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Добірка наукової літератури з теми "Matrice MALDI"

Автор: Grafiati

Опубліковано: 22 грудня 2023

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Статті в журналах з теми "Matrice MALDI"

Rubakhin, Stanislav S., and Jonathan V. Sweedler. "Transparent triethylamine-containing MALDI matrices." Israel Journal of Chemistry 47, no. 2 (December 2007): 185–93. http://dx.doi.org/10.1560/ijc.47.2.185.

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Meier, Michael A. R., Nico Adams, and Ulrich S. Schubert. "Statistical Approach To Understand MALDI-TOFMS Matrices: Discovery and Evaluation of New MALDI Matrices." Analytical Chemistry 79, no. 3 (February 2007): 863–69. http://dx.doi.org/10.1021/ac061173v.

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HAZAMA, Hisanao, and Kunio AWAZU. "Matrices for MALDI Using an Infrared Laser." Journal of the Mass Spectrometry Society of Japan 64, no. 5 (2016): 183–86. http://dx.doi.org/10.5702/massspec.s16-38.

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FUKUYAMA, Yuko. "MALDI MS Analysis Using Liquid Matrices." Journal of the Mass Spectrometry Society of Japan 64, no. 5 (2016): 175–78. http://dx.doi.org/10.5702/massspec.s16-36.

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Fresnais, Margaux, Esra Yildirim, Seda Karabulut, Dirk Jäger, Inka Zörnig, Julia Benzel, Kristian W. Pajtler, et al. "Rapid MALDI-MS Assays for Drug Quantification in Biological Matrices: Lessons Learned, New Developments, and Future Perspectives." Molecules 26, no. 5 (February 26, 2021): 1281. http://dx.doi.org/10.3390/molecules26051281.

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Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has rarely been used in the field of therapeutic drug monitoring, partly because of the complexity of the ionization processes between the compounds to be quantified and the many MALDI matrices available. The development of a viable MALDI-MS method that meets regulatory guidelines for bioanalytical method validation requires prior knowledge of the suitability of (i) the MALDI matrix with the analyte class and properties for ionization, (ii) the crystallization properties of the MALDI matrix with automation features, and (iii) the MS instrumentation used to achieve sensitive and specific measurements in order to determine low pharmacological drug concentrations in biological matrices. In the present hybrid article/white paper, we review the developments required for the establishment of MALDI-MS assays for the quantification of drugs in tissues and plasma, illustrated with concrete results for the different steps. We summarize the necessary parameters that need to be controlled for the successful development of fully validated MALDI-MS methods according to regulatory authorities, as well as currently unsolved problems and promising ways to address them. Finally, we propose an expert opinion on future perspectives and needs in order to establish MALDI-MS as a universal method for therapeutic drug monitoring.

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Lin, Chih-Hao, Chuping Lee, Yu-Cheng Wu, and I.-Chung Lu. "New Strategy to Preserve Phosphate by Ionic Liquid Matrices in Matrix-Assisted Laser Desorption/Ionization: A Case of Adenosine Nucleotides." Molecules 25, no. 5 (March 8, 2020): 1217. http://dx.doi.org/10.3390/molecules25051217.

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Adenosine -5′-triphosphate (ATP) plays a valuable role in metabolic activity to produce adequate energy in a biosystem. A high ATP/AMP ratio has a correlation with diabetes that induces suppression of AMP-activated protein kinase (AMPK). Matrix-assisted laser desorption/ionization (MALDI)–mass spectrometry (MS) has outstanding potential in determining the ratio of several types of adenosine phosphates in a sample to rapidly understand the primary energy transfer in metabolism. Although MALDI is viewed as a soft ionization technique for MS analysis, excess photon energy might crack the phosphate bonds leading to misinterpretation of the ATP level. In this work, ionic liquid matrices (ILMs) were employed to reduce fragmentation and increase the detection efficiency during the MALDI process. This study demonstrated for the first time that 2,5-dihydroxybenzoic acid pyridine (DHBP) is one of the most effective matrices for further quantitative analysis of adenosine nucleotides. This systematic screening of ILMs also enhances the fundamental understanding of MALDI.

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Kim, Seung-Woo, Sunbum Kwon, and Young-Kwan Kim. "Graphene Oxide Derivatives and Their Nanohybrid Structures for Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Small Molecules." Nanomaterials 11, no. 2 (January 22, 2021): 288. http://dx.doi.org/10.3390/nano11020288.

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Matrix-assisted laser desorption/ionization (MALDI) has been considered as one of the most powerful analytical tools for mass spectrometry (MS) analysis of large molecular weight compounds such as proteins, nucleic acids, and synthetic polymers thanks to its high sensitivity, high resolution, and compatibility with high-throughput analysis. Despite these advantages, MALDI cannot be applied to MS analysis of small molecular weight compounds (<500 Da) because of the matrix interference in low mass region. Therefore, numerous efforts have been devoted to solving this issue by using metal, semiconductor, and carbon nanomaterials for MALDI time-of-flight MS (MALDI-TOF-MS) analysis instead of organic matrices. Among those nanomaterials, graphene oxide (GO) is of particular interest considering its unique and highly tunable chemical structures composed of the segregated sp2 carbon domains surrounded by sp3 carbon matrix. Chemical modification of GO can precisely tune its physicochemical properties, and it can be readily incorporated with other functional nanomaterials. In this review, the advances of GO derivatives and their nanohybrid structures as alternatives to organic matrices are summarized to demonstrate their potential and practical aspect for MALDI-TOF-MS analysis of small molecules.

