Дисертації з теми "Microscopy tools"
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Mignard-Debise, Lois. "Tools for the paraxial optical design of light field imaging systems." Thesis, Bordeaux, 2018. http://www.theses.fr/2018BORD0009/document.
Повний текст джерелаLight field imaging is often presented as a revolution of standard imaging. Indeed, it does bring more control to the user over the final image as the spatio-angular dimensions of the light field offer the possibility to change the viewpoint and refocus after the shot and compute the scene depth map.However, it complicates the work of the optical designer of the system for two reasons. The first is that there exist a multitude of different light field acquisition devices, each with its own specific design. The second is that there is no model that relates the camera design to its optical properties of acquisition and that would guide the designer in his task. This thesis addresses these observations by proposing a first-order optical model to represent any light field acquisition device. This model abstracts a light field camera as en equivalent array of virtual cameras that exists in object space and that performs the same sampling of the scene. The model is used to study and compare several light field cameras as well as a light field microscope setup which reveals guidelines for the conception of light field optical systems. The simulations of the model are also validated through experimentation with a light field camera and a light field microscope that was constructed in our laboratory
Reeve, James Edward. "Functional dyes as tools for neurophysiology." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:8d8e7fa1-0f1d-4ff5-9f90-6915b15c1ad4.
Повний текст джерелаBola, Sampol Raúl. "Development of optical tools for biological applications based on acousto-optic technology." Doctoral thesis, Universitat de Barcelona, 2021. http://hdl.handle.net/10803/672264.
Повний текст джерелаPer poder garantir l’avanç de les ciències de la vida, amb la gran repercussió a la salut mundial que això comporta, és important anar millorant les eines amb les quals els científics (especialment els biòlegs) desenvolupen els experiments, podent així millorar la quantitat i el volum de les dades extretes. Amb noves eines, els investigadors poden desenvolupar i dissenyar nous experiments que proporcionin informació rellevant sobre l’estudi i la comprensió de molts processos cel·lulars i malalties. La tesi tracta sobre el desenvolupament de dos sistemes òptics amb gran aplicació en el camp de la biologia, ambdues basades en un element modulador de llum comú, els deflectors acusto-òptics (AODs). El AODs són dispositius totalment analògics, on s’utilitzen ones acústiques per poder modular i deflectar un làser amb una gran precisió i velocitat. A la primera part, s’explica el desenvolupament d’un sistema d’atrapament òptic i mesura de força. El sistema permet atrapar i manipular, de manera estable, múltiples objectes, així com realitzar oscil·lacions controlades. Al mateix temps, el sistema és totalment compatible amb mesura de forces per canvis de moment. Tot això permet paral·lelitzar experiments a l’interior cel·lular de manera totalment invasiva, oferint informació sobre les propietats mecàniques de diverses estructures biològiques. A la segona part, es presenta una nova forma d’entendre i utilitzar aquests dispositius: l’holografia acusto-òptica. Mitjançant la generació de senyals acústiques complexes, els AODs permeten projectar patrons de llum arbitraris, més enllà del seu ús principal com a deflectors làser. Això porta al desenvolupament d’un nou microscopi confocal, totalment programable i sense elements mecànics o mòbils. El microscopi permet projectar una infinitat de patrons de llum estructurada, per tal d’obtenir reconstruccions d’alta qualitat a centenars d’imatges per segon. Aquesta nova plataforma de microscòpia d’estat sòlid, permet investigar i implementar una infinitat de maneres d’imatge, per adaptar-se a les necessitats de cada experiment i / o mostra.
Logan, Savannah. "Imaging Vibrio Cholerae Invasion and Developing New Tools for 3D Microscopy of Live Animals." Thesis, University of Oregon, 2019. http://hdl.handle.net/1794/24524.
Повний текст джерелаBaker, Ryan. "IMAGING AND ANALYSIS OF LARVAL ZEBRAFISH GUT MOTILITY, AND AUTOMATED TOOLS FOR 3D MICROSCOPY." Thesis, University of Oregon, 2018. http://hdl.handle.net/1794/23133.
Повний текст джерелаVathalloor, Mathew Manoj. "Neuron guidance and nano-neurosurgery using optical tools." Doctoral thesis, Universitat Politècnica de Catalunya, 2009. http://hdl.handle.net/10803/33075.
Повний текст джерелаJones, Debbie. "Fluorescence spectroscopy and microscopy as tools for monitoring redox transformations of uranium in biological systems." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/fluorescence-spectroscopy-and-microscopy-as-tools-for-monitoring-redox-transformations-of-uranium-in-biological-systems(e5420e94-b96e-4ee1-be63-1a3363672014).html.
Повний текст джерелаRosenthal, Malte [Verfasser]. "Clickmers and Aptamers as versatile tools for drug testing and fluorescence microscopy techniques / Malte Rosenthal." Bonn : Universitäts- und Landesbibliothek Bonn, 2020. http://d-nb.info/1224270592/34.
Повний текст джерелаALMEIDA, FILHO AMERICO de. "Influencia da preparacao previa de amostras de aco AISI H 13 no comportamento a nitretacao." reponame:Repositório Institucional do IPEN, 1999. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10775.
Повний текст джерелаMade available in DSpace on 2014-10-09T14:10:26Z (GMT). No. of bitstreams: 1 06769.pdf: 4275325 bytes, checksum: f2158eb766fa7886249500f40de27cec (MD5)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Dissertacao (Mestrado)
IPEN/D
Instituto de Pesquisas Energeticas e Nucleares, IPEN-CNEN/SP
FAPESP:97/04424-5
Hernández-Neuta, Iván. "Nucleic acid analysis tools : Novel technologies and biomedical applications." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-146334.
Повний текст джерелаAt the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 1: Manuscript.
Kamberger, Robert [Verfasser], and J. [Akademischer Betreuer] Korvink. "Manufacturing Methods for Magnetic Resonance Microscopy Tools with Application to Neuroscience / Robert Kamberger ; Betreuer: J. Korvink." Karlsruhe : KIT-Bibliothek, 2017. http://d-nb.info/1135266255/34.
