Дисертації з теми "Laser Fluorescence Imaging"
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Tecu, Kirk S. "Laser induced fluorescence imaging of counterflow diffusion flames /." free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9841342.
Повний текст джерелаSasaki, K., S. Matsui, H. Ito, and K. Kadota. "Dynamics of laser-ablation Ti plasmas studied by laser-induced fluorescence imaging spectroscopy." American Institute of Physics, 2002. http://hdl.handle.net/2237/7045.
Повний текст джерелаCapewell, Dale L. Goodwin David G. Goodwin David G. "Planar laser induced fluorescence imaging and Monte Carlo simulations of pulsed laser ablation /." Diss., Pasadena, Calif. : California Institute of Technology, 1997. http://resolver.caltech.edu/CaltechETD:etd-01102008-095243.
Повний текст джерелаOmbinda-Lemboumba, Saturnin. "Laser induced chlorphyll fluorescence of plant material." Thesis, Stellenbosch : University of Stellenbosch, 2007. http://hdl.handle.net/10019.1/3064.
Повний текст джерелаImaging and spectroscopy of laser induced chlorophyll fluorescence (LICF) are emerging as useful tools in plant physiology and agriculture since these methods allow an early detection of plant stress and transformation of plant tissue, before visual symptoms appear. Chlorophyll fluorescence is governed by photosynthetic efficiency and it depends on the plant species and physiological state. In addition, the laser induced fluorescence of chlorophyll molecules in the red and far red spectral range is also used to study basic processes and phenomena in photo-excited molecules. In the work reported here experimental setups used for laser induced chlorophyll fluorescence imaging and spectroscopy techniques were developed to investigate chlorophyll fluorescence under constant illumination and also to detect green-fluorescent protein (GFP) by looking at the chlorophyll fluorescence spectrum and image. He-Ne (wavelength 632 nm), tunable argon ion (wavelength 455 nm), and excimer (wavelength 308 nm) lasers were used as excitation sources. An Ocean Optics spectrometer was used to record the spectrum of the chlorophyll fluorescence and the variation of the chlorophyll fluorescence spectrum with time. The chlorophyll fluorescence spectrum of tobacco leaves expressing GFP was compared to that of control leaves. A charge-coupled device (CCD) camera was used to image the fluorescence from GFP expressing and control tobacco leaves to investigate the effect of GFP genes on chlorophyll fluorescence in relation to the state of the plant material. The spectral analysis technique and image processing procedures were elaborated in order to obtain better information on chlorophyll fluorescence. The results of this work show that the experimental setups and analytical procedures that were devised and used are suitable for laser induced chlorophyll fluorescence analysis. Fluorescence bleaching could be obtained from the time variation of the fluorescence spectrum, and plant expressing GFP can be distinguished from control plants by differences in the laser induced chlorophyll fluorescence.
Jiang, Naibo. "Development of high repetition rate no planar laser induced fluorescence imaging." The Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=osu1150140816.
Повний текст джерелаElson, Daniel S. "Development of ultrafast laser technology and its application to fluorescence lifetime imaging." Thesis, Imperial College London, 2003. http://hdl.handle.net/10044/1/12005.
Повний текст джерелаAuksorius, Egidijus. "Multidimensional fluorescence imaging and super-resolution exploiting ultrafast laser and supercontinuum technology." Thesis, Imperial College London, 2009. http://hdl.handle.net/10044/1/4201.
Повний текст джерелаLanigan, Peter Michael Pinto. "Applications of confocal and multiphoton laser scanning microscopes to multi-dimensional fluorescence imaging." Thesis, Imperial College London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.439847.
Повний текст джерелаSasaki, K., T. Wakasaki, S. Matsui, and K. Kadota. "Distributions of C_2 and C_3 radical densities in laser-ablation carbon plumes measured by laser-induced fluorescence imaging spectroscopy." American Institute of Physics, 2002. http://hdl.handle.net/2237/7043.
Повний текст джерелаBerckmuller, Martin. "A study of mixture formation in a lean burn research engine using laser fluorescence imaging." Thesis, Cranfield University, 1996. http://dspace.lib.cranfield.ac.uk/handle/1826/9933.
