Дисертації з теми "Microscopy and tomography"
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Godavarthi, Charankumar. "Optical diffraction tomography microscopy : towards 3D isotropic super-resolution." Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM4337/document.
Повний текст джерелаThis PhD thesis is devoted to the three-dimensional isotropic resolution improvement using optical tomographic diffraction microscopy (TDM), an emerging optical microscope technique. The principle is to illuminate the sample successively with various angles of coherent light, collect the complex (amplitude and phase) diffracted field and reconstruct the sample 3D permittivity map through an inversion algorithm. A single TDM measurement was shown to combine several popular microscopy techniques such as bright-field microscope, dark-field microscope, phase-contrast microscope, confocal microscope, 2D and 3D synthetic aperture microscopes. All rely on scalar and linear approximations that assume a linear link between the object and the field diffracted by it, which limit their applicability to retrieve the object quantitatively. Thanks to a rigorous numerical inversion of the TDM diffracted field data which takes into account the polarization of the field and the multiple scattering process, we were able to reconstruct the 3D permittivity map of the object with a λ/4 transverse resolution. A further improvement to λ/10 transverse resolution was achieved by providing a priori information about the sample to the non-linear inversion algorithm. Lastly, the poor axial resolution in microscopes is due to the fundamental asymmetry of illumination and detection. To overcome this, a mirror-assisted tomography configuration was implemented, and has demonstrated a sub-λ/2 axial resolution capability. As a result, TDM can be seen as a powerful tool to reconstruct objects in three-dimensions with their optical material properties at resolution far superior to conventional microscopes
Bertilson, Michael. "Laboratory soft x-ray microscopy and tomography." Doctoral thesis, KTH, Biomedicinsk fysik och röntgenfysik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-29950.
Повний текст джерелаQC 20110221
Balakishan, Harishankar. "Nanoscale Tomography Based in Electrostatic Force Microscopy." Doctoral thesis, Universitat de Barcelona, 2021. http://hdl.handle.net/10803/671789.
Повний текст джерелаLa capacidad de caracterizar los elementos debajo de la superficie ha sido una necesidad imperiosa en los campos de la ciencia de los materiales, la tecnología de polímeros, la biología y las ciencias médicas. La microscopía de sonda de barrido (SPM por sus siglas en inglés) es una técnica de microscopía que permite exploran la superficie de una muestra a nano escala utilizando una sonda nanométrica, donde los datos adquiridos se utilizan para reconstruir las propiedades físicas de las muestras en resolución nanométrica (por ejemplo, topografía). Dado que las mediciones se pueden realizar sin contacto, los diferentes tipos de SPM se han convertido en candidatos óptimos para el estudio de propiedades sin necesidad de destruir la muestra. El SPM también posee la ventaja relativa de ser no invasivo, no destructivo, requiere una preparación de muestra relativamente sencilla, puede extenderse a cualquier ambiente (inerte, vacío ambiental), y también medirse en aire, agua o cualquier medio biológico. Entre ellos, la microscopía de fuerza electrostática, se ha utilizado con éxito en investigaciones del subsuelo para estudiar las modificaciones de composición debajo de las capas orgánicas, obtener imágenes debajo de las capas orgánicas, obtener imágenes de moléculas de agua confinada en canales nanométricos, imágenes de nanotubos de carbono, redes de grafeno y nanopartículas dentro de polímeros. Los nanocompuestos, que consisten en nanoestructuras en gran parte de su matriz para mejorar la eficiencia de la matriz, han sido una de las aplicaciones de la ciencia de materiales incorporadas con éxito en las últimas dos décadas. Las nanopartículas de plata tienen especialmente un aluvión de aplicaciones en su haber que van desde aplicaciones de células solares, pantallas táctiles, LED hasta dispositivos portátiles flexibles. Comprender las características del subsuelo o la tomografía de estos nanocompuestos podría ayudarnos a comprender sus propiedades, interpretándolas en función de su dependencia paramétrica, lo que luego nos ayudaría a ajustarlos para otras aplicaciones. En esta tesis, se han realizado estudios computacionales individuales de nano cables enterrados en una matriz dieléctrica para observar los efectos de varios parámetros que influyen en las imágenes del subsuelo. La resolución espacial tiene una importancia primordial, ya que se estudia su comportamiento de dos nano cables paralelos junto con dos nano cables superpuestos uno encima del otro. Además, el análisis de nanocompuestos de nano cables de plata se han investigado con la ayuda de la microscopía de barrido volumen de fuerza dieléctrica, una técnica propuesta recientemente con el EFM. La mayor parte de la matriz está compuesta de gelatina que puede ofrecer un rango de permitividades dependiendo del grado de hidratación, por ejemplo, aquí εr ~ 5 a εr ~ 14. Esta muestra se analiza experimentalmente, se obtienen imágenes y la profundidad de los nano cables en la matriz se mapean con el análisis teórico. Esta tesis nos proporciona nueva información y técnicas avanzadas a nivel tomográfico que ayudaran a la realización de imágenes de nanoestructuras de nuevos nanomateriales para aplicaciones en Salud y Electrónica.
