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Статті в журналах з теми "Melissopalynological"

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Автор: Grafiati

Опубліковано: 18 травня 2024

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1

SAJWANI, A., S. A. FAROOQ, A. PATZELT, E. A. ELTAYEB, and V. M. BRYANT. "MELISSOPALYNOLOGICAL STUDIES FROM OMAN." Palynology 31, no. 1 (January 1, 2007): 63–79. http://dx.doi.org/10.2113/gspalynol.31.1.63.

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2

Omur Gencay; SORKUN, CELEMLI. "History of melissopalynological methods." Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering 27, no. 2 (2018): 88–92. http://dx.doi.org/10.1501/commuc_0000000201.

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3

Nair, Samira, Boumedienne Meddah, and Abdelkader Aoues. "Melissopalynological Characterization of North Algerian Honeys." Foods 2, no. 1 (March 7, 2013): 83–89. http://dx.doi.org/10.3390/foods2010083.

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4

Karabournioti, Sophia E., Ioannis Tsiripidis, Andreas Thrasyvoulou, and Eleftherios P. Eleftheriou. "Melissopalynological attributes of some Greek thyme honeys." Journal of Apicultural Research 48, no. 2 (January 2009): 104–14. http://dx.doi.org/10.3896/ibra.1.48.2.04.

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5

Dimou, Maria, Chrysoula Tananaki, Georgios Goras, Emmanuel Karazafiris, and Andreas Thrasyvoulou. "Melissopalynological analysis of royal jelly from Greece." Grana 52, no. 2 (June 2013): 106–12. http://dx.doi.org/10.1080/00173134.2012.719538.

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6

TIWARI, SHRADDHA, and P. C. MISRA. "MELISSOPALYNOLOGICAL STUDIES OF DISTRICT KAUSHAMBI UTTAR PRADESH." Indian Journal of Scientific Research 14, no. 2 (January 31, 2024): 7–10. http://dx.doi.org/10.32606/ijsr.v14.i2.00002.

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7

Jiang, Weiwen, Marie-José Battesti, Yin Yang, Élodie Jean-Marie, Jean Costa, Didier Béreau, Julien Paolini, and Jean-Charles Robinson. "Melissopalynological Analysis of Honey from French Guiana." Foods 13, no. 7 (March 31, 2024): 1073. http://dx.doi.org/10.3390/foods13071073.

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Анотація:

Beekeeping directly depends on the floral biodiversity available to honey bees. In tropical regions, where nectar and pollen resources are numerous, the botanical origin of some honey is still under discussion. A precise knowledge of plants foraged by honey bees is useful to understand and certify the botanical origin of honey. In this study, attention was paid to honey samples from the French Guiana Atlantic coast where beekeepers generally place their hives in four types of biotopes: seaside vegetation, mangrove, savannah, and secondary forest. Pollen analysis of 87 honey samples enabled the identification of major plants visited by Africanized honey bees during the dry season (approximately from July to January). Through melissopalynologic analysis, 51 pollen types were identified and classified according to their relative presence. Frequently observed pollens (with relative presence > 50%) in French Guiana kinds of honey were those from Mimosa pudica, Cocos sp., Rhyncospora sp., Avicennia germinans, Paspalum sp., Spermacoce verticillata, Tapirira guianensis, Cecropia sp., Myrtaceae sp., Mauritia flexuosa sp., Solanum sp., and Protium sp. In many honeys, only M. pudica was over-represented (relative frequency > 90%). Color and electrical conductivity in French Guiana honeys exhibit significant variations, with color ranging from 27 mm to 110 mm Pfund, and electrical conductivity ranging from 0.35 to 1.22 mS/cm.

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8

Di Rosa, Ambra, Anna Marino, Francesco Leone, Giuseppe Corpina, Renato Giunta, and Vincenzo Chiofalo. "Characterization of Sicilian Honeys Pollen Profiles Using a Commercial E-Tongue and Melissopalynological Analysis for Rapid Screening: A Pilot Study." Sensors 18, no. 11 (November 21, 2018): 4065. http://dx.doi.org/10.3390/s18114065.

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Анотація:

Honey is usually classified as “unifloral” or “multifloral”, depending on whether a dominating pollen grain, originating from only one particular plant, or no dominant pollen type in the sample is found. Unifloral honeys are usually more expensive and appreciated than multifloral honeys, which highlights the importance of honey authenticity. Melissopalynological analysis is used to identify the botanical origin of honey, counting down the number of pollens grains of a honey sample, and calculating the respective percentages of the nectariferous pollens. In addition, sensory properties are also very important for honey characterization, and electronic senses emerged as useful tools for honey authentication. In this work, a comparison of the results obtained from melissopalynological analysis with those provided by a potentiometric electronic tongue is given, resulting in a 100% match between the two techniques.

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9

Pospiech, Matej, Zdeňka Javůrková, Pavel Hrabec, Helena Čížková, Dalibor Titěra, Pavel Štarha, Simona Ljasovská, et al. "Physico-Chemical and Melissopalynological Characterization of Czech Honey." Applied Sciences 11, no. 11 (May 28, 2021): 4989. http://dx.doi.org/10.3390/app11114989.

