Добірка наукової літератури з теми "Metal-Chelating Peptide"
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Статті в журналах з теми "Metal-Chelating Peptide":
Matsubara, Teruhiko, Yuko Hiura, and Katsuhiro Kawashiro. "Biocombinatorial Selection of Metal Ion-Chelating Peptides." International Journal of Modern Physics B 17, no. 08n09 (April 10, 2003): 1324–28. http://dx.doi.org/10.1142/s0217979203018946.
Kani, Hatice K., Ebru K. Kocazorbaz, and Figen Zihnioglu. "Investigation and isolation of peptide based antiglycating agents from various sources." Turkish Journal of Biochemistry 44, no. 5 (October 25, 2019): 699–705. http://dx.doi.org/10.1515/tjb-2018-0294.
Chan, Pei-Teng, Patricia Matanjun, Cahyo Budiman, Rossita Shapawi, and Jau-Shya Lee. "Novel Peptide Sequences with ACE-Inhibitory and Antioxidant Activities Derived from the Heads and Bones of Hybrid Groupers (Epinephelus lanceolatus × Epinephelus fuscoguttatus)." Foods 11, no. 24 (December 9, 2022): 3991. http://dx.doi.org/10.3390/foods11243991.
Smith, M. C., T. C. Furman, J. A. Cook, T. Ingolia, and H. Hsiung. "Chelating peptide-immobilized metal ion affinity chromatography." Journal of Inorganic Biochemistry 36, no. 3-4 (August 1989): 277. http://dx.doi.org/10.1016/0162-0134(89)84385-5.
Daubit, Isabelle Marie, and Nils Metzler-Nolte. "On the interaction of N-heterocyclic carbene Ir+I complexes with His and Cys containing peptides." Dalton Transactions 48, no. 36 (2019): 13662–73. http://dx.doi.org/10.1039/c9dt01338e.
Alies, Bruno, Jacob D. Wiener, and Katherine J. Franz. "A prochelator peptide designed to use heterometallic cooperativity to enhance metal ion affinity." Chemical Science 6, no. 6 (2015): 3606–10. http://dx.doi.org/10.1039/c5sc00602c.
Irankunda, Rachel, Jairo Andrés Camaño Echavarría, Cédric Paris, Loïc Stefan, Stéphane Desobry, Katalin Selmeczi, Laurence Muhr, and Laetitia Canabady-Rochelle. "Metal-Chelating Peptides Separation Using Immobilized Metal Ion Affinity Chromatography: Experimental Methodology and Simulation." Separations 9, no. 11 (November 14, 2022): 370. http://dx.doi.org/10.3390/separations9110370.
Luisi, Grazia, Azzurra Stefanucci, Gokhan Zengin, Marilisa Dimmito, and Adriano Mollica. "Anti-Oxidant and Tyrosinase Inhibitory In Vitro Activity of Amino Acids and Small Peptides: New Hints for the Multifaceted Treatment of Neurologic and Metabolic Disfunctions." Antioxidants 8, no. 1 (December 26, 2018): 7. http://dx.doi.org/10.3390/antiox8010007.
Lupaescu, Ancuta-Veronica, Ion Sandu, Brindusa Alina Petre, Laura Ion, Catalina-Ionica Ciobanu, and Gabi Drochioiu. "NAP Neuroprotective Peptide and its Analogs: Simultaneously Copper and Iron Binding and Reduction." Revista de Chimie 70, no. 5 (June 15, 2019): 1784–90. http://dx.doi.org/10.37358/rc.19.5.7215.
Dayob, Kenana, Aygul Zengin, Ruslan Garifullin, Mustafa O. Guler, Timur I. Abdullin, Abdulla Yergeshov, Diana V. Salakhieva, Hong Hanh Cong, and Mohamed Zoughaib. "Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell Behavior." Micromachines 14, no. 4 (April 19, 2023): 883. http://dx.doi.org/10.3390/mi14040883.
Дисертації з теми "Metal-Chelating Peptide":
El, Hajj Sarah. "Methodologies for Screening Metal-Chelating Peptides in Protein Hydrolysates for their Antioxidant Properties." Electronic Thesis or Diss., Université de Lorraine, 2022. http://www.theses.fr/2022LORR0031.