Повний текст джерелаLópez, Conesa Lluís. "Advanced TEM imaging tools for materials science." Doctoral thesis, Universitat de Barcelona, 2015. http://hdl.handle.net/10803/395195.
Повний текст джерелаLa reducció en l'escala espacial associada a la revolució de la Nanociència i la Nanotecnologia fa necessari comptar amb una sèrie d'eines capaces d'assolir una resolució sense precedents en una gran varietat d'àress, ja no tan sols com a control de qualitat, sinó per tal d'entendre les propietats de la matèria a la nanoescala. La correlació de la configuració estructural, la composició química i les distribucions de càrrega amb les propietats funcionals és imprescindible pel disseny de nous dispositius, tant des de la perspectiva 'top down' (reducció de les dimensions dels dispositius) com de la perspectiva 'bottom up' (fabricació d'estructures complexes a partir de blocs més petits, fins i tot àtoms). La capacitat de la Microscòpia Electrònica de Transmissió (TEM) de proporcionar diferents tipus d'informació amb una alta resolució espacial, situa les tècniques avançades de TEM com a peça clau en el desenvolupament d'aquest camp multidisciplinari i creixent. L'objectiu principal d'aquesta tesi ha estat l'aplicació de tècniques quantitatives d'imatge TEM per la resolució de problemes en ciència dels materials. La tesi cobreix un espectre ampli pel que fa al tipus de materials estudiats i els seus camps d'aplicació. El Capítol 1 presenta una introducció general a la teoria de formació d'imatge aplicada a la microscopia TEM. S'hi exposen els diferents fenòmens d'interacció electró-matèria que són responsables dels diferents tipus de contrast que es poden trobar a les imatges TEM. El Capítol 2 presenta les tècniques experimentals que es faran servir en la caracterització dels materials, en concret la simulació d'imatges d'alta resolució (HRTEM), l'holografia electrònica i l'anàlisi de la fase geomètrica (GPA). S'hi pot trobar una descripció del marc teòric i dels fonaments experimentals, juntament amb un resum dels resultats més recents en aquests camps. Els resultats experimentals s'agrupen en els capítols posteriors segons la dimensionalitat dels sistemes estudiats. En ordre decreixent de dimensionalitat s'hi inclouen: materials massius (3D), capes primes (2D), nanofils (1D) i nanopartícules (1D).
Diederich, Benedict [Verfasser], Rainer [Gutachter] Heintzmann, and Christian [Gutachter] Eggeling. "Democratizing microscopy by introducing innovative Open-Source hard and software tools / Benedict Diederich ; Gutachter: Rainer Heintzmann, Christian Eggeling." Jena : Friedrich-Schiller-Universität Jena, 2021. http://d-nb.info/1239177542/34.
Повний текст джерелаWoringer, Maxime. "Tools to analyze single-particle tracking data in mammalian cells." Electronic Thesis or Diss., Sorbonne université, 2019. http://www.theses.fr/2019SORUS419.
Повний текст джерелаThis work aims at providing tools to dissect the regulation of transcription in eukaryotic cells, with a focus on single-particle tracking of transcription factors in mammalian cells. The nucleus of an eukeryotic cell is an extremely complex medium, that contains a high concentration of macromolecules (DNA, RNA, proteins) and other small molecules (ATP, etc). How these molecules interact with transcription factors, and thus influence transcription rates is an area of intense investigations. Although some of these interactions can be captured by regular biochemistry, many of them, including weak, non-covalent interactions remain undetected by these methods. Live-cell imaging and single-particle tracking (SPT) techniques are increasingly used to characterize such effects. The inference of biophysical parameters of a given transcription factor (TF), such as its diffusion constant, the number of subpopulations or its residence time on DNA, are crucial to understanding how TF dynamics and transcription intertwine. Accurate and validated SPT analysis tools are needed. To be used by the community, SPT tools should not only be carefully validated, but also be easily accessible to non-programmers. They should also be designed to take into account known biases of the imaging techniques. In this work, we first propose a tool, accessible through a web interface, based on the modeling of the diffusion propagator. We validate it extensively and show that it exhibits state-of-the art performance. We apply this tool to two experimental settings: (1) the study of catalysis-enhanced diffusion in-vitro and (2) the analysis of the dynamics of the c-Myc transcription factor in mammalian cells
Ebai, Tonge. "Development of Enhanced Molecular Diagnostic Tools for Protein Detection and Analysis." Doctoral thesis, Uppsala universitet, Molekylära verktyg, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-320380.
Повний текст джерелаXu, Daxue. "Analyses of Particulate Contaminants in Semiconductor Processing Fluids." Thesis, University of North Texas, 1998. https://digital.library.unt.edu/ark:/67531/metadc500968/.
Повний текст джерелаWatabe, Tetsuya. "Booster, a Red-Shifted Genetically Encoded Förster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263527.
Повний текст джерелаGrah, Joana Sarah. "Mathematical imaging tools in cancer research : from mitosis analysis to sparse regularisation." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/273243.
Повний текст джерелаSchulte, Lukas [Verfasser], Holger [Akademischer Betreuer] Stark, Holger [Gutachter] Stark, and Ralf [Gutachter] Ficner. "New Computational Tools for Sample Purification and Early-Stage Data Processing in High-Resolution Cryo-Electron Microscopy / Lukas Schulte ; Gutachter: Holger Stark, Ralf Ficner ; Betreuer: Holger Stark." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2019. http://d-nb.info/1175204889/34.
Повний текст джерелаLuther, Ilse. "Semen characteristics of free-ranging African elephants (Loxodonta africana) and Southern white rhinoceros (Ceratotherium simum simum) using Computer-aided sperm analysis, Electron microscopy and Genomics as diagnostic tools." University of the Western Cape, 2016. http://hdl.handle.net/11394/5443.
