Дисертації з теми "Membrance domains"
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Gutlederer, Erwin Johann. "On the morphology of vesicles. - [überarb. Diss.]." Universität Potsdam, 2007. http://opus.kobv.de/ubp/volltexte/2007/1506/.
Повний текст джерелаDie vorliegende Arbeit enthält theoretische Untersuchungen zur Morphologie und statistischen Mechanik von Vesikeln. Es wird die Gestalt homogener fluider Vesikel und inhomogener Vesikel mit fluiden und festen Membrandomänen berechnet. Der Einfluss thermischer Fluktuationen wird untersucht. Die erzielten Ergebnisse beziehen sich auf mesoskopische Längenskalen und basieren auf einem geometrischen Membranmodell, in welchem die Vesikelmembran als statische, beziehungsweise thermisch fluktuierende Fläche beschrieben wird. Die Arbeit besteht aus drei Teilen. Im ersten Teil werden homogene fluide Vesikel betrachtet. Das Interesse gilt dem thermisch induzierten Morphologieübergang zwischen prolaten und oblaten Vesikelformen. Mit Hilfe von Monte-Carlo-Simulationen wird ein freies Energieprofil für diese Vesikel ermittelt. Es kann gezeigt werden, dass die Formumwandlung zwischen prolaten und oblaten Formen kontinuierlich verläuft und mit keiner freien Energiebarriere verbunden ist. Der zweite und dritte Teil beschäftigt sich mit inhomogenen Vesikeln, die intramembrane Domänen enthalten. Ausgangspunkt und Motivation der Berechnungen sind experimentelle Studien über Domänbildung in ein- oder mehrkomponentigen Vesikelmembranen, bei denen Phasentrennung stattfindet und unterschiedliche Membranphasen koexistieren. Die dabei auftretenden Domänen unterscheiden sich hinsichtlich ihrer Membranstruktur (fest, fluid). Diese beeinflusst die Form der Domäne und des gesamten Vesikels auf entscheidende Weise. Im zweiten Teil werden Vesikel untersucht, bei denen feste und fluide Membrandomänen koexistieren, Teil drei widmet sich Vesikeln mit zwei koexistierenden fluiden Membranphasen. In Abhängigkeit von Materialparametern werden durch Minimierung der Membranenergie die Grundzustandsformen von Vesikeln mit einfachen und komplexen Domänenformen bestimmt. Die Ergebnisse werden in Morphologiediagrammen zusammengefasst. Dabei werden bisher unbekannte Morphologieübergänge zwischen Vesikeln mit unterschiedlichen Domänformen beobachtet. Die Auswirkungen thermischer Fluktuationen auf die Vesikel und die Form ihrer Domänen werden mittels Monte-Carlo-Simulationen untersucht.
Oldham, Alexis Jean. "Modulation of lipid domain formation in mixed model systems by proteins and peptides." View electronic thesis, 2008. http://dl.uncw.edu/etd/2008-1/r1/oldhama/alexisoldham.pdf.
Повний текст джерелаWilkinson, Debbie Isabelle. "Visualisation of osteoclast membrane domains." Thesis, University of Aberdeen, 2010. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158808.
Повний текст джерелаDaste, Frédéric. "Function and regulation of coiled‐coil domains in intracellular membrane fusion." Thesis, Sorbonne Paris Cité, 2015. http://www.theses.fr/2015PA05T001.
