Дисертації з теми "Live Cell Imaging Biosensors"
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Konishi, Yoshinobu. "Live-cell FRET imaging reveals a role of extracellular signal-regulated kinase activity dynamics in thymocyte motility." Kyoto University, 2019. http://hdl.handle.net/2433/242374.
Повний текст джерелаHung, Yin Pun. "Single Cell Imaging of Metabolism with Fluorescent Biosensors." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10147.
Повний текст джерелаKosmacek, Elizabeth Anne Ianzini Fiorenza Mackey Michael A. "Live cell imaging technology development for cancer research." [Iowa City, Iowa] : University of Iowa, 2009. http://ir.uiowa.edu/etd/388.
Повний текст джерелаChyan, Wen Ph D. Massachusetts Institute of Technology. "Fluorogenic probes for live-cell imaging of biomolecules." Thesis, Massachusetts Institute of Technology, 2018. http://hdl.handle.net/1721.1/118216.
Повний текст джерелаCataloged from PDF version of thesis.
Includes bibliographical references (pages 231-249).
Fluorogenic probes, small-molecule sensors that unmask brilliant fluorescence upon exposure to specific stimuli, are essential tools for chemical biology. Probes that detect enzymatic activity can be used to illuminate the complex dynamics of biological processes at a level of spatiotemporal detail and sensitivity unmatched by other techniques. This dissertation describes the development of new fluorophore chemistries to expand our current fluorogenic probe toolkit and the subsequent application of these probes to study dynamic cell transport processes. Chapter 1. Enzyme-Activated Fluorogenic Probes for Live-Cell and In Vivo Imaging. Chapter 1 reviews recent advances in enzyme-activated fluorogenic probes for biological imaging, organized by enzyme classification. This review surveys recent masking strategies, different modes of enzymatic activation, and the breadth of current and future probe applications. Key challenges, such as probe selectivity and spectroscopic requirements, are described in this chapter along with therapeutic and diagnostic opportunities that can be accessed by surmounting these challenges. Chapter 2. Electronic and Steric Optimization of Fluorogenic Probes for Biomolecular Imaging. In many fluorogenic probes, the intrinsic fluorescence of a small-molecule fluorophore is masked by ester masking groups until entry into a cell, where endogenous esterases catalyze the hydrolysis of esters, generating fluorescence. The susceptibility of masking groups to spontaneous hydrolysis is a major limitation of these probes. Previous attempts to address this problem have incorporated auto-immolative linkers at the cost of atom economy and synthetic adversity. In this chapter, I report on a linker-free strategy that employs adventitious electronic and steric interactions in easy-to-synthesize probes. I find that halogen-carbonyl n-->[pi]* interactions and acyl group size are optimized in 2',7'-dichlorofluorescein diisobutyrate. This probe is relatively stable to spontaneous hydrolysis but is a highly reactive substrate for esterases both in vitro and in cellulo, yielding a bright, photostable fluorogenic probe with utility in biomolecular imaging. Chapter 3. Cellular Uptake of Large Monofunctionalized Dextrans. Dextrans are a versatile class of polysaccharides with applications that span medicine, cell biology, food science, and consumer goods. In Chapter 3, I apply the electronically stabilized probe described in Chapter 2 to study the cellular uptake of a new type of large monofunctionalized dextran that exhibits unusual properties: efficient cytosolic and nuclear uptake. This dextran permeates various human cell types without the use of transfection agents, electroporation, or membrane perturbation. Cellular uptake occurs primarily through active transport via receptor-mediated processes. These monofunctionalized dextrans could serve as intracellular delivery platforms for drugs or other cargos. Chapter 4. Paired Nitroreductase-Probe System to Quantify the Cytosolic Delivery of Biomolecules. Cytosolic delivery of large biomolecules is a significant barrier to therapeutic applications of CRISPR, RNAi, and biologics such as proteins with anticancer properties. In Chapter 4, I describe a new paired enzyme-probe system to quantify cytosolic delivery of biomolecules-a valuable resource for elucidating mechanistic details and improving delivery of therapeutics. I designed and optimized a nitroreductase fusion protein that embeds in the cytosolic face of outer mitochondrial membranes, providing several key improvements over unanchored reporter enzymes. In parallel, I prepared and assessed a panel of nitroreductase-activated probes for favorable spectroscopic and enzymatic activation properties. Together, the nitroreductase fusion protein and fluorogenic probes provide a rapid, generalizable tool that is well-poised to quantify cytosolic delivery of biomolecules. Chapter 5. Future Directions. This chapter outlines several future directions for expanding the scope of fluorogenic probes and developing new biological applications. Additionally, Chapter 5 is followed by an appendix describing a tunable rhodol fluorophore scaffold for improved spectroscopic properties and versatility. Overall, the work described in this thesis illustrates the power of enzyme-activated fluorogenic probes to provide fresh insight into dynamic biological processes, with direct implications for improved therapeutic delivery.