Повний текст джерелаThe survival of free-ranging (in situ) African elephant and Southern white rhinoceros populations are currently being challenged on a daily basis in Africa. Reproductive health is considered a vital component of species conservation. Conservation of the last mega land mammals may ultimately require intervention by breeding management or combined with assisted reproductive technologies (ART). There is a strong case for gathering baseline information, both physiological and biological, of any species, as opportunities arise. During this study a total number of 21 ejaculates collected over two seasons from 12 free-ranging African elephant bulls were characterised, as well as 10 ejaculates collected from 10 free-ranging Southern white rhinoceros bulls from two populations. Ejaculates were collected from adult bulls by means of electroejaculation under anaesthesia. Routine semen analysis was combined with Computer-aided sperm analysis (CASA), Computer-aided sperm morphology analysis (CASMA), Transmission electron microscopy (TEM) and Genomics as diagnostic tools. Additionally, sperm functionality within different media was investigated and sperm subpopulation classification according to the motion pattern displayed. The results presented is based on the evaluation and classification of ≈ 45 000 individual African elephant spermatozoa and ≈ 18 000 individual Southern white rhinoceros spermatozoa. The average elephant ejaculate contained a total number of 47 x 10⁹ spermatozoa (volume of 56 ± 38mL x concentration of 818 ± 750 x 10⁶/mL) that recorded a total motility of 81 ± 29% of which 62 ± 26% were progressively motile. CASA recorded velocities for curvilinear velocity (VCL 241 ± 58μm/s), straight-line velocity (VSL 173 ± 181μm/s) and average path velocity (VAP 201 ± 54μm/s), and kinematics at straightness of track (STR 86 ± 85%), linearity of track (LIN 67 ± 16%), amplitude of lateral head displacement (ALH 4 ± 0.75μm) and beat cross frequency (BCF 21 ± 3Hz). Structural analysis revealed 68 ± 11% of the spermatozoa were viable (intact plasma membrane) and 77 ± 11% maintained acrosome integrity. Ejaculates contained 55 ± 14% morphologically normal spermatozoa, CASMA measured sperm head lengths at 6.83 ± 0.26μm and width 3.32 ± 0.18μm (total head area of 20.17 ± 1.96μm²) of which 38.95 ± 0.92% is covered by an acrosomal cap. The average rhinoceros ejaculate contained a total number of 1.1 x 10⁹ spermatozoa (volume of 24 ± 24mL x concentration of 83 ± 96 x 10⁶/mL) that recorded a total motility at 82 ± 8% of which 28 ± 23% were progressively motile. CASA recorded velocities for VCL (85 ± 29μm/s), VSL (44 ± 25μm/s) and VAP (69 ± 30μm/s, and kinematics at STR (63 ± 14%), LIN (51 ± 16%), ALH (2 ± 0.16μm) and BCF (16 ± 6Hz). Structural analysis revealed 73 ± 10% of the spermatozoa were viable (intact plasma membrane) and 76 ± 4% maintained acrosome integrity. Ejaculates contained 62 ± 14% morphologically normal spermatozoa, CASMA measured sperm head lengths at 5.5 ± 0.17μm and width 2.9 ± 0.19μm (total head area of 14.8 ± 1.43μm²) of which 36.3 ± 0.59% is covered by an acrosomal cap. Based on a Boolean argument and CASA data exploration it was possible to derive elephant and rhinoceros CASA cut-off criteria to sort between activated and hyperactivated motile spermatozoa. For the genomic component of this study, the CatSper1 (Loxodonta africana) gene was identified,sequenced and verified in a free-ranging (natural) African elephant population. Multivariate analysis(MVA) was applied to examine the associations between the semen and sperm parameters and the traits they accounted for in this study. Our understanding of wildlife reproductive sciences can substantially progress as the analytical techniques applied and the combination thereof is expanded. This investigation presents a new set of comprehensive semen and sperm threshold values for future investigations.
Alaoui, Lasmaili Karima El. "Caractérisation au moyen d'outils mathématiques des effets vasculaires du bevacizumab à des fins d'optimisation des protocoles thérapeutiques dans le cas des tumeurs cérébrales." Thesis, Université de Lorraine, 2017. http://www.theses.fr/2017LORR0023/document.
Повний текст джерелаThe main aim of this work was to characterize the effects of the anti-VEGF Bevacizumab (Avastin) on the tumor vascular network, in vivo, over time, thanks to the skin fold chamber model on the nude mouse. Images of the vascular network obtained using intravital microscopy were analyzed par a dedicated image processing algorithm developed within our research team, allowing to highlight the morphological modifications induced by the treatment and to isolate discriminating parameters of the vascular "normalization", by comparison to healthy vascular networks. Le vascular "normalization" period detected with our tool was comforted by the analysis of the functionality of the blood vessels over time, in vivo and by an immunohistochemical analysis of the blood vessels and of the tumor tissue. In preliminary in vivo experiments, we tried to verify the hypothesis of the benefits of an anti-VEGF treatment prior to photodynamic therapy (PDT) on glioblastoma xenografts implanted subcutaneously or in the skin fold chamber. The efficacy of PDT is described as being dependent on tumor oxygenation and on the distribution of the photosensitizing agent within the tumor. In paralel to this work, we tried as a pluridisciplinary team to develop a mathematical model of the tumor response to bevacizumab using biological data obtained on the same in vivo model et that will allow in the future to simulate the response for different doses and different treatment durations, for the optimization of therapeutic protocols
Raman, Purnima. "Freeze drying microscopy as a tool to study sublimation kinetics." Thesis, Loughborough University, 2015. https://dspace.lboro.ac.uk/2134/18316.
Повний текст джерелаAljamal, Mohammad Abdulraheem. "Comparison of Microscopic and Mesoscopic Traffic Modeling Tools for Evacuation Analysis." Thesis, Virginia Tech, 2017. http://hdl.handle.net/10919/79592.
Повний текст джерелаMaster of Science
Wan, Yong. "Fluorender, an interactive tool for confocal microscopy data visualization and analysis." Thesis, The University of Utah, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=3592436.
