Дисертації з теми "Mammalian models"
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Weis, Michael Christian. "Computational Models of the Mammalian Cell Cycle." Case Western Reserve University School of Graduate Studies / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1323278159.
Повний текст джерелаRabani, Michal. "Models of dynamic RNA regulation in mammalian cells." Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/84894.
Повний текст джерелаCataloged from PDF version of thesis.
Includes bibliographical references (pages 135-142).
Complex molecular circuits, consisting of multiple intertwined feedback loops and non-linear interactions, are a hallmark of every living cell, and a model of a dynamic complex network. Here, I systematically study the dynamic changes in the cellular circuits that control RNA levels in mammalian cells, focusing on the model response of immune dendritic cells to pathogens, through an integration of comprehensive computational models and innovative empirical approaches. I establish a computational framework to follow the dynamics of processes for RNA birth (production, by transcription), maturation (processing), and death (degradation), and their integration in the dynamic RNA life cycle. I study the kinetics of a gene's RNA population with a model of its production and degradation, and generalize the system as an ensemble of genes. I further model genes as composite particles and study the regulation and kinetics of altering their internal structure. To allow robust statistical inference from these models, I develop innovative laboratory assays and collect extensive experimental data on the system. I directly measure RNA production rates by coupling short RNA metabolic labeling with advanced RNA quantification. I leverage recent improvements in RNA quantification by next-generation sequencing technology, to significantly increase the resolution of metabolic labeling in both time and gene-structure. Finally, I collect perturbation data, by monitoring RNA levels when specific elements of the network are disabled. In this way, I formulated several general principles of RNA regulation and its temporal evolution in mammalian cells. I find that temporal changes in production provide a dominant input in computing RNA levels by the cell over time. Yet, dynamic degradation changes contribute to shaping expression peaks, and dynamic processing changes allow a fast accumulation of mature transcripts. Static degradation and processing rates vary between genes and between individual splicing junctions, consistently with their function and expression dynamics. This study is broadly applicable to many normal as well as diseases misregulated cellular networks, and is also relevant for a more general analysis of complex systems dynamics.
by Michal Rabani.
Ph.D.
Shaw, Alexander George. "Developing models of the mammalian cell S phase." Thesis, University of Manchester, 2011. https://www.research.manchester.ac.uk/portal/en/theses/developing-models-of-the-mammalian-cell-s-phase(3df7caaf-fd64-4bd2-b500-802f1a2c8ce2).html.
Повний текст джерелаPatel, Nirmal Praful School of Medicine UNSW. "Olfactory progenitor cell transplantation into the mammalian inner ear." Awarded by:University of New South Wales. School of Medicine, 2006. http://handle.unsw.edu.au/1959.4/26180.
Повний текст джерелаNel, Maria Elizabeth. "Mammalian cell cultures as models for metabolomic studies / Nel Z." Thesis, North-West University, 2012. http://hdl.handle.net/10394/8205.
Повний текст джерелаThesis (MSc (Biochemistry))--North-West University, Potchefstroom Campus, 2012.
Hayes, Chris. "Genetic and functional analysis of mammalian limb development." Thesis, University of Oxford, 1998. http://ora.ox.ac.uk/objects/uuid:1443b218-fb63-4ced-9b15-e81947448ced.
Повний текст джерелаSiepel, Adam C. "Comparative mammalian genomics : models of evolution and detection of functional elements /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2005. http://uclibs.org/PID/11984.
Повний текст джерелаDalle, Pezze Piero. "Dynamical models of the mammalian target of rapamycin network in ageing." Thesis, University of Newcastle upon Tyne, 2013. http://hdl.handle.net/10443/2183.
Повний текст джерелаRoopra, Avtar. "Transcriptional control of the m4 muscarinic receptor gene : mammalian and yeast models." Thesis, University College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313823.
Повний текст джерелаZaksauskaite, Ringaile. "Analysis of chromosomal protein-linked break repair in zebrafish and mammalian models." Thesis, University of Sheffield, 2018. http://etheses.whiterose.ac.uk/20765/.
Повний текст джерелаCrosatti, Marialuisa. "Use of non-mammalian models to assess the virulence of Pseudomonas aeruginosa." Thesis, University of Leicester, 2015. http://hdl.handle.net/2381/32941.
Повний текст джерелаGuilliatt, Andrea Marie. "Expression of Von Willebrand Factor in mammalian cell lines as models of Von Willebrand's Disease." Thesis, University of Sheffield, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284766.
Повний текст джерелаHwang, Dick G. "Bayesian Markov chain Monte Carlo phylogenetic analysis of mammalian evolution reveals varying substitution patterns along the sequence and across lineages /." Thesis, Connect to this title online; UW restricted, 2005. http://hdl.handle.net/1773/10294.