Niehle, Michael. "Electron tomography and microscopy on semiconductor heterostructures." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät, 2016. http://dx.doi.org/10.18452/17607.
Повний текст джерелаElectron tomography exhibits a very poor spread in the research field of epitaxial semiconductor heterostructures in spite of the ongoing miniaturization and increasing three-dimensional (3D) character of nano-structured devices. This necessitates a tomographic approach at the nanometre scale in order to characterize and understand the relation between structure and physical properties of respective material systems. The present work demonstrates the rigorous application of electron tomography to an III-Sb based laser and to an (In,Ga)N/GaN nanocolumn heterostructure. A specific target preparation using a versatile FIB-SEM dual-beam microscope is emphasized as indispensable. The purposeful orientation of the specimen during preparation and the careful selection of an imaging mode in the scanning-/transmission electron microscope (S/TEM) are regarded in great detail. The comprehensive spatial microstructure characterization of the antimonide based heterostructure follows the dimensionality of crystal defects. The facetting and position of a pore (3D defect) which is unexpected in the MBE grown GaSb layer, is determined. The interplay of the initially grown AlSb islands on Si, the formation of a misfit dislocation network at the heterostructure interface (2D defect) and the presence of threading dislocations is investigated by the correlation of tomographic and complementary S/TEM results. The spatial arrangement of dislocations (1D defects) penetrating the whole stack of antimonide layers is revealed by electron tomography. The interaction of these line defects with anti-phase boundaries and with other dislocations is exclusively observed in the 3D result. The insertion of (In,Ga)N into oblique GaN nanocolumns is uniquely accessed by electron tomography. The amount of incorporated indium and the (In,Ga)N layer thickness is shown to vary on the different facets of the GaN core.
Ford, Bridget K. "Computed tomography based spectral imaging for fluorescence microscopy." Diss., The University of Arizona, 2002. http://hdl.handle.net/10150/280122.
Повний текст джерелаSwinford, Richard William. "An AFM-SIMS Nano Tomography Acquisition System." PDXScholar, 2017. https://pdxscholar.library.pdx.edu/open_access_etds/3485.
Повний текст джерелаSelin, Mårten. "3D X-ray microscopy: image formation, tomography and instrumentation." Doctoral thesis, KTH, Biomedicinsk fysik och röntgenfysik, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-184095.
Повний текст джерелаTomografi i mjukröntgenmikroskopi är en ny teknik för att få ut kvantitativ strukturell 3D information om celler. Dess styrka jämfört med andra tekniker är att den kan avbilda intakta celler i deras nära naturliga tillstånd med ett par 10 nm upplösning, utan omfattande preparering. Dock är metoderna för att rekonstruera 3D-data beroende av algoritmer som antar projektionsdata, vilket bilderna i allmänhet inte är på grund av avbildningsystemens begränsade skärpedjup. För att få ut den fulla potentialen av tomografi i röntgenmikroskopi behövs en ökad förståelse för avbildningsprocessen. Denna avhandling behandlar zonplatte-baserad röntgenmikroskopi för biologisk avbildning och den nödvändiga teorin för en numerisk implementering av en avbildningsmodell i 3D. En ny rekonstruktionsmetod föreslås som förbättrar upplösningen i rekonstruktionen för ett tomografiskt avbildat objekt. Detta visas i simuleringar och experiment. Slutligen omfattar denna avhandling arbete på Stockholms mjukröntgenmikroskop, inklusive en uppgradering av röntgenkällan som ger oöverträffad ljusstyrka för ett kompakt system. Denna uppgradering möjliggör högkvalitativ avbildning av celler i deras nästan naturliga tillstånd med endast 10 sekunders exponering.
QC 20160324
Sharp, Joanne. "Electron tomography of defects." Thesis, University of Cambridge, 2010. https://www.repository.cam.ac.uk/handle/1810/228638.
Повний текст джерелаMazlin, Viacheslav. "Tomographie optique cohérente pour l’imagerie in vivo de la cornée." Thesis, Paris Sciences et Lettres (ComUE), 2019. http://www.theses.fr/2019PSLET024.
Повний текст джерелаThis PhD project aimed to create an optical system for non-contact cellular resolution imaging of the human cornea in vivo. To achieve that, the contact ex vivo time-domain full-field optical coherence tomography (FFOCT) system was transformed into a non-contact in vivo imaging device and was for the first time applied to the human eye. FFOCT acquired images from the entire human cornea, limbus, sclera and tear film, revealing cells and nerves, which could be quantified over a millimetric field-of-view, beyond the capability of confocal microscopy and conventional optical coherence tomography (OCT). Blood flow and tear film dynamics could be directly followed and quantified. Furthermore, FFOCT was combined with a conventional OCT to perform real-time axial eye tracking and defocusing correction. The latter enabled real-time FFOCT imaging and display, which opens a path for future device implementation in clinical research and practice. Bench to bedside transfer of FFOCT is further stimulated by several solutions proposed in the manuscript, aiming to reduce the instrumentational complexity. Finally, a related FFOCT device was applied to imaging in vivo human retina, revealing the photoreceptors
Xiao, Juan. "Development of electron tomography on liquid suspensions using environmental scanning electron microscopy." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSEI050/document.
