Дисертації з теми "Lipid Probes"
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Garton, Natalie Jane. "Investigation of mycobacterial lipid domains by use of fluorescent lipid probes." Thesis, University of Newcastle Upon Tyne, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244396.
Повний текст джерелаGäbler, Anne [Verfasser]. "Alkyne lipid probes and azide detection reagents for in vitro enzymatic assays and highly sensitive lipid imaging / Anne Gäbler." Bonn : Universitäts- und Landesbibliothek Bonn, 2015. http://d-nb.info/1113688173/34.
Повний текст джерелаXiaoqian, Chen. "Liposome and drug-targeted molecular probes for detecting lipid droplets and tracking cancer cells." Магістерська робота, Kyiv National University of Technology and Design, 2021. https://er.knutd.edu.ua/handle/123456789/19264.
Повний текст джерелаЛіпідні краплі (LD) вважаються органелами з надзвичайно низьким вмістом води та високою в’язкістю. Пов’язані з такими захворюваннями, як цукровий діабет, рак, тобто, коли хвороба є аномальною, у клітинах з’являться ліпідні краплі, тому ми розробили чотири типи ліпідних крапель. Розроблено просту п-нітрофенбутилетилову сполуку, що поглинає кумарин, як потенційний новий органічний біокаталізатор для груп візуалізації. Внутрішній проекційний спектр зміщується в видимій області світла. Крім того, сполуку виготовляють на основі донорського матеріалу. Камера Стокса (100 нм, більш ніж хороший LD, низька біологічна токсичність і низька біологічна токсичність і введення). Синтезовано два нових зонди, LDP-1 і LDP-2, які показали роздільну здатність 4758 см-1 і 3986 см-1 відповідно. Крім того, біологічні зонди LDP-1 і LDP-2 демонструють низьку біологічну токсичність і хорошу специфічність. Ці два зонди також підходять для моніторингу життєвого циклу вивільнення клітинної LD в HeLa. Розроблено новий тип люмінесцентного хімічного датчика, який може ефективно позначати внутрішню частину клітини.
Sachl, Radek. "Localisation of Fluorescent Probes and the estimation of Lipid Nanodomain sizes by modern fluorescence techniques." Doctoral thesis, Umeå universitet, Kemiska institutionen, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-52619.
Повний текст джерелаDisertace je rozdělena do dvou hlavníchčástí. Prvníčást se zabývá lokalizací značek v lipidových/polymerních dvojvrstvách a v GM1micelách. V práci prezentujeme nový přístup založený na přenosu/migraci elektronické energie (FRET/DDEM), jež umožňuje efektivně určovat vertikální pozici fluorescenčních molekul uvnitř lipidové dvojvrstvy. Tato metoda byla použita k lokalizaci nově syntetizovaných lipidových značek značených na konci sn-2 acylového řetězce s různou délkou v DOPC dvojvrstvách. Analytické modely popisující FRET existují pouze pro limitovaný počet základních geometrií. Kombinace FRETu s Monte Carlo simulacemi nicméně umožňuje lokalizaci značek v bicelách a v dvojvrstvách obsahujících póry, tj. v lipidových systémech s proměnlivým zakřivením a v nehomogenních lipidových útvarech. Tento přístup umožnil např. zjistit, zda kuželovitětvarované značky mají zvýšenou afinitu k vysoce zakřiveným oblastem dvojvrstvy, což by umožnilo preferenční značení pórů. Lokalizovány byly rovněž tři deriváty 2-pyridonů(potencionálních léčiv) v GM1micelách za použití jednoduchého modelu zohledňujícího FRET mezi donory a akceptory nacházejícími se v micelách. Lokalizace léčiv v nanočásticích ovlivňuje kinetiku uvolňování (release kinetics) a množství látky solubilizované v micelách (loading efficiency). Druhá část se především zabývá určováním velikostí lipidových nanodomén pomocí FRETu, který stále zůstává nejvíce výkonnou metodou v této oblasti. Zkoumány byly limitace FRETu v určování lipidových nanodomén. Ukázalo se, že tato omezení jsou především způsobena nízkou afinitou značek buď k Lonebo k Ldfázi. V navazující studii jsme poskytnuli detailní dynamickou a strukturní studii formace nanodomén indukované crosslinkerem. Objevili jsme dva typy domén: a) domény, jejichž velikost se zvětšuje s rostoucím množstvím přidaného cholera toxinu (CTxB) a k nimž se CTxB váže pevně a b) domény vzniklé v membránách se zvýšeným množstvím sfingomyelinu (ve srovnání s a)), jejichž velikost se nemění během titrace dodatečným CTxB a k nimž se CTxB váže méně pevně.