Повний текст джерелаBerkmuller, Martin. "A study of mixture formation in a lean burn research engine using laser fluorescence imaging." Thesis, Cranfield University, 1996. http://dspace.lib.cranfield.ac.uk/handle/1826/10431.
Повний текст джерелаFuller, Joseph P. "High-frame-rate planar laser-induced fluorescence imaging of diesel sprays using pulse burst diagnostics." [Ames, Iowa : Iowa State University], 2009.
Знайти повний текст джерелаYamamoto, Ryuta. "A Quantum Gas Microscope of Two-electron Atoms with Fluorescence and Faraday Imaging." Kyoto University, 2016. http://hdl.handle.net/2433/217727.
Повний текст джерелаStaufer, Theresa Maria [Verfasser], and Florian [Akademischer Betreuer] Grüner. "X-Ray Fluorescence Imaging with a Laser-Driven X-Ray Source / Theresa Maria Staufer ; Betreuer: Florian Grüner." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2020. http://d-nb.info/1217408878/34.
Повний текст джерелаMoses, Lance. "Fluorescence Imaging of Analyte Profiles in an Inductively Coupled Plasma with Laser Ablation as a Sample Introduction Source." BYU ScholarsArchive, 2015. https://scholarsarchive.byu.edu/etd/4367.
Повний текст джерелаBrümmer, Theresa [Verfasser], and Florian [Akademischer Betreuer] Grüner. "Design Study of a Laser-Driven X-ray Source for Medical Fluorescence Imaging / Theresa Brümmer ; Betreuer: Florian Grüner." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2018. http://d-nb.info/1160036063/34.
Повний текст джерелаBrümmer, T. [Verfasser], and Florian [Akademischer Betreuer] Grüner. "Design Study of a Laser-Driven X-ray Source for Medical Fluorescence Imaging / Theresa Brümmer ; Betreuer: Florian Grüner." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2018. http://nbn-resolving.de/urn:nbn:de:gbv:18-91354.
Повний текст джерелаLiu, Quan. "Planar laser induced fluorescence imaging and analysis with ethanol blended fuels in a direct injection spark ignition engine." Thesis, Brunel University, 2017. http://bura.brunel.ac.uk/handle/2438/14786.
Повний текст джерелаKranz, Patrick [Verfasser], and Sebastian [Akademischer Betreuer] Kaiser. "Imaging of mixing processes in spark-ignition engines via quantitative laser-induced fluorescence / Patrick Kranz ; Betreuer: Sebastian Kaiser." Duisburg, 2020. http://d-nb.info/1218465352/34.
Повний текст джерелаBélisle, Jonathan. "Design and assembly of a multimodal nonlinear laser scanning microscope." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=100765.
Повний текст джерелаEdmund, Alisa Jane. "Laser-Induced Fluorescence Imaging of Calcium and Barium Ion Beams in the Second Vacuum Stage of a Commercial Inductively Coupled Plasma Mass Spectrometer." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/4135.
Повний текст джерелаVilmart, Gautier. "Détection de vapeurs d'atomes métalliques par fluorescence induite par laser (LIF) : application à la propulsion solide." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS523/document.
Повний текст джерелаDuring the PhD thesis, high-speed laser induced fluorescence (LIF) of two metallic atoms (Al and Fe) is presented, in order to use them as fluorescent markers in solid propellant flames, where they are naturally present. LIF measurements are first performed inside two different evaporation chambers used to generate aluminum vapors in controlled conditions over a broad pressure and temperature range. A theoretical model of the LIF process is elaborated and applied to both atoms in order to calculate the signal quenching rate as a function of pressure and temperature. Unavailable collisional data are determined theoretically for Fe and experimentally for Al. Energy transfer and collisional broadening coefficients are determined experimentally for the Al atom in pure nitrogen environment. Study of the signal level of Al as a function of laser intensity is undertaken to measure saturation thresholds in N₂, He and Ar as a function of pressure. The model is used to properly reproduce the temporal and spectral profiles, though some approximations and limitations remain. A first application of high-speed LIF imaging to the measurement of aluminum in a solid propellant flame (10 bar , 3000 K) is demonstrated. It allows us to clearly visualize reactive aluminum droplets in the flame and to follow their evolution in the flame
Davoudi, Henrik. "Characterisation of mixture preparation in lean premixed prevaporised combustor modules by planar laser fluorescence imaging and computational fluid dynamics modelling." Thesis, Cranfield University, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393697.