by Wen Chyan.
Ph. D. in Biological Chemistry
Büchele, Benjamin. "Live Cell Imaging des Hepatitis C Virus Replikationskomplexes." [S.l. : s.n.], 2004. http://nbn-resolving.de/urn:nbn:de:bsz:25-opus-59102.
Повний текст джерелаKosmacek, Elizabeth Anne. "Live cell imaging technology development for cancer research." Diss., University of Iowa, 2009. https://ir.uiowa.edu/etd/388.
Повний текст джерелаCaporale, Chiara. "Luminescent Iridium Tetrazolato Markers for Live Cell Imaging." Thesis, Curtin University, 2018. http://hdl.handle.net/20.500.11937/70386.
Повний текст джерелаDanylchuk, Dmytro. "Environment-sensitive targeted fluorescent probes for live-cell imaging." Thesis, Strasbourg, 2021. http://www.theses.fr/2021STRAF012.
Повний текст джерелаSpecific targeting, imaging and probing of cell plasma membranes and intracellular organelles can be addressed by rationally designed polarity-sensitive fluorescent probes. Here, a new efficient plasma membrane-targeting moiety was developed and tested in five cyanine dyes, showing excellent performance in cellular and in vivo microscopy. Next, the targeting moiety was grafted to a solvatochromic dye Prodan, yielding a plasma membrane probe with high lipid order sensitivity. Modifying a Nile Red using the moieties with varied alkyl chain lengths resulted in two solvatochromic plasma membrane probes: NR12A with high affinity to membranes for conventional microscopy, and NR4A, a low-affinity probe for PAINT super-resolution microscopy. Tethering Nile Red with organelle-targeted groups yielded an array of probes, able to sense polarity and lipid order in organelle membranes. The synthesized probes will find applications in bioimaging, cell biology, biophysics or mechanobiology
Sörman, Paulsson Elsa. "Evaluation of In-Silico Labeling for Live Cell Imaging." Thesis, Umeå universitet, Institutionen för fysik, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-180590.
Повний текст джерелаHan, Hongqing. "Towards accurate and efficient live cell imaging data analysis." Doctoral thesis, Humboldt-Universität zu Berlin, 2021. http://dx.doi.org/10.18452/22324.
Повний текст джерелаLive cell imaging based on time-lapse microscopy has been used to study dynamic cellular behaviors, such as cell cycle, cell signaling and transcription. Extracting cell lineage trees out of a time-lapse video requires cell segmentation and cell tracking. For long term live cell imaging, data analysis errors are particularly fatal. Even an extremely low error rate could potentially be amplified by the large number of sampled time points and render the entire video useless. In this work, we adopt a straightforward but practical design that combines the merits of manual and automatic approaches. We present a live cell imaging data analysis tool `eDetect', which uses post-editing to complement automatic segmentation and tracking. What makes this work special is that eDetect employs multiple interactive data visualization modules to guide and assist users, making the error detection and correction procedure rational and efficient. Specifically, two scatter plots and a heat map are used to interactively visualize single cells' visual features. The scatter plots position similar results in close vicinity, making it easy to spot and correct a large group of similar errors with a few mouse clicks, minimizing repetitive human interventions. The heat map is aimed at exposing all overlooked errors and helping users progressively approach perfect accuracy in cell lineage reconstruction. Quantitative evaluation proves that eDetect is able to largely improve accuracy within an acceptable time frame, and its performance surpasses the winners of most tasks in the `Cell Tracking Challenge', as measured by biologically relevant metrics.
