Добірка наукової літератури з теми "Lysteria Monocytogenes"

Experimental infections of guinea pigs and rabbits with a sublethal dose of L. monocytogenes pathogen caused an allergic reaction in the form of delayed-type hypersensitivity (DTHS), which was detected by an intradermal allergy test with a Listeriose allergen. The status of DTHS in guinea pigs and rabbits was recorded for a longer time as compared to specific antibodies. A positive allergic reaction correlated with listeria, which was confirmed by the isolation of a Listeria culture 6 months after infection of rabbits with a virulent Listeria strain. The research findings showed that an intradermal allergy test with a developed Listeria allergen allows a retrospective diagnosis of Listeriosis and Listeria carrying. A specific feature of Listeria allergen was established through an intradermal provocative test in animals sensitized by heterogeneous microorganisms (Salmonella and E. coli).

We report the case of a 48-year-old woman, with a rapidly progressing ACTH neuroendocrine tumour of the pancreas (PNET) and multiple liver metastases. The patient had previously suffered from a peptic ulcer which was responsive to PPI inhibitors and hypertension which was poorly controlled by therapy. Admitted to the hospital for severe asthenia and abdominal pain, she was diagnosed with poorly differentiated PNET with liver metastases, which were positive for synaptophysin, cytokeratin 7 and 9 and neuron specific enolase (NSE). Octreoscan scintigraphy was positive for somatostatin receptors in the pancreas and in two liver lesions. A rapidly progressive Cushing’s syndrome developed, presenting with the classical physical symptoms, hypokalemia and Lysteria monocytogenes meningitis. Ectopic ACTH production was confirmed and eventually the patient died from a septic shock within two months. The case reported focuses on the malignity and the rapid progression of an ACTH-producing PNET and calls attention to the possible fatal progression of these cases.

The study investigates four paper-based materials designed for short-time wrapping of meat products by determining morpho-structure, capillary-hydroscopic, barrier and antibacterial properties, wettability and migration into food simulants. The paper-based materials are coded as RO, SP, IT and SLO. RO and SLO samples exhibit the best barrier properties against water vapors. The low solubility and contact angles of RO, IT and SLO in A simulant (distilled water) make them suitable for aqueous food storage. The extremely high solubility of SP and SLO in simulant B (acetic acid) shows that wax and hydrophobized starch, respectively are carried by the acidic media, thus these agents are unlikely to coat the paper designed to package acidic food. SLO inhibits E. coli, Salmonella enterica, Lysteria monocytogenes, Pseudomonas aeruginosa and fluorescens. Polyethylene coated on RO and IT surface and wax impregnated on SP have a lower antimicrobial activity in comparison with hydrophobized starch coated on SLO.

Natural extracts (rich in bioactive compounds) that can be obtained from the leaves, peels and seeds, such as the studied extracts of Pomegranate (P), Rosemary (RA, Nutrox OS (NOS) and Nutrox OVS (NOVS)), and olive (Olea europaea) extracts rich in hydroxytyrosol (HYT-F from olive fruit and HYT-L from olive leaf) can act as antioxidant and antimicrobial agents in food products to replace synthetic additives. The total phenolic compounds, antioxidant capacity (measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-Azinobis (3-ethylbenzothiazolin) -6-sulphonic acid (ABTS), Ferric Reducing Antioxidant Power (FRAP), and Oxygen Radical Absorbance Capacity (ORACH)) and their antimicrobial power (using the diffusion disk method with the Escherichia Coli, Lysteria monocytogenes, and Staphilococcus Aureus strains) were measured. The results showed that all the extracts were good antioxidant and antimicrobial compounds in vitro. On the other hand, their antioxidant and antimicrobial capacity was also measured in fish products acting as preservative agents. For that, volatile fatty acid compounds were analysed by GS-MS at day 0 and 11 from elaboration, together with total vial count (TVC), total coliform count (TCC), E. Coli, and L. monocytogenes content at day 0, 4, 7 and 11 under refrigerated storage. The fish patties suffered rapid lipid oxidation and odour and flavour spoilage associated with slight rancidity. Natural extracts from pomegranate, rosemary, and hydroxytyrosol delayed the lipid oxidation, measured as volatile compounds, and the microbiological spoilage in fish patties. Addition of natural extracts to fish products contributed to extend the shelf life of fish under retail display conditions.