Повний текст джерелаLupi, Daniela. "The entrainment pathway of the mammalian circadian system : a study of retinally degenerative and normal rodent models." Thesis, Imperial College London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368088.
Повний текст джерелаThompson, Airlia Camille Simone. "Mediators and markers of mammalian lifespan extension| Proteomic, proliferative and hormonal adaptations in mouse models of extended lifespan." Thesis, University of California, Berkeley, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=3640662.
Повний текст джерелаAging is broadly defined as the deterioration of bodily tissues over time. Excluding death due to infectious disease or accidents, aging is what ultimately places finite limits on lifespan and healthspan, the time in which in individual remains active and in good health. Although healthspan and lifespan are intimately linked, it is the extension of human healthspan that is a major goal of gerontological research. Such an achievement would have broad social and economic benefits and importantly would mitigate the dire consequences of the predicted future rise in the prevalence of age-related diseases due to the growing proportion of the population that is of advanced age (65yr and over). A broad understanding of the physiological, metabolic, hormonal, cellular and molecular factors that contribute to aging and lifespan determination will allow for the development of strategies to extend healthspan.
The work presented herein describes the use of three unique mouse models of extended healthspan and maximum lifespan, including calorie restricted (CR), Snell Dwarf and rapamycin-treated mice, to investigate several factors linked to healthspan and lifespan determination in mammals. These factors include reduced insulin-like growth factor-1 (IGF-1) expression, reduced cell proliferation, reduced protein synthesis and enhanced proteome stability. Using these three models the following conclusions have been drawn: 1) Physiological adaptations to CR previously suggested to confer the healthspan and lifespan benefits of CR in rodents cannot account for the global cell proliferation rate-lowering effect of CR; 2) Fibroblast growth factor 21 (FGF21) is not necessary for the reduction in IGF-1 or the reduction in global cell proliferation rates in response to moderate CR in adult mice; 3) A reduction in global cell proliferation rates is not a consistent predictor of maximum lifespan extension in mice; 4) A reduction in hepatic protein replacement rates is a sensitive and early predictor of maximum lifespan extension in mice and likely reflects a more stable and functional proteome.
Abdallah, Hussein(Hussein M. ). "The core mammalian pluripotency network in induced pluripotent stem cell (iPSC) formation : models for genetic and epigenetic reprogramming." Thesis, Massachusetts Institute of Technology, 2018. https://hdl.handle.net/1721.1/122910.
Повний текст джерелаThesis: M. Eng., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2018
Cataloged from student-submitted PDF version of thesis. "February 2018."
Includes bibliographical references (pages 23-37).
In 2006, history was made in a seminal experiment that converted mouse fibroblasts to a pluripotent phenotype coined the 'induced pluripotent stem cell' (iPSC) state. Unhindered by ethical or immunogenic constraints, iPSCs potentially hold the keys to tremendous applications in therapeutic and regenerative medicine. Furthermore, on-demand iPSC generation has the capacity to revolutionize basic research in disease modeling and drug discovery. These promises notwithstanding, the economics of iPSC formation--which remains a slow, inefficient, expensive, and laborious process--still stand in the way of fully making use of this extraordinary technology. In this thesis, I present mathematical models aimed at understanding the theoretical reprogrammability of the core pluripotency gene regulatory network being awakened in iPSC reprogramming. Using these modeling insights, I discuss the merits of current reprogramming strategies, which can be viewed as open-loop perturbations in control theoretic terms. I then discuss an alternative paradigm of closed-loop reprogramming, which is theoretically shown to be far superior when it comes to the reprogrammability of the pluripotency network. Finally, I propose a reprogramming model that incorporates the eæect of DNA demethylation on the activation of the network, with attention given to the relationship between this epigenetic transformation and the cell proliferation barrier that somatic cells seemingly face on the road to pluripotency.
by Hussein Abdallah.
M. Eng.
M.Eng. Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science
Klöting, Nora. "Phenotypic and genetic analysis in animal models and humans with type 1 diabetes or metabolic syndrome: unraveling complex mammalian diseases." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=974087254.
Повний текст джерелаMarta, Mónica Sofia Calado. "Gene regulation and immune mechanisms in multiple sclerosis experimental models /." Stockholm : Karolinska institutet, 2007. http://diss.kib.ki.se/2007/978-91-7357-280-4/.
Повний текст джерелаHayes, Samuel Lee. "Response of mammalian models to exposure of bacteria from the genus Aeromonas evaluated using transcriptional analysis and conjectures on disease mechanisms." Cincinnati, Ohio : University of Cincinnati, 2007. http://www.ohiolink.edu/etd/view.cgi?acc%5Fnum=ucin1172346920.