Flaccavento, Giselle. "Imaging tools for live cell micro-irradiation survival studies." Thesis, University of Oxford, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.589627.
Повний текст джерелаSaurabh, Saumya. "Ultra-Photostable Genetically Targeted Fluoromodules for Live Cell Imaging." Research Showcase @ CMU, 2014. http://repository.cmu.edu/dissertations/1020.
Повний текст джерелаKhorshidi, Mohammad Ali. "Live Single Cell Imaging and Analysis Using Microfluidic Devices." Doctoral thesis, KTH, Proteomik och nanobioteknologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-129278.
Повний текст джерелаQC 20130927
Miura, Haruko. "Live-Cell Imaging of Stress Signaling Dynamics in a Cell Fate Decision." Kyoto University, 2019. http://hdl.handle.net/2433/236635.
Повний текст джерелаBabic, Julien. "New microfluidic systems for controlling the cell microenvironment during live-cell imaging." Thesis, Rennes 1, 2017. http://www.theses.fr/2017REN1B047/document.
Повний текст джерелаMonitoring in real-time the response of cells and model organisms to the changes in their environment or to modulations of their biological functions has become essential in life sciences. One of the main technical challenges for biologists is the precise and dynamic control of various environmental parameters while doing high-resolution microscopy. My thesis consists of building a robust and versatile system, dedicated to live-cell imaging that will be compatible with adherent and non adherent models, that could provide a precise and simultaneous control of 1) the temperature, 2) the media exchanges and 3) the drug concentration while doing photonic microscopy. My approach is to use microfluidics, which is the best candidate in order to achieve this system and provides all the necessary controls of micro-scaled volumes for culturing, maintaining or analyzing cells. It produces miniaturized systems used as tools for biological experiments, in which channels of a micro-scaled dimension are used for the fluid circulation. The laminar flow in these chips allows fast molecule diffusion as well as fast temperature diffusion. Because of the high surface to volume ratio, the consumption of reagents is reduced, and media switches can be fast. This system will represent a major technical and beneficial step and will open new possibilities of research in biology
Sauer, Anna Magdalena. "Live-cell imaging of drug delivery by mesoporous silica nanoparticles." Diss., lmu, 2011. http://nbn-resolving.de/urn:nbn:de:bvb:19-138222.
Повний текст джерелаRosa, Stefanie. "Chromatin dynamics in Arbidopsis development: a live cell imaging approach." Doctoral thesis, Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica, 2011. http://hdl.handle.net/10362/6847.
Повний текст джерелаThe proper development of multicellular organisms demands the distinct specification of a variety of specialized cell types. While this is one of the oldest statements of developmental genetics, how different patterns of gene expression are established in genetically identical cells and maintained during somatic cell divisions is still an active topic of research. Chromatin structure is now recognized to regulate gene activity playing a crucial role in cell differentiation and development. Chromatin is not simply a packaging tool but a dynamic entity that reflects the regulatory cues necessary to program appropriate cellular pathways. There are several ways by which chromatin structure can be remodelled. These mechanisms include DNA-methylation, post-translational modifications of histone proteins, histone variants and, nuclear localization. While the dynamic nature of chromatin structure has been previously described its biological function and repercussions on development are only now beginning to be revealed. In this work we used in vivo microscopy techniques to assess how different aspects of chromatin organization play a role on various aspects of development.(...)
A bolsa de doutoramento com a referência SFRH/BD/23202/2005 foi atribuída pela Fundação para a Ciência e Tecnologia (FCT), no âmbito do Quadro Comunitário de Apoio, comparticipado pelo Fundo Social Europeu (FSE).