Повний текст джерелаThe molecular mechanisms involved in membrane fusion have been extensively studied for the past thirty years. Our current understanding of this phenomenon is mainly based on results obtained by (i) the development of physical models describing the fusion of membranes, (ii) structural and mechanistic investigations on fusion proteins of enveloped viruses and (iii) studies of SNARE protein-mediated intracellular fusion events of eukaryotic cells. Discovery of the SNARE complex was the outcome of interdisciplinary works which involved a wide range of techniques including yeast genetics, electrophysiology, molecular biology, cell-free biochemistry, adhesion/fusion biophysics and imaging. Taking advantage of the paradigms and biophysical techniques that emerged from these studies, we investigated the function and regulation of coiled-coil domains in intracellular fusion processes involving Longin-SNAREs or Mitofusins, two fusion protein machineries whose exact mode of action still remains unclear. A comprehensive understanding of the molecular mechanisms of membrane fusion requires the in vitro reconstitution of fusion proteins into a wide variety of membrane environments with defined and tunable biophysical properties. Ideally, these membrane systems should allow the experimentalists to control the lipid and protein composition as well as the membrane topology, to account for the variability observed across cellular fusing compartments. Reconstitution into liposomes offers amazing flexibility with the capacity to vary most of these relevant parameters, and to create a minimal environment in which membrane and/or soluble factors can be added, one at a time or in combination, to reveal their role with clarity. We have set up the in vitro reconstitution of proteins into various artificial membrane platforms for both systems (the Longin-SNAREs TI-VAMP and Sec22b and the coiled-coil domains of Mitofusins) and performed biochemical assays to gain insight into how these proteins execute their functions. The long-term goal of this project is to compare the molecular mechanisms of SNARE and Mitofusin fusion machineries and thus reveal structural and functional similitudes between (i) their core fusion proteins, and (ii) their regulatory factors
Jean-François, Frantz. "Vers un nouveau mode d’action de peptides antimicrobiens structurés en feuillets ß : formation de domaines membranaires par la cateslytine." Thesis, Bordeaux 1, 2008. http://www.theses.fr/2008BOR13638/document.
Повний текст джерелаThe antimicrobial peptide Cateslytin (bCGA RSMRLSFRARGYGFR ) is a five positively charged arginin rich peptide known to inhibit the release of catecholamine in chromaffin granules. Although biological data showed that it is able to inhibit the growth of several microorganisms such as bacteria, yeast and Plasmodium falciparum parasite involved in malaria, the mechanism of action has not been yet studied. In order to better understand both targeting and selectivity of this peptide towards microorganisms, model membranes of variable compositions have been chosen to respectively mimic microorganisms or mammalian membranes. Structural studies have been performed using polarised ATR-FTIR, circular dichroïsm and high resolution NMR Membrane dynamics has been followed using deuterium labelled lipids and solid state NMR Patch clamp experiments were also performed on lipid vesicles to measure channe conductivity. All-atom molecular dynamics on hydrated peptide-lipid membrane systems was also used to assess the interaction from the atomic level. Main results from this interdisciplinary approach are three-fold. i) Electric current passages through membranes demonstrate permeation akin to pore formation. ii) Peptide-induced formation of rigid domains mainly made of negatively charged lipids is found. iii) Peptide antiparallel ß-sheets are observed preferentially with negatively charged lipids mimicking microorganism membranes. The general picture leads to the proposal that membrane destabilization/permeation is promoted by rigid domains stabilised by peptide ß-sheets
Goulding, Rebecca Ellen. "Membrane localization of RasGRPs by C1 domains." Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/24211.
Повний текст джерелаHeadlam, Madeleine Joyce. "Cytochrome P450scc (CYP11A1) : effects of glycerol and identification of the membrane binding domain." University of Western Australia. School of Biomedical and Chemical Sciences, 2004. http://theses.library.uwa.edu.au/adt-WU2004.0065.
Повний текст джерелаSalkhordeh, Mahmoud. "Localization of membrane binding domains in synapsin I." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ57790.pdf.
Повний текст джерелаSalkhordeh, Mahmoud Carleton University Dissertation Biology. "Localization of membrane binding domains in synapsin I." Ottawa, 2000.
Знайти повний текст джерелаBrechin, C. "14-3-3 proteins and cholesterol-dependent membrane domains." Thesis, University of Edinburgh, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.641914.
Повний текст джерелаBdiri, Myriam. "Etude du décolmatage, par procédés chimiques et biologiques, des membranes échangeuses d'ions utilisées en électrodialyse dans le domaine agroalimentaire." Thesis, Paris Est, 2018. http://www.theses.fr/2018PESC1070.
