Non-coding RNAs, with mRNAs, are involved in IMF deposition; however, their functions and regulatory mechanisms in porcine IMF remain elusive. This study assessed the whole transcriptome expression profiles of the Longissimus dorsi muscle of pigs with high (H) and low (L) IMF content to identify genes implicated in porcine IMF adipogenesis and their regulatory functions. Hundreds of differentially expressed RNAs were found to be involved in fatty acid metabolic processes, lipid metabolism, and fat cell differentiation. Furthermore, combing co-differential expression analyses, we constructed competing endogenous RNAs (ceRNA) regulatory networks, showing crosstalk among 30 lncRNAs and 61 mRNAs through 20 miRNAs, five circRNAs and 11 mRNAs through four miRNAs, and potential IMF deposition-related ceRNA subnetworks. Functional lncRNAs and circRNAs (such as MSTRG.12440.1, ENSSSCT00000066779, novel_circ_011355, novel_circ_011355) were found to act as ceRNAs of important lipid metabolism-related mRNAs (LEP, IP6K1, FFAR4, CEBPA, etc.) by sponging functional miRNAs (such as ssc-miR-196a, ssc-miR-200b, ssc-miR10391, miR486-y). These findings provide potential regulators and molecular regulatory networks that can be utilized for research on IMF traits in pigs, which would aid in marker-assisted selection to improve pork quality.
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Ma, Jingyi, Pan Zhao, Shibiao Liu, Qi Yang, and Huihong Guo. "The Control of Developmental Phase Transitions by microRNAs and Their Targets in Seed Plants." International Journal of Molecular Sciences 21, no. 6 (March 13, 2020): 1971. http://dx.doi.org/10.3390/ijms21061971.
Повний текст джерелаАнотація:
Seed plants usually undergo various developmental phase transitions throughout their lifespan, mainly including juvenile-to-adult and vegetative-to-reproductive transitions, as well as developmental transitions within organ/tissue formation. MicroRNAs (miRNAs), as a class of small endogenous non-coding RNAs, are involved in the developmental phase transitions in plants by negatively regulating the expression of their target genes at the post-transcriptional level. In recent years, cumulative evidence has revealed that five miRNAs, miR156, miR159, miR166, miR172, and miR396, are key regulators of developmental phase transitions in plants. In this review, the advanced progress of the five miRNAs and their targets in regulating plant developmental transitions, especially in storage organ formation, are summarized and discussed, combining our own findings with the literature. In general, the functions of the five miRNAs and their targets are relatively conserved, but their functional divergences also emerge to some extent. In addition, potential research directions of miRNAs in regulating plant developmental phase transitions are prospected.
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Seyyedi, Samaneh Sadat, Masoud Soleimani, Marjan Yaghmaie, Monireh Ajami, Mansoureh Ajami, Shahram Pourbeyranvand, Kamran Alimoghaddam, and Seyed Mohammad Akrami. "Deregulation of miR-1, miR486, and let-7a in cytogenetically normal acute myeloid leukemia: association with NPM1 and FLT3 mutation and clinical characteristics." Tumor Biology 37, no. 4 (November 2, 2015): 4841–47. http://dx.doi.org/10.1007/s13277-015-4289-y.
Повний текст джерела
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Puchta, Marta, Jolanta Groszyk, Magdalena Małecka, Marek D. Koter, Maciej Niedzielski, Monika Rakoczy-Trojanowska, and Maja Boczkowska. "Barley Seeds miRNome Stability during Long-Term Storage and Aging." International Journal of Molecular Sciences 22, no. 9 (April 21, 2021): 4315. http://dx.doi.org/10.3390/ijms22094315.
Повний текст джерелаАнотація:
Seed aging is a complex biological process that has been attracting scientists’ attention for many years. High-throughput small RNA sequencing was applied to examine microRNAs contribution in barley seeds senescence. Unique samples of seeds that, despite having the same genetic makeup, differed in viability after over 45 years of storage in a dry state were investigated. In total, 61 known and 81 novel miRNA were identified in dry seeds. The highest level of expression was found in four conserved miRNA families, i.e., miR159, miR156, miR166, and miR168. However, the most astonishing result was the lack of significant differences in the level of almost all miRNAs in seed samples with significantly different viability. This result reveals that miRNAs in dry seeds are extremely stable. This is also the first identified RNA fraction that is not deteriorating along with the loss of seed viability. Moreover, the novel miRNA hvu-new41, with higher expression in seeds with the lowest viability as detected by RT-qPCR, has the potential to become an indicator of the decreasing viability of seeds during storage in a dry state.
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Salih, Haron, Wenfang Gong, Mtawa Mkulama, and Xiongming Du. "Genome-wide characterization, identification, and expression analysis of the WD40 protein family in cotton." Genome 61, no. 7 (July 2018): 539–47. http://dx.doi.org/10.1139/gen-2017-0237.
Повний текст джерелаАнотація:
WD40 repeat proteins are largely distributed across the plant kingdom and play an important role in diverse biological activities. In this work, we performed genome-wide identification, characterization, and expression level analysis of WD40 genes in cotton. A total of 579, 318, and 313 WD40 genes were found in Gossypium hirsutum, G. arboreum, and G. raimondii, respectively. Based on phylogenetic tree analyses, WD40 genes were divided into 11 groups with high similarities in exon/intron features and protein domains within the group. Expression analysis of WD40 genes showed differential expression at different stages of cotton fiber development (0 and 8 DPA) and cotton stem. A number of miRNAs were identified to target WD40 genes that are significantly involved in cotton fiber development during the initiation and elongation stages. These include miR156, miR160, miR162, miR164, miR166, miR167, miR169, miR171, miR172, miR393, miR396, miR398, miR2950, and miR7505. The findings provide a stronger indication of WD40 gene function and their involvement in the regulation of cotton fiber development during the initiation and elongation stages.
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Gao, Shiwu, Yingying Yang, Yuting Yang, Xu Zhang, Yachun Su, Jinlong Guo, Youxiong Que, and Liping Xu. "Identification of Low-Nitrogen-Related miRNAs and Their Target Genes in Sugarcane and the Role of miR156 in Nitrogen Assimilation." International Journal of Molecular Sciences 23, no. 21 (October 29, 2022): 13187. http://dx.doi.org/10.3390/ijms232113187.
Повний текст джерелаАнотація:
Chemical nitrogen (N) fertilizer is widely used in sugarcane production, especially in China and India. Understanding the molecular mechanisms and mining miRNAs and their target genes associated with nitrogen use efficiency (NUE) in sugarcane can aid in developing the N-efficient varieties, and thus is beneficial to reduce N fertilizer application. In this study, the root miRNA database of N-efficient sugarcane variety ROC22 under low N stress (0.3 mM NH4NO3) for 3 h was constructed, along with their transcriptome-rearranged data. KEGG analysis indicated that those candidate target genes, corresponding to differentially expressed miRNAs, were mainly enriched in N metabolism, amino acid metabolism, carbohydrate metabolism, photosynthesis, and hormone signal transduction pathways. It was found that under low N stress for 0–24 h, there was a negative correlation between miR168 and SPX, along with miR396 and acnA. Furthermore, the expression of miR156 in the roots of ROC22 was significantly up-regulated under low N treatment. Compared with the wild-type, the Arabidopsis plants overexpressing sugarcane miR156 exhibited significantly improved length and surface area of roots, while the expression of one NO3− transporter gene NRT1.1, three N assimilation key genes (NR1, NIR1, and GS), and the activity of two N assimilation key enzymes (NR and GS) were up-regulated under low N treatment. It can be reasonably deduced that sugarcane miR156 can enhance the nitrogen assimilation ability of the overexpressed Arabidopsis plants under low N application, and thus has a potential ability for improving sugarcane NUE. The present study should be helpful for understanding the molecular regulatory network in the N-efficient sugarcane genotype responding to low N stress and could provide the candidate miRNAs with a potential function in improving sugarcane NUE.