This thesis has been elaborated within the framework of the Agreement on JointSupervision (co-tutelle) of an International Doctoral Degree Programmebetween Charles University in Prague, Czech Republic and the Department of Chemistry at Umeå University, Sweden.
Carter, Ramirez Daniel Marcelo. "Fluorescent and Photocaged Lipids to Probe the Ceramide-mediated Reorganization of Biological Membranes." Thèse, Université d'Ottawa / University of Ottawa, 2013. http://hdl.handle.net/10393/23713.
Повний текст джерелаDanylchuk, Dmytro. "Environment-sensitive targeted fluorescent probes for live-cell imaging." Thesis, Strasbourg, 2021. http://www.theses.fr/2021STRAF012.
Повний текст джерелаSpecific targeting, imaging and probing of cell plasma membranes and intracellular organelles can be addressed by rationally designed polarity-sensitive fluorescent probes. Here, a new efficient plasma membrane-targeting moiety was developed and tested in five cyanine dyes, showing excellent performance in cellular and in vivo microscopy. Next, the targeting moiety was grafted to a solvatochromic dye Prodan, yielding a plasma membrane probe with high lipid order sensitivity. Modifying a Nile Red using the moieties with varied alkyl chain lengths resulted in two solvatochromic plasma membrane probes: NR12A with high affinity to membranes for conventional microscopy, and NR4A, a low-affinity probe for PAINT super-resolution microscopy. Tethering Nile Red with organelle-targeted groups yielded an array of probes, able to sense polarity and lipid order in organelle membranes. The synthesized probes will find applications in bioimaging, cell biology, biophysics or mechanobiology
Kreder, Rémy. "Sondes moléculaires multifonctionnelles pour l'imagerie de fluorecence de membranes cellulaires." Thesis, Strasbourg, 2015. http://www.theses.fr/2015STRAJ006/document.
Повний текст джерелаBased on rational molecular design, we design new membrane probes that enable fluorescence imaging of cell plasma membrane organization. In this work, we first synthesized a toolkit, based on solvatochromic Nile Red dye and Black Hole Quencher-2, that can stain specifically ordered and disordered lipid domains (rafts) and identify them by the emission color. Cellular studies with these probes suggested that the plasma membrane is composed of two distinct phases. Then,with the idea to make Nile Red-based probes compatible with serum medium and fixable by formaldehyde/glutaraldehyde, we modified previously developed probe NR12S with PEG and aminogroups, respectively. Surprisingly, the PEGylated probe is quickly internalized inside the cell and the amino-derivative aggregates with the fixing agent. On the other hand, based on Nile Red we designed probes capable to detect a given receptor and visualize its lipid environment. Initially, we obtained probes that can turn-on fluorescence on binding to the oxytocin GPCR receptor. Then, we conjugated NR12S through a PEG(12) spacer to the ligand of intergrin, RGD. The first data show that the molecule can bind to the membrane and detect the lipid order, though cellular studies have to be completed. We also worked on fluorogenic (turn-on) membrane probes for multi-color imaging. Based on blue 3-methoxychromone dyes, we obtained probes that are brighter and more photostable than the originally developed probe based on 3-hydroxychromone (F2N12S). Due to large Stocks shift, they enabled cell membrane imaging with minimal auto-fluorescence in the blue spectral region, compatible with common green and red probes. At the end, based on squaraine fluorophore, we developed three new probes operating in the far red region, which is also very interesting for in vitro and in vivo imaging. These dyes show a parallel orientation with the lipid membrane, while the cellular experiments point out that only the probe with two anchor groups is able to stain stably the plasma membrane. The probes developed here are expected to be used for lipid rafts research as well as for super-resolution and multi-color imaging of living cells
Burdíková, Jana. "Fosfolipidy jako základ biodegradabilních nosičových systémů." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2013. http://www.nusl.cz/ntk/nusl-216959.