Повний текст джерелаConfocal microscopy has become a popular imaging technique in biology research in recent years. It is often used to study three-dimensional (3D) structures of biological samples. Confocal data are commonly multichannel, with each channel resulting from a different fluorescent staining. This technique also results in finely detailed structures in 3D, such as neuron fibers. Despite the plethora of volume rendering techniques that have been available for many years, there is a demand from biologists for a flexible tool that allows interactive visualization and analysis of multichannel confocal data. Together with biologists, we have designed and developed FluoRender. It incorporates volume rendering techniques such as a two-dimensional (2D) transfer function and multichannel intermixing. Rendering results can be enhanced through tone-mappings and overlays. To facilitate analyses of confocal data, FluoRender provides interactive operations for extracting complex structures. Furthermore, we developed the Synthetic Brainbow technique, which takes advantage of the asynchronous behavior in Graphics Processing Unit (GPU) framebuffer loops and generates random colorizations for different structures in single-channel confocal data. The results from our Synthetic Brainbows, when applied to a sequence of developing cells, can then be used for tracking the movements of these cells. Finally, we present an application of FluoRender in the workflow of constructing anatomical atlases.
Wong, Tsz-wai Terence, and 黃子維. "Optical time-stretch microscopy: a new tool for ultrafast and high-throughput cell imaging." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hub.hku.hk/bib/B5066234X.
Повний текст джерелаpublished_or_final_version
Electrical and Electronic Engineering
Master
Master of Philosophy
Allwell-Brown, Gbemisola. "Individual and household-level determinants of malaria infection in under-5 children from north-west and southern Nigeria : A cross-sectional comparative study based on the 2015 Nigeria Malaria Indicator Survey." Thesis, Uppsala universitet, Internationell mödra- och barnhälsovård (IMCH), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-324360.
Повний текст джерелаNyflött, Åsa. "Development of an Image Processing Tool for Fluorescence Microscopy Analysis of Paper Chemistry." Thesis, Karlstads universitet, Fakulteten för teknik- och naturvetenskap, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-6990.
Повний текст джерелаTillverkning av papper är till en viss del baserad på empirisk kunskap. Välkänt är att finmaterial, pH värde, laddning och jonstyrka påverkar de papperskemiska mekanismerna och därmed flertalet pappersegenskaper vid tillverkning av papper. En möjlighet att utveckla kunskaperna inom papperskemiska mekanismerar att studera finmaterial och additiv i en pappers suspension for att samla in informationom reaktionsmekanismer. Fyra trackningalgoritmer ar vidareutvecklade i syftet att möjliggöra studier kring papperskemiska mekanismer. Trackningalgoritmerna inkluderar två varianter av den välkända "Lucas-Kanade" algoritm och två template-baserade metoder: korskorrelation och minsta kvadratmetoden. Samtliga metoder bygger på samma princip, men trots detta kan resultaten från trackningen skilja mellan metoderna. Lucas-Kanade algoritmerna är mer oberoende av brus medan korskorrelationen och minsta kvadratmetoden exekveras snabbare i Matlab. Trackning metoderna utvärderades med hjälp av en simulator som genererar bildsekvenser av syntetiska partiklar med en Brownsk rörelse. Trackningen har även använts på mikroskopibilder av rörelsebanor på verkliga suspenderade latex partiklar, varvid trackningresultatet har jämförts med manuell trackning. De genererade bildsekvensernapa de simulerade partiklarna har kända rörelsebanor som är jämförbara med rörelsebanor for latex partiklarna.
Tavakoli, Mitra. "Corneal confocal microscopy : a novel non-invasive diagnostic tool for assessing peripheral neuropathies." Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.492280.
Повний текст джерелаJaouen, Kévin. "Backside absorbing layer microscopy : a new tool for the investigation of 2D materials." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS296/document.
Повний текст джерелаOptical microscopy based on anti-reflective coatings is a simple yet powerful characterization tool which notably allowed the first observation of graphene in 2004. Since then, the field of two-dimensional (2D) materials has developed rapidly both at the fundamental and applied levels. These ultrathin materials present inhomogeneities (edges, grain boundaries, multilayers, etc.) which strongly impact their physical and chemical properties. Thus their local characterization is essential. This thesis focuses on a recent enhanced-contrast optical microscopy technique, named BALM, based on ultrathin (2-5 nm) and strongly light-absorbing (metallic) anti-reflective layers. The goal is notably to evaluate the benefits of this technique for the study of 2D materials and their chemical reactivity. The various levers to improve 2D materials observation were investigated and optimized for two model materials: graphene oxide and MoS₂ monolayers. The investigation of molecular layer deposition dynamic notably showed the extreme sensitivity of BALM for such measurements and the significant contribution of multilayers anti-reflective coatings to enhance contrast during the observation of 2D materials. One of the main assets of BALM comes from its combination to other techniques. We particularly considered the coupling between optical measurements and electrochemistry for which the anti-reflective layer serves as working electrode. We investigated optically the dynamic of electrochemical reduction of Graphene Oxide (GO), the electrografting of organic layers by diazonium salts reduction on GO and its reduced form (rGO), as well as the intercalation of metallic ions within GO sheets. By combining versatility and high-contrast, BALM is established as a promising tool for the study of 2D materials, especially for the local and in situ characterization of their chemical and electrochemical reactivity
Gonçalves, Bruno Filipe Pimparel. "Digital imaging processing tools for neuronal images." Master's thesis, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/10584.