Дисертації з теми "MiR486"
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buonocore, sara. "The ANK1 rs508419-C T2D-risk allele increases the expression of sAnk1.5 and miR486 but their overexpression in transgenic mice does not significantly alter glucose tolerance." Doctoral thesis, Università di Siena, 2022. http://hdl.handle.net/11365/1211536.
Повний текст джерелаАнотація:
Genome wide association studies (GWAS) identified the ANK1 gene as a common type 2 diabetes
mellitus (T2D) susceptibility locus. More recently, GWAS studies identified a novel SNP
associated to T2D susceptibility, namely rs508419, in the internal promoter of ANK1 gene, which
drives the expression of small ankyrin 1.5 (sAnk1.5), a striated muscle-specific protein localized in
the sarcoplasmic reticulum (SR) membrane. sAnk1.5 interacts with the giant sarcomeric protein
obscurin, allowing the correct localization of SR around the myofibrils. The rs508419 SNP is
characterized by the substitution of a thymine with a cytosine, which determines an increased
transcriptional activity of the ANK1 internal promoter, resulting in high protein levels of sAnk1.5 in
skeletal muscle biopsies of individuals carrying the C/C variant of the SNP, with respect to
individuals carrying either T/T or C/T genotype.
Interestingly, the sequence of microRNA-486 (miR-486), a small non-coding RNA, is positioned in
the intron between exon 41 and exon 42 of the coding sequence of sAnk1.5. Therefore, miR-486 is
expressed under the transcriptional control of both the principal and of the internal promoters of
ANK1. miR-486 plays a role in the regulation of the PI3K/AKT signalling pathway, which
regulates several cellular processes, such as growth, cellular proliferation and survival, protein
synthesis and degradation, lipid and glucose metabolism.
In our laboratory we analysed human skeletal muscle biopsies to evaluate whether miR-486
expression levels were increased in individuals carrying the SNP rs508419. These results showed a
significant 3-fold increase of miR-486 expression levels in the skeletal muscle of individuals
carrying the C/C variant of the SNP compared to those carrying the T/T variant of the SNP.
Given that skeletal muscle is one of the main tissues involved in regulating glucose disposal, the
aim of this thesis was to verify whether sAnk1.5 and miR-486 overexpression in mouse skeletal
muscle might associate with T2D susceptibility. To this goal, we generated a double transgenic
mouse model, (TgsAnk1.5/+//TgmiR486/+), hereafter referred to as double Tg (D-Tg), where the sAnk1.5
coding sequence and the miR-486 sequence are under the transcriptional control of the skeletal
muscle-specific rat myosin light chain (MLC) promoter, and of the mouse muscle-specific creatine
kinase (CKM) promoter, respectively. Accordingly, the D-Tg mouse was expected to present a
skeletal muscle-specific increase of sAnk1.5 and miR-486 expression.
Indeed, RT-PCR experiments indicated that the expression of the transgene was restricted to striated
muscles and sAnk1.5 and miR-486 mRNAs levels were both robustly increased in transgenic mice
compared to WT. Accordingly, western blot analysis of protein extracts from gastrocnemius,
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extensor digitorum longus (EDL) and soleus muscles revealed an increase in sAnk1.5 protein levels
of 45%, 60% and 35%, respectively, with respect to control mice.
Notably, in the D-Tg mouse model we observed a significant discrepancy between the levels of
sAnk1.5 protein expression and the levels of the corresponding mRNA. Indeed, in the
gastrocnemius muscle, sAnk1.5 mRNA was about 12 times more expressed in transgenic mice,
compared to only 1.5-fold increase in protein expression, suggesting a post-translational regulation
of sAnk1.5 expression. Evidence of this was confirmed by muscular administration of the
proteasome inhibitor MG132 in transgenic mice. Results showed sAnk1.5 protein expression levels
significantly increased of about 35% compared to those observed in the contralateral untreated
gastrocnemius muscles.
To evaluate the potential association between sAnk1.5 and miR-486 overexpression with T2D,
glucose and insulin tolerance were monitored during a period of 12 months in male transgenic mice.
Blood glucose levels after overnight fasting were significantly higher in 2 and 6 months old
transgenic mice compared to WT controls. However, overall glucose and insulin tolerance was not
altered between the two experimental groups.
Finally, considering the miR-486 role in regulating PI3K/AKT signalling pathway, which in
skeletal muscle fiber is activated following insulin stimulation thus being a key pathway in
maintaining glucose homeostasis, we characterized the expression pattern of PTEN, p85α,
FOXO1a, pAKTser473, and GLUT4 in the skeletal muscles of D-Tg mice.
Currently, we are performing additional experiments where D-Tg mice are fed with a high-fat diet
to better evaluate whether sAnk1.5 and miR-486 overexpression might predispose to T2D
susceptibility.
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Yuan, Ke. "THE CHARACTERIZATION OF HSA-MIR148A IN HEPATOCARCINOGENESIS." Diss., Temple University Libraries, 2011. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/154268.
Повний текст джерелаАнотація:
BiologyPh.D.
Chronic Hepatitis B Virus (HBV) infection is a global health problem because of its connection to acute and chronic liver diseases as well as hepatocellular carcinoma (HCC). There is increasing evidence showing that HBV contributes to HCC due to persistently high levels of trans-activating protein---hepatitis B encoded x antigen (HBxAg). Studies have shown that the HBxAg affects and alters the activity of many different transcription factors and plays an essential role in several cytoplasmic signaling transduction pathways, such as Wnt signaling pathways. One of the upregulated genes, designated URG11, was found transactivated by HBxAg. URG11 could stimulate the ß-catenin promoter and hepatocellular growth and survival which suggest that URG11 may be a regulatory element in the ß-catenin signaling pathways. microRNA148a (miR148a) was identified from two miRNA microarrays as one of the up-regulated miRNAs in cells stably expressing HBxAg or over-expressing URG11. Moreover, the expression of miR148a was also elevated in HBV-mediated HCC patient tissue samples. To study the function of miR148a, HepG2 (hepatoblastoma) and Hep3B (hepatoma) cells stably expressing HBxAg or over-expressing URG11 were transduced by recombinant lentiviruses encoding anti-miR148a. anti-miR148a suppressed cell proliferation, cell cycle progression, cell migration, anchorage independent growth in soft agar and subcutaneous tumor formation in SCID mice. Further, introduction of anti-miR148a increased PTEN protein and mRNA expression, suggesting that PTEN was suppressed by miR148a. In addition, anti-miR148a blocked the stimulation of Akt signaling, resulting in decreased expression of ß-catenin. Thus, miR148a may play a central role in HBxAg/URG11 mediated HCC, and may be an early diagnostic marker and/or therapeutic target associated with this tumor type.
Temple University--Theses
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Rojas, Carlos Barrera. "The role of the microRNA156/SPL pathway during the primary root growth of Arabidopsis thaliana." Botucatu, 2019. http://hdl.handle.net/11449/181164.
