Дисертації з теми "Membrane proteins and peptides"
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Käll, Lukas. "Predicting transmembrane topology and signal peptides with hidden Markov models /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-719-7/.
Повний текст джерелаOldham, Alexis Jean. "Modulation of lipid domain formation in mixed model systems by proteins and peptides." View electronic thesis, 2008. http://dl.uncw.edu/etd/2008-1/r1/oldhama/alexisoldham.pdf.
Повний текст джерелаYin, Daniel. "Biophysical investigations into membrane-active peptides and proteins." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:25401447-e37b-4c07-a22d-29718958ac48.
Повний текст джерелаMitchell, Stephen Anthony. "Membrane translocating peptides for the delivery of proteins." Thesis, University of Bath, 2003. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.397763.
Повний текст джерелаOglęcka, Kamila. "Biophysical studies of membrane interacting peptides derived from viral and Prion proteins." Doctoral thesis, Stockholm University, Department of Biochemistry and Biophysics, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-7109.
Повний текст джерелаThis thesis focuses on peptides derived from the Prion, Doppel and Influenza haemagglutinin proteins in the context of bilayer interactions with model membranes and live cells. The studies involve spectroscopic techniques like fluorescence, fluorescence correlation spectroscopy (FCS), circular and linear dichroism (CD and LD), confocal fluorescence microscopy and NMR.
The peptides derived from the Prion and Doppel proteins combined with their subsequent nuclear localization-like sequences, makes them resemble cell-penetrating peptides (CPPs). mPrPp(1-28), corresponding to the first 28 amino acids of the mouse PrP, was shown to translocate across cell membranes, concomitantly causing cell toxicity. Its bovine counterpart bPrPp(1-30) was demonstrated to enter live cells, with and without cargo, mainly via macropinocytosis. The mPrPp(23-50) peptide sequence overlaps with mPrPp(1-28) sharing the KKRPKP sequence believed to encompass the driving force behind translocation. mPrPp(23-50) was however found unable to cross over cell membranes and had virtually no perturbing effects on membranes.
mDplp(1-30), corresponding of the first 30 N-terminal amino acids of the Doppel protein, was demonstrated to be almost as membrane perturbing as melittin. NMR experiments in bicelles implied a transmembrane configuration of its alpha-helix, which was corroborated by LD in vesicle bilayers. The positioning of the induced alpha-helix in transportan was found to be more parallel to the bilayer surface in the same model system.
Positioning of the native Influenza derived fusion peptide in bilayers showed no pH dependence. The glutamic acid enriched variant however, changed its insertion angle from 70 deg to a magic angle alignment relative the membrane normal upon a pH drop from 7.4 to 5.0. Concomitantly, the alpha-helical content dramatically rose from 18% to 52% in partly anionic membranes, while the native peptide’s helicity increased only from 39% to 44% in the same conditions.
Polozov, Ivan V. "Interactions of class A and class L amphipathic helical peptides with model membranes." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0006/NQ30110.pdf.
Повний текст джерелаRedeby, Theres. "Improved Techniques for Protein Analysis Focusing on Membrane Proteins and Hydrophobic Peptides." Doctoral thesis, Stockholm, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-617.
Повний текст джерелаMcKinley, Laura Ellen. "Neutron reflectivity studies of bacterial membranes, peptides and proteins." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28874.
Повний текст джерелаWhiles-Lillig, Jennifer A. "Bicelles : a new system for studying membrane associated peptides and proteins /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2001. http://wwwlib.umi.com/cr/ucsd/fullcit?p3022189.
Повний текст джерелаTrifunovski, Alexandra. "On nogo signaling regulation /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-906-8/.
Повний текст джерелаZheng, Hong. "Designing Peptides to Target Membrane Lipids and to Evaluate Fluorination of Proteins." Thesis, Boston College, 2012. http://hdl.handle.net/2345/3682.