Повний текст джерелаESEM (Environmental Scanning Electron Microscopy) allows the observation of liquids under specific conditions of pressure and temperature. When working in the transmission mode, i.e. in STEM (Scanning Transmission Electron Microscopy), nano-objects can even be analyzed inside the liquid (“wet-STEM” mode). Moreover, in situ evaporation of water can be performed to study the materials evolution from the wet to the dry state. This work aims at developing electron tomography on liquid suspensions using STEM-in-ESEM, to obtain the 3D structure of nano-objects dispersed in a liquid. In a first part, Monte Carlo simulations and 2D wet-STEM experimental images are combined to study the contrast. Two kinds of liquid nano-materials are chosen as the sample: spherical gold particles (diameter around 40 nm) in suspension in water; latex SBA-PMMA suspension, a copolymer derived from styrene and metacrylic acid esters in aqueous solution, 3% PMMA shell included as steric surfactant. The comparison between simulated and experimental results helps to determine how water can affect the contrast of hydrated nano-materials. Tomography experiments are then performed on dry PU-carbon nanotubes nanocomposites using a previously developed home-made tomography device, and the volume is well reconstructed. When performing tomography on latex suspension, limitations are found on the temperature control of samples. We propose an optimization of the device with new observations conditions to better control water evaporation and condensation of liquid samples. Afterwards, a full 3D analysis on SBA-PMMA latex from dilute suspension to very concentrated one is performed, and a further study is presented in presence of a surfactant. The encouraging reconstruction results are used to model the particles arrangement. This shows the potentialities of wet-STEM tomography for the characterization of both solid and liquid nano-materials
Pan, Zhipeng. "Advanced optical microscopy for three dimensional deformation, profile and tomography measurement." Thesis, Georgia Institute of Technology, 2016. http://hdl.handle.net/1853/54908.
Повний текст джерелаKrehl, Jonas. "Incorporating Fresnel-Propagation into Electron Holographic Tomography." Master's thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-217919.
Повний текст джерелаTomographische Elektronenholographie kombiniert Tomographie, die Rekonstruktion dreidimensional aufgelößter Daten aus einem Satz von mehreren Messungen bei verschiedenen Objektorientierungen, mit Elektronenholographie, eine interferrometrische Messung der komplexen Elektronenwelle im Transmissionselektronenmikroskop (TEM). Wegen Mehrfachstreuung und Propagationseffekten erzeugt konventionelle, auf einer Strahlprojektion basierende, Tomography ernste Probleme bei Hochauflösung hin zu atomarer Auflösung. Diese sollen durch ein Modell, welches Fresnel-Propagation beinhaltet, aber weiterhin linear im Potential des Objektes ist, vermindert werden. Mit dem Rytov-Ansatz wird eine Näherung abgeleitet, wobei der Logarithmus der komplexen Welle linear im Potential ist. Die Strahlen-Projektion ist dann eine Faltung mit dem Fresnel-Propagations-Faltungskernel welche rechentechnisch wesentlich aufwendiger ist. Ein Programm-Paket für solche Rechnungen wurde in Python implementiert. Weiterhin wurde ein Multislice Algorithmus für große Gesichtsfelder und Objekte mit vielen Atomen wie Nanopartikel optimiert. Die Rytov-Näherung verbessert sowohl die Auflösung als auch die Signalqualität immens gegenüber konventioneller Tomographie, zumindest in dem getesteten System eines Wolframdisulfid-Nanoröhrchens. Das Rauschverhalten scheint ähnlich der konventionallen Tomographie zu sein, also eher gutmütig. Im Gegenzug braucht die Tomographie basierend auf der Rytov-Näherung wesentlich mehr Rechenzeit pro Iteration
Sandin, Sara. "Cryo-electron tomography of individual protein molecules /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-462-7/.
Повний текст джерелаRen, Christopher Xiang. "Multi-microscopy characterisation of III-nitride devices and materials." Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/264158.
Повний текст джерелаRisi, Matthew D. "Advances In Combined Endoscopic Fluorescence Confocal Microscopy And Optical Coherence Tomography." Diss., The University of Arizona, 2014. http://hdl.handle.net/10150/332772.
Повний текст джерелаVitry, Pauline. "Applications and development of acoustic and microwave atomic force microscopy for high resolution tomography analysis." Thesis, Dijon, 2016. http://www.theses.fr/2016DIJOS046/document.
