Дисертації: "Laser blood flow meter"

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FOLGOSI, CORREA MELISSA S. "Caracterização das flutuações do sinal doppler do fluxo microvascular." reponame:Repositório Institucional do IPEN, 2011. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10032.

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Tese (Doutoramento)
IPEN/D
Instituto de Pesquisas Energéticas e Nucleares - IPEN-CNEN/SP

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Чорний, Владислав Олександрович. "Лазерний вимірювач швидкості кровотоку". Bachelor's