Повний текст джерелаTitle from electronic thesis title page (viewed Apr. 18, 2007). Includes abstract. Keywords: microarrays, transcription changes, virulence, Aeromonas. Includes bibliographical references.
Kauppila, Johanna Heta Katariina [Verfasser], Nils-Göran [Gutachter] Larsson, Aleksandra [Gutachter] Trifunovic, Jan [Gutachter] Riemer, and Jan [Gutachter] Smeitink. "Generating mammalian mitochondrial disease models with mitochondrial DNA mutations / Johanna Heta Katariina Kauppila ; Gutachter: Nils-Göran Larsson, Aleksandra Trifunovic, Jan Riemer, Jan Smeitink." Köln : Universitäts- und Stadtbibliothek Köln, 2018. http://d-nb.info/1165772744/34.
Повний текст джерелаHAYES, SAMUEL Lee. "RESPONSE OF MAMMALIAN MODELS TO EXPOSURE OF BACTERIA FROM THE GENUS AEROMONASEVALUATED USING TRANSCRIPTIONAL ANALYSIS AND CONJECTURES ON DISEASE MECHANISMS." University of Cincinnati / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1172346920.
Повний текст джерелаKade, Ige Joseph. "Interaction of organodiselenides with sulphydryl groups at the active sites of some thiol containing proteins - in vitro and in vivo mechanistic studies in mammalian models of diabetes." Universidade Federal de Santa Maria, 2008. http://repositorio.ufsm.br/handle/1/4398.
Повний текст джерелаO presente estudo quis comparar os potenciais antioxidants in vitro de organoselênios novamente sintetizados, diseleneto dicolesterol e diseleneto de difenila e suas possíveis interações com algumas enzimas contendo tióis em diferentes tecidos de mamíferos. Além disso, o potencial de DPDS como agente antioxidante e antihiperglicêmico, e sua interação com proteínas contendo tióis em vários tecidos e órgãos de mamíferos (hepático, renal, esplênico e, mais importante, tecido cerebral) foram avaliados em modelos animais de streptozotocina induzindo diabetes em ratos. Os resultados in vitro mostram que DPDS exibiu uma maior atividade mimética da glutationa peroxidase bem como aumentada habilidade para oxidar mono e di-tióis que DPDS. Além disso, enquanto o DPDS inibiu substâncias reativas ao ácido tiobarbitpúrico (TBARS) e formação de proteínas carboniladas em tecidos cerebral e hepático, induzidas por ferro(II) ou SNP, DCDS exibiu um efeito pró-oxidante em cérebro e tecido hepático quando ferro(II) serviu como próoxidante, porém, quando TBARS foi induzido por SNP, DCDS modificou a formação de TBARS tanto em tecido cerebral como hepático. Também, as atividades da deltaaminolevulinato desidratase (ð-ALA-D) cerebral e hepática e Na+/K+-ATPase cerebral foram significativamente inibidas por DPDS e somente fracamente inibida por DCDS. Mas estudos revelam que a inibição causada por organodiselenetos (neste caso, DPDS) na atividade da Na+/K+-ATPase envolve a modificação de grupos tiólicos ligados ao sítio ATP da enzima. Similarmente, diferentes isoformas da lactato desidrogenase (LDH) foram significativamente inibidas por DPDS e DCDS in vitro. nós observamos que a inibição in vitro de diferentes isoformas da LDH por DCDS e DPDS envolve a modificação de grupos SH no sítio ligante NAD+ da enzima. a administração oral de DPDS dissolvido em óleo soya administrado a ratos albino machos com diabetes induzida por streptozotocina mostrou que houve uma redução significante nos níveis de glicose sanguínea acompanhada por uma marcada redução nas proteínas glicadas em ratos diabéticos induzidos com streptozitocina tratados com DPDS em relação aos não diabéticos. Além disso, DPDS melhorou significativamente os níveis de vitamina C e GSH (fígado, rim e baço), que foram diminuídos em ratos tratados com streptozotocina. Similarmente, tratamento com DPDS marcadamente aboliu os níveis elevados de TBARS que foram observados no grupo diabético. Finalmente, a inibição da ð-ALA-D e algumas isoformas da LDH causada pela hiperglicemia foram prevenidas por DPDS. Nós também observamos que STZ provocou uma significante diminuição no status antioxidante do cérebro e atividade da Na+/K+- ATPase, mas a atividade da acetilcolinesterase e captação e liberação de glutamato não foram alteradas. Porém, DPDS marcadamente restaurou o desequilíbrio observado no status antioxidante e bomba de sódio. Finalmente, nós concluímos que organoselenetos são remédios antioxidantes promissores no manejo de doenças causadas por estresse oxidativo. Porém, sua toxicidade envolve uma interação com tióis em proteínas e este estudo demonstrou que os grupos sulfidril em questão são críticos para a função normal de enzimas e proteínas. Estes SH são associados com tióis dos sítios de ligação do substrato (sítio ativo) de enzimas. interessantemente, doses farmacológicas de organodiselenetos (3mg/kg para o estudo de diabetes) não apresentou nenhuma toxicidade observada.
