Дисертації з теми "M. tuberculosis Infection"
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Du, Toit Eben Francois. "Modelling the co-infection dynamics of HIV-1 and M. tuberculosis." Diss., Pretoria : [s.n.], 2008. http://upetd.up.ac.za/thesis/available/etd-08172008-213855.
Повний текст джерелаChiacchiaretta, Matteo. "M. tuberculosis lineages: genetic diversity and its involvement on macrophage infection and on drug tolerance." Doctoral thesis, Università di Siena, 2022. http://hdl.handle.net/11365/1183830.
Повний текст джерелаKieswetter, Nathan Scott. "Remodelling of Mycobacterial Peptidoglycan During Cell Division and the Epigenetics of Macrophages during M. tuberculosis infection." Doctoral thesis, Faculty of Health Sciences, 2021. http://hdl.handle.net/11427/33815.
Повний текст джерелаRoy, Eleanor. "Response of dendritic cells to Mycobacterium tuberculosis infection and the induction of protective immunity using dendritic cells infected with an auxotrophic mutant of M. tuberculosis." Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1446801/.
Повний текст джерелаAdankwah, Ernest [Verfasser]. "Pathognomonic effects of human tuberculosis on host immune response in an endemic population: impact on T-cell functions and M. tuberculosis infection diagnosis / Ernest Adankwah." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2020. http://d-nb.info/1213094801/34.
Повний текст джерелаNeumann, Jan [Verfasser]. "Characterization of macrophage Frizzled1 expression and the role of Wnt3a-induced signaling in experimental M. tuberculosis infection / Jan Neumann." Lübeck : Zentrale Hochschulbibliothek Lübeck, 2010. http://d-nb.info/1004898347/34.
Повний текст джерелаWyndham-Thomas, Chloe. "Screening for latent M. tuberculosis infection in HIV-positive patients residing in low tuberculosis incidence settings: Investigation of the current practices and identification of clinical- and immune-based strategies for improvement." Doctoral thesis, Universite Libre de Bruxelles, 2016. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/241270.
Повний текст джерелаDoctorat en Sciences médicales (Médecine)
info:eu-repo/semantics/nonPublished
COLONE, ALESSIA. "Role of CpG ODNs in human macrophages before and after infection with Mycobacterium tuberculosis and their effects in cellular and molecular defence mechanisms." Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2010. http://hdl.handle.net/2108/1265.
Повний текст джерелаCarmo, Ana Maria do. "Modulação da resposta imunológica no pulmão de Camundongos co-infectados com Mycobacterium bovis e Strongyloides venezuelensis." Universidade Federal de Juiz de Fora (UFJF), 2008. https://repositorio.ufjf.br/jspui/handle/ufjf/2989.
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Sabe-se que existem inúmeros trabalhos envolvendo a modulação da resposta imune ao Mycobacterium. No entanto, o número de indivíduos apresentando tuberculose é cada vez maior. A resposta imune ao Mycobacterium é desencadeada principalmente por linfócitos Th1, com a produção de IFN-γ. As parasitoses intestinais também representam um importante problema médico-sanitário, tendo em vista o grande número de pessoas acometidas e as inúmeras alterações orgânicas que podem provocar no hospedeiro. Essas infecções helmínticas induzem preferencialmente uma resposta Th2 com a produção de IL-4, IL-5 e IL-13. Este trabalho avaliou a regulação da resposta imune no pulmão de camundongos co-infectados ou não por S. venezuelensis (SV) e/ou Mycobacterium bovis-BCG (MB), em dois pontos das duas infecções, denominados como ponto 1 (4° e 7° dia pós-imunização [dpi]) e ponto 2 (7° e 10° dpi) por MB e SV, respectivamente. Os animais foram infectados com 700 larvas de SV pela via subcutânea e, após 3 dias, com 1x106 UFC de MB cepa selvagem pela via intravenosa. Realizou-se a quantificação do número de ovos e vermes, a dosagem de citocinas (IFN-γ, IL-4 e IL-10) e quimiocinas (CCL2 e CCL5), o envolvimento de MPO e EPO, a detecção da infecção pelo MB por PCR, a avaliação histopatológica e a expressão de moléculas coestimulat órias/imunomodulatórias (CD80, CD86, CD28, CTLA-4 e CD25) em células ou tecidos do pulmão dos animais infectados e/ou co-infectados. Os resultados mostraram que a presença do MB favoreceu para o aumento do número de ovos e vermes do SV observados nos animais nos dias 4° e 7° (ponto 1) e 7° e 10° (ponto 2) após a infecção por MB e SV, respectivamente, nos animais co-infectados (COIN). A reação de PCR foi efetiva em detectar a presença do MB no pulmão dos animais. Foi observado um aumento de IFN-γ e uma diminuição de IL-4 e EPO no pulmão dos animais do grupo COIN, além de aumento na expressão da molécula co-estimulatória CD80 no ponto 1 e uma diminuição no ponto 2. Houve uma alta produção de IL-10 no pulmão dos animais dos grupos MB e COIN, sendo que a histopatologia neste sítio mostrou formação de granulomas com grande influxo de neutrófilos, macrófagos e células epitelóides na periferia nos pulmões dos animais do grupo MB e um granuloma bem mais avançado, com centro necrótico nos animais do grupo COIN. Baseado nesses resultados, conclui-se que o MB modula a infecção pelo SV, fazendo com que os animais fiquem mais suscetíveis à infecção helmíntica. Por outro lado, o SV modula a infecção pelo MB, fazendo com haja uma modificação na formação de granuloma no pulmão dos animais do grupo COIN no ponto 1 da infecção pelo MB, que poderia ser justificada pela diminuição de IL-4 nos animais do grupo COIN.