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Анотація:

Geographical and botanical origin of honeys can be characterized on the basis of physico-chemical composition, sensory properties and on the basis of melissopalynological analysis. No comprehensive description of the characteristics of Czech honey has been published so far. This study provides insights that are important for correct classification. The study analysed 317 samples of authentic honey from randomly selected localities. Due to the diversity of the landscape, the typical honey of the region is blend honey with a predominance of blossom honey. According to the pollen profile and electric conductivity, the honeys were sorted into the following: Brassica honey (BH), Floral honey (FH), Fruit tree honey (PH), Honeydew (HD), Lime tree honey (LH), Robinia pseudoacacia honey (RH), and Trifolium honey (TH). Physico-chemical properties, including higher carbohydrates, were determined for the honeys and their pollen profiles were examined. The physico-chemical properties and pollen profile are partially in compliance with the description of European monofloral honeys, except for RH and TH. Although they had the highest proportion of acacia pollen, amounting to >10% of all the Czech honeys, these RH honeys differ from the European standard, so they cannot be considered acacia honey. Further, PH honeys and FH polyfloral honeys were described. Most honeys contained a significant proportion of rapeseed pollen, which is one of the common agricultural crops grown in the Czech Republic. All the analysed honeys met the parameters defined by the legislation. Due to direct on-site sampling, honeys were characterized by a low 5-(hydroxymethyl)furfural (HMF) content (3.0 mg/kg) and high diastase activity (24.4 DN). Honeydew honeys had the highest proportion of higher carbohydrates, primarily of Melezitose (4.8 g/100 g) and Trehalose (1.3 g/100 g). The presence of higher carbohydrates was also confirmed in LH for Maltose (4.6 g/100 g) and Turanose (2.4 g/100 g).

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10

Ozturk, Munir, Riza Dalgic, Aykut Guvensen, and Volkan Altay. "An Overview of the Melissopalynological Studies in Turkey." Open Conference Proceedings Journal 4, no. 1 (March 1, 2013): 257. http://dx.doi.org/10.2174/2210289201304010257.

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11

Adamchuk, L. "Melissopalynological studies on honeys from the ukrainian carpathians." Scientific Works of National University of Food Technologies 28, no. 1 (February 2022): 154–64. http://dx.doi.org/10.24263/2225-2924-2022-28-1-15.

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12

Rosdi, Izzah Nasuha, Kirthiga Selvaraju, Paritala Vikram, Kumara Thevan, and Mohammed Arifullah. "Melissopalynological Analysis of Forest Honey from North Malaysia." Journal of Tropical Resources and Sustainable Science (JTRSS) 4, no. 2 (August 13, 2021): 128–32. http://dx.doi.org/10.47253/jtrss.v4i2.621.

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Анотація:

Honey is a natural product widely used by humans due to its sweet taste and health benefits produced by bees from nectar and honey dew of various plants. To establish and increase the production of honey one must know the plants that take part in the production of honey. In this study pollen analysis of forest honey samples from northern part of Malaysia was carried out to determine the botanical sources playing role in the production of honey in that region. The pollen samples were acetolyzed and identified microscopically. Out of the three samples studied Baling sample was unifloral having Mimosa scabrella as predominant pollen while Jeli and Gerik samples are multifloral containing Tipo myrcia and Elais guineensis as major secondary pollen. Fabaceae family represented four pollen types and accounted 80% of pollen in Baling sample and 34 % in Gerik sample while completely absent in Jeli sample. These results showed the dominance of plants from Fabaceae family in honey production. All the samples analysed have Albizia falcataria, Eupatorium sp., Sparganium typha, Tilia sp. and Tipo myrcia in common indicating that these plants are present in all the three places and these results also can be used as a tool in geographical identification of North Malaysian honey from others.

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13

Ikegbunam, Nchedochukwu Clara, Nkechinyere Onyekwere Nweze, Okwong John Walter, Reginald Chukwuemeka Njokuocha, and Chiori O. C. AGWU. "Melissopalynological Characterization of Honey Samples from Southeastern, Nigeria." Turkish Journal of Agriculture - Food Science and Technology 10, no. 2 (March 2, 2022): 203–10. http://dx.doi.org/10.24925/turjaf.v10i2.203-210.4537.

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Анотація:

The identification of plants in which the bees forage is key in establishing bee farms and increasing honey production. In this study pollen analysis of honey samples from the southeastern part of Nigeria was carried out to ascertain their floral sources and ecological origin. The honey samples were acetolyzed and microscopically studied to determine the pollen types. A total of seventy-one pollen types belonging to forty-one families of plants were identified. The honey samples were dominated by pollen grains from the families of Arecaceae, Euphorbiaceae, Myrtaceae, Irvigiaceae, Fabaceae, Combretaceae/Melastomataceae, and phyllanthaceae. Some of the dominant pollen grain identified include Elaeis guineensis, Alchornea cordifolia, Hymenocardia acida, Ocimum gratissimum, Syzygium guineense, Nauclea latifolia and Afzelia africana. Out of the six samples studied Njikoka sample was monofloral having Mimosa pigra as predominant pollen while Ayamelum, Ekwusigo, Nsukka, Ezeagu, and Udenu samples are multifloral containing Elaeis guineensis, Phyllanthus sp., Piliostigma reticulatum, Irvingia sp., Alchornea cordifolia, and Lannea sp. as major secondary pollen. All the samples analyzed have Elaeis guineensis in common except Ezeagu, indicating that these plants are present in all five locations. These results can also be used as a tool in the geographical identification of Southeastern Nigeria honey from other geopolitical zones.

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14

Kirs, Evelin, Raili Pall, Kaie Martverk, and Katrin Laos. "Physicochemical and melissopalynological characterization of Estonian summer honeys." Procedia Food Science 1 (2011): 616–24. http://dx.doi.org/10.1016/j.profoo.2011.09.093.

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15

Saravanan, Mohanasundaram, Gunasekaran Mohanapriya, Ramachandra Laha, and Ramalingam Sathishkumar. "DNA barcoding detects floral origin of Indian honey samples." Genome 62, no. 5 (May 2019): 341–48. http://dx.doi.org/10.1139/gen-2018-0058.

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Анотація:

The unique medicinal and nutritional properties of honey are determined by its chemical composition. To evaluate the quality of honey, it is essential to study the surrounding vegetation where honeybees forage. In this study we used conventional melissopalynological and DNA barcoding techniques to determine the floral source of honey samples collected from different districts of the state of Mizoram, India. Pollen grains were isolated and genomic DNA was extracted from the honey samples. PCR amplification was carried out using universal barcode candidates ITS2 and rbcL to identify the plant species. Furthermore, TA cloning was carried out to screen the PCR amplicon libraries to identify the presence of multiple plant species. Results from both the melissopalynological and DNA barcoding analyses identified almost exactly the same 22 species, suggesting that both methods are suitable for analysis. However, DNA barcoding is easier and widely practiced. Hence, it can be concluded that DNA barcoding is a useful tool in determining the medicinal and commercial value of honey.