Becker-Weimann, Sabine. "Modeling feedback loops in the mammalian circadian oscillator." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2010. http://dx.doi.org/10.18452/16148.
Повний текст джерелаIn many organisms the circadian clock ticks with a period of approximately 24 hours, enabling the organisms to keep track of time without any environmental time cues. Many physiological and cellular processes underlie circadian regulation. The molecular clock is a network of intracellular feedback loops: The clock gene products directly or indirectly regulate their own transcription, which results in molecular oscillations. In this thesis, existing and new mathematical models of the circadian oscillator are used to investigate the meaning of structural features - in particular of the feedback loops - for fundamental circadian characteristics. In a simple model (Goodwin model) with one negative feedback loop, saturating kinetics in a degradation term, but not in a production term, support oscillations. A new model containing an additional positive feedback loop shows circadian oscillations with the correct phases between the clock components. The phenotype of several clock mutants can be reproduced. The model synchronizes with the light/dark cycle. Assuming restricted light-input (gating), its phase can be fixed to either light onset or light offset with varying day lengths. In this model, the positive feedback has only a minor influence on the robustness of the circadian oscillations towards parameter variations. This explains the rhythmic phenotype of Rev-erb alpha-/- mutant mice that lack a positive feedback. The model can also explain the unexpected regeneration of circadian oscillations in Per2Brdm1/ Cry2-/- double mutant mice. The regeneration of circadian oscillations in the arrhythmic Per2Brdm1 by additional mutation of Rev-erb alpha is predicted. By including Rev-erb alpha explicitly into the model, another negative feedback loop is added: This model reproduces the phenotypes of several clock mutants. Finally, models describing different molecular oscillators and general models with positive or negative feedback loops of varying chain length are compared with respect to their robustness towards parameter variation. The structural design and in particular the kind of feedback underlying the oscillator seems important for the robustness of the model. Further analysis of circadian features with these and other models will give insight into underlying principles of the circadian oscillator.
ElShamy, Wael M. "Developmental requirements of neurotrophins in the mammalian nervous system /." Stockholm, 1998. http://diss.kib.ki.se/search/module/diss.cfm?19980916elsh.
Повний текст джерелаHumphreys, Robyn. "Using a mouse model to understand the effect of hybridization on skeletal and pelage trait variation in mammalian hybrids." Master's thesis, University of Cape Town, 2018. http://hdl.handle.net/11427/29790.
Повний текст джерелаLuo, Ching-Hsing. "A dynamic model of the mammalian ventricular action potential: Formulation and physiological simulations." Case Western Reserve University School of Graduate Studies / OhioLINK, 1991. http://rave.ohiolink.edu/etdc/view?acc_num=case1060102425.
Повний текст джерелаMota, Sílvia Liliana Gomes. "Development of a mammalian cell model to study AIP56." Master's thesis, Universidade de Aveiro, 2011. http://hdl.handle.net/10773/7976.
Повний текст джерелаFish photobacteriose is a bacterial systemic and deadly infection with rapid course and very high mortalities, caused by the Photobacterium damselae subsp. piscicida (Phdp). Phdp spreads through the bloodstream and secretes the apoptogenic exotoxin AIP56 allowing the pathogen to avoid phagocytosis by inducing the apoptotic death of the host phagocytes. Although AIP56 was found to be a key virulence factor of Phdp and the AIP56-related pathology has been well characterized, the molecular targets of the toxin and the molecular pathways it affects/modulates remain to be disclosed. Recent data revealed that AIP56 is an AB-toxin, possessing an N-terminal metalloprotease domain (A domain) responsible for the catalytic activity of the toxin and a C-terminal domain (B domain) involved in the binding/internalization of the toxin into the cells. The N-terminal domain of AIP56 is homologous to the non-LEE encoded effector C (NleC), a type III secreted effector of enteric pathogenic bacteria that cleaves and inactivates the p65 of NF-kB. However, it remains to be investigated if AIP56 also targets NF-kB p65 and if NF-kB p65 inactivation by AIP56 is linked to the apoptogenic activity of the toxin. So far, AIP56 activity has been being studied using an ex vivo model consisting of freshly isolated sea bass peritoneal leukocytes because although the susceptibility of several mammalian cell lines to AIP56 has been assessed, no apoptosis was observed in any of the cell lines tested. This specificity of the toxin may result from the existence of receptors for AIP56 in phagocytes of susceptible hosts and their absence in mammalian cells and suggests that if AIP56 was able to enter mammalian cells, it would be able to induce apoptosis in those cells. The existence of a mammalian cell model for studying in detail the AIP56 activity would be very advantageous, because there is much more availability of tools to study mammalian cells than sea bass cells. In this work, we tested and optimized different protocols for the intracellular delivery of AIP56 and AIP56-related proteins into HeLa cells. We found that the most efficient methods for intracellular toxin delivery were chemical transfection with toxin-encoding expression vectors and the use of the LF/PA system of Bacillus anthracis. The chemical transfection allows not only to obtain and study transiently transfected cells, but also to develop stable transfected cell lines.