A rising number of people have been contracting tuberculosis around the world even though a multitude of reports involving a modulation of the immune response to Mycobacterium have been published. The response to Mycobacterium is mainly mediated by Th1 lymphocytes through IFN-gamma production. Parasitic diseases account for a large proportion of human morbidity and mortality, considering the number of people affected by them and several pathologies associated to parasitic infection. Helminthic infections drive towards Th2 response which leads to IL-4, IL5 and IL-13 production. The present study evaluated the immune response of coinfected animals or not with Strongyloides venezuelensis (SV) and Mycobacterium bovis-BCG (MB) on pulmonary cells collected from BALB/c mice at time points 1 (4th and 7th days post-immunization [dpi] by MB and SV, respectively) and 2 (7th and 10th dpi by MB and SV, respectively). Animals were infected with 700 SV larvae subcutaneously, and 3 days after, 1x106 CFU of wild MB strain intravenously. The number of worms and eggs was counted as well as cytokine (IFN-gamma, IL-4 and IL-10) and chemokine (CCL2 and CCL5) assessments, and the MPO and EPO levels determination on pulmonary tissue from infected and/or coinfected animals. In addition, PCR for MB detection, the histopathology and the expression of costimulatory molecules such as CD80, CD86, CD28, CTLA-4 and CD25 on pulmonary tissue were also assessed. The results pointed that MB led to increase SV parasite burden in coinfected mice (COIN) at both time points analyzed. The PCR technique detected effectively MB. Moreover, elevated IFN-gamma and reduced IL-4 and EPO levels were detected on pulmonary tissue in the COIN group. In regard to CD80 molecule, there was an increased expression at time point 1 and diminished expression at time point 2. Also, higher amounts of IL-10 were found on pulmonary tissue in MB and COIN groups. The histopathological analysis revealed pulmonary granulomas with a number of neutrophils, macrophages and epithelial cells-like in the MB group as well as granulomas in an advanced stage with caseous necrosis in the COIN group. Based on these findings, it may be concluded that MB modulated the immune response to SV, leading coinfected animals to be more susceptible to helminthic infection. On the other hand, SV modulated the MB infection by modifying the characteristics of the pulmonary granulomas in the COIN group at time point 1 probably due the reduced IL-4 production in this group.
Lopes, Fernando Henrique Azevedo. "NÃveis sÃricos de interleucina-6 e polimorfismo - 174G>C em infecÃÃo latente pelo Mycobacterium tuberculosis." Universidade Federal do CearÃ, 2012. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=7533.