Повний текст джерелаWall, Richard Andrew. "Multi-Modality Endoscopic Imaging for the Detection of Colorectal Cancer." Diss., The University of Arizona, 2013. http://hdl.handle.net/10150/301761.
Повний текст джерелаPalmer, Johannes [Verfasser], Reinhold [Akademischer Betreuer] Kneer, and Frank [Akademischer Betreuer] Beyrau. "A laser-induced fluorescence technique for temperature imaging of micro-droplets avoiding morphology dependent resonances / Johannes Palmer ; Reinhold Kneer, Frank Beyrau." Aachen : Universitätsbibliothek der RWTH Aachen, 2019. http://d-nb.info/121778974X/34.
Повний текст джерелаWatson, Jeffrey R., Summer Garland, and Marek Romanowski. "Intraoperative visualization of plasmon resonant liposomes using augmented microscopy." SPIE-INT SOC OPTICAL ENGINEERING, 2017. http://hdl.handle.net/10150/625390.
Повний текст джерелаDunand, Pierre. "Étude de l'impact de goutte sur une paroi chaude en régime de Leidenfrost." Thesis, Université de Lorraine, 2012. http://www.theses.fr/2012LORR0166/document.
Повний текст джерелаThe droplet impact onto a heated wall interaction can be found in mutiples fields, such as internal combustion engines or the steel industry, specially in the thermal treatment of the steel where high energy dissipation rate is required. The study of this interaction should grant a better understanding of this phenomena, and thus, allow the enhancement of these processes and reduce the energy and water consumption. Several studies have already been carried out on this subjet but a great majority of them, whose results present great divergence, only consider the heated wall, neglecting the outgoing of the droplet. This thesis put the emphasis on the liquid phase where currently no data exist to our knowledge, with the help of multiples experimental technics that have been developped. A general experimental setup has been made in order to make all the wanted measurements. The measurement techniques used in this study are first presented, to begin with the planar laser induced fluorescence, which allow us to know the droplet temperature during the impact. Used with an infrared thermography technique coupled with an inverse conduction model, it is possible to extract some important parameter regarding the thermal aspect, such as the droplet heating, the relative evaporation ratio or the cooling efficiency. This latter can be written as the sum of the two major contributions of the cooling: the sensible heat and the evaporation of the droplet. Finally, a high speed shadow imagery technique is presented. It has been developped in order to study the dynamic aspect of the droplet impact. This technique can determine several dynamic parameters such as the droplet speed and diameter, or other parameter regarding the type of impact encountered
Guiberti, Thibault. "Analyse de la topologie des flammes prémélangées swirlées confinées." Thesis, Châtenay-Malabry, Ecole centrale de Paris, 2015. http://www.theses.fr/2015ECAP0014/document.
Повний текст джерелаThis work deals with the stabilization of premixed turbulent swirling flames of methane/hydrogen/air combustible mixtures with different dilution rates of nitrogen and carbon dioxide. A central bluff body helps stabilizing the flames at low swirl numbers. The flame tip eventually impinges the combustor peripheral wall. The general objective is to gain understanding of the mechanisms governing the stabilization and the topology of these flames. It is found that the swirl number, the combustible mixture composition, the geometry of the combustor, and the thermal boundary conditions have a strong impact on the shape taken by these flames. The experimental setup used to characterize flames topologies is first described. Flames prone to topology bifurcations are selected and are studied for different arrangement of the combustor when the combustion chamber shape and size, the injection tube diameter, and swirl number are varied. One operating condition is fully characterized under non-reactive and reactive conditions using Planar Hydroxyl Laser Induced Fluorescence (OH-PLIF), Particle Imaging Velocimetry (PIV), and Laser Induced Phosphorescence of thermographic phosphors (LIP) to generate a detailed database of the flow and the corresponding boundary conditions. An analysis is then conducted to understand the mechanisms controlling shape bifurcations when the flame interacts with the combustor peripheral wall. Effects of the combustible mixture composition, the bulk flow velocity, and the swirl number are analyzed. It is shown that the transition from a V to an M flame is triggered by a flashback of the V flame tip in the boundary layer of the combustor peripheral wall. Dimensionless numbers controlling these transitions are identified and a simplified model is developed to help the prediction of the flame shapes. The physics of these shape bifurcations differs when the flame does not interact with the combustor wall. The large influence of the hydrogen enrichment in the fuel on the flame shape is analyzed using flame chemiluminescence and