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Статті в журналах з теми "Legionella pneumophila Dot"
Berk, Sharon G., Gary Faulkner, Elizabeth Garduño, Mark C. Joy, Marco A. Ortiz-Jimenez, and Rafael A. Garduño. "Packaging of Live Legionella pneumophila into Pellets Expelled by Tetrahymena spp. Does Not Require Bacterial Replication and Depends on a Dot/Icm-Mediated Survival Mechanism." Applied and Environmental Microbiology 74, no. 7 (February 1, 2008): 2187–99. http://dx.doi.org/10.1128/aem.01214-07.
Повний текст джерелаTakamatsu, Reika, Eriko Takeshima, Chie Ishikawa, Kei Yamamoto, Hiromitsu Teruya, Klaus Heuner, Futoshi Higa, Jiro Fujita та Naoki Mori. "Inhibition of Akt/GSK3β signalling pathway by Legionella pneumophila is involved in induction of T-cell apoptosis". Biochemical Journal 427, № 1 (15 березня 2010): 57–67. http://dx.doi.org/10.1042/bj20091768.
Повний текст джерелаKozak, Natalia A., Meghan Buss, Claressa E. Lucas, Michael Frace, Dhwani Govil, Tatiana Travis, Melissa Olsen-Rasmussen, Robert F. Benson, and Barry S. Fields. "Virulence Factors Encoded by Legionella longbeachae Identified on the Basis of the Genome Sequence Analysis of Clinical Isolate D-4968." Journal of Bacteriology 192, no. 4 (December 11, 2009): 1030–44. http://dx.doi.org/10.1128/jb.01272-09.
Повний текст джерелаSantic, Marina, Rexford Asare, Miljenko Doric, and Yousef Abu Kwaik. "Host-Dependent Trigger of Caspases and Apoptosis by Legionella pneumophila." Infection and Immunity 75, no. 6 (April 9, 2007): 2903–13. http://dx.doi.org/10.1128/iai.00147-07.
Повний текст джерелаHovel-Miner, Galadriel, Sergey Pampou, Sebastien P. Faucher, Margaret Clarke, Irina Morozova, Pavel Morozov, James J. Russo, Howard A. Shuman та Sergey Kalachikov. "σS Controls Multiple Pathways Associated with Intracellular Multiplication of Legionella pneumophila". Journal of Bacteriology 191, № 8 (13 лютого 2009): 2461–73. http://dx.doi.org/10.1128/jb.01578-08.
Повний текст джерелаAurass, P., B. Pless, K. Rydzewski, G. Holland, N. Bannert, and A. Flieger. "bdhA-patD Operon as a Virulence Determinant, Revealed by a Novel Large-Scale Approach for Identification of Legionella pneumophila Mutants Defective for Amoeba Infection." Applied and Environmental Microbiology 75, no. 13 (May 1, 2009): 4506–15. http://dx.doi.org/10.1128/aem.00187-09.
Повний текст джерелаZusman, Tal, Gal Yerushalmi, and Gil Segal. "Functional Similarities between the icm/dot Pathogenesis Systems of Coxiella burnetii and Legionella pneumophila." Infection and Immunity 71, no. 7 (July 2003): 3714–23. http://dx.doi.org/10.1128/iai.71.7.3714-3723.2003.
Повний текст джерелаVincent, Carr D., Benjamin A. Buscher, Jonathan R. Friedman, Lee Anne Williams, Patrick Bardill та Joseph P. Vogel. "Identification of Non-dot/icm Suppressors of the Legionella pneumophila ΔdotL Lethality Phenotype". Journal of Bacteriology 188, № 23 (22 вересня 2006): 8231–43. http://dx.doi.org/10.1128/jb.00937-06.
Повний текст джерелаNewton, Hayley J., Fiona M. Sansom, Vicki Bennett-Wood, and Elizabeth L. Hartland. "Identification of Legionella pneumophila-Specific Genes by Genomic Subtractive Hybridization with Legionella micdadei and Identification of lpnE, a Gene Required for Efficient Host Cell Entry." Infection and Immunity 74, no. 3 (March 2006): 1683–91. http://dx.doi.org/10.1128/iai.74.3.1683-1691.2006.