Повний текст джерелаA interleucina-6 (IL-6) à uma importante citocina que exerce papel fundamental na imunopatogÃnese de diversas doenÃas infecciosas. O objetivo deste estudo foi investigar o nÃvel de produÃÃo sistÃmica de IL-6 e aferir o papel funcional do polimorfismo -174 G>C do gene dessa citocina em indivÃduos diagnosticados como portadores de infecÃÃo latente pelo Mycobacterium tuberculosis (ILTB). Para controle, foram utilizados dois grupos de comparaÃÃo: um deles composto por portadores de tuberculose pulmonar ativa (TB) e o outro formado por indivÃduos saudÃveis, doadores de sangue. O grupo ILTB foi composto por 15 indivÃduos, selecionados dentre os contactantes de portadores de TB pulmonar ativa, atendidos no Hospital SÃo Josà de DoenÃas Infecciosas e no Centro de SaÃde da FamÃlia AnastÃcio MagalhÃes. O grupo TB foi formado por 38 pacientes com TB pulmonar ativa, procedentes do Hospital de Messejana, Hospital de Maracanaà e Hospital Geral Dr. CÃsar Cals. O grupo de indivÃduos saudÃveis contava com 63 doadores voluntÃrios de sangue do Centro de Hematologia e Hemoterapia do CearÃ. A dosagem sÃrica de IL-6 foi realizada por meio de um ensaio imunoenzimÃtico (ELISA), com kit especÃfico fornecido pela Invitrogen Corporation. Para purificaÃÃo do DNA, foi utilizado o kit GFX Genomic Blood DNA Purification, da GE Healthcare. O polimorfismo -174GC do gene da IL â 6 foi tipificado pela tÃcnica de reaÃÃo em cadeia da polimerase (PCR), utilizando-se iniciadores de sequÃncia especÃfica (PCR-SSP) (One-Lambda). As medianas de concentraÃÃes sÃricas de IL-6 para os grupos ILTB, TB e saudÃveis foram de, respectivamente, 1,7 pg/mL, 4,3 pg/mL e 0,5 pg/mL (p < 0,0001). Nos trÃs grupos estudados, o genÃtipo encontrado com maior frequÃncia foi o G/G [ILTB = (80%); TB = (58,9%); saudÃveis = (62,8%)]. Em conclusÃo, podemos inferir que a IL-6 deve desempenhar um papel importante na manutenÃÃo do estado de latÃncia, haja vista que sua concentraÃÃo, nos indivÃduos com ILTB, foi 3,4 vezes maior que no grupo saudÃvel. Ademais, constatamos que, na populaÃÃo estudada, o polimorfismo -174GC nÃo se mostrou funcional no Ãmbito da infecÃÃo latente pelo Mycobacterium tuberculosis.
Interleukin-6 (IL-6) is an important cytokine involved in the pathogenesis of multiple infectious diseases. The aim of this study was to investigate the levels of IL-6 production and to correlate to the -174G>C polymorphism at the IL-6 gene in latent infection with M. tuberculosis (ILTB). As controls, two groups were used. One of them with active pulmonary tuberculosis (TB) patients and the other with healthy blood donors. ILTB group was composed by 15 individuals, selected among active pulmonary TB contacts seen at the Hospital SÃo Josà de DoenÃas Infecciosas and the Centro de SaÃde da FamÃlia AnastÃcio MagalhÃes. TB group had 38 patients with active pulmonary disease seen at the Hospital de Messejana, Hospital de Maracanaà and the Hospital Geral Dr. CÃsar Cals. The third group was composed by 63 healthy blood donors from the Centro de Hematologia e Hemoterapia do CearÃ. Serum levels of IL-6 were measured by an ELISA using specific kits from Invitrogen Corporation. For DNA purification a GFX Genomic Blood DNA Purification kit (GE Healthcare) was used. The -174GC polymorphism was analyzed by a SSP-PCR method using One-Lambda kits. Median values of serum levels of IL-6 from ILTB, TB and healthy groups were, respectively, 1.7 pg/mL, 4.3 pg/mL and 0.5 pg/mL (p < 0.0001). For the three studied group, the most frequent genotype found was the G/G (ILTB = 80%; TB = 58.9%; saudÃveis = 62.8%). In conclusion, it is possible to consider that IL-6 should play an important role in the maintenance of latent infection state as its concentrations were 3.4 fold higher in ILTB group than that of healthy controls. Moreover, the -174GC polymorpism was not functional in the ILTB group.
Chia, Mi-Yuan, and 賈敏原. "Diagnosis of Mycobacterium tuberculosis、M. bovis、M. avium subsp. avium and M. avium subsp. paratuberculosis infection by Multiplex-PCR." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/37238988103658852777.
