Статті в журналах: "LCPI"

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Perepjolkina, Viktorija, Jeļena Koļesņikova, Kristīne Mārtinsone, and Ainārs Stepens. "DEVELOPMENT OF THE SECOND PRELIMINARY ITEM POOL OF THE LATVIAN CLINICAL PERSONALITY INVENTORY: AN INTEGRATIVE APPROACH." SOCIETY. INTEGRATION. EDUCATION. Proceedings of the International Scientific Conference 1 (May 26, 2016): 469. http://dx.doi.org/10.17770/sie2016vol1.1521.

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On the moment, there is a lack of available clinical personality assessment instruments in Latvia, so in the frame of the National Research Programme (2014-2017, project No. 5.8.2.) a new multi-item multi-scale self-report measure - the Latvian Clinical Personality Inventory (LCPI) is currently being developed. LCPI structure includes three parts: clinical scales, pathological personality scales and functioning scales and will provide a possibility to perform a combined analysis of mental disorders symptoms and functioning aspects using one clinical personality inventory. The main aim of this article to describe procedures and stages of the development of the preliminary version of the LCPI v2.2. item pool.

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Kotak, Dr Abhinav, and Dr Suresh Rudani. "Corrective Osteotomy in cubitus varus deformity in children: A prospective study." Surgical update: International Journal of Surgery and Orthopedics 7, no. 3 (June 1, 2021): 73–77. http://dx.doi.org/10.17511/ijoso.2021.i03.06.

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Background and Aim: Cubitus varus deformity is the most common late complication aftersupracondylar fracture of the distal humerus in children, incidence varying from 4% to 58%. Thepresent study was done to evaluate the results of dome osteotomy. Material and Methods: Thisprospective study was conducted in a tertiary care hospital over 16 months. In all patients humerus-elbow wrist angle was measured on both sides and the correction needed was calculated. The lateralcondyle prominence index (LCPI) was calculated by anteroposterior view radiographs of thedeformed and the normal elbow in full extension by (AB-BC)/AC. Dome osteotomy with para tricepsapproach was used. Pre and post-operative carrying angle of elbow, range of motion and lateralprominence indices were compared. Results: The age of patients ranged from 3 to 15 years with amean age of 8.47±3.14 years. Preoperative carrying angle of normal side ranged from 80 to 140and that of effected side ranged from -23 to -13 and the difference was statistically significant(p<0.05). LCPI ranged from -8.4 to 5.9%. The majority of cases had LCPI >2.7%. As compared to,an improvement in carrying angle at defect side was observed to be 28.41±2.15 which wassignificant (p<0.05). At baseline mean LCPI was 0.39±3.87% which changed to -0.86±3.47%, themean change of this was significant (p=0.01). baseline Conclusion: Dome osteotomy is a relativelytechnically demanding technique for correction of cubitus varus deformity but with a betterfunctional outcome without being associated with lateral condyle prominence.

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Yu, Xuan, Manhong Shen, Di Wang, and Bernadette Tadala Imwa. "Does the Low-Carbon Pilot Initiative Reduce Carbon Emissions? Evidence from the Application of the Synthetic Control Method in Guangdong Province." Sustainability 11, no. 14 (July 23, 2019): 3979. http://dx.doi.org/10.3390/su11143979.

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As the world’s top energy consumer and carbon emitter, China’s carbon emissions policies, including the low-carbon pilot initiative (LCPI) implemented in July 2010, have important effects on global climate change. Therefore, accurately assessing the effect of this policy has become extremely important for low-carbon development. This article analyses the impact of implementing LCPI on regional carbon emissions by using Guangdong Province as the study area, which has the largest economic scale, population size and carbon emissions amongst China’s low-carbon pilot provinces. The results suggest that for the entire 2010–2015 period, Guangdong’s carbon emissions were reduced by about 10% due to the implementation of LCPI. This policy produced a significant impact on the carbon emissions from manufacturing industries but showed minimal impact on the carbon emissions from energy production. Unlike previous researchers who relied on estimations, the authors of this work obtained unified carbon emissions data for 1997–2015 from the China Emission Accounts and Datasets and then constructed comparison groups by using the synthetic control method instead of performing a subjective selection. The authors also examined the impact of LCPI on carbon emissions from different sources. This article proposes that policy support and low-carbon action are necessary for reducing regional carbon emissions and that the policies must be constantly adjusted during their implementation. The successful experiences in low-carbon pilots are also worth exploring and promoting in other regions.

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Charles, Jean-Philippe, Carol Chihara, Shamim Nejad, and Lynn M. Riddiford. "A Cluster of Cuticle Protein Genes of Drosophila melanogaster at 65A: Sequence, Structure and Evolution." Genetics 147, no. 3 (November 1, 1997): 1213–24. http://dx.doi.org/10.1093/genetics/147.3.1213.

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A 36-kb genomic DNA segment of the Drosophila melanogaster genome containing 12 clustered cuticle genes has been mapped and partially sequenced. The cluster maps at 65A 5-6 on the left arm of the third chromosome, in agreement with the previously determined location of a putative cluster encompassing the genes for the third instar larval cuticle proteins LCP5, LCP6 and LCP8. This cluster is the largest cuticle gene cluster discovered to date and shows a number of surprising features that explain in part the genetic complexity of the LCP5, LCP6 and LCP8 loci. The genes encoding LCP5 and LCP8 are multiple copy genes and the presence of extensive similarity in their coding regions gives the first evidence for gene conversion in cuticle genes. In addition, five genes in the cluster are intronless. Four of these five have arisen by retroposition. The other genes in the cluster have a single intron located at an unusual location for insect cuticle genes.

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Elshafey, Ahmed, Abeer Aziza, ,. Abdelhady Orma, and Tarek Mohamed. "The effect of synthetic essential amino acids supplementation to low crude protein diets on growth performance and serum metabolites in broiler chickens." September2019 20, no. 3 (September 17, 2019): 24–29. http://dx.doi.org/10.35943/mvmj.2019.23.205.

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Objective: To assess the influence of reduction of crude protein (CP) by 5% and 10% with supplementation of crystalline synthetic essential amino acids (EAAs) on performance and carcass traits of broilers. Design: Randomized controlled study Animals: A total of 320 one-day-old broiler chicks, (Cobb 500) reared up to 35 days of age were randomly allocated into eight experimental groups with four replicates (10 each). Procedures: The treatments as follow; the first group of chicks were fed on control protein to meet the CP requirement and total AAs according to Cobb 500 recommendation; the second group were fed idle EAAs (Id EAAs) to meet the recommended CP and the digestible EAAs. The third, fourth, and fifth groups were fed on diet 5% reduction of CP with EAAs supplementation exceed the digestible EAAs requirement of Cobb by 110, 120, and 130% (LCP3+110EAAs, LCP4+120EAAs, LCP5+130EAAs). However, the sixth, seventh, eighth groups of chicks were fed on diet 10% reduction of CP with AAs supplementation exceed the digestible EAAs requirement of Cobb by 110, 120, and 130% (LCP6+110EAAs, LCP7+120EAAs, LCP8+130EAAs). Results: Broilers of group four, five, six, seven and eight showed a significant decrease final body weight (BW), body weight gain (BWG) and feed intake at 21 and 35 days of age. However, the same variables were significantly enhanced in broilers of group one, two, and three (p <0.05). Additionally, there was a significant decrease of relative weight of abdominal fat pad of broilers in groups three, four, five compared to other treatment groups. Serum uric acid and cholesterol were linearly decreased with reduction of CP and supplementation with synthetic essential amino acids. Conclusion and clinical relevance: The present results indicate that reduction of CP by 5% or 10% with crystalline EAAs supplementation by 110, 120 and 130% more than the digestible EAAs of broiler recommendation did not support the growth performance.

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Castagnini, Francesco, Giuseppe Tella, Maurizio Montalti, Federico Biondi, Barbara Bordini, Luca Busanelli, and Aldo Toni. "Mid-term outcomes of a partial 2-stage approach in late chronic periprosthetic hip infections." HIP International 30, no. 3 (January 2, 2020): 327–32. http://dx.doi.org/10.1177/1120700019855627.

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Introduction: Late chronic periprosthetic infections (LCPIs) are worrisome complications of primary hip arthroplasties. The gold standard procedure is the 2-stage revision. 1-stage exchange is gaining popularity in order to reduce the invasivity of the former technique. A partial 2-stage exchange technique, retaining fixed components, may overcome some of the drawbacks of the previous techniques, allowing a much easier reconstruction step. Methods: 28 patients with a LCPI after a primary total hip arthroplasty underwent a first removal stage: the loosened component was removed (23 cups and 5 stems) and the fixed component, with no local signs of infection, was retained. An antibiotic hand molded spacer was positioned in 16 cases. After a mean time of 8 months and a tailored antimicrobial therapy, the spacer was removed and the implant was revised. Results: The mean follow-up was 5 years. The HHS score was 82.7. 4 cases failed (2 patients presenting a septic relapse after revision and 2 patients undergoing Girdlestone arthroplasty), achieving a survival rate of 83.4% at 5 years. 2 patients were unwilling to perform a further procedure and did not proceed to the second stage. All the other patients had no clinical, radiological, laboratory signs of septic relapse. Conclusions: The partial 2-stage approach seems a promising technique for LCPI in selected cases, with good infection control. It allows an easier revision by sparing the fixed components. Larger case series and longer follow-ups are needed to confirm the results and identify the limits of this approach.

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Kolesnikova, Jelena, Viktorija Perepjolkina, Kristine Martinsone, and Ainars Stepens. "THE THEORETICAL INTEGRATIVE MODEL FOR THE LATVIAN CLINICAL PERSONALITY INVENTORY." SOCIETY. INTEGRATION. EDUCATION. Proceedings of the International Scientific Conference 1 (May 26, 2016): 388. http://dx.doi.org/10.17770/sie2016vol1.1518.

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The purpose of this article is to present a theoretical integrative model, which reflects contemporary tendencies in the understanding of mental disorders and functional impairment, and which is used as a theoretical frame for the development of the Latvian Clinical Personality Inventory (LCPI). This article, based on the latest research findings in the field, supports the necessity of a combined analysis of mental disorders and functional impairment. Due to scientific findings and deeper understanding of the interrelation between mental disorders and functioning impairment, it has become possible to develop psychological instruments for valid assessment of the disturbances in an individual's cognition, emotion regulation, and behaviour combining with analysis of most essential and relevant aspects of their functioning. An integrative theoretical model of LCPI, developed on the selected criteria from Diagnostic and Statistical Manual of Mental Disorders (DSM – 5; APA, 2013) and WHO International Classification of Functioning, Disability and Health (ICF; WHO, 2001/2015) is presented in the article. Conducted literature analysis permits to conclude, that in a context of clinical personality assessment, a combined analysis of symptoms of mental disorders and relevant functioning criteria is very promising and will be useful in many assessment contexts. Based on such an integrative approach the Latvian Clinical Personality Inventory is currently being developed. This project is a part of the National Research Programme (No. 5.8.2.).

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MAO, YAXIAN, WENCHANG XIANG, and DAICUI ZHOU. "CHARM QUARK ENERGY LOSS IN DENSE MATTER WITHIN THE LIGHT-CONE PATH INTEGRAL APPROACH." International Journal of Modern Physics E 16, no. 07n08 (August 2007): 2130–36. http://dx.doi.org/10.1142/s021830130700757x.

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The energy loss of heavy quarks traversing color dense matter is calculated with an analytical formula derived within the light-cone path integral (LCPI) approach. We find that the energy loss mechanism is dominated by gluon radiation and induces a suppression pattern of charm quark different from the suppression of light quarks. We find also that this radiative energy-loss is proportional to L2 for energetic quarks, but changes to a L dependance with decreasing quark energy, where L is the length of the traversed medium.