Повний текст джерелаOs neurónios são celulas especializadas do Sistema Nervoso, cujas funções se baseiam na correta formação de três compartimentos subcelulares primários – corpo celular, axónio e dendrites – e na rede neuronal que formam para passar a informação entre si. A análise quantitativa das características destas estruturas pode ser usada para estudar a relação entre a morfologia e função neuronal, e monitorizar alterações que ocorram em células individuais ou ao nível da rede, que se possam correlacionar com doenças neurológicas. Nesta tese foi efetuada uma pesquisa de ferramentas digitais disponíveis dedicadas ao processamento e análise de imagens neuronais, com enfoque na sua aplicabilidade para analisar as nossas bioimagens neuronais de fluorescência adquiridas no dia-a-dia. Nos programas selecionados (NeuronJ, NeurphologyJ e NeuriteQuant) foi primeiro avaliada a necessidade de preprocessamento, e os programas foram subsequentemente utilizados em conjuntos de imagens de culturas primárias de córtex de rato para comparar a sua eficácia no processamento destas bioimagens. Os dados obtidos com os vários programas foram comparados com a análise manual usando o ImageJ como ferramenta de análise. Os resultados demonstraram que o programa que aparenta funcionar melhor com as nossas imagens de fluorescência é o NeuriteQuant, porque é automático e dá resultados globalmente semelhantes aos da análise manual, especialmente na avaliação do Comprimento das Neurites por célula. Uma das desvantagens é que a quantificação da ramificação das neurites não dá resultados satisfatórios e deve continuar a ser realizada manualmente. Também realizamos uma pesquisa de ferramentas de processamento de imagem dedicada a imagens de contraste de fase, mas poucos programas foram encontrados. Estas imagens são mais fáceis de obter e mais acessíveis economicamente, contudo são mais difíceis de analisar devido às suas características intrínsecas. Para contornar esta lacuna, estabeleceu-se e otimizou-se uma sequência de processamento e análise para melhor extrair informação neuronal relevante de imagens de contraste de fase utilizando o programa ImageJ. A sequência desenvolvida, na forma de uma macro do ImageJ designada NeuroNet, foi aplicada a imagens de contraste de fase de culturas neuronais em diferentes dias de diferenciação, na presença ou ausência de um inibidor farmacológico, com o objetivo de responder a uma questão científica. A macro NeuroNet desenvolvida provou ser útil para analisar estas bioimagens, existindo contudo espaço para ser aperfeiçoada.
Neurons are specialized cells of the Nervous System, with their function being based on the formation of the three primary sub cellular compartments – soma, axons, and dendrites – and on the neuritic network they form to contact and pass information to each other. The quantitative analysis of the characteristics of these structures can be used to study the relation between neuronal morphology and function, and to monitor distortions occurring in individual cells or at the network level that may correlate with neurological diseases. In this thesis a survey of freely available digital tools dedicated to neuronal images processing and analysis was made with an interest in their applicability to analyse our routinely acquired neuronal fluorescent bioimages. The selected program´ (NeuronJ, NeurphologyJ and NeuriteQuant) preprocessing requirements were first evaluated, and the programs were subsequently applied to a set of images of rat cortical neuronal primary cultures in order to compare their effectiveness in bioimage processing. Data obtained with the various programs was compared to the manual analysis of the images using the ImageJ analysis tool. The result show that the program that seems to work better with our fluorescence images is NeuriteQuant, since it is automatic and gives overall results more similar to the manual analysis. This is particularly true for the evaluation of the Neurite Length per Cell. One of the drawbacks is that the quantification of neuritic ramification does not give satisfactory results and is better to be performed manually. We also performed a survey of digital image processing tools dedicated to phase contrast microphotographs, but very few programs were found. These images are easier to obtain and more affordable in economic terms, however they are harder to analyse due to their intrinsic characteristics. To surpass this gap we have established and optimized a sequence of steps to better extract relevant information of neuronal phase contrast images using ImageJ. The work-flow developed, in the form of an ImageJ macro named NeuroNet, was then used to answer a scientific question by applying it to phase contrast images of neuronal cultures at different differentiating days, in the presence or absence of a pharmacological inhibitor. The developed macro NeuroNet proved to be useful to analyse the images however there is still space to improvement.
Turner, Ian James. "AFM investigations of critical interactions in the Bacillus primosome and Cryogenic AFM : a new tool for structural biology." Thesis, University of Nottingham, 2006. http://eprints.nottingham.ac.uk/10188/.
Повний текст джерелаMaggiano, Corey. "CONFOCAL LASER SCANNING MICROSCOPY AS A TOOL FOR THE INVESTIGATION OF TETRACYCLINE FLUORESCENCE IN ARCHAEOLOGICALHUMAN BONE." Master's thesis, University of Central Florida, 2005. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/2752.
Повний текст джерелаM.S.
Department of Biology
Arts and Sciences
Biology
Vieker, Henning [Verfasser]. "Helium Ion Microscopy: a new tool to analyze and modify nanoscale objects / Henning Vieker." Bielefeld : Universitätsbibliothek Bielefeld, 2014. http://d-nb.info/1064382134/34.
Повний текст джерелаHermann, Martin, Oliver Nussbaumer, Ralf Knöfler, Paul Hengster, Walter Nussbaumer, and Werner Streif. "Real-Time Live Confocal Fluorescence Microscopy as a New Tool for Assessing Platelet Vitality." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-136619.
Повний текст джерелаHintergrund: Die Vitalitätsbestimmung von Blutplättchen ist sowohl für die Analyse angeborener Plättchendefekte als auch für die Qualitätsbestimmung von Plättchenkonzentraten von zentraler Bedeutung. Methoden: In der vorliegenden Arbeit stellen wir eine Methode vor, die mittels einer Kombination von Vitalfarbstoffen und konfokaler «Real time»-Mikroskopie neue Einblicke in die Vitalitätsbestimmung lebender Plättchen ermöglicht. Mittels der Zugabe von FITC-gekoppeltem Weizenkeimlektin (WGA), Tetramethylrhodamin-Methylesterperchlorat (TMRM) und Acetoxymethylester (Rhod-2) wurde bei lebenden Blutplättchen deren Morphologie, mitochondriale Aktivität und Veränderungen im Calcium-Haushalt im Rahmen der Lagerung analysiert. Für die Mikroskopie wurde ein Nipkow-System gewählt, das eine konfokale Mikroskopie lebender Zellen ermöglicht. Ergebnisse: Der Vergleich von 10 humanen Blutplättchenproben zu Beginn bzw. nach 5 und 7 Tagen Lagerung zeigte einen Anstieg der Rhod-2-positiven Plättchen von 3,6 über 47 auf 71%. Die Anzahl der Blutplättchen mit TMRM-positiven Mitochondrien hingegen lag vor der Lagerung bei 95,4% und nach den 7 Tagen Lagerung bei 92,5%. Schlussfolgerung: Die hier vorgestellte Methodik der Bildgebung zur Bestimmung vitaler Parameter von Blutplättchen eignet sich als ergänzende Analysemodalität für eine bessere Bestimmung der Blutplättchenqualität
Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich
Hermann, Martin, Oliver Nussbaumer, Ralf Knöfler, Paul Hengster, Walter Nussbaumer, and Werner Streif. "Real-Time Live Confocal Fluorescence Microscopy as a New Tool for Assessing Platelet Vitality." Karger, 2010. https://tud.qucosa.de/id/qucosa%3A26667.
