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Статті в журналах з теми "MicroCAD"

Автор: Grafiati

Опубліковано: 30 травня 2022

Оновлено: 31 травня 2022

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1

Koo, T. K., and Y. B. Aw. "Digital Charts for Marine Construction Activities: MicroCAD Approach." Journal of Surveying Engineering 116, no. 1 (February 1990): 30–41. http://dx.doi.org/10.1061/(asce)0733-9453(1990)116:1(30).

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2

Sato, Kazuo, Kazuo Asaumi, Gen Kobayashi, Yasuroh Iriye, and Mitsuhiro Shikida. "Development of an orientation-dependent anisotropic etching simulation system MICROCAD." Electronics and Communications in Japan (Part II: Electronics) 83, no. 4 (April 2000): 13–22. http://dx.doi.org/10.1002/(sici)1520-6432(200004)83:4<13::aid-ecjb2>3.0.co;2-l.

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3

Adeli, Hojjat, and James Fiedorek. "A MICROCAD system for design of steel connections—II. Applications." Computers & Structures 24, no. 3 (January 1986): 361–74. http://dx.doi.org/10.1016/0045-7949(86)90313-5.

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4

Adeli, Hojjat, and James Fiedorek. "A MICROCAD system for design of steel connections—I. Program structure and graphic algorithms." Computers & Structures 24, no. 2 (January 1986): 281–94. http://dx.doi.org/10.1016/0045-7949(86)90286-5.

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5

Eskerud, Ingeborg, Eva Gerdts, Terje H. Larsen, and Mai Tone Lønnebakken. "Left ventricular hypertrophy contributes to Myocardial Ischemia in Non-obstructive Coronary Artery Disease (the MicroCAD study)." International Journal of Cardiology 286 (July 2019): 1–6. http://dx.doi.org/10.1016/j.ijcard.2019.03.059.

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6

Chen, Richard. "Microcap Deception." Journal of Investing 9, no. 3 (August 31, 2000): 19–24. http://dx.doi.org/10.3905/joi.2000.319378.

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7

Wijaya, Andre, and Muhammad Adi Pribadi. "Interaksi Simbolik dalam Perencanaan Komunikasi Pemasaran PT. Microad Indonesia (Studi Etnografi: Systema Solution di Media Sosial)." Prologia 4, no. 2 (October 1, 2020): 402. http://dx.doi.org/10.24912/pr.v4i2.6712.

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Анотація:

Marketing communication activities carried out to be able to increase sales and introduce a brand to the wider community, in the current technological era social media plays an important role in conducting marketing communication. Lots of people use social media, this is an opportunity to do marketing communication through social media. PT. MicroAd Indonesia is an advertising company engaged in digital. PT. MicroAd Indonesia has its own way of marketing communication that is different from other advertising companies. Symbolic interactions also affect the marketing communication of PT. MicroAd Indonesia. This research uses a qualitative methodology with ethnographic methods. Data collection was carried out using participant observation, in-depth interviews with several teams at PT. MicroAd Indonesia, and also document analysis. The conclusion of this study is that there are several stages of marketing communication planning consisting of first meeting with clients, Kick Off, Doing research such as searching for key messages, Content Pillar and Content Mapping and then creating content for on social media such as Instagram, Facebook and Twitter to the final stage that is Monitoring.Kegiatan komunikasi pemasaran dilakukan untuk dapat meningkatkan penjualan dan memperkenalkan sebuah merek kepada masyarakat luas, di era teknologi saat ini media sosial sangat berperan penting dalam melakukan komunikasi pemasaran, Banyak sekali orang-orang yang menggunakan media sosial, ini merupakan suatu kesempatan untuk melakukan komunikasi pemasaran melalui media sosial. PT. MicroAd Indonesia adalah suatu perusahaan periklanan yang bergerak dibidang digital. PT. MicroAd Indonesia memiliki cara tersendiri untuk melakukan komunikasi pemasaran yang berbeda dengan perusahaan periklanan lainnya. Interaksi simbolik juga mempengaruhi komunikasi pemasaran PT. MicroAd Indonesia. Penelitian ini menggunakan metodologi kualitatif dengan metode etnografi. Pengumpulan data dilakukan dengan menggunakan observasi partisipan, wawancara mendalam dengan beberapa tim di PT. MicroAd Indonesia, dan juga analisis dokumen. Kesimpulan dari penelitian ini yaitu terdapat beberapa tahapan perencanaan komunikasi pemasaran yang terdiri dari pertemuan pertama dengan klien, Kick Off, Melakukan riset seperti mencari key message, Content Pillar dan Content Mapping lalu pembuatan konten untuk di media sosial seperti Instagram, Facebook dan Twitter hingga ketahap akhir yaitu adalah Monitoring.

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8

Delgado, Mónica A., José O. Solbiati, María J. Chiuchiolo, Ricardo N. Farías, and Raúl A. Salomón. "Escherichia coli Outer Membrane Protein TolC Is Involved in Production of the Peptide Antibiotic Microcin J25." Journal of Bacteriology 181, no. 6 (March 15, 1999): 1968–70. http://dx.doi.org/10.1128/jb.181.6.1968-1970.1999.

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ABSTRACT A Tn5 insertion in tolC eliminated microcin J25 production. The mutation had little effect on the expression of the microcin structural gene and presumably acted by blocking microcin secretion. The tolC mutants carrying multiple copies of the microcin genes were less immune to the microcin. TolC is thus likely a component of a microcin export complex containing the McjD immunity protein, an ABC exporter.