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16

ÇELEMLİ, Omur Gencay, Aslı ÖZKÖK, Çiğdem ÖZENİRLER, Nazlı MAYDA, Golshan ZARE, and Kadriye SORKUN. "HIGHLIGHTING THE MELISSOPALYNOLOGICAL AND PHYSICOCHEMICAL CHARACTERISTICS OF AYDER-RİZE (TURKEY)." Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering 30, no. 2 (December 31, 2021): 119–33. http://dx.doi.org/10.53447/communc.906788.

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The aim of this research is characterizing the honey produced in Ayder/Rize-Turkey. In this context 20 honey samples were collected from Ayder in 2018. The melissopalynological and physicochemical analysis of the honey samples were done by this research. According to the melissopalynological analysis 40 taxa belong to the 22 plant families were identified as botanical sources of the investigated honey samples. Nine of the investigated samples (sample no 1-9) were evaluated as monofloral and the others as multifloral honeys. As a result of melissopalynological analysis, while pollen belong to the Castanea sativa taxon were found in dominant ratios in some of the investigated samples, the pollen belongs to the Castanea sativa, Trifolium repens, Lotus corniculatus, Coronilla orientalis taxa were found as secondary in some other honey samples. Total pollen number in 10 gram honey (TPN 10) values of the samples were found between 7 732 and 167 147 by melissopalynological analysis. The first step of the physicochemical analsyis was the moisture analysis and the values found between 15,8% and 18,8% (mean value:7,01±0,98 %). The fructose/glucose analysis were done by High Performance Liquid Chromatography (HPLC) and the values for fructose found as: 26,43-35,57g/100g (mean: 31,39±2,41 g/100g), glucose values as; 20,11-30,58 g/100g (mean: 26,83±2,16 g/100g). Also fructose/glucose ratio was found as 1,03-1,34 (mean: 1,17±0,10). Hydroxymethylfurfural (HMF) and total phenolic acids analysis were done also by HPLC. The results for HMF analysis is between 0,7- 11,31 ppm (mean: 3,8±2,6 ppm ), for total phenolic acids the value are found between 88,92±0,04 mgGAE/kg - 196,17±0,10 mgGAE/kg (mean: 121,98±0,1 mgGAE/kg). As a step of chemical analysis; the volatile compounds of the honey samples were determined by Gas Chromatography and Mass Spectrometry (GC-MS). The compounds belong to the aldehydes, aliphatic acids and esters, alcohols, hydrocarbons, carboxylic acids and their esters, ketones, terpenes, fatty acids and their esters groups were found as a result of GC-MS analysis. The compounds belong to the carboxylic acids and their esters, fatty acids and their esters identified in higher ratios compare to the other compounds. Since, there is little detailed published information about the quality and properties of Ayder-Rize honey, the current study aims to characterize the honey belong to the this region.

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17

Dixit, Swati, S. K. Basumatary, H. Singh, and S. K. Bera. "Melissopalynological studies of western part of Almora District, Uttarakhand." Journal of Palaeosciences 62, no. (1-2) (December 31, 2013): 39–46. http://dx.doi.org/10.54991/jop.2013.335.

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Анотація:

The study of pollen contents has been carried out from the honey samples procured from the western zone of Almora District, Uttarakhand. A total of ten honey samples were collected of which four were unifloral and two bifloral. Sapindus rarak, Brassica campestris, Salmalia malabaricum, Caesalpinia pulcherrima, Coriandrum sativum, Syzygium cumini and Mimosa pudica are the dominant pollen types observed in the samples. Besides, the other reliable nectar sources were also identified from the study area. The numerous pollen types and their diversity showed that bees travel considerable distance for collecting nectar for honey production.

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18

Bandeira, Michele da Silva Ferreira, and Jaílson Santos de Novais. "Melissopalynological characterization of honeys from the Discovery Coast, Brazil." Palynology 44, no. 3 (September 5, 2019): 539–50. http://dx.doi.org/10.1080/01916122.2019.1631897.

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19

Panseri, Sara, Alessandra Manzo, Luca Maria Chiesa, and Annamaria Giorgi. "Melissopalynological and Volatile Compounds Analysis of Buckwheat Honey from Different Geographical Origins and Their Role in Botanical Determination." Journal of Chemistry 2013 (2013): 1–11. http://dx.doi.org/10.1155/2013/904202.

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Анотація:

Volatile organic compounds (VOCs) have been proposed as one of the main factors for differentiating honeys from different botanical/floral origins. In this work, we investigated the volatile profile of honeys, commercially labeled as buckwheat honeys, from the Alps and its relationship with melissopalynological investigation. The results showed that buckwheat honey samples that contained, to different extents, buckwheat pollen grains on melissopalynological analyses showed similar VOCs profiles, distinguishing them from the other honey floral types analyzed. Among VOCs identified, 3-methylbutanal, butanoic acid, pentanoic acid, and isovaleric acid were considerably greater in the buckwheat honey samples from the Alps. Other compounds were identified only in the honeys containing buckwheat pollen grains such as 3-methyl-2-buten-1-ol, 2-butanone, 2-hydroxy-3-pentanone, 4-methylpentanoic acid, 4-pentanoic acid, butanal, 2-methylbutanal, pentanal, dihydro-2-methyl-3(2H)-furanone, 5-methylfurfural, andcis-linalool oxide. These compounds give to buckwheat honey its characteristic aromatic and organoleptic properties and may be considered interesting as potential “variety markers” for botanical determination.