Повний текст джерелаZhao, Yue. "Synthetic probes for bacterial lipids and dimerizing proteins." Thesis, Boston College, 2015. http://hdl.handle.net/2345/bc-ir:104623.
Повний текст джерелаThis thesis includes two projects: “Bacteria-selective borono-peptides” and “A split ligand for lanthanide binding: facile evaluation of dimerizing proteins”. In both projects, de novo designed molecules were synthesized, optimized and incorporated into peptides. These synthetic molecular tools allow selective targeting of bacterial cell membranes and analyzing the dynamic associations of membrane-embedded proteins. 1. Bacteria-selective borono-peptides As the antibiotic resistance continues to grow, bacterial infection becomes one of the major threats to global public health. Currently, almost all the bacteria targeting strategies employ non-covalent driving forces, including charge-charge interactions, hydrophobic interactions and the formation of hydrogen bonds, to achieve bacterial selectivity. Towards novel bacteria targeting molecules, we have recruited reversible covalent chemistry in the development of bacteria-selective peptides. Targeting the diol-rich environment of a bacterial surface, we have designed and synthesized several unnatural amino acids that contain boronic acid moieties. Taking advantage of the boronic acid-diol reaction and multivalency effect, our borono-peptides are found to selectively recognize bacteria over mammalian cells. The sensitivity of the binding event to carbohydrate competitors gives a safe and facile approach to regulate molecular association with bacterial cells. This design may find applications in the fields of bacterial detection, imaging and antimicrobial drug delivery. 2. A split ligand for lanthanide binding: facile evaluation of dimerizing proteins Protein dimerization is a ubiquitous phenomenon in biology and plays a critical role in transcription regulations and various signaling processes. Methods that allow facile detection and quantification of protein dimers are highly desirable for evaluating protein dimerization in physiology and disease. Meanwhile, luminescence of lanthanides is attractive for biological applications due to its long lifetime and sharp emission profiles. We have developed a split lanthanide binding ligand that allows facile evaluation of dimerizing proteins. The fast lanthanide–ligand (dis)association allows us to monitor the dynamic behavior of dimerizing proteins. We have demonstrated the successful application of our assay on both soluble and transmembrane proteins in complex biological milieu. The split lanthanide ligand is cysteine reactive, and therefore should be readily applicable to a variety of proteins of interest
Thesis (PhD) — Boston College, 2015
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Chemistry
Kelly, Michael A. "Developing Peptide Probes for Membrane Lipids via Phage Display:." Thesis, Boston College, 2020. http://hdl.handle.net/2345/bc-ir:108919.