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9

Keller, Lorenzo, Anh Le, Blerim Cici, Hulya Seferoglu, Christina Fragouli, and Athina Markopoulou. "MicroCast." ACM SIGMOBILE Mobile Computing and Communications Review 16, no. 4 (February 4, 2013): 24–25. http://dx.doi.org/10.1145/2436196.2436210.

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10

Koenig, William D. B. "Analysis of Microcap Securities." AIMR Conference Proceedings 1997, no. 1 (March 1997): 8–13. http://dx.doi.org/10.2469/cp.v1997.n1.3.

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11

Rodríguez, Eliana, Carina Gaggero, and Magela Laviña. "The Structural Gene for Microcin H47 Encodes a Peptide Precursor with Antibiotic Activity." Antimicrobial Agents and Chemotherapy 43, no. 9 (September 1, 1999): 2176–82. http://dx.doi.org/10.1128/aac.43.9.2176.

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ABSTRACT Microcin H47 is a bactericidal antibiotic produced by a naturally occurring Escherichia coli strain isolated in Uruguay. The microcin genetic system is located in the chromosome and extends over a 10-kb DNA segment containing the genes required for microcin synthesis, secretion, and immunity. The smallest microcin synthesis gene,mchB, was sequenced and shown to encode a highly hydrophobic peptide. An mchB-phoA gene fusion, which directed the synthesis of a hybrid bifunctional protein with both PhoA and microcin H47-like activities, was isolated. The results presented herein lead us to propose that microcin H47 is indeed a ribosomally synthesized peptide antibiotic and that its peptide precursor already has antibiotic activity of the same specificity as that of mature microcin.

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12

Rodríguez, Eliana, and Magela Laviña. "Genetic analysis of microcin H47 immunity." Canadian Journal of Microbiology 44, no. 7 (July 1, 1998): 692–97. http://dx.doi.org/10.1139/w98-044.

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Microcin H47 is a bactericidal antibiotic produced by a natural Escherichia coli isolate. The genetic system encoding microcin production and immunity consists of at least seven clustered genes. Four of these are devoted to microcin biosynthesis and two genes are required for its secretion into the extracellular medium. The product of the seventh gene, mchI, determines the cell's self-immunity. This gene was shown to encode a highly hydrophobic 69-residue peptide. Analysis of the MchI amino acid sequence, as well as the characterization of MchIPhoA hybrid proteins, indicated that the microcin immunity product is probably exported out of the cytoplasm and remains an integral membrane peptide. This localization of the immunity peptide points to the cellular membrane as the site of action of microcin H47. Key words: microcin H47, microcin immunity, phoA gene fusion, membrane peptide.

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13

Lagos, Rosalba, Jorge E. Villanueva, and Octavio Monasterio. "Identification and Properties of the Genes Encoding Microcin E492 and Its Immunity Protein." Journal of Bacteriology 181, no. 1 (January 1, 1999): 212–17. http://dx.doi.org/10.1128/jb.181.1.212-217.1999.

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ABSTRACT The gene coding for the immunity protein (mceB) and the structural gene of microcin E492 (mceA), a low-molecular-weight channel-forming bacteriocin produced by a strain of Klebsiella pneumoniae, have been characterized. The microcin gene codes for a precursor protein of either 99 or 103 amino acids. Protein sequencing of the N-terminal region of microcin E492 unequivocally identified this gene as the microcin structural gene and indicated that this microcin is synthesized as a precursor protein that is cleaved at either amino acid 15 or 19, at a site resembling the double-glycine motif. The gene encoding the 95-amino-acid immunity protein (mceB) was identified by cloning the DNA segment that encodes only this polypeptide into an expression vector and demonstrating the acquisition of immunity to microcin E492. As expected, the immunity protein was found to be associated with the inner membrane. Analysis of the DNA sequence indicates that these genes belong to the same family as microcin 24, and they do not share structural motifs with any other known channel-forming bacteriocin. The organization of the microcin- and immunity protein-encoding genes suggests that they are coordinately expressed.

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14

Артюхов, В. Г., and И. В. Коноваленко. "Memristor circuit simulation in MicroCap." Electronics and Communications 20, no. 1 (July 23, 2015): 27. http://dx.doi.org/10.20535/2312-1807.2015.20.1.47384.

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15

Chen, Richard. "Precarious Investments in Microcap Stocks." Journal of Investing 13, no. 4 (November 30, 2004): 25–36. http://dx.doi.org/10.3905/joi.2004.450753.

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16

Lidozzi, A., L. Solero, and A. Di Napoli. "Ultracapacitors equipped hybrid electric MicroCar." IET Electric Power Applications 4, no. 8 (2010): 618. http://dx.doi.org/10.1049/iet-epa.2009.0096.

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17

Mercado, Gabriela, Mario Tello, Macarena Marín, Octavio Monasterio, and Rosalba Lagos. "The Production In Vivo of Microcin E492 with Antibacterial Activity Depends on Salmochelin and EntF." Journal of Bacteriology 190, no. 15 (May 23, 2008): 5464–71. http://dx.doi.org/10.1128/jb.00351-08.