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Perepjolkina, Viktorija, Jeļena Koļesņikova, Kristīne Mārtinsone, Ainārs Stepens, and Elmārs Rancāns. "CRITERION-BASED VALIDITY OF THE DEPRESSION SCALE OF LATVIAN CLINICAL PERSONALITY INVENTORY." SOCIETY. INTEGRATION. EDUCATION. Proceedings of the International Scientific Conference 1 (May 26, 2017): 603. http://dx.doi.org/10.17770/sie2017vol1.2351.

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The main aim of this study was to evaluate the criterion validity and to estimate the cut-off score of the Depression scale (DS) and short Depression scale (DSs) for a new self-report measure – Latvian Clinical Personality Inventory (LCPI). Usefulness of DS and DSs for identifying patients with major depression were analysed based on psychometric analysis of data acquired from psychiatric inpatient sample with depressive disorder (n = 37) in comparison to randomised stratified community subsample (n = 176) selected from the overall test development sample (N = 888). The present study was carried within the framework of the National Research Program (BIOMEDICINE) 2014 – 2017 (sub-project Nr.5.8.2.). It was shown that all 24 item of DS show good to excellent discrimination power. Cronbach’s alpha was 0.97 for DS and 0.95 for DSs in test development sample. For DS, the optimal cut-off score was 26 points (sensitivity 95%, specificity 91%, and positive predicted value of 69%). For DSs, the optimal cut-off was 12 points (sensitivity 92%, specificity 89%, and positive predicted value 63%). DS and DSs of LCPI is proved to have good criterion validity in detecting depression and to be a reliable and valid instrument for assessment of depression symptoms in patients with depression and in general population. Subjects scoring at least 26 on DS or 12 points on DSs constitute a target group for further diagnostic assessment in order to determine appropriate treatment.

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Perepjolkina, Viktorija, Jeļena Ļubenko, Jeļena Koļesņikova, Kristīne Mārtinsone, and Ainārs Stepens. "Incremental Role of Pathological Personality Traits in the Prediction of Suicidal Ideation in General and Psychiatric Inpatient Sample." Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 73, no. 4 (August 1, 2019): 356–63. http://dx.doi.org/10.2478/prolas-2019-0055.

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Abstract The aim of this study was to evaluate the utility of the pathological personality traits in predicting suicidal ideation, especially in combination with other risk factors, such as the level of depression, prior attempts of suicide, low self-esteem, low level of perceived social support and self-esteem-by-social support interaction, both in general and in psychiatric inpatient samples. Data were analysed within two samples: non-clinical general sample (n = 461) and psychiatric inpatient sample (n = 131). Latvian Clinical Personality Inventory (LCPI) was used as the instrument for data collection. LCPI is a comprehensive multi-scale multi-item inventory, which consists of nine clinical scales (including Depression Symptom Scale), 33 pathological personality scales, five functioning scales, and five additional scales, including a Suicidal Ideation Scale, Low Self-esteem Scale and Lack of Perceived Social Support Scale. Results of the hierarchical regression analysis showed that several facet-level pathological personality traits (depressivity, self-harm, dissociation proneness, submissiveness, and suspiciousness) added significant incremental variance to the prediction of suicidal ideation above and beyond the well-known main risk factors of suicidal ideation, such as depression and prior suicide attempt. This effect remained stable even after taking into account additional interpersonal risk factors, such as low self-esteem, low level of perceived social support and self-esteem-by-perceived social support interaction. The incremental effect of personality traits was medium in the psychiatric inpatient sample and small in the general sample. Findings of the study may assist in early screening for persons with suicide risk and for developing prevention programmes in different settings.

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Jha, Ranjib Kumar, Santosh Thapa, and Dhiraj Singh. "Outcome of Corrective Dome Osteotomy for Cubitus Varus Deformity." Journal of Nobel Medical College 8, no. 2 (December 18, 2019): 42–46. http://dx.doi.org/10.3126/jonmc.v8i2.26760.

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Background: The cubitus varus deformity is one of the most common late complications of fracture supracondylar area of humerus in children. Various corrective osteotomies are used of which lateral closed wedge osteotomy is commonly done which has its own disadvantages like lateral condylar prominence, difficulty in achieving correction and limitation of movement. Dome osteotomy is a versatile technique to achieve correction of deformity in all planes and to overcome these above complications. The aim of this study was to evaluate the results of dome osteotomy for correction of post-traumatic cubitus varus deformity of elbow in children. Materials and Methods: The study included 18 children between 6-13 years of age of both sex with malunited supracondylar fracture of distal humerus having cubitus varus deformity. After appropriate pre- operative assessment, dome osteotomy was done by posterior approach. In pre and post operative x-ray carrying angle and lateral condylar prominence Index (LCPI) were calculated. Patients were re-assessed at complete union and results were calculated. Results: In this study of 18 patients, 12 were males and 6 were females. The age ranged from 6-13 yrs with average of 8.4yrs. The average correction of carrying angle was from -22.4 degree to +10 degree. LCPI changed post operatively ranging from -8.6% to +3.25%, average -2.9%. There were no significant complications. Fifteen patients had excellent outcome and 3 had good outcome. Conclusion: The results in our study concluded that dome osteotomy for cubitus varus is safe and effective method and give cosmetically more acceptable elbow.

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Duarte, Marcos Elias E., Zixiao Deng, Jonathan Baker, and Sung Woo Kim. "259 Impact of Reducing Dietary Protein with Supplementation of 8 Essential Amino Acids and Functional Roles of Supplemental his or Glu on Growth Performance and Carcass Traits of Finishing Pigs." Journal of Animal Science 100, Supplement_3 (September 21, 2022): 112. http://dx.doi.org/10.1093/jas/skac247.216.

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Abstract This study evaluated the effects of reducing dietary protein with supplementation of 8 essential amino acids and functional roles of supplemental Glu or His on growth performance and carcass traits of finishing pigs. Pigs (n = 216; 98 ± 3.5 kg BW) were allotted (3 pigs/pen) to 4 treatments in a randomized complete block design (RCBD) with sex and initial BW as blocks. Pigs were fed for 14 d until 120 kg BW. Dietary treatments were CON (11.4% CP with L-Lys, L-Thr, and L-Trp); LCP (8.9% CP with L-Lys, Thr, Trp, Met, Val, Ile, Phe, and His); LCPG (9.1% CP; LCP+Glu to match % Glu in CON); and LCPH (9.2% CP; LCP+2x His requirement). Diets met or exceeded requirements with 2.7 Mcal/kg NE. Growth performance and backfat thickness (BFT) were measured at d 0 and d 14. Blood was collected to analyze BUN. At d 14, pigs with the median BW in each pen were slaughtered. Pigs fed CON had greatest (P < 0.05) BW, ADG, and G:F. Pigs fed LCP had greater (P < 0.05) BW, ADG, and ADFI than LCPG. BFT of pigs fed LCPG and LCPH was less (P < 0.05) than CON. Cold carcass weight in CON was greater (P < 0.05) than LCPG and LCPH, whereas it was greater (P < 0.05) in LCP than in LCPG. Chilling loss in LCP was less (P < 0.05) than LCPG. Drip loss at 48 h in LCPH was less (P < 0.05) than CON and LCPG. BUN in LCPG and LCPH was less (P < 0.05) than CON. In conclusion, reducing dietary protein by 2.5% using 8 supplemental amino acids reduced growth performance without affecting carcass yield. Supplemental His at 2x requirement to low protein diets meeting His requirement enhanced loin quality by reducing drip loss.

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John, Ani, Roma Shah, William Bruce Wong, Charles Schneider, Hamid H. Gari, and Marliese Johnson. "Value of precision medicine in advanced NSCLC: Real-world outcomes associated with the use of companion diagnostics." Journal of Clinical Oncology 37, no. 15_suppl (May 20, 2019): e20015-e20015. http://dx.doi.org/10.1200/jco.2019.37.15_suppl.e20015.

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e20015 Background: Five-year survival rates as low as 2.8% have been reported in patients with non-small cell lung cancer (NSCLC), highlighting the need for individualized diagnosis and treatment. Companion diagnostic testing (CDx) identifies patients with molecular targets likely to respond better to particular therapies; however, not all cancer patients receive CDx in the real-world setting. This study evaluated the clinical value of CDx in the real world with respect to overall survival among patients with non-squamous advanced (Stage IIIB/IV) NSCLC (aNSCLC). Methods: Patients were from the Flatiron Health electronic health-derived database, treated with systemic therapy, and diagnosed with aNSCLC between January 1, 2011 and May 31, 2018; those who received CDx with their first line of treatment were compared with those who did not. Logistic regression using components of the modified Lung Cancer Prognostic Index (LCPI; age, sex, stage, actionable mutation(s), smoking, respiratory comorbidity; Alexander et al. Br J Cancer. 2017) and other factors were used to predict characteristics associated with receiving CDx. Overall survival was evaluated using Kaplan-Meier analysis. Unadjusted and adjusted Cox proportional hazards regression models were used to evaluate the association between CDx and overall survival. Results: A total of 17,143 patients with aNSCLC (CDx, n = 14,389; no CDx, n = 2754) and a mean (SD) age at diagnosis of 67.2 (10.0) years (CDx, 67.1 [10.1]; no CDx, 67.5 [9.2]) were included. There were more nonsmokers in the CDx group (17.4%) than the no CDx group (5.5%). Patients who were female, diagnosed after 2014, receiving multiple lines of therapy or had advanced stage at diagnosis were more likely to receive CDx. Patients receiving CDx had decreased mortality risk (unadjusted HR [95% CI] = 0.54 [0.52-0.57]) and lived longer than those not receiving CDx (median survival = 14 vs 7 months). The significant reduction in mortality associated with CDx remained after adjusting for factors included in the modified LCPI (adjusted HR [95% CI] = 0.78 [0.75-0.82]) as well as a model without actionable mutations (adjusted HR [95% CI] = 0.70 [0.66-0.73]). Conclusions: Among patients with non-squamous aNSCLC, use of CDx was associated with reduced risk of mortality compared with no CDx.

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Orcutt, Xavier, Ronac Mamtani, Arjun Sondhi, Aaron B. Cohen, and Ravi Bharat Parikh. "Evaluating generalizability of practice-changing randomized clinical trials in non-small cell lung cancer using machine learning-based in-silico trials." Journal of Clinical Oncology 41, no. 16_suppl (June 1, 2023): 9130. http://dx.doi.org/10.1200/jco.2023.41.16_suppl.9130.