A photobacteriose de peixes é uma infecção sistémica de evolução rápida, causada pela bactéria Gram-negativa Photobacterium damselae subsp. piscicida (Phdp), que provoca elevadas mortalidades em várias espécies de peixes marinhos. Nos animais infectados, a Phdp dissemina-se na corrente sanguínea e secreta a AIP56, uma exotoxina apoptogénica que permite ao agente infeccioso escapar à defesa fagocítica do hospedeiro através da indução da morte por apoptose dos fagócitos. Embora tenha sido demonstrado que a AIP56 é um factor-chave da virulência da Phdp e embora a patologia associada à AIP56 esteja bem caracterizada, os alvos moleculares da toxina e as vias moleculares por ela afectadas continuam por desvendar. Dados recentes revelaram que a AIP56 é uma toxina do tipo AB, contendo um domínio N-terminal (domínio A) de metaloprotease responsável pela actividade catalítica e um domínio C-terminal (domínio B) envolvido na ligação e internalização da toxina pelas células. O domínio N-terminal da AIP56 é homólogo ao non-LEE encoded effector C (NleC), um efector presente em várias bactérias patogénicas entéricas e secretado por um sistema de secreção do tipo III. Foi recentemente descrito que o NleC corta e inactiva o p-65 do NF-kB. Contudo, continua por investigar se a AIP56 também tem como alvo o p-65 do NF-kB e se existe uma relação entre um possível corte do p65 e consequente inactivação do NF-kB e a actividade apoptogénica da toxina. Embora se tenha avaliado a susceptibilidade de várias linhas de celulares de mamíferos à AIP56, pelo facto de não se ter observado a ocorrência de apoptose como consequência da exposição à toxina em nenhuma das linhas testadas, até ao momento, a actividade da AIP56 tem vindo a ser estudada usando um modelo ex vivo que consiste em leucócitos peritoneais isolados de robalo. A especificidade observada poderá resultar do facto de os receptores de membrana para a AIP56 existirem nos fagócitos dos hospedeiros susceptíveis e estarem ausentes nas células de mamífero, sugerindo que se a AIP56 entrasse nas células de mamífero, poderia ser capaz de induzir apoptose dessas células. A existência de um modelo celular de mamífero para estudar os detalhes da actividade da AIP56 seria muito vantajosa, pois permitiria utilizar um vasto leque de ferramentas disponíveis comercialmente que não existem ou não podem ser usadas em estudos com células de robalo. Neste trabalho testamos e optimizamos diferentes protocolos para introduzir a AIP56 e proteínas derivadas da AIP56 em células HeLa. Dos resultados obtidos, conclui-se que os métodos mais eficazes para a introdução da toxina foram a transfecção por método químico e a utilização do sistema LF/PA do Bacillus anthracis. A transfecção química permite não só obter e estudar células transfectadas de forma transiente, mas também desenvolver linhas celulares transfectadas estáveis.
Packer, Hans Levi. "A dominant negative over expression model of mammalian MED12 function." Diss., University of Iowa, 2011. https://ir.uiowa.edu/etd/1051.
Повний текст джерелаOpuwari, Chinyerum Sylvia. "Effect of tea and herbal infusions on mammalian reproduction and fertility." Thesis, University of the Western Cape, 2013. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_9358_1380809535.