Повний текст джерелаHintergrund: Die Vitalitätsbestimmung von Blutplättchen ist sowohl für die Analyse angeborener Plättchendefekte als auch für die Qualitätsbestimmung von Plättchenkonzentraten von zentraler Bedeutung. Methoden: In der vorliegenden Arbeit stellen wir eine Methode vor, die mittels einer Kombination von Vitalfarbstoffen und konfokaler «Real time»-Mikroskopie neue Einblicke in die Vitalitätsbestimmung lebender Plättchen ermöglicht. Mittels der Zugabe von FITC-gekoppeltem Weizenkeimlektin (WGA), Tetramethylrhodamin-Methylesterperchlorat (TMRM) und Acetoxymethylester (Rhod-2) wurde bei lebenden Blutplättchen deren Morphologie, mitochondriale Aktivität und Veränderungen im Calcium-Haushalt im Rahmen der Lagerung analysiert. Für die Mikroskopie wurde ein Nipkow-System gewählt, das eine konfokale Mikroskopie lebender Zellen ermöglicht. Ergebnisse: Der Vergleich von 10 humanen Blutplättchenproben zu Beginn bzw. nach 5 und 7 Tagen Lagerung zeigte einen Anstieg der Rhod-2-positiven Plättchen von 3,6 über 47 auf 71%. Die Anzahl der Blutplättchen mit TMRM-positiven Mitochondrien hingegen lag vor der Lagerung bei 95,4% und nach den 7 Tagen Lagerung bei 92,5%. Schlussfolgerung: Die hier vorgestellte Methodik der Bildgebung zur Bestimmung vitaler Parameter von Blutplättchen eignet sich als ergänzende Analysemodalität für eine bessere Bestimmung der Blutplättchenqualität.
Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
Bergmann, Stephan [Verfasser]. "Optimizing single molecule localization microscopy as a tool in chemistry, biology, and physics / Stephan Bergmann." Bielefeld : Universitätsbibliothek Bielefeld, 2020. http://d-nb.info/1204561869/34.
Повний текст джерелаChristou, Nina-Eleni. "Development of NMR as a tool for the structural and dynamic high-resolution characterization of phototranformable fluorescent proteins." Thesis, Université Grenoble Alpes, 2020. http://www.theses.fr/2020GRALY051.
Повний текст джерелаThe discovery of Phototransformable Fluorescent proteins (PTFPs) over the last decades has revolutionized the field of microscopy. Reversibly photo-switchable fluorescent proteins (RSFPs), in particular, are currently routinely used for Super Resolution Microscopy techniques, such as RESOLFT (REversible Saturable OpticaL Fluorescence Transitions). Photo-induced switching between a fluorescent "on"- and a dark "off"-state, in combination with advanced illumination schemes has allowed for imaging nanometer sized compartments in biological cells. Crystallographic studies of such RSFPs have provided useful mechanistic explanations for their photophysical behaviour and has guided fluorescent protein engineering into designing better tags. However, the crystal forms of such proteins studied at cryogenic temperatures fail to capture dynamics present in RSFPs which could potentially play a significant role in their photophysics. So far, only a single NMR study for the RSFP Dronpa has been reported in the literature (Mizuno, 2008). During my PhD thesis, I was able to complement crystallographic studies of rsFolder, a green RSFP, with a dynamic perspective using multidimensional solution NMR spectroscopy.Using a portable in-situ laser illumination device coupled with the NMR spectrometer, I was able to extract quantitative local dynamic information for both the fluorescent "on"- and "off"-states of rsFolder, characterized by a primarily cis and trans chromophore, respectively. NMR signatures of residues in the non-fluorescent "off"-state were identified using LASER-driven Exchange NMR experiments. The metastable photo-induced "off"-state of rsFolder appears more dynamic on the millisecond timescale than the fluorescent "on"-state. NMR investigations of the chromophore resulted in the deciphering of four configurations, populated in a pH-dependent fashion. Moreover, pH-induced cis-trans isomerization of the chromophore was observed, in the absence of light. NMR-derived values of activation energies for isomerization and free energy differences between the cis and trans chromophore enabled the mapping of the ground-state free energy landscape of rsFolder at different pH values and buffer compositions. Lastly, comparing NMR observables with optical measurements on rsFolder and mutants highlights the potential role that NMR can play in the field of RSFP engineering. Altogether, my PhD work yielded in not only a reliable in-situ illumination set-up accompanied with relevant NMR experiments to study RSFPs, but also highlighted the importance of dynamics in understanding RSFPs' photophysical properties
Wendland, Kristin [Verfasser]. "Semi-automated fluorescence microscopy and automated image analysis as a tool to study neuronal survival / Kristin Wendland." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2021. http://d-nb.info/124154090X/34.
Повний текст джерелаFries, Ryan. "Evaluating the impacts of accelerated incident clearance tools and strategies by harnessing the power of microscopic traffic simulation." Connect to this title online, 2007. http://etd.lib.clemson.edu/documents/1181669446/.