Forsgren, Edvin. "Deep Learning to Enhance Fluorescent Signals in Live Cell Imaging." Thesis, Umeå universitet, Institutionen för fysik, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-175328.
Повний текст джерелаYao, Zhizhong. "Using Live Cell Imaging to Probe Biogenesis of the Gram-Negative Cell Envelope." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10230.
Повний текст джерелаChemistry and Chemical Biology
Smith, David. "Process monitoring and control using live cell imaging for the manufacturing of cell therapies." Thesis, Loughborough University, 2014. https://dspace.lboro.ac.uk/2134/16063.
Повний текст джерелаAuciello, Giulio. "Analysis of FGF receptor signalling and trafficking by live-cell imaging." Thesis, University of Birmingham, 2013. http://etheses.bham.ac.uk//id/eprint/4650/.
Повний текст джерелаHan, Hongqing [Verfasser]. "Towards accurate and efficient live cell imaging data analysis / Hongqing Han." Berlin : Humboldt-Universität zu Berlin, 2021. http://d-nb.info/1226153445/34.
Повний текст джерелаHailey, Dale W. "Live cell imaging to study the assembly and fate of autophagosomes." College Park, Md.: University of Maryland, 2008. http://hdl.handle.net/1903/8796.
Повний текст джерелаThesis research directed by: Dept. of Cell Biology and Molecular Genetics. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.
Dodgson, Lauren. "Dissecting the molecular mechanisms of Drosophila border cell migration using time-lapse live cell imaging." Thesis, University of Liverpool, 2013. http://livrepository.liverpool.ac.uk/16293/.
Повний текст джерелаMickler, Frauke Martina. "Live-cell imaging elucidates cellular interactions of gene nanocarriers for cancer therapy." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-165829.
Повний текст джерелаLinnik, Volha. "Functional analysis of a plant virus replication 'factory' using live cell imaging." Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/4639.
Повний текст джерелаZhai, Weichao. "Microfluidics and live imaging advances : applications in host/pathogen, immunity and stem cell single cell phenotyping." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/277189.
Повний текст джерелаMoreira, Severina. "Live imaging and genetic studies of inflammatory cell migration in Drosophila melanogaster embryos." Thesis, University of Bristol, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.508069.
Повний текст джерелаMizusawa, Keigo. "Development of Fluorescent Turn-on Self-assembled Nanoprobes for Imaging Specific Proteins under Live Cell Conditions." 京都大学 (Kyoto University), 2013. http://hdl.handle.net/2433/174966.
Повний текст джерелаYatsuzuka, Kenji. "Live-cell imaging of multiple endogenous mRNAs permits the direct observation of RNA granule dynamics." Kyoto University, 2019. http://hdl.handle.net/2433/242400.
Повний текст джерелаKnopp, Marcus. "Analysis of spine plasticity in CA1 hippocampal pyramidal neurons employing live cell nanoscopic imaging." Diss., Ludwig-Maximilians-Universität München, 2014. http://nbn-resolving.de/urn:nbn:de:bvb:19-173975.
Повний текст джерелаThe majority of excitatory synapses in the cortex of mammalian brains is situated on dendritic spines, small protrusions, heterogeneous in size and shape. The induction of activity-dependent long-term synaptic plasticity has been associated with changes in the ultrastructure of spines, particularly in size, head shape and neck width. Since the dimensions of dendritic spines are at the border of the diffraction-limited resolving power of conventional light microscopes, until recently, electron microscopy on fixed tissue constituted the primary method for investigations on spine morphology. I have employed live cell stimulated emission depletion imaging to analyse spine motility and structural transitions in response to n-methyl-d-aspartate receptor dependent long-term potentiation over time at super-resolution in Cornu Ammonis area 1 pyramidal neurons of the hippocampus. Local induction of long-term potentiation via ultraviolet photolysis of caged glutamate facilitated a strong transient increase in the proportion of spines with curved heads and a subtle persistent growth in the amount of mushroom spines over a time course of 50 minutes. My findings reinforce previous investigations on the relation of synaptic potentiation and spine motility, and are in good agreement with the current knowledge of the molecular mechanisms underlying long-term plasticity.