Повний текст джерела國立臺灣大學
獸醫學研究所
89
The 4 major pathogenic mycobacteria, M. tuberculosis, M. bovis, M. avium subsp. avium, and M. avium subsp. paratuberculosis, have been identified in Taiwan for many years, remaining important for public health and animal production. The characteristics of the 4 mycobacteria are very similar and it is difficult to differentiate them clinically and histopathologically. The conventional diagnosis for animal mycobacterial infection include PPD, bacterial culture, and acid-fast staining etc. However, these methods have innate defects that may sometimes cause perplexity in the diagnosis. The molecular biotechnologies have been widely used in disease diagnosis. The polymerase chain reaction (PCR) has become a new tool for a more rapid and accurate diagnosis of tuberculosis and paratuberculosis. The objective of the present study was to establish a multiplex-PCR for the diagnosis and differentiation of the 4 major pathogenic mycobacteria simultaneously. The primers and DNA probes were selected and designed according to other’s publication and gene bank comparison, respectively. A two-step multiplex-PCR using 5 pairs of primer was established with standard bacteria. In the first step, 3 pairs of primers were included; primer GroEL amplified a 383 bp nucleotide fragment encoding for the GroEL gene of Mycobacterium spp; primer PT1-2 amplified a 396 bp nucleotide fragment encoding for the mtp40 gene of M. tuberculosis; primer JB21-22 amplified a 495 bp nucleotide fragment of M. bovis. In the second step, 2 pairs of primer were included; primer IS1245 amplified a 427 bp nucleotide fragment encoding for the IS1245 gene of M. avium subsp. avium; primer IS900 amplified a 229 bp nucleotide fragment encoding for the IS900 gene of M. avium subsp. paratuberculosis. Formalin-fixed and paraffin-embedded (FP) tissues from various animal species and samples of blood, milk, nasal discharge, and feces from PPD positive and negative cows were then tested by this method. When the result of multiplex-PCR was obscure, southern blotting was used for further confirmation. For the FP tissues, the 1 M. tuberculosis and 7 M. avium subsp. paratuberculosis cases diagnosed by bacterial culture and/or histopathological examination were all positive by the multiplex-PCR. There were only 4/7 cases of M. bovis-infected PPD-positive cows were positive due to insufficient tissues in the blocks or lack of acid-fast bacteria in the tissue. For other 28 tuberculosis-speculated cases by pathology or PPD test, 8/9 cases and 1/9 case were multiplex-PCR negative due to lack of enough tissues and possibly DNA breakdown, respectively; the remaining 12/19 and 5/19 cases were M. bovis and Mycobacterium spp. positive by multiplex-PCR, respectively; and 2/19 cases (F91-129 and F90238) were multiplex-PCR negative although 1 was acid-fast positive and both had sufficient tissues in the blocks. Tissues from 5 cases suspected having M. avium subsp. avium infection pathologically were all negative by multiplex-PCR. It was possible that primer IS1245 is not specific for all the serotypes of M. avium subsp. avium; additionally, M. avium subsp. intracellulare is very similar to M. avium subsp. avium clinically and histopathologically and primer IS1245 can not detect M. avium subsp. intracellulare. The feces and nasal discharge from PPD positive and negative cows were all negative by multiplex-PCR. For the blood and milk, 34/57 blood samples were M. bovis positive by multiplex-PCR within which 26 were PPD-positive; there were also 7 PPD-positive cases were multiplex-PCR negative. Four out of 13 milk samples were M. bovis positive by multiplex-PCR; blood samples from the 4 cases were M, bovis positive and 3 of them were PPD-positive as well. Besides, one milk case was PPD-positive but both blood and milk were multiplex-PCR negative. It is believed that PPD test combined with blood multiplex-PCR will improve the detection of mycobacterial infection more effectively.
"CD4-Independent Correlates of Protection in M. tuberculosis and Mtb/SIV Co-Infection." Tulane University, 2018.