Повний текст джерелаMy graduate research has used engineered peptides to perturb the non-covalent interactions in protein folding, protein-protein association and protein-membrane association. We have focused on understanding the fundamental principles of molecular recognition behind protein-protein and protein-membrane interactions, and further using these principles in protein engineering. This thesis includes three projects. I) Towards Small Molecule Receptors for Membrane Lipids: A Case Study on Phosphatidylserine The lipid composition and distribution of cell membranes play important roles in regulating the physiology of the cell. The lipid composition of plasma membranes is one characteristic feature that can be used to identify cell types and functions. Molecules that specifically recognize a particular lipid are useful as imaging probes for targeting cells or tissues of interest. Protein based lipid binding probes have intrinsic limitations due to their large size and poor pharmacokinetic properties such as slow clearance rate and poor in vivo stability. A plausible strategy to achieve a probe with small size and high binding affinity and selectivity is to use a peptide to mimic the protein lipid-binding domains. As a case study, a cyclic peptide that specifically targets phosphatidylserine containing membranes has been developed. This cyclic peptide is potentially capable of imaging apoptosis in vivo, and the strategy of developing this cyclic peptide can be generalized to the design of peptide-based probes for other lipid species. My research has pointed out a challenging but feasible way to design a peptide that achieves specificity and affinity similar to lipid-binding proteins. (II) Study of Apoptotic Cell Membrane (ACM) Permeant Molecules Noninvasive imaging of apoptosis is highly desirable for the diagnosis of a variety of diseases, as well as for the early prognosis of anticancer treatments. One characteristic feature of apoptotic cells that has been targeted for developing specific biomarkers is enhanced membrane permeability compared to that of healthy cells. Several unrelated molecules that are capable of selectively penetrating the apoptotic cell membrane (ACM) have recently been reported. However, the origin of the altered ACM permeability is poorly understood, as is the scope of molecular structures that can permeate through the ACM. Herein, we report a systematic investigation on the altered ACM permeability. Our results show that simple modifications of commonly used dyes (e.g. fluorescein) afford specific entry into cells at the early stages of apoptosis. The ACM appears to be permeable to molecules of various functional groups and charge, but does discriminate against molecules of large size. The new findings reported here greatly expand the pool of small molecules for imaging cell death, thus facilitating the development of noninvasive imaging agents for apoptosis. (III) Study of Aromatic-Fluorinated Aromatic Interactions in Peptide Systems Therapeutic proteins have been through a remarkable expansion in the last two decades. A general problem that they are facing is poor stability. Protein engineering focuses on solving this problem by incorporating unnatural amino acids into protein sequences to purposefully modify protein structures. Fluorinated aliphatic amino acids have been demonstrated to be effective in stabilizing protein structures and functioning as recognition motifs. In contrast, fluorinated aromatic amino acids are less studied. We investigated the effect of perturbation of fluorination on aromatic residues on the stability of protein model systems, as well as the influence on protein-protein association behavior. The results of this study provided a fundamental understanding of aromatic interactions in protein systems, and guidelines for protein engineering with fluorinated aromatics for stabilizing protein structures or directing specific protein-protein interactions
Thesis (PhD) — Boston College, 2012
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Chemistry
Oglęcka, Kamila. "Biophysical studies of membrane interacting peptides derived from viral and Prion proteins /." Stockholm : Department of Biochemistry and Biophysics, Stockholm University, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-7109.
Повний текст джерелаLeng, Ying. "Neuron-ligand pathfinding on surfaces modified by laminin and laminin-derived peptides." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 78 p, 2006. http://proquest.umi.com/pqdweb?did=1203562381&sid=8&Fmt=2&clientId=8331&RQT=309&VName=PQD.
Повний текст джерелаRigler, Per. "Investigation of peptides and membrane proteins at interfaces using Fourier transform infrared spectroscopy /." Lausanne, 2002. http://library.epfl.ch/theses/?nr=2544.
Повний текст джерела"The results have been partially published in Sevin-Landais, A. [et al.] (2000) Functional immobilisation of the nicotinic acetylcholine receptor in tethered lipid membranes (Biophys Chem 85, 141-152)"
Sengupta, Durba. "Insights into the energetics of membrane-bound peptides towards an understanding of the structural organisation of membrane proteins /." [S.l. : s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=976744465.