Повний текст джерелаАнотація:
Orientador: Fábio Silveira NogueiraResumo: O sistema radicular (SR) é importante pela ancoragem e obtenção de água e nutrientes. Em eudicotiledôneas, como Arabidopsis, o crescimento da raiz primária (RP) é afetado por fitormônios, especialmente pelo balanço entre auxina que controla a divisão celular, e citocinina que modula a diferenciação celular; também, os microRNAs (miRNAs), um sub-conjunto de pequenos RNAs que regulam pós-transcricionalmente seus alvos, regulam o crescimento da RP. O microRNA156 (miR156) e seus alvos, membros da família SQUAMOSA Promoter-Binding Protein-Like (SPL), constituem uma via genética que regula vários processos do desenvolvimento, incluindo desenvolvimento da raíz; porém, durante o crescimento da RP, não foi observado o efeito da via miR156/SPL, e da interação com auxina e citocinina; assim, foi avaliada essa interação durante o crescimento da PR regulado pelo tamanho do meristema da raiz (TMR) em Arabidopsis. Usando ferramentas genéticas e moleculares foi analizada a expressão de genes MIR156 e SPLs, o comprimento da RP, o TMR, as taxas de divisão celular, e as respostas de auxina e citocinina durante o crescimento da RP. Os genes MIR156 e SPLs possuem padrões de expressão opostos. Níveis altos do miR156 (nas plântulas p35S :: MIR156A), leva a menor comprimento da RP, TMR reduzido, menores taxas de divisão celular, respostas mais baixas e altas à auxina e citocinina respectivamente; em contraste, níveis severamente reduzidos do miR156 maduro disponível (nas plantas MIM156) conducem a e... (Resumo completo, clicar acesso eletrônico abaixo)
Doutor
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Souza, Felipe Herminio Oliveira. "Caracterização funcional do módulo miR156/SlSBP6c no desenvolvimento do tomateiro (Solanum lycopersicum L.)." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/11/11144/tde-21032019-141310/.
Повний текст джерелаАнотація:
A genética molecular permite o entendimento dos mecanismos que regulam o desenvolvimento dos órgãos vegetais em resposta a fatores bióticos e abióticos. A regulação de vias gênicas é de fundamental importância no sucesso reprodutivo, evolutivo e econômico dos vegetais. Uma das modalidades de regulação é a pós transcricional através de microRNAs (miRNAs). Tratam-se de pequenos RNAs endógenos não codantes que possuem uma complementaridade quase perfeita em plantas. Em plantas, muitos genes-alvos de miRNA codificam-se para fatores de transcrição, como os genes SQUAMOSA Promoter-Binding Protein-Like (SPL/SBP). O microRNA156, conservado entre as Angiospermas, regula diversos fatores de transcrição da família SBP. O módulo miR156/SBP, denominado via da idade (AGE), atua ao longo do ciclo de vida dos vegetais regulando as transições de fase: juvenil-adulta e vegetativa-reprodutiva. O tomateiro, Solanum lycopersicum L., possui genes SlSBPs regulados pelo miR156 e com funcionalidade não esclarecida. Dentre aqueles, o gene SlSBP6c (Solyc12g038520) se destaca por possuir maior similaridade filogenética com SPL6s/SBP6s de solanáceas do que seus genes homólogos SlSBP6a e SlSBP6b o que sugere a hipótese de um possível ganho de função. Com o intuito de testar essa hipótese, o presente trabalho objetivou caracterizar funcionalmente o gene SlSBP6c. Para tanto, utilizou-se o genótipo 35S::rSlSBP6c, planta transformada de tomateiro cultivar Micro-Tom (MT) que super-expressa a versão resistente ao miR156 do gene SlSBP6, fusionada ao promotor viral 35S. Este material vegetal foi gerado pelo laboratório de Genética Molecular do Desenvolvimento Vegetal e cedido para execução desta pesquisa. O trabalho se desenvolveu em duas etapas: caracterização molecular e morfo-fisiológica. Primeiramente, os genes SlSBP6a, SlSBP6b e SlSBP6c de tomateiro foram analizados quanto a sua expressão ao longo do desenvolvimento em folhas e inflorescência. E, posteriormente, foram analisados a complexidade foliar, o tempo de florescimento, a transição do meristema vegetativo para o reprodutivo, o teor relativo de clorofila e a fotossíntese líquida. Os resultados obtidos demonstram que os genes SlSBP6a e SlSBP6c são semelhantes no padrão de expressão gênica na homologia das protéinas que o codificam, indicando similaridade funcional. A de-regulação do gene SlSBP6c leva ao aumento a complexidade foliar, o teor relativo de clorofila e a fotossíntese líquida. O atraso na transição do meristema vegetativo para o reprodutivo se evidencia por um florescimento tardio nas plantas que super-expressam o gene SlSBP6c. Os resultados demonstram que o gene SlSBP6c exerce funcionalidade no tomateiro atuando na transição de fase vegetativo-reprodutivo.Molecular genetics allows the understanding of the mechanisms that regulate the development of plant organs in response to biotic and abiotic factors. The regulation of gene pathways is of fundamental importance in the reproductive, evolutionary and economic success of the plants. Research has been increasing the knowledge about post-transcriptional regulation through microRNAs (miRNAs). The miRNAs are small non-coding endogenous RNAs that have almost perfect complementarity in plants. Many miRNA target genes in plants are encoded by transcription factors, such as the SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL / SBP) genes. MicroRNA156 is conserved among Angiosperms and regulates several transcription factors of the SBP family. The miR156 / SBP module, called the age pathway (AGE), acts throughout the life cycle of plants regulating phase transitions juvenile-adult and vegetative-reproductive. The tomato, Solanum lycopersicum L., has newly described and unintelligently regulated miR156 regulated SlSBPs. Among these, the gene SlSBP6c (Solyc12g038520) stands out for having greater phylogenetic similarity with solanaceous SBP6s than its homologous genes SlSBP6a and SlSBP6b, indicating a possible gain of function related to characteristics of this family of plants as fleshy fruits and composite leaves. In order to test this hypothesis the work aimed to characterize the SlSBP6c gene functionally. Using as a tool the 35S::rSlSBP6c genotype, transformed tomato plant micro-Tom (MT) that over-expresses the miR156 resistant version of the SlSBP6 gene, fused to the 35S viral promoter. The Laboratory of Molecular Genetics of Plant Development generated this plant material. The work was developed in two stages: molecular and morpho-physiological characterization. In the first the genes SlSBP6a, SlSBP6b and SlSBP6c of tomato were analyzed for their expression throughout the development in leaves and inflorescence. In the second, the leaf complexity, the flowering time, the transition from vegetative to the reproductive meristem, the relative chlorophyll content and the net photosynthesis were evaluated. The results obtained demonstrate that the SlSBP6a and SlSBP6c genes are very similar in the pattern of gene expression in the homology of the coding proteins, indicating a functional similarity. The SlSBP6c gene acts to increase leaf complexity, relative chlorophyll content and liquid photosynthesis. A late flowering in plants that overexpress the SlSBP6c gene evidences the delay in the transition from the vegetative to the reproductive meristem. The data set demonstrates that the SlSBP6c gene has important functionality in tomatoes acting on vegetative-reproductive phase transition.