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ABSTRACT Microcin E492 is a channel-forming bacteriocin that is found in two forms, namely, a posttranslationally modified form obtained by the covalent linkage of salmochelin-like molecules to serine 84 and an unmodified form. The production of modified microcin E492 requires the synthesis of enterochelin, which is subsequently glycosylated by MceC and converted into salmochelin. mceC mutants produced inactive microcin E492, and this phenotype was reversed either by complementation with iroB from Salmonella enterica or by the addition of exogenous salmochelin. Cyclic salmochelin uptake by Escherichia coli occurred mainly through the outer membrane catecholate siderophore receptor Fiu. The production of inactive microcin E492 by mutants in entB and entC was reverted by the addition of the end product of the respective mutated pathway (2,3-dihydroxybenzoic acid and enterochelin/salmochelin, respectively), while mutants in entF did not produce active microcin E492 in the presence of enterochelin or salmochelin. The EntF adenylation domain was the only domain required for this microcin E492 maturation step. Inactivation of the enzymatic activity of this domain by site-directed mutagenesis did not prevent the synthesis of active microcin E492 in the presence of salmochelin, indicating that the adenylation activity is not essential for the function of EntF at this stage of microcin E492 maturation.

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18

Vassiliadis, Gaëlle, Jean Peduzzi, Séverine Zirah, Xavier Thomas, Sylvie Rebuffat, and Delphine Destoumieux-Garzón. "Insight into Siderophore-Carrying Peptide Biosynthesis: Enterobactin Is a Precursor for Microcin E492 Posttranslational Modification." Antimicrobial Agents and Chemotherapy 51, no. 10 (October 2007): 3546–53. http://dx.doi.org/10.1128/aac.00261-07.

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ABSTRACT Microcin E492-producing bacteria secrete both unmodified and posttranslationally modified microcins. The modification consists of a C-glucosylated linear trimer of N-(2,3-dihydroxybenzoyl)-l-serine, a catecholate siderophore related to salmochelins and enterobactin. We show here that repression of enterobactin biosynthesis inhibits the acquisition of microcin E492 posttranslational modification, as monitored by high-performance liquid chromatography and mass spectrometry. Furthermore, exogenous enterobactin restored the production of posttranslationally modified microcin in a bacterial strain deficient in enterobactin synthesis. We thus concluded that enterobactin serves as a precursor for the synthesis of the posttranslationally modified microcin and that the unmodified microcin is an incompletely processed form of mature microcin E492. Gene disruption experiments showed that MceC and MceD, two enzymes encoded by the mceABCDEFGHIJ gene cluster, are involved in the synthesis of the microcin E492 posttranslational modification, as followed by mass spectrometry. Genes homologous to iroB and iroD, required for the conversion (linearization and C-glycosylation) of enterobactin into salmochelins, efficiently complemented mceC and mceD, respectively. Based on our results, a model is proposed for the biosynthesis of the mature siderophore-carrying peptide.

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19

Delgado, Mónica A., Paula A. Vincent, Ricardo N. Farías, and Raúl A. Salomón. "YojI of Escherichia coli Functions as a Microcin J25 Efflux Pump." Journal of Bacteriology 187, no. 10 (May 15, 2005): 3465–70. http://dx.doi.org/10.1128/jb.187.10.3465-3470.2005.

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ABSTRACT In the present study, we showed that yojI, an Escherichia coli open reading frame with an unknown function, mediates resistance to the peptide antibiotic microcin J25 when it is expressed from a multicopy vector. Disruption of the single chromosomal copy of yojI increased sensitivity of cells to microcin J25. The YojI protein was previously assumed to be an ATP-binding-cassette-type exporter on the basis of sequence similarities. We demonstrate that YojI is capable of pumping out microcin molecules. Thus, one obvious explanation for the protective effect against microcin J25 is that YojI action keeps the intracellular concentration of the peptide below a toxic level. The outer membrane protein TolC in addition to YojI is required for export of microcin J25 out of the cell. Microcin J25 is thus the first known substrate for YojI.

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20

Trujillo, Mónica, Eliana Rodrı́guez, and Magela Laviña. "ATP Synthase Is Necessary for Microcin H47 Antibiotic Action." Antimicrobial Agents and Chemotherapy 45, no. 11 (November 1, 2001): 3128–31. http://dx.doi.org/10.1128/aac.45.11.3128-3131.2001.

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Анотація:

ABSTRACT Microcin H47 is a gene-encoded peptide antibiotic produced by a natural Escherichia coli strain isolated in Uruguay. In order to identify cellular components necessary for its antibiotic action, microcin H47-resistant mutants isolated in this work, as well as previously described mutants affected in membrane proteins, were analyzed. These studies indicated that (i) receptor outer membrane proteins for ferric-catechol siderophores would be involved in microcin-specific binding to the cell surface, (ii) the TonB pathway is needed for microcin H47 uptake, and (iii) the presence of the ATP synthase complex is necessary for microcin action. The possibility that this last structure contains the antibiotic target is discussed.

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21

Jasiński, Dariusz. "MICRON3D scanner for special applications." Mechanik, no. 2 (February 2015): 144/17. http://dx.doi.org/10.17814/mechanik.2015.2.39.

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22

Mau, Jens. "GE expandiert." kma - Klinik Management aktuell 13, no. 10 (October 2008): 15. http://dx.doi.org/10.1055/s-0036-1574880.

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23

Carlson, Steve A., Timothy S. Frana, and Ronald W. Griffith. "Antibiotic Resistance in Salmonella enterica Serovar Typhimurium Exposed to Microcin-Producing Escherichia coli." Applied and Environmental Microbiology 67, no. 8 (August 1, 2001): 3763–66. http://dx.doi.org/10.1128/aem.67.8.3763-3766.2001.