Повний текст джерелаConventional electrodialysis (ED) is mainly based on the specific action of ion exchange membranes (IEMs) and is widely used in food industry for tartaric stabilization of wines, deacidification and treatment of fruit juices, demineralization of whey or elimination and fractionation of milk proteins. The organic fouling, accentuated by the complex composition of the food effluents and their richness in phenolic compounds, represents a major limitative factor of the process efficiency and the IEMs performance. This phenomenon causes a decrease in the selectivity of membranes, an increase in their electrical resistance and reduces the energy efficiency of the process leading to economic losses in industry. This study mainly consists in studying the IEMs cleaning by chemical and biological methods. Two batches of new membranes (cation- (CEMs) and anion-exchange membranes (AEMs)) and five batches of used ones (3 CEMs and 2 AEM) with different durations of use in ED units in food industry -confidential application- have been studied. All the samples have been previously characterized to determine their physicochemical parameters (ion-exchange capacity (IEC), thickness (Tm), electrical conductivity (km), contact angle (θ), water content (WC) and the volume fraction of the inter-gel solution (f2) resulting from the study of the micro heterogeneous model), structure and morphology by FTIR spectroscopy, optical microscopy, scanning electron microscopy and mechanical by tensile strength tests. The direct and indirect effects (caused by the regular cleaning operations in industry) of fouling as well as the anisotropy of the membranes mechanical properties have been highlighted. Non-aggressive and environmentally friendly cleaning methods have been experimentally tested in ex-situ static mode: Saline solutions (35 g.L-1 NaCl and reconstituted seawater), hydro-alcoholic solution (12% water-ethanol mixture, pH = 3,5) and biological solutions using 3 categories of enzymatic agents (Rohalase BX-BXL, β-glucanase / Corolase 7089, endo-peptidase / Tyrosinase, polyphenol oxidase) whose operating conditions of optimal enzymatic activity have been determined. The evolution of IEC, km, θ and f2 were followed in function of the cleaning duration. Saline solutions have a negligible effect on intern cleaning but remain efficient for extern cleaning. However, the application of the hydro-alcoholic solution and enzyme solutions have been found to be efficient for both intern and extern cleaning and led to significant recoveries of the studied parameters. It has been shown that phenolic compounds, the principal constituents of treated effluents, are mainly responsible for MEIs fouling. Apparently, they form dense colloidal nanoparticles not permeable for ions within membrane meso- and macropores, not penetrating into micropores. A modification of the micro heterogeneous model under this assumption allowed an adequate interpretation of km and the modelization of structural modifications of the inter-gel phase generated by the fouling mechanisms by polyphenols and explained the reasons why the f2app decreases. An extraction method using a mixture of solvents (25% V/V, acetone/methanol/ isopropanol/water) was developed and made it possible to extract certain phenolic compounds from different batches of used CEMs and AEMs that were identified by high performance liquid chromatography. It has also been demonstrated that the interactions between the phenolic compounds and the polymer matrix are mainly governed by the stacking of aromatic rings and electrostatic interactions of the CH-pi and pi-pi type as well as the hydrogen bonds
Regula, Camille. "Etude du vieillissement des membranes dans le domaine de l’agroalimentaire : production d’eau potable et filtration du lait." Thesis, Aix-Marseille, 2013. http://www.theses.fr/2013AIXM4313.
Повний текст джерелаIn Food Industries, using membrane processes, contact with cleaning chemicals is believed to play an important role in membrane ageing. In this thesis, polysulfone hollow fibers and polyethersulfone spiral wound membranes were used to simulate the industrial cleaning in static conditions. Ageing of the membrane was mimicked by immersing samples in solutions containing commercial detergents with various concentrations, temperatures and soaking times defined by experimental designs. In an innovative way in the chemical membranes ageing research, an approach based on methodological tools has been realized for different formulated detergents (alkaline, acidic, enzymatic, oxidant and biocide). The main interest is to achieve a relevant ageing pattern without using an accelerated ageing protocol which has been proved to be not relevant. Results allow modeling macroscopic ageing involved by each detergent. Those modifications have been studied as well at the macroscopic scale as at the microscopic scale in order to put membrane ageing in a global perspective: flux, mechanical properties, surface properties, polymer modifications. Specifications of using can be advised according to membranes and products which represent a real oversight handbook about membrane cleaning for industrial users
Kelm, Sebastian. "Structural modelling of transmembrane domains." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:b4c9fba9-ee25-469b-8baf-b7c1d70c9d05.
Повний текст джерелаLeidy, Chad. "Thermotropic behavior of lipid domains in model membranes /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2002. http://uclibs.org/PID/11984.