Backgroundjuvenile systemic lupus erythematosus (jSLE) is the most frequent pediatric connective tissue disease with multiorgan involvement and different outcomes and prognosis. Corticosteroids remain the base treatment option and steroid-sparing treatment is strongly required to avoid steroid toxicity. Rituximab (RTX) is one of biologics, which efficacy was proved in case reports and case series of SLE, but no data from big randomized trials, confirming the efficacy have existed.Objectivesto evaluate safety and efficiency of RTX in jSLE.Methodsin the retrospective observation study the information of 48 jSLE patients (12 boys, 36 girls) who received at least one RTX dose before 18 years, included. Diagnosis was made using SLICC criteria. The main indications for RTX were high disease activity with lupus nephritis (LN), CNS and hematology disturbances (hemolytic anemia, thrombocytopenia) and avoiding steroid toxicity. RTX was prescribed in dosage 375 mg/m2 every week (2-4 infusions) with repeated courses every 6-12 months according disease activity, the degree of B-cell depletion and hypoIgG-emia. The dynamics of clinical, laboratory data, activity of the disease by SLEDAI, GCS doses were assessed in the onset and during RTX trial.ResultsThe main patient’s characteristics were: onset age 13.0 (11.5; 15.0) years, inclusion age 18.0 (16.0; 20.0) years, LN 25 (52%)/III+IV class 9/11 (82%), CNS involvement 26 (54%). Pre-RTX non-biologic conventional treatment includes: cyclophosphamide 24 (50%), MMF 14 (29%), azathyoprine 7 (15%), methotrexate 6 (13%), cyclosporine A 2 (4%). Observation period ranged from 6 months to 6 years with median time 0.75 (0.2; 2.75) years. Initial pre-RTX treatment (GCS, hydroxychloroquine, non-biologic DMARDS) partially reduced SLE activity (SLEDAI, ANA titer, anti-dsDNA level), and median GCS dose by 25% from the initial dose, without changes in proteinuria, hematuria, C3, C4, WBC, hemoglobin, PLT and ESR levels. Administration of the RTX realized in prominent reducing of SLEDAI, anti-dsDNA level, proteinuria, hematuria, C4, ESR, number of patients with anemia, thrombocytopenia, and median GCS dose by 90% from the initial. The hemoglobin level and WBC have increased. 19 patients received IVIG for treatment of MAS (n=3), infection (n=5) and as replacement treatment in cases where IgG<4.5 g/l (n=11). 3 deaths were observed due to catastrophic SLE with MAS, accompanied severe infection (invasive aspergillosis, n=2). 6 patients realized SAE: pneumonia (n=3), transient agranulocytosis (n=1) after 3rd RTX infusion and meningitis, caused by Lysteria monocytogenis, after 1st RTX infusion (further RTX treatment continued without adverse events), patella osteomyelitis (n=1). 10 patients received antibiotics for respiratory infections. On pre-RTX 13 had antibiotics (p=1.0).Table 1.Dynamics of SLE features pre-RTX and during RTX trialParameterSLE onsetRTX (baseline)pLast visitp*SLEDAI16 (11.0; 23.5)13.5 (6.5; 21.5)0.00024 (0; 8.0)0.00002Patients with elevated anti-dsDNA n, (%)33 (69)20 (42)0.00811 (23)0.034Anti-dsDNA, U/ml (n.v.<25)112 (1; 200)24.7 (1; 130)0.0590 (0; 27)0.008Proteinuria, g/l2.6 (0.8; 4.4)3.8 (0.3; 7.5)0.6870 (0; 0.2)0.004Hematuria, # cells40 (8; 86)50 (6; 120)0.1910 (0; 1)0.0016C4, g/l0.12 (0.1; 0.24)0.12 (0.06; 0.19)0.3980.15 (0.11; 0.21)0.016Patients with leucopenia, n(%)12 (25)10 (21)0.6295 (10)0.00001Patients with anemia n (%)19 (40)16 (33)0.097 (15)0.0015Hemoglobin, g/l113 (95;131)115 (91; 132)0.830128 (107; 134)0.063Patients with thrombocytopenia n (%)17 (35)9 (19)0.0052 (4)0.00001ESR, mm/h17 (8; 31)15 (7; 22)0.1347 (2; 20)0.054Patients with GCS therapy n, (%)45 (94)45 (94)1.040 (83)0.00001GCS, mg/kg1.0 (0.6; 1.0)0.75 (0.2; 1.0)0.0350.1 (0.08; 0.28)0.000001*compare to RTX baselineConclusionRTX showed effectiveness in the cases, where previous non-biologic treatment was insufficiently effective. Randomized controlled trials are required to evaluate the efficacy and safety of RTX.AcknowledgementsThis research was funded by the Ministry of Science and Higher Education of the Russian Federation (Agreement No. 075-15-2020-901)Disclosure of InterestsNone declared