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Monopoli, Antonio, Giovanni Ventura, Andrea Aloia, Fulvio Ciriaco, Angelo Nacci, Tommaso R. I. Cataldi, and Cosima D. Calvano. "Synthesis and Investigation of Novel CHCA-Derived Matrices for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Lipids." Molecules 27, no. 8 (April 15, 2022): 2565. http://dx.doi.org/10.3390/molecules27082565.

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A significant area of study and upgrading for increasing sensitivity and general performances of matrix-assisted laser-desorption ionization (MALDI) mass spectrometry (MS) is related to matrix design. Several efforts have been made to address the challenge of low-mass-region interference-free for metabolomics analysis and specifically for lipidomics. To this aim, rationally designed matrices as 4-chloro-α-cyanocinnamic acid (ClCCA) were introduced and reported to provide enhanced analytical performances. We have taken this rational design one step further by developing and optimizing new MALDI matrices with a range of modifications on the CHCA core, involving different functionalities and substituents. Of particular interest was the understanding of the electron-withdrawing (e.g., nitro-) or donating (e.g., methoxy-) effects along with the extent of conjugation on the ionization efficiency. In the present work, ten matrices were designed on a reasonable basis, synthesized, and characterized by NMR and UV spectroscopies and laser desorption ionization. With the assistance of these putative MALDI matrices, samples containing phospholipids (PL), and neutral di-/tri-acylglycerols (DAG, TAG) were investigated using milk, fish, blood, and human plasma extracts. In comparison with CHCA and ClCCA, four of them, viz. [(2E,4E)-2-cyano-5-(4-methoxyphenyl)penta-2,4-dienoic acid] (1), [(2E,4E)-2-cyano-5-(4-nitrophenyl)penta-2,4-dienoic acid] (2), [(E)-2-cyano-3-(6-methoxynaphthalen-2-yl)acrylic acid] (6) and [(E)-2-cyano-3-(naphthalen-2-yl)acrylic acid] (7) displayed good to even excellent performances as MALDI matrices in terms of ionization capability, interference-free spectra, S/N ratio, and reproducibility. Especially compound 7 (cyano naphthyl acrylic acid, CNAA) was the election matrix for PL analysis and matrix 2 (cyano nitrophenyl dienoic acid, CNDA) for neutral lipids such as DAG and TAG in positive ion mode.

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dos Santos, Nayara A., Lindamara M. de Souza, Fernanda E. Pinto, Clebson de J. Macrino, Camila M. de Almeida, Bianca B. Merlo, Paulo R. Filgueiras, Rafael S. Ortiz, Ronaldo Mohana-Borges, and Wanderson Romão. "LDI and MALDI-FT-ICR imaging MS in Cannabis leaves: optimization and study of spatial distribution of cannabinoids." Analytical Methods 11, no. 13 (2019): 1757–64. http://dx.doi.org/10.1039/c9ay00226j.

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Weißflog, Jerrit, and Aleš Svatoš. "1,8-Di(piperidinyl)-naphthalene – rationally designed MAILD/MALDI matrix for metabolomics and imaging mass spectrometry." RSC Advances 6, no. 79 (2016): 75073–81. http://dx.doi.org/10.1039/c6ra17237g.

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Більше джерел

Дисертації з теми "Matrice MALDI"

Manuelli, Pascal. "Etude des mécanismes de la désorption : ionisation laser assistée par matrice." Metz, 1995. http://docnum.univ-lorraine.fr/public/UPV-M/Theses/1995/Manuelli.Pascal.SMZ9520.pdf.

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Ce mémoire sur les mécanismes de la désorption/ionisation laser assistée par matrice (MALDI) est le fruit de travaux réalisés au laboratoire de spectrométrie de masse et de chimie laser (LSMCL) de l'université de Metz. Cette thématique est apparue au laboratoire suite au développement de l'analyse des biomolécules par spectrométrie de masse et à l'expérience accumulée depuis 15 ans sur la désorption laser. Nous nous sommes attachés à décomposer les étapes constituant une analyse MALDI afin de mieux comprendre les mécanismes mis en jeu lors de ce type d'ionisation. Nous avons plus précisément, à l'aide de molécules modèles (nucléotides, peptides,), étudie l'interaction entre le faisceau laser et les matrices couramment utilisées. Ceci nous a permis de mettre en évidence le rôle important des petites molécules neutres (notamment le dioxyde de carbone) qui sont éjectées lors de l'expansion du nuage gazeux induit par irradiation laser. Dans ce contexte, nous avons étudié des dérivés et des complexes de la cyclodextrine. Cette partie nous a permis d'expliquer les phénomènes d'interaction entre la molécule cible et les molécules de matrice ainsi que l'influence de la longueur d'onde du laser ionisant. A l'aide de la technique MALDI, nous avons pu mettre en évidence des différences structurales et caractériser des complexes d'inclusion intacts
This report on the mechanisms of matrix assisted laser desorption ionization (MALDI) was realized in mass spectrometry laboratory at Metz'university. This research field appeared in the laboratory after the development of biomolecules analyses by mass spectrometry and the experience stored for fifteen years on "laser desorption". We decomposed the different states of a MALDI experiment for a better understanding of this ionization process. By using some model compounds (as peptides, nucleotides,. . . ) we have studied more precisely the interaction between a laser beam and the different matrices. This allow us to show the great role of small neutral molecules (e. G. Carbone dioxide,. . . ) which are ejected in the plume after the laser pulse. Then, some derivative and complexes of cyclodextrins have been studied. This part is necessary to explain first the interaction between matrix and target molecules and secondly the dependance on the laser wavelength. Thank's to the MALDI technique we can highlight both both structural differences in intact inclusion complexes characterization

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Jaber, Ali. "Matrices MALDI bithiophéniques spécifiques aux alcaloïdes : étude des mécanismes fondamentaux et applications." Thesis, Angers, 2017. http://www.theses.fr/2017ANGE0042/document.