Повний текст джерелаGOUVEA, CRISTOL DE PAIVA. "DUAL BEAM MICROSCOPY AS A MODIFICATION AND CHARACTERIZATION TOOL OF ORGANIC SEMICONDUCTOR THIN FILMS AND FOR DEVICE FABRICATION." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2016. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=29615@1.
Повний текст джерелаCOORDENAÇÃO DE APERFEIÇOAMENTO DO PESSOAL DE ENSINO SUPERIOR
PROGRAMA DE SUPORTE À PÓS-GRADUAÇÃO DE INSTS. DE ENSINO
PROGRAMA DE EXCELENCIA ACADEMICA
Nesta tese de doutoramento apresentamos a técnica de microscopia de feixe duplo (MEV e FIB) como uma ferramenta modificadora das propriedades físico-química dos semicondutores orgânicos, a qual pode ser eficaz para alterar e controlar a mobilidade dos portadores de carga nestes materiais semicondutores. Neste caso, filmes finos e dispositivos orgânicos, principalmente à base de tiofeno, foram bombardeados com diferentes doses de íons de Ga com objetivo de induzir modificações na estrutura polimérica a partir das diversas interações entre o íon e o polímero. As propriedades dos filmes finos e dos dispositivos bombardeados foram caracterizadas através das técnicas de UV-Vis, Espectroscopia Raman e CELIV, as quais indicaram a existência de dois regimes de comportamentos governados pela dose de íons empregada. Técnicas avançadas de microscopia eletrônica indicaram a formação de uma estrutura tipo grafítica, em torno de 50 nm da superfície do bombardeamento, decorrente da interação entre os íons de gálio e a camada polimérica. A possibilidade de construir dispositivos orgânicos intercalados com camadas grafíticas pode ser explorada de forma a construir arquiteturas mais eficientes, explorando a alta resolução espacial que a técnica FIB proporciona.
In this doctoral thesis we presented the dual-beam microscopy (SEM and FIB) technique as a modifier tool of physicochemical properties of the organic semiconductors, which it can be effective to change and control the charge carrier mobility into these semiconductor materials. In this case, organic devices and thin films, especially at thiophene base, were bombarded with different Ga ion doses in order to induce modification in the polymeric structure from the various interactions between the ion and the polymer. The bombarded thin films and devices properties were characterized by UV-Vis, Raman spectroscopy and CELIV techniques, which indicated the existence of two behavior regimes governed by the ion dose employed. Advanced electron microscopy techniques indicated the formation of a graphitic structure, around 50 nm from the surface bombardment, resulting of the interaction between the gallium ions and the polymer layer. The possibility to fabricate organic devices interspersed with graphitic layers can be exploited in order to construct more efficient architectures, using the high spatial resolution of the FIB technique.
Axelsson, Eva, and Therese Wilson. "Microscopic simulation as an evaluation tool for the road safety of vulnerable road users." Thesis, Linköpings universitet, Kommunikations- och transportsystem, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-130010.
Повний текст джерелаKilani, Suha School of Medicine UNSW. "The use of polarised light microscopy as a non-invasive tool for early assessment of human oocytes and embryos." Awarded by:University of New South Wales. School of Medicine, 2007. http://handle.unsw.edu.au/1959.4/35247.
Повний текст джерелаThevathasan, Jervis Vermal [Verfasser], and Jonas [Akademischer Betreuer] Ries. "Establishing single-molecule localization microscopy as a quantitative tool towards structural cell biology. / Jervis Vermal Thevathasan ; Betreuer: Jonas Ries." Heidelberg : Universitätsbibliothek Heidelberg, 2020. http://d-nb.info/122083629X/34.
Повний текст джерелаHolroyd, Dale. "Atomic force microscopy : a novel tool for the analysis of the mechanism of action of antimicrobial peptides on target membranes." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53306.
Повний текст джерелаENGLISH ABSTRACT: Nanoscale visualisation of live cells and cellular components under physiological conditions has long been a goal in microscopy. The objective of this study was to validate the use of Atomic Force Microscopy (AFM) as a new tool in unravelling the mysteries of antimicrobial peptide mechanism of action. Using the simplest AFM imaging technique, we were able to analyse the influence of haemolytic melittin and anti-bacterial magainin 2 on different target membranes at nanometer resolution, without using fixing agents. First, magainin 2 was synthesised and purified by gel permeation chromatography and high performance liquid chromatography (HPLC). The purity of magainin 2 and melittin, isolated from bee venom (Sigma-Aldrich), was verified with electro spray ionisation mass spectrometry (ESI-MS). Second, dose-response experiments were used to determine the optimum peptide/target cell ratio that would allow interaction with the membrane without causing lysis. Third, peptide/target-cell samples were placed on silica plates and visualised using contact mode AFM. Images obtained of the cells before and after peptide treatment, showed distinct changes in cell membrane surface topology. We observed grooves, lesions, membrane collapse and vesiculation depending on the concentration, type of peptide and target-cell used, allowing us to make conclusions regarding the mechanism of action of melittin and magainin 2. In comparison with model membrane studies, our AFM results show that a peptide can function by more than one mechanism of action depending on the structural composition of the membrane, which appears to have specific segregated lateral organisation. Magainin 2 (non-toxic) selectively targets cell membranes using different mechanisms of action. In this way it can lyse bacterial membranes (anti-bacterial agent) using one mechanism, while using another mechanism to interact with mammalian cells at physiological concentrations, without destroying them. In contrast, melittin (toxic) is non-selective, and uses the same mechanism of interaction with bacterial and mammalian cells. In conclusion, we propose a new holistic model for the mechanism of action of antimicrobial peptides.