OH-PLIF. LIP and thermocouple measurements demonstrate that the thermal boundary conditions still have a strong impact on the flame topology. The combined effects of strain and heat losses are investigated using joint OH-PLIF and PIV experiments. It is shown that flammability limits of premixed flames are reduced due to heat losses and the transitions from M to V shaped flames is consecutive to localized extinctions of flame front elements located in the outer shear layer of the jet flow that are submitted to large strain rates. These experiments are completed by an analysis of the dynamics of methane/hydrogen/air flames. It is shown that low frequency and high amplitude velocity modulations generated by a loudspeaker alter the shape taken by these flames. The stabilization of methane/hydrogen/air flames diluted by nitrogen and carbon dioxide is finally examined. It was possible to stabilize swirled flames featuring important dilution rates due to the presence of the bluff body, installed on the axis of the injection tube. The recirculation zone behind this element supplies hot burnt gases to the flame anchoring point. Using OH* chemiluminescence imaging, it is shown than increasing the molar fraction of diluent in the fuel reduces the light emission from excited OH* radicals. The influence of dilution on the flame chemistry is emphasized with experiments conducted at a fixed thermal power and fixed adiabatic flame temperature. It is also demonstrated that the composition of the diluent has a strong influence on the temperature field of the burnt gases and of the combustor wall surfaces. Dilution with carbon dioxide increases radiative heat losses from the burnt gases in comparison to dilution with nitrogen. This penalizes the combustor efficiency equipped with four transparent quartz walls. [...]
Cosimi, Julien. "Caractérisations d'un jet de plasma froid d'hélium à pression atmosphérique." Thesis, Toulouse 3, 2020. http://www.theses.fr/2020TOU30136.
Повний текст джерелаCold atmospheric pressure plasma jets are a subject of great interest in many biomedical fields for the past decade. In the various applications of these jets, the plasma generated can interact with many types of surfaces. Plasma jets influence the treated surfaces, but it is now well known that the treated surface also influences the plasma according to their characteristics. The work carried out in this thesis therefore aims to characterize a cold helium atmospheric pressure plasma jet in contact with three surfaces (dielectric, metallic and ultrapure water) by means of different electrical and optical diagnostics in order to understand the influence of the nature of the surfaces on the physical properties of the plasma and the chemical species generated. The first part of this thesis is focused on the study of the influence of surfaces on the plasma jet. Different parameters are studied, such as the nature of treated surfaces, the gas flow, the distance between the outlet of the device and the surface or the composition of the injected gas. For this purpose, helium flow at the outlet of the device is followed by Schlieren imagery with and without the discharge. Emission spectroscopy is used to determine the emissive species generated by the plasma. ICCD imagery is employed to follow the generation and the propagation of the discharge and the distribution of several excited species in the jet by using band-pass interference filters. A dielectric target causes the ionization wave to spread over its surface and a conductive target leads to the formation of a conduction channel. The evolution of excited species densities (OH*, N2*, He* and O*) increases with the relative permittivity of the treated surface. As well known, active species generated by plasma jets play a fundamental role in the kinetics and the chemistry of the mechanisms linked to plasma processes. The second part of the present work therefore relates to the spatial and temporal evaluation of the densities of the hydroxyl radical OH which plays a major role in many cellular mechanisms. The spatial mapping and the temporal evolution of the absolute and relative densities of OH are obtained by LIF and PLIF laser diagnostics. The density of OH generated increases with the electrical conductivity of the treated surface. It can be noted that the OH molecules remain present in the helium channel between two consecutive discharges (several tens of microseconds). Finally, we focus on the production of chemical species in ultrapure water treated with plasma. The influence of different parameters on the concentration of species in the treated water has been studied to optimize the production of chemical species. In experimental conditions, grounding the ultrapure water during treatment increases the concentration of H2O2. Furthermore, the grounding induces a decrease in the NO2- concentration
Tawidian, Hagop-Jack. "Formation et comportement de nanoparticules dans un plasma : instabilités dans les plasmas poudreux." Thesis, Orléans, 2013. http://www.theses.fr/2013ORLE2033/document.