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Mon travail de thèse a consisté à poursuivre le développement et l’application des matrices bithiophéniques spécifique aux alcaloïdes. Après l’optimisation d’un protocole efficace d’analyse, adapté à l’objectif de l’étude, la mise au point d’une méthode de dosage des alcaloïdes par MALDI dans des extraits végétaux et sans prétraitement préalable a été étudiée. Cette méthode a pu être validée en étudiant des alcaloïdes existant dans différents extraits des plantes toxiques. Ensuite, la synthèse et l’évaluation de nouveaux composés bithiophéniques ont été présentés de manière à évaluer les facteurs favorisant l’interaction avec les alcaloïdes. Ultérieurement, cinq nouvelles matrices intéressantes furent l’objet d’une étude plus détaillée. Sur la base des résultats obtenus, le dérivé fluoré F T3 s’avère le plus efficace. ll présente une meilleure sélectivité que la matrice courante CHCA vis-à-vis des alcaloïdes et plus performant pour analyser les alcaloïdes dans différents mélanges complexes tels que des extraits bruts de plantes, des insectes, et des solutions bio-actives commerciales (médicament et répulsif). À la fin, ces sont regroupés les résultats de l’étude des paramètres thermodynamiques de la matrice MT3P, ce qui permettra de proposer des hypothèses expliquant la sélectivité de la matrice bithiophéniques fonctionnalisée pour les alcaloïdes
My thesis work consisted of pursuing the development and application of bithiophenic maldi matrices specific for alkaloids. After optimization of an efficient analysis protocol adapted to the objective of the study, a method for the determination of alkaloids in vegetable extracts by MALDI was developed. This method was validated by studying many alkaloids existing in extracts of different toxic plants. Subsequently, synthesis and evaluation of novel bithiophenic compounds were presented in order to evaluate the factors favoring the interaction with alkaloids. Then, five matrices among the molecules tested and having produced interesting results are chosen and were the subject of a more detailed study. On the basis of the results obtained, the fluorinated derivative PFPT3P proves to be the most effective molecules. It has a better selectivity with respect to alkaloids than the current matrix HCCA and performs better to analyze these metabolites in different complex mixtures such as crude extracts of plants,insects and commercial bioactive solutions (drug and repellent). At the end, the results of the study of the thermodynamic parameters of MT3P matrix are grouped. This will make possible to propose hypotheses explaining the selectivity of the functionalized bithiophene matrices for alkaloids

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Lavigne, Damien. "Conception de surfaces de matrice extracellulaire analysables en spectrométrie de masse SELDI-TOF." Paris 7, 2008. http://www.theses.fr/2008PA077247.

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Notre projet a pour objectif de concevoir des surfaces de matrice extracellulaire (MEC) sur des chips analysables en spectrométrie de masse SELDI-TOF (Surface Enhanced Laser Desorption lonization Time Of Flight). La MEC est synthétisée directement par les cellules en culture sur les chips. Le décollement des cellules par un traitement permet ensuite d'obtenir une surface de MEC. Parmi les chips en aluminium commerciales, seules les surfaces de silice permettent la culture des cellules musculaires lisses (hCML) et endothéliales (HUVEC) humaines et d'obtenir de la MEC. La MEC de ces deux types cellulaires est différente comme le révèle leur analyse au SELDI-TOF. Néanmoins, l'opacité des chips en aluminium gêne l'observation des cellules. Nous sommes les premiers à utiliser des chips transparentes en polyméthylpentèn< (PMP) au SELDI-TOF. Comme les cellules ne peuvent être cultivées directement sur ces chips, nous avons déterminé des conditions de fonctionnalisation au plasma permettant la culture des hCML. Des améliorations sont maintenant nécessaires dans le dispositif de culture pour conserver la MEC sur la surface du PMP fonctionnalisé pendant le traitement des cellules. Les spots de MEC sur les chips analysables directement au SELDI-TOF fournissent une nouvelle gamme de surfaces chromatographiques qui permettraient de découvrir de nouveaux biomarqueurs. D'autres applications peuvent être envisagées comme l'analyse de la différentiation cellulaire, ou l'étude de l'activité protéolytique de fluides biologiques sur la MEC
The aim of our project is the conception of extracellular matrix (ECM) surfaces on arrays suitable for direct analysis by SELDI-TOF mass spectrometry. The ECM is produced by cells in culture on the arrays, then, specific treatments remove the cells. On commercially available arrays made of aluminium, only silica spots allow the culture of human smooth muscle cells (hSMC) and endothelial cells (HUVEC) and the obtention of their ECM. According to their analysis with SELDI-TOF, the ECM of hSMC and HUVEC are different Nevertheless, opacity of aluminium arrays makes the cell observation difficult We are the first showing the us< of transparent arrays made of polymethylpentene (PMP) for SELDI-TOF analysis. As cells can not be cultured directly on PMP, we functionalized PMP arrays by gas plasma treatment to allow cell culture. Improvements are now required on the cell culture System to prevent the removal of the ECM from the arrays during the cell detachment treatment. The surfaces of ECM on arrays can be either analyzed directly by mass spectrometry or be used as a biomimetic retention surface in order to discover new biomarkers contained in biological fluids ECM-arrays could also be useful to study cell differentiation and protease activities on ECM surface

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Xu, Zeyuan. "PTMomics : Microfluidics for post-translational modifications studies : application to glycoproteomics." Electronic Thesis or Diss., Université Paris sciences et lettres, 2023. http://www.theses.fr/2023UPSLS029.