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20

Hegazi, Ahmed G., Fayez M. Al Guthami, Mohamed F. A. Ramadan, Ahmed F. M. Al Gethami, A. Morrie Craig, and Salud Serrano. "Characterization of Sidr (Ziziphus spp.) Honey from Different Geographical Origins." Applied Sciences 12, no. 18 (September 16, 2022): 9295. http://dx.doi.org/10.3390/app12189295.

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Анотація:

The current investigation was conducted to assess the melissopalynological, physicochemical, and biochemical properties, antimicrobial and antioxidant activities as well as total phenolic and total flavonoid contents of 794 Sidr honey samples collected from the Saudi market that had been imported from 12 different countries. Testing Sidr honey from different countries showed different levels of growth suppression observed against five drug resistant bacterial strains. The pathogenic strains were Staphylococcus aureus, Streptococcus mutans, Klebsiella pneumoniae, Escherichia coli and Pseudomonas aeruginosa. The antimicrobial activity showed growth suppression levels which varied according to the origin of the honey. The comparative study of Sidr honeys revealed a strong correlation between total polyphenol and flavonoid contents and significant radical scavenging activities in particular Egyptian and Saudi Arabian honeys. The melissopalynological and physicochemical properties of different Sidr honeys complied with the recommendations of the WHO Codex Alimentarius, the European Union standards for honey quality, and the Gulf Technical Regulation on honey (GSO 147:2008-Standards Store-GCC Standardization Organization). It was concluded that Sidr honey from different geographical areas has the capacity to suppress the growth of pathogenic bacteria and perform significant radical scavenging activities.

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21

Javůrková, Zdeňka, Matej Pospiech, Simona Ljasovská, Pavel Hrabec, and Bohuslava Tremlová. "Numerical methods and image processing techniques for melissopalynological honey analysis." Potravinarstvo Slovak Journal of Food Sciences 15 (January 28, 2021): 58–65. http://dx.doi.org/10.5219/1517.

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Анотація:

Pollen analysis is a method used for verification of the botanical and geographical honey origin. Currently, much effort is being made to introduce automated systems with the use of image analysis programs. The automatic analysis is impeded by the insufficient depth of field of objects when using a light microscope, however, this can be avoided by using image reconstruction from images obtained from different focal planes. In this method, testing was performed on the normal focus (NF) and extended-depth-of-focus (EDF) images. These two methods were compared and statistically evaluated. The number of pollen grains and selected morphometric characteristics were compared. For EDF images, a higher number of pollen grains was obtained for the analysis, and except for the length/width ratio, a statistically significant difference was observed in the characteristics of pollen grains between the compared NF and EDF methods.

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22

Costa, María Cristina, Víctor Ariel Vergara-Roig, and Silvia Clara Kivatinitz. "A melissopalynological study of artisanal honey produced in Catamarca (Argentina)." Grana 52, no. 3 (August 25, 2013): 229–37. http://dx.doi.org/10.1080/00173134.2013.819525.

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23

Jaafar, Katherine, Janay Haidar, Sawsan Kuraydiyyah, Tarek Ghaddar, Khouzama Knio, Baraem Ismail, and Imad Toufeili. "Physicochemical, melissopalynological and antioxidant properties of artisanal honeys from Lebanon." Journal of Food Science and Technology 54, no. 8 (May 23, 2017): 2296–305. http://dx.doi.org/10.1007/s13197-017-2667-8.

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24

Sahney, Manju, Snehlata Rahi, Ajay Kumar, and Roma Jaiswal. "Melissopalynological studies on winter honeys from Allahabad, Uttar Pradesh, India." Palynology 42, no. 4 (February 1, 2018): 540–52. http://dx.doi.org/10.1080/01916122.2017.1418445.

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25

Dimou, Maria. "Comparison of phenological, aerobiological and melissopalynological patterns of Olea europaea." Apidologie 43, no. 1 (October 14, 2011): 103–12. http://dx.doi.org/10.1007/s13592-011-0100-2.

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26

Villalpando-Aguilar, José Luis, Víctor Hugo Quej-Chi, Itzel López-Rosas, William Cetzal-Ix, Víctor Ángel Aquino-Luna, Fulgencio Alatorre-Cobos, and Jesús Froylán Martínez-Puc. "Pollen Types Reveal Floral Diversity in Natural Honeys from Campeche, Mexico." Diversity 14, no. 9 (September 9, 2022): 740. http://dx.doi.org/10.3390/d14090740.

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Анотація:

The Yucatan Peninsula, located in southern Mexico, is a central honey-producing region with extraordinary biodiversity of melliferous plants. Approximately 900 plant species have been described as being a source of nectar and pollen for bees and other pollinators. They provide ecosystem services that help to keep plant biodiversity high and mitigate the effects of climate change. This study aimed to reveal the diversity of the pollen content in honey through a melissopalynological analysis of 22 honey samples collected in February–August 2021 from the north-central area of Campeche, Mexico. The extraction of pollen from the honey was carried out using standard methods for melissopalynological analysis. The honeys were classified by botanical origin to determine their floral sources and a diverse spectrum of 19 pollen types from 13 families was identified. Only eight were predominant: Milleria quinqueflora, Gymnopodium floribundum, Terminalia buceras, Amaranthus spinosus, Zea mays, Talisia floresii, Guazuma ulmifolia, and Croton icche. Our research shows the high quality of the honey analyzed and highlights the diversity and critical role of local melliferous flora and crops in beekeeping development in southern Mexico. The results in this study are useful for confirming the botanical origins of honey, generating information for designing nature conservation and agroecosystem management strategies, and increasing the knowledge of beekeepers in Campeche, Mexico.

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27

Pauliuc, Daniela, Florina Dranca, and Mircea Oroian. "Antioxidant Activity, Total Phenolic Content, Individual Phenolics and Physicochemical Parameters Suitability for Romanian Honey Authentication." Foods 9, no. 3 (March 8, 2020): 306. http://dx.doi.org/10.3390/foods9030306.