Повний текст джерелаRink, Jochen C. "Rab-domain dynamics in endocytic membrane trafficking." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2005. http://nbn-resolving.de/urn:nbn:de:swb:14-1117095871452-66763.
Повний текст джерелаKreder, Rémy. "Sondes moléculaires multifonctionnelles pour l'imagerie de fluorecence de membranes cellulaires." Thesis, Strasbourg, 2015. http://www.theses.fr/2015STRAJ006/document.
Повний текст джерелаBased on rational molecular design, we design new membrane probes that enable fluorescence imaging of cell plasma membrane organization. In this work, we first synthesized a toolkit, based on solvatochromic Nile Red dye and Black Hole Quencher-2, that can stain specifically ordered and disordered lipid domains (rafts) and identify them by the emission color. Cellular studies with these probes suggested that the plasma membrane is composed of two distinct phases. Then,with the idea to make Nile Red-based probes compatible with serum medium and fixable by formaldehyde/glutaraldehyde, we modified previously developed probe NR12S with PEG and aminogroups, respectively. Surprisingly, the PEGylated probe is quickly internalized inside the cell and the amino-derivative aggregates with the fixing agent. On the other hand, based on Nile Red we designed probes capable to detect a given receptor and visualize its lipid environment. Initially, we obtained probes that can turn-on fluorescence on binding to the oxytocin GPCR receptor. Then, we conjugated NR12S through a PEG(12) spacer to the ligand of intergrin, RGD. The first data show that the molecule can bind to the membrane and detect the lipid order, though cellular studies have to be completed. We also worked on fluorogenic (turn-on) membrane probes for multi-color imaging. Based on blue 3-methoxychromone dyes, we obtained probes that are brighter and more photostable than the originally developed probe based on 3-hydroxychromone (F2N12S). Due to large Stocks shift, they enabled cell membrane imaging with minimal auto-fluorescence in the blue spectral region, compatible with common green and red probes. At the end, based on squaraine fluorophore, we developed three new probes operating in the far red region, which is also very interesting for in vitro and in vivo imaging. These dyes show a parallel orientation with the lipid membrane, while the cellular experiments point out that only the probe with two anchor groups is able to stain stably the plasma membrane. The probes developed here are expected to be used for lipid rafts research as well as for super-resolution and multi-color imaging of living cells
Karathanassis, Dimitrios. "Structural insights into membrane binding by phox homology (PX) domains." Thesis, University of Cambridge, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.619724.
Повний текст джерелаBrechin, Carolyn. "SNAREs, 14-3-3 proteins and cholesterol dependent membrane domains." Thesis, University of Edinburgh, 2007. http://hdl.handle.net/1842/29276.
Повний текст джерелаHagelbäck, Johan, and Kenny Svensson. "Locating transmembrane domains in protein sequences." Thesis, Blekinge Tekniska Högskola, Institutionen för programvaruteknik och datavetenskap, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-2752.
Повний текст джерелаYandrapalli, Naresh. "Role of HIV-1 Gag protein multimerization in the generation of nanodomains in lipid membranes." Thesis, Montpellier, 2016. http://www.theses.fr/2016MONTT097/document.