Знайти повний текст джерелаIn order to develop better therapeutics and treatment strategies for tuberculosis (TB) infection, it is imperative to understand interactions that occur in the host in response to the bacilli that contribute to disease progression. Modeling of TB in simple animal models such as mice and zebrafish is often incomplete as there are evolutionary differences, as well as structural issues, that reduce the faithfulness to human TB infection. The non-human primate model of TB infection provides the added benefit of providing a long-established model of SIV infection that recapitulates HIV infection in humans and has been expanded to model TB/HIV co-infection. Here, we have sought to identify correlates of protection in TB and TB/SIV co-infection using rhesus macaques. In the first experiment, we used two strains of Mycobacterium tuberculosis (Mtb), CDC1551 and Erdman, to investigate strain-specific mechanisms of virulence. As increased virulence of Mtb Erdman was associated with excess inflammatory responses, we sought to evaluate a host-directed therapeutic in a lethal challenge model of Mtb CDC1551 infection. We found that use of a type I interferon antagonist significantly improved host survival in the absence of antibiotic treatment and survival was associated with the presence of increased levels of granzyme B producing T cells. A major producer of granzyme B, mucosal-associated invariant T (MAIT) cells was investigated in Mtb/SIV co-infection, but was found to not contribute to protection in TB or TB/SIV. In order to further expand our model of Mtb/SIV co-infection, we co-infected latent Mtb-infected rhesus macaques with a non-pathogenic strain of SIV, SIVmac239ΔGY, and administered a CD4-depleting antibody, CD4R1, in place of SIVmac239 co-infection. Using SIVΔGY, we found that virulent viral replication was necessary for TB reactivation. Using both SIVΔGY and antibody-mediated CD4+ T cell depletion, we found that immune responses are disregulated in Mtb/SIV reactivators in a divergent manner, illustrating the presence of SIV-dependent factors that contribute to TB/SIV reactivation. Overall, these results indicate that immune mechanisms, especially those of inflammation, are significant in determining host outcomes. Developing ways to better control inflammation are necessary to supplement antibiotic treatment and cure TB.
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Allison Bucsan
Bharadwaj, Vemparala. "Unraveling the Evolutionary Advantages of Crosstalk Between Two-Component Signalling Systems of M tuberculosis." Thesis, 2017. http://etd.iisc.ac.in/handle/2005/3636.
Повний текст джерелаBharadwaj, Vemparala. "Unraveling the Evolutionary Advantages of Crosstalk Between Two-Component Signalling Systems of M tuberculosis." Thesis, 2017. http://etd.iisc.ernet.in/2005/3636.
Повний текст джерелаMishra, Archita. "Pranlukast as an Allosteric Inhibitor of M.Tuberculosis Ornithine Acetyltransferase : Implication Towards Novel Combinatorial Therapy." Thesis, 2017. http://etd.iisc.ac.in/handle/2005/4171.
Повний текст джерелаLiu, Shu-feng, and 劉淑鳳. "The epidemiologic comparison of M. avium complex with M. tuberculosis complex and genetic analysis of Rifampicin resistance for M. avium complex of the Infection Disease Hospital in Kaohsiung and Pingtung areas." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/93792242147031238964.
Повний текст джерела嘉南藥理科技大學
生物科技系暨研究所
98
Abstract Mycobacterium avium complex (MAC) belongs to a group of slow growing mycobacteria. Many studies showed that chronic pulmonary disease is most commonly manifestated by a non-tuberculous mycobacteria (NTM) infection. MAC, M. kansasii, and M. abscessus, in order of frequency, are the most common pathogens of NTM lung disease. We found the patients with NTM lung disease were more than those with pulmonary tuberculosis between July, 2007 and December, 2008 and MAC was the most common pathogen of NTM lung disease. A case-control was conducted study and 30 patients with a positive sputum culture for MAC as the case group and 30 patients with a positive sputum culture for M. tuberculosis as the control group were enrolled. The two groups were matched with sex, residential areas, and patient services (outpatients or inpatients). Several variables as predictors of risk factors were used to evaluate acquiring NTM lung disease, including age, height, weight﹐BMI﹐DM, smoking, upper lung fields﹐lower lung fields, and pulmonary cavities. We also compared the gene sequence by sequencing to identify the Rifampicin resistance gene of MAC. Involvement of lower lung fields were more common in MAC lung disease (McNemar test, p <0.05), while the pulmonary cavities were more common in pulmonary tuberculosis (Logistic regression, p <0.05). Finally, EMBOSS were used for the database of M.avium AF060366 and sequence analysis. Three strains with a point mutation (Glycine→Aspartic acid) in the nucleotide sequence 433 was noted. Twenty-six strains (87%) presented the phenotype of Rifampicin resistance, while 4 strains were sensitive to Rifampicin (13%). However, genotypes of Rifampicin resistance were noted for only 11% of the strains. It implies that a new criteria for clinical susceptibility test should be established to provide a more convenient, cheap and accurate method for the in-vitro susceptibility test.
Dlamini, Mcebo Edwin Maswati. "The prevalence of bovine tuberculosis and associated risk factors for humans in Swaziland." Diss., 2013. http://hdl.handle.net/2263/36811.
Повний текст джерелаDissertation (MSc)--University of Pretoria, 2013.
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Veterinary Tropical Diseases
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