Повний текст джерелаThe atomic force microscope (AFM) is a powerful tool for the characterization of organic and inorganic materials of interest in physics, biology and metallurgy. However, conventional scanning probe microscopy techniques are limited to the probing surface properties, while the subsurface analysis remains difficult beyond nanoindentation methods. Thus, the present thesis is focused on two novel complementary scanning probe techniques for high-resolution volumetric investigation that were develop to tackle this persisting challenge in nanometrology. The first technique considered, called Mode Synthesizing Atomic Force Microscopy (MSAFM), has been exploited in collaboration with Dr. Laurene Tetard of University of Central Florida to explore the volume of materials with high spatial resolution by means of mechanical actuation of the tip and the sample with acoustic waves of frequencies in the MHz range. A comprehensive study of the impact of the frequency parameters on the performance of subsurface imaging has been conducted through the use of calibrated samples and led to the validation of a numerical model for quantitative interpretation. Furthermore, this non-invasive technique has been utilized to locate lipid vesicles inside bacteria (in collaboration with Pr. A. Dazzi and M.-J. Virolle of Université Paris Sud, Orsay). Furthermore, we have combined this ultrasonic approach with infra-red microscopy, to add chemical speciation aimed at identifying the subsurface features, which represents a great advance for volume and chemical characterization of biological samples. The second technique considered is the Scanning Microwave Microscopy, which was developed in collaboration with Keysight society. Similar to acoustic-based microscopy, this non-invasive technique provided physical and chemical characterizations based on the interaction of micro-waves radiations with the matter (with frequency ranging from 0.2 and 16 GHz). Particularly, for metallic samples we performed volumetric characterization based on the skin effect of the materials. On the other hand, we have used this technique to analyze the diffusion of light chemical elements in metals and measured the effect of changes in mechanical properties of materials on their conductivity.Overall, these results constitute a new line of research involving non-destructive subsurface high resolution analysis by means of the AFM of great potential for several fields of research
Zhou, Yifeng. "Design and application of combined multiphoton microscopy and optical coherence tomography system." Thesis, University of British Columbia, 2012. http://hdl.handle.net/2429/43195.
Повний текст джерелаNam, Ahhyun. "Development and translation of label-free functional microscopy based on optical coherence tomography." Thesis, Massachusetts Institute of Technology, 2017. http://hdl.handle.net/1721.1/108851.
Повний текст джерелаThis electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Cataloged from student-submitted PDF version of thesis.
Includes bibliographical references.
Optical coherence tomography (OCT), an imaging modality based on low coherence interferometry, can be extended to obtain various endogenous functional contrasts. This thesis focuses on the development and translation of angiographic and polarization sensitive (PS) OCT techniques for clinical and preclinical applications. This goal includes four specific aims. The first aim is to develop a clinical imaging system to image the anatomy and microvasculature of human skin. The second aim is to develop a high performance post-processing algorithm for angiographic OCT. Towards this aim, we developed a processing algorithm based on complex differential variance (CDV) and confirmed its performance by benchmarking it against other published algorithms. The third aim is to develop a new approach for achieving high spatial resolution with an extended depth of focus for angiographic imaging. To achieve this aim, we have designed and built a triband wavelength system in which each spectrum is tightly focused at displaced focal planes to yield high transverse resolution over an effectively extended depth range. The fourth aim is to provide an imaging platform for preclinical study of peripheral nerve injury and repair. The vascularization is assessed by angiographic OCT, and the degree of myelination is measured by PS-OCT. These results confirm that the OCT platform can reveal new insights into preclinical studies of nerve regeneration and may ultimately provide a means for clinical intraoperative assessment of peripheral nerve health.
by Ahhyun Stephanie Nam.
Ph. D.
Aguirre, Aaron Dominic 1977. "Advances in Optical Coherence Tomography and Microscopy for endoscopic applications and functional neuroimaging." Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/43740.
Повний текст джерелаThis electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Includes bibliographical references.
Optical Coherence Tomography (OCT) is a developing medical imaging technology that generates micron resolution cross-sectional images of subsurface internal tissue structure in situ and in real time, without the need to remove and process specimens. Previous studies have suggested that OCT holds great potential for use in laparoscopic and endoscopic applications to detect early stage neoplastic pathologies. A minimally invasive imaging modality capable of identifying pre-malignant tissues in vivo could be used to guide conventional excisional biopsy and histology, thereby reducing sampling error and enabling earlier detection and treatment. One limitation of prior endoscopic OCT imaging methods is the inability to visualize cellular features characteristic of early disease states such as neoplasia. This thesis seeks to demonstrate that advances in OCT resolution and in miniaturized imaging devices will lead to enhanced visualization of pathologic changes in vivo at both the tissue architectural and cellular levels. Toward this goal, three technological advances are made. First, compact and portable laser light sources for clinical ultrahigh resolution OCT are demonstrated based on supercontinuum generation in highly nonlinear optical fibers. Second, an extension of OCT called optical coherence microscopy (OCM) is developed for in vivo cellular imaging. High speed OCM system designs are demonstrated and characterization of OCM imaging parameters is performed. Importantly, this work demonstrates that OCM can make use of broadband laser sources to image cellular features with reduced numerical aperture compared to confocal microscopy, thereby facilitating the development of small diameter endoscopic probes.