Повний текст джерелаGag polyprotein of HIV-1 is made of four main domains Matrix (MA), Capsid (CA), Nucleocapsid (NC), and P6 and is the prime orchestrator of virus assembly that occurs during the late phase of replication. It is well known that Gag interacts with host cell lipids and self-assemble along the inner-leaflet of the plasma membrane in order to generate virus like particles (VLPs). Budding of these VLPs out of the living cell is described to be ESCRT dependent. Structural, functional and simulation based studies has shown that Gag membrane binding is mediated by a bipartite interaction. One specific electrostatic interaction, between the highly basic region (HBR) of its MA domain and the host cell acidic lipid phosphatidyl inositol bisphophate (PI(4,5)P2), plus a hydrophobic interaction through Gag’s myristate insertion in the plasma membrane. It is still an opened question whether Gag would specifically recognize pre-existing lipid domains such as rafts to optimize its multimerization or, on the contrary, would reorganize lipids during its multimerization. During my Ph.D. I explored the second hypothesis using purified myr(-) Gag protein and model membranes containing fluorescently labelled PI(4,5)P2.Bonding experiments have shown strong affinities of these purified proteins towards PI(4,5)P2 containing lipid bilayers. Using PI(4,5)P2 fluorescence self-quenching properties, I found that multimerization Gag generates PI(4,5)P2/Cholesterol enriched nanoclusters. On the opposite, sphingomyelin was excluded from these nanoclusters. In addition to this, using a fluorescently labelled myr(-) Gag, I have observed its preferable partitioning into lipid disordered (Ld) phases of giant unilamellar vesicles (GUVs). Further, possibility of whether HIV-1 Gag alone, as a minimal system, can induce the formation of vesicles on PI(4,5)P2/PS containing supported lipid bilayers (SLBs) & GUVs was tested. Using quartz crystal microbalance (QCM-D) and fluorescence microscopy techniques, I monitored the self-assembly of HIV-1 Gag with time and found that Gag was sufficient to generate membrane curvature and vesicle release. Moreover, using mutants of this protein, I found that having MA and CA domain is enough for Gag to produce vesicle like structures. Taken together, these results suggest that binding and multimerization of Gag protein does not occur in pre-existing lipid domains (such as “rafts”) but this multimerization is more likely to induce PI(4,5)P2/Cholesterol nanoclusters. This nanophase separation could locally play a role in the membrane curvature needed for the budding of the virus
Smith, Karen Louise. "Structure and function of neuronal GPI domains." Thesis, King's College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313392.
Повний текст джерелаKing, Gavin W. "Investigating helix-helix interactions in the transmembrane domains of membrane proteins." Thesis, University of Warwick, 2009. http://wrap.warwick.ac.uk/3157/.
Повний текст джерелаKucherak, Oleksandr. "Development of new fluorescent membrane probes for apoptosis and raft domains." Strasbourg, 2011. http://www.theses.fr/2011STRA6056.
Повний текст джерелаFluorescent dyes are of great importance for investigation of biological processes. Recently, the first ratiometric fluorescent probe for apoptosis detection (F2N12S) was developed in our laboratory. The aim of the present work was to develop improved fluorescent apoptosis probes that will overcome the drawbacks of F2N12S. Primarily, we performed systematic studies of F2N12S in model membranes and cells to better understand the mechanism of its response to apoptosis. We found that in addition to surface charge, the membrane hydration and phase state are changed on apoptosis, thus explaining the spectroscopic response of the probe. On the second step, we have synthesized about 20 new membrane probes, which were classified into three types according to the experiments in model membranes. The first type of dyes showed nearly no sensitivity to the phase state while their sensitivity to surface charge was much higher (>3-fold) as compared to F2N12S. The second type showed improved sensitivity both to the surface charge and to the phase state of the lipid bilayers. The third type, which was based on Nile Red, was sensitive only to the phase state. Basing on these results we proposed general principles for the design of fluorescent membrane probes. Finally we described two new classes of environment-sensitive fluorophores on the base of fluorene and 3- methoxychromone units for further construction of new advanced membrane probes. These new fluorophores showed attractive spectroscopic properties, such as high brightness and photostability as well as an exceptional fluorescence solvatochromism. The first attempts to convert these fluorophores into membrane probes were described
Björkholm, Patrik. "Protein Interactions from the Molecular to the Domain Level." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-101795.
Повний текст джерелаAt the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 3: Manuscript.
Fuhrer, Andrew B. "The Role of Lipid Domains and Sterol Chemistry in Nanoparticle-Cell Membrane Interactions." Ohio University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1596569401131742.
Повний текст джерелаGeorgiev, Alexander. "Membrane Stress and the Role of GYF Domain Proteins." Doctoral thesis, Stockholm : Department of Biochemistry and Biophysics, Stockholm university, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-7764.
Повний текст джерелаRubin, Darrell. "ACUTE REGULATION OF GLUT1 FUNCTION: THE ROLE OF DETERGENT-RESISTANT MEMBRANE DOMAINS." Connect to text online, 2004. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=case1087996732.