AFRIKAANSE OPSOMMING: Nanoskaal visualiseering van lewende selle en sellulêre komponente onder fisiologiese toestande is al 'n geruime tyd 'n mikpunt in mikroskopie. Die doel van hierdie studie was om antimikrobiese peptiede se meganisme van werking op teikenselle op nanoskaalvlak met AFM te visualiseer. Sonder om fikseermiddels by te voeg, het ons die eenvoudigste AFM tegniek gebruik om die effek van hemolitiese melittien en anti-bakteriële magainin 2 op verskillende teikenselle, in nanometer resolusie, waar te neem. Eerstens is Magainin 2 gesintesiseer en gesuiwer met behulp van gelpermeasie chromatografie en hoë doeltreffenheid vloeistof chromatografie (HPLC). Die suiwerheid van magainin 2 en kommersiële bye gif melittien, is bevestig met behulp van elektrosproei-ionisasie massaspektrometrie (ESI-MS). Tweedens, is dosis-respons eksperimente gebruik om die optimale peptied/teikensel verhouding te bepaal voordat membraanliese plaasvind. Derdens, is peptied/teikensel monsters op silika plate gevisualiseer met gebruik van kontak AFM. Die beelde van die selle, voor en na peptied behandeling, het duidelike veranderinge in seltopologie getoon. Ons het groewe, letsels, membraaninstorting en vesikulasie, afhangende van die konsentrasie peptied en teikensel gebruik, waargeneem. Dit het ons toegelaat om tot gevolgtrekkings te kom aangaande die meganisme van werking van melittien en magainin 2. In ooreenstemming met model membraan studies, het ons AFM resultate gewys dat 'n peptied veelvoudige meganismes van werking kan hê, afhangend van die strukturele samestelling van die membraan, wat klaarblyklik laterale segregasie toon. Magainin 2 (nie-giftig) is selektief ten opsigte van teikenselle omdat dit gebruik maak van verskillende meganismes van werking op bakteriële en soogdier selle. In teenstelling is melittien (giftig) nie-selektief, en gebruik dieselfde meganisme van werking op bakteriële en soogdierselle. Ten slotte, stel ons 'n nuwe model vir die meganisme van werking voor.
Amante, Joseph David. "Scanning Methods as Monitoring, Verification, and Accounting tools for CO₂ Sequestration in Unconventional Gas Reservoirs." Thesis, Virginia Tech, 2015. http://hdl.handle.net/10919/76047.
Повний текст джерелаMaster of Science
Khadhraoui, Boutheina. "éco-extraction assistée par ultrasons des plantes médicinales : mécanisme(s), intensification et industrialisation ULTRASOUND TECHNOLOGY FOR FOOD PROCESSING, PRESERVATION AND EXTRACTION Histo-cytochemistry and scanning electron microscopy for studying spatial and temporal extraction of metabolites induced by ultrasound. Towards chain detexturation mechanism Microscopic imaging as a tool to target spatial and temporal extraction of bioactive compounds through ultrasound intensificationUltrason. Review of Alternative Solvents for Green Extraction of Food and Natural Green solvents for analytical chemistry." Thesis, Avignon, 2019. http://www.theses.fr/2019AVIG0715.
Повний текст джерелаWith recent trends in the increasing interest to environmental, economic and safety considerations,extraction techniques have largely focused on finding solutions with sustainable and green values toimplement in food processing, cosmetic and pharmaceutical industries. In this context, new “green”extraction techniques were developed such as Ultrasound-Assisted Extraction (UAE). The mainobjective of this thesis is industrial implementation of this new process in substitution to theconventional (CV) process. It has been shown in this work that the extraction of compounds ofinterest from rosemary and other plant matrices could be intensified using ultrasound, and thatdifferent performance gain could be achieved according to the plant matrix structural properties.Indeed, macroscopic and microscopic investigation of untreated and treated raw materials provedthat US act through different mechanisms and its resulting impacts can be extremely limited by plantstructural morphological and chemical properties, especially those of the specialized structures.Significant variability in performance gain was also observed at the industrial scale. Overall, USappears as a promising technique with a significant performance gain in terms of extraction yield andselectivity. Moreover, this process presents low environmental footprint compared to the CV one.Finally, it has been shown that natural products, such as honey and fruit juices, can be used toimprove solubilization and extraction of molecules that are poorly soluble in water. Encouragingresults were obtained in terms of solubilization and extraction abilities, especially from ground rawmaterials. However, these results raise questions related to the feasibility of industrialimplementation of this new process
Torres, Nogueira Luisa Maria. "Les lésions osseuses tranchantes (par scies) et tranchantes contondantes : analyse des mécanismes lésionnels et des instruments à l'origine de ces lésions." Thesis, Aix-Marseille, 2018. http://www.theses.fr/2018AIXM0272/document.
Повний текст джерелаIn this experimental work bone lesions produced by saws and a hatchet on human and animal samples were analyzed. With regard to the saws, 170 experimental false starts lesions were studied under stereomicroscope produced by five different saws. Universal saws behave like crosscut saws, because each tooth displays a tilt backwards. The minimum width of the kerf makes it possible to classify bone lesions according to Symes’ categories. Convex profiles indicate the use of a universal or crosscut saw. Concave profiles vary a great deal and indicate the use of a rip saw. The shape of the walls allows for determining the type of set except when they are straight or difficult to analyze. Among the secondary criteria, the appearance of the striae on the kerf floor is able to point the type of set. For the study of bone lesions by a hatchet a standardized device was used to produce small bone lesions. The stereomicroscope was able to observe the vertical striae explained by the vertical movement of the instrument at the time of impact. The scanning electron microscope allowed for a detailed analysis of bone lesions and made it possible to understand the uprising and the lateral pushing back. The presence of a lateral pushing back and of vertical striae is sufficient to determine that the bone lesions were achieved by a sharp blunt instrument. These characters are visible even after carbonization
Doran, Marc C. "Nanoindentation as a Characterization Tool for Wear Resistance in Stainless Steels." The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1462807632.
Повний текст джерелаRobson, Alex J. "Single particle tracking as a tool to investigate the dynamics of integrated membrane complexes in vivo." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:7769f80c-a56d-4513-9123-1d65ef8c9911.
Повний текст джерелаHohner, Robin, and Ekengren André. "Study Of Belts Acting As A Positioning System For Interconnected Gripping Tools In Tube Filling Machines." Thesis, Linnéuniversitetet, Institutionen för maskinteknik (MT), 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-77221.
Повний текст джерела