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Les glycoprotéines jouent un rôle fondamental dans la vie. Des études récentes montrent qu'elle est largement présente dans les activités biologiques, telles que la liaison des récepteurs, la signalisation cellulaire, la réponse immunitaire, le repliement des protéines et l'action des hormones. Parallèlement, la glycosylation des protéines a été associée au pronostic et à l'évolution de maladies comme les cancers. Ainsi, elle devient un biomarqueur important, et est également significative pour le développement thérapeutique. La glycosylation mérite donc d'être étudiée en détail. La glycoprotéine acquiert sa fraction d'hydrate de carbone par un processus appelé modification post-traductionnelle (PTM). Ce qui est différent de la plupart des PTM, c'est qu'il existe plusieurs formes de glycosylation. Les glycosylations N- et O-liées sont parmi les types les plus courants. D'autres comme la C-mannosylation et la phosphosérine sont rares. Si l'on considère simplement la glycosylation liée à l'azote, on peut la diviser en types complexes, hybrides et à haute teneur en mannose, qui résultent d'une synthèse non dirigée par un modèle. Sur la membrane du RE, un oligosaccharide sur Dol-PP est transféré à Asn dans la séquence Asn-X-Thr/Ser (X n'est pas la proline). Ensuite, les glycosidases et les glycosyltransférases agissent pour éliminer le glucose et ajouter une fraction de sucre dans le RE et le Golgi. Leurs activités dépendent des espèces, des types de cellules, des protéines, des sites et de l'état physiologique de la cellule, ce qui donne lieu à divers glycanes N-liés. Et l'hétérogénéité sera un obstacle majeur à l'étude des glycoprotéines. Les informations sur l'occupation de plusieurs sites peuvent être compliquées par le faible rendement d'ionisation et la fragmentation (macro-hétérogène). Sur chaque site, de multiples glycoformes existent, ce qui entraîne une diminution du signal (micro-hétérogène). De nombreux efforts ont été investis pour résoudre ce problème. La spectrométrie de masse (MS) est maintenant progressivement appliquée à l'étude de la glycosylation des protéines pour sa sensibilité, sa vitesse et son haut débit. Le couplage avec un système de chromatographie liquide à haute performance (HPLC) est une combinaison largement adoptée. Dans le même temps, des glycosidases, par exemple l'endo H et la PNGase F, sont découvertes et utilisées pour la libération des glycanes. La microfluidique est apparue pour le traitement des échantillons, et les approches hybrides sont de plus en plus populaires et devraient être la solution. En conclusion, pour une étude approfondie de sa fonction, l'occupation du site, la composition, la quantité et d'autres informations sont essentielles. Un protocole d'analyse amélioré doit être mis en œuvre pour acquérir spécifiquement ces informations à haute sensibilité, ce qui constitue le point d'ancrage de l'étude glycoprotéomique
Glycoprotein acts as a fundamental undertaker of life. Recent studies show that it is widely found in biological activities, such as in receptor binding, cell signaling, immune response, protein folding, and hormone action. Meanwhile, protein glycosylation has been associated to prognosis and progress of diseases like cancers. Thus, it becomes an important biomarker, and as well as is significant to therapeutics development. Thus, glycosylation worth to be studied in detail. Glycoprotein gains its carbon hydrate moiety through a process so called post translational modification (PTM). What is different from most of the PTMs is that there are various forms of glycosylation. N- and O-linked glycosylation are among the most common types. Others like C-mannosylation and phosphoserine are rare. Merely looking at the N-linked glycosylation, it can be further divided into complex, hybrid, and high mannose types, which is a result of the non-template-driven synthesis. On the ER membrane, an oligosaccharide on Dol-PP is transferred to Asn in the sequence Asn-X-Thr/Ser (X is not proline). Then, glycosidases and glycosyltransferases act in to remove glucose and adding sugar moiety in ER and Golgi. Their activities depend on the species, cell types, protein, sites, and physiological state of the cell resulting in various N-linked glycans. And heterogeneity will be a major hurdle to study glycoprotein. Multiple sites occupancies information can be complicated by the poor ionization yield and fragmentation (macro-heterogenous). On each site, multiple glycoforms existed leading to even diminished signal(micro-heterogenous). Many efforts have been invested in to solve it. Mass spectrometry (MS) is now progressively applied to protein glycosylation study for its sensitivity, speed, and high throughput. The coupling with a high-performance liquid chromatography system (HPLC) is a widely adopted combination. In the meantime, glycosidase, for example, endo H and PNGase F are discovered and used for glycan release. Microfluidics emerged for sample treatment, and hybrid approaches are becoming increasingly popular and expected to be the solution. To conclude, for an in-depth study of its function, site occupancy, composition, quantity, and other information are essential. An improved analysis protocol should implemented to specifically acquire this information at high sensitivity, which is the anchor for glycoproteomic study

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Guerandel, Cyril. "Etude de la qualité du piégeage des matières organiques par la matrice cimentaire vis-à-vis de la lixiviation." Thesis, Metz, 2009. http://www.theses.fr/2009METZ030S/document.