Повний текст джерелаLipid reporters are key signaling molecules in a number of biological processes ranging from apoptosis in mammalian cells to novel resistance mechanisms in pathogenic bacteria. Developing probes to target these lipids is a worthy endeavor, especially when better reporters could mean lives saved. This is particularly true considering new antibiotic resistant pathogens emerge every year with evolving lipid compositions. To combat these pathogens and prevent a potential global pandemic, it is imperative to continue the development of novel and innovative probes/drugs to meet this daunting challenge. To fulfill this demand, we must continue to establish new strategies, enhance current technologies and advance scientific understanding. Only by pushing the boundaries of what is currently possible will we remain one step ahead of these diseases. Diseases like mcr-1 positive bacteria, first documented in 2016, remain largely uncontested. Herein, we seek to expand the available probes specific to key lipid reporters for phosphatidylserine, lysyl-phosphatidylglycerol, and phosphoethanolamine lipid A. Cyclic phage libraries were first utilized to target phosphatidylserine, ultimately producing weak binders. Refining our phage display libraries to include reversible covalent warheads allowed for the identification of more potent lipid reporters. In doing so, we have created the tools necessary to interrogate the unique resistance mechanisms expressed by these drug-resistant pathogens. A strong correlation was observed between peptides binding mcr-1 positive strains, LPS modification on the surface of these bacteria, and level of colistin resistance. To our knowledge, these peptides are the only probes capable of demonstrating this correlation. We surmise that the methods discussed here will pave the way for better diagnostic tools for these resistant pathogens. A recurring method of resistance among gram-positive and gram-negative bacteria has been to decorate their surface with positive amines to repel cationic antimicrobial peptides. As such, our current APBA library and the libraries in development in the Gao lab would be ideally suited to target these and other undiscovered resistance mechanisms
Thesis (PhD) — Boston College, 2020
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Chemistry
Chiantia, Salvatore. "Protein-lipid interactions in raft-exhibiting membranes probed by combined AFM and FCS." Doctoral thesis, [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1216391330086-30964.
Повний текст джерелаChiantia, Salvatore. "Protein-lipid interactions in raft-exhibiting membranes probed by combined AFM and FCS." Doctoral thesis, Technische Universität Dresden, 2007. https://tud.qucosa.de/id/qucosa%3A23617.
Повний текст джерелаCao, Huachuan. "Probe Oxidative Damage in DNA Charge Transfer Process." Diss., Georgia Institute of Technology, 2005. http://hdl.handle.net/1853/6983.
Повний текст джерелаVaccaro, Luciana. "Local probe microscopy on lipid membranes : near field optical imaging and shear force studies /." [S.l.] : [s.n.], 2000. http://library.epfl.ch/theses/?nr=2266.
Повний текст джерелаWorthman, Lynn-Ann D. "Surfactant protein A (SP-A) affects pulmonary surfactant morphology and biophysical properties." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0014/MQ34241.pdf.
Повний текст джерелаSchütte, Ole Mathis. "Structure and dynamics of artificial lipid membranes containing the glycosphingolipid Gb3." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://hdl.handle.net/11858/00-1735-0000-0023-960D-7.
Повний текст джерелаTroup, Gregory Marshall Wrenn Steven Parker Dr. "Fluorescence investigation of laterally phase-separated cholesterol rich domains in model lipid membranes using the membrane probe 1-myristoyl-2-[12-[(5-dimethylamino-1-naphthalenesulfonyl)amino]dodecanoyl]-sn-Glycero-3-phosphocholine (A) /." Philadelphia, Pa. : Drexel University, 2004. http://dspace.library.drexel.edu/handle/1860/345.
Повний текст джерелаHahnefeld, Lisa Katharina [Verfasser], Nerea Ferreirós [Gutachter] Bouzas, and Achim [Gutachter] Schmidtko. "Verwendung hochauflösender Massenspektrometrie zur Suche neuer Lipid-Biomarker in biologischen Proben / Lisa Katharina Hahnefeld ; Gutachter: Nerea Ferreirós Bouzas, Achim Schmidtko." Frankfurt am Main : Universitätsbibliothek Johann Christian Senckenberg, 2020. http://d-nb.info/1221669249/34.
Повний текст джерелаTaylor, Ryan M. "Bioinformatic Solutions to Complex Problems in Mass Spectrometry Based Analysis of Biomolecules." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/5585.
Повний текст джерелаLi, Hao. "In vitro Studies of Genodermatoses Affecting Cytoskeletal Integrity and Lipid Processing in Human Epidermis : Pathogenic Mechanisms and Effects of Retinoid Therapy." Doctoral thesis, Uppsala universitet, Institutionen för medicinska vetenskaper, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-172863.