Penelitian ini bertujuan mengetahui pengaruh ekstrak etanol biji jintan hitam (EEBJH) terhadap aktivitas fagositosis dan sekresi reactive oxygen intermediates (ROI) makrofag peritoneal mencit Swiss yang diinfeksi Lysteria monocytogenes (L. monoytogenes). Dalam penelitian ini digunakan 72 ekor mencit jantan galur Swiss dengan berat antara 20-30 g. Mencit dibagi ke dalam enam kelompok, masing-masing terdiri atas 12 ekor. Kelompok 1 (kelompok kontrol negatif), diberi akuades secara per oral. Kelompok II (kelompok kontrol positif), hewan uji diberi imboost per oral. Kelompok III, IV, V, dan VI sebagai kelompok perlakuan, masing-masing diberi EEBJH dengan dosis 1, 5, 25, dan 125 mg/kg bobot badan/hari per oral selama 14 hari. Pada hari ke-15, semua mencit diinfeksi L. monocytogenes. Aktivitas fagositosis makrofag peritoneal diamati dengan metode lateks sedangkan aktivitas sekresi ROI diamati dengan metode nitro blue tetrazolium (NBT) reduction assay. Hasil penelitian menunjukkan bahwa pemberian EEBJH meningkatkan aktivitas fagositosis dan sekresi ROI makrofag peritoneal yang diinfeksi L. monocytogenes. Angka kematian hewan uji pada kelompok perlakuan lebih rendah dari kelompok negatif. Aktivitas fagositosis dan sekresi ROI makrofag tertinggi terdapat pada hari ke-14. Berdasarkan hasil penelitian maka dapat disimpulkan bahwa EEBJH memiliki efek meningkatkan aktivitas fagositosis dan sekresi ROI makrofag mencit Swiss yangdiinfeksi L. monocytogenes. Kelompok perlakuan dengan dosis 5 mg/kg bobot badan EEBJH memiliki aktivitas fagositosis dan sekresi ROI tertinggi.

Listeria monocytogenes is a pathogenic, psychrotrophic microorganism that is ubiquitous in nature. L. monocytogenes has been isolated from numerous meat products, both fresh and processed, the incidence of contamination varying greatly. The ability of Listeria to grow in meats depends on temperature, pH, water activity (a$ sb{ rm w}$), nutrients, species and numbers of competing microorganisms, gaseous conditions, and levels of additional barriers. Therefore, methods to control the growth of L.monocytogenes are of great importance to food processors since this organism can grow under a wide range of environmental and storage conditions. Two methods of control, in conjunction with temperature, were studied in this project: (i) modified atmosphere packaging (MAP) and (ii) chitosan, to determine the optimum levels of these "hurdles" needed to effectively control the outgrowth of L.monocytogenes in both model broth and agar systems and in fresh pork loin. On the basis of these preliminary studies, a combination of chitosan as a dipping solution and modified atmosphere packaging were investigated to control the growth of L.monocytogenes in fresh pork loin. Pork loin samples were dipped in a 0.2% chitosan solution for 60 seconds and packaged under various atmospheres in Cryovac bags and stored at 5, 10 and 15$ sp circ$C up to 28 days. Samples were monitored for physical, chemical and microbiological changes throughout the storage period. Optimum control over the growth of L. monocytogenes was achieved using a combination of 100%N$ sb2$ + an Ageless FX oxygen absorbent and dipping in a 0.2% chitosan solution. Based on these studies, a combination of 0.2% chitosan and MAP could be used to extend the shelf life of pork without adversely affecting color, odor and exudate loss while inhibiting the growth of the pathogenic microorganism, L.monocytogenes. (Abstract shortened by UMI.)