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Mantovanini, Luana Jandhy [UNESP]. "Seleção de genótipos, análises fisiológicas e expressão de miRNAs em cana-de-açúcar (Saccharum spp.) na resposta ao alumínio." Universidade Estadual Paulista (UNESP), 2017. http://hdl.handle.net/11449/151477.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
A cana-de-açúcar é atualmente uma das principais culturas da agroindústria mundial. Devido à ampla expansão de seu plantio é submetida constantemente a solos não produtivos. A presença de moléculas tóxicas no solo, como o alumínio (Al3+), interfere diretamente no desenvolvimento radicular ocasionando baixa absorção de água e nutrientes levando a pouca produtividade e desenvolvimento das plantas. Os microRNAs tem sido descritos como um dos fatores responsáveis pela regulação gênica e a descoberta dessas moléculas abre um novo caminho para a elucidação da tolerância e adaptação das plantas aos estresses abióticos. Este estudo visou avaliar em duas cultivares de cana-de-açúcar (CTC-2 e RB855453) a expressão dos microRNAs miR159, miR164 e miR168, associados à resposta ao alumínio em espécies como Arabidopsis thaliana, arroz (Oriza sativa) e tabaco (Nicotiana tabacum). Algumas características foram avaliadas, como densidade de raízes (DS), área foliar (AR), produção de massa seca (MS) e teor de prolina nas folhas, em quatro cultivares de cana-de-açúcar submetidas a diferentes concentrações de alumínio. A cultivar CTC 2 foi classificada como tolerante e a RB855453 como sensível ao estresse. Ambas foram selecionadas e em sistema de hidroponia submetidas novamente ao estresse pela toxidez de alumínio na concentração de 221 μmol L -1 . Parâmetros fisiológicos foram mensurados (área foliar, potencial osmótico, taxa de fotossíntese, transpiração, condutância estomática) e a expressão dos miRNAs 159, 164 e 168 avaliada por PCR em tempo real. Plantas das duas cultivares apresentaram alterações fisiológicas e morfológicas ao longo do estresse, com redução significativa para a área foliar da cultivar RB855453. O miR164 e 159 foram induzidos nas duas cultivares, principalmente após 72 horas de estresse, e o miR168 diferencialmente expresso. Esses miRNAs regulam genes e fatores de transcrição que estão envolvidos na resposta e desenvolvimento da planta diante ao estresse por alumínio.
The sugarcane is currently one of the main crops of global agribusiness. Due to the wide expansion of its plantation is constantly subjected unproductive soils. The presence of toxic molecules in the soil, such as aluminum (Al3+), directly affects root development, leading to poor absorption of nutrients and water leading to low productivity and development of plants. Studies of the interactions of plants with the environment are being conducted to clarify the resistance or susceptibility of various cultures, favoring the discovery of important mechanisms that participate in physiological and molecular responses to environmental stresses. MicroRNAs have been described as one of the factors responsible for gene regulation and the discovery of these molecules opens a new path for the elucidation of tolerance and adaptation of plants to abiotic stresses. This study evaluated in two sugarcane varieties the expression of microRNA miR159, miR164 and miR168, associated with the response to the aluminum species such as Arabidopsis thaliana, rice (Oryza sativa) and tobacco (Nicotiana tabacum). Some characteristics were evaluated, such as density (DS), area (AR), dry mass production (DM) and proline content, in four sugarcane cultivars submitted to different concentrations of aluminum. CTC 2 cultivar was classified as tolerant and RB855453 as stress sensitive. Both were selected and in a hydroponics system again submitted to stress by the aluminum toxicity in the concentration of 221 μmol L-1. Physiological parameters were measured (leaf area, osmotic potential, photosynthesis rate, transpiration, stomatal conductance) and the expression of miRNAs 159, 164 and 168 evaluated by real-time PCR. Plants of both cultivars presented physiological and morphological changes along the stress, with a significant reduction for the leaf area of cultivar RB855453. The miR164 and 159 were induced in the two cultivars, mainly after 72 hours of stress, and the miR168 differentially expressed. These miRNAs regulate genes and transcription factors that are involved in the response and development of the plant in the face of aluminum stress.
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Rocha, Gabriel Henrique Braga. "Análise do papel da via miR156/SQUAMOSA Promoter-Binding Protein-Like (SPL) na organogênese in vitro a partir de raízes de Arabidopsis thaliana." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11144/tde-17062016-180648/.
Повний текст джерелаАнотація:
Os microRNAs (miRNAs) são pequenos RNAs endógenos não codantes de 21-24 nucleotídeos (nt) que regulam a expressão gênica de genes-alvos. Eles estão envolvidos em diversos aspectos de desenvolvimento da planta, tanto na parte aérea, quanto no sistema radicular. Entre os miRNAs, o miRNA156 (miR156) regula a família de fatores de transcrição SQUAMOSA Promoter-Binding Protein-Like (SPL) afetando diferentes processos do desenvolvimento vegetal. Estudos recentes mostram que a via gênica miR156/SPL apresenta efeito positivo tanto no aumento da formação de raízes laterais, quanto no aumento de regeneração de brotos in vitro a partir de folhas e hipocótilos em Arabidopsis thaliana. Devido ao fato de que a origem da formação de raiz lateral e a regeneração in vitro de brotos a partir de raiz principal compartilham semelhanças anatômicas e moleculares, avaliou-se no presente estudo se a via miR156/SPL, da mesma forma que a partir de explantes aéreos, também é capaz de influenciar na regeneração de brotos in vitro a partir de explantes radiculares. Para tanto foram comparados taxa de regeneração, padrão de distribuição de auxina e citocinina, análises histológicas e histoquímicas das estruturas regeneradas em plantas com via miR156/SPL alterada, incluindo planta mutante hyl1, na qual a produção desse miRNA é severamente reduzida. Além disso, foi avaliado o padrão de expressão do miR156 e específicos genes SPL durante a regeneração de brotos in vitro a partir da raiz principal de Arabidopsis thaliana. No presente trabalho observou-se que a alteração da via gênica miR156/SPL é capaz de modular a capacidade de regeneração de brotos in vitro a partir de raiz principal de Arabidopsis thaliana e a distribuição de auxina e citocinina presente nas células e tecidos envolvidos no processo de regeneração. Plantas superexpressando o miR156 apresentaram redução no número de brotos regenerados, além de ter o plastochron reduzido quando comparado com plantas controle. Adicionalmente, plantas contento o gene SPL9 resistente à clivagem pelo miR156 (rSPL9) apresentaram severa redução na quantidade de brotos, além de terem o plastochron alongado. Interessantemente, plantas mutantes hyl1-2 e plantas rSPL10 não apresentaram regeneração de brotos ao longo da raiz principal, mas sim intensa formação de raízes laterais e protuberâncias, respectivamente, tendo essa última apresentado indícios de diferenciação celular precoce. Tomados em conjunto os dados sugerem que o miR156 apresenta importante papel no controle do processo de regeneração de brotos in vitro. Entretanto, esse efeito é mais complexo em regeneração in vitro a partir de raízes do que a partir de cotilédones ou hipocótilos.MicroRNAs (miRNAs) are endogenous small non-coding RNAs of 21-24 nucleotides (nt) in length that regulate target gene expression. They are involved in many aspects of plant development, both in the shoot and in the root systems. Among miRNAs, miRNA156 (miR156) regulates SQUAMOSA Promoter Binding-Like (SPL) transcription factor family affecting different plant development processes. Recent studies have shown that the miR156/SPL pathway has a positive effect both in the increase of lateral root formation and regeneration of shoots from leaves and hypocotyls in Arabidopsis thaliana. Because the origin of lateral root formation and in vitro shoot regeneration from primary root share similar anatomical and molecular features, in the present study was evaluated whether the miR156/SPL pathway, in the same manner that from aerial explants, is also able to influence the in vitro shoot regeneration from root explants. For this, it was compared regeneration rates, distribution pattern of auxin and cytokinin, histological and histochemical analyses of the structures regenerated in plants in with the miR156/SPL pathway is modified, including the mutant hyl1-1, in which the biosynthesis of this miRNA is severely reduced. Besides that, it was evaluated the expression pattern of miR156 and specific SPL target genes during in vitro shoot regeneration from primary roots of Arabidopsis it was observed that the alteration on the miR156/SPL pathway is capable to modulate in vitro shoot regeneration from the primary root of Arabidopsis and the distribution of auxin and cytokinin at the tissues and cells involved in the regeneration process. Plants overexpressing the miR156a have shown reduction in the number of regenerated shoots, and displayed a reduction in plastochron when compared with wild type plants. Additionally, plants expressing cleavage-resistant form of SPL9 (rSPL9) presented severe reduction in the amount of shoots, and extended plastochron. Interestingly, mutant hyl1-2 and plants rSPL10 did not show any shoot regeneration along the root, but high formation of lateral roots and protuberances, respectively, having rSPL10 presented evidence of precocious cell differentiation. Taken together, these data suggest that de miR156 and SPLs have an important role in the control the in vitro shoot regeneration process. However, its effect is somehow more complex in roots than in cotyledons or hypocotyls.