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Dans le cadre de la qualité environnementale des matériaux, il est essentiel d'apporter la preuve que les matériaux à base de ciments adjuvantés ne relarguent pas ou peu de matières organiques lors de leur contact avec l'eau constituant une solution lixiviante. Les additifs organiques, tels que les agents de mouture et les superplastifiants, constituent deux classes d'adjuvants organiques utilisés de manière systématique dans la fabrication ou la formulation des matériaux cimentaires, notamment quand ils sont en contact avec l'eau potable (conduites et châteaux d'eau). Pour évaluer le piégeage de ces composés organiques par une pâte de ciment CEM I, cinq montages de lixiviation dynamique CTG-LEACHCRETE ont été mis en place et adaptés pour l'étude de pâtes de ciment formulées avec des adjuvants organiques. La seconde partie de ce travail a pour objectif de mettre au point des techniques analytiques sensibles pour la détection de traces des constituants du superplastifiant et de l'agent de mouture directement dans les produits de la lixiviation de pâtes de ciment (les lixiviats) grâce aux techniques de spectrométrie de masse MALDI-TOF et Py-THM-MS. Enfin, l'application du protocole global de "lixiviation dynamique couplée à la spectrométrie de masse" nous permet d'apprécier la présence des composés organiques suite à des essais de lixiviation de pâtes de ciment formulées avec de l'agent de mouture et du superplastifiant. Cette démarche nous a permis d'obtenir de nombreux résultats donnant des informations sur les mécanismes de piégeage des différents additifs organiques par une pâte de ciment
Evidence that materials used by the industry are not damageable for the environment has become a major issue. In cement industry, organic admixtures such as grinding aids or superplasticizers ar widely used. In particular, they constitute cementitious materials in concrete contacting water like in water pipes and water tower. It is therefore essential to test whether these organic coumpounds are enventually dissolved into water by leaching. In this aim, five different dynamic leaching tests were developed and applied to a CEM I cement paste formulated with organic admixtures. In paralell, highly sensitive analytical methods based on MALDI-TOF and Py-THM-MS mass spectrometry techniques were designed in order to detect traces of leached superplasticizers or grinding aids. The dynamic leaching tests coupled to mass spectrometry allowed us to detect the presence of organic compounds in the leachate, and to better understand the mechanisms involved in the trapping of additives into a cement paste

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Alves, Sandra. "Une échelle acido-basique pour une meilleure compréhension du rôle de la matrice dans la production d'ions multichargés : une approche thermo-cínétique de l'ionisation en mode MALDI." Paris 6, 2002. http://www.theses.fr/2002PA066005.

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Mallah, Khalil. "In depth systemic biology analysis of central nervous system injuries." Thesis, Lille 1, 2018. http://www.theses.fr/2018LIL1S108/document.

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Dans un contexte d’étude des altérations biologiques survenant après un impact sur le système nerveux central (SNC), ma thèse porte sur l’étude des modifications protéomiques et lipidiques survenant après une lésion du SNC. Une étude spatio-temporelle a été menée sur un modèle TBI de rat afin d'identifier des marqueurs spécifiques de la lésion. En utilisant le MALDI-MSI, nous avons effectués une reconstruction 3D du cerveau lésé 3 jours post-lésion et nous avons représentés les molécules lipidiques spécifiques à la lésion. Après, cette analyse est réalisé avec d’autres délais après l’impact: 1, 3, 7 et 10 jours. En parallèle, une analyse microprotéomique est réalisée sur des coupes de tissus dans une approche visant à corréler les modifications lipidiques et protéiques. Nos résultats ont permis d'identifier une famille de lipides, les acylcarnitines, exprimés dans le cortex lésé avec une intensité maximale à 3 jours post-impact. Les données de protéomiques ont montrés une régulation positive de l’expression de protéines liées à la maladie de Parkinson. Dans l’ensemble, nos résultats décrivent un lien entre le TBI léger et la maladie de Parkinson dès 3 jours après l’impact, avec un rôle possible de l’acylcarnitine. Cette même famille de molécules est aussi présente dans les lésions médullaires. Dans une approche thérapeutique, les résultats précédents ont montrés que la protéine RhoA est un candidat majeur dans SCI. Après avoir utilisé un inhibiteur de RhoA, une étude protéomique a été réalisée pour évaluer l’impact sur ces lésions. Les résultats montrent que les traitements in-vivo et in-vitro avec l’inhibiteur stimule la croissance neuritique et la régénération axonale
In the context of studying biological alterations occurring post impact to the central nervous system, my thesis was focused on studying the proteomic and lipid changes occurring post injury to the brain and spinal cord. A fundamental spatio-temporal study was conducted on an open-head rat TBI model to identify potential injury-specific markers. Using MALDI MSI, we performed 3D reconstruction of the injured brain at 3 days after injury and depicted lesion-specific m/z lipid molecules. After, MALDI MSI was applied on the acute/sub-acute time frame post impact: 1 day, 3 days, 7 days, and 10 days. In parallel, a microproteomic analysis was carried out on tissue segments directly consecutive to the imaged ones in an approach to correlate both lipid and protein changes. Our results yielded the identification of a family of lipids, acylcarnitines, which are expressed within the injured cortex with maximum intensity 3 days post impact. These lipid molecules also were found to be expressed in the substantia nigra and microproteomics data showed an upregulation in expression of Parkinson’s related proteins. Taken altogether, our results depict a role of link between mild-TBI and Parkinson’s disease as early as 3 days post impact, with a possible role of acylcarnitine. This same family of molecules was also present in SCI. In a therapeutic approach previous results showed RhoA protein as a major candidate post impact in SCI. After using RhoA inhibitor treatment, a proteomic study was carried out to investigate its impact on SCI. The results showed that both in-vivo and in-vitro treatment with RhoA inhibitor stimulated neurite outgrowth and helped in axonal regeneration

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Crank, Jeffrey Aaron. "Ionic liquids MALDI-MS matrices and gas chromatography stationary phases /." [Ames, Iowa : Iowa State University], 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3379180.