La meningite è un'infiammazione delle meningi, le membrane che rivestono il cervello e il midollo spinale. Di solito è causata da batteri o virus, ma può anche essere causato da farmaci o malattie (meningite di eziologia non infettiva). La meningite batterica è rara, ma di solito è grave e può essere pericolosa per la vita se non trattata immediatamente. La meningite virale (detta anche meningite asettica) è relativamente comune e molto meno grave. Rimane spesso non diagnosticata perché i suoi sintomi possono essere simili a quelli della comune influenza. Se tempestivamente diagnosticata, la meningite può essere trattata con successo. Quindi è riconoscere i segni di meningite, e se si sospetta che il bambino ha la malattia, richiedere assistenza medica immediatamente. Molti dei batteri e virus che causano la meningite sono abbastanza comuni e sono in genere associate ad altre patologie di routine. Batteri e virus che infettano la cute, l’apparato urinario, il tratto gastrointestinale o respiratorio possono diffondere per mezzo della circolazione sanguigna verso le meningi e causare il processo infettivo.In alcuni casi di meningite batterica, i batteri si diffondono nelle meningi a cvausa di un grave trauma alla testa o una grave infezione locale, come ad esempio una grave infezione all'orecchio (otite media) o infezione dei seni nasali (sinusite). Diversi tipi di batteri possono causare la meningite batterica. Nei neonati, le cause più comuni sono lo streptococco di gruppo B, Escherichia coli e Listeria monocytogenes. In bambini più grandi, Streptococcus pneumoniae (pneumococco) e Neisseria meningitidis (meningococco) sono più spesso le cause. Un altro batterio, Haemophilus influenzale tipo b (Hib), può anche causare la malattia, ma a causa di vaccinazioni infantili diffuse, questi casi sono diventati più rari. Allo stesso modo, molti virus possono causare la meningite virale, compresi gli enterovirus (come coxsackie, polio ed epatite A) e gli herpesvirus. I sintomi della meningite sono variabili e dipendono sia l'età del bambino / paziente adulto e la causa dell'infezione. Poiché la meningite sintomi simil-influenzali possono essere simili in entrambi i tipi di meningite, soprattutto nelle fasi iniziali, e batteriche possono essere molto gravi, è importante diagnosticare rapidamente l'infezione. La sua importanza deriva non solo dalla sua gravità, l'infezione progredisce rapidamente e può condurre alla morte, ma anche le conseguenze che può causare: danni e invalidità permanenti di vari tipi e livelli. E questo è il motivo per cui il solo sospetto di meningite in un sistema di allarme del paziente e causa una forte implicazione emotiva per tutti i professionisti sanitari coinvolti nel medico di emergenza chiamata solleva la necessità di un approccio metodologico basato sulla velocità, precisione e in particolare sulla scelta di sistemi che sono estremamente sensibili.
Meningitis is an inflammation of the meninges, the membranes that cover the brain and spinal cord. It is usually caused by bacteria or viruses, but it can also be caused by certain medications or illnesses (meningitis of non-infectious etiology). Bacterial meningitis is rare, but is usually serious and can be life-threatening if it's not treated right away. Viral meningitis (also called aseptic meningitis) is relatively common and far less serious. It often remains undiagnosed because its symptoms can be similar to those of the common flu. If promptly diagnosed, meningitis can be treated successfully. So it's important to get routine vaccinations, know the signs of meningitis, and if you suspect that your child has the illness, seek medical care right away. Many of the bacteria and viruses that cause meningitis are fairly common and are typically associated with other routine illnesses. Bacteria and viruses that infect the skin, urinary system, gastrointestinal or respiratory tract can spread by the bloodstream to the meninges through cerebrospinal fluid, the fluid that circulates in and around the spinal cord. In some cases of bacterial meningitis, the bacteria spread to the meninges from a severe head trauma or a severe local infection, such as a serious ear infection (otitis media) or nasal sinus infection (sinusitis). Many different types of bacteria can cause bacterial meningitis. In newborns, the most common causes are Group B streptococcus, Escherichia coli, and Listeria monocytogenes. In older kids, Streptococcus pneumoniae (pneumococcus) and Neisseria meningitidis (meningococcus) are more often the causes. Another bacteria, Haemophilus influenza type b (Hib), can also cause the illness but because of widespread childhood immunization, these cases are now rarer. Similarly, many different viruses can lead to viral meningitis, including enteroviruses (such as coxsackievirus, poliovirus, and hepatitis A) and the herpesvirus. The symptoms of meningitis vary and depend both on the age of the child/adult patient and on the cause of the infection. Because the flu-like symptoms can be similar in both types of meningitis, particularly in the early stages, and bacterial meningitis can be very serious, it's important to quickly diagnose an infection. Its importance stems not only from its gravity, the infection progresses rapidly and can lead to death, but also the consequences that can cause: damage and permanent disabilities of various types and levels. And this is the reason why the mere suspicion of meningitis in a patient cause alarm and strong emotional implications for all health professionals involved in emergency doctor called it raises the need for a methodological approach based on speed, accuracy and especially on the choice of systems that are highly sensitive.

Resonance Raman (RR) spectroscopy complemented by UV-Vis absorption spectroscopy is a very powerful technique to investigate the structure-function relationships of heme proteins, a widely distributed and biological relevant class of proteins which can play different biological functions. Since the protein activity is tightly linked to the structure of the heme active site, my study has been devoted to the investigation of several heme proteins involved in important biological processes, to obtain a comprehensive spectroscopic signature, with the aim to highlight the relationship between the heme pocket architecture and the protein function. The studies were carried out on native proteins and selected site-directed mutants, at both room (298 K) and low (80 K) temperature, at various pH, and in presence of various exogenous ligands, spanning the excitation wavelengths from UV to the visible region.