Currently, the discovery of new biomolecules is essential for various industrial applications. Peptides produced by hydrolysis of plant or animal proteins are of interest because of their potential bioactivities. Some peptides, by their ability to complex iron(II), can inhibit and slow down oxidation phenomena. To date, the discovery of new bioactive molecules is generally based on long and fastidious empirical approaches, involving large quantities of solvents, harmful to the environment. Therefore, it is important to develop new and highly sensitive screening methods before implementing a selective separation process of these peptides of interest present in mixture. The objective of this thesis is therefore to (i) develop highly sensitive screening methods to identify the presence of these metal chelating peptides (MCPs) in hydrolysates, produced in the laboratory, before undertaking a time-consuming separation phase and (2) to study their antioxidant activities, using biochemical and cellular assays. In the first approach, time-resolved molecular dynamics (switchSENSE) was first implemented on model peptides capable of complexing metals and then applied to non-filtrated soy and tilapia protein hydrolysates. It has proven to be very sensitive for detecting the presence of MCPs in peptide hydrolysates. This technology is based on the electrical movement of DNA strands, present on the surface of gold microelectrodes, on which are immobilized metal ions. When a peptide has an affinity for an immobilized metal ion and thus forms a peptide-metal complex, this results in a variation of the measured fluorescence signal. On the other hand, MCPs were screened in ultrafiltrated soy and pea protein hydrolysates using Surface Plasmon Resonance (SPR). This advanced technique, allows to determine an affinity constant between a peptide and a metal ion immobilized on a microchip at the molecular scale. It is used here in an original way to study different hydrolysates and thus screen the best hydrolysis condition to produce MCPs. The biological interest in exploring metal-chelating activity in these hydrolysates is to evaluate their antioxidant power in cellulo. These peptides could be promising for ferroptosis inhibition by chelating excess iron ions and thus contribute to the prevention of cell death in several diseases including Atherosclerosis (AS). We aimed to develop a ferroptosis model (induction and rescuing) on human aortic smooth muscle cells to mimic ferroptosis happening during AS development. The ferroptosis model was developed in three steps taking into consideration three essential parameters such as the concentration of ferroptosis inducers (Erastin and ferric ions), the time and the medium of incubation. At each step, 2 biomarkers i.e. intracellular concentration of GSH and lipid peroxidation were followed by NDA and TBARS methods, respectively. The rescue of the ferroptosis model was validated using metal chelators such as DFO and DFr, which are also control molecule regarding MCP activity
Irankunda, Rachel. "Nickel Chelating Peptides & Chromatography : From Peptides Separation Simulation up to their Antioxidant Activities - related Applications." Electronic Thesis or Diss., Université de Lorraine, 2023. http://www.theses.fr/2023LORR0213.
Metal-Chelating Peptides (MCPs), from protein hydrolysates, present various applications in nutrition, pharmacy, cosmetic etc. Yet, the empirical approach generally used to discover bioactive peptides from hydrolysates is time consuming and expensive due to many steps of fractionation, separation and biological activities evaluation. Thus, this PhD aimed to develop a novel approach for MCPs separation prediction using chromatography modelling and simulation based on the analogy between Immobilized Metal ion Affinity Chromatography (IMAC) and Surface Plasmon Resonance (SPR). For the first time, the SPR-IMAC analogy was experimentally investigated on 22 peptides and 70% of them validated this analogy, since peptides well retained in IMAC were also endowed with a good affinity for Ni2+ in SPR. In the second time, peptides with high affinity for Ni2+ (i.e low dissociation constant KD in SPR and a high retention time in IMAC) were used to study the modelling and simulation of peptide concentration profiles at the column outlet in IMAC. Since knowledge of adsorption isotherms was required to perform simulation, it was necessary to develop a methodology for predicting Langmuir isotherm parameters in IMAC from SPR data. The validity of simulation was evaluated by comparing experimental and simulated retention times that should be close for reliable prediction. Therefore, several approaches were evaluated to determine Langmuir sorption parameters, the most interesting one introduces a correction factor on the maximum adsorption capacity qmax alone, assuming that the affinity of peptides for immobilized Ni2+ did not change depending on the technology used (SPR vs. IMAC), thus affinity constant KA was not modified. Meanwhile, industrial application of MCPs and hydrolysates were studied. First, pea protein hydrolysates were produced by either Alcalase® followed by Flavourzyme® (Alc+Flav≤1kDa) or Protamex® followed by Flavourzyme® (Prot+Flav≤1kDa). SwitchSENSE® technology evidences the presence of Ni2+ chelating peptides and antioxidants tests showed that Prot+Flav≤1kDa has higher radical scavenging and reducing power, related to its higher degree of hydrolysis and small-size peptides quantity. Secondly, pea hydrolysates and MCPs were investigated for their ability to inhibit the lipid oxidation in emulsions. They slowed down lipid oxidation through chelation of prooxidant (metals such as Fe2+) reducing primary and secondary oxidation products responsible of deterioration of lipid containing products. Thus, pea hydrolysates and MCPs could be used as antioxidants in food and cosmetic products, as alternative to chemicals such as EDTA, BHT and TBHQ
Paris, Cédric. "Développement de nouvelles approches analytiques pour le criblage de peptides chélateurs de fer." Electronic Thesis or Diss., Université de Lorraine, 2021. http://www.theses.fr/2021LORR0088.