(cont.) Third, two-axis scanning catheters based on micromirror technology are designed and demonstrated for ultrahigh resolution three-dimensional and en face OCT imaging. To demonstrate feasibility of these advances in future clinical applications, ex vivo imaging studies of endoscopically accessible human gastrointestinal tissues including key pathologies are performed. Results demonstrate that three-dimensional and cellular resolution optical coherence imaging can significantly improve performance over conventional OCT methods for gastrointestinal endoscopy. Finally, this thesis also explores a new application for optical coherence tomography in neuroscience. Optical methods are currently being used to study the neurovascular response to functional activation, but most existing techniques lack depth resolution. Through correlation with video microscopy, OCT is shown to enable depth-resolved cross-sectional imaging of functional activation in the important rat somatosensory cortex model system. With further development, OCT may offer a new tool for basic and applied neuroscience research.
by Aaron Dominic Aguirre.
Ph.D.
Müller, Paul. "Optical Diffraction Tomography for Single Cells." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-202261.
Повний текст джерелаButts, Mark D. "Nondestructive examination of nicalon fiber composite preforms using x-ray tomographic microscopy." Thesis, Georgia Institute of Technology, 1993. http://hdl.handle.net/1853/19959.
Повний текст джерелаZhang, Daliang. "3D Electron crystallography real space reconstruction and reciprocal space tomography /." Doctoral thesis, Stockholm : Department of Materials and Environmental Chemistry (MMK), Stockholm University, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-39034.
Повний текст джерелаChen, Yi-Sheng. "Characterisation of hydrogen trapping in steel by atom probe tomography." Thesis, University of Oxford, 2017. http://ora.ox.ac.uk/objects/uuid:9d8ee66f-176d-4ac1-aad6-ccb33efc924d.
Повний текст джерелаLiu, Yang. "‘Tri-3D’ electron microscopy tomography by FIB, SEM and TEM : Application to polymer nanocomposites." Thesis, Lyon, INSA, 2013. http://www.theses.fr/2013ISAL0076/document.
Повний текст джерелаThis work is focused on the characterization and quantification of the 3D distribution of different types of fillers (nanoparticles, nanotubes, etc.) in polymer matrices. We have essentially used tomography techniques in electron microscopy. Multiple approaches to electron tomography were performed: FIB-SEM (focused ion beam/scanning electron microscope) tomography, SEM tomography and TEM (transmission electron microscope) tomography. Polymer nanocomposites are basically synthesized in order to improve the physical properties (mechanical, electric, etc.) of the pure polymer constituting the matrix, by a controlled addition of fillers at the nanoscale. The characterization of such materials and the establishment of accurate correlations between the microstructure and the modified properties require a three-dimensional approach. According to the nanometric size of the fillers, electron microscopy techniques are needed. Two systems of polymer nanocomposites have been studied by multiple electron tomography approaches: P(BuA-stat-S)/MWNTs (statistical copolymer poly(styrene-co-butyl acrylate) reinforced by multi-walled carbon nanotubes) and P(BuA-stat-MMA)/SiO2 (statistical copolymer poly(butyl acrylate-co-methyl methacrylate) reinforced by silica nanoparticles). By combining various techniques, the characterization and the quantification of nanofillers were possible. In particular, statistics about size, distribution and volume fraction of the fillers were measured. This study has then provided 3D information, which contributes to a better understanding of properties of the nanocomposites. Attention has been paid to analyze carefully original data, and artifacts and causes of errors or inaccuracy were considered in the 3D treatments. We also attempted to compare benefits and drawbacks of all techniques employed in this study, and perspectives for future improvements have been proposed
Bennett, Samantha. "Nitride semiconductors studied by atom probe tomography and correlative techniques." Thesis, University of Cambridge, 2011. https://www.repository.cam.ac.uk/handle/1810/236685.
Повний текст джерелаFederici, Antoine. "Développement de systèmes de microscopie par cohérence optique plein champ étendus spatialement et spectralement." Thesis, Université Paris-Saclay (ComUE), 2015. http://www.theses.fr/2015SACLS024/document.