Повний текст джерелаLaurent, Nelson. "Caractérisation des mécanismes régulant les modifications de la structuration membranaire induites dans la signalisation des réponses de défense des plantes." Thesis, Bourgogne Franche-Comté, 2020. http://www.theses.fr/2020UBFCK026.
Повний текст джерелаSpatial distribution of pasma membranes (PM) components is tightly regulated to provide the cell an optimal physiological state. The level of order degree is a suitable parameter to study PM organization, reflecting the intensity of interactions taking place between PM components and so the level of their packing. During our work, we used the environment sensitive probe di-4-ANEPPDHQ to assess the level of order degree of tobacco BY-2 cells PM in different situations: during the time-course of cell regeneration and in the particular case of an elicitation by cryptogein.We measured that the level of order degree of PM is modulated during the slow process of cell regeneration. This regulation isn’t related to the cell wall neo-synthesis neither the cell morphology, but is correlated to the differentiation state. The mapping of the level of order degree along the PM perimeter of protoplasts and suspension cells revealed a global common organization with a mosaic like distribution of domains (288X288 nm) exhibiting high diversity of level of order degree. The two models differed by the organization of this mosaic, the cell PM exhibiting enrichment in highest ordered domains in a specific area between two adjacent cells. Then, we discus about a possible involvement of membrane trafficking in the regulation of the level of order degree allowing specific targeting of lipids/proteins, and subsequent cellular identity acquisition.A pharmacological approach allowed the identification of the reactive oxygen species involved in the PM reorganization observed at the surface of tobacco BY-2 cells in response to cryptogein. We report that H2O2 has similar effects on the PM level of order degree compared to cryptogein’s one, both acting doses dependently. Furthermore, a partial inhibition of the H2O2 accumulation occurring in response to cryptogein is linked to a partial inhibition in the increase of the level of order degree usually induced by cryptogein. Those results suggest that H2O2 could finely regulates The PM level of order degree during the response of tobacco cells to cryptogein. The potential mechanisms involved has been studied. Two current hypotheses were studied in parallel, and both were rejected. The first one involves a modification of PM composition by an arrival of ordered domain to the PM. In the second one, a de novo formation of PM component structure. So, we discuss about a new role for H2O2, which would act directly on the PM level of order degree
Laudon, Hanna. "Functional domains in the Alzheimer's disease-associated presenilin 1 protein /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7140-085-0/.
Повний текст джерелаStie, Jamal Talal. "Modulation of the Plasma Membrane Domain Structure of Human Neutrophils." Thesis, Montana State University, 2006. http://etd.lib.montana.edu/etd/2006/stie/StieJ0806.pdf.
Повний текст джерелаBaranova, Ekaterina. "Electron crystallography of the membrane domain of respiratory complex I." Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.612931.
Повний текст джерелаXu, Yuanda. "Thermodynamic and Hydrodynamic Coupling Effects on Compositional Lipid Domains in Membrane Stack Systems." Thesis, Princeton University, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10642189.
Повний текст джерелаThis dissertation will focus on my work in biophysics, and my work in mean field games and glucose predictive analysis will not be presented. Several problems relating to the effects of thermodynamic coupling and hydrodynamic coupling within the membrane stack system are discussed. Three theoretical approaches are employed and proposed to study the membrane stack system: a diffuse-interface approach is utilized for numerical simulations; a coarse-grained sharp-interface approach is utilized to provide physical understanding of various kinetics; a hybrid intermediate sharp-interface approach is adopted to study the domain coalescence in the absence of diffusion.
In the first part of the thesis, we discuss the thermodynamic coupling in membrane stack systems. Comprehensive analyses are presented to understand the accelerated coarsening kinetics with respect to single layer and long-range alignment. Numerical simulations are conducted for three systems, namely a diffusion dominated system, an advective interlayer friction dominated system, and an advective membrane viscosity dominated system. Experimental results regarding the advective interlayer friction dominated system are supported by simulations. We investigate the mechanism of the enhanced coarsening kinetics in membrane stack systems and the relationship between the coarsening process and vertical alignment. An intuitive understanding along with analytical explanations are further presented. Moreover, numerical results regarding the critical mixture are also discussed.
We then investigate the interfacial fluctuation behavior within membrane stack systems. The hydrodynamic coupling is found to play a significant role and several physical length scales are found to be crucial. Both a sharp-interface approach and a diffuse-interface approach are employed to numerically simulate decay of interface fluctuations in representative two-membrane systems.