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Анотація:

The present study aimed to evaluate the physicochemical characteristics of honey (raspberry, mint, rape, sunflower, thyme and polyfloral) produced in Romania. The honey samples were from the 2017 to 2018 harvest and were subjected to melissopalynological analysis, alongside the determination of the following physicochemical parameters: moisture content, pH, free acidity, electrical conductivity (EC), hydroxymethylfurfural (HMF) content, color, total polyphenols content (TPC), flavonoids content (FC), DPPH radical scavenging activity, phenolic acids, flavonols, sugars and organic acids in order to evaluate the usefulness of this parameters for the classification of honey according to botanical origin. The results of the melissopalynological analysis revealed that five types of honey samples had a percentage of pollen grains above the minimum of 45%, which was required in order to classify the samples as monofloral honey. The total polyphenols content reached the maximum value in the case of dark honey such as mint honey, followed by raspberry, thyme and polifloral honey. Fructose, glucose, maltose, sucrose, turanose, trehalose, melesitose, and raffinose were identified and quantified in all samples. Gluconic acid was the main organic acid in the composition of all honey samples. Principal component analysis (PCA) confirmed the possibility of the botanical authentication of honey based on these physicochemical parameters.

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28

Pauliuc, Daniela, Florina Dranca, and Mircea Oroian. "Raspberry, Rape, Thyme, Sunflower and Mint Honeys Authentication Using Voltammetric Tongue." Sensors 20, no. 9 (April 30, 2020): 2565. http://dx.doi.org/10.3390/s20092565.

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The aim of this study was to authenticate five types of Romanian honey (raspberry, rape, thyme, sunflower and mint) using a voltammetric tongue (VE tongue) technique. For the electronic tongue system, six electrodes (silver, gold, platinum, glass, zinc oxide and titanium dioxide) were used. The results of the melissopalynological analysis were supplemented by the data obtained with the electronic voltammetric tongue system. The results were interpreted by means of principal component analysis (PCA) and linear discriminant analysis (LDA). In this way, the usefulness of the working electrodes was compared for determining the botanical origin of the honey samples. The electrodes of titanium dioxide, zinc oxide, and silver were more useful, as the results obtained with these electrodes showed that it was achieved a better classification of honey according to its botanical origin. The comparison of the results of the electronic voltammetric tongue technique with those obtained by melissopalynological analysis showed that the technique was able to accurately classify 92.7% of the original grouped cases. The similarity of results confirmed the ability of the electronic voltammetric tongue technique to perform a rapid characterization of honey samples, which complements its advantages of being an easy-to-use and cheap method of analysis.

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Balkanska, Ralitsa, Katerina Stefanova, Radostina Stoikova-Grigorova, Dessislava Gerginova, Svetlana Simova, and Ivan Atanassov. "Assessment of the botanical origin of Bulgarian honey samples using melissopalynological, DNA barcoding and NMR analyses." Botanica Serbica 47, no. 1 (2023): 75–85. http://dx.doi.org/10.2298/botserb2301075b.

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Polyfloral honey samples from Bulgaria were subject to parallel analyses of their botanical origin and composition using traditional melissopalynology, DNA barcoding based on the plastid rbcL gene and NMR analysis. The obtained datasets were compared with each other to evaluate the information capacity of the applied experimental methods. The results from the melissopalynological and DNA-barcoding studies demonstrated a significantly higher resolution of the latter, revealing the presence of pollen from a total of 17 plant families, 21 plant genera and 5 plant species in comparison to pollen from only 7 plant families, 3 plant genera and 4 plant species identified by melissopalynology. The higher resolution of DNA barcoding allows a more detailed characterisation of the diet and foraging preferences of honey bees, including foraging on plant species growing in lower abundance in the area. The comparison of the quantitative data on floral honey composition for several plant genera and species reveals significant differences between the relative abundance of the pollen grains estimated by melissopalynological analysis and the relative abundance of rbcL clones in rbcL libraries determined after DNA barcoding. All three applied methods confirm the polyfloral botanical origin of the analysed samples and support routine NMR use for the assessment of the floral origin of honey.

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Li, X., J. G. Prebble, P. J. de Lange, J. I. Raine, and L. Newstrom-Lloyd. "Discrimination of pollen of New Zealand mānuka (Leptospermum scoparium agg.) and kānuka (Kunzea spp.) (Myrtaceae)." PLOS ONE 17, no. 6 (June 3, 2022): e0269361. http://dx.doi.org/10.1371/journal.pone.0269361.

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The very similar appearance of pollen of the New Zealand Myrtaceous taxa Leptospermum scoparium s.l. (mānuka) and Kunzea spp. (kānuka) has led palynologists to combine them in paleoecological and melissopalynological studies. This is unfortunate, as differentiation of these taxa would improve understanding of past ecological change and has potential to add value to the New Zealand honey industry, where mānuka honey attracts a premium price. Here, we examine in detail the pollen morphology of the 10 Kunzea species and a number of Leptospermum scoparium morphotypes collected from around New Zealand, using light microscopy, SEM, and Classifynder (an automated palynology system). Our results suggest that at a generic level the New Zealand Leptospermum and Kunzea pollen can be readily differentiated, but the differences between pollen from the morphotypes of Leptospermum or between the species of Kunzea are less discernible. While size is a determinant factor–equatorial diameter of Leptospermum scoparium pollen is 19.08 ± 1.28 μm, compared to 16.30 ± 0.95 μm for Kunzea spp.–other criteria such as surface texture and shape characteristics are also diagnostic. A support vector machine set up to differentiate Leptospermum from Kunzea pollen using images captured by the Classifynder system had a prediction accuracy of ~95%. This study is a step towards future melissopalynological differentiation of mānuka honey using automated pollen image capture and classification approaches.