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Jovanovic, Mariana. "MicroARN : acteurs de l'architecture racinaire et de la réponse aux contraintes environnementales chez les plantes ?" Paris 11, 2008. http://www.theses.fr/2008PA112078.
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Les petits ARN non codant et particulièrement les microARN ont été récemment impliqués comme régulateurs post-transcriptionnels du développement et de la réponse aux stress chez les plantes. Les travaux présentés ici avaient pour but d’identifier et de caractériser de nouveaux petits ARN qui pourraient intervenir dans la réponse aux contraintes environnementales et/ou la plasticité de l’architecture racinaire. L’analyse d’une banque de petits ARN, construite à partir de cultures cellulaires d’Arabidopsis thaliana traitées au H2O2, a permis d’identifier 51 nouvelles séquences dont certaines présentent des caractéristiques particulières et/ou des domaines d’expression différents dans la plante ou qui pourraient être liés au stress oxydatif. Des cibles potentielles de ces petits ARN ont été identifiées. De plus, nous avons identifié et caractérisé un microARN spécifique d’Arabidopsis, le MIR773, s’accumulant préférentiellement dans les racines. Il cible certaines protéines de la famille MET, qui sont les homologues végétaux de la cytosine ADN méthyltransférase majeure chez les animaux (Dnmt1). Nous avons généré des lignées transgéniques qui surexpriment ce MIR et analysé en détail leur développement ainsi que leurs profils de méthylation. Toutefois, nos données indiquent que ce couple microARN/cible n’interviendrait pas dans la régulation de la méthylation de l’ADN. Enfin, nous décrivons le rôle du gène MtMIR166a, qui contient deux copies en tandem du MIR166, dans la régulation de plusieurs facteurs de transcription de type class III HD-ZIP (HomeoDomain leucine-ZIPper) intervenant dans le développement des nodules et des racines secondaires chez Medicago truncatulaSmall non coding RNAs, and particularly microRNAs, have been lately implicated as post-transcriptional regulators of several developmental processes and stress responses in plants. This project aimed to identify and characterize new small RNAs that could be involved in responses to environmental constraints and/or linked to the root adaptative plasticity. Using a small RNA library constructed from Arabidopsis thaliana cell cultures treated with H2O2, we identified 51 new small RNA sequences. Among them, several displayed particular characteristics and/or expression patterns in plant tissues or in response to oxidative stress. Potential targets were identified. Furthermore, we identified and characterized one Arabidopsis-specific microRNA, MIR773, whose expression is enriched in root tissues. MIR773 targets a subset of the MET family proteins, which are plant homologues of the major DNA cytosine methyltransferase in mammals (Dnmt1) and plants (MET1). We generated transgenic lines overexpressing this microRNA and analyzed in detail their phenotypes during development and in response to abiotic stresses. Finally, we attempted to analyze the methylation profiles of these lines. However, results indicate that this microRNA/target pair may not be involved in the regulation of DNA methylation in plants. Finally, we describe the involvement of the MtMIR166a locus, which contains two tandem copies of mature MIR166, in the regulation of several class III HD-ZIP (HomeoDomain leucine-ZIPper) genes in Medicago truncatula. Particularly, this microRNA has been involved in the regulation of symbiotic nodule and lateral root development
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Alter, Christina [Verfasser], Wiebke [Akademischer Betreuer] Hansen, and Ralf [Akademischer Betreuer] Küppers. "Einfluss der Überexpression von CD83 und miR183 in CD4+ T-Zellen / Christina Alter. Gutachter: Wiebke Hansen ; Ralf Küppers. Betreuer: Wiebke Hansen." Duisburg, 2014. http://d-nb.info/1055906967/34.
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Silva, Geraldo Felipe Ferreira e. "A inter-relação entre a via miR156/SBP e o fitormônio giberelina no controle da transição de fase vegetativo-reprodutivo em tomateiro." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-11112016-105807/.