Повний текст джерелаBraunger, Julia. "Ezrin activation in vitro: Investigation of ezrin's conformation and the interaction between ezrin and F-actin." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2013. http://hdl.handle.net/11858/00-1735-0000-0022-609D-5.
Повний текст джерелаBarucha-Kraszewska, Justyna. "Experimental and stimulation analyses of fluorescent solvent relaxation process in biomembranes : Inflence of ions and molecular interpretation of the dye dynamics." Thesis, Besançon, 2012. http://www.theses.fr/2012BESA3010/document.
Повний текст джерелаMany biologically important processes and phcnomena in lipid membranes are still not fully understood. The presence of ions and water molœules has a significant influence on the structural and dynamical properties of lipid bilayers. Fluorescent techniques are versatile tools for studying the lipid membranes, because the fluorescence emission is strongly sensitive to dye environment. We have conducted fluorescent solvent relaxation (SR) experiments to explore the hydration and mobility properties in lipid membranes in the presence of different chaotropic ions. We have also carried out Quantum Mechanical (QM) calculations and Molecular Dynamics (MD) simulations for supporting the SR experiments. SR experiments show that small cation (Na+) is attracted to the membrane and increases rigidity ofbilayer, while larger cations (NH/, Cs+) should not. Large anions (CI04·, SCN') adsorl, at the membrane interface more easily than smaller ones (Cl') and significantly change tl!e mobility and hydration of the headgroup region oflipid bilayer. SR study ofhydrophobic part of the membrane show that SR processes are complex there and reflect botl!: faster, intramolecular (torsional relaxation or fonnation of charge transfer state) and slower, intermolecular (SR) relaxation processes. QM calculatiom were used to create force-field for three fluorescent dyes (Prodan, Laurdan and C-laurdan). MD simulations allow detennining position of the dye in the lipid membrane in the ground state and after excitation and reproduce correctly SR timescale- ps in water and ns in the membrane. MD simulations extend the capabilities of SR method and allow observing the behaviour of individual molecules
Rajpal, Ashdeep Kaur. "Design and Synthesis of Metabolically Stabilized Lipid Probes for the Investigation of Protein–Lipid Binding Interactions." 2011. http://trace.tennessee.edu/utk_gradthes/905.
Повний текст джерелаRowland, Meng Meng. "Chemical Tools to Characterize Membrane-Protein Binding Interactions Using Synthetic Lipid Probes." 2011. http://trace.tennessee.edu/utk_graddiss/1019.
Повний текст джерелаSmith, Matthew Daniel. "Chemical approaches for the investigation of protein-lipid binding interactions synthesis, modification, and evaluation of novel azido-based lipid probes /." 2009. http://etd.utk.edu/2009/Spring2009Dissertations/SmithMatthewD.pdf.
Повний текст джерелаCheema, Manpreet Kaur. "Design and synthesis of FRET-based boronic acid receptors to detect carbohydrate clustering and development of diacylglycerol-based lipid probes to investigate lipid-protein binding interactions." 2009. http://trace.tennessee.edu/utk_gradthes/517.
Повний текст джерелаSerwa, Remigiusz. "Synthesis and antioxidant properties of vitamin B₆ derivates; and [omega]-alkynylated fatty acids as substrates for preparation of modified phospholipids, novel probes for evaluating lipid-protein interactions." Diss., 2008. http://etd.library.vanderbilt.edu/ETD-db/available/etd-12182007-154611/.
Повний текст джерелаLeng, Xiaoling. "Insights on PUFA-containing lipid membranes probed by MD simulations." Thesis, 2017. https://doi.org/10.7912/C2CS96.