Повний текст джерелаThe objective of this thesis is to study the formation of carbonaceous nanoparticles in a low pressure plasma. Dust particles are created by sputtering a polymer layer deposited on the bottom electrode of a capacitively coupled radio-frequency discharge. The presence of dust particles disturbs and changes the plasma properties. The growth of dust particles can trigger low frequency instabilities that evolve with the dust particle size and density. In the center of the discharge, the void, a dust-free region, is observed. It is characterized by an enhanced luminosity. Different diagnostics (electrical measurements, high speed imaging, Laser Induced Fluorescence) are used in order to understand these different behaviors resulting from plasma-dust particle interactions. Dust particle growth instabilities are investigated showing the existence of different instability regimes. Their main characteristics are extracted such as their duration and their evolution frequency. These instabilities are characterized by the formation of small plasma spheroids moving and interacting in the discharge. Several interesting phenomena are evidenced such as the merging and splitting of these plasma spheroids. Concerning the void, our investigations confirmed the high excitation occurring in this region. In the last part of the thesis, the dissociation of aluminium triisopropoxide (ATI) is studied in a plasma using Fourier Transform InfraRed spectroscopy. Thanks to this diagnostic, the evolution of ATI density has been studied as a function of the discharge parameters. We have also quantified the different hydrocarbon compounds formed by polymerization
Lee, Woei Ming. "Optical trapping : optical interferometric metrology and nanophotonics." Thesis, St Andrews, 2010. http://hdl.handle.net/10023/882.
Повний текст джерелаDowling, Keith. "Application of ultrafast lasers to fluorescence lifetime imaging." Thesis, Imperial College London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313763.
Повний текст джерелаThompson, James Russell. "Imaging the assembly of the Staphylococcal pore-forming toxin alpha-Hemolysin." Thesis, University of Oxford, 2009. http://ora.ox.ac.uk/objects/uuid:e320004a-6118-4dac-af2a-eca6e90be7ac.
Повний текст джерелаBookjans, Eva M. "Relative number squeezing in a Spin-1 Bose-Einstein condensate." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/37148.
Повний текст джерелаChadwick, Helen J. "Angular momentum polarisation effects in inelastic scattering." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:474b04fa-4f50-4618-88ab-c85878723f2a.
Повний текст джерелаDo, Le Duy. "Relation entre l’annexine A6 et la phospholipase D1 pendant le processus d’exocytose dans les cellules PC12." Thesis, Lyon 1, 2014. http://www.theses.fr/2014LYO10160/document.
Повний текст джерелаThe regulated exocytosis is a key process allowing cell-cell communication through the release of hormone and neurotransmitters. In neurons and neuroendocrine cells, it is strictly controlled by extracellular signal such as transmembrane potential and ligand bindings to receptors. Substantial progress has been made to understand the molecular mechanism of exocytosis. Major components of secretory machinery have been brought to light. Now the emergent question concerns the role of scaffolding proteins that are thought to coordinate the action of each other. In the case of annexin family well known to be involved in exocytosis, their modes of –sequential or concerted- interactions with other proteins, and their regulatory effects on exocytosis are not very well established. Previous findings indicated that Annexin A6 (AnxA6) affected calcium homeostasis and dopamine secretion from PC12 cells, used as cellular model of neurosecretion (Podszywalow-Bartnicka et al., 2010). To determine the inhibitory effect of AnxA6 on exocytosis of dopamine, we were looking for molecular partners of AnxA6 in PC12 cells. We hypothesized that AnxA6 interacts with phospholipase D1 (PLD1), an enzyme involved in the fusion step. By using confocal microscopy and total internal reflection fluorescence microscopy, we found that isoform 1 of AnxA6 and Phospholipase D1 are both recruited on the surface of vesicles upon stimulation of PC12 cells. AnxA6 inhibited phospholipase D activity as revealed by our enzymatic assay based on infrared spectroscopy. To conclude, we propose that AnxA6 is not only implicated in membrane organization by its capacity to bind to negative charged phospholipids and to cholesterol, but AnxA6 is also affecting PLD1 activity, changing membrane lipids composition
Crépeau, Joël. "Development of a single-mode interstitial rotary probe for In Vivo deep brain fluorescence imaging." Thesis, Université Laval, 2013. http://www.theses.ulaval.ca/2013/29428/29428.pdf.