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9130 Background: Results of randomized clinical trials (RCTs) of anticancer agents are not generalizable to many real-world patients. Advances in machine learning (ML) and increasing availability of curated real-world data offer opportunities to assess generalizability by simulating trials “in-silico”. Our objective was to assess the generalizability of survival outcomes reported in 2 practice-changing phase III trials in first-line (1L) advanced non-small cell lung cancer (aNSCLC). Methods: Our cohort included patients from the nationwide Flatiron Health EHR-derived de-identified database diagnosed with stage IIIB-IV or recurrent aNSCLC between 2011 and 2020. First, we trained and validated supervised ML models (gradient boosted, random forest, support vector machine and penalized Cox) to predict 1-year survival for patients with aNSCLC; the Lung Cancer Prognostic Index (LCPI), a published disease-specific prognostic index, was used as a comparator model. We used 130 demographic, vital sign, laboratory, and biomarker features at aNSCLC diagnosis to build models. Second, we used the best-performing ML model to create 4 prognostic risk groups. Third, we simulated 2 seminal trials for 1L treatment of aNSCLC, using inverse probability of treatment-weighted survival analyses, coarsely reproducing inclusion/exclusion criteria across ML-derived risk groups. We compared median overall survival (mOS) using Kaplan-Meier curves from the start of 1L treatment to death in in-silico trials (ISTs) vs. RCTs. Results: Our cohort included 61,339 patients with aNSCLC. The best-performing gradient boosted model outperformed the LCPI (AUC 0.784 vs 0.688). In ISTs, survival benefits of novel treatments varied across risk groups and were generally lower in ISTs compared to RCTs (Table). IST results in high- and very high-risk patients were inconsistent with RCT survival results. For example, for KEYNOTE-024, mOS in the pembrolizumab arm was 30.0 months, whereas in the IST, mOS among patients receiving pembrolizumab varied from 1.3 months in very high-risk patients to 41.5 months in low-risk patients. RCT results overestimated treatment effects for high-risk aNSCLC patients. Conclusions: ML-based ISTs can reveal heterogeneity in real-world survival outcomes associated with novel oncology treatments and elucidate populations for whom RCT results generalize poorly. [Table: see text]

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Baumgart, Meike, Karin Schubert, Marc Bramkamp, and Julia Frunzke. "Impact of LytR-CpsA-Psr Proteins on Cell Wall Biosynthesis in Corynebacterium glutamicum." Journal of Bacteriology 198, no. 22 (August 22, 2016): 3045–59. http://dx.doi.org/10.1128/jb.00406-16.

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ABSTRACT Proteins of the LCP (LytR, CpsA, Psr) family have been shown to inherit important roles in bacterial cell wall biosynthesis. However, their exact function in the formation of the complex cell wall structures of the Corynebacteriales , including the prominent pathogens Mycobacterium tuberculosis and Corynebacterium diphtheriae , remains unclear. Here, we analyzed the role of the LCP proteins LcpA and LcpB of Corynebacterium glutamicum , both of which localize at regions of nascent cell wall biosynthesis. A strain lacking lcpB did not show any growth-related or morphological phenotype under the tested conditions. In contrast, conditional silencing of the essential lcpA gene resulted in severe growth defects and drastic morphological changes. Compared to the wild-type cell wall, the cell wall of this mutant contained significantly less mycolic acids and a reduced amount of arabinogalactan. In particular, rhamnose, a specific sugar component of the linker that connects arabinogalactan and peptidoglycan, was decreased. Complementation studies of the lcpA -silencing strain with several mutated and truncated LcpA variants suggested that both periplasmic domains are essential for function whereas the cytoplasmic N-terminal part is dispensable. Successful complementation experiments with proteins of M. tuberculosis and C. diphtheriae revealed a conserved function of LCP proteins in these species. Finally, pyrophosphatase activity of LcpA was shown in an in vitro assay. Taken together, our results suggest that LCP proteins are responsible for the transfer of arabinogalactan onto peptidoglycan in actinobacterial species and support a crucial function of a so-far-uncharacterized C-terminal domain (LytR_C domain) which is frequently found at the C terminus of the LCP domain in this prokaryotic phylum. IMPORTANCE About one-third of the world's population is infected with Mycobacterium tuberculosis , and multiple-antibiotic resistance provokes the demand for novel antibiotics. The special cell wall architecture of Corynebacteriales is critical for treatments because it is either a direct target or a barrier that the drug has to cross. Here, we present the analysis of LcpA and LcpB of the closely related Corynebacterium glutamicum , the first of which is an essential protein involved in cell wall biogenesis. Our work provides a comprehensive characterization of the impact of LCP proteins on cell wall biogenesis in this medically and biotechnologically important class of bacteria. Special focus is set on the two periplasmic LcpA domains and their contributions to physiological function.

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Basik, Ann Anni, Jayaram Nanthini, Tiong Chia Yeo, and Kumar Sudesh. "Rubber Degrading Strains: Microtetraspora and Dactylosporangium." Polymers 13, no. 20 (October 13, 2021): 3524. http://dx.doi.org/10.3390/polym13203524.

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Rubber composed of highly unsaturated hydrocarbons, modified through addition of chemicals and vulcanization are widely used to date. However, the usage of rubber, faces many obstacles. These elastomeric materials are difficult to be re-used and recovered, leading to high post-consumer waste and vast environmental problems. Tyres, the major rubber waste source can take up to 80 years to naturally degrade. Experiments show that the latex clearing proteins (Lcp) found in Actinobacteria were reportedly critical for the initial oxidative cleavage of poly(cis-1,4-isoprene), the major polymeric unit of rubber. Although, more than 100 rubber degrading strains have been reported, only 8 Lcp proteins isolated from Nocardia (3), Gordonia (2), Streptomyces (1), Rhodococcus (1), and Solimonas (1) have been purified and biochemically characterized. Previous studies on rubber degrading strains and Lcp enzymes, implied that they are distinct. Following this, we aim to discover additional rubber degrading strains by randomly screening 940 Actinobacterial strains isolated from various locations in Sarawak on natural rubber (NR) latex agar. A total of 18 strains from 5 genera produced clearing zones on NR latex agar, and genes encoding Lcp were identified. We report here lcp genes from Microtetraspora sp. AC03309 (lcp1 and lcp2) and Dactylosporangium sp. AC04546 (lcp1, lcp2, lcp3), together with the predicted genes related to rubber degradation. In silico analysis suggested that Microtetraspora sp. AC03309 is a distinct species closely related to Microtetraspora glauca while Dactylosporangium sp. AC04546 is a species closely related to Dactylosporangium sucinum. Genome-based characterization allowed the establishment of the strains taxonomic position and provided insights into their metabolic potential especially in biodegradation of rubber. Morphological changes and the spectrophotometric detection of aldehyde and keto groups indicated the degradation of the original material in rubber samples incubated with the strains. This confirms the strains’ ability to utilize different rubber materials (fresh latex, NR product and vulcanized rubber) as the sole carbon source. Both strains exhibited different levels of biodegradation ability. Findings on tyre utilization capability by Dactylosporangium sp. AC04546 is of interest. The final aim is to find sustainable rubber treatment methods to treat rubber wastes.

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Basik, Ann Anni, Jayaram Nanthini, Tiong Chia Yeo, and Kumar Sudesh. "Rubber Degrading Strains: Microtetraspora and Dactylosporangium." Polymers 13, no. 20 (October 13, 2021): 3524. http://dx.doi.org/10.3390/polym13203524.

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Rubber composed of highly unsaturated hydrocarbons, modified through addition of chemicals and vulcanization are widely used to date. However, the usage of rubber, faces many obstacles. These elastomeric materials are difficult to be re-used and recovered, leading to high post-consumer waste and vast environmental problems. Tyres, the major rubber waste source can take up to 80 years to naturally degrade. Experiments show that the latex clearing proteins (Lcp) found in Actinobacteria were reportedly critical for the initial oxidative cleavage of poly(cis-1,4-isoprene), the major polymeric unit of rubber. Although, more than 100 rubber degrading strains have been reported, only 8 Lcp proteins isolated from Nocardia (3), Gordonia (2), Streptomyces (1), Rhodococcus (1), and Solimonas (1) have been purified and biochemically characterized. Previous studies on rubber degrading strains and Lcp enzymes, implied that they are distinct. Following this, we aim to discover additional rubber degrading strains by randomly screening 940 Actinobacterial strains isolated from various locations in Sarawak on natural rubber (NR) latex agar. A total of 18 strains from 5 genera produced clearing zones on NR latex agar, and genes encoding Lcp were identified. We report here lcp genes from Microtetraspora sp. AC03309 (lcp1 and lcp2) and Dactylosporangium sp. AC04546 (lcp1, lcp2, lcp3), together with the predicted genes related to rubber degradation. In silico analysis suggested that Microtetraspora sp. AC03309 is a distinct species closely related to Microtetraspora glauca while Dactylosporangium sp. AC04546 is a species closely related to Dactylosporangium sucinum. Genome-based characterization allowed the establishment of the strains taxonomic position and provided insights into their metabolic potential especially in biodegradation of rubber. Morphological changes and the spectrophotometric detection of aldehyde and keto groups indicated the degradation of the original material in rubber samples incubated with the strains. This confirms the strains’ ability to utilize different rubber materials (fresh latex, NR product and vulcanized rubber) as the sole carbon source. Both strains exhibited different levels of biodegradation ability. Findings on tyre utilization capability by Dactylosporangium sp. AC04546 is of interest. The final aim is to find sustainable rubber treatment methods to treat rubber wastes.

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Nakagawa, Ichiro, Masashi Kotsugi, HunSoo Park, Shohei Yokoyama, Takanori Furuta, Kenta Nakase, Ai Okamoto, Kaoru Myouchin, Syuichi Yamada, and Hiroyuki Nakase. "Lipid Core Burden Index Assessed by Near-Infrared Spectroscopy of Symptomatic Carotid Plaques: Association with Magnetic Resonance T1-Weighted Imaging." Cerebrovascular Diseases 50, no. 5 (2021): 597–604. http://dx.doi.org/10.1159/000516888.

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Introduction: Vulnerable plaques are a strong predictor of cerebrovascular ischemic events, and high lipid core plaques (LCPs) are associated with an increased risk of embolic infarcts during carotid artery stenting (CAS). Recent developments in magnetic resonance (MR) plaque imaging have enabled noninvasive assessment of carotid plaque vulnerability, and the lipid component and intraplaque hemorrhage (IPH) are visible as high signal intensity areas on T1-weighted MR images. Recently, catheter-based near-infrared spectroscopy (NIRS) has been shown to accurately distinguish LCPs without IPH. This study aimed to determine whether the results of assessment of high LCPs by catheter-based NIRS correlate with the results of MR plaque imaging. Methods: We recruited 82 consecutive symptomatic carotid artery stenosis patients who were treated with CAS under NIRS and MR plaque assessment. Maximum lipid core burden index (max-LCBI) at minimal luminal areas (MLA), defined as max-LCBIMLA, and max-LCBI for any 4-mm segment in a target lesion, defined as max-LCBIAREA, were assessed by NIRS. Correlations were investigated between max-LCBI and MR T1-weighted plaque signal intensity ratio (T1W-SIR) and MR time-of-flight signal intensity ratio (TOF-SIR) in the same regions as assessed by NIRS. Results: Both T1W-SIRMLA and T1W-SIRAREA were significantly lower in the high LCP group (max-LCBI >504, p < 0.001 for both), while TOF-SIRMLA and TOF-SIRAREA were significantly higher in the high LCP group (p < 0.001 and p = 0.004, respectively). A significant linear correlation was present between max-LCBIMLA and both TIW-SIRMLA and TOF-SIRMLA (r = −0.610 and 0.452, respectively, p < 0.0001 for both). Furthermore, logistic regression analysis revealed that T1W-SIRMLA and TOF-SIRMLA were significantly associated with a high LCP assessed by NIRS (OR, 44.19 and 0.43; 95% CI: 6.55–298.19 and 0.19–0.96; p < 0.001 and = 0.039, respectively). Conclusions: A high LCP assessed by NIRS correlates with the signal intensity ratio of MR imaging in symptomatic patients with unstable carotid plaques.

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Yunitasari, F., L. D. Mahfudz, and I. Mangisah. "Pengaruh Penggunaan Limbah Cair Pemindangan Ikan dalam Ransum terhadap Efisiensi Penggunaan Protein Itik Persilangan Mojosari Peking Fase Pertumbuhan." Jurnal Sain Peternakan Indonesia 13, no. 4 (January 9, 2019): 412–19. http://dx.doi.org/10.31186/jspi.id.13.4.412-419.