Повний текст джерелаThe cell membrane serves as a barrier between the interior and exterior of a living cell. Its main structural component is the lipid bilayer, which is composed of various kinds of lipids that segregate into domains. These lipid domains, distinguished in composition and physical properties from the bulk lipids that surround them, are believed to modulate the function of resident proteins by providing an appropriate lipid environment. Polyunsaturated fatty acids (PUFA) are a type of fatty acid that contain multiple C=C double bonds. They have a lot of health benefits, which may originate in part due to their incorporation into lipids in the plasma membrane. Hypotheses that PUFA-containing lipids laterally separate into domains and/or modulate the structure of existing domains have been raised to explain the fundamental role played by PUFA. In our research, we use molecular dynamics (MD) simulations to simulate model membranes composed of PUFA-containing phospholipids and to investigate their interaction with cholesterol and vitamin E that are influential membrane constituents. The presumptive function for vitamin E in membranes is to protect PUFA against oxidation. Although the chemistry of the process is well established, the role played by the molecular structure that we address with atomistic molecular dynamics (MD) simulations remains controversial. We compared the behavior of vitamin E in lipid bilayers composed of 1-stearoyl-2-docosahexaenoylphosphatidylcholine (SDPC, 18:0-22-6PC) and 1-stearoyl-2-oleoylphosphatidylcholine (SOPC, 18:0-18:1PC) via all-atom MD simulations at 37° C. SDPC represents a PUFA-containing lipid, and SOPC serves as monounsaturated control. From the calculation of van der Waals energy of interaction between vitamin E and fatty acid (FA) chains, we found higher probability that the PUFA chains surround the chromanol head group on vitamin E. This is further demonstrated by probability density maps of acyl chains around vitamin E molecules. Also, an ability to more easily penetrate deep into the PUFA containing bilayer of vitamin E is detected by faster flip-flop rate of vitamin E observed in the SDPC bilayers. These results showed that the high disorder of polyunsaturated docosahexaenoic acid (DHA) chains allows vitamin E to easily tunnel down into the bilayer and often brings the PUFA chains up to the surface of the bilayer, improving the likelihood that the reactive (hydroxyl) group on vitamin E would encounter a lipid peroxyl radical and terminate the oxidation process. Thus, the simulations indicate that the molecular structure of vitamin E supports its role as an antioxidant in a PUFA-containing membrane. A subsequent study on the partitioning of vitamin E into PUFA-containing lipids was done by analyzing the binding energy of vitamin E in the corresponding lipid bilayer. The binding energy is obtained from the potential of mean force (PMF) profile of vitamin E alone the membrane normal direction (z), which is calculated from umbrella sampling MD simulations. We found the binding in SDPC is smaller in SOPC, indicating that vitamin E does not prefer PUFA-containing phospholipids. The flip-flop rate was also estimated from the PMF profile, confirming that vitamin E flip-flops across the SDPC bilayer more easily than the SOPC bilayer. From the simulations it was noted that the membrane deforms as vitamin E is pulled out, which suggests interactions between the phospholipids contribute to the binding energy of the vitamin E. In a final study, a comparison was made between the effect on membrane organization of the three types of long chain omega-3 (n-3) PUFA found in fish oils: eicosapentaenoic acid (EPA, 20:5), DHA (22:6) and docosapentaenoic acid (DPA, 22:5). MD simulations were run on lipid bilayers composed of 1-stearoyl-2-eicosapentaenoylphosphatidylcholine (EPA-PC, 18:0-20:5PC), 1-stearoyl-2-docosapentaenoylphosphatidylcholine (DPA-PC, 18:0-22:5PC), SDPC (DHA-PC, 18:0-22:6PC) and, as a monounsaturated control, SOPC (OA-PC, 18:0-18:1PC) in the absence and presence of cholesterol. By analyzing the physical properties such as membrane order and thickness, we found all three n-3 PUFAs disorder the membrane. The disordering is greatest with EPA and least with DPA. Unique among the n-3 PUFA-containing membranes, there is region of high order in the upper portion of the DPA chain. The PUFA-containing lipids were found to less favorably interact with cholesterol compared to the OA-containing lipid, which is caused by their disorder. We speculate that differences between DPA, DHA and EPA might potentially modulate their effect on lipid domain formation.
Tzu-HaoChen and 陳子豪. "Influence of Probe-Lipid Interactions on Rupture Mechanism of Lipid Bilayer in Indentation Test: A Molecular Dynamic Simulation Study." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/94868187427233068873.