Повний текст джерелаGhimire, Harishchandra. "Structure, Dynamics, and Distance Measurements in Membrane Proteins and Peptides using EPR Spectroscopic Techniques." Miami University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=miami1291739688.
Повний текст джерелаKaye, Elena Cortizas. "The Function of Outer Membrane Protein A (OmpA) in Yersinia pestis." Scholarly Repository, 2010. http://scholarlyrepository.miami.edu/oa_theses/58.
Повний текст джерелаRadzey, Hanna Agnes. "Synthesis of fluorescent toxin and nucleotide derivatives to specifically address membrane proteins." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://hdl.handle.net/11858/00-1735-0000-0022-6055-5.
Повний текст джерелаLouie, Sarah. "Wnt signaling regulated by Frizzled and HIPK1 /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/6267.
Повний текст джерелаIsmail, Vian Sdiq Ismail. "Probing lipidation of membrane active peptides and integral membrane proteins by liquid chromatography-mass spectrometry and ion mobility separation-mass spectrometry." Thesis, Durham University, 2017. http://etheses.dur.ac.uk/12424/.
Повний текст джерелаTakahashi, Satoe. "Plasma Membrane Localization of Signaling Proteins in Yeast: a Dissertation." eScholarship@UMMS, 2008. https://escholarship.umassmed.edu/gsbs_diss/364.
Повний текст джерелаBajaj, Vikram Singh. "Dynamic nuclear polarization in biomolecular solid state NMR : methods and applications in peptides and membrane proteins." Thesis, Massachusetts Institute of Technology, 2007. http://hdl.handle.net/1721.1/40874.
Повний текст джерелаIncludes bibliographical references.
Solid state NMR can probe structure and dynamics on length scales from the atomic to the supramolecular. However, low sensitivity limits its application in macromolecules. NMR sensitivity can be improved by dynamic nuclear polarization (DNP), in which electron polarization is transferred to nuclei. We present applications of magic angle spinning NMR that demonstrate its utility for the determination of structure at atomic resolution. We then present new techniques and instrumentation for DNP that permit these methods to be applied to larger systems such as membrane proteins. These applications rest on several advances in instrumentation: millimeter-wave sources and conduits of power to the sample; low-temperature MAS probes incorporating millimeter-wave transmission; cryogenics and pneumatic control systems. We describe a 380 MHz DNP spectrometer incorporating a 250 GHz gyrotron oscillator and present the theory and operation of a 460 GHz gyrotron at the second harmonic of electron cyclotron resonance. We have applied DNP to study trapped photo cycle intermediates of the archael membrane protein bacteriorhodopsin, a light-driven transmembrane ion pump.
(cont.) We have observed the K photointermediate for the first time by NMR and found unexpected conformational heterogeneity in the L intermediate. With multidimensional correlation spectroscopy, we have assigned active site resonances in conformational mixtures of photointermediates of [U-13C,'SN]-bR with high sensitivity. By using non-linear sampling of indirect dimensions, we have observed transient product of K accumulation. We present frequency-selective experiments for amino acid-selective assignments and the measurement of heteronuclear distances and torsion angles in [U-13C, N]-bR and discuss the relevance of these results to its photocycle. In addition, we describe several applications of solid state NMR, including a study of dynamic and structural phase transitions in peptides and proteins near the canonical glass transition temperature. We present resonance width experiments that can be used to measure homonuclear and heteronuclear dipolar couplings in uniformly labeled solids.
(cont.) Finally, we discuss applications to amyloid fibrils, which are protein aggregates that are implicated in diseases of protein misfolding. We report the atomic resolution structure of the disease-associated L 111M mutant of TTR105-115 in an amyloid fibril, and information about the supramolecular structure of fibrils from WT TTRos05115.
by Vikram Singh Bajaj.
Ph.D.
Vermeer, Louic Sebichniev. "NMR struture determination and MD simultations of membrane peptides and proteins : a peptide derived from H+-V-ATPase subunit alpha, and MscL." Toulouse 3, 2009. http://thesesups.ups-tlse.fr/492/.