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Penelitian ini bertujuan untuk mengkaji pengaruh penggunaan limbah cair pemindangan ikan dalam ransum terhadap efisiensi penggunaan protein itik Mojosari Peking pada fase pertumbuhan. Materi yang digunakan adalah itik Mojosari Peking (MP) jantan berumur 3 minggu sebanyak 120 ekor dengan bobot badan rata-rata 520,30±47,82 g. Bahan pakan yang digunakan antara lain jagung, bekatul, limbah cair pemindangan ikan (LCPI), dan konsentrat CP 144. Penelitian disusun dalam rancangan acak lengkap (RAL) dengan 4 perlakuan dan 5 ulangan. Perlakuan terdiri dari T0 (pakan tanpa penggunaan limbah cair pemindangan ikan), T1 (penggunaan limbah cair pemindangan ikan 2,5%), T2 (penggunaan limbah cair pemindangan ikan 5,0%), dan T3 (penggunaan limbah cair pemindangan ikan 7,5%). Parameter yang diamati meliputi konsumsi protein, massa protein daging (MPD), rasio efisiensi protein (REP) dan income over feed cost (IOFC). Data hasil penelitian dianalisis ragam dengan uji F pada taraf uji 5%, kemudian dilanjutkan dengan uji wilayah ganda Duncan apabila terdapat pengaruh perlakuan yang nyata. Hasil analisis ragam menunjukkan bahwa penggunaan limbah cair pemindangan ikan dalam ransum itik MP tidak berpengaruh nyata (p>0,05) terhadap konsumsi protein, dan REP, tetapi memberikan pengaruh nyata (p<0,05) terhadap MPD. IOFC terbesar diperoleh pada perlakuan T1. Kesimpulan dari penelitian ini adalah penggunaan 2,5% limbah cair pemindangan ikan dapat meningkatkan nilai MPD dan IOFC sehingga pakan akan lebih bernilai ekonomis.Kata Kunci : limbah cair pemindangan ikan, itik persilangan mojosari peking, efisiensi penggunaan protein

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Ļubenko, Jeļena, Viktorija Perepjolkina, Kristīne Mārtinsone, Elmārs Rancāns, and Ainārs Stepens. "THE RELATIONSHIP BETWEEN SUICIDE IDEATION, DEPRESSIVE SYMPTOMS AND PERSONALITY TRAITS IN ELDERLY: PILOT STUDY RESULTS." SOCIETY. INTEGRATION. EDUCATION. Proceedings of the International Scientific Conference 7 (May 25, 2018): 129–39. http://dx.doi.org/10.17770/sie2018vol1.3273.

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Healthy aging and the research on the related factors is one of the World Health Organization's priorities for the 2020-2030 years. Depression and suicidal ideation can adversely affect an individual's aging experience. Personality traits are important factors that allow to predict the individual's behavior in different situations, as well as the accurance of emotional difficulties. The aim of the study was to investigate the relationship between symptoms of depression, suicidal ideation and personality traits in elderly, and to determine whether the relationship between personality traits and suicidal ideation remains statistically significant after controlling the severity of depression symptoms. Thirty nine respondents aged 65 and over were included in the pilot study (M = 71.23; SD = 4.95). The Latvian Clinical Personality Inventory (LCPI) was used for data collection. The results indicate a statistically significant relationship between several personality traits, depression symptoms and suicidal ideation for elderly. Controlling the severity of depression symptoms, statistically significant correlations were found between suicidal ideation and personality traits such as distrust, social withdrawal, self-harm, dissociation proneness on facet level and introversion on domain level. The results of the pilot study largely coincide with the results of previous studies and indicate that certain personality traits, irrespective of the degree of severity of depression symptoms, can explain an additional variation in suicidal thoughts and, hence, the risk of suicide for elderly. It would be important to conduct a study with similar variables in a larger sample.

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Levina, J., V. Perejolkina, K. Martinsone, S. Mihailova, and J. Kolesnikova. "The relationship between anomia and maladaptive personality traits." SHS Web of Conferences 40 (2018): 02006. http://dx.doi.org/10.1051/shsconf/20184002006.

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Research of anomia is topical in the period when a society endures social and economic changes. On the other hand, the modern Western society is characterized by a rapid speed of development, by diversity, variability, multicultural life. In such conditions for some individuals it could be difficult to make life choices. Such individuals can get into “an anomic state”. In a situation of rapid social and political changes as well as simply in the modern diverse and rapidly developing society pathological personality traits can serve as a factor that aggravates anomic feelings. Thus, the main purpose of this research is to investigate relations between anomia and pathological personality traits. The sample consisted of 83 Latvian inhabitants aged from 19 to 47 years (16.9 % males, 83.1 % females). The Anomia Questionnaire – AQ [1] and the Latvian Clinical Personality Inventory – LCPI v2.2 [2] were used. It is found that there are multiple relations between all six sub-dimensions of anomia and certain different maladaptive personality traits. Irresponsibility is the only personality trait that predicts deviation from prescribed rules or customs. Negative emotionality is the only significant predictor for social distrust. Suspiciousness that falls within a first-order domain Psychoticism and a second-order domain Schizotypy is the most significant predictor of estrangement to others as a dimension of anomia. Eccentricity that falls within a first-order domain Psychoticism and a second-order domain Schizotypy is the most significant predictor of cultural isolation. Separation insecurity that falls within a first-order domain Dependence and a second-order domain Neuroticism is the most significant predictor of a lack of goal clarity as a dimension of anomia. Depression as a pathological personality trait that falls within a first-order domain Negative emotionality and a second-order domain Neuroticism is the most significant predictor of a lack of goal clarity as a dimension of anomia.

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Huo, Yishan, Kainan Zhang, Songtao Han, Yangchun Feng, and Yongxing Bao. "Lymphocyte cytosolic protein 2 is a novel prognostic marker in lung adenocarcinoma." Journal of International Medical Research 49, no. 11 (November 2021): 030006052110596. http://dx.doi.org/10.1177/03000605211059681.

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Objective Lymphocyte cytosolic protein 2 (LCP2) is often ectopically expressed in various human tumors. However, the clinical significance and role of LCP2 in lung adenocarcinoma (LUAD) remain unclear. This study explored the prognostic significance of LCP2 in LUAD patients. Methods LCP2 expression in LUAD tissues was analyzed using data from The Cancer Genome Atlas and Genotype-Tissue Expression databases. Western blotting was employed to detect LCP2 expression in LUAD. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to explore signaling pathways mediated by LCP2 co-regulatory genes. Immunohistochemistry was used to examine levels of LCP2 and programmed death ligand 1 (PD-L1) in 68 LUAD patients. Associations between LCP2 expression and clinicopathological features, prognoses, and PD-L1 levels among the LUAD in-patients were analyzed. Results Among the 68 LUAD in-patients, LCP2 expression was correlated with clinical stage and lymph node metastasis. LUAD patients with high LCP2 expression were associated with increased overall survival. LCP2 expression may be associated with an enrichment of several immune functions. Moreover, our immunohistochemistry results demonstrated that LCP2 expression was positively correlated with PD-L1 expression in LUAD tissues. Conclusions In the study, LCP2 was found to be a favorable prognostic biomarker in LUAD patients.

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Metzger, Kristen E., Yvonne Rucker, Mary Callaghan, Michelle Churchill, Borko D. Jovanovic, Teresa R. Zembower, and Maureen K. Bolon. "The Burden of Mucosal Barrier Injury Laboratory-Confirmed Bloodstream Infection among Hematology, Oncology, and Stem Cell Transplant Patients." Infection Control & Hospital Epidemiology 36, no. 2 (December 18, 2014): 119–24. http://dx.doi.org/10.1017/ice.2014.38.

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ObjectiveTo evaluate the impact and burden of the new National Healthcare Safety Network surveillance definition, mucosal barrier injury laboratory-confirmed bloodstream infection (MBI-LCBI), in hematology, oncology, and stem cell transplant populations.DesignRetrospective cohort study.SettingTwo hematology, oncology, and stem cell transplant units at a large academic medical center.MethodsCentral line–associated bloodstream infections (CLABSIs) identified during a 14-month period were reviewed and classified as MBI-LCBI or non-MBI-LCBI (MBI-LCBI criteria not met). During this period, interventions to improve central line maintenance were implemented. Characteristics of patients with MBI-LCBI and non-MBI-LCBI were compared. Total CLABSI, MBI-LCBI, and non-MBI-LCBI rates were compared between baseline and postintervention phases of the study period.ResultsAmong 66 total CLABSI cases, 47 (71%) met MBI-LCBI criteria. Patients with MBI-LCBI and non-MBI-LCBI were similar in regard to most clinical and demographic characteristics. Between the baseline and postintervention study periods, the overall CLABSI rate decreased from 3.37 to 3.21 infections per 1,000 line-days (incidence rate ratio, 0.95; 4.7% reduction, P=.84), the MBI-LCBI rate increased from 2.08 to 2.61 infections per 1,000 line-days (incidence rate ratio, 1.25; 25.3% increase, P=.44), and the non-MBI-LCBI rate decreased from 1.29 to 0.60 infections per 1,000 line-days (incidence rate ratio, 0.47; 53.3% reduction, P=.12).ConclusionsMost CLABSIs identified among hematology, oncology, and stem cell transplant patients met MBI-LCBI criteria, and CLABSI prevention efforts did not reduce these infections. Further review of the MBI-LCBI definition and impact is necessary to direct future definition changes and reporting mandates.Infect Control Hosp Epidemiol 2014;00(0): 1–6

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Pereira, Fabiano Vargas, Aloir Antonio Merlo, Françoise Bley, Isabelle Morfin, Olga Maria Ritter, Nádya Pesce da Silveira, and Françoise Ehrburger‐Dolle. "Smectic ordering in side‐chain liquid crystalline polymers (LCPs) and in LCP–silica nanocomposites." Liquid Crystals 35, no. 3 (March 2008): 299–313. http://dx.doi.org/10.1080/02678290701858338.

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Kong, Qinghong, Lan Li, Manman Zhang, Huiyu Chai, Weixi Li, Fang Zhu, and Junhao Zhang. "Improving the Thermal Stability and Flame Retardancy of Epoxy Resins by Lamellar Cobalt Potassium Pyrophosphate." Polymers 14, no. 22 (November 15, 2022): 4927. http://dx.doi.org/10.3390/polym14224927.

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In order to improve the fire retardancy of epoxy resin (EP), lamellar cobalt potassium pyrophosphate (LCPP) nanocrystal whiskers with a length of 100–300 nm were designed and synthesized by a liquid technique. LCPP with high thermal stability was blended into EP to prepare the EP/LCPP composites. The results show that the EP/LCPP composites have higher thermal stability and produce more residues compared to pure EP. The combustion results display that the LOI value of the EP/10wt%LCPP composites was significantly improved to 35.9%, and the EP/6wt%LCPP composite can reach a UL-94 V-1 rating. Additionally, the peak heat release rate and peak smoke production rate of the EP/10wt%LCPP composites dramatically decreased by 43.8% and 48.5%, respectively. The improved flame retardancy and smoke suppression are mainly attributed to the inherent physical barrier of LCPP and the excellent catalytic carbonization ability of LCPP.

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Mahat, Upendra, Bhavuk Garg, Hrishikesh M. Mehta, Heesun J. Rogers, Andrei Ivanov, Chao-Yie Yang, Aron Flagg, and Seth J. Corey. "Lymphocyte Cytosolic Protein 1 I232F Mutation Impairs Granulocytic Proliferation with a G2/M Block in Severe Neutropenia." Blood 138, Supplement 1 (November 5, 2021): 434. http://dx.doi.org/10.1182/blood-2021-152781.