Повний текст джерела國立成功大學
材料科學及工程學系碩博士班
101
We study the indentation test of a free-standing lipid bilayer by molecular dynamics simulation, and discuss the effect of the interaction between the probe and the lipids on the bilayer structure. By calculating the local physical quantities of the lipid bilayers in the indentation process, we confirmed that the cause of bilayer rupture is not only because bilayer local thinning make the free energy of the lipid tails increase, bilayer local extension also make the lipid tails exposure to solvent and further increase the free energy of the lipid tails. These two reasons both increase the probability of micro voids formation of the lipid bilayers. In the retraction process, we found that if the interaction between the probe and the lipid tails is insufficient to make lipids adsorbed on the probe, the lipid molecules tend to rearrange to form hydrophilic bilayer edges in the rupture regions and eventually leave a hydrophilic pore in the bilayer, and no residual lipids were found on the probe. Conversely, if the lipid tails are adsorbed by the probe in the retraction process, the lipid bilayers can revert to a free-standing state, and the physical quantities of the lipid bilayers before and after the indentation are almost identical. Besides, we found that there are some residual lipids were adsorbed by the probe, and the number of the residual lipids depends on the interaction between the probe and the lipid head groups, the stronger the interaction is, the more number of residual lipids were adsorbed by the probe. These results can help us to find a suitable pharmacologically nanoinjectors. Furthermore, we investigated the relationship between the force-indentation curve and the interaction between the probe and the lipids. As expected, we observe that when the interaction between the probe and the lipid tail is stronger, the lager maximum attractive force can be measured in the retraction process at the same indentation speed. These results show that we can use the force –indentation curve obtained from AFM experiments to define the magnitude of the interaction between the probe and the lipids, and help us to select the proper probe materials in the relevant AFM experiments.
Sun, Tao. "Mass Spectrometry Applied to Problems in Lipid Biochemistry: Microchip Based Approach for Lipidomics Profiling and Analysis of Lipid Metabolites by LC-MS/MS." 2012. http://digital.library.duq.edu/u?/etd,154097.
Повний текст джерелаBayer School of Natural and Environmental Sciences
Chemistry and Biochemistry
PhD
Dissertation
Chiantia, Salvatore [Verfasser]. "Protein-lipid interactions in raft exhibiting membranes probed by combined AFM and FCS / vorgelegt von Salvatore Chiantia." 2008. http://d-nb.info/990344495/34.
Повний текст джерелаCheema, Manpreet Kaur. "Design and Synthesis of FRET-Based Boronic Acid Receptors to Detect Carbohydrate Clustering and Development of Diacylglycerol-Based Lipid Probesto Investigate Lipid-Protein Binding Interactions." 2009. http://trace.tennessee.edu/utk_gradthes/517.
Повний текст джерелаJohnson, Merrell A. "Near-Field Investigations of the Anisotropic Properties of Supported Lipid Bilayers." 2012. http://hdl.handle.net/1805/2863.
Повний текст джерелаThe details of Polarization Modulation Near-Field Scanning Optical Microscopy (PM-NSOM) are presented. How to properly calibrate and align the system is also introduced. A measurement of Muscovite crystal is used to display the capabilities of the setup. Measurements of supported Lβʹ 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) lipid bilayers are presented, emphasizing how it was tooled in exploiting the anisotropic nature of the acyl chains. A discussion of how the effective retardance (ΔS = 2π( n_e-n_o )t/λ) and the direction of the projection of the acyl chains (θ) are measured simultaneously is given, (where t is the thickness of the bilayer and λ is the wavelength of light used). It is shown from ΔS the birefringence (ne-no) of the bilayer is determined, by assuming the acyl chain tilt with respect to the membrane's normal to be ϕ ≈ 32. Time varying experiments show lateral diffusions of ~ 2 x 10-12 cm2/s. Temperature controlled PM-NSOM is shown to be a viable way to determine the main phase transition temperature (Tm) for going from the gel Lβʹ to liquid disorder Lα state of supported DPPC bilayers. A change of ΔS ~ (3.8 +/- 0.3 mrad) at the main phase transition temperature Tm (≈41^o C) is observed. This agrees well with previous values of (ne-no) and translates to an assumed <ϕ> ~ 32^o when T < Tm and 0^o when T > Tm. Evidence of supper heating and supper cooling will be presented, along with a discussion of the fluctuations that occur around Tm. Finally it is shown how physical parameters such as the polarizability are extracted from the data. Values of the transverse (αt) and longitudinal (αl) polarizabilites of the acyl chains are shown to be, αt = 44.2 Å3 and αl = 94.4 Å3, which correspond well with the theoretical values of a single palmitic acid (C16) αt = 25.14 Å3 and αl = 45.8 Å3.