Zhang, Yun. "Real time imaging of live cell ATP leaking or release events by chemiluminescence microscopy." [Ames, Iowa : Iowa State University], 2008.
Знайти повний текст джерелаBhat, Anayat [Verfasser]. "Live Cell Fluorescence Imaging of Nucleotide Dynamics : ATP Hydrolysis and DNA Damage Response / Anayat Bhat." Konstanz : KOPS Universität Konstanz, 2021. http://d-nb.info/1229351094/34.
Повний текст джерелаAltenbach, Kirsten. "Development and analysis of recombinant fluorescent probes for use in live cell imaging of filamentous fungi." Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/4739.
Повний текст джерелаPrusicki, Maria Ada [Verfasser], and Arp [Akademischer Betreuer] Schnittger. "Live cell imaging of meiosis in anthers of Arabidopsis thaliana / Maria Ada Prusicki ; Betreuer: Arp Schnittger." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2019. http://d-nb.info/1192442814/34.
Повний текст джерелаMaruno, Takahisa. "Visualization of stem cell activity in pancreatic cancer expansion by direct lineage tracing with live imaging." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/265166.
Повний текст джерела新制・論文博士
博士(医学)
乙第13427号
論医博第2231号
新制||医||1053(附属図書館)
京都大学大学院医学研究科医学専攻
(主査)教授 松田 道行, 教授 渡邊 直樹, 教授 川口 義弥
学位規則第4条第2項該当
Doctor of Medical Science
Kyoto University
DFAM
Prusicki, Maria Ada Verfasser], and Arp [Akademischer Betreuer] [Schnittger. "Live cell imaging of meiosis in anthers of Arabidopsis thaliana / Maria Ada Prusicki ; Betreuer: Arp Schnittger." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2019. http://nbn-resolving.de/urn:nbn:de:gbv:18-98605.
Повний текст джерелаMonypenny, James Edward. "Development of quantitative live cell imaging techniques and their applications in the study of inter-cellular communication and Sarcoma cell motility." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.406165.
Повний текст джерелаBornheimer, Scott Joseph. "Spatial and temporal regulation of G-protein signaling elucidated by computational modeling and live cell FRET imaging." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2008. http://wwwlib.umi.com/cr/ucsd/fullcit?p3308008.
Повний текст джерелаTitle from first page of PDF file (viewed June 12, 2008). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references.
White, Katharine Alice. "Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging." Thesis, Massachusetts Institute of Technology, 2012. http://hdl.handle.net/1721.1/78438.
Повний текст джерелаCataloged from PDF version of thesis.
Includes bibliographical references.
Chemical fluorophores have superior photophysical properties to fluorescent proteins and are much smaller. However, in order to use these probes for live-cell protein imaging, highly specific labeling methods are required. Here, we will describe three efforts to re-engineer the E. coli enzyme, lipoic acid ligase (LplA), to catalyze the ligation of small-molecule probes onto recombinant proteins. We call this collection of methods the PRIME (PRobe Incorporation Mediated by Enzymes) methodologies. First, we describe the structure-guided mutagenesis of LplA and the identification of an LplA variant that can ligate a blue coumarin fluorophore onto a 13-amino acid LplA acceptor peptide (LAP2). This "coumarin ligase" can be used to image cellular proteins with high specificity, sensitivity, and minimal perturbation of the biology of the protein of interest. We also demonstrate how subpopulations of a protein of interest can be labeled using genetically targeted coumarin ligase. Second, we describe our attempts to use yeast display evolution and fluorescence activated cell sorting (FACS) to evolve a truncated LplA enzyme. The original truncated enzyme had severely decreased activity for LplA's natural substrate, lipoic acid. We created a 107 library of LplA mutants and, after four rounds of selection, produced a truncated LplA mutant with lipoylation activity equivalent to full-length LplA. We next sought to evolve activity for an unnatural small molecule probe, but found that this strategy was limited by both increased hydrophobic probe sticking when using the truncated enzyme and some enzyme-dependent nonspecificity. Finally, from a library of 107 LplA mutants, we evolved a full-length LplA capable of ligating an unnatural picolyl azide (pAz) substrate. We demonstrated improved activity of the "pAz ligase" in the secretory pathway and cell surface, two regions where coumarin ligase is inactive. This enzyme can also be used to image cell surface protein-protein interactions as well as label proteins as they are trafficked through the endoplasmic reticulum. These probe ligases will be useful tools for cell biologists interested in studying protein function or protein-protein interactions in the context of living cells.