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O florescimento é um processo chave no desenvolvimento vegetal. A mudança de identidade do meristema apical de vegetativo para reprodutivo desencadeia reprogramação genética com efeitos em todo o corpo vegetal. Arabidopsis thaliana é conhecida como o principal modelo de estudo para esse processo apresentando até o momento cinco principais vias genéticas regulatórias. Tais vias apresentam redundância, sendo complexa a eliminação total da transição de fase nessa espécie. A via AGE, regulada pela idade da planta, tem como principais reguladores o mir156 e seus alvos diretos, os fatores de transcrição da família SPL/SBP (SQUAMOSA PROMOTER BINDING PROTEIN-like). Uma segunda via é controlada pelo fitohormônio giberelina (GA), o qual atua de maneira oposta em Arabidopsis thaliana (arabidopsis) e Solanum lycopersicum L. (tomateiro). Em tomateiro, diferentemente de arabidopsis, o cruzamento entre mutantes com conteúdo alterado de GA e plantas transgênicas superexpressando o miR156 (156OE; SILVA et al., 2014) demonstraram efeito sinérgico no atraso do tempo de florescimento. A aplicação de GA3 em plantas 156OE apresenta efeito similar aos cruzamentos citados sobre a transição do meristema apical. Em um dos cruzamentos entre mutantes da via GA e plantas 156OE, foi possível obter plantas apresentando completo bloqueio da transição de fase vegetativo-reprodutivo. A oferta extra do florígeno SINGLE FLOWER TRUSS (SFT) via enxertia não foi suficiente para restaurar a transição de fase nessas plantas, sugerindo que vias associadas à GA e AGE regulam alvos em comum, os quais podem ser independentes da regulação por SFT. Além disso, a regulação transcricional, e possivelmente pós-transcricional de alguns genes SBPs por diferentes vias associadas à GA, sugere uma complexa inter-relação entre as vias GA e AGE em tomateiro durante o florescimento. A ação combinada das vias GA e AGE foi capaz de inibir completamente o florescimento em tomateiro, regulação oposta ao verificado na planta modelo Arabidopsis thaliana. O efeito inibitório de GA sobre o florescimento é também visualizado em plantas lenhosas, sugerindo que as descobertas científicas realizadas em tomateiro podem ser expandidas para essas espécies, nas quais a experimentação é lenta e laboriosaThe flowering process is a major developmental event during the plant life cicle. The meristem identity switches from vegetative to reproductive, triggering substantial genetic modifications that affect the whole plant body. Arabidopsis thaliana is a major model for flowering with five different pathways controlling this process. These pathways are redundant, making complex the complete elimination of phase change in this species. One of the pathways is termed AGE since it is regulated by the time of development. The miR156 and its direct target SBP (SQUAMOSA PROMOTER BINDING PROTEIN-like) are the main regulators of the AGE pathway. A second pathway is controlled by the phytohormone gibberellin (GA), which acts in opposite ways when comparing Arabidopsis thaliana and tomato. In tomato, unlike Arabidopsis, the cross between mutants with altered contents of GA and transgenic plants overexpressing the miR156 (156OE; SILVA et al, 2014) showed synergistic effect in delayed flowering time. Treatments of GA3 in plants 156OE lead to similar effects visualized on the crosses above related to meristem transition. Among the crosses between GA mutants and 156OE plants, one double mutant could completely abolish the phase change in tomato. An extra offer of the florigen (SINGLE FLOWER TRUSS or SFT) by grafting experiments was unable to restore the flowering process in this double mutant. It suggests, pathways associated to GA and AGE regulate common downstream targets, which could be independent of SFT regulation. Moreover, the transcriptional regulation, and possible the post-transcriptionally regulation of some SBP targets by different pathways associated to GA, suggest a complex network between GA and AGE during the flowering in tomato. The combined action of GA and AGE pathways can complete impaired the flowering in tomato, this interaction is opposed to the model Arabidopsis thaliana. The negative effect of GA over the time of flowering is presented in wood plants, suggesting the scientific discoveries in tomato could be expanded to these species, which experiments are slow and laborious
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Corazon-Guivin, Mike Anderson [UNESP]. "Interação entre o gene TKN2 (KNOX-type I) e o miR156 node durante a transição de fase vegetativa para reprodutiva em tomateiro (Solanum lycopersicum)." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/110403.
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000785259.pdf: 441624 bytes, checksum: 15f9d5f63f3443d00a9a26ba9b0cd319 (MD5)O desenvolvimento das plantas depende da atividade de um grupo de células em divisão chamado de meristema. Extensas análises genéticas identificaram os principais reguladores do meristema apical vegetativo (SAM), os quais controlam o desenvolvimento de todos os órgãos aéreos. Dentre eles, há um grupo de homeoproteínas denominadas TALE (three-amino-acid-loop- extension); esta família contém os membros KNOTTED-like homeodomain (KNOX) e BELL-like Homeodomain (BELL), que funcionam como homodímeros ou heterodímeros, para regular a expressão de seus genes alvos mediante sua ligação à sequências especificas no DNA. Em plantas com folhas compostas como o tomateiro (Solanum lycopersicum), genes KNOX da classe I (KNOX I) são expressos no meristema, assim como também em folhas, flores e frutos, sugerindo que eles podem exercer várias funções nestes órgãos. Esta hipótese é corroborada pelos fenótipos intrigantes encontrados em mutantes com ganho de função dos genes KNOX I, cuja expressão ectópica afeta a forma da folha, pétala e frutos. Um exemplo é o tomateiro mutante Mouse ear (Me), que superexpressa o gene TKN2 (KNOX I). Fenótipos semelhantes também foram observados em plantas transgênicas superexpressando o microRNA156 (miR156). Os MicroRNAs são uma nova classe de pequenas moléculas de RNA não codantes (20-25 nucleotídeos) que se encontram amplamente distribuídos no genoma de plantas e animais, regulando a expressão de seus genes alvos principalmente ao nível pós-transcricional. O miR156 regula pós-transcricionalmente membros da família gênica do tipo SQUAMOSA Promoter-Binding Protein-Like (SPL ou SBP-box), os quais codificam fatores de transcrição específicos de plantas. Tais genes desempenham papéis importantes em diferentes aspectos do desenvolvimento. Para analisar a possível interação molecular entre o fator de transcrição TKN2 e a via microRNA156/SQUAMOS Promoter-Binding ...
Plant development depends on the activity of a group of dividing cells called meristem. Extensive genetic analyses have identified the major regulators of the shoot apical meristem (SAM), which control the development of all aerial organs. Among them, the three-amino-acid- loop-extension (TALE) class of homeoproteins; this family contains the KNOTTED-like homeodomain (KNOX) and BELL-like Homeodomain (BELL) members, which function as heterodimers or homodimers, to regulate expression of their target genes by binding to specific sequences in DNA. In plants with compound leaves as tomato (Solanum lycopersicum), KNOX I are expressed in the meristem, as well as on leaves, flowers and fruits, suggesting that they may play various roles in these organs. This hypothesis is supported by the intriguing phenotypes found in mutants with gain-of function of KNOX I genes, whose ectopic expression affects leaf, petal and fruit shape. An example, is the tomato mutant Mouse ear (Me), which overexpress the gene TKN2 (KNOX I). Similar phenotypes were also observed in transgenic plants overexpressing microRNA156 (miR156). MicroRNAs are a class of small no-coding RNAs (20-25 nucleotides) that are widely distributed in the genome of plants and animals, regulating the expression of their target genes by acting mainly at the post-transcriptional level. miR156 regulated post-transcriptionally most SQUAMOSA Promoter-Binding Protein-Like (SPL or SBP-box) genes, which encode plant-specific transcription factors. These genes play important roles in different aspects of development. To examine a possible molecular interaction between TKN2 transcription factor and microRNA156/SQUAMOSA Promoter-Binding Protein-Like module (miR156 node), it was evaluated the expression of miR156, its targets (SBP-box) and several genes downstream of miR156 node in different stages of the development of homozygous Me plants. Moreover, to evaluate the genetic interaction ...
Частини книг з теми "MiR486"
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Schwab, Rebecca. "Roles of miR156 and miR172 in Reproductive Development." In MicroRNAs in Plant Development and Stress Responses, 69–81. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-27384-1_4.
Повний текст джерела
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Schwab, Rebecca. "The Roles of miR156 and miR172 in Phase Change Regulation." In MicroRNAs in Plant Development and Stress Responses, 49–68. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-27384-1_3.
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Biswas, Priscilla, Eddi Di Marco, and Mauro S. Malnati. "A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PC R." In Methods in Molecular Biology, 55–64. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9833-3_5.
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Wang, Jia-Wei. "The Multifaceted Roles of miR156-targeted SPL Transcription Factors in Plant Developmental Transitions." In Plant Transcription Factors, 281–93. Elsevier, 2016. http://dx.doi.org/10.1016/b978-0-12-800854-6.00018-x.
Повний текст джерелаТези доповідей конференцій з теми "MiR486"
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Perez, Matheus Moreira, David Feder, Beatriz da Costa Aguiar Alves, Fernando Luiz Affonso Fonseca, and Alzira Alves de Siqueira Carvalho. "myoMIR and gene expression in myofibrillar myopathy." In XIII Congresso Paulista de Neurologia. Zeppelini Editorial e Comunicação, 2021. http://dx.doi.org/10.5327/1516-3180.662.