Faced with the growing need for new bioactive compounds of natural origin, by-products from the agro-food industry and the processing of agro-resources constitute a strategic resource to be exploited. In fact, the enzymatic hydrolysis of plant or animal proteins makes it possible to generate a wide variety of peptide sequences with potential biological properties: antihypertensive, antithrombotic, anticancer, opioid, antimicrobial. Despite the bioactive potential of certain peptides, their uncertain presence and their low concentration in a protein hydrolysate (a complex mixture sometimes made up of more than a hundred peptides) limit their purification and use. Also, bioactive peptides could be screened before their purification in order to initiate the separation step only if activity is proven. Antioxidant power is a generic term which groups together various chemical mechanisms such as anti-free radical activity, inhibition of lipid peroxidation, or even metal chelation. By chelating the transition metals naturally present in vivo (iron, copper), the chelating peptides could be used as indirect antioxidants and thus act against oxidative stress. The main objective of this PhD thesis is to develop original methods for high throughput screening of iron-chelating peptides present in protein hydrolysates. Ultimately, these methods could be applied to all types of complex peptide mixtures. The first approach is based on immobilized metal affinity chromatography (IMAC). IMAC is a reference technique for purifying metal-chelating peptides in hydrolysates. Thanks to the specificity of interaction between a given metal – immobilized on the stationary phase IMAC – and determined complexing groups, it is possible to selectively identify the chelators present in complex mixtures. Our objective being to achieve a rapid detection of these molecules of interest, we carried out an on-line coupling with mass spectrometry (MS). The second strategy consists of evaluating the formation of iron-peptide complexes in solution. In this case, all the electron acceptor sites of the metal are accessible (unlike the IMAC technique which presents a potential bias from this point of view) and, on the other hand, the solubilization conditions can simulate the target medium (i.e. the intracellular medium). In addition, the observation of the peptidic form complexed with iron (FeII or FeIII) provides direct and irrefutable proof of the chelating capacity of a peptide. Thus, the identification of a chelating peptide can be carried out by the concomitant detection of its free form (peptide) and of its complexed form (iron-peptide). In this approach, mass spectrometry – thanks to its sensitivity and its specificity - is a technique of choice for carrying out the desired screening. After having been tested on synthetic peptides (pure solutions and mixture), the two protocols were applied to a real protein hydrolysate. The preliminary results are promising and make it possible to envisage, in the short term, the automated screening of various real hydrolysates for the search for iron(II)- and iron(III)-chelating peptides
陳愷悌. "Preparation, Purification, and Immobilization of Schistosoma Japonicum Glutathions-S-transferase Tagged with a Metal Chelating Peptide." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/46328224893948298568.
國立臺灣科技大學
化學工程技術研究所
86
Application of a metal affinity tag constituted of six histidines, 6xHis tag, in immobilized metal affinity chromatography (IMAC) could simplify and integrate the purification and immobilization of recombinant protein together. We have engineered the 6xHis tag to the C-terminus of Schistosoma japonicum glutathione-S-transferase (GST) and created a fustion protein GST/His. The production, activtity, purity, proteolysis, and metal affinity of GST/His were extensively studied, as well as solid state refolding of GST/His The results showed that: (1) GST/His expression using using T7 promoter was better produced than GST expression using tac promoter. (2)The 6xHis tag had no effect on the activity and immunity of GST. (3) The optimization condition to purify GST/His with Ni2+-NTA gel was to include 20 mM imidazole in native washing buffer and 10 mM imidazole in denatruing washing buffer. Beause TALON get had better specificity to GST/His than to GST, no imidazole was required in either washing buffer. (4)The optimal immobilization concentration of GST/His on Ni2+-NTA gel was 5~50 駪/ml, resulting the best specific activity. (5)After storage at either 4℃ or 25℃ for one month, the specific activity of immobilized GST/His did not decay. However, the specific activity of immobilized GST/His decayed 60% after 50 days storage at 25℃. (6)We could recove and purify GST/His from inclusion bodies by solid state refolding procedure, where remove of high concentration of denaturing agent was performed stepwise with GST/His immobilized on Ni2+-NTA gel. (7)The proteolysis of the free form of GST/His began from the C-terminus, while that of immobilized GST/His began from the N-terminus.
顏碧秀. "The Use of Atomic Adsorption Spectrometry for the Determination of Metal Nanoparticles and the Interaction of Metal Ions with Chelating Peptides." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/44868514629154878842.