Повний текст джерелаCamellia sinensis (tea) and Aspalathus linearis (rooibos) may improve reproductive function owing to their antioxidant properties. To test this
hypothesis, male and female rats were given 2% and 5% green tea (Gt), black tea (Bt), unfermented rooibos (Ur) or fermented rooibos (Fr) as sole source of drinking for 52 and 21 days respectively. Control rats received tap water. In addition, TM3 Leydig cells were exposed to 0.025, 0.05, 0.1 and 0.5 % aqueous extracts of green tea, black tea, unfermented and fermented rooibos for 24h. In vitro analysis of tea and the herbal infusion revealed the phenolic property and antioxidant capacity (FRAP) in the order Gt >
Bt >
Ur >
Fr. Camellia sinensis and Aspalathus linearis revealed no significant effect on serum antioxidant capacity (p >
0.05) and lipid peroxidation (MDA) in the kidney or liver in both male and female rats and in the testes of the male rats (p >
0.05). In addition, the antioxidant levels were maintained in the testes, liver and kidneys in both the male and female rats. In the male rats, no significant alterations were observed in body weight gain, liver and reproductive organs weight, and serum testosterone (p >
0.05). Only, 5% green tea significantly increased testosterone level (p <
0.05). Seminiferous tubules displayed complete spermatogenesis with abundant sperm in the lumen in all treated groups. However, a significant decrease in diameter and germinal epithelial height of these tubules were observed (p <
0.05). In the epididymides, epithelial height of caput region showed a significant increase (p <
0.01), while the cauda region was increased by Camellia sinensis but decreased by Aspalathus linearis. Sperm concentration improved significantly by green tea and unfermented rooibos (p <
0.05), while black tea and fermented rooibos produced a non significant effect (p >
0.05). Sperm viability was enhanced in all treatment groups (p <
0.05). Furthermore, green tea, black tea and unfermented rooibos significantly improved the motility of rat sperm (p <
0.05)
fermented rooibos tended to improve it (p >
0.05). In addition, green tea, black tea and fermented rooibos enhanced acrosome reaction (p <
0.05). Creatinine activity was significantly higher in rats treated with black tea, unfermented rooibos or fermented rooibos (p <
0.05), green tea tended to increase it (p >
0.05) reflecting the significant increased kidney weight in the treatment groups at high concentrations. Liver markers, ALT and AST, decreased significantly in all treated groups (p <
0.05), except in 5% fermented rooibos where a significant increase in AST level was observed (p <
0.01). In the female rats, the body weight gain, and reproductive organs weight was no affected (p >
0.05). However, 5% fermented rooibos reduced the ovarian weight (p <
0.05), while 5% unfermented rooibos significantly increased the uterine weight (p <
0.05). Liver weight increased significantly by black tea and unfermented rooibos (p <
0.05) while the kidney weight increased significantly by 5% black tea (p <
0.05). No significant effect was observed in the level of FSH produced, on the other hand, Camellia sinensis significantly lowered the level of LH (p <
0.05), while Aspalathus linearis had no effect (p >
0.05). Creatinine activity was enhanced significantly only by 5% fermented rooibos (p <
0.05). Liver markers, ALT and AST were reduced in most treated groups except in fermented rooibos where an increase was observed. In addition, histological sections revealed no obvious alteration in the ovaries, uteri, kidneys and liver of all treated female rats. Camellia sinensis and Aspalathus linearis significantly reduced the level of testosterone produced in TM3 Leydig cells under stimulated conditions in vitro (p<
0.05). Furthermore, both plants maintained the viability and morphology of the cells. However, at 0.5% of either plant extracts, a significant decrease in the viability (p <
0.05) and altered morphology of the TM3 Leydig cells was observed. In conclusion, Camellia sinensis and Aspalathus linearis significantly improved certain sperm function which might be attributed to their high level of antioxidant activity. However, the prolonged exposure of both plant extracts might result in subtle structural changes in the male reproductive system and impair kidney function. In addition, fermented rooibos at high concentration may also impair the functions of the liver. In vitro, both plants were shown to possess anti-androgenic property on TM3 Leydig cells. Furthermore, both Camellia sinensis and Aspalathus linearis may be classified as weak phytoestrogens due to the changes in the weight of the uterus and ovaries observed.
Elborough, Kieran Michael. "Binding and cleavage of model recombination intermediates by two mammalian proteins." Thesis, Imperial College London, 1990. http://hdl.handle.net/10044/1/46289.
Повний текст джерелаSwift, Rachel D. "Understanding the expression and functions of Lrig3 in a mammalian model." Thesis, University of Oxford, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.601125.
Повний текст джерелаChen, Hui-Zi. "Mammalian Atypical E2Fs Link Endocycle Control to Cancer." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1316540844.
Повний текст джерелаPett, Jan Patrick. "Systems level generation of mammalian circadian rhythms and its connection to liver metabolism." Doctoral thesis, Humboldt-Universität zu Berlin, 2019. http://dx.doi.org/10.18452/19960.