Повний текст джерелаFull-field optical coherence tomography (FF-OCT) is an optical technology based on low-coherence interference microscopy for tomographic imaging of semitransparent samples. Non-invasive three-dimensional imaging can be performed with an isotropic spatial resolution of the order of 1 µm. During the PhD thesis, several FF-OCT systems have been reported achieving extended performances or contrast enhanced images relevant for biological tissues imaging. Firstly, a three-band, 1.9-μm axial resolution FF-OCT system has been implemented to perform spectroscopic contrast enhanced imaging of biological tissues over a 530-1700 nm wavelength range. Then, a study of the FF-OCT axial response has been carried out for maximizing the axial resolution of the system. An isotropic spatial resolution of 0.5 µm (in water) has been obtained by combining 1.2-NA microscope objectives with an optimized broad spectral band adapted to biological tissues imaging, such as skin samples. A set-up with an extended field of view of 18 mm x 18 mm has been also designed and applied to amplitude signal detection as well as depth-resolved quantitative phase signal measurement. At last, we developed a technique based on the combination of full-field swept-source optical coherence tomography (FF-SSOCT) with low spatial coherence illumination and a special numerical processing that allows for numerically focused mechanical motion-free three-dimensional imaging
Fogelqvist, Emelie. "Laboratory Soft X-Ray Cryo Microscopy: Source, System and Bio Applications." Doctoral thesis, KTH, Biomedicinsk fysik och röntgenfysik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-206428.
Повний текст джерелаQC 20170505
Eavis, Joe. "An investigation of soft tissue ultrasonic microimaging." Thesis, University of Bristol, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310800.
Повний текст джерелаSu, Zhongyi. "Performance enhancement of all-solid-state batteries by optimizing the electrolyte through advanced microscopy and tomography techniques." Thesis, The University of Sydney, 2020. https://hdl.handle.net/2123/22112.
Повний текст джерелаBarnett, Brett William. "RECONSTRUCTION TECHNIQUES FOR OBTAINING ARTERIAL CONTOURS FROM DATA ACQUIRED IN VIVO (BLOOD FLOW, MICROSCOPY, TOMOGRAPHY)." Thesis, The University of Arizona, 1986. http://hdl.handle.net/10150/275488.
Повний текст джерелаJahr, Wiebke. "Spectrally resolved, three-dimensional widefield microscopy." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-225963.
Повний текст джерелаEin wichtiges Ziel biologischer Bildgebung ist die Visualisierung des Zusammenspiels von verschiedenen, meist fluoreszent markierten, Geweben bei dynamischen Prozessen. Nur wenige dieser Farbstoffe passen ohne Überlapp in das zur Verfügung stehende Spektrum. Sie können jedoch rechnerisch getrennt werden, wenn das gesamte Spektrum jedes Pixels bekannt ist. In medizinischen Anwendungen versprechen hyperspektrale Techniken, verschiedene Gewebetypen markierungsfrei zu identifizieren. Dennoch ist es in der Mikroskopie noch immer üblich, spektrale Information entweder mit Filtern über breiten Bändern zu integrieren, oder Punktspektren mithilfe von Dispersion zu trennen und auf einem Multikanaldetektor aufzunehmen, was inhärent langsam ist. Light Sheet Fluorescence Microscopy (LSFM) und Optical Projection Tomography (OPT) nehmen 3D Mikroskopiedaten schnell, photoneneffizient und sanft für die Probe auf. LSFM arbeitet mit Fluoreszenz, OPT in Transmission. Beide basieren auf schneller Weitfelddetektion, wobei die räumliche Information mit einem 2D Detektor aufgenommen wird, der keinen Raum lässt, um die getrennten Spektren zu messen. Hyperspektrale Bildgebung wurde bis jetzt für keine der zwei Techniken gezeigt. Ich habe ein hyperspektrales LSFM mit Linienabtastung und ein OPT mit Wellenlängenabtastung entwickelt, um 5D Daten (3D räumlich, 1D zeitlich, 1D spektral) aufzunehmen. Beide Aufbauten wurden hinsichtlich minimaler Aufnahmezeit optimiert, ohne dabei Kontrast, räumliche oder spektrale Auflösung zu opfern. Ich habe verschiedene Abläufe zum Klassifizieren und Trennen der Hauptkomponenten implementiert. Ich nehme bis zu fünf Fluorophore und Autofluoreszenz in Zebrafisch- und Fruchtfliegenembryos mit dem hyperspektralen LSFM auf und zeige die Effizienz des gesamten Ablaufes, indem ich Spektren und räumliche Verteilung aller Marker extrahiere. Die Transmissionsdaten des spektralen OPT werden mit denselben Methoden untersucht. Ich konnte belegen, dass die Bildformation im OPT massgeblich von Brechung bestimmt ist, und Streuung und Absorption nur einen geringen Beitrag leisten. Außerdem habe ich ein robustes, didaktisches LSFM gebaut, damit Laien die Funktionsweise moderner Mikroskopie erkunden können. Dieses eduSPIM war ein Jahr lang in den Technischen Sammlungen Dresden ausgestellt
Leary, Rowan Kendall. "Atomic-scale and three-dimensional transmission electron microscopy of nanoparticle morphology." Thesis, University of Cambridge, 2015. https://www.repository.cam.ac.uk/handle/1810/246903.
Повний текст джерелаSamudrala, Saritha Kowmudy. "Atomic scale analysis of nanocrystalline materials by advanced microscopy." Thesis, The University of Sydney, 2015. http://hdl.handle.net/2123/13655.