國立中正大學
化學研究所
89
The first part of the thesis is elemental analysis of gold nanoparticles by graphite atomic absorption spectrometry. The aim is to establish the calibration curves of aqueous standards and to measure the gold nanoparticles in nonaqueous sample. The search of optimized conditions for gold nanoparticles is performed first. The use of palladium and magnesium nitrate as a matrix modifier can increase the char and atomization temperature and reduce the matrix interference. This leads to an increase in sensitivity of the determination. The surfaces of ashed gold nanoparticles were observed by atomic force microscopy to examine the ash temperature effect on the gold nanoparticles. The result indicates that the size of the gold nanoparticles do not effect the analysis. Slurry sampling technique is used for gold nanoparticle immobilized to silica. The accuracy of the determinations were confirmed by standard addition method. The sensitivity and detection limit were 30.0pg and 1.5ppb, respectively. It corresponds to the number of 2.7×105 gold particles. The RSD of the determination is poor. This is due to inhomogeneous distribution of gold particles on silica. The 2nd part of this thesis presents the preliminary results of metal ions chelation with peptides by batch and column method. The primary focus is on the selectivity, capacity and mechanism for different metal ionschelation with peptides. Copper bounds strongly to peptide3 (lysine-cysteine-βamino acid)and peptide4(glycine-cysteine- histidine) with the increase of pH value. The capacity of copper to peptide3 was 3.01mg/g by the batch method and 2.42 mg/g by the column method. For peptide3, nickel breaks through the column faster than copper and silver due to the low affinity. The adsorption mechanism is contributed to NH2 and CO-N-R for peptide3, and by NH2 , CO-N-R,and COO-for peptide4 at pH=5.5. The advantages of using the column method are better accuracy for the evalution of loading capacity and the potential for the prediction of adsorption mechanism.
Книги з теми "Metal-Chelating Peptide":
Arya, Rajiv. DEsign and Synthesis of a metal chelating peptide. Ottawa: National Library of Canada, 1995.
Частини книг з теми "Metal-Chelating Peptide":
Sheldon, K. M., R. Arya, and J. Gariépy. "Novel radioimmunoconjugates containing a bifunctional metal chelating peptide." In Peptides, 852–53. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0683-2_285.
Smith, Michele C., James A. Cook, Thomas C. Furman, Paul D. Gesellchen, Dennis P. Smith, and Hansen Hsiung. "Chelating Peptide-Immobilized Metal-Ion Affinity Chromatography." In ACS Symposium Series, 168–80. Washington, DC: American Chemical Society, 1990. http://dx.doi.org/10.1021/bk-1990-0427.ch012.
Sanità Di Toppi, L., M. N. V. Prasad, and S. Ottonello. "Metal Chelating Peptides and Proteins in Plants." In Physiology and Biochemistry of Metal Toxicity and Tolerance in Plants, 59–93. Dordrecht: Springer Netherlands, 2002. http://dx.doi.org/10.1007/978-94-017-2660-3_3.
Fournié-Zaluski, M. C., C. Z. Dong, Y. S. Yang, N. Jullian, and B. P. Roques. "Synthesis, conformational analysis by 1H NMR spectroscopy and metal chelating properties of a cyclic zinc finger derived from the nucleocapsid NCp7 of the human retrovirus HIV-1." In Peptides 1994, 535–36. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-1468-4_242.
Тези доповідей конференцій з теми "Metal-Chelating Peptide":
Bjørlie, Mads, Rachel Irankunda, Jean-Michel Girardet, Sandrine Boschi-Müller, Betül Yesiltas, Charlotte Jacobsen, and Laetitia Canabady-Rochelle. "Screening of Metal-chelating Peptides and Hydrolysates Using Surface Plasmon Resonance and Switchsense." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/zszk2778.
Kaugarenia, Nastassia, Sophie Beaubier, Erwann Durand, François Lesage, Xavier Framboisier, Arnaud Aymes, Pierre Villeneuve, and Romain Kapel. "Optimization of Potent Mineral Chelating Peptides Production from Rapeseed Meal Proteins Proteolysis and Peptide Characterizations." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/ougk6662.
Durand, Erwann, Nastassia Kaugarenia, Nathalie Barouh, Pierre Villeneuve, and Romain Kapel. "Antioxidant chelating peptides production from Rapeseed meal proteins proteolysis." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/whcd7145.
Jacobsen, Charlotte, Ann-Dorit Moltke Sorensen, and Dimitra Marinou. "Enzymatic production of antioxidative and antimicrobial hydrolysates from cod solid side-streams." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/qmqf3129.
Yesiltas, Betul, Charlotte Jacobsen, Egon B. Hansen, Michael Overgaard, Paolo Marcatili, Pedro Garcia-Moreno, Rasmus K. Mikkelsen, and Simon Gregersen. "Physical and oxidative stability of emulsions stabilized with fractionated potato protein hydrolysates obtained from starch production byproduct: Use of bioinformatics and proteomics." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/xxty9713.