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Hellwig, Nils [Verfasser]. "Das Laserablationsverhalten von ionischen Flüssigkeiten verschiedener MALDI-Matrices / Nils Hellwig." Kiel : Universitätsbibliothek Kiel, 2012. http://d-nb.info/1025465121/34.

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Allwood, Daniel Anthony. "Characterisation and ionisation modelling of matrices in MALDI mass spectrometry." Thesis, University of Hull, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301477.

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Книги з теми "Matrice MALDI"

Liang, Li, ed. MALDI mass spectrometry for synthetic polymers analysis. Hoboken: Wiley, 2010.

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Tambe, Suparna. Structure Performance Relationships of the Novel Maldi-MS Matrices. Logos Verlag Berlin, 2018.

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Hillenkamp, Franz, and Jasna Peter-Katalinic. Maldi MS: A Practical Guide to Instrumentation, Methods and Applications. Wiley & Sons, Incorporated, John, 2013.

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Hillenkamp, Franz, and Jasna Peter-Katalinic. Maldi MS: A Practical Guide to Instrumentation, Methods and Applications. Wiley & Sons, Limited, John, 2007.

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Hillenkamp, Franz, and Jasna Peter-Katalinic. Maldi MS: A Practical Guide to Instrumentation, Methods and Applications. Wiley & Sons, Incorporated, John, 2013.

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Hillenkamp, Franz, and Jasna Peter-Katalinic. Maldi MS: A Practical Guide to Instrumentation, Methods and Applications. Wiley & Sons, Limited, John, 2013.

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Hillenkamp, Franz, and Jasna Peter-Katalinic. Maldi MS: A Practical Guide to Instrumentation, Methods and Applications. Wiley & Sons, Incorporated, John, 2013.

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Maldi MS: A Practical Guide to Instrumentation, Methods, and Applications. Wiley-VCH Verlag GmbH, 2013.

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Hillenkamp, Franz, and Jasna Peter-Katalinic. Maldi MS: A Practical Guide to Instrumentation, Methods and Applications. Wiley & Sons, Incorporated, John, 2007.

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Riley, Peter. The role of the microbiology laboratory in antimicrobial stewardship. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780198758792.003.0010.

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Microbiology laboratories play an important role in antimicrobial stewardship at the level of individual patients and the population as a whole. When empiric therapy has been started, rapid results can lead to earlier targeted treatment. Accumulated results of susceptibility tests can be analysed and used to generate local or national guidelines on empiric treatment and prophylaxis. Several methods can be used to determine microbial identity and antimicrobial susceptibility, including traditional culture-based methods and newer molecular methods such as matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, polymerase chain reaction (PCR), and whole-genome sequencing. These methods and potential advantages are reviewed. Before results are reported, expert rules are applied and results edited. At this point the laboratory can influence prescribing practices.

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Частини книг з теми "Matrice MALDI"

Hossain, Mahmud, and Patrick A. Limbach. "A Comparison of MALDI Matrices." In Electrospray and MALDI Mass Spectrometry, 215–44. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2012. http://dx.doi.org/10.1002/9780470588901.ch7.

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Santos, Cledir, Paula Galeano, Reginaldo Lima Neto, Manoel Marques Evangelista Oliveira, and Nelson Lima. "MALDI-TOF MS and its requirements for fungal identification." In Trends in the systematics of bacteria and fungi, 119–40. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789244984.0119.

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Abstract Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is now used as a routine technique for the fast and reliable identification of fungi at the species level and, currently, it represents an important phenotypic methodology based on proteomic profiles. The main limitations to MALDI-TOF MS for fungal identification are related to sample quality (e.g. quality of biological material such as rigidity or pigmentation of cell walls), sample preparation (e.g. the myriad of sample preparation methodologies that deliver different data sets to different MALDI-TOF MS databases) and the databases themselves (e.g. the 'black-box' commercial databases). This chapter presents an overview and discussion of the use of MALDI-TOF MS for fungal identification. The major known limitations of the technique for fungal taxonomy, and how to overcome these, are also discussed.

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O'Connor, Peter B. "The Development of Matrix-Assisted Laser Desorption/Ionization Sources." In Electrospray and MALDI Mass Spectrometry, 185–213. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2012. http://dx.doi.org/10.1002/9780470588901.ch6.

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Bahr, U., and T. W. Jaskolla. "Employing ‘Second Generation’ Matrices." In Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry, 3–35. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-04819-2_1.

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Tucher, Joanna, Prasath Somasundaram, and Andreas Tholey. "Quantitative MALDI MS Using Ionic Liquid Matrices." In Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry, 77–90. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-04819-2_5.