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Abstract Introduction: As critical effectors of innate immune response, neutrophils migrate from the vasculature into inflamed tissue, where they engage in phagocytosis and clearance of pathogens and apoptotic cells. Migration, phagocytosis, and granule release require multiple well-coordinated events involving remodeling of the actin cytoskeleton. While mutations of some regulators of actin polymerization, such as Wiskott-Aldrich Syndrome gene (WAS) result in Severe Congenital Neutropenia (SCN), the role of actin cytoskeleton in regulating neutrophil ontogeny and functions remain poorly understood. Lymphocyte Cytosolic Protein 1 (LCP1) is an actin bundling protein encoded by LCP1 gene. A 7-year-old girl presented at age of 2 years with chronic severe neutropenia and frequent infections. Next generation sequencing did not reveal a variant in genes known to cause neutropenia. Whole exome sequencing identified a novel LCP1 p.I232F variant of unknown significance, which was not present in either parent. Bone marrow examination revealed a granulocytic hypoplasia with significant dysmorphic giant granulocytes with complex hypersegmentation and increased apoptosis. (Figure 1A) Based on these observations, we hypothesized that missense mutation in LCP1 (I232F) impairs granulocytic proliferation, and causes neutropenia. Aims: To investigate the role of LCP1 I232F, discovered in a child with severe symptomatic neutropenia, in granulopoiesis. Methods: in vitro experiments involving the expression of wild type and mutant LCP1 I232F in murine myeloblast cell (32D cells) and human cervical cancer HeLa cell. We deployed various biologic and functional studies to investigate the role of LCP1 I232F in granulocyte proliferation, differentiation, survival, and function. Results: To determine the pathophysiologic significance of LCP1 I232F, we stably transduced interleukin 3-dependent murine myeloblast 32D and human cervical cancer HeLa cell lines with a doxycycline-inducible lentiviral constructs containing the cDNA for either wild type LCP1 or LCP1 I232F. The mutant LCP1 I232F expressing 32D cells showed impaired proliferation with the appearance of dysplastic granulocytic cells. (Figure 1A, B) However, we did not observe block in differentiation. A cell cycle arrest at G2/M phase occurred only in the LCP1 I232F expressing cells (Figure 1B) and was associated with an upregulation of markers of cell cycle arrest (Cdkn1a and Tp53). LCP1 I232F overexpression did not lead to increased expression of genes involved in apoptosis or the unfolded protein response. The LCP1 I232F cells with dysplastic features were further analyzed by confocal microscopy, which demonstrated increased level of F-actin and enrichment of F-actin and LCP1 at the cell cortex. Flow cytometric evaluation of these cells further confirmed the presence of increased level of F actin. (Figure 1C) Expression of LCP1 I232F impaired cell motility and invasiveness in both 32D and HeLa cells. However, mutant LCP1 expressing 32D cells demonstrated normal oxidative burst upon treatment with N-formyl-Met-Leu-Phe (fMLP) and phorbol myristate acetate (PMA). Confocal imaging and subcellular fractionation revealed diffuse localization of LCP1, but only the mutant form was found in the nucleus. (Figure 1C) In LCP1, two tandem CH domains (CH1-CH2 and CH3-CH4) formed two actin-binding domains (ABD1 and ABD2) that bind to actin to participate in actin bundling. I232F is located at LCP1-CH1 that interacts with actin. To study how the mutation impacts LCP1-actin binding, we constructed a model of LCP1-ABD1 based on the cryo-EM structure of F-actin/LCP1-ABD2 and performed 40 ns of molecular dynamics (MD) simulations of LCP1-ABD1 and LCP1-ABD1 I232F alone to study their dynamical motions. We found that LCP1-ABD1 I232F adopted a more stable open conformation than LCP1-ABD1 to bind effectively with F-actin. The model implies a prolonged association of LCP1 I232F with actin than wild-type LCP1, and the consequence is the increased stabilization of actin bundles. Conclusions: Like the WAS gain-of-function mutations in SCN, LCP1 I232F resulted in severe neutropenia with dysplastic granulocytic precursors, cell cycle arrest, impaired motility, and increased F actin. We also found decreased proliferation of granulocytic progenitor and precursor cells. Our findings suggest that LCP1 is an important gene involved in granulopoiesis. Figure 1 Figure 1. Disclosures No relevant conflicts of interest to declare.

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Wang, Man, Huijuan Zhang, Xiaopei Yang, Wei Li, Tianfang Li, and Shengyun Liu. "Laboratory-confirmed bloodstream infection in systemic lupus erythematosus: Risk profiling and short-term mortality." Lupus 29, no. 12 (August 24, 2020): 1520–27. http://dx.doi.org/10.1177/0961203320948964.

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Objectives To delineate laboratory-confirmed bloodstream infection (LCBI), analyze risk factors for its occurrence and predictors for its short-term mortality in systemic lupus erythematosus (SLE) patients. Methods A single center, retrospective, case-controlled study was performed in 159 SLE patients (2013–2019) to identify risk factors of LCBI by comparing patients with LCBI (n = 39) to those without infection (n = 120). The predictors associated with 30-day mortality in LCBI patients were also analyzed. Results Altogether 40 bacteria strains were isolated in 39 LCBI patients with a predominance of the gram-negative bacilli (24 strains, 60.0%). Escherichia coli and Staphylococcus aureus were the leading Gram-negative and Gram-positive microorganisms, respectively. Occurrence of LCBI was independently predicted by: SLE disease duration >4 years, SLEDAI score >4 points, glucocorticoids dose >7.5 mg/d and the previous or concomitant occurrence of autoimmune hemolytic anemia (AIHA) or thrombotic microangiopathy (TMA). Based on the identified risk factors, we developed a matrix model for the risk of future LCBI. The 30-day mortality (39 cases) was 23.1% and healthcare-associated LCBI was a predictor for 30-day mortality in SLE patients compared with community-acquired LCBI. Conclusion Longer duration, higher disease activity and glucocorticoids dose, and occurrence of AIHA or TMA were risk factors of LCBI in SLE and its poor short-term prognosis may attribute to healthcare-associated LCBI.

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Lu, Shaorong, Jianfeng Ban, and Kuo Liu. "Preparation and Characterization of Liquid Crystalline Polyurethane/Al2O3/Epoxy Resin Composites for Electronic Packaging." International Journal of Polymer Science 2012 (2012): 1–4. http://dx.doi.org/10.1155/2012/728235.

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Liquid crystalline polyurethane (LCPU)/Al2O3/epoxy resin composites were prepared by using LCPU as modifier. The mechanical properties, thermal stability, and electrical properties of the LCPU/Al2O3/epoxy resin composites were investigated systematically. The thermal oxidation analysis indicated that LCPU/Al2O3/epoxy resin composites can sustain higher thermal decomposition temperature. Meanwhile, coefficient of thermal expansion (CTE) was also found to decrease with addition of LCPU and nano-Al2O3.

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Tsai, Chen-Liang, Chih-Ying Changchien, Ying Chen, Chine-Rui Lai, Tzu-Min Chen, Hsin-Han Chang, Wen-Chiuan Tsai, et al. "Survival Benefit of Statin with Anti-Angiogenesis Efficacy in Lung Cancer-Associated Pleural Fluid through FXR Modulation." Cancers 14, no. 11 (June 2, 2022): 2765. http://dx.doi.org/10.3390/cancers14112765.

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Lung cancer-related pleural fluid (LCPF) presents as a common complication with limited treatment. Beyond its function in lipid digestion, bile acid was identified as a potent carcinogen to stimulate tumor proliferation. Previous research indicated a correlation between serum bile acid levels and the risk of developing several gastrointestinal cancers. Our study identified elevated bile acid levels in LCPF and increased farnesoid X receptor (FXR) expression as bile acid nuclear receptors in pleural microvessels of lung adenocarcinoma. Additionally, LCPF stimulated the expression of proteins involved in bile acid synthesis and cholesterol metabolism in HUVECs including CYP7A1, StAR, HMGCR, and SREBP2. LCPF-induced endothelial motility and angiogenesis were counteracted by using β-muricholic acid as an FXR antagonist. Moreover, we investigated the efficacy of cholesterol-lowering medications, such as cholestyramine, fenofibrate, and atorvastatin, in regulating LCPF-regulated angiogenesis. Along with suppressing endothelial proliferation and angiogenesis, atorvastatin treatment reversed cholesterol accumulation and endothelial junction disruption caused by LCPF. Statin treatment inhibited LCPF-induced endothelial FXR expression as well as the downstream proteins RXR and SHP. Based on the positive findings of suppressing endothelial angiogenesis, our group further incorporated the effect of statin on clinical patients complicated with LCPF. A Kaplan–Meier analysis revealed the clinical benefit of statin exposure in patients with lung adenocarcinoma with LCPF. Conclusively, our study demonstrated the ability of statin to alleviate LCPF-induced angiogenesis in patients with LCPF via FXR modulation.

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Liszewski Zilla, Megan, J. Mark Lunderberg, Olaf Schneewind, and Dominique Missiakas. "Bacillus anthracis lcpGenes Support Vegetative Growth, Envelope Assembly, and Spore Formation." Journal of Bacteriology 197, no. 23 (September 21, 2015): 3731–41. http://dx.doi.org/10.1128/jb.00656-15.

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ABSTRACTBacillus anthracis, a spore-forming pathogen, replicates as chains of vegetative cells by regulating the separation of septal peptidoglycan. Surface (S)-layer proteins andB. anthracisS-layer-associated proteins (BSLs) function as chain length determinants and are assembled in the envelope by binding to the secondary cell wall polysaccharide (SCWP).B. anthracisexpresses six different genes encoding LytR-CpsA-Psr (LCP) enzymes (lcpB1to -4,lcpC, andlcpD), which when expressed inStaphylococcus aureuspromote attachment of wall teichoic acid to peptidoglycan. Mutations inB. anthracislcpB3andlcpDcause aberrations in cell size and chain length that can be explained as discrete defects in SCWP assembly; however, the function of the otherlcpgenes is not known. By deleting combinations oflcpgenes from theB. anthracisgenome, we generated variants with singlelcpgenes.B. anthracisexpressinglcpB3alone displayed physiological cell size, vegetative growth, spore formation, and S-layer assembly. Strains expressinglcpB1orlcpB4displayed defects in cell size and shape, S-layer assembly, and spore formation yet sustained vegetative growth. In contrast, thelcpB2strain was unable to grow unless the gene was expressed from a multicopy plasmid (lcpB2++), and variants expressinglcpCorlcpDdisplayed severe defects in growth and cell shape. ThelcpB2++,lcpC, orlcpDstrains supported neither S-layer assembly nor spore formation. We propose a model whereby LCP enzymes fulfill partially overlapping functions in transferring SCWP molecules to discrete sites within the bacterial envelope.IMPORTANCEProducts of genes essential for bacterial envelope assembly represent targets for antibiotic development. The LytR-CpsA-Psr (LCP) enzymes tether bactoprenol-linked intermediates of secondary cell wall polymers to the C6 hydroxyl ofN-acetylmuramic acid in peptidoglycan; however, the role of LCPs as a target for antibiotic therapy is not defined. We show here that LCP enzymes are essential for the cell cycle, vegetative growth, and spore formation ofBacillus anthracis, the causative agent of anthrax disease. Furthermore, we assign functions for each of the six LCP enzymes, including cell size and shape, vegetative growth and sporulation, and S-layer and S-layer-associated protein assembly.

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31

da Silva, Ludmila Bezerra, Lidia dos Santos Miragaia, Leandro Carvalho Dantas Breda, Cecilia Mari Abe, Mariana Costa Braga Schmidt, Ana Maria Moro, Denize Monaris, et al. "Pathogenic Leptospira Species Acquire Factor H and Vitronectin via the Surface Protein LcpA." Infection and Immunity 83, no. 3 (December 22, 2014): 888–97. http://dx.doi.org/10.1128/iai.02844-14.