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ABSTRACT Microcin 24 is an antimicrobial peptide secreted by uropathogenicEscherichia coli. Secretion of microcin 24 provides an antibacterial defense mechanism for E. coli. In a plasmid-based system using transformed Salmonella enterica, we found that resistance to microcin 24 could be seen in concert with a multiple-antibiotic resistance phenotype. This multidrug-resistant phenotype appeared when Salmonella was exposed to an E. coli strain expressing microcin 24. Therefore, it appears that multidrug-resistant Salmonellacan arise as a result of an insult from other pathogenic bacteria.

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24

Liao, Xiao Hua, Hai Feng Shi, Nan Song, and Xing Xiang Zhang. "Fabrication of Thermochromatic Microencapsulated Phase Change Materials." Advanced Materials Research 332-334 (September 2011): 1856–59. http://dx.doi.org/10.4028/www.scientific.net/amr.332-334.1856.

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Microencapsulated n-octadecane (MicroC18) and doped with thermochromatic powders (TC-MicroC18) were prepared with melamine-formaldehyde (M-F) resin as the wall via in-situ polymerization. The chemical structure and thermal behavior of microcapsules were investigated using fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC). Experimental results show that 63 wt% n-C18 has been incorporated into microcapsules, and the obvious thermochromatic effect of TC-MicroC18 is displayed with temperature changing. The structure-properties of TC-MicroC18 also is discussed in detail from the aspect of molecular structure.

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25

Лавлинский, V. Lavlinskiy, Жвад, and Akhmed Khashim Khalil Zhvad. "Analysis of the functional possibilities CAD MICROCAP on example of the scheme MOS-transistor." Modeling of systems and processes 7, no. 1 (August 8, 2014): 30–37. http://dx.doi.org/10.12737/4952.

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26

Strahsburger, Erwin, Marcelo Baeza, Octavio Monasterio, and Rosalba Lagos. "Cooperative Uptake of Microcin E492 by Receptors FepA, Fiu, and Cir and Inhibition by the Siderophore Enterochelin and Its Dimeric and Trimeric Hydrolysis Products." Antimicrobial Agents and Chemotherapy 49, no. 7 (July 2005): 3083–86. http://dx.doi.org/10.1128/aac.49.7.3083-3086.2005.

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ABSTRACT Microcin E492 uptake by FepA, Fiu, and Cir is cooperative, with FepA being the main receptor. No TonB-mediated interaction with the ferric catecholate receptors is needed for microcin to exert action at the cytoplasmic membrane. Microcin E492 uptake by the receptors is inhibited by the dimer and trimer of dihydroxybenzoylserine.

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27

Jeziorowski, Anne, and David M. Gordon. "Evolution of Microcin V and Colicin Ia Plasmids in Escherichia coli." Journal of Bacteriology 189, no. 19 (July 20, 2007): 7045–52. http://dx.doi.org/10.1128/jb.00243-07.

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ABSTRACT Survey results and genotypic characterization of Escherichia coli strains demonstrate that the bacteriocins colicin Ia and microcin V coassociate in a strain more often than would be expected by chance. When these two bacteriocins co-occur, they are encoded on the same conjugative plasmid. Plasmids encoding colicin Ia and microcin V are nonrandomly distributed with respect to the genomic background of the host strain. Characterization of microcin V and colicin Ia nucleotide variation, together with the backbone of plasmids encoding these bacteriocins, indicates that the association has evolved on multiple occasions and involves the movement of the microcin V operon, together with the genes iroNEDCB and iss, onto a nonrandom subset of colicin Ia plasmids. The fitness advantage conferred on cells encoding both colicin Ia and microcin V has yet to be determined.

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28

Pons, Anne-Marie, Nathalie Zorn, David Vignon, François Delalande, Alain Van Dorsselaer, and Gilles Cottenceau. "Microcin E492 Is an Unmodified Peptide Related in Structure to Colicin V." Antimicrobial Agents and Chemotherapy 46, no. 1 (January 2002): 229–30. http://dx.doi.org/10.1128/aac.46.1.229-230.2002.

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ABSTRACT The pore-forming microcin E492 was purified by solid-phase extraction and reversed-phase high-pressure liquid chromatography. Its molecular mass was 7,886 Da. The entire 84-amino-acid sequence was determined. There is no postranslational modification in the secreted microcin, and the sequence has homologies with the sequence of the microcin colicin V.

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29

Zhao, Zhe, Lauren J. Eberhart, Lisa H. Orfe, Shao-Yeh Lu, Thomas E. Besser, and Douglas R. Call. "Genome-Wide Screening Identifies Six Genes That Are Associated with Susceptibility to Escherichia coli Microcin PDI." Applied and Environmental Microbiology 81, no. 20 (July 24, 2015): 6953–63. http://dx.doi.org/10.1128/aem.01704-15.