Повний текст джерелаThe structural properties of a peptide derived from the proton translocating H+-V-ATPase subunit a were studied. An NMR structure was obtained in SDS micelles. Circular dichroism measurements indicated that the peptide formed a beta-sheet at high pH in octylglucoside, while it was 60% alpha-helical in SDS. These findings were explained using molecular dynamics simulations, which indicated that at high pH the peptide took a transmembrane position in SDS but was located in the interface region in octylglucoside. The combination of the NMR structure and the MD simulations allowed us to identify the residues that line the lumenal proton channel
Follit, John A. "Building the Cell's Antenna: Protein Targeting to the Ciliary Membrane: A Dissertation." eScholarship@UMMS, 2012. https://escholarship.umassmed.edu/gsbs_diss/594.
Повний текст джерелаJiang, Ying. "Transfer of the Ribosome-Nascent Chain Complex to the Translocon in Cotranslational Translocation: A Thesis." eScholarship@UMMS, 2007. https://escholarship.umassmed.edu/gsbs_diss/332.
Повний текст джерелаStone-Hulslander, Judith. "Mechanisms of Newcastle Disease Virus-Mediated Membrane Fusion: A Dissertation." eScholarship@UMMS, 1999. https://escholarship.umassmed.edu/gsbs_diss/131.
Повний текст джерелаAdhikari, Anirban. "Regulation of guanine nucelotide exchange in inhibitory G protein alpha subunit by activator of G protein signaling 3 and novel regulatory peptides." Embargoed access until after 12/19/2006, 2005. http://www4.utsouthwestern.edu/library/ETD/etdDetails.cfm?etdID=114.
Повний текст джерелаWeiß, Kerstin [Verfasser], Jörg [Akademischer Betreuer] Enderlein, and Sarah [Akademischer Betreuer] Köster. "Quantifying the diffusion of membrane proteins and peptides / Kerstin Weiß. Gutachter: Jörg Enderlein ; Sarah Köster. Betreuer: Jörg Enderlein." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2013. http://d-nb.info/1044739401/34.
Повний текст джерелаYe, Weihua. "One key to two doors : Dual targeting peptides and membrane mimetics." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-116817.
Повний текст джерелаAt the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 2: In press.
Mitsopoulos, Konstantinos. "The assembly of type III membrane proteins in Escherichia coli." Thesis, University of Sussex, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310262.
Повний текст джерелаMalmberg, Jennie. "The neuroanatomical expression profile of novel membrane proteins. : The effect of macronutrients on gene expression." Thesis, Mälardalen University, School of Sustainable Development of Society and Technology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-1105.
Повний текст джерелаWorldwide obesity is an increasing problem. Apart from the fact that obesity greatly impairs the health, quality and length of life for the affected individuals, it is also has the potential to become a major socioeconomic problem in a near future. However preventive actions require an understanding of the cause. Before the psychological influence on eating can be evaluated a profound understanding of the biological regulatory system and how this interacts with the food consumed is required. On the assumption that food consumption is regulated by interplay between food and genes, the food itself may influence the genes that regulate consumption, hence change the expression levels of the genes regulating food intake. To evaluate the interplay between food and gene expression, the project contained several parts, reflecting different aspects of the area of research. The feeding studies had in common that they were initial trials in a larger project. The results of these will be evaluated and used in combination with further studies. The mice typed for food preference illustrate the complexity of the feeding regulatory system by pointing out the differences between individuals even in a relatively small group of animals. Mice in general like food high in fat and here the animals that showed a preference for sugar also showed a significant increase in their intake of chow. Since chow consists mainly of carbohydrates the results might indicate a preference not for sucrose in particular but for carbohydrates in general. The effect this may have on other studies is still unclear as further studies are needed to determine whether the difference may be the result of an innate genetic difference. Leucine has been previously shown to reduce the total caloric intake. When given in combination with palatable food the addition of Leucine primarily reduced the intake of chow. From a dietary perspective this would translate to a preference to sweets and fast food at the expense of food with more nutritious content. The RT-PCR analysis’s gives clues to how the energy regulatory circuitry responds to the intake of selected macronutrients. When it comes to gene expression there is a significant effect of macronutrients on the gene expression levels. The common theme for many of the genes tested seems to be down regulation of satiety signals, as if to support over feeding on palatable diets and in many cases sucrose in particular. The intake of macronutrients such as sugar or fat has been showed to have an effect on the feeding regulatory circuitry, demonstrated by the change in gene expression levels. The response to said macronutrients is site specific which is clearly shown both by RTPCR analysis of samples from different parts of the brain, such as the brainstem or hypothalamus, and by immunohistochemistry of selected areas. The immunohistochemistry also confirms that the novel Oxytocin receptor-antagonist, who is injected IP, actually passes over the blood-brain barrier and has an actual affect on the regions of interest. The areas affected by the antagonist can be visualized and identified through the staining of active sites.