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Upon infection, pathogenicLeptospiraspecies bind several complement regulators in order to overcome host innate immunity. We previously characterized a 20-kDa leptospiral surface protein which interacts with C4b binding protein (C4BP):leptospiralcomplement regulator-acquiringprotein A (LcpA). Here we show that LcpA also interacts with human factor H (FH), which remains functionally active once bound to the protein. Antibodies directed against short consensus repeat 20 (SCR20) inhibited binding of FH to LcpA by approximately 90%, thus confirming that this particular domain is involved in the interaction. We have also shown for the first time that leptospires bind human vitronectin and that the interaction is mediated by LcpA. Coincubation with heparin blocked LcpA-vitronectin interaction in a dose-dependent manner, strongly suggesting that binding may occur through the heparin binding domains of vitronectin. LcpA also bound to the terminal pathway component C9 and inhibited Zn2+-induced polymerization and membrane attack complex (MAC) formation. Competitive binding assays indicated that LcpA interacts with C4BP, FH, and vitronectin through distinct sites. Taken together, our findings indicate that LcpA may play a role in leptospiral immune evasion.

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32

Bayır, Mehtap, and Gökhan Arslan. "Bioinformatics of Nile tilapia (Oreochromis niloticus) lymphocyte cytosolic protein 1 (lcp1) gene." Ege Journal of Fisheries and Aquatic Sciences 38, no. 1 (March 15, 2021): 63–68. http://dx.doi.org/10.12714/egejfas.38.1.07.

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Bioinformatics analysis of lymphocyte cytosolic protein 1 (lcp1) gene in tilapia (Oreochromis niloticus) which is a model organism in experimental studies were completed in this study. For this purpose, characterization and identification of lcp1 has been completed and ensembl database has been used to design the structure of lcp1 gene. In addition, the chromosome region of tilapia lcp1 and other genes in the same region with lcp1were determined. The chromosome of these genes were detected in zebrafish and human which are identical orthologos of tilapia. Conserved gene synteny designed manually according to these chromosomal regions. In addition, amino acid sequences synthesized by lcp1 gene in some vertebrates were determined using some bioinformatics databases such as UNIPROT, ENSEMBL and NCBI before determine the phylogenetic relationship between these organisms and tilapia. Sequence similarity-identity rate of tilapia lcp1 gene with zebrafish, rainbow trout, human, mouse and platyfish lcp1/Lcp1 was calculated using BLOSUM62 matrix algorithm. This study is of great importance for the completion of in silico analysis of lcp1 gene in tilapia because it is an aquatic model organism and it has an important place among economic aquaculture species. However this study provides the basic pioneering information for the future studies on molecular stress response in fish.

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33

Gao, Xing Jian, Qi Zhang, Dong Bin Wei, Si Hai Jiao, and Zheng Yi Jiang. "Dry Sliding Wear of As-Cast and Thermomechanically Processed Low Chromium White Cast Iron." Advanced Materials Research 797 (September 2013): 725–30. http://dx.doi.org/10.4028/www.scientific.net/amr.797.725.

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This investigation attempts to improve the wear resistance of low chromium white cast iron (LCCI) by thermomechanical treatment. The thermomechanical treatment of the brittle LCCI with crack-free was successfully carried out by bonding it with a ductile low carbon steel firstly. Afterwards the dry sliding wear behavior of as-cast (LCCI-A) and thermomechanically processed (LCCI-B) samples was studied using a pin-on-disc apparatus under different test conditions. The microstructural examination shows that the refined supercooled austenite and plenty of secondary carbides in LCCI-B replaced the original microstructure of martensite and retained austenite with network carbide in LCCI-A. This significant evolution is beneficial to form and stabilise the oxide layer on the substrate, which makes the oxidational wear rather than abrasive wear or delamination dominating the wear process so that the improvement of the wear resistance of LCCI was achieved by hot working.

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de Araújo, Patricia Rocha, Giovana Maria Fioramonti Calixto, Victor Hugo Sousa Araújo, Mariana Rillo Sato, Camila Fernanda Rodero, João Augusto Oshiro-Junior, Taís Maria Bauab, and Marlus Chorilli. "In vivo study of hypericin-loaded poloxamer-based mucoadhesive in situ gelling liquid crystalline precursor system in a mice model of vulvovaginal candidiasis." Medical Mycology 59, no. 8 (February 24, 2021): 821–27. http://dx.doi.org/10.1093/mmy/myab006.

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Abstract The present study reports the performance of the pigment hypericin (HYP)-loaded poloxamer-based mucoadhesive in situ gelling liquid crystalline precursor system (LCPS) for the treatment of vulvovaginal candidiasis (VVC) in mice. LCPS composed of 40% of ethoxylated and propoxylated cetyl alcohol, 30% of oleic acid and cholesterol (7:1), 30% of a dispersion of 16% poloxamer 407 and 0.05% of HYP (HYP-LCPS) was prepared and characterized by polarized light microscopy (PLM), small-angle X-ray scattering (SAXS) and ex vivo permeation and retention studies across vaginal porcine mucosa were performed. In addition, the antifungal properties of the HYP-LCPS were evaluated in a murine in vivo model; for this, infected C57BL female mice groups were treated with both HYP in solution and HYP-LCPS, and after 6 days colony forming unit (CFU)/ml count was performed. PLM and SAXS confirmed that HYP-LCPS is a microemulsion situated in boundary transition region confirming its action as an LCPS. When in contact with simulated vaginal fluid, HYP-LCPS became rigid and exhibited maltase crosses and bragg peaks characteristics of lamellar phase. Ex vivo permeation and retention studies showed that HYP-LCPS provides a localized treatment on the superficial layers of porcine vaginal mucosa. HYP-LCPS induced a significant reduction in the number of CFU/ml in the mice; thus this formulation indicated it is as effective as a commercial dosage form. It was concluded that LCPS maintains the biological activity of HYP and provides an adequate drug delivery system for this lipophilic molecule at the vaginal mucosa, being a promising option in cases of VVC.

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Dandoy, Christopher E., Min Chen, Soyoung Kim, Krishna Komanduri, Caroline A. Lindemans, Miguel-Angel Perales, and Marcie L. Riches. "Burden and Outcomes of Mucosal Barrier Injury-Laboratory Confirmed Bloodstream Infections (MBI-LCBI) in the First 100 Days after Allogeneic Stem Cell Transplant: A CIBMTR Analysis." Blood 132, Supplement 1 (November 29, 2018): 3375. http://dx.doi.org/10.1182/blood-2018-99-114629.

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Abstract Background: Patients undergoing stem cell transplant (SCT) are at risk of bloodstream infections (BSI). BSI led to prolonged hospitalization, intensive care admissions, prolonged antibiotic treatment and increased mortality. Recently, the Centers for Disease Control and Prevention developed a modification of the Central line associated bloodstream infection (CLABSI) definition, termed "mucosal barrier injury laboratory-confirmed bloodstream infection" (MBI-LCBI) to differentiate BSI likely related to mucosal barrier injury. BSI are identified as an MBI-LCBI if: (1) it resulted from 1 or more of a group of selected organisms known to be commensals of the oral cavity or gastrointestinal tract and (2) occurred in a patient with signs or symptoms compatible with the presence of mucosal barrier injury such as gastrointestinal graft-versus-host disease and/or neutropenia. We utilized the CIBMTR database to determine the incidence and timing of MBI-LCBI, risk factors for development of MBI-LCBI, and compare transplant outcomes by 1 year after SCT. Methods: We identified 16,875 pediatric and adult patients receiving first allogeneic transplant from 2009-2016. Patients were classified into 4 categories based on the occurrence of BSI in first 100 days: MBI-LCBI (n=1434; 8.5%), MBI-LCBI +other BSI (n=700; 4.1%), BSI only (n=3016; 17.8%), and control (n=11725; 69.5%) (Figure 1). Demographics and outcomes, including overall survival (OS), chronic GVHD, and transplant-related mortality (TRM, for malignant disease patients only), were compared between groups. Results: The cumulative incidence of MBI-LCBI was 13% (99% CI: 12-13%) by day 100 whereas the probability for another BSI not meeting MBI-LCBI criteria was 22% (99% CI: 21-23%) by day 100. The median time from transplant to first MBI-LCBI was 8 days (<1-98), MBI-LCBI + other BSI 10 days (<1-99), and other BSI was 38 days (<1-100). Karnofsky/Lansky performance status <90 [RR 1.21 (99% CI: 1.06 - 1.38)], myeloablative conditioning [RR 1.45 (99% CI: 1.27-1.69)], post-transplant cyclophosphamide as GVHD prophylaxis [RR 1.83 (99% CI: 1.40 - 2.39)], and receipt of cord blood [RR 2.89 (99% CI: 2.06 - 4.06)] were associated with a significant increase in the risk of MBI-LCBI (Table 1). The 1 year OS was inferior for patients with MBI-LCBI only [59% (99% CI: 56 - 61%); RR 1.86 (99% CI: 1.65 - 2.09)], Other BSI only [60% (99% CI: 58 - 63%); RR 1.86 (99% CI: 1.70 - 2.04)], and MBI-LCBI + Other BSI [46% (99% CI: 41 - 50%); RR 2.79 (99% CI: 2.42 - 3.23)] compared to controls [72% (71 - 73); p <0.0001] (Table 2). There was no impact of MBI-LCBI only, Other BSI only, or MBI-LCBI + Other BSI compared to controls on development of chronic GVHD. As expected 1 year TRM in patients with malignant disease was increased with any BSI but was similar for patients with MBI-LCBI only [30% (99% CI: 27 - 34%); RR 2.41 (99% CI: 2.05-2.82] or Other BSI [25% (99% CI: 23 - 27%); RR 2.20 (1.94 - 2.51)] and further worsened for patients with both MBI-LCBI + Other BSI [45% (99% CI: 39 - 50%); RR 4.23 (3.53 - 5.07)] compared to controls [16% (99% CI:15 - 17%)]. Finally, infection as a cause of mortality was higher in patients with MBI-LCBI (19%), BSI only (17%), and MBI-LCBI + BSI (21%) then controls (12%). Discussion: Our data demonstrate approximately 13% of all patients develop at least one MBI-LCBI and an additional 22% of patients develop another BSI in the first 100 days post SCT. Multivariable analysis revealed increased risk of MBI-LCBI with poor performance status, cord blood grafts, myeloablative conditioning, and post-transplant cyclophosphamide GVHD prophylaxis. BSI, whether or not MBI-LCBI, significantly decreases overall survival, primarily related to an increased TRM. The combination of MBI-LCBI and other BSI worsens both TRM and OS, but the respective impact of MBI-LCBI only was similar to Other BSI only. BSI, both MBI-LCBIs and other BSI, lead to significant morbidity and mortality and healthcare resource utilization. Reduction in frequency of BSI should be a major public health and scientific priority. Disclosures Perales: Novartis: Other: Personal fees; Incyte: Membership on an entity's Board of Directors or advisory committees, Other: Personal fees and Clinical trial support; Abbvie: Other: Personal fees; Takeda: Other: Personal fees; Merck: Other: Personal fees.

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Gerber, J. D., M. A. Benecke, F. J. Vorster, and E. E. Van Dyk. "Thermal modelling of low concentrator photovoltaic systems." Journal of Energy in Southern Africa 24, no. 1 (February 1, 2013): 51–55. http://dx.doi.org/10.17159/2413-3051/2013/v24i1a3007.