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ABSTRACTThe microcin PDI inhibits a diverse group of pathogenicEscherichia colistrains. Coculture of a single-gene knockout library (BW25113;n= 3,985 mutants) against a microcin PDI-producing strain (E. coli25) identified six mutants that were not susceptible (ΔatpA, ΔatpF, ΔdsbA, ΔdsbB, ΔompF, and ΔompR). Complementation of these genes restored susceptibility in all cases, and the loss of susceptibility was confirmed through independent gene knockouts inE. coliO157:H7 Sakai. Heterologous expression ofE. coliompFconferred susceptibility toSalmonella entericaandYersinia enterocoliticastrains that are normally unaffected by microcin PDI. The expression of chimeric OmpF and site-directed mutagenesis revealed that the K47G48N49region within the first extracellular loop ofE. coliOmpF is a putative binding site for microcin PDI. OmpR is a transcriptional regulator forompF, and consequently loss of susceptibility by the ΔompRstrain most likely is related to this function. Deletion of AtpA and AtpF, as well as AtpE and AtpH (missed in the original library screen), resulted in the loss of susceptibility to microcin PDI and the loss of ATP synthase function. Coculture of a susceptible strain in the presence of an ATP synthase inhibitor resulted in a loss of susceptibility, confirming that a functional ATP synthase complex is required for microcin PDI activity. Intransexpression ofompFin the ΔdsbAand ΔdsbBstrains did not restore a susceptible phenotype, indicating that these proteins are probably involved with the formation of disulfide bonds for OmpF or microcin PDI.

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30

Patzer, S. I., M. R. Baquero, D. Bravo, F. Moreno, and K. Hantke. "The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN." Microbiology 149, no. 9 (September 1, 2003): 2557–70. http://dx.doi.org/10.1099/mic.0.26396-0.

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The colicin G producer Escherichia coli CA46, the colicin H producer E. coli CA58 and E. coli Nissle 1917 (DSM 6601) were shown to produce microcin H47 and the newly described microcin M. Both microcins were exported like colicin V by an RND-type export system, including TolC. The gene cluster encoding microcins H47 and M in strains CA46 and CA58 is nearly identical to that in strain DSM 6601, except that two additional genes are included. A Fur box identified in front of the microcin-encoding genes explained the observed iron regulation of microcin production. The catecholate siderophore receptors Fiu, Cir and FepA from E. coli and IroN, Cir and FepA from Salmonella were identified as receptors for microcins M, H47 and E492. IroN takes up the glucose-containing catecholate siderophore salmochelin, whose synthesis is encoded in the iro gene cluster found in Salmonella and certain, often uropathogenic, E. coli strains. A gene in this iro cluster, iroB, which encodes a putative glycosyltransferase, was also found in the microcin H47/M and microcin E492 gene clusters. These microcins could aid the producing strain in competing against enterobacteria that utilize catecholate siderophores.

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31

Herrero, M., R. Kolter, and F. Moreno. "Effects of Microcin B17 on Microcin B17-immune Cells." Microbiology 132, no. 2 (February 1, 1986): 403–10. http://dx.doi.org/10.1099/00221287-132-2-403.

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32

Ichikawa, Akihiko, Ayae Honda, Miho Ejima, Tamio Tanikawa, Fumihito Arai, and Toshio Fukuda. "In-Situ Formation of a Gel Microbead for Laser Micromanipulation of Microorganisms, DNA, and Viruses." Journal of Robotics and Mechatronics 19, no. 5 (October 20, 2007): 569–76. http://dx.doi.org/10.20965/jrm.2007.p0569.

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We proposein situformation of gel microbeads made of a thermoreversible hydrogel for indirect laser micromanipulation of microorganisms, DNA, and viruses. Using a 1064 nm laser, we irradiated an aqueous solution mixed with poly-(N-isopropylacrylamide) through a high- magnification lens, thereby forming a gel microbead through heating at the laser focus. The gel microbead, trapped by the laser, was used to indirectly manipulate micro- and nano-scale samples. Laser tweezers stably handle micro-scale object ranging from several tens of nm to several hundreds of µm. This cannot be done with nano-scale objects of a few nm, however, due to laser beam heating. We demonstrate how to manipulate microorganisms, DNA, and viruses indirectly using a gel microbead made from an aqueous poly-(N-isopropylacrylamide) solution. We reduced laser power for gel microbead formation, and used the gel microbead trapped by the laser to manipulate microorganisms, DNA, and viruses.

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33

Gao, Yu Xin, Jia Yu Xiang, Jun Wang, and Bao Ying Yu. "Study on Mechanical Properties of Microbead Modified Super Sulfate Cement Concrete." Key Engineering Materials 629-630 (October 2014): 455–61. http://dx.doi.org/10.4028/www.scientific.net/kem.629-630.455.

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The microbead modified super sulfate cement (SSC) was employed to prepare C60~C80 concrete, this work emphasis on mechanical properties and micro-mechanism, besides, the service behaviors of fresh concrete was tested. The SSC concrete mechanical properties influenced by particles size of microbead, slag and gypsum were discussed. Mechanisms of mechanical properties changes were elaborated from micro, meso and macro scales, mechanical model was established and relevant rules of mechanical properties development were obtained. Experiment results show that compressive strength increased with the largerer specific surface area of microbead, slag powder and gypsum. There was a promoting effect for SSC concrete hydration reaction by appropriate dosage (10%~20%) of microbead, which had prominent interstitial effect and system with excellent gradation, and fresh concrete workability was significantly improved. The compressive strength—curing ages model of high strength SSC concrete was established by half value intensity index, which coincide well with measured values. Key words: super sulphated cement concrete, microbead, mechanical properties, mechanical model, specific surface area

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34

Roos, Linda L., Steven L. Wise, Michael E. Yoes, and Thomas R. Rocklin. "Conducting Self-Adapted Testing Using Microcat." Educational and Psychological Measurement 56, no. 5 (October 1996): 821–27. http://dx.doi.org/10.1177/0013164496056005008.