Gabriel, Luke R. "Dynamic Regulation at the Neuronal Plasma Membrane: Novel Endocytic Mechanisms Control Anesthetic-Activated Potassium Channels and Amphetamine-Sensitive Dopamine Transporters: A Dissertation." eScholarship@UMMS, 2013. http://escholarship.umassmed.edu/gsbs_diss/725.
Повний текст джерелаCrowley, Jessica Lynn. "Role of Supervillin, a Membrane Raft Protein, in Cytoskeletal Organization and Invadopodia Function." eScholarship@UMMS, 2009. https://escholarship.umassmed.edu/gsbs_diss/406.
Повний текст джерелаYunkun, Wu. "X-ray crystal structures of yeast heat shock proteins and mitochondrial outer membrane translocon member Tom70p." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2007. https://www.mhsl.uab.edu/dt/2009r/wu.pdf.
Повний текст джерелаTrueman, Steven F. "Insights Into ER Translocation Channel Gating. Structural Regulation of the Transition Between the Closed and Open Channel Conformations: A Dissertation." eScholarship@UMMS, 2011. https://escholarship.umassmed.edu/gsbs_diss/576.
Повний текст джерелаLaliberte, Jason P. "Role of Host Cellular Membrane Raft Domains in the Assembly and Release of Newcastle Disease Virus: A Dissertation." eScholarship@UMMS, 2008. https://escholarship.umassmed.edu/gsbs_diss/360.
Повний текст джерелаLai, Yinzhi. "CPLA2 key enzyme for astrocytic cell membrane phase property change induced by abeta /." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/5992.
Повний текст джерелаThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on April 14, 2008) Includes bibliographical references.
Forbrig, Enrico [Verfasser], Peter [Akademischer Betreuer] Hildebrandt, Peter [Gutachter] Hildebrandt, Thomas [Gutachter] Gutsmann, and Peter [Gutachter] Hegemann. "Investigation of membrane-active peptides and proteins by vibrational spectroscopy / Enrico Forbrig ; Gutachter: Peter Hildebrandt, Thomas Gutsmann, Peter Hegemann ; Betreuer: Peter Hildebrandt." Berlin : Technische Universität Berlin, 2018. http://d-nb.info/1165138891/34.
Повний текст джерелаCorey, Elizabeth Ann. "Characterization of the Relationship Between Measles Virus Fusion, Receptor Binding, and the Virus-Specific Interaction Between the Hemagglutinin and Fusion Glycoproteins: a Dissertation." eScholarship@UMMS, 2006. https://escholarship.umassmed.edu/gsbs_diss/221.
Повний текст джерелаGatlin, Jesse C. "Eicosanoid-mediated repellent signaling in the nerve growth cone : a role for the PKC substrate MARCKS /." Connect to full text at ProQuest Digital Dissertations. IP filtered, 2005.
Знайти повний текст джерелаTypescript. Includes bibliographical references (leaves 123-141). Free to UCDHSC affiliates. Online version available via ProQuest Digital Dissertations;
Wang, Peng. "Studies on E. Coli Membrane Protein Biogenesis: Mechanism of Signal Peptide Peptidase A and the Influence of YiDC Depletion on Cellular Processes." Columbus, Ohio : Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view.cgi?acc%5Fnum=osu1243982038.