Повний текст джерелаParadiso, Nicola. "Tomography and manipulation of quantum Hall edge channels." Doctoral thesis, Scuola Normale Superiore, 2012. http://hdl.handle.net/11384/85830.
Повний текст джерелаBilal, Huma. "Atomic Scale Microscopy of Zr-based Bulk Metallic Glasses Processed by Various Routes." Thesis, The University of Sydney, 2022. https://hdl.handle.net/2123/29908.
Повний текст джерелаNiehle, Michael [Verfasser], Henning [Gutachter] Riechert, Eric [Gutachter] Tournié, and Günter [Gutachter] Möbus. "Electron tomography and microscopy on semiconductor heterostructures / Michael Niehle ; Gutachter: Henning Riechert, Eric Tournié, Günter Möbus." Berlin : Mathematisch-Naturwissenschaftliche Fakultät, 2016. http://d-nb.info/1116433931/34.
Повний текст джерелаLa, Fontaine Alexandre Jacques. "Understanding the structure of minerals at the atomic scale: a new perspective enabled by advanced microscopy." Thesis, The University of Sydney, 2016. http://hdl.handle.net/2123/15484.
Повний текст джерелаKoneti, Siddardha. "In situ and 3D environmental transmission electron microscopy of Pd-Al2O3 nano catalysts : Fast tomography with applications to other catalytic systems in operando conditions and to electron beam sensitive nanomaterials." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSEI123/document.
Повний текст джерелаIn the beginning of the XXIst century, Environmental Transmission Electron Microscopy has become one of the reliable characterization techniques of nanomaterials in conditions mimicking their real life. ETEM is now able to follow the dynamic evolution of nanomaterials under various conditions like high temperature, liquid or various gas pressures. Among various fields of research, catalysis can benefit significantly from Environmental Microscopy. This contribution starts with the study of the Palladium-Alumina catalytic system. Pd nanoparticles supported by α-Al2O3 and δ-Al2O3 are of an important physicochemical and environmental interest, particularly in the field of selective hydrogenation in petrochemistry, for the synthesis of polymers or CO2 hydrogenation for methane production. We first performed 2D analyses at different steps of the synthesis process, then the same synthesis steps were performed under in situ conditions. The motivation of this approach was to compare post mortem treatments with ETEM observations. In general, 2D data provide limited insights on, for example, the morphology and position of supported nanoparticles. We have then developed a new fast acquisition approach to collect tomographic tilt series in very short times, enabling to reconstruct nano-systems in 3D during their dynamical evolution. Taking advantage of this approach, we have determined the activation energy for soot combustion on YSZ oxidation catalysts for diesel motors from volumetric data extracted from in situ experiments. Fast electron tomography was also applied to electron beam sensitive materials, like polymer nanocomposites and biological materials, showing the wide spectrum of possible applications for rapid 3D characterization of nanomaterials
Grandfield, Kathryn. "Nanoscale Osseointegration : Characterization of Biomaterials and their Interfaces with Electron Tomography." Doctoral thesis, Uppsala universitet, Tillämpad materialvetenskap, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-179445.
Повний текст джерелаLiu, Xiaojing. "Optical Coherence Photoacoustic Microscopy (OC-PAM) for Multimodal Imaging." FIU Digital Commons, 2016. http://digitalcommons.fiu.edu/etd/3189.
Повний текст джерелаPerrot, Jean-Luc. "Explorations optiques multimodales et multiéchelles non invasives appliquées au revêtement cutanéomuqueux , étendues à l'appareil oculaire antérieur." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSES010/document.
Повний текст джерелаAfter a brief introduction to the history of non-invasive dermatological imaging, this work is divided into 3 parts. 1) Presentation of a project for the development of a low-cost miniaturized optical coherence tomograph to allow dissemination of this technique to dermatologists practicing outside hospitals. This is an ANR project: DOCT-VCSEL Portable Optical Coherence Tomography with MEMS-VCSEL swept-sources for skin analysis ANR 2015 / Societal Challenge "Life, Health and Welfare" Axis 13 “Technologies for Health" 2) Presentation of a project whose goal is the identification of cancer skin lesions by means of a new high definition OCT developed by the company DAMAE, resulting from the Higher Institute of Optics of Palaiseau. It is a device that will initially be reserved for centers of excellence in dermatological imaging. 3) Presentation of 52 publications related to skin imaging, in which I participated, and referenced in the international databases as of December 31, 2016. This work covers all modern dermatological non-invasive imaging and addresses Subjects that had never been studied in this way. Notably the mucous membranes and the anterior ocular apparatus but also the identification by confocal microscopy of the surgical margins or the association confocal microscopy Raman spectrometry
Ogien, Jonas. "Développement de systèmes de microscopie par cohérence optique pour l'imagerie de la peau." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLO011/document.