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35

Stone, Clement A. "Testing Software Review: MicroCAT Version 3.0." Educational Measurement: Issues and Practice 8, no. 3 (September 1989): 33–38. http://dx.doi.org/10.1111/j.1745-3992.1989.tb00331.x.

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36

Knyazeva, Anzhela. "Financial innovation in microcap public offerings." Journal of Banking & Finance 100 (March 2019): 283–305. http://dx.doi.org/10.1016/j.jbankfin.2018.06.012.

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37

Hu, Pin Pin, Qi Dong Gai, Qing Li, and Xin Tang. "Effect of Microcast-X Fine Grain Casting on the Microstructure and Mechanical Properties of K492M Alloy at 760°C." Materials Science Forum 849 (March 2016): 549–56. http://dx.doi.org/10.4028/www.scientific.net/msf.849.549.

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The effect of Microcast-X fine grain casting on the microstructure and mechnical property K492M alloy at 760°C of was investigated. The results indicated that Microcast-X fine grain casting decreased grain size and dendrite space of γ′ phase and γ/γ′ eutectic. In addition, the element segregation decreased significantly compared to conventional casting technique. Also, the size and distribution of MC carbide were improved. By Microcast-X fine grain casting, the tensile strength increased from 934MPa of conventional casting alloy to 1089MPa and the elongation increased from 1.9% to 5.7%. In addition, the stress-rupture life increased from 28.8h of conventional casting alloy to 72.5h. And the fracture mechanism for the alloys by Microcast-X fine grain casting is trans-granular fracture toughness.

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38

Pomares, María Fernanda, Paula A. Vincent, Ricardo N. Farías, and Raúl A. Salomón. "Protective Action of ppGpp in Microcin J25-Sensitive Strains." Journal of Bacteriology 190, no. 12 (April 11, 2008): 4328–34. http://dx.doi.org/10.1128/jb.00183-08.

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ABSTRACT As Escherichia coli strains enter the stationary phase of growth they become more resistant to the peptide antibiotic microcin J25. It is known that starvation for nutrients such as amino acids or glucose leads to increases in guanosine 3′,5′-bispyrophosphate (ppGpp) levels and that the intracellular concentration of this nucleotide increases as cells enter the stationary phase of growth. Therefore, we examined the effects of artificially manipulating the ppGpp levels on sensitivity to microcin J25. A direct correlation was found between ppGpp accumulation and microcin resistance. Our results indicate that the nucleotide is required to induce production of YojI, a chromosomally encoded efflux pump which, in turn, expels microcin from cells. This would maintain the intracellular level of the antibiotic below a toxic level.

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39

Sablé, S., M. Duarte, D. Bravo, I. Lanneluc, A. M. Pons, G. Cottenceau, and F. Moreno. "Wild-type Escherichia coli producing microcins B17, D93, J25, and L; cloning of genes for microcin L production and immunity." Canadian Journal of Microbiology 49, no. 5 (May 1, 2003): 357–61. http://dx.doi.org/10.1139/w03-047.

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For the first time, an Escherichia coli strain producing four microcins (Mcc), B17, D93, J25, and L, and showing immunity to Mcc V was isolated and characterized. Each of the gene clusters encoding the production of Mcc B17, D93, and L was cloned separately. The gene cluster for Mcc L was cloned within a 13.5-kb HindIIISalI fragment, which includes the Mcc V immunity gene, cvi.Key words: microcin B17, microcin D93, microcin L, multiproduction, cloning.

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40

Socías, Sergio B., Paula A. Vincent, and Raúl A. Salomón. "The Leucine-Responsive Regulatory Protein, Lrp, Modulates Microcin J25 Intrinsic Resistance in Escherichia coli by Regulating Expression of the YojI Microcin Exporter." Journal of Bacteriology 191, no. 4 (December 12, 2008): 1343–48. http://dx.doi.org/10.1128/jb.01074-08.

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ABSTRACT Many Escherichia coli K-12 strains display an intrinsic resistance to the peptide antibiotic microcin J25. In this study, we present results showing that the leucine-responsive regulatory protein, Lrp, is involved in this phenotype by acting as a positive regulator of YojI, a chromosomally encoded efflux pump which expels microcin out of cells. Exogenous leucine antagonizes the effect of Lrp, leading to a diminished expression of the pump and an increased susceptibility to microcin J25.

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41

Rodríguez, Eliana, and Magela Laviña. "The Proton Channel Is the Minimal Structure of ATP Synthase Necessary and Sufficient for Microcin H47 Antibiotic Action." Antimicrobial Agents and Chemotherapy 47, no. 1 (January 2003): 181–87. http://dx.doi.org/10.1128/aac.47.1.181-187.2003.

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ABSTRACT It had been previously determined that the presence of FoF1 ATP synthase was required for microcin H47 antibiotic action. In this work, microcin-resistant atp mutants were genetically analyzed. Their mutations, originated by Tn5 insertion, in all cases were found to affect determinants for the Fo portion of ATP synthase. To discern if microcin action required the presence of the entire complex or if the Fo proton channel would suffice, recombinant plasmids carrying different segments of the atp operon were constructed and introduced into an atp deletion strain. The phenotypic analysis of the strains thus obtained clearly indicated that the presence of the Fo proton channel was absolutely required for microcin H47 action, while the F1 catalytic portion was found to be dispensable. Furthermore, when any of the three components of the proton channel was missing, total resistance to the antibiotic ensued. Complementation analysis between atp::Tn5 chromosomal mutations and recombinant atp plasmid constructions further supported the idea that the proton channel would be the minimal structure of the ATP synthase complex needed for microcin H47 antibiotic action.