Повний текст джерелаThis thesis documents the expertise developed by the author at the Centre de recherchede l’Institut universitaire en santé mentale de Québec (CRIUSMQ) in fibered endoscopy,particularly the design and construction of a new kind of optical microscope: ThePanoramic Interstitial Microscope (PIM). Through the juxtaposition of a short piece ofGraded-Index fibre and a prism at the end of a single-mode fibre, laser light is focussedon the side of the probe. To form an image, the latter is quickly spun around its axiswhile it is being pulled vertically by a piezoelectric actuator. This minimally invasivefluorescence rotary interstitial imaging system is an endeavor to limit the damage causedby the probe while imaging enough tissue to provide good context to the user in deep brain optical imaging.
Biasiori-Poulanges, Luc. "Fragmentation aérodynamique d’une goutte liquide induite par une onde de choc plane High-magnification shadowgraphy for the study of dropbreakup in a high-speed gas flow On the formation and recurrent shedding of ligaments in droplet aerobreakup Data on eosin y solutions for laser-induced fluorescence in water flows Multimodal imaging for intra-droplet gas-cavity observation during droplet fragmentation." Thesis, Chasseneuil-du-Poitou, Ecole nationale supérieure de mécanique et d'aérotechnique, 2021. http://www.theses.fr/2021ESMA0002.
Повний текст джерелаThis thesis proposed a groundbreaking description of the shock-induced aerodynamic fragmentation of a water droplet at the transition of the Rayleigh-Taylor Piercing and the Shear-Induced Entrainment regimes. An experimental facility consisting of a shock tube and high-speed imaging diagnostics is used to investigate the fragmentation processes. Experimental results are supported with numerical simulations performed with the open-source code ECOGEN dedicated to multiphase compressible flows. The shock wave effect on the droplet is assessed by a theoretical modelling based on geometrical acoustics which allows for the description of the wave spatio-temporal dynamics and enables to predict the time-dependent location of the highest energy density. Pressure fields are determined using numerical simulations. It appears that the water tensile rupture is reached for a shock wave Mach number of 1.7 from which bubble cloud cavitation may occur by causing signification changes in the fragmentation dynamics. As regards to the interfacial dynamics, both experiments and numerical simulations show the development of a transverse azimutal modulation resulting in the periodic ligament structure at the droplet surface. Contrary to the modulation growth, its initiation seems to be independent of the capillary effects as revealed by a Fourier analysis of the 3-D numerical results. The ligament dynamics is a cyclic process driven by the vortex shedding process in the wake of the droplet. Four cycles have been observed before the residual droplet core breaks up owing to the growth of an air cavity inside the droplet that acts as weak spot, and thus facilitating the droplet split-off
Banerjee, Amartya. "Beta-Peptide Helices As Transmembrane Domains: Aggregation, Recognition and Lipid-Peptide Interaction." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E56E-5.
Повний текст джерелаSeptiadi, Dedy. "Optical imaging and drug delivery using soft- and hard- nanomaterials." Thesis, Strasbourg, 2015. http://www.theses.fr/2015STRAF036/document.
Повний текст джерелаThe work described in this thesis focuses on the development of soft- and hard-materials as well as their interaction with biological cells for applications in the field of theranostics covering imaging, sensing, and gene, and cancer therapy. In this context, we first investigated the use of phosphorescent self-assembled platinum(II) complexes as cellular probes. We extended the concept stimulated emission-based bioimaging by generating a laser-like emission coming from a single biological cell without using any conventional optical cavity. In addition, we successfully developed multifunctional nanocarriers based on porous hard materials, namely zeolites-L and mesoporous silica nanoparticles for drug and oligonucleotide delivery in vitro and they were tested to treat glioblastoma. Another nanovector, which is constructed from biodegradable silica, was also synthesized and its ability to encapsulate proteins and release them in living cells upon degradation of the structure in reductive environment was demonstrated. Finally, the use of novel plasmonic structures based on breakable silica-coated silver nanoparticles for detection of reducing agents was successfully investigated
Moreau, David. "Infrared stimulation of neurons." Thesis, Limoges, 2017. http://www.theses.fr/2017LIMO0050/document.