To measure the thermodynamic coupling in experiments, the hydrodynamic force needs to be quantified, especially for the non-circular domains. In the last part of this thesis, the drag coefficient relating domain velocity and force acting on the domain is calculated using perturbation theory within two limits: the first limit refers to a domain much larger than the hydrodynamic screening length; the second limit refers to a domain that is much smaller than the hydrodynamic screening length.
Lefevre, Rémy. "Conception et réalisation d'une micropompe intelligente : applications dans le domaine biomédical." Phd thesis, Université de Grenoble, 2013. http://tel.archives-ouvertes.fr/tel-00935192.
Повний текст джерелаThevenin, Damien. "Roles of transmembrane domains in the folding and assembly of the adenosine A2A receptor." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 170 p, 2007. http://proquest.umi.com/pqdweb?did=1260822171&sid=2&Fmt=2&clientId=8331&RQT=309&VName=PQD.
Повний текст джерелаPolonovski, Véronique. "Etude du mécanisme d'action de l'homéoprotéine Engrailed 2 : interaction protéine - protéine avec la protéine à domaine forkhead Foxa2 et interaction protéine - membrane." Paris 6, 2007. http://www.theses.fr/2007PA066490.
Повний текст джерелаPetrov, Eugene P., Rafayel Petrosyan, and Petra Schwille. "Translational and rotational diffusion of micrometer-sized solid domains in lipid membranes." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-139339.
Повний текст джерелаDieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich
Petrov, Eugene P., Rafayel Petrosyan, and Petra Schwille. "Translational and rotational diffusion of micrometer-sized solid domains in lipid membranes." Royal Society of Chemistry, 2012. https://tud.qucosa.de/id/qucosa%3A27824.
Повний текст джерелаDieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
Liang, Ying. "Characterization of Sad1/UNC-84 domain protein 2 (SUN2)." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B36616515.
Повний текст джерелаLiang, Ying, and 梁瑛. "Characterization of Sad1/UNC-84 domain protein 2 (SUN2)." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B36616515.
Повний текст джерелаKongmanas, Kessiri. "Roles of Seminolipid and Its Associated Membrane Domain in Male Fertility." Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/32509.
Повний текст джерелаScott, Angela Michelle. "Mechanism of membrane binding of the C2 domains of conventional protein kinase C isoforms." Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2006. http://wwwlib.umi.com/cr/ucsd/fullcit?p3222056.
Повний текст джерелаTitle from first page of PDF file (viewed September 20, 2006). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 127-132).
Saleh, Mazen T. "Identifying domains of Shiga-like toxin I that are responsible for its membrane translocation." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0002/NQ27715.pdf.
Повний текст джерелаCollinson, Ian Richard. "Studies of the membrane and stalk domains of ATP synthase from bovine heart mitochondria." Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360698.
Повний текст джерелаLu, Ruifeng, and Jean M. Wilson. "Rab14 specifies the apical membrane through Arf6-mediated regulation of lipid domains and Cdc42." NATURE PUBLISHING GROUP, 2016. http://hdl.handle.net/10150/622499.
Повний текст джерелаGrosjean, Kevin. "Microdomaines ordonnés de la membrane plasmique végétale : caractérisation et rôle dans la signalisation associée à la défense." Thesis, Dijon, 2015. http://www.theses.fr/2015DIJOS089/document.