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Efficient thermal management of low concentrator photovoltaic (LCPV) systems will allow maximizing of the power output and may also substantially prolong operating lifetime. For this reason, it is necessary to develop a thorough understanding of the thermal transfer and dissipation mechanisms associated with an LCPV system. The LCPV system under consideration uses a 7-facet reflector optical design, providing a geometric concentration ratio of approximately 4.85. The LCPV system succeeded in increasing the short circuit current from 1A to 5.6A, demonstrating an effective concentration ratio of approximately 4.75. LCPV system temperatures in excess of 80°C were recorded without a thermal management system. A basic thermal model was developed and assessed under various environmental conditions. The effectiveness of a heat-sink, which reduced the temperature difference between the LCPV receiver temperature and the ambient temperature by 37.5%, was also evaluated. The results discussed in this paper will assist the future development of techniques aimed at reducing the high temperatures associated with LCPV systems.

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Sebastián, David, Laura Herrero, Dolors Serra, Guillermina Asins, and Fausto G. Hegardt. "CPT I overexpression protects L6E9 muscle cells from fatty acid-induced insulin resistance." American Journal of Physiology-Endocrinology and Metabolism 292, no. 3 (March 2007): E677—E686. http://dx.doi.org/10.1152/ajpendo.00360.2006.

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Oversupply of lipids to skeletal muscle causes insulin resistance by promoting the accumulation of lipid-derived metabolites that inhibit insulin signaling. In this study, we tested the hypothesis that overexpression of carnitine palmitoyltransferase I (CPT I) could protect myotubes from fatty acid-induced insulin resistance by reducing lipid accumulation in the muscle cell. Incubation of L6E9 myotubes with palmitate caused accumulation of triglycerides, diacylgycerol, and ceramide, produced an activation of PKCθ and PKCζ, and blocked insulin-stimulated glucose metabolism, reducing insulin-stimulated PKB activity by 60%. Transduction of L6E9 myotubes with adenoviruses encoding for liver CPT I (LCPT I) wild-type (WT), or a mutant form of LCPT I (LCPT I M593S), which is insensitive to malonyl-CoA, produced a twofold increase in palmitate oxidation when LCPT I activity was increased threefold. LCPT I WT and LCPT I M593S-overexpressing L6E9 myotubes showed normal insulin-stimulated glucose metabolism and an improvement in PKB activity when pretreated with palmitate. Moreover, LCPT I WT- and LCPT I M593S-transduced L6E9 myotubes were protected against the palmitate-induced accumulation of diacylglycerol and ceramide and PKCθ and -ζ activation. These results suggest that LCPT I overexpression protects L6E9 myotubes from fatty acid-induced insulin resistance by inhibiting both the accumulation of lipid metabolites and the activation of PKCθ and PKCζ.

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Glyde, Mark, Robert Day, Giselle Hosgood, and Tim Pearson. "The effect of intramedullary pin size and plate working length on plate strain in locking compression plate-rod constructs under axial load." Veterinary and Comparative Orthopaedics and Traumatology 29, no. 06 (November 2016): 451–58. http://dx.doi.org/10.3415/vcot-16-01-0008.

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SummaryObjective: To investigate the effect of intramedullary pin size and plate working length on plate strain in locking compression plate-rod constructs.Methods: A synthetic bone model with a 40 mm fracture gap was used. Locking compression plates with monocortical locking screws were tested with no pin (LCP-Mono) and intramedullary pins of 20% (LCPR-20), 30% (LCPR-30) and 40% (LCPR-40) of intramedullary diameter. Two screws per fragment modelled a long (8-hole) and short (4-hole) plate working length. Strain responses to axial compression were recorded at six regions of the plate via three-dimensional digital image correlation.Results: The addition of a pin of any size provided a significant decrease in plate strain. For the long working length, LCPR-30 and LCPR-40 had significantly lower strain than the LCPR-20, and plate strain was significantly higher adjacent to the screw closest to the fracture site. For the short working length, there was no significant difference in strain across any LCPR constructs or at any region of the plate. Plate strain was significantly lower for the short working length compared to the long working length for the LCP-Mono and LCPR-20 constructs, but not for the LCPR-30 and LCPR-40 constructs.Clinical significance: The increase in plate strain encountered with a long working length can be overcome by the use of a pin of 30–40% intramedullary diameter. Where placement of a large diameter pin is not possible, screws should be placed as close to the fracture gap as possible to minimize plate strain and distribute it more evenly over the plate.

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Ai, Dengxin, Ke Xu, Heng Zhang, Tianheng Chen, and Guilin Wang. "Simulation Research on a Cogeneration System of Low-Concentration Photovoltaic/Thermal Coupled with Air-Source Heat Pump." Energies 15, no. 3 (February 8, 2022): 1238. http://dx.doi.org/10.3390/en15031238.

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In this paper, a low-concentration photovoltaic/thermal (LCPV/T) coupled with air-source heat pump (AHP) system is proposed which fully utilizes the heat generated by LCPV/T and improves the performance of the AHP. The system is built and investigated in the Transient System Simulation Program (TRNSYS) and an experimental room model is established to verify the feasibility of the system. The performance of the system is researched from the perspective of energy and exergy, and the system performance with LCPV/T and without LCPV/T is compared. Finally, the influence of the variation of key parameters of the system is studied. The results indicated that on the coldest day, the electrical efficiency of LCPV/T reached 10% which was equal to the electrical exergy efficiency. The maximum thermal efficiency was 31.88% while thermal exergy efficiency was 2.7%. The maximum coefficient of performance (COP) of AHP was 3.3, and the thermal exergy efficiency was 47%. The indoor temperature was maintained at about 20 °C in the heating season. When LCPV/T was adopted, the COP and thermal exergy efficiency of the AHP was generally higher than those without LCPV/T. In conclusion, the utilization of LCPV/T has a positive impact on the performance of the AHP.

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Xu, Xiaoxu, Zhengzheng Hao, Chao Fan, Qifan Chen, Yanhua Lu, and Yaoqing Wu. "Study on crystallization structure and thermal behavior of iPP induced by a new liquid crystal nucleator." Polymers and Polymer Composites 28, no. 2 (July 24, 2019): 89–99. http://dx.doi.org/10.1177/0967391119864220.

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In this study, a new liquid crystal polymer, named as smectic liquid crystal polymer (LCPs-P), was synthesized by reacting diosgenyl 4-(10-undecen-1-yloyloxy) benzoate with polymethylhydrosiloxane using chloroplatinic acid as a catalyst. The mesomorphic properties of the LCPs-P were characterized with polarizing optical microscopy, differential scanning calorimetry (DSC), thermogravimetric analysis, and X-ray diffraction (XRD). Then, the influence of the LCPs-P as a new nucleator on the crystallization structures, thermal properties, and kinetic properties of isotactic polypropylene (iPP) was investigated by XRD and DSC. The experimental results indicated that the LCPs-P exhibited fan-shaped texture of a smectic A phase between 55°C and 318°C. The content of the β-crystal ( K β) of the iPP blends mainly depended on the LCPs-P content and the crystallization temperature. By optimally combining the LCPs-P content with the crystallization temperature, a high K β could be obtained. When the content of the LCPs-P was 0.9 wt%, the maximum K β could reach 51% after isothermal crystallization for 1 h at 130°C. Moreover, the addition of the LCPs-P could increase the crystallization temperature and shorten the crystallization time of the iPP blends.

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Matuszewska, Anna, Oliwer Sygacz, Łukasz Matuszewski, Szymon Stec, Andrzej Grzegorzewski, and Jacek Gągała. "Association between Polymorphism eNOS4, tPA, Factor V Leiden, Prothrombin, and Methylenetetrahydrofolate Reductase and the Occurrence of Legg–Calvé–Perthes Disease." Journal of Clinical Medicine 12, no. 16 (August 10, 2023): 5209. http://dx.doi.org/10.3390/jcm12165209.

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Background. Legg–Calvé–Perthes (LCPD) disease is a complex condition affecting the femoral head’s epiphysis in children. It occurs with a prevalence ranging from 0.4 to 29.0 cases per 100,000 children under the age of 15. It involves various factors, including genes associated with coagulation and fibrinolysis, pro-inflammatory factors, and vasoactive substances. Methods. We investigated the relationship between genetic mutations associated with coagulation and vascular disorders and the occurrence of LCPD in Polish patients. We performed a study involving 25 patients with LCPD and 100 healthy controls. All subjects were genotyped for eNOS4, Factor V Leiden, prothrombin, tPA25, and MTHFR polymorphism. Results. The analysis revealed that the frequencies of eNOS4 genotypes were significantly different in LCPD patients than in the control group (p = 0.018). The frequencies of 4a allele were significantly higher in patients with LCPD than in the healthy population (26% vs. 9%, p = 0.0012). There were no significant differences in genotype and allele frequencies for Factor V Leiden, prothrombin tPA 25, and MTHFR gene polymorphisms between patients with LCPD and the controls. Conclusions. Genotype and allele frequencies of eNOS4 were significantly higher in patients with LCPD. These findings suggest a potential association between the eNOS gene polymorphism and an increased risk of developing LCPD.

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Pokharel, Rohit Kumar, S. Fujii, R. Shiba, and M. Matsuo. "SIGNIFICANTLY HIGH INCIDENCE OF T-1654C POLYMORPHIC VARIATION IN THE PROTEIN C GENE PROMOTER REGION IN EARLY ONSET LEGG-CALVE' PERTHES DISEASE." Journal of Nepal Medical Association 42, no. 149 (September 1, 2003): 254–9. http://dx.doi.org/10.31729/jnma.618.

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Inherited thrombophilia has been suggested as a cause of micro-thrombosis resulting in femoral head necrosisin Legg-Calve' Perthes Disease (LCPD). Protein C, hepatic zymogens, acts as a thrombotic agent and regulatesthe coagulation cascade. Genotype variations in the promoter region of the protein C gene (T-1654C; A-1641G & A-1476T) have been reported to be associated with plasma protein C levels and thrombotic risk.In order to clarify any association of these polymorphic variations to LCPD, we analyzed the promoterregion of gene for protein C (from –1704 to –1198 nt) in 70 Japanese LCPD cases (8 early-onset & 62 lateonsettype) and 93 controls.Results of direct sequencing of the protein C gene promoter region showed a significantly high incidence ofT-1654C change in early-onset LCPD cases than among the late-onset LCPD and control groups. Ourresults, therefore, suggest a significant association between early onset LCPD and the T-1654C mutation inthe protein C promoter region.Key Words: Protein C gene promoter region polymorphism, micro thrombosis, early-onset LCPD.

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Xie, Zhonghui, Chuanbin Liu, Xu Lu, Zhijie Chen, Nan Zhang, Xinyan Wang, Xiaoqian Li, and Yang Li. "Identification and Verification of Biomarkers and Immune Infiltration in Obesity-Related Atrial Fibrillation." Biology 12, no. 1 (January 12, 2023): 121. http://dx.doi.org/10.3390/biology12010121.

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Obesity is an independent risk factor for atrial fibrillation (AF). However, the mechanisms underlying this crosstalk are still being uncovered. Co-differentially expressed genes (co-DEGs) of AF and obesity microarrays were identified by bioinformatics analysis. Subsequently, functional enrichment, cell-type enrichment, and protein–protein interaction network analyses of co-DEGs were carried out. Then, we validated the hub genes by qRT-PCR of patients’ blood samples. Finally, CIBERSORT was utilized to evaluate the AF microarray to determine immune infiltration and the correlation between validated hub genes and immune cells. A total of 23 co-up-regulated DEGs in AF and obesity microarrays were identified, and these genes were enriched in inflammation- and immune-related function. The enriched cells were whole blood, CD33+ myeloid, and CD14+ monocytes. The hub genes were identified as MNDA, CYBB, CD86, FCGR2C, NCF2, LCP2, TLR8, HLA-DRA, LCP1, and PTPN22. All hub genes were only elevated in blood samples of obese-AF patients. The CIBERSORT analysis revealed that the AF patients’ left atrial appendage had increased infiltration of naïve B cells and decreased infiltration of memory B cells. The hub genes were related positively to naïve B cells and negatively to memory B cells. Ten hub genes may serve as biomarkers for obesity-related AF. These findings may also aid in comprehending pathophysiological mechanisms for obesity-related AF.