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Mathur, Sonal, Alexis Nazabal, and Renato Zenobi. "Probing Noncovalent Interactions by Electrospray Ionization and Matrix-Assisted Laser Desorption/Ionization." In Electrospray and MALDI Mass Spectrometry, 535–70. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2012. http://dx.doi.org/10.1002/9780470588901.ch15.

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Towers, Mark W., and Rainer Cramer. "Ionic Liquids and Other Liquid Matrices for Sensitive MALDI MS Analysis." In Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry, 51–64. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-04819-2_3.

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Karas, M., and U. Bahr. "Matrix-Assisted Laser Desorption-Ionization (MALDI) Mass Spectrometry of Biological Molecules." In Mass Spectrometry in Biomolecular Sciences, 33–49. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-009-0217-6_2.

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Karas, M., and U. Bahr. "Matrix-Assisted Laser Desorption-Ionization (MALDI) Mass Spectrometry: Principles and Applications." In Selected Topics in Mass Spectrometry in the Biomolecular Sciences, 33–53. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5165-8_3.

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Dilmetz, Brooke A., Peter Hoffmann, and Mark R. Condina. "Quantitative Approach Using Matrix-Assisted Laser Desorption/ Time-of-Flight (MALDI-ToF) Mass." In Methods in Molecular Biology, 159–66. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1024-4_12.

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Тези доповідей конференцій з теми "Matrice MALDI"

Murray, Kermit K., Michelle D. Beeson, and David H. Russell. "Laser Ionization of Biomolecules in Solution." In Laser Applications to Chemical Analysis. Washington, D.C.: Optica Publishing Group, 1994. http://dx.doi.org/10.1364/laca.1994.tha.5.

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Many powerful laser based methods are unavailable for the analysis of molecules in solution. Techniques for the analysis of liquids are particularly important for the study of biomolecules, whose natural environment is a water solution. Mass spectrometry is a powerful analytical technique, but liquids and mass spectrometers are fundamentally incompatible. We have developed a technique for laser ionization of biomolecules in solution by applying matrix-assisted laser desorption ionization (MALDI) to liquid aerosols. In the typical MALDI experiment, the analyte biomolecule is deposited from solution onto a metal surface with a 100 to 50,000 molar excess of a suitable matrix, usually a UV absorbing organic acid.1 The solvents are allowed to evaporate and the sample is inserted into the source region of a mass spectrometer. Light from a pulsed laser is absorbed by the matrix causing both ablation of the surface and ionization of the intact biomolecule. In the aerosol MALDI experiment, 2,3 the analyte biomolecule is dissolved in a methanol solution with an ultraviolet absorbing matrix. The aerosol is sprayed into vacuum, desolvated, and ionized by pulsed UV laser radiation. The ions are mass separated in a time-of-flight (TOF) mass spectrometer. Aerosol MALDI mass spectra have been obtained for a variety of peptides and proteins with molecular weights as large as 80,000. We have used aerosol MALDI as a liquid chromatography detection method4 (LC/MS) and as a probe of aerosol and cluster chemistry.5 This paper gives a general description of aerosol MALDI and discusses some recent results for peptide and protein ionization.

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Kinsel, Gary R., Kent Gillig, Ricky Edmondson, and David H. Russell. "Fundamental Investigations of the Mechanism of Laser Desorption and Ionization in Matrix Assisted Laser Desorption / Ionization." In Laser Applications to Chemical Analysis. Washington, D.C.: Optica Publishing Group, 1994. http://dx.doi.org/10.1364/laca.1994.thb.1.

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The recent development of Matrix Assisted Laser Desorption / Ionization (MALDI) has sparked a revolution in the field of high molecular weight mass spectrometry.1 Time-of-flight (TOF) mass spectra of proteins weighing up to 300,000 Da are now routinely produced and this achievement has fostered a variety of bioanalytical applications which were previously unapproachable using conventional mass spectrometric techniques. These successful applications have burgeoned in spite of a poor understanding of the mechanism of analyte desorption and ionization under MALDI conditions. An improved understanding of the MALDI mechanism should aid in overcoming a number of limitations of the current state-of-the-art and forms the motivation for the work described.

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Bridgmohan, Chelsea, Lichang Wang, and Kristopher Kirmess. "INSIGHTS INTO PROTON TRANSFER MECHANISMS IN HALOGEN-SUBSTITUTED MALDI MATRICES." In 73rd International Symposium on Molecular Spectroscopy. Urbana, Illinois: University of Illinois at Urbana-Champaign, 2018. http://dx.doi.org/10.15278/isms.2018.wj10.

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Scott, C. T. J., C. Kosmidis, W. J. Jia, K. W. D. Ledingham, and R. P. Singhal. "Investigations of desorbed species from matrix materials used in MALDI." In The 7th international symposium: Resonance ionization spectroscopy 1994. AIP, 1995. http://dx.doi.org/10.1063/1.47635.

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Bridgmohan, Chelsea, Kristopher Kirmess, and Lichang Wang. "INSIGHT INTO THE CHARGE TRANSFER MECHANISMS OF HEAVY ATOM SUBSTITUTED MALDI MATRICES." In 72nd International Symposium on Molecular Spectroscopy. Urbana, Illinois: University of Illinois at Urbana-Champaign, 2017. http://dx.doi.org/10.15278/isms.2017.wi05.

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Kobylis, Paulina, Hanna Lis, Piotr Stepnowski, and Magda Caban. "Verification of the homogeneity of the matrix/analyte mixture on sample plate using MALDI-MS technique and new ionic liquid matrices." In Człowiek Nauka Środowisko. Institute of Biotechnology and Molecular Medicine Foundation, 2018. http://dx.doi.org/10.31708/spi3.18/pkoby.cns18.