Повний текст джерелаOptical coherence microscopy (OCM) is a technique for tomographic imaging based on white light interferometry, making it possible to image biological media with micrometer-scale spatial resolution. OCM is particularly well-suited to dermatological imaging, especially skin cancer diagnosis, since it provides images that are similar to histological images without the need for biopsy.This PhD thesis focuses on the development of OCM for skin imaging, with the aim of providing a compact, in vivo imaging tool for the dermatologist, capable of acquiring structural and functional images of the skin.A compact, full-field OCM (FF-OCM) system illuminated by a white LED was first developed, making it possible to obtain tomographic images at an ultra-high resolution (0.7 μm × 1.8 μm), up to ∼200 μm in depth within the skin. Using a high power LED, in vivo skin images could be obtained.Using this FF-OCM setup, functional imaging methods for blood flow mapping (angiography) were implemented. Four methods, based on temporal or frequency analysis of the interferometric signal, phase images or amplitude images, have been shown to be able to image intralipid flow within a model blood capillary.Functional polarimetric imaging has also been explored in FF-OCM. Contrast optimization in polarimetric images has been obtained by modifying the polarizing components of the conventional polarization sensitive FF-OCM setup depending on the sample to be imaged. This method has been tested on a simple polarizing sample.Finally, a new OCM method, line-field confocal OCM (LC-OCM), has been studied. The goal here was to develop a system capable of imaging the skin in vivo, with a tissue penetration depth greater than what is possible for FF-OCM. This system, which combines interferometric filtering and confocal filtering, makes it possible to obtain in vivo skin images in vertical and en face slices, with a spatial resolution similar to that of FF-OCM, but with a greater penetration depth of 300 μm
Barbieri, Francesco. "INVESTIGATING THE FUNCTIONAL MORPHOLOGY OF IN SITU IFT TRAINS BY CORRELATIVE LIGHT-ELECTRON MICROSCOPY." Doctoral thesis, Università di Siena, 2018. http://hdl.handle.net/11365/1052316.
Повний текст джерелаCimalla, Peter, Theresa Werner, Kai Winkler, Claudia Mueller, Sebastian Wicht, Maria Gaertner, Mirko Mehner, et al. "Imaging of nanoparticle-labeled stem cells using magnetomotive optical coherence tomography, laser speckle reflectometry, and light microscopy." SPIE, 2015. https://tud.qucosa.de/id/qucosa%3A35268.
Повний текст джерелаBeasley, Daniel. "3D quantitative elemental mapping of biological tissues using proton induced X-ray emission tomography (PIXE-T) and on/off-axis scanning transmission ion microscopy tomography (STIM-T)." Thesis, University of Surrey, 2006. http://epubs.surrey.ac.uk/843466/.
Повний текст джерелаMoebel, Emmanuel. "New strategies for the identification and enumeration of macromolecules in 3D images of cryo electron tomography." Thesis, Rennes 1, 2019. http://www.theses.fr/2019REN1S007/document.
Повний текст джерелаCryo electron tomography (cryo-ET) is an imaging technique capable of producing 3D views of biological specimens. This technology enables to capture large field of views of vitrified cells at nanometer resolution. These features allow to combine several scales of understanding of the cellular machinery, from the interactions between groups of proteins to their atomic structure. Cryo-ET therefore has the potential to act as a link between in vivo cell imaging and atomic resolution techniques. However, cryo-ET images suffer from a high amount of noise and imaging artifacts, and the interpretability of these images heavily depends on computational image analysis methods. Existing methods allow to identify large macromolecules such as ribosomes, but there is evidence that the detections are incomplete. In addition, these methods are limited when searched objects are smaller and have more structural variability. The purpose of this thesis is to propose new image analysis methods, in order to enable a more robust identification of macromolecules of interest. We propose two computational methods to achieve this goal. The first aims at reducing the noise and imaging artifacts, and operates by iteratively adding and removing artificial noise to the image. We provide both mathematical and experimental evidence that this concept allows to enhance signal in cryo-ET images. The second method builds on recent advances in machine learning to improve macromolecule localization. The method is based on a convolutional neural network, and we show how it can be adapted to achieve better detection rates than the current state-of- the-art
Moore, Chad Lewis. "Insights into the three-dimensional ultrastructure of uterine epithelial cells during pregnancy." Thesis, The University of Sydney, 2020. https://hdl.handle.net/2123/22010.
Повний текст джерелаKorde, Vrushali Raj. "OPTICAL IMAGING MODALITIES: FROM DESIGN TO DIAGNOSIS OF SKIN CANCER." Diss., The University of Arizona, 2009. http://hdl.handle.net/10150/193716.
Повний текст джерелаKong, Jun. "A Study of Computer Vision and Pattern Recognition in Medical Image Analysis: Digital Microscopy and Optical Coherent Tomography." Columbus, Ohio : Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1228075056.
Повний текст джерелаRobles, Victor Adrian. "Automated image analysis of corneal structures in anterior-segment optical coherence tomography and in-vivo confocal microscopy images." Diss., University of Iowa, 2017. https://ir.uiowa.edu/etd/5988.
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