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Hegazi, Ahmed G., Fayez M. Al Guthami, Mohamed F. A. Ramadan, Ahmed F. M. Al Gethami, A. Morrie Craig, Hesham R. El-Seedi, Inmaculada Rodríguez, and Salud Serrano. "The Bioactive Value of Tamarix gallica Honey from Different Geographical Origins." Insects 14, no. 4 (March 27, 2023): 319. http://dx.doi.org/10.3390/insects14040319.

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This study was conducted to assess the bioactive value of Tamarix gallica honey samples collected from three countries. In total, 150 Tamarix gallica honey samples from Saudi Arabia (50), Libya (50), and Egypt (50) were collected and compared, based on the results of the melissopalynological analysis, their physicochemical attributes, antioxidant and antimicrobial activities, and biochemical properties, together with their total phenolic and total flavonoid contents. Depending on the geographical origin, we observed different levels of growth suppression for six resistant bacterial strains. The pathogenic microorganisms tested in this study were Staphylococcus aureus, Streptococcus mutans, Klebsiella pneumoniae, Escherichia coli, Proteus vulgaris, and Pseudomonas aeruginosa. There was a strong correlation between the polyphenol and flavonoid contents, as well as significant (p < 0.05) radical scavenging activities. The melissopalynological analysis and physicochemical properties complied with the recommendation of the Gulf and Egyptian Technical Regulations on honey, as well as the Codex Alimentarius of the World Health Organization and the European Union Normative related to honey quality. It was concluded that Tamarix gallica honey from the three countries has the capacity to suppress pathogenic bacterial growth and has significant radical scavenging activities. Moreover, these findings suggest that Tamarix gallica honey may be considered as an interesting source of antimicrobial compounds and antioxidants for therapeutical and nutraceutical industries or for food manufacturers.

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Bryś, Maciej S., Magdalena Kunat, Ernest Stawiarz, and Aneta A. Ptaszyńska. "PHYSICOCHEMICAL CHARACTERISTICS AND MELISSOPALYNOLOGICAL ANALYSIS OF COMMERCIAL BUCKWHEAT HONEYS FROM POLAND." Journal of Experimental Biology and Agricultural Sciences 9, no. 2 (April 25, 2021): 200–205. http://dx.doi.org/10.18006/2021.9(2).200.205.

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Buckwheat honey is widely consumed by consumers due to its numerous health-promoting properties. Characteristically it is dark, tea-like in colour, sharp, tickly, and sweet in flavour, and has smelled of buckwheat flowers. In the current study, various commercial honey samples were examined to test the quality of buckwheat honey samples available in the market. The research materials were comprised of 15 samples of honeys from 4 voivodships, among these, 5 samples were collected from the Świętokrzyskie Voivodship, 4 from the Lesser Poland Voivodeship, 4 from the Lubelskie Voivodship, and reset 2 samples from the Podkarpackie Voivodship. Melissopalynological analyses of investigated honeys’ samples revealed that all samples had at least 45% of Fagopyrym pollen content, which means that researched honeys complied with the standards of the International Commission for Bee Botany (ICBB) for buckwheat honey. Honeys’ samples had an average water content of 15.3% (σ= 1.24), and electrical conductivity at 0.37 mS*cm-1. Therefore, all beekeepers correctly marked their honey type as buckwheat honey simply using the organoleptic properties of their honeys and observing their bees collecting pollen and honeydew.

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Goncharov, B. I., and P. P. Snegur. "USING TRIFLOUROACETIC ACID IN ACETOLYSIS OF POLLEN GRAINS IN MELISSOPALYNOLOGICAL ANALYSIS." Bulletin оf Kamchatka State Technical University, no. 57 (2021): 96–100. http://dx.doi.org/10.17217/2079-0333-2021-57-96-100.

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Aronne, Giovanna, Veronica De Micco, and Mario R. Guarracino. "Application of Support Vector Machines to Melissopalynological Data for Honey Classification." International Journal of Agricultural and Environmental Information Systems 1, no. 2 (July 2010): 85–94. http://dx.doi.org/10.4018/jaeis.2010070105.

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In this paper, the authors address the problem of the discrimination of geographical origin and the selection of marker species of honeys using Support Vector Machines and z-scores. The methodology is based on the elaboration of palynological data with statistical learning methodologies. This innovative solution provides a simple yet powerful tool to detect the origin of honey samples. In case of honeys from Sorrento Peninsula, the discrimination from other Italian honeys is obtained with high accuracy.

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Jamwal, Radhika, and V. K. Mattu. "Melissopalynological investigations on summer honey samples from Mandi District, Himachal Pradesh." Indian Journal of Entomology 81, no. 1 (2019): 90. http://dx.doi.org/10.5958/0974-8172.2019.00024.5.

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Yang, Yin, Marie-José Battesti, Jean Costa, and Julien Paolini. "Melissopalynological and Volatile Analysis of Honeys from Corsican Arbutus unedo Habitat." Natural Product Communications 9, no. 10 (October 2014): 1934578X1400901. http://dx.doi.org/10.1177/1934578x1400901030.

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Thirty Corsican “autumn maquis” honeys were characterized by the typical combination of autumnal taxa: Arbutus unedo, Hedera helix, Smilax aspera, Rosmarinus officinalis, and two Asteraceae pollen forms. Corsican origin was characterized by the diversity of the taxa's biogeographical origins and significant presence of Castanea sativa and Quercus sp. Volatile fractions of “autumn maquis” honeys were dominated by isophorone and 3,4,5-trimethylphenol. The latter is reported in A. unedo honey for the first time. Otherwise, both A. unedo flower and “autumn maquis” honeys exhibited high contents of isophorone derivatives. H. helix honey exhibited phenylacetaldehyde, benzyl nitrile, 3-hydroxy-4-phenylbutan-2-one and nonanal as major compounds, which were scarcely represented in the studied “autumn maquis” honey samples.

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Thakodee, Thanyalak, Sureerat Deowanish, and Kiattisak Duangmal. "Melissopalynological analysis of stingless bee (Tetragonula pagdeni) honey in Eastern Thailand." Journal of Asia-Pacific Entomology 21, no. 2 (June 2018): 620–30. http://dx.doi.org/10.1016/j.aspen.2018.04.003.