Повний текст джерелаMalaby, Heidi L. H. "Identification of Molecular Determinants that Shift Co- and Post-Translational N-Glycosylation Kinetics in Type I Transmembrane Peptides: A Dissertation." eScholarship@UMMS, 2014. https://escholarship.umassmed.edu/gsbs_diss/702.
Повний текст джерелаMalaby, Heidi L. H. "Identification of Molecular Determinants that Shift Co- and Post-Translational N-Glycosylation Kinetics in Type I Transmembrane Peptides: A Dissertation." eScholarship@UMMS, 2004. http://escholarship.umassmed.edu/gsbs_diss/702.
Повний текст джерелаHarrington, Jane Colleen. "Regulation of Reactive Nitrogen Species (RNS) Metabolism and Resistance Mechanisms in Haemophilus influenzae: A Dissertation." eScholarship@UMMS, 2008. https://escholarship.umassmed.edu/gsbs_diss/401.
Повний текст джерелаZhang, Jin. "Macromolecular Interactions in West Nile Virus RNA-TIAR Protein Complexes and of Membrane Associated Kv Channel Peptides." Digital Archive @ GSU, 2013. http://digitalarchive.gsu.edu/chemistry_diss/81.
Повний текст джерелаParton, Daniel L. "Pushing the boundaries : molecular dynamics simulations of complex biological membranes." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:7ab91b51-a5ae-46b4-b6dc-3f0dd3f0b477.
Повний текст джерелаChen, Minyong. "Deciphering the Role of YidC in Bacterial Membrane Protein Insertion." The Ohio State University, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=osu1039101039.
Повний текст джерелаCheng, Shiyuan. "Membrane assembly studies of subunit h : leader peptidase and m13 procoat proteins in Escherichia coli /." The Ohio State University, 1992. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487777901659517.
Повний текст джерелаSuwal, Shyam, and Shyam Suwal. "Fractionation of Peptides from Protein Hydrolysate by Electrodialysis with Filtration Membrane : process optimization, Fouling characterization and Control mechanisms." Doctoral thesis, Université Laval, 2015. http://hdl.handle.net/20.500.11794/26619.
Повний текст джерелаDes peptides bioactifs ont déjà été fractionnés par électrodialyse avec membrane de filtration (ÉDMF) à partir d’hydrolysats de sous-produits de crabe des neiges. L’optimisation des paramètres apparaît maintenant indispensable pour perfectionner le procédé. Ainsi, le taux de migration des peptides, leur sélectivité et l'évolution des paramètres électrodialytiques ont été étudiés pour différents paramètres (configuration, concentration en KCl et types de champ électrique). La configuration (2) de la cellule d’ÉDMF comprenant deux compartiments d'alimentation et un compartiment de récupération a démontré des valeurs de champ électrique local relativement stables par rapport à la configuration (1) constituée d’un compartiment d’alimentation et de deux compartiments de récupération. Des peptides contenant des glutamates, des aspartates, et des glycines ont été séparés avec la configuration 1 et des peptides composés d’arginines et de lysines avec la configuration 2. Un taux de migration peptidique de 13,76 ± 3,64 g/m2h a été obtenu par le maintien constant de la conductivité des solutions. La sélectivité a été accrue en augmentant la concentration en KCl de 1 à 5 g/L dans le compartiment de récupération. Une augmentation de la force ionique a amplifié la charge de surface, agrandissant ainsi la taille effective des pores et réduisant la couche d'hydratation de la membrane d’ultrafiltration. Toutefois, les membranes échangeuses d’anions et de cations ont été colmatées par des peptides et des acides aminés et détériorées pendant l’ÉDMF. Pour résoudre ces problèmes, l’effet de l’application du champ électrique pulsé (PEF) et de l'inversion de polarité (PR) a été étudié. Le taux de migration des peptides n'a pas été affecté sauf avec PR à 40 V. La sélectivité a été maximale avec PEF à 20 V. La dissociation de l'eau a été réduite tout en conservant les propriétés physico-chimiques des membranes grâce à l’application du PEF et de la PR par rapport au courant continu (DC). En outre, la plus faible quantité d'énergie a été consommée avec le PEF. Par conséquent, il a été possible d’optimiser la technologie d’ÉDMF du point de vue de l’efficacité énergétique, de la sélectivité peptidique et de l’encrassement membranaire grâce à l’application du PEF et tout en maintenant la conductivité électrique des solutions.