Повний текст джерелаCircadian clocks are endogenous oscillators that generate 24-hour rhythms. They allow many organisms to synchronize their physiology and behaviour with daily changes of the environment. In mammals such clocks are based on transcriptional-translational feedback loops, however, it is not fully understood which feedback loops contribute to rhythm generation. Within an organism different clocks are distinguished by their localization in different organs. One of the key physiological functions of circadian clocks in various organs seems to be the temporal alignment of metabolic processes. In the first project we introduced and applied a method to systematically test regulations in a data-driven mathematical model of the core clock. Surprisingly, we discovered a feedback loop that has previously not been considered in the context of the mammalian circadian clock. This repressilator is consistent with knockout studies and further perturbation experiments. It could constitute an explanation for different phases observed between Cryptochromes, which are part of the core clock. In the second project we repeatedly fitted the same mathematical model to tissue-specific data sets and identified essential feedback loops in all model versions. Interestingly, for all tissue-specific data sets we found synergies of loops generating rhythms together. Further, we found that the synergies differ depending on the tissue. In the third project we examined the circadian aspects of metabolism. We identified rhythmic data in different omics studies, integrated and mapped them to a metabolic network. Our analysis confirmed that many metabolic pathways may follow circadian rhythms. Interestingly, we also found that the average peak times of rhythmic components between various pathways differ. Such differences might reflect a temporal alignment of metabolic functions to the time when they are required.
Tsang, Hiu-Gwen. "Investigating gene expression patterns in the mammalian cardiovascular system." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/31180.
Повний текст джерелаAitken, Sarah Jane. "The pathological and genomic impact of CTCF depletion in mammalian model systems." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/284403.
Повний текст джерелаHarrison, Sarah Ellys. "Utilising embryonic and extra-embryonic stem cells to model early mammalian embryogenesis in vitro." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/275424.
Повний текст джерелаAvva, Jayant. "Complex Systems Biology of Mammalian Cell Cycle Signaling in Cancer." Case Western Reserve University School of Graduate Studies / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=case1295625781.
Повний текст джерелаPatterson, Sean Ingram. "Action of neuroinflammatory mediators on a cell line model for mammalian sensory neurones." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304412.
Повний текст джерелаBussek, Alexandra, Erich Wettwer, Torsten Christ, Horst Lohmann, Patrizia Camelliti, and Ursula Ravens. "Tissue Slices from Adult Mammalian Hearts as a Model for Pharmacological Drug Testing." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-137634.
Повний текст джерелаDieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich
Bussek, Alexandra, Erich Wettwer, Torsten Christ, Horst Lohmann, Patrizia Camelliti, and Ursula Ravens. "Tissue Slices from Adult Mammalian Hearts as a Model for Pharmacological Drug Testing." Karger, 2009. https://tud.qucosa.de/id/qucosa%3A27744.
Повний текст джерелаDieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
Cai, Jingfei. "Probing the Membrane Association Mechanisms for Pulmonary Collectins and Mammalian Phospholipase C." Thesis, Boston College, 2013. http://hdl.handle.net/2345/3872.
Повний текст джерелаThesis advisor: Eranthie Weerapana
Peripheral proteins from mammals often exhibit multi-domain structures and require metal ions such as calcium as co-factors. This dissertation investigates two types of such proteins -- pulmonary collectins (surfactant proteins A and D) and phosphatidylinositol-specific phospholipase C (PLC) delta1 -- and their interactions with model membranes. One approach to work around the complexity brought upon by such multi-domain protein structure is to use a truncated construct or an isolated single domain. For pulmonary collectins, homotrimers consisting of the neck domain and the carbohydrate recognition domain were used in a novel NMR assay for better understanding of their lipid-specific interactions with the membranes. For PLC delta1, we were particularly interested in the role of the EF-hand domain. The isolated EF-hand domain of PLC delta1 was first used to characterize its interactions with membranes and identify key residues responsible for such interactions. These key residues in the N terminal lobe of the EF-hand domain, either cationic or hydrophobic, were then found to affect the hydrolysis activity of the full-length enzyme. A common role for this region of the PLC in facilitating proper membrane association was thus proposed
Thesis (PhD) — Boston College, 2013
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Chemistry
Singh, Geetanjali. "Analysis of genetic mutations using a recombinant model of the mammalian pyruvate dehydrogenase complex." Thesis, Thesis restricted. Connect to e-thesis to view abstract, 2008. http://theses.gla.ac.uk/214/.
Повний текст джерелаPh.D. thesis submitted to the Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, 2008. Includes bibliographical references. Print version also available.
Sidoli, Fabio. "Systematic development of a coupled population-balance-single-cell model for mammalian cell cultures." Thesis, Imperial College London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.428151.
Повний текст джерелаMgwebi, Thandiswa. "Morphological investigations into the development of the mammalian corneal endothelium using the mouse model." Doctoral thesis, University of Cape Town, 2004. http://hdl.handle.net/11427/3268.