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38

Oyeyemi, Sunday Dele, Sylvester Onoriode Obigba, and Oluseyi Oriyomi Murele. "Melissopalynological and Physicochemical Analysis of Honey Samples from Ekiti, Southwestern Nigeria." Singapore Journal of Scientific Research 13, no. 1 (July 6, 2023): 48–59. http://dx.doi.org/10.3923/sjsr.2023.48.59.

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39

Ben Amor, Safia, Scherazad Mekious, Leila Allal Benfekih, Magda H. Abdellattif, Walid Boussebaa, Faisal A. Almalki, Taibi Ben Hadda, and Sarkar M. A. Kawsar. "Phytochemical Characterization and Bioactivity of Different Honey Samples Collected in the Pre-Saharan Region in Algeria." Life 12, no. 7 (June 21, 2022): 927. http://dx.doi.org/10.3390/life12070927.

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Despite the challenging conditions in the pre-Saharan areas of Algeria, such as weak plant cover and a harsh climate, beekeeping is being developed and spread. In the present work, honey samples collected from ten locations in the El Oued region were examined during the spring of 2021. A melissopalynological analysis was carried out, followed by a floristic investigation. The 10 honey samples were also investigated for their physicochemical properties and antioxidant and antibacterial activity against five strains: Escherichia coli, Staphylococcus aureus, Bacillus subtilus, Listeria innocua, and Micrococcus luteus. The floristic analysis found 65 species belonging to 33 botanical families, with a dominance of the Asteraceae family accounting for 18.461% of the total. The melissopalynological study revealed only one monofloral honey (Ziziphus lotus), whereas the nine others were multi-floral. The honey’s color changed from light to dark amber, and most tested honey was of high quality, fulfilling international criteria. The total phenol and flavonoid contents varied considerably amongst the various honey samples. Furthermore, LC-MS-MS phenolic profile analysis identified the presence of 20 chemicals, of which only three phenols were found in all honey types. Antioxidant capacity analyzed with FRAP test and antiradical activities against DPPH differed from one honey sample to another. Moreover, a significant correlation was recorded between the antioxidant activity, honey’s color, polyphenol, and flavonoid contents. The S. aureus strain was the most sensitive regarding honey antibacterial activity, while M. luteus and B. subtilis strains were only moderately sensitive.

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40

Goncharov, B. I., and M. V. Lebedko. "THE POSSIBILITY OF USING TRICHLOROACETIC ACID FOR ACETOLYSIS OF POLLEN GRAINS IN MELISSOPALYNOLOGICAL ANALYSIS." Bulletin of Kamchatka State Technical University, no. 66 (2024): 80–87. http://dx.doi.org/10.17217/2079-0333-2023-66-80-87.

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The possibility of using trichloroacetic acid instead of the classical mixture of acetic anhydride with sulfuric acid for acetolysis of pollen grains in the melissopalynological analysis of honey is demonstrated. This substance in comparison with other derivatives of acetic acid allows holding the sample in a boiling water bath that improves the result of acetolysis of pollen grains. The possibility of using various solvents for transferring acetolyzed pollen grains to glass slides for further microscopy was also investigated. The optimal reagents and parameters for this analysis were studied and identified during the experiments.

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41

Selvaraju, Kirthiga, Paritala Vikram, Jan Mei Soon, Kumara Thevan Krishnan, and Arifullah Mohammed. "Melissopalynological, physicochemical and antioxidant properties of honey from West Coast of Malaysia." Journal of Food Science and Technology 56, no. 5 (April 3, 2019): 2508–21. http://dx.doi.org/10.1007/s13197-019-03728-3.

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Yang, Yin, Marie-José Battesti, Nassim Djabou, Alain Muselli, Julien Paolini, Pierre Tomi, and Jean Costa. "Melissopalynological origin determination and volatile composition analysis of Corsican “chestnut grove” honeys." Food Chemistry 132, no. 4 (June 2012): 2144–54. http://dx.doi.org/10.1016/j.foodchem.2011.07.075.

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43

Macukanovic-Jocic, Marina, Dragana Rancic, and Zora Dajic-Stevanovic. "Palynomorphological study of primrose (Primula vulgaris Huds.) grown in natural reserve Obedska bara (Serbia)." Journal of Agricultural Sciences, Belgrade 55, no. 3 (2010): 227–34. http://dx.doi.org/10.2298/jas1003227m.

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The pollen morphology of primrose (Primula vulgaris, fam. Primulaceae) has been investigated using light and scanning electron microscopy to contribute to melissopalynological studies of honeys originating from the native apiflora. Palynomorphological investigation included the examination of pollen symmetry, polarity, ornamentation, aperturation, shape and size. The pollen grains are isopolar, radially symmetric and shed as monads. The exine ornamentation is reticulate. Analysis of pollen morphometric characteristics revealed that grains are small to medium size and prolate in shape. Given the aperturation, the number of colpi was mostly variable among individuals with a different type of flower ranging from 6 to 9.

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Ghorab, Asma, María Shantal Rodríguez-Flores, Rifka Nakib, Olga Escuredo, Latifa Haderbache, Farid Bekdouche, and María Carmen Seijo. "Sensorial, Melissopalynological and Physico-Chemical Characteristics of Honey from Babors Kabylia’s Region (Algeria)." Foods 10, no. 2 (January 22, 2021): 225. http://dx.doi.org/10.3390/foods10020225.