Повний текст джерелаRecent studies have shown the existence of lateral sub-compartmentalization of plant plasmamembrane similar to that of animal cells and yeasts. The aim of this thesis was to provide newelements to characterize this compartmentalization (physical properties of specific domains,mechanisms of their formation, determination of their size, etc...) and to study its role in thephysiology of plant cells.The development spectral confocal microscopy coupled with the use of an environment-sensitiveprobe enabled to obtain the first description at the submicron scale of plasma membrane organizationinto domains exhibiting various physical properties. These domains coexist at the plasma membranesurface of tobacco suspension cells as well as the membrane of vesicles composed of models lipids orcell plasma membrane lipids, purified plasma membrane vesicles, and protoplasts. However,differences in the lateral organization observed in these membranes have shown the importance ofphytosterols which are, through specific interactions with neighboring plant lipids such as GIPCs,essential for local formation of ordered domains. The huge diversity of plant lipids drives thecompartmentalization of the plasma membrane allowing the dynamic segregation of membranecomponents. Sterols greatly increase membrane order, whereas proteins tends to decrease it.Cytoskeleton and cell wall do alter neither presence nor organization of ordered domains of the plasmamembrane. We have also shown that the organization of these domains is transiently modified duringthe early stages of defense signaling cascade. In fact, we have identified changes in overall physicalproperties and fine lateral organization of the membrane caused by various elicitors of defensereactions, including cryptogein, a protein secreted by the oomycete Phytophthora cryptogea. We haveshown that these changes are a generic element of defense signaling cascade and depend onphosphorylation processes; oxidative burst being also a major actor of the control of the increase ofmembrane order observed during the very early stages of the signalling process. Cryptogein, whichexhibits the particular ability to trap sterols, also showed a specific capacity to increase membranefluidity and amplify the intensity of the signalling cascade, as measured by the production of reactiveoxygen species.These results open new perspectives in the understanding of cell-elicitin interactions and provide anew view on the central role of sterol composition in the lateral organization of plant plasmamembrane. They also identify membrane dynamics as a new player in the signalling cascade occurringduring plant defense
Chojnacki, Jakub. "Envelope protein domains of duck hepatitis B virus : role in assembly and infectivity /." Connect to thesis, 2005. http://eprints.unimelb.edu.au/archive/00001738.
Повний текст джерелаTypescript. Includes bibliographical references (leaves 155-177).
Pinto, Belinda Sophia Geyer Pamela Kent. "Understanding the role of LEM domain proteins in Drosophila development." [Iowa City, Iowa] : University of Iowa, 2009. http://ir.uiowa.edu/etd/421.
Повний текст джерелаNikolaus, Jörg. "Hemifusion and lateral lipid domain partition in lipid membranes of different complexity." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://dx.doi.org/10.18452/16437.
Повний текст джерелаMembrane fusion is ubiquitous in life and requires remodelling of two phospholipid bilayers. Fusion likely proceeds through similar sequential intermediates. A stalk between the contacting leaflets forms and radially expands into a hemifusion diaphragm (HD) wherein finally a fusion pore opens up. Direct experimental verification of this key structure is difficult due to its transient nature. Confocal microscopy was used to visualize the fusion of giant unilamellar vesicles (GUVs) comprising negatively charged phosphatidylserine and fluorescent transmembrane (TM) entities in the presence of divalent cations. A complete displacement of TM peptides preceded full fusion. This is consistent with HD formation. Detailed analysis provided proof that the micrometer sized structures are in fact HDs. HD size is dependent on lipid composition and peptide concentration. Lateral lipid domain formation is believed to be essential for sorting and signalling processes in the cell. Liquid ordered (Lo) domains in model systems like GUVs resemble biological rafts enriched in sphingolipids and cholesterol, but their physical properties seem distinct from biological membranes as judged by e.g. lipid order and packing. In this context the sorting of TM anchored influenza virus hemagglutinin (HA) and different lipid anchored Ras proteins is studied in GUVs and giant plasma membrane derived vesicles (GPMVs). Authentic HA or the TM domain peptides were sorted exclusively (GUVs) or predominantly (GPMVs) to the liquid disordered (Ld) domains. Whereas K-Ras was found in the bulk Ld domains, N-Ras diffuses to the Lo/Ld interface. These results are discussed with respect to differences in lipid packing in the different membrane systems and regarding the membrane anchors and their hydrophobic matching. The results suggest that the formation, size and stability as well as the physical properties of lipid domains in biological membranes are tightly regulated by protein-lipid interactions.
Holmlund, Camilla. "Identification and investigations of leucine-rich repeats and immunoglobulin-like domains protein 2 (LRIG2)." Doctoral thesis, Umeå : Department of Radiation Sciences, Oncology, Umeå university, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-33784.
Повний текст джерелаYoung, Mark. "Studies of the transmembrane domain of the human erythrocyte anion exchanger (band 3)." Thesis, University of Bristol, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.340324.
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