Повний текст джерелаАнотація:
Background: Myofibrillar myopathies (MFM) represent a heterogeneous group of muscle disorders caused by mutations in different genes. It has been identified a group of microRNAs present in muscles named myoMIR. Objective: Evaluate the diagnostic value of these myoMIRs and mRNA expression in skeletal tissue from muscle biopsy of patients with MFM. Design and Setting: Muscle biopsies from 16 MFM patients with mutations in Desmin (DES), Myotilin (MYOT), ZASP, or Filamin C (FLNC) genes, and 18 donors (patients with minimal non- specific changes in muscle biopsy) were included. Study were conducted at FMABC. Methods: mRNA and myoMIR expression from both groups were assessed. The target myoMIRs were MIR1, MIR133a, MIR133b, MIR206, MIR208a, MIR208b, MIR486, and MIR499. Anova and Student’s t-test were performed. Results: Six patients presented mutations in DES, five in ZASP, three in FLNC, and two in MYOT. MIR133b (p=0.05), MIR499 (p=0.027), and mRNA expression was up-regulated in patients with MFM. MIR208a (p=0.042) was higher in the control group. We found an association between MIR133a and the presence of mutations in all genes studied (p=0.006). A relation between MIR486 and mutations in ZASP and DES (p=0.035) was also noted. Conclusions: • MIR208a seems to have a protective function in the muscle fiber; • Heterogeneity could be related to the concentration of gene expression in each patient; • Expression of myoMIRs influences several aspects in the muscle function through genes modulation which are important to myogenesis control;
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Vázquez Mera, Sara, Pablo Miguéns-Suárez, Laura Martelo-Vidal, Uxío Calvo-Álvarez, Javier Rodríguez-García, Coral González-Ferández, Mar Mosteiro-Añón, et al. "Exosomal miR16, miR21, miR126, miR146a and miR215 as biomarkers for asthma severity." In ERS Lung Science Conference 2022 abstracts. European Respiratory Society, 2022. http://dx.doi.org/10.1183/23120541.lsc-2022.97.
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Wildung, M., C. Herr, M. Alevra, S. Andreas, D. Riedel, and M. Lizé. "miR449 protects airway cilia and healthy lung ageing." In ERS International Congress 2018 abstracts. European Respiratory Society, 2018. http://dx.doi.org/10.1183/13993003.congress-2018.lsc-1038.
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Dong, Jianchun, Amy Asawachaicharn, Stephen J. Tapscott, and Babak A. Parviz. "Electronic Detection of Micro RNA Mir2O6 with Molecularly-Differentiated Nanoelectrodes." In 2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems. IEEE, 2007. http://dx.doi.org/10.1109/nems.2007.352157.
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Bibaki, Eleni, Theodora Georgopoulou, Chara Koutoulaki, Eliza Tsitoura, Eirini Vasarmidi, Nikolaos Tzanakis, and Katerina Antoniou. "The expression of miR185, miR29a and their targets in IPF and lung cancer (LC). A BALF study." In ERS International Congress 2017 abstracts. European Respiratory Society, 2017. http://dx.doi.org/10.1183/1393003.congress-2017.oa480.
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Arya, Deepak, P. Sasikala, Shang Li, Dasaradhi Palakodeti, Cecil Ross, and Sudhir Krishna. "Abstract 1931: MiR182 mediated control over myeloid differentiation provides novel mechanism of Imatinib resistance in chronic myeloid leukemia." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-1931.
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Zhu, Shoumin, Zheng Chen, Dunfa Peng, Tian-ling Hu, Heng Lu, Mohammed Soutto, and Wael El-Rifai. "Abstract 784: Epigenetic silencing of miR490-3p byH. pyloriactivates DARPP-32 and induces resistance to gefitinib in gastric cancer cells." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-784.
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Zhu, Shoumin, Zheng Chen, Dunfa Peng, Tian-ling Hu, Heng Lu, Mohammed Soutto, and Wael El-Rifai. "Abstract 784: Epigenetic silencing of miR490-3p byH. pyloriactivates DARPP-32 and induces resistance to gefitinib in gastric cancer cells." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-784.
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Tian, Zhongxian, Xiaohui Hua, Jiheng Xu, Junlan Zhu, Jingxia li, and Chuanshu Huang. "Abstract 4340: A novel fluoride (ChlA-F) transcriptionally upregulates miR494 via a HuR/JunB axis to inhibit cell invasion in human bladder cancers." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-4340.
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Tian, Zhongxian, Xiaohui Hua, Jiheng Xu, Junlan Zhu, Jingxia li, and Chuanshu Huang. "Abstract 4340: A novel fluoride (ChlA-F) transcriptionally upregulates miR494 via a HuR/JunB axis to inhibit cell invasion in human bladder cancers." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-4340.
Повний текст джерелаЗвіти організацій з теми "MiR486"
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Eshed, Yuval, and Sarah Hake. Exploring General and Specific Regulators of Phase Transitions for Crop Improvement. United States Department of Agriculture, November 2012. http://dx.doi.org/10.32747/2012.7699851.bard.
Повний текст джерелаАнотація:
The transition of plants from a juvenile to adult growth phase entails a wide range of changes in growth habit, physiological competence and composition. Strikingly, most of these changes are coordinated by the expression of a single regulator, micro RNA 156 (miR156) that coordinately regulates a family of SBP genes containing a miR156 recognition site in the coding region or in their 3’ UTR. In the framework of this research, we have taken a broad taxonomic approach to examine the role of miR156 and other genetic regulators in phase change transition and its implication to plant development and crop improvement. We set to: Determine the common and unique factors that are altered upon juvenile to adult phase transition. Determine the functions of select miR156 target genes in tomato and maize, and identify those targets that mediate phase transition. Characterize the role of miR172 and its targets in tomato phase change. Determine the relationships between the various molecular circuits directing phase change. Determine the effects of regulated manipulation of phase change genes on plant architecture and if applicable, productivity. In the course of the study, a new technology for gene expression was introduced – next generation sequencing (NGS). Hence some of the original experiments that were planned with other platforms of RNA profiling, primarily Affymetrix arrays, were substituted with the new technology. Yet, not all were fully completed. Moreover, once the initial stage was completed, each group chose to focus its efforts on specific components of the phase change program. The Israeli group focused on the roles of the DELAYED SYMPODIAL TERMINATION and FALSIFLORA factors in tomato age dependent programs whereas the US group characterized in detail the role of miR156 (also termed Cg) in other grasses and in maize, its interplay with the many genes encoding miR172.
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Moreno, Miguel Ángel. miR96: primer microRNA asociado a una patología monogénica de herencia mendeliana. Sociedad Española de Bioquímica y Biología Molecular (SEBBM), January 2011. http://dx.doi.org/10.18567/sebbmdiv_anc.2011.01.1.
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Whitham, Steven A., Amit Gal-On, and Victor Gaba. Post-transcriptional Regulation of Host Genes Involved with Symptom Expression in Potyviral Infections. United States Department of Agriculture, June 2012. http://dx.doi.org/10.32747/2012.7593391.bard.