by Katharine Alice White.
Ph.D.
Gustafsson, Linnéa. "Internalisation of antigen-adjuvant conjugate in human dendritic cells : An assay development for using live cell imaging." Thesis, Uppsala universitet, Institutionen för farmaceutisk biovetenskap, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-434224.
Повний текст джерелаPanday, Namuna. "Scanning Ion Conductance Microscopy for Single Cell Imaging and Analysis." FIU Digital Commons, 2017. http://digitalcommons.fiu.edu/etd/3477.
Повний текст джерелаAli, Rizwan. "Live Cell Imaging of Intracellular Uptake of Contaminant Molecules (B[a]P) and its Effects on Different Cellular Compartments." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2012. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-91967.
Повний текст джерелаMickler, Frauke Martina [Verfasser], and CHRISTOPH [Akademischer Betreuer] BRAEUCHLE. "Live-cell imaging elucidates cellular interactions of gene nanocarriers for cancer therapy / Frauke Martina Mickler. Betreuer: Christoph Bräuchle." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2013. http://d-nb.info/1047300516/34.
Повний текст джерелаDittrich, Florian [Verfasser], and Stefan [Akademischer Betreuer] Landgraeber. "Untersuchungen zum abriebpartikelinduzierten Zelltod mittels "live-cell Imaging" im Kontext der aseptischen Endoprothesenlockerung / Florian Dittrich ; Betreuer: Stefan Landgraeber." Duisburg, 2018. http://d-nb.info/1155097262/34.
Повний текст джерелаJensen, Rebecca Leah. "Live Cell Imaging to Investigate Bone Marrow Stromal Cell Adhesion and Migration on Titanium Surfaces: A Micro-Incubator in vitro Model." Cleveland State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=csu1391128419.
Повний текст джерелаDursun, Ezgi [Verfasser], Anne [Akademischer Betreuer] Krug, Thomas [Gutachter] Korn, and Markus [Gutachter] Gerhard. "Cell fate decisions of common dendritic cell progenitors characterized by continuous live cell imaging at the single cell level / Ezgi Dursun ; Gutachter: Thomas Korn, Markus Gerhard ; Betreuer: Anne Krug." München : Universitätsbibliothek der TU München, 2015. http://d-nb.info/1114393983/34.
Повний текст джерелаWen, Mary Mei. "New strategies for tagging quantum dots for dynamic cellular imaging." Diss., Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/52150.
Повний текст джерелаHelfrich, Stefan Verfasser], Wolfgang [Akademischer Betreuer] Wiechert, and Björn [Akademischer Betreuer] [Usadel. "High-throughput live-cell imaging for investigations of cellular heterogeneity in Corynebacterium glutamicum / Stefan Helfrich ; Wolfgang Wiechert, Björn Usadel." Aachen : Universitätsbibliothek der RWTH Aachen, 2016. http://d-nb.info/1129875989/34.
Повний текст джерелаBoni, Andrea [Verfasser], and Jan [Akademischer Betreuer] Ellenberg. "Inner nuclear membrane protein targeting studied by quantitative live cell imaging and RNAi screening / Andrea Boni ; Betreuer: Jan Ellenberg." Heidelberg : Universitätsbibliothek Heidelberg, 2016. http://d-nb.info/1180608046/34.
Повний текст джерела