Повний текст джерелаThe corneal endothelium (CE), a mesenchyme-derived tissue, is a monolayer of squamous cells on the inner corneal surface. In Foxc1-1• mice, the CE fails to form. The understanding of the cause of this defect has implications for the study of human eye disorders that are related to FOXC1 mutations. To understand the basis of CE defects in Foxc1-1- mice, an analysis of normal CE development was performed using scanning electron microscopy. Results showed that in normal mice the transformation from mesenchyme to endothelium was initiated at embryonic day (E) 12.5 and was characterised by a change from stellate to cobblestone shape and the formation of junctions. In FoxcN- mice, the process was initiated but a cobblestone shape not attained. The expression of adherens (N-cadherin) and tight junction (Z0-1) proteins was investigated by immunoflouresence microscopy. In the normal embryo, the expression of N-cadherin was initially in cytoplasmic vesicles and later at the cell membranes. ZO-l was first detected at the cell peripheries at E13.5. In Foxct-I- mice, N-cadherin peripheral bands failed to form. ZO-l was not expressed. These results suggest that the failure to form a monolayered CE in Foxc1 mice is due to incomplete mesenchyme-endothelial conversion. Junction formation was further investigated in vitro. N-cadherin was cytoplasmic in pre-confluent cells and at cell edges in confluent cells. ZO-l was not detected. These results suggest that in vitro, these cells are either unable to form tight junctions or the culture medium does not contain the appropriate signalling molecules.
Sankaran, Saumya M. "A functional analysis of the mammalian E3 ubiquitin ligase WWP1 in a yeast model." Waltham, Mass. : Brandeis University, 2009. http://dcoll.brandeis.edu/handle/10192/23252.
Повний текст джерелаConzelmann, Holger. "Mathematical modeling of biochemical signal transduction pathways in mammalian cells a domain-oriented approach to reduce combinatorial complexity /." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-38819.
Повний текст джерелаKabir, Mitra. "Prediction of mammalian essential genes based on sequence and functional features." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/prediction-of-mammalian-essential-genes-based-on-sequence-and-functional-features(cf8eeed5-c2b3-47c3-9a8f-2cc290c90d56).html.
Повний текст джерелаMaree, Liana. "Sperm mitochondria: Species specificity and relationships to sperm morphometric features and sperm function in selected mammalian species." Thesis, University of the Western Cape, 2011. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_1728_1363788268.
Повний текст джерелаNumerous studies on mammalian spermatozoa have reported large variations in the dimensions of the main sperm structural components, namely the head, midpiece and flagellum. These variations in sperm architecture are believed to be adaptations for functioning of spermatozoa in complex environments outside the male reproductive system. The midpiece of the mammalian 
permatozoon contains a varied number of mitochondria, but the reason for the marked difference in the size and structure of this sperm component is not clear. This study 
confirmed the variations in the sperm morphometry of seven selected mammalian species and revealed unique features of the sperm midpiece and sperm mitochondria of these seven species. Evaluation of several sperm kinematic parameters revealed the unique swimming characteristics of the different spermatozoa. The importance of using standardized motility 
parameters was highlighted as well as the assessment of different subpopulations of spermatozoa in order to produce more reliable and comparable data. Investigating the role of sperm mitochondria in human sperm 
metabolism indicated that these organelles are related to sperm function in terms of sperm motility. Furthermore, it was suggested that glycolysis and mitochondrial respiration are linked processes and that both are important for the maintenance of human sperm motility. By optimizing and employing standardized experimental procedures and analysis techniques, this study was 
able to confirm the species specificity of almost all the sperm parameters evaluated, while also elucidating the phylogenetic relatedness of the non-human primate species. In conclusion, the present study has confirmed that the various midpiece morphometry parameters are related to the remaining sperm morphometry parameters as well as to the sperm kinematic parameters. 
These proposed associations between the various sperm parameters were used to explain the sperm velocity of two hypothetical and morphologically different sperm structures. Therefore, the results of the current study support the idea of co-evolution between sperm components in mammalian spermatozoa and propose that the midpiece morphometry parameters that are selected for in these spermatozoa are midpiece volume, total number of mitochondrial gyres, thickness of the mitochondrial sheath and mitochondrial height.
Hanon, Elodie. "A novel model of action for TSH (thyrotropin stimulating hormone) in the mammalian neuroendocrine system." Thesis, Available from the University of Aberdeen Library and Historic Collections Digital Resources, 2009. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=53386.
Повний текст джерелаPermana, Paskasari A. "SV40 minichromosome: A mammalian replicon model for investigations of antineoplastic drugs and DNA damaging agents /." The Ohio State University, 1993. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487846885776862.
Повний текст джерела