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Drake, Wonder, Erin Seeley, and Richard Caprioli. "Matrix-Assisted Laser Desorption/Ionization (MALDI) Imaging Mass Spectrometry (IMS) Localize Mycobacterial Proteins To Sarcoidosis Granulomas." In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a3984.

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Hafenstine, Glenn. "Matrix-assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry for Advanced Polymer Charaterization." In 46th Polymeric Materials, Adhesives, and Composites (PolyMAC) Conference; KCNSC; June 21-23, 2022; KCNSC. US DOE, 2022. http://dx.doi.org/10.2172/1872439.

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Shah, Neeraj, Rebecca Gorton, Jennifer Canizales, Gina Birch, Timothy McHugh, and Marc Lipman. "The value of rapid speciation of nontuberculous mycobacteria (NTM) by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF)." In Annual Congress 2015. European Respiratory Society, 2015. http://dx.doi.org/10.1183/13993003.congress-2015.pa370.

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Lee, Adam Michael, Jessica Moertel, Kangsheng Wang, and Robert B. Diasio. "Abstract 5451: High-throughput detection of dihydropyrimidine dehydrogenase gene (DPYD) variants using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF)." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-5451.

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Звіти організацій з теми "Matrice MALDI"

Puretzky, A. A., and D. B. Geohegan. LIF-imaging and gas-phase diagnostics of laser desorbed MALDI-matrix plumes. Office of Scientific and Technical Information (OSTI), July 1997. http://dx.doi.org/10.2172/563317.

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Thurlow, James. 2018 Social Accounting Matrix for Mali: A Nexus Project SAM. Washington, DC: International Food Policy Research Institute, 2021. http://dx.doi.org/10.2499/p15738coll2.134818.

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Korte, Andrew R. Development of matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) for plant metabolite analysis. Office of Scientific and Technical Information (OSTI), December 2014. http://dx.doi.org/10.2172/1226566.

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Fluhr, Robert, and Maor Bar-Peled. Novel Lectin Controls Wound-responses in Arabidopsis. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7697123.bard.

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Innate immune responses in animals and plants involve receptors that recognize microbe-associated molecules. In plants, one set of this defense system is characterized by large families of TIR–nucleotide binding site–leucine-rich repeat (TIR-NBS-LRR) resistance genes. The direct interaction between plant proteins harboring the TIR domain with proteins that transmit and facilitate a signaling pathway has yet to be shown. The Arabidopsis genome encodes TIR-domain containing genes that lack NBS and LRR whose functions are unknown. Here we investigated the functional role of such protein, TLW1 (TIR LECTIN WOUNDRESPONSIVE1). The TLW1 gene encodes a protein with two domains: a TIR domain linked to a lectin-containing domain. Our specific aim in this proposal was to examine the ramifications of the TL1-glycan interaction by; A) The functional characterization of TL1 activity in the context of plant wound response and B) Examine the hypothesis that wounding induced specific polysaccharides and examine them as candidates for TL-1 interactive glycan compounds. The Weizmann group showed TLW1 transcripts are rapidly induced by wounding in a JA-independent pathway and T-DNA-tagged tlw1 mutants that lack TLW1 transcripts, fail to initiate the full systemic wound response. Transcriptome methodology analysis was set up and transcriptome analyses indicates a two-fold reduced level of JA-responsive but not JA-independent transcripts. The TIR domain of TLW1 was found to interact directly with the KAT2/PED1 gene product responsible for the final b-oxidation steps in peroxisomal-basedJA biosynthesis. To identify potential binding target(s) of TL1 in plant wound response, the CCRC group first expressed recombinant TL1 in bacterial cells and optimized conditions for the protein expression. TL1 was most highly expressed in ArcticExpress cell line. Different types of extraction buffers and extraction methods were used to prepare plant extracts for TL1 binding assay. Optimized condition for glycan labeling was determined, and 2-aminobenzamide was used to label plant extracts. Sensitivity of MALDI and LC-MS using standard glycans. THAP (2,4,6- Trihydroxyacetophenone) showed minimal background peaks at positive mode of MALDI, however, it was insensitive with a minimum detection level of 100 ng. Using LC-MS, sensitivity was highly increased enough to detect 30 pmol concentration. However, patterns of total glycans displayed no significant difference between different extraction conditions when samples were separated with Dionex ICS-2000 ion chromatography system. Transgenic plants over-expressing lectin domains were generated to obtain active lectin domain in plant cells. Insertion of the overexpression construct into the plant genome was confirmed by antibiotic selection and genomic DNA PCR. However, RT-PCR analysis was not able to detect increased level of the transcripts. Binding ability of azelaic acid to recombinant TL1. Azelaic acid was detected in GST-TL1 elution fraction, however, DHB matrix has the same mass in background signals, which needs to be further tested on other matrices. The major findings showed the importance of TLW1 in regulating wound response. The findings demonstrate completely novel and unexpected TIR domain interactions and reveal a control nexus and mechanism that contributes to the propagation of wound responses in Arabidopsis. The implications are to our understanding of the function of TIR domains and to the notion that early molecular events occur systemically within minutes of a plant sustaining a wound. A WEB site (http://genome.weizmann.ac.il/hormonometer/) was set up that enables scientists to interact with a collated plant hormone database.

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