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42

Pons, Anne-Marie, François Delalande, Mariela Duarte, Stéphanie Benoit, Isabelle Lanneluc, Sophie Sablé, Alain Van Dorsselaer, and Gilles Cottenceau. "Genetic Analysis and Complete Primary Structure of Microcin L." Antimicrobial Agents and Chemotherapy 48, no. 2 (February 2004): 505–13. http://dx.doi.org/10.1128/aac.48.2.505-513.2004.

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ABSTRACT Escherichia coli LR05, in addition to producing MccB17, J25, and D93, secretes microcin L, a newly discovered microcin that exhibits strong antibacterial activity against related Enterobacteriaceae, including Salmonella enterica serovars Typhimurium and Enteritidis. Microcin L was purified using a two-step procedure including solid-phase extraction and reverse-phase C18 high-performance liquid chromatography. A 4,901-bp region of the DNA plasmid of E. coli LR05 was sequenced revealing that the microcin L cluster consists of four genes, mclC, mclI, mclA, and mclB. The structural gene mclC encoded a 105-amino-acid precursor with a 15-amino-acid N-terminal extension ending with a Gly-Ala motif upstream of the cleavage site. This motif is typical of the class II microcins and other gram-positive bacteriocins exported by ABC transporters. The mclI immunity gene was identified upstream of the mclC gene and encodes a 51-amino-acid protein with two potential transmembrane domains. Located on the reverse strand, two genes, mclA and mclB, encoded the proteins MclA and MclB, respectively. They bear strong relatedness with the ABC transporter proteins and accessory factors involved in the secretion of microcins H47, V, E492, and 24. The microcin L genetic system resembles the genetic organization of MccV. Furthermore the MccL primary structure has been determined. It is a 90-amino-acid peptide of 8,884 Da with two disulfide bridges. The N-terminal region has significant homologies with several gram-positive bacteriocins. The C-terminal 32-amino-acid sequence is 87.5% identical to that of MccV. Together, these results strongly indicate that microcin L is a gram-negative class II microcin.

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43

Said, Nur Azura Mohd, Gregoire Herzog, Karen Twomey, and Vladimir I. Ogurtsov. "Electrochemical Characterization of Silicon-Based Gold Microband Electrode Array and its Application for Labelless T-2/HT-2 Toxin Immunosensing." Materials Science Forum 1055 (March 4, 2022): 137–46. http://dx.doi.org/10.4028/p-3lk2gn.

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Microelectrode arrays (MEAs) are gaining interest in electroanalysis owing to its distinctive voltammetry properties compared to its macro counterparts. Among the MEAs widely fabricated and studied are microdisc array and microband array. We report here the microfabrication of 10 μm microband array (number of band in an array, N=17) and its application in labelless impedimetric sensing of T-2/HT-2 toxin. The microband array has recess depth (i.e. Si3N4 passivation thickness) of 200 nm. Upon fabrication, the device was first characterized via visual inspection and electrochemical analysis. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) studies were performed in 1 mM ferrocenecarboxylic acid (FCA) in 0.01 M PBS, pH 7.4. At scan rate of 100 mv s-1, cyclic voltammogram for the microband array exhibited a slight peak-shaped CV; and was found to be scan-rate dependent. Experimental limiting current of the microband array (529±7 nA) was slightly lower compared to the calculated theoretical current (632 nA) indicating mixed diffusion profile of the microband array. The device was then employed in immunosensor construction for T-2/HT-2 toxins detection. T-2 mycotoxin and its metabolite (HT-2), are target of concern in the biosensing application due to its lethal toxicity and prominent presence in EU grains industry. Surface functionalization for anti-T-2 monoclonal antibody (mAb) immobilization was first achieved via surface hydroxylation with plasma cleaning and piranha solution treatment, followed by (3-Aminopropyl) triethoxysilane (APTES) silanization and 15 minutes pre-incubation with various concentrations of anti-T-2 toxin mAb in EDC/NHS mixture. The optimal concentrations for anti-T-2 toxin mAb immobilization on the microband array surface was determined at 0.75 mg mL-1. Based on the calibration curve developed in buffer solution system, the functionalized microband array was proven sensitive as it was able to detect T-2/HT-2 toxin at low dynamic working range (0-25 ppb) and limit of quantitation (LOQ) achieved at 4.89 ppb.

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44

Wells, William. "Microbead expression arrays." Genome Biology 1 (2000): spotlight—20000606–01. http://dx.doi.org/10.1186/gb-spotlight-20000606-01.

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45

Laviña, M., C. Gaggero, and F. Moreno. "Microcin H47, a chromosome-encoded microcin antibiotic of Escherichia coli." Journal of Bacteriology 172, no. 11 (1990): 6585–88. http://dx.doi.org/10.1128/jb.172.11.6585-6588.1990.

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46

del Castillo, Francisco J., Ignacio del Castillo, and Felipe Moreno. "Construction and Characterization of Mutations at Codon 751 of the Escherichia coli gyrB Gene That Confer Resistance to the Antimicrobial Peptide Microcin B17 and Alter the Activity of DNA Gyrase." Journal of Bacteriology 183, no. 6 (March 15, 2001): 2137–40. http://dx.doi.org/10.1128/jb.183.6.2137-2140.2001.