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This study aimed to characterize the honeys of Babors Kabylia through sensory, melissopalynological and physico-chemical parameters. Thirty samples of honey produced in this region were collected over a period of two years and analyzed. All the samples presented physico-chemical parameters in conformity with legislation on honey quality, with few exceptions, linked mainly to beekeeping management. The pollen spectrum revealed a great diversity with 96 pollen types. The main pollen types were spontaneous species as Fabaceae (Hedysarum, Trifolium, Genisteae plants), Asteraceae plants, Ericaceae (Erica arborea L.) or Myrtus and Pistacia. The sensory properties of samples showed a high tendency to crystallization, the colors were from white to brown, but most of them had gold color. Smell and odor corresponded mainly to vegetal and fruity families and in taste perceptions besides sweetness highlighted sourness and saltiness notes. Seventeen samples were polyfloral, one was from honeydew and twelve were monofloral from heather, genista plants, sulla, blackberry or Asteraceae. Heather and the honeydew samples showed the darkest color, the highest electrical conductivity and phenol and flavonoid content. A statistical analysis based on the most representative pollen types, sensory properties and some physico-chemical components allowed the differentiation of honey samples in terms of botanical origin.

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Prđun, Saša, Dario Kremer, Dragan Bubalo, and Lidija Svečnjak. "Physico-chemical, melissopalynological and sensory characteristics of Satsuma mandarin honey (Citrus unshiu Marc.)." Journal of Central European Agriculture 21, no. 2 (2020): 256–67. http://dx.doi.org/10.5513/jcea01/21.2.2787.

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46

Barth, Ortrud Monika, and Cynthia Fernandes Pinto Da Luz. "Melissopalynological data obtained from a mangrove area near to Rio de Janeiro, Brazil." Journal of Apicultural Research 37, no. 3 (January 1998): 155–63. http://dx.doi.org/10.1080/00218839.1998.11100967.

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47

Silva, Tania Maria Sarmento, Francyana Pereira dos Santos, Adriana Evangelista-Rodrigues, Eva Mônica Sarmento da Silva, Gerlania Sarmento da Silva, Jaílson Santos de Novais, Francisco de Assis Ribeiro dos Santos, and Celso Amorim Camara. "Phenolic compounds, melissopalynological, physicochemical analysis and antioxidant activity of jandaíra (Melipona subnitida) honey." Journal of Food Composition and Analysis 29, no. 1 (February 2013): 10–18. http://dx.doi.org/10.1016/j.jfca.2012.08.010.

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48

Mazai, Husaina M., Sani Ibrahim, Aliyu Muhammad, and Abdulmumin Z. Abubakar. "Melissopalynological, Physicochemical, Antimicrobial and Microbiological Attributes of Commercial Honeys from North West Nigeria." Current Nutrition & Food Science 16, no. 6 (July 15, 2020): 972–87. http://dx.doi.org/10.2174/1573401315666190904143858.

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Background: Honey is used for nutritional, medicinal and industrial purposes and maintenance of the requisite quality is of great importance. Objective: This study distinctively aimed to portray twelve randomly selected profitable honeys accessible in the North West Nigerian market with regards to floral nectar origin, physicochemical factors, antimicrobial activity and microbial safety evaluations. Methods: Twelve (12) samples of honey sourced from diverse places in the Northwest of Nigeria were analyzed for their pollen spectra, physicochemical parameters, antimicrobial activity and microbiological safety. Results: Dominant pollen types from the analysis were Borassus (72%), Piptadenia africana (39%), Lophira (91%) and Combretaceae (91%) for Sokoto, Jigawa, Kaduna and Katsina States honey samples, respectively. The physicochemical analysis of the samples revealed that pH was in range from 3.32 to 4.25, moisture content varied from 16.37% to 18.70%, ash content 0.23% to 0.93% and free acidity 6.83meq/Kg to 32.00meq/kg, electrical conductivity ranged from 0.13mS/cm to 1.40mS/cm, reducing sugars was 42.85% to 49.30%, sucrose content was 0.74% to 6.75%, Hydroxymethylfurfural value was 11.0mg/Kg to 80.20mg/Kg, diastase level ranged from 7.23 to 43.5 and Proline content ranged from 104.67mg/Kg to 666.67mg/Kg. The antimicrobial evaluation revealed significant zone of inhibition to E. coli, ranging from 19-38 mm at concentrations (12.5-100mg/mL) tested as compared to all other test organisms. Conclusion: The fallouts of microbiological investigation of the samples revealed mould and bacteria, with counts less than 102CFU/g. The findings showed that the attributes of the tested honey samples maintained criteria of international standards.

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Upadhyay, Debasis, Swapan Bhattacharya, David K. Ferguson, and Subir Bera. "Prospects of Apicultural Entrepreneurship in Coastal Districts of Eastern India: A Melissopalynological Evaluation." PLoS ONE 9, no. 4 (April 16, 2014): e94572. http://dx.doi.org/10.1371/journal.pone.0094572.

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50

Ketfi, Louiza, Radia Draiaia, Asma Necib, and Nadia Mohamadi. "Physicochemical and Melissopalynological Study of Some Honey Samples from the Algerian East Region." Turkish Journal of Agriculture - Food Science and Technology 11, no. 4 (April 26, 2023): 780–84. http://dx.doi.org/10.24925/turjaf.v11i4.780-784.5941.

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Анотація:

The quantitative and qualitative analysis of honey components is the object of this study. These analyses are considered a physicochemical and melissopalynological tool that allows the study of some characteristics of honey. The analysis of ten (10) samples of Algerian honey is carried out to determine the pH, water content, electrical conductivity, ash content, acidity, hydroxymethylfurfural content, proteins, and sugar content. According to the obtained results, the physico-chemical parameters of the studied honeys comply with the European and international standards. The pollen spectrum indicated the presence of 23 plant families, mainly Fabaceae, encompassing a total of 65 honey species visited by foragers. Of the 10 honeys sampled and analyzed, six were monofloral, with the remainder showing no apparent dominance of any honey type. The most frequent plant species were eucalyptus (Eucalyptus sp.), Italian sainfoin (Hedysarum coronarium), and chickweed (Lathyrus sp.). Furthermore, the results obtained indicate that all honey (monofloral and polyfloral) showed a high degree of variability in the number of pollen grains.

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