Добірка наукової літератури з теми "Mappia foetida"

In this paper the novel green synthesis of cobalt oxide nanoparticles (Co3O4NPs) from cobalt chloride (CoCl2) using Mappia foetida leaf extract was investigated. The characterization of the Co3O4NPs was done by using UV – Vis spectroscopy, EDX, XRD and SEM analysis techniques. Comparative antibacterial study was done against gram positive and gram negative bacteria by well diffusion method in which results revealed that the biologically synthesized Co3O4NPs showed relatively similar antibacterial potential as chemically synthesized Co3O4NPs and higher antibacterial potential than that of positive control.

Cancer has become the leading disease-related cause of death in the human population. For example, in the United States, cancer is the second leading cause of death behind cardiovascular disease, and it is projected that cancer will become the leading cause of death in the coming years. The medical treatment of cancer still has many unmet needs. The main curative therapies for cancer, surgery and radiation, are generally only successful if the cancer is found at an early localized stage. Once cancer has progressed to metastatic stage, these therapies are less successful. Hence, chemotherapeutic drugs are used for the treatment of these advanced tumors, particularly in the case of the common epithelial tumors such as lung, colorectal, breast, prostate, and pancreatic cancers. New chemotherapeutic drugs are necessary since most of the cancers acquire resistance towards existing anticancer drugs. Nature has always been an attractive source of new chemotherapeutic agents, as a tremendous chemical diversity is found in millions of species of plants, animals, and microorganisms.Microorganisms (bacteria, fungi, actinomycetes) serve as readily renewable and inexhaustible source of novel bioactive metabolites.Endophyte, a microorganism that reside in theinternal tissues of living plantswithout causing any immediate overt negative effects, are potential sources of novel natural products for exploitation in medicine. Mappia foetidais distributed in western part of peninsular coastal India from Konkanghatsto northern parts of the Kanara, Nilgiris, Anamalis, and Pullneys hills of India. It is a rich source of alkaloids such as camptothecin, 9-methoxy camptothecin, mappicin,sitosterol and lupeol and other natural products having anticancer and antimicrobial properties. Since, the secondary metabolite production in fungal endophytes is greatly influenced by theircompetitive ecological niche and interaction with host metabolism, Mappia foetida was chosen for endophytic fungal isolation. Sirsi, (North Karnataka, India) because of its rich biodiversity was chosen as a location for collection of the plant samples. The organicsolvent extracts obtained from mycelia and culture filtrates of all the endophytic fungal isolates were screened for their cytotoxic activity against HeLa (human cervical carcinoma) cellline. Organic extracts of 8 fungal isolates exhibited significant cytotoxic activity, among which Phoma tropica(S1/3) culture filtrate extract exhibited most significant cytotoxicactivity againstHeLa cell line with IC50 of 25µg/ml. Dichloromethane extract of the Phoma tropica culture filtrate was subjected to bioassay–guided column chromatographic fractionationwhich resulted in the isolation of purified cytotoxic secondary metabolite. Based on the analysis of variousspectroscopic techniques such as NMR, FTIR, LC-MS/MS, HRMS, CHNOS elemental analysis and X-ray diffraction studies, the purified metabolite was identified as 2-Hydroxy-2,4-dimethyl-5-[-1-propen-1-yl]-3(2H)furanone(Phomafuranone).Phomafuranone exhibited significant cytotoxic activity against various cancer cell lines (HeLa, Jurkat, COLO 205, HT-29, HCT-15, HCT 116, A549, A-431 and OVCAR-3) Further studies were undertaken to elucidate the mechanism of cytotoxicityof the purified metabolite on human cancer cell lines. Phomafuranone contains a conjugated unsaturated α, β, γ, δ carbonyl pharmacophore moiety. Polyunsaturated carbonyl compounds are referred to as “Michael acceptors” and they behave as soft electrophiles. Michael acceptors react with strong biological nucleophiles such as thiols.The reactivity of electrophilic Phomafuranone with thiol containing biomolecules like glutathione, cysteine and N-acetyl cysteine was investigated by spectrophotometric methods. Cell cycle progressionanalyses oftreated HCT 116 (colorectal carcinoma) cell line by flow cytometry revealed that Phomafuranone arrested significant proportion of cells in G2/M phase. HCT 116 cells were arrested specifically in early mitotic phase of cell cycle as indicated by time dependent increase in phospo-histone3 (Ser10) levels and nuclear import of Cyclin B1.On treatment cancer cells with Phomafuranone, time dependent depletion of intracellular reduced glutathione levels was observed. Depletion of reduced glutathione inturn led to elevated intracellular ROS levels.Pre-treatment of HCT 116 cell lines with thiol containing antioxidants like N- acetylcysteine (NAC) and reduced glutathione (GSH) completely abrogated itscytotoxic effect, suggesting Phomafuranone inducedthiol–mediated cytotoxicity. The elevated ROS levels led to mitochondrial membrane depolarization as indicated by cytochrome c release in cytosol from mitochondria in a time dependent manner. Cytochrome c release was followed caspase 9 mediated apoptotic cell death. Thus, our results suggest that Phomafuranone induced redox imbalancemediated apoptosis in HCT 116 cell line by intrinsic pathway.

Introdução: Os resíduos orgânicos quando descartados de maneira inadequada auxiliam na proliferação de vetores, que são agentes transmissores de doenças, e na formação de um composto líquido denominado chorume, oferecendo riscos ambientais e na saúde pública. A agricultura é responsável por gerar alimentos, a produção que segue para as cidades é para guarnecer supermercados, feiras, centros de distribuição, durante todas as etapas ocorre a geração de resíduos orgânicos. Uma forma correta de tratar esses resíduos é a vermicompostagem, um processo biotecnológico de eficácia e ambientalmente correta. Tendo como principal atuante a minhoca da espécie Eisenia foetida que possuem a função de acelerar o processo de decomposição natural do resíduo orgânico. Objetivo: O objetivo do trabalho foi tratar os resíduos orgânicos gerados no setor de hortifrutigranjeiros pelo processo de vermicompostagem. Material e métodos: O experimento foi realizado no laboratório de Ecotoxicologia e Resíduos de uma Instituição de Ensino Superior. Os resíduos orgânicos utilizados foram cedidos por um comércio de distribuição alimentícia localizado na cidade de Pelotas, RS. Para as vermicomposteiras utilizou-se duas caixas plásticas de 20 L e foram adicionados resíduos orgânicos, adubo e minhocas da espécie eusenia foetida em cada vermicomposteira, após a montagem do experimento foi feito o monitoramento da temperatura ambiente e coletas mensais para análises laboratoriais, assim como no fim do processo ocorreu a contagem das minhocas que foram classificadas em jovens e adultas. Resultados: Os valores da temperatura, pH, condutividade elétrica e umidade para o vermicomposto ficaram dentro do limite recomendado pela instrução normativa 25/2009 - SDA/MAPA em seu Anexo III, dispõe limites de tolerância, a partir de parâmetros, na produção de vermicomposto, assim como a reprodução das minhocas que obteve resultados satisfatórios. Conclusão: Constata-se que a vermicompostagem é uma alternativa viável para o tratamento dos resíduos, gerando um produto de valor agronômico e ambiental.