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ABSTRACT Microcin B17 is a peptide antibiotic that inhibits DNA replication in Escherichia coli by targeting DNA gyrase. Previously, two independently isolated microcin B17-resistant mutants were shown to harbor the same gyrB point mutation that results in the replacement of tryptophan 751 by arginine in the GyrB polypeptide. We used site-directed mutagenesis to construct mutants in which tryptophan 751 was deleted or replaced by other amino acids. These mutants exhibit altered DNA gyrase activity and different levels of resistance to microcin B17.

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47

Benfield, Camilla TO, Farrell MacKenzie, Markus Ritzefeld, Michela Mazzon, Stuart Weston, Edward Tate, Boon Han Teo, et al. "Bat IFITM3 restriction depends on S-palmitoylation and a polymorphic site within the CD225 domain." Life Science Alliance 3, no. 1 (December 11, 2019): e201900542. http://dx.doi.org/10.26508/lsa.201900542.

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Host interferon-induced transmembrane proteins (IFITMs) are broad-spectrum antiviral restriction factors. Of these, IFITM3 potently inhibits viruses that enter cells through acidic endosomes, many of which are zoonotic and emerging viruses with bats (order Chiroptera) as their natural hosts. We previously demonstrated that microbat IFITM3 is antiviral. Here, we show that bat IFITMs are characterized by strong adaptive evolution and identify a highly variable and functionally important site—codon 70—within the conserved CD225 domain of IFITMs. Mutation of this residue in microbat IFITM3 impairs restriction of representatives of four different virus families that enter cells via endosomes. This mutant shows altered subcellular localization and reduced S-palmitoylation, a phenotype copied by mutation of conserved cysteine residues in microbat IFITM3. Furthermore, we show that microbat IFITM3 is S-palmitoylated on cysteine residues C71, C72, and C105, mutation of each cysteine individually impairs virus restriction, and a triple C71A-C72A-C105A mutant loses all restriction activity, concomitant with subcellular re-localization of microbat IFITM3 to Golgi-associated sites. Thus, we propose that S-palmitoylation is critical for Chiropteran IFITM3 function and identify a key molecular determinant of IFITM3 S-palmitoylation.

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48

Portrait, V., S. Gendron-Gaillard, G. Cottenceau, and A. M. Pons. "Inhibition of pathogenicSalmonellaenteritidisgrowth mediated byEscherichia colimicrocin J25 producing strains." Canadian Journal of Microbiology 45, no. 12 (December 1, 1999): 988–94. http://dx.doi.org/10.1139/w99-106.

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For the first time, microcin-producing strains showing inhibitory activities against enteropathogen Salmonella enteritidis were isolated from poultry intestinal contents. Among the numerous strains isolated, two strains of Escherichia coli, named J02 and J03, showing the greatest activities against S. enteritidis, were studied. Biochemical tests and purification identified the main antagonist compound produced as microcin J25. In order to evaluate the protective potential of E. coli J02 and J03 against S. enteritidis infection, the ability of these strains to inhibit growth of S. enteritidis was investigated in mixed culture. A strong antagonist activity was obtained with a preculture phase of the active strain in minimal medium before incubation with S. enteritidis. In a bioreactor experiment simulating the chicken gastric and intestinal tract environment, a mixture of the two strains E. coli J02 and J03, provided an enhanced inhibitory effect. Microcinogenic strain activities were not affected by bile, pancreatic enzymes addition, or acidic conditions. These results suggest the relevant role of microcin-producing microorganisms in microbial intestinal ecology. To conclude, this study shows that microcin J25 strains could exert a beneficial protective effect against S. enteritidis growth in situ.Key words: microcin J25, Salmonella, mixed cultures.

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49

Pazoki, M., J. Oscarsson, L. Yang, B. W. Park, E. M. J. Johansson, H. Rensmo, A. Hagfeldt, and G. Boschloo. "Mesoporous TiO2 microbead electrodes for solid state dye-sensitized solar cells." RSC Adv. 4, no. 91 (2014): 50295–300. http://dx.doi.org/10.1039/c4ra10049b.

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Mesoporous TiO₂ microbead films have been investigated as working electrode for solid state dye sensitized solar cells and 3.5% efficiency was achieved. Low trap density of microbead film leads to high voltage and fast electron transport.

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50

Valenti, Michael. "The Proper Coat of Oil." Mechanical Engineering 122, no. 01 (January 1, 2000): 62–64. http://dx.doi.org/10.1115/1.2000-jan-6.

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This article illustrates that controlling the lubricant spray keeps presses running cleaner and longer in die stamping shops. The MicroCoat system enabled Warwick, Rhode Island-based ETCO Inc.’s Engineered Products division to minimize oil mist in its press room, reduce maintenance downtime, improve the quality of finished parts, and increase the throughput of it. After installing MicroCoat on all 10 of its Bruderer presses, ETCO has increased the number of hits between sharpenings by 50 percent, or 500,000 strokes per press with much less tool damage. The 2.5-inch-high MicroCoat spray valves use low-volume, low-pressure air to apply die stamping lubricant in fine, even films without producing waste, mist, or overspray. The transparent reservoir of the MicroCoat System feeds metered amounts of lubricant to the compact spray valves mounted inside this Bruderer die press between the metal feed stock and the tool.

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