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WEI, CHUN, QIU-SHAN GAO, YONG-YANG GONG, XUE-MEI XIONG, and XIN SHEN. "SYNTHESIS AND CHARACTERIZATION OF A RODLIKE LIQUID CRYSTALLINE POLYURETHANE OLIGOMER." Functional Materials Letters 03, no. 03 (September 2010): 169–72. http://dx.doi.org/10.1142/s1793604710001172.

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A novel rodlike liquid crystalline polyurethane (LCPU) oligomer was synthesized by polyaddition of hexane-1,6-diylbis(4-hydroxybenzoate) with toluene 2,4-diissocyanate in N, N′-dimethylformamide solution. The properties of the LCPU oligomer were investigated by FT-IR, DSC, TGA, POM, WAXD and SEM. The LCPU oligomer was a yellow powder at room temperature, which maintained a stable rodlike microstructure below its melting point, and had high crystallinity. The LCPU oligomer exhibited thermotropic liquid crystalline properties between 166–183°C, and the smectic-A phase with focal conic fan textures at the liquid crystal state was observed by POM. The decomposition temperature of the LCPU oligomer at 5% weight loss was 282°C.

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Tsai, Chen-Liang, Chih-Ying Changchien, Ying Chen, Hsin-Han Chang, Wen-Chiuan Tsai, Yi-Wen Wang, Kai-Chieh Chou, et al. "Accelerated Wound Healing and Keratinocyte Proliferation through PI3K/Akt/pS6 and VEGFR2 Signaling by Topical Use of Pleural Fluid." Cells 11, no. 5 (February 26, 2022): 817. http://dx.doi.org/10.3390/cells11050817.

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Impaired wound healing is an ongoing issue that cancer patients undergoing chemotherapy or radiotherapy face. Our previous study regarding lung-cancer-associated pleural fluid (LCPF) demonstrated its propensity to promote endothelial proliferation, migration, and angiogenesis, which are crucial features during cutaneous wound healing. Therefore, the current study aimed to investigate the effect of pleural fluid on cutaneous wound closure in vitro and in vivo using HaCaT keratinocytes and a full-thickness skin wound model, respectively. Both heart-failure-associated pleural fluid (HFPF) and LCPF were sequentially centrifuged and filtered to obtain a cell-free status. Treatment with HFPF and LCPF homogeneously induced HaCaT proliferation with cell cycle progression, migration, and MMP2 upregulation. Western blotting revealed increased PI3K/Akt phosphorylation and VEGFR2/VEGFA expression in HaCaT cells. When treated with the PI3K inhibitor, LCPF-induced keratinocyte proliferation was attenuated with decreased pS6 levels. By applying the VEGFR2 inhibitor, LCPF-induced keratinocyte proliferation was ameliorated by pS6 and MMP2 downregulation. The effect of LCPF-induced cell junction rearrangement was disrupted by co-treatment with a VEGFR2 inhibitor. Compared with a 0.9% saline dressing, LCPF significantly accelerated wound closure and re-epithelization when used as a dressing material in a full-thickness wound model. Histological analysis revealed increased neo-epidermis thickness and dermis collagen synthesis in the LCPF-treated group. Furthermore, LCPF treatment activated basal keratinocytes at the wound edge with the upregulation of Ki-67, VEGFA, and MMP2. Our preliminaries provided the benefit of wet dressing with pleural fluid to improve cutaneous wound closure through enhanced re-epithelization and disclosed future autologous application in cancer wound treatment.

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Basit, Sulman, and Khalid I. Khoshhal. "Clinical and genetic characteristics of Legg-Calve-Perthes disease." Journal of Musculoskeletal Surgery and Research 6 (December 3, 2021): 1–8. http://dx.doi.org/10.25259/jmsr_123_2021.

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Legg-Calve-Perthes disease (LCPD) is a known childhood form of idiopathic femoral head osteonecrosis. It is characterized by a sequence of events involving the capital femoral epiphysis. The disease process is associated with the disruption of the blood supply to the femoral head. In most cases, LCPD appears in a sporadic form. Occurrences of cases in families have also been reported, with some families having more than two affected individuals. The disease etiology is still unknown, however, various factors have been considered for the pathogenesis of LCPD, including very low body weight or short stature at birth, maternal smoking, and secondhand smoke exposure. Interaction of multiple environmental and genetic factors has also been postulated as an underlying player in the development of the disorder. Hypercoagulability may have a major role in LCPD development. Families segregating LCPD largely demonstrate autosomal dominant inheritance. Variants in coagulations genes (Factor 5 and Factor 2) and collagen encoding gene (COL2A1) have been linked to the disease. However, our knowledge of the LCPD pathogenic factors is limited. A better understanding of the association between LCPD and causative factors, for example, the role of hypercoagulability in osteonecrosis development, might lead to the development of improved treatments, to shorten the acute phase of the disease during childhood as well as to possibly reduce the long-term effects of osteoarthritis in adulthood. Detection of large-effect variants underlying LCPD may help in offering extended screening for all first-degree family members. In this review, we would like to discuss the etiological factors underlying LCPD with special emphasis on the role of coagulation factors and mutations in the genes encoding those coagulation factors.

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Wei, Wen-Bo, Sha-Jie Dang, Yong Li, and Zong-Zhi Liu. "Alterations of Serum Osteocalcin Levels in Patients with Legg-Calvé-Perthes." HIP International 27, no. 1 (November 14, 2016): 92–95. http://dx.doi.org/10.5301/hipint.5000431.

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Background Legg-Calvé-Perthes disease (LCPD) is a paediatric form of osteonecrosis that ultimately heals but will cause femoral head and acetabular deformities. The purpose of this study was to investigate the early serum measurement of osteocalcin in children with femoral head necrosis compared with that of healthy children. Methods 20 patients with LCPD (4 girls and 16 boys) and 20 healthy volunteers (5 girls and 15 boys) were enrolled. All patients did not have additional treatment. Blood samples were obtained from all patients pre-operatively in the morning. All cases had hip radiographs. The serum levels of osteocalcin comparisons between the LCPD patients and the healthy volunteers were performed using Wilcoxon signed-rank test. The Spearman rank correlation was used to assess correlation between LCPD grade and serum osteocalcin levels. Significance was set at p = 0.05. Results The 20 patients with LCPD (72.75 ± 24.92 ng/ml) had significantly higher serum osteocalcin levels compared with the healthy group (16.80 ± 4.04 ng/ml) (p≤0.01). Serum osteocalcin levels of different LCPD grades were significantly different (Spearman's p = 0.540, p = 0.014). Conclusions We observed a significant correlation between serum osteocalcin levels and LCPD. These results may be meaningful in clinical practice and to future studies.

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Apostolov, A. T., I. N. Apostolova, and J. M. Wesselinowa. "Magnetic and electric properties of multiferroic LiFeP2O7. Comparison with LiCrP2O7." Modern Physics Letters B 35, no. 09 (February 2, 2021): 2150158. http://dx.doi.org/10.1142/s021798492150158x.

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The temperature and magnetic field dependence of the magnetic and electric properties of LiFeP2O7 (LFPO) and LiCrP2O7 (LCPO) are studied using a microscopic model and the Green’s function technique. We have shown that LFPO is antiferromagnetic, but shows a weak ferromagnetism along the [Formula: see text] axis which originates from the canted antiferromagnetic order. For LCPO, such a ferromagnetic order along the [Formula: see text] axis is not observed. In the temperature dependence of the electrical polarization [Formula: see text] along the [Formula: see text] axis there is a kink at [Formula: see text] K which is an indirect evidence for the intrinsic magnetoelectric effect in LFPO. Applying an external magnetic field [Formula: see text], the polarization [Formula: see text] increases, stronger for small temperatures and the kink at [Formula: see text] disappears. For LCPO, we do not obtain a kink at [Formula: see text] K. LCPO is polar, but not ferroelectric. We can conclude that the missing magnetoelectric properties in LCPO could be due to the differences in the magnetic orders between LFPO and LCPO.

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Asadollahi, Samira, Hossein Neamatzadeh, Nasim Namiranian, and Mohammad Reza Sobhan. "Genetics of Legg-Calvé-Perthes Disease: A Review Study." Journal of Pediatrics Review 9, no. 4 (October 1, 2021): 301–8. http://dx.doi.org/10.32598/jpr.9.4.964.1.

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Background: Legg-Calvé-Perthes Disease (LCPD), a juvenile hip disorder, is caused by impaired blood flow to the femoral head. In severe LCPD cases, the femoral head may develop a flattening deformity. Furthermore, if LCPD is diagnosed at the later stages, it causes early osteoarthritis of the hip. The etiology of LCPD is complex and embraces both genetic and epigenetic factors. Objectives: This review attempts to summarize the current knowledge on the role of these genetic variants in the incidence of LCPD. Methods: We searched for articles published in English using the special related search terms. Results: The genetic causes of this disease include mutations in the genes of thrombophilia factors, such as FV Leiden and anticardiolipin antibodies. The mutations of COL2A1, TRPS1, eNOS genes are the other causes. Moreover, the clinical symptoms of avascular necrosis may be indiscernible in patients with Gaucher’s disease or LCPD, and the differential diagnosis is a challenge. Conclusions: The results indicated that genetic testing may be useful in diagnosing and managing patients with juvenile hip disorders.

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Lee, Seung Hyen, Tae-Woo Kim, Eun Ji Lee, and Hyunkyung Kil. "Association between Optic Nerve Sheath Diameter and Lamina Cribrosa Morphology in Normal-Tension Glaucoma." Journal of Clinical Medicine 12, no. 1 (January 2, 2023): 360. http://dx.doi.org/10.3390/jcm12010360.

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(1) Background: To compare optic nerve sheath diameter (ONSD) in normal-tension glaucoma (NTG) and healthy eyes and to investigate the association between ONSD and lamina cribrosa (LC) morphology. (2) Methods: This cross-sectional study included 69 NTG eyes and 69 healthy eyes matched for age, axial length, and intraocular pressure. The LC curvature index (LCCI) was measured from horizontal Cirrus HD-OCT B-scan images from five uniformly divided positions vertically of the optic nerve. The average LCCI was defined as the mean of the measurements at these five locations. ONSD was measured as the width of the optic nerve sheath at the site perpendicular 3 mm behind the posterior globe. LCCI and ONSD were compared in eyes with NTG and healthy eyes. The clinical factors that could affect LCCI were analyzed. (3) Results: NTG eyes had significantly smaller mean ONSD (4.55 ± 0.69 mm vs. 4.97 ± 0.58 mm, p < 0.001) and larger average LCCI (11.61 ± 1.43 vs. 7.58 ± 0.90, p < 0.001) than matched healthy control eyes. LCCI was significantly correlated with smaller ONSD, higher intraocular pressure, thinner global retinal nerve fiber thickness, and worse visual field loss in all subjects (all Ps ≤ 0.022). (4) Conclusions: NTG eyes had smaller ONSD and greater LCCI than healthy control eyes. In addition, a negative correlation was observed between ONSD and LCCI. These findings suggest that cerebrospinal fluid pressure, which ONSD indirectly predicts, may affect LC configuration. Changes in the retrolaminar compartment may play a role in glaucoma pathogenesis.

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