Повний текст джерелаInfrared laser light radiation may be used to depolarize neurons and to stimulate neural activity. The underlying mechanism of such stimulation is believed to happen due to a photothermal interaction. The absorption of the infrared radiation by the targeted biological tissue inducing a local temperature increase which either directly influence membrane properties or act via temperature sensitive ion channels. Action potentials are typically measured electrically in neurons with microelectrodes, but they can also be observed using fluorescence microscopy techniques that use synthetic or genetically encoded calcium indicators. In this work, we studied the impact of infrared laser light on neuronal calcium signals to address the mechanism of these thermal effects. HT22 mouse hippocampal neurons and U87 human glioblastoma cells were used loaded with the fluorescent calcium dye Fluo-4 and with the temperature sensitive fluorophore Rhodamine B to measure calcium signals and temperature changes at the cellular level. Here we present our all-optical strategy for studying the influence of infrared laser light on neural activity, and the scientific approach leading to conclusion of the involvement of Phospholipase C activity during infrared neural stimulation. The ability of infrared exposure to trigger neural activity in mice brain in vivo is also investigated with the use of GCaMP6s transgenic mice
Palma, Philip Charles. "Laser-induced fluorescence imaging in free-piston shock tunnels." Phd thesis, 1999. http://hdl.handle.net/1885/144483.
Повний текст джерелаCapewell, Dale L. "Planar Laser Induced Fluorescence Imaging and Monte Carlo Simulations of Pulsed Laser Ablation." Thesis, 1997. https://thesis.library.caltech.edu/111/3/Capewell_dl_1997.pdf.
Повний текст джерелаThe first reported planar laser-induced fluorescence (PLIF) images depicting the relative ground-state, neutral density of Si within a pulsed laser ablation (PLA) plume as it expands into vacuum and 10 mTorr Ar are presented. Because of the high flow speeds in PLA plumes and the narrow bandwidth of typical lasers, several images acquired with the laser detuned incrementally must be superimposed to produce images which compensate for Doppler shifts. Density profiles of Si along the axis of symmetry as a plume expands into Ar at pressures of 0-150 mTorr demonstrate the influence of a background gas and substrate on the expanding plume in the interval 0 < t < 5μs. In-flight production of SiO along the contact as an Si plume expands into 1.0 Torr air is demonstrated in the first reported images of a reactive intermediate species produced during PLA.
The expansion of Si into both 10 mTorr and 100 mTorr Ar is investigated using the technique of Direct Simulation Monte Carlo (DSMC), with a simple ablation model used to generate the Si plume. As a consequence of the rapid displacement of background gas, i.e. the snowplow effect, the plume divides into two components, one which travels nearly collisionlessly toward the substrate and one which interacts more strongly with the gas. At 10 mTorr, collisions between fast component Si atoms and Ar atoms during the period t < 250 ns are responsible for most of the energy transfer from Si to Ar. This results in the creation of a fast (15 eV), collisionless Ar component which impacts the substrate shortly after the fast Si component arrives, but does not result in thermal heating. At 100 mTorr, the energy transfer from the plume to the Ar gas occurs on a μs time scale and is attributed primarily to thermal heating. The energy of the fast Ar component decreases to about 5 eV.
Finally, the DSMC technique is employed to study the expansion of Si into both 10 mTorr and 100 mTorr O2. The chemical reaction Si + O2 → SiO + O is investigated, including rotational and vibrational excitation the diatomic molecules O2 and SiO. At both pressures, SiO first appears along the sides of the expanding plume and, at 100 mTorr, appears only along the contact front between the Si and O2. This result compares well will PLIF data. An examination of the O2 rotational temperature indicates that the background gas is heated along a conical surface whose tip is located at the ablation spot as the plume expands.
Collectively, these simulations demonstrate the potential of the DSMC method to provide quantitative information about the reactive expansion of a wide variety of PLA plumes into rarified background gas mixtures, and study the particle flux onto a substrate when parameters such as background gas pressure and composition are varied.
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