Повний текст джерелаАнотація:
Understanding how RNA viruses cause disease symptoms in their hosts is expected to provide information that can be exploited to enhance modern agriculture. The helper component-proteinase (HC-Pro) protein of potyviruses has been implicated in symptom development. Previously, we demonstrated that symptom expression is associated with binding of duplex small-interfering-RNA (duplex-siRNA) to a highly conserved FRNK amino acid motif in the HC-Pro of Zucchini yellow mosaic virus (ZYMV). This binding activity also alters host microRNA (miRNA) profiles. In Turnip mosaic virus (TuMV), which infects the model plant Arabidopsis, mutation of the FRNK motif to FINK was lethal providing further indication of the importance of this motif to HC-Pro function. In this continuation project, our goal was to further investigate how ZYMV and TuMV cause the mis-expression of genes in cucurbits and Arabidopsis, respectively, and to correlate altered gene expression with disease symptoms. Objective 1 was to examine the roles of aromatic and positively charged residues F164RNH and K215RLF adjacent to FR180NK in small RNA binding. Objective 2 was to determine the target genes of the miRNAs which change during HC-Pro expression in infected tissues and transgenic cucumber. Objective 3 was to characterize RNA silencing mechanisms underlying differential expression of host genes. Objective 4 was to analyze the function of miRNA target genes and differentially expressed genes in potyvirus-infected tissues. We found that the charged K/R amino acid residues in the FKNH and KRLF motifs are essential for virus viability. Replacement of K to I in FKNH disrupted duplex-siRNA binding and virus infectivity, while in KRLF mutants duplex-siRNA binding was maintained and virus infectivity was limited: symptomless following a recovery phenomenon. These findings expanded the duplex-siRNA binding activity of HC-Pro to include the adjacent FRNK and FRNH sites. ZYMV causes many squash miRNAs to hyper-accumulate such as miR166, miR390, mir168, and many others. Screening of mir target genes showed that only INCURVATA-4 and PHAVOLUTA were significantly upregulated following ZYMVFRNK infection. Supporting this finding, we found similar developmental symptoms in transgenic Arabidopsis overexpressing P1-HC-Pro of a range of potyviruses to those observed in miR166 mutants. We characterized increased transcription of AGO1 in response to infection with both ZYMV strains. Differences in viral siRNA profiles and accumulation between mild and severe virus infections were characterized by Illumina sequencing, probably due to the differences in HC-Pro binding activity. We determined that the TuMV FINK mutant could accumulate and cause symptoms in dcl2 dcl4 or dcl2 dcl3 dcl4 mutants similar to TuMV FRNK in wild type Arabidopsis plants. These dcl mutant plants are defective in antiviral defenses, and the results show that factors other than HC-ProFRNK motif can induce symptoms in virus-infected plants. As a result of this work, we have a better understanding of the FRNK and FKNH amino acid motifs of HC-Pro and their contributions to the duplex-siRNA binding functions. We have identified plant genes that potentially contribute to infectivity and symptoms of virus infected plants when they are mis-expressed during potyviral infections. The results establish that there are multiple underlying molecular mechanisms that lead viral pathogenicity, some dependent on HC-Pro. The potential benefits include the development of novel strategies for controlling diseases caused by viruses, methods to ensure stable expression of transgenes in genetically improved crops, and improved potyvirus vectors for expression of proteins or peptides in plants.
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Eshed, Yuval, and Sarah Hake. Shaping plant architecture by age dependent programs: implications for food, feed and biofuel. United States Department of Agriculture, December 2012. http://dx.doi.org/10.32747/2012.7597922.bard.
Повний текст джерелаАнотація:
Age dependent programs are responsible for the physiological and developmental differences of young and mature plants. These include a range of morphological characters such as leaf shape and leaf composition (waxes, lignin etc..) but also different in developmental potentials. Apical buds of juvenile plants are vegetative, while those of mature plants can be reproductive. Likewise, basal buds form in the axills of juvenile leaves have different fates than distal buds formed in the axils of mature leaves. The goal of our joint project is to understand and exploit theses age related programs for specific improvement of crop plants. To that end both the WIS group and the PGEC group are using mutants with age related defects as well as modified expression of miR156 to modify age related programs in crop plants- Tomato and potato in Israel and Maize, switchgrass and Brchipodium in the US. In the US, major effort were made to: Characterize the contribution of selected miR156 target genes to yield component traits of maize. Functional analysis of microRNAs and their targets in new crop plants. In Israel, the research progressed in several directions: Understanding the interplay between age dependent programs and the potential of tomato and potato meristems to produce tubers. Evaluation of the agronomic value of mutants that alter flowering regime in side shoots in general, and in the sympodial buds in particular Characterization of wild type axillary buds, comparing shoot ontogeny of gradually maturing apices from basal and distal positions along the main shoot of tomato.
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Lers, Amnon, and Pamela J. Green. Analysis of Small RNAs Associated with Plant Senescence. United States Department of Agriculture, March 2013. http://dx.doi.org/10.32747/2013.7593393.bard.
Повний текст джерелаАнотація:
Senescence is an agriculturally significant process due to its negative impact to crop yield and postharvest quality. The genetic regulatory systems controlling senescence induction and progress respond to both developmental and environmental stress signals and involve numerous gene expression changes. Knowledge about the key molecular factors which control senescence is very limited. MicroRNAs (miRNAs) are a class of small RNAs which typically function by guiding cleavage of target messenger RNAs. They have been shown to play major roles in a variety of plant processes including development, responses to environmental stresses, and senescence. The long-term goal of this work is to elucidate roles of small RNAs associated with plant senescence. The hypothesis underlying this research is that miRNA-mediated regulation makes important contributions to the senescence process in plants. Specific, original research objectives included: 1) Profiling of small RNAs from senescing plants; 2) Data Analysis and public access via a user-friendly web interface; 3) Validation of senescence-associated miRNAs and target RNAs; 4) Development of transgenic plants for functional analysis of miRNAs in Arabidopsis. Major revisions made in the research compared to the original work plan included 1) Exclusion of the planned work with tomato as recommended by the BARD review panel; 2) Performing miRNA study also in senescing Arabidopsis siliques, in addition to senescing leaves. To identify senescenceregulation of miRNAs in Arabidopsis thaliana, eight small RNA libraries were constructed and sequenced at four different stages of development and senescence from both leaves and siliques, resulting in more than 200 million genome-matched sequences. Parallel Analysis of RNA Ends (PARE) libraries, which enable the large-scale examination of miRNA-guided cleavage products, were also constructed and sequenced, resulting in over 750 million genome-matched sequences. These massive datasets lead to the identification of new miRNAs, as well as new regulation of known miRNAs and their target genes during senescence, many of which have established roles in nutrient responsiveness and cell structural integrity. In keeping with remobilization of nutrients thought to occur during senescence, many miRNAs and targets had opposite expression pattern changes between leaf and silique tissues during the progression of senescence. Taken together, these findings highlight the integral role that miRNAs may play in the remobilization of resources and alteration of cellular structure that is known to occur in senescence. Experiments were initiated for functional analysis of specific senescence-associated miRNAs and respective target genes. Transgenic Arabidopsis plants were generated in which miR408, found in this study to be significantly induced in leaf senescence, was over-expressed either constitutively or under a senescence-specific promoter. These plants are currently being characterized for any altered phenotypes. In addition T-DNA knock out mutants for various target genes identified in this research are being analyzed. This work provides insights about specific miRNAs that contribute to leaf and silique senescence. The knowledge generated may suggest new strategies to monitor and alter the progression of senescence in crops for agricultural improvement.