Al-Abdul, Wahid Mohamed Sameer. "Oxygen as a paramagnetic probe for nuclear magnetic resonance: Structure and paramagnetic profile of a lipid bilayer/membrane model system." 2005. http://link.library.utoronto.ca/eir/EIRdetail.cfm?Resources__ID=369754&T=F.
Повний текст джерелаHong, Wen-Jie, and 洪文傑. "To Probe into the Process of Mei-Gin and the Effects on Serum Lipids of Hamsters." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/01794251480762349447.
Повний текст джерела國立中興大學
食品科學系
93
Abstract Marketing Mei-Gin is a highly concentrated and dark black product made from squeeze juice of premature mei (Japanese apricot) via filtration. This product has been generally recognized as a food with several functional properties. In this study, Taiwan mei was used as raw materials to make Mei-Gin by traditional method which combined heating and concentration. Changes in quality during processing were observed and functional property for the final Mei-Gin was investigated by biological test of rat feeding. The results were as follows. 1. Heating and concentration were two necessary processes to obtain Mei-Gin of traditionally viscous and dark black appearance. The solution of diluted Mei-Gin which was concentrated to 10-fold in acidity by combined heating and concentration and then re-hydrated to original juice showed 46.95 for total color difference (ΔE) value. The value was quite high compared to that of circulated heating at normal pressure (3.62) and vacuum concentration (3.19). 2. Scavenging efficiency of DPPH for mei juice gradually increased with heating and concentration. The final Mei-Gin product was 33.24% higher than original juice, while no obvious changes for circulated heating at normal pressure method and little decrease for vacuum concentration method. The sediment fraction obtained from the diluted Mei-Gin solution by centrifugal fractionation showed 32~53% higher in DPPH scavenging efficiency original juice while decrease was observed for supernatant fraction. The changes became much more obvious with heating and concentration indicated that availably functional components were further produced during processing. 3. Diets mixed high cholesterol (0.2% cholesterol) with various concentrations (0.05%, 0.5%, 5%) of Mei-Gin were feed to rats. The blood biochemical assessment after 2, 4, and 8 weeks showed: No regular changes in both GPT and GOT; Total cholesterol (TC) reduced from 210.92 to about 183 mg/dL at the 4th week, however, the decrease was not proportional to the concentrations Mei-Gin and was not significantly different with that of the 2nd and 8th week; Total triglyceride (TG) obviously related to concentration of Bainiku-ekisu, it reduced from 115.03 to 70.75 mg/dL (closed to the normal group, 69.67 mg/dL) for the 2nd week, while from 119.32 to 47.2 mg/dL for the 4th week, and from 141.83 to 53.8 mg/dL for the 8th week; No difference was observed in HDL for the 2nd week, but obviously increased for both 4th and 8th week though was not in proportion to the Mei-Gin concentrations; There were no differences in LDL for any experimental groups at any weeks.
Sinn, Natalie. "Omega-3 fatty acids, micronutrients and cognitive and behaviour problems associated with child attention deficit hyperactivity disorder." 2006. http://arrow.unisa.edu.au:8081/1959.8/46377.
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