Bioactive peptides were efficiently separated by using electrodialysis with filtration membrane (EDFM) from snow crab byproduct hydrolysate. Meanwhile, optimization of parameters is indispensable for scaling-up. The peptide migration rate and selectivity as well as evolution of electrodialytic parameters were studied with different parameters such as EDFM cell configuration, KCl concentration and type of electric field. The EDFM stack with two feed and one recovery compartments (configuration 2) has relatively stable electric field strengths (local) than the configuration with one feed and two recovery compartments (configuration 1). Peptides containing anionic amino acids: glutamic and aspartic acid as well as glycine and cationic amino acids: arginine and lysine were fractionated using configuration 1 and 2, respectively. Maintenance of solution conductivity upheld the local electric field and peptide migration throughout the treatment resulting in a higher peptide migration rate of 13.76±3.64 g/m2.h never observed so far. The selectivity of cationic peptides containing arginine and lysine increased significantly with increase in KCl concentration from 1 to 5 g/L. An increase in ionic strength amplified the surface charge density of filtration membrane subsequently increasing effective pore size and reducing hydration layer. However, both anion- and cation-exchange membranes were fouled by peptides and amino acids and were deteriorated during EDFM treatment. To address these problems, the effect of applying pulsed electric field (PEF) and polarity reversal (PR) was studied. The peptide migration rate was unaffected among PEF, PR and DC modes except with PR at 40 V. The selectivity of cationic peptides was maximum with PEF at 20 V. Fouling and water dissociation were significantly reduced and physicochemical properties of IEMs were better-protected with PEF and PR than DC. Moreover, the least amount of energy was consumed with PEF mode. Therefore, the parameters affecting EDFM process were optimized in terms of energy efficiency, selectivity and lower deterioration of membranes by applying PEF regime with configuration 2 and maintaining the constant electrical conductivity of solutions.
Bioactive peptides were efficiently separated by using electrodialysis with filtration membrane (EDFM) from snow crab byproduct hydrolysate. Meanwhile, optimization of parameters is indispensable for scaling-up. The peptide migration rate and selectivity as well as evolution of electrodialytic parameters were studied with different parameters such as EDFM cell configuration, KCl concentration and type of electric field. The EDFM stack with two feed and one recovery compartments (configuration 2) has relatively stable electric field strengths (local) than the configuration with one feed and two recovery compartments (configuration 1). Peptides containing anionic amino acids: glutamic and aspartic acid as well as glycine and cationic amino acids: arginine and lysine were fractionated using configuration 1 and 2, respectively. Maintenance of solution conductivity upheld the local electric field and peptide migration throughout the treatment resulting in a higher peptide migration rate of 13.76±3.64 g/m2.h never observed so far. The selectivity of cationic peptides containing arginine and lysine increased significantly with increase in KCl concentration from 1 to 5 g/L. An increase in ionic strength amplified the surface charge density of filtration membrane subsequently increasing effective pore size and reducing hydration layer. However, both anion- and cation-exchange membranes were fouled by peptides and amino acids and were deteriorated during EDFM treatment. To address these problems, the effect of applying pulsed electric field (PEF) and polarity reversal (PR) was studied. The peptide migration rate was unaffected among PEF, PR and DC modes except with PR at 40 V. The selectivity of cationic peptides was maximum with PEF at 20 V. Fouling and water dissociation were significantly reduced and physicochemical properties of IEMs were better-protected with PEF and PR than DC. Moreover, the least amount of energy was consumed with PEF mode. Therefore, the parameters affecting EDFM process were optimized in terms of energy efficiency, selectivity and lower deterioration of membranes by applying PEF regime with configuration 2 and maintaining the constant electrical conductivity of solutions.
Li, Xiaoman. "Study on memapsin 2 cleavage properties and its interacting proteins." Oklahoma City : [s.n.], 2010.
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