Повний текст джерелаWood, Rebecca E., Patrice Newton, Eleanor A. Latomanski, and Hayley J. Newton. "Dot/Icm Effector Translocation by Legionella longbeachae Creates a Replicative Vacuole Similar to That of Legionella pneumophila despite Translocation of Distinct Effector Repertoires." Infection and Immunity 83, no. 10 (July 27, 2015): 4081–92. http://dx.doi.org/10.1128/iai.00461-15.
Повний текст джерелаДисертації з теми "Legionella pneumophila Dot"
ANTÃO, Ana Catarina Ramos Batista. "Infeção de culturas primárias de monócitos humanos com a bactéria Legionella pneumophila." Master's thesis, Instituto de Higiene e Medicina Tropical, 2016. http://hdl.handle.net/10362/21860.
Повний текст джерелаLegionella pneumophila is a gram-negative bacillus that can invade and multiply in eukaryotic cells, including human monocytes and epithelial cells. Legionella pneumophila has the ability to manipulate cellular processes, with this being one of the key mechanisms that the bacteria utilizes to proliferate within the host. Infection by Legionella may present itself clinically in two different ways, Legionnaires' disease, causing severe pneumonia and Pontiac fever, or by a self-limiting bronchial infection. The phases of the bacterial life cycle and the factors that promote replication within the phagocytic cell and its transmission to a new host have been the subject of numerous studies using different species of amoebae and continuous lines, however, there are few studies on primary monocytic. The main objective of this work was to study the process of replication of Legionella pneumophila in primary monocyte lines (obtained from peripheral blood donated voluntarily) and macrophages (obtained by differentiation of primary cultures of human monocytes). To obtain a primary culture it is required to obtain a large amount of peripheral blood polymorphonuclear cells, with each isolation being influenced by the age and gender of the donor. After the infection of primary monocytes and macrophage lines with Legionella pneumophila, changes were observed in the morphology of infected cells. This observation was initially confirmed by the quantification of bacteria at 24h, that is, the comparison of the values obtained in the plating of the supernatant the lysate from the monolayer pointed to phagocytosis. However, comparing the growth of bacteria at 24h and 48h after infection, it appears that there was no intracellular multiplication.
Chedid, Maria Bernadete Fernandes. "Incidência de infecção por Legionella pneumophila em pacientes que internaram no HCPA com pneumonia adquirida na comunidade." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2002. http://hdl.handle.net/10183/3761.
Повний текст джерелаIntroduction: Legionella infections are difficult to diagnose, because the bacteria is not seen at Gram stain and the sputum culture is not performed at most laboratories. Besides that, the direct fluorescent fluorescent antibody test of respiratory secretion has low sensitivity (40%) and detection by PCR techiques is still not recommended for clinical diagnosis (CDC, 1997). The most used test is antibody detection by immunofluorescence technique or by ELISA, with a demonstration of fourfold or greater rise in the reciprocal immunofluorescente antibody (IFA) titer to greater than or equal to 1:128 against Legionella pneumophila serogroup 1 between paired acute-and convalescent-phase serum specimens, which sensitivity ranges between 70 - 80% (Edelstein, 1993).Case definitions for Legionnaires´disease agreed that patients with pneumonia who have positive results in urinary antigen assays or positive results in the direct fluorescent antibody (DFA) staining of respiratory secretions, had “probable” or presumptive” disease (WHO; 1990), as well as those who have single antibody titers of ≥1:256 (CDC, 1990). The Legionella urinary antigen test have been increasingly used in the last years, showing patients with positive results despite of negative culture tests or non-diagnostic serologies. Since then, the urinary antigen test has became a valuable tool in the prompt diagnosis of Legionnaires´disease, and also a definitive criterion for the diagnosis of Legionella pneumonias (CDC, 1997). Due to its high sensitivity, in the range of 86% to 98% (Kashubba & Ballow, 1986; Harrison & Doshi, 2001), it has been recommended to the diagnosis of community-acquired pneumonia which requires hospitalization (Mulazimoglu & Yu, 2001; Gupta et al, 2001), mainly in the ICU (ATS, 2001). Concerning to the “presumptive” criterion of single antibody title of 1:256, in the absence of seroconversion, it was concluded that it shall not be used except in the outbreak setting, since it has been reported to have low predictive value (Plouffe et al, 1995); and has also low specificity (CDC, 1997), since it has been reported high prevalence of positive antibodies at 1:256 in healthy populations (Wilkinson et al, 1983; Nichol et al, 1991). Legionnaires´disease is markedly undiagnosed, either its incidence underestimated. In several studies of CAP conducted in the USA, Europe, Israel and Australia the proportion of pneumonias caused by Legionella has ranged from 1% to 16% (Muder & Yu, 2000). In USA, the incidence of Legionella CAP in patients requiring hospitalization is estimated between 8000 to 23 000 cases per year (Marston et al, 1994 ; Marston et al, 1997). Such incidence in Brazil has not yet been estimated, being an important issue to study Objective : our goal is to detect the incidence of Legionella CAP in patients requiring hospitalization for a year, at the HCPA. Material and Methods: a cohort study ( an incidence study) of adult patients with CAP who were hospitalized for one year ( from 2000-2001) at HCPA. All patients with age 18≥80 were screened for study entry except: residents in institutions, those disabled to walk, those who had been discharged from hospital in the last 15 days; either pregnant women, HIV-positives, or patients with estructural lung diseases (bronchiectasis, cistic fibrosis) or tracheostomized. Admission logs were screened daily from Monday trough Friday (including the ones who had been hospitalized in the week-end) by the researchers. Patients with an admission diagnosis either of pneumonia or acute respiratory failure were evaluated daily by the researchers, and enrolled if they had a Chest X-Ray taken within 48 hours of admission revealing a new infiltrate consistent with pneumonia and at least 1 of the following “ major criteria” : fever (axillary temperature ≥37,8ºC), cough, or sputum; or 2 of the following “minor criteria”: dyspnea, abnormal mental status, signs of consolidation by examination, pleuritic chest pain or abnormal white blood cell count (> 12.000/cm3 or band forms > 4 % ). Information about risk factors, symptoms and outcome was collected through interview and medical chart review. Urine and serum samples were collected from consenting individuals during the acute fase at the hospital. After discharge, they came to the research ambulatory to consultation 4 to 12 weeks after patient enrollment, when the research doctor asked a new Chest X-Ray and serum sample of the convalescent phase to antibody test, along with other necessary exams. All the survivors were followed for a whole year after their inclusion in the study. Acute and convalescent sera were stored at – 70ºC and sent in dry ice (in a “batch”) to the Infectious Diseases laboratory of University of Louisville ( KY, USA), where they were tested by indirect immunofluorescent assay to IgG, IgM, and IgA antibodies to L pneumophila serogroups1-6, starting at dilution of 1:8. It was used a kit test manufactured by Zeus Scientific, Inc (Raritan, NJ, USA). All the urine samples collected were immediately frozen at –70ºC to be further tested in batches, at the Research lab of HCPA, by the investigators, with a commercial EIA kit test manufactured by Binax (BINAX Legionella Urinary Enzyme Assay). The positive ones were refrozen and further sent in a “batch” to the American laboratory, to be retested by the same kit test. Patients were diagnosed as having definite infection by L pneumophila serogroups 1-6 either if they had a 4-fold rise in antibody titer to at least 1:128 or greater dilution; or if they had positive urinary antigen, performed at our lab as recommended by the manfacturer and by the literature. A comparison was made between the patients who died and the survivors, regarding his clinical and laboratory features. Testing procedures to detect significant differences between groups included the Pearson chi-squared test or Fisher exact tests for categorical variables and Student´s t-test for continuous variables. Associations were considered statistically significant if the p value was < 0,05, using a 2-tailed test (SPSS program, version 10). Death by pneumonia was definite as the patient who died primarily due to the worsening of his lung sickness; thus, was calculated the frequency of deaths in our population. Patients who improved and were discharged, were classified as “cured”. Finally, we calculated the cumulative incidence of CAP caused by Legionella pneumophila serogroups 1-6 in a general hospital, for a year. Results: during a whole year, from 645 hospital admission logs with the diagnosis of pneumonia or acute respiratory failure screened, only 82 cases of CAP were obtained. During the follow up in the hospital or ambulatory, 23 patients were excluded either because Chest X-Ray failed to show a new pulmonary infiltrate (5 patients), alternative diagnosis were made (COPD, 5 patients; heart failure, 3; tuberculosis, 2; colagenosis, 1; idiopathic pulmonary fibrosis, 1). Aditional 6 patients revealed exclusion criteria as being HIV positive (1 patient), to have bronchiectasis (4) or pneumatocele (1). Thus, 59 patients constituted the final study group, being each patient enrolled only once. The mean age was 57,6 years (ranging from 24 to 80), being 20 women and 39 men. Most of them ( 36 patients, 61%) had chronic underlying diseases; half of them had more than one disease, being more prevalent: lung diseases, heart diseases, diabete mellitus, liver diseases and renal failure. Regular cigarette smokers represented 61% of the total, and alcohhol intake, 16,9%. Cancer ocurred in 9 patients, being solid organ malignancy in 7 and haematologic malignancy in 2. From our 59 patients, 10 were classified as immunossupressed, defined as splenectomy, haematological malignancy, autoimmune disease, transplant recipient, cancer chemotherapy within 4 weeks (Yu et al, 2002), or prednisolone use ≥10 mg/day (or equivalent), for at least 3 months before admission (LIM et al, 2001). In the remaining 13 patients, only one was previously healthy, while the others had sinusitis, anemia, hypertension, or other mild diseases. At admission, Chest X-Ray showed intersticial pneumonia in only one patient; bronchopneumonia in 59,3% and airspace pneumonia in 23,7%, while both patterns ocurred concomitantly in 15,2%. Obstructive pneumonia (Fang et al, 1990) ocurred in 5 patients with lung cancer. Pleural effusion ocurred in 22%, and in 21 patients (35%) the presentation was multilobar.The antibiotic class most used were beta-lactams, in 72,9% of the patients. The remaining received at most respiratory quinolones and macrolides. From the group that used beta-lactams, 25 patients did not use either quinolones or macrolides.There were not statistic differences in mortality regarding age, sex, or treatment between the groups who received beta-lactams alone versus the group that received macrolides or respiratory quinolones. The only significant association ocurred between radiographic pattern of airspace pneumonia and greater mortality (p= 0,05). In this study 3 patients had pneumonia caused by Legionella pneumophila serogroup 1: 2 patients had seroconversion and positive antigen urinary test; the third patient had a positive urinary antigen with negative serologies, like some authors (McWHINNEY et al, 2000). The former two patients worsened with beta-lactams, prescribed before the etiological diagnosis, getting resolution of their pneumonia with levofloxacin; the third one used only beta-lactams, getting cure. There were 7 deaths for pneumonia, and 4 deaths for cancer. From 48 survivors, 33 patients (68,7%) were alive after 12 months. Our mortality rate (13,5%) is similar to the one reported in the literature (ATS, 2001). Conclusions: the incidence of hospitalized CAP by Legionella pneumophila serogroups 1-6 in our hospital in the year 2000-2001 was 5,1%, which represents the annual incidence of Legionnaires´ disease in a general hospital of South Brazil. Comments and perspectives: complementary diagnostic methods like culture, serologies to detection of all classes of immunoglobulins and urinary antigen tests shall be used to detect infections by Legionella in our country to detect the real incidence of pneumonias caused by Legionella species. At the moment, the Legionella antigen test has the greatest yeld among the available tests. It is recommended to all hospitalized PAC patients (Mulazimoglu &Yu, 2001; Gupta et al, 2001); and also to all patients who have potential risk factors for legionellosis (Marrie, 2001), as well as to the etiological diagnosis of severe pneumonias (ATS, 2001). Its use is recommended, with unanimity, to the diagnosis of community and nosocomial outbreaks.
Lopes, Sandro Ribeiro. "Functional diversity of Legionella pneumophila Dot/Icm Effector SdhA in Galleria mellonella model." Master's thesis, 2017. http://hdl.handle.net/10316/83085.
Повний текст джерелаLegionella pneumophila é o principal agente da Doença dos Legionários, uma pneumonia severa e ocasionalmente mortal. Esta bactéria gram negativa, é ubíqua em ambientes aquáticos não salinos e em sistemas artificiais de água, replicando-se dentro de protozários aquáticos (principalmente amebas), mas também dentro de macrófagos alveolares humanos.A virulência de L. pneumophila depende da sua capacidade de remodelar o vacuolo fagocítico, denominado “Legionella-containing vacuole” (LCV), para criar um nicho replicativo prevenindo a fusão do fagossoma com o lisossoma, evitando assim o sistema imunitário do hospedeiro. Para realizar estas tarefas, L. pneumophila efectua a translocação de numerosos efectores bacterianos para a célula hospedeira através do sistema de secreção Dot/Icm Tipo IV (T4BSS). Este sistema de secreção é responsável pela translocação um vasto número de efectores que modulam diversas atividades da célula hospedeira. Apesar do elevado número de efetores identificados, apenas alguns são considerados críticos para o crescimento intracelular da bactéria, como a proteína SdhA. Esta proteína é importante para a manutenção da integridade do LCV em macrófagos, uma vez que na sua ausência o LCV é fragmentado resultando na morte da célula hospedeira e da bactéria.Nos últimos anos o uso de Galleria mellonella como um modelo de infeção para o estudo de agentes patogénicos tem aumentado devido a existência de uma correlação entre a virulência de bactérias patogénicas em insetos e nos modelos em mamíferos. Neste inseto, a mortalidade induzida por L. pneumophila é dependente da dose e do sistema de secreção Dot/Icm T4BSS funcional. Para além disso, a utilização de G. mellonella na determinação da função e relevância de efetores do Dot/Icm T4BSS, como o SdhA, na virulência desta bactéria já foi demonstrada.O objetivo principal deste estudo foi determinar se o efetor SdhA translocado por Dot/Icm T4BSS e crucial para a virulência de L. pneumophila em G. mellonella, é está envolvido em diferenças de virulência em estirpes de L. pneumophila não relacionadas, isoladas de diferentes ambientes e com origens genéticas distintas, usando a larva G. mellonella como um modelo de infeção.Neste estudo verificámos que a virulência da maioria das estirpes de L. pneumophila é dependente do efetor SdhA. Para além disso, a relevância deste efector na infeção por L. pneumophila variou entre as estirpes analisadas. Assim, concluímos que o efector SdhA é responsável pelos níveis distintos de virulência observados entre estirpes de L. pneumophila isoladas de ambientes distintos e com diferentes contextos genéticos. Adicionalmente, detetámos pela primeira vez a existência de redundância funcional para SdhA na infeção em G. mellonella entre estirpes de L. pneumophila
Legionella pneumophila is the major agent of Legionnaire´s Disease (LD) a severe and occasionally fatal pneumonia. This gram negative bacteria that is ubiquitous in freshwater environments and in many man-made water systems, replicates within aquatic protozoa (mainly amoeba), but also within human alveolar macrophages.L. pneumophila virulence depends on the ability to use the phagocytic vacuole, namely Legionella-containing vacuole (LCV), to create a replicative niche preventing phagosome-lysosome fusion and evade the host immune system. To accomplish these tasks L. pneumophila translocate numerous bacterial effectors into the host cell though Dot/Icm Type IV Secretion System (TB4SS). This secretion system is responsible for the translocation of vast number of effectors that modulate diverse host cell functions. Despite this large number of recognized effectors only a few are considered to be critical for intracellular growth and disease, such as SdhA protein. This protein is crucial for the maintenance of LCV integrity in macrophages, since in the absence of this effector occurs the LCV disruption resulting in the death of both host cell and bacterium.An increase in use of G. mellonella as an infection model for human pathogens occurred in the last year due the existence of a large correlation between virulence of bacterial pathogens in the insect and in mammalian models. In this insect, mortality induced by this L. pneumophila is both dose and functional Dot/Icm T4BSS-depedent. Moreover, the suitability of G. mellonella to determine the role of Dot/Icm T4BSS effectors, such SdhA, in virulence of this bacterium was already demonstrated.The main objective of this study was to determine if the role of the crucial virulence-related Dot/Icm T4BSS effector SdhA induce different levels of virulence among unrelated L. pneumophila strains, isolated from different environments and with distinct genetic backgrounds, using G. mellonella larvae as an infection model.In this study the majority of L. pneumophila strains induced a sdhA-dependent larval mortality. In addition, relevant differences on the role of sdhA were observed among the studied strains. In sum, SdhA induced different levels of virulence among unrelated L. pneumophila strains in G. mellonella infection. Importantly, some degree of functional redundancy towards SdhA was detected for the first time in a L. pneumophila strain.
Chasqueira, Maria de Jesus Fernandes. "Doença dos legionários : estudo da diversidade de isolados de legionella obtidos em Portugal, 1987-2016." Doctoral thesis, 2017. http://hdl.handle.net/10362/22302.
Повний текст джерелаABSTRACT: The work developed was divided into two different areas of knowledge of the genus Legionella: epidemiology and natural bacterial-host interaction. The epidemiology of Legionnaires‘ disease was studied in Portugal between 1987 and 2016, analyzing 205 isolates, 178 of which recovered from patients with severe forms of disease and 27 from environmental samples. Among the clinical isolates, 130 were sent by the Program of Integrated Epidemiological Surveillance of Legionnaires' Disease and 48 were recovered in a hospital in the Lisbon area, with several cases of hospital infection for 21 years, and with systematic isolation over the years in the water of the distribution system. For typing of these isolates, two methodologies recommended by the European Group, the monoclonal antibodies (MAbs) of the Dresden Panel and the sequence-based typing (SBT) were used. In the group of isolates from cases of hospital infection, two other methods were applied, amplified fragment length polymorphisms (AFLP) and whole genome sequencing (WGS). The typing results showed that all isolates belong to the species Legionella pneumophila and mainly to serogroup 1, and all but one reacts with the monoclonal MAb3/1. In sequence-based typing (SBT), 39 different profiles were identified, 16 of which were new profiles, therefore never previously identified. In the whole genome sequencing, of the 48 isolates from hospital infection, it was possible to group them in the same clone, with a microevolution marked essentially by the fixation of point mutations. Among the isolates, it was possible to identify three sub-lineages, based on the number of nucleotide differences. The direct characterization on clinical samples by a nested PCR technique allowed the identification of some alleles, and it was verified that only in samples from supernatants of amoeba cultures the complete allelic profile was detected. It was also carried out the study of the phylogenetic relationship between the allelic profiles identified in Portugal and those reported to the European Database by other countries. The population of Legionella responsible for cases of disease in Portugal consists of a mixture of specific profiles (exclusive of Portugal) plus profiles common to other countries, and it was verified in this evaluation that 34 of the profiles have relation with at least one profile of the European Database. In the second part of this thesis, the study of the interaction Legionella-natural host was developed, using the species Acanthamoeba castellanii. The rates of internalization and multiplication were evaluated at 4, 14 and 22h, sensitivity to sodium, osmotic shock with potassium, and bacterial transcriptome, 22 hours after the start of co-culture. The results showed specificity in relation to the two strains used, and although they presented a transmissible phenotype, it was verified that the pattern of gene expression is similar to that evidenced by strains in the replicative phase, suggesting that Legionella at the final phase of its intracellular multiplication cycle is already preparing the next replicative phase.
Domingues, João Pedro Antunes. "Surto de Legionella em Portugal no ano 2014 : Análise ao conhecimento da população." Master's thesis, 2015. http://hdl.handle.net/10451/26976.
Повний текст джерелаEnquadramento: Entre Outubro e Dezembro de 2014, ocorreu em Vila Franca de Xira, o surto de Legionella de maiores dimensões em Portugal, e um dos maiores da Europa, com 375 casos notificados e 12 óbitos declarados. A doença dos Legionários é uma doença respiratória, geralmente causada pela bactéria Legionella pneumophila e apresenta um quadro sintomático muito semelhante a uma pneumonia comum. Apesar de poder afetar qualquer pessoa, os homens, os fumadores, os imunocomprometidos e as pessoas com mais de 50 anos estão mais suscetíveis à infeção. A transmissão é feita por inalação de água contaminada pela bactéria, muitas vezes proveniente de torres de refrigeração e duches. Objetivos: Fazer uma recolha de informação e analisar o nível de conhecimento da população relativamente ao surto de doença dos legionários em Vila Franca de Xira, através de um inquérito à população. Métodos: Os artigos científicos foram obtidos na base de dados Pubmed, OMS e DGS. O inquérito foi elaborado na plataforma Google Forms, e disponibilizado online, através de fóruns e das redes sociais. Pontualmente, foram elaborados inquéritos por entrevista pessoal. Os dados foram tratados com recurso a Microsoft Excel e a Epi Info 7. Resultados: Numa tentativa de quantificar o conhecimento da população, atribuiu-se uma pontuação a cada resposta ao questionário, que resultou numa pontuação média de 67.53 num máximo de 100 pts. Globalmente, os inquiridos souberam responder às questões, apresentando alguma dificuldade em reconhecer que ser do género masculino é um fator de risco e que a tosse com expetoração não é um sintoma da doença. Conclusão: O conhecimento geral do surto é satisfatório, apresentando apenas algumas lacunas na seleção correta de todos os sintomas e fatores de risco.
Vasconcelos, Mafalda Margarida Pereira de. "Legionelose." Master's thesis, 2015. http://hdl.handle.net/10451/27119.
Повний текст джерелаA Doença dos Legionários ou Legionelose é uma doença provocada pela bactéria Legionella pneumophila. A sua transmissão ocorre através da inalação de aerossóis contaminados e apresenta-se sob a forma de pneumonia contraídas na comunidade ou em ambiente hospitalar. A limpeza dos sistemas de distribuição de água e refrigeração do ar constitui a principal medida preventiva para que não ocorra a transmissão da bactéria. O tratamento é efetuado através de antibioterapia, sobretudo com azitromicina. O atraso na terapêutica aumenta consideravelmente a taxa de mortalidade associada à patologia. Atualmente, a Legionelose constitui um problema de Saúde Pública que deve ser obrigatoriamente notificado. No entanto, através do questionário efetuado verificou-se que a grande maioria dos inquiridos está equivocado sobre a forma de transmissão dos bacilos e sobre a prevenção da doença. A Farmácia pela proximidade que mantém com o utente, poderá ser, por excelência, onde deverão ser ministrados de forma objetiva, clara e concisa estes esclarecimentos que em situações de surto poderão fazer a diferença numa população.
Частини книг з теми "Legionella pneumophila Dot"
Vincent, Carr D., Kwang Cheol Jeong, Jessica Sexton, Emily Buford, and Joseph P. Vogel. "The Legionella pneumophila Dot/Icm Type IV Secretion System." In Legionella, 184–91. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555815660.ch47.
Повний текст джерелаIsberg, Ralph R., and Matthias Machner. "Identification of Translocated Substrates of the Legionella pneumophila Dot/Icm System without the use of Eukaryotic Host Cells." In Legionella, 167–76. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555815660.ch45.
Повний текст джерелаSegal, Gil. "Evolution of Legionella pneumophila Icm/Dot Pathogenesis System." In Evolutionary Biology of Bacterial and Fungal Pathogens, 455–64. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555815639.ch38.
Повний текст джерелаKubori, Tomoko, and Hiroki Nagai. "Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila." In Methods in Molecular Biology, 241–47. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9048-1_15.
Повний текст джерелаZhu, Wenhan, and Zhao-Qing Luo. "Methods for Determining Protein Translocation by the Legionella pneumophila Dot/Icm Type IV Secretion System." In Methods in Molecular Biology, 323–32. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-161-5_19.
Повний текст джерелаSegal, Gil. "The Legionella pneumophila Two-Component Regulatory Systems that Participate in the Regulation of Icm/Dot Effectors." In Current Topics in Microbiology and Immunology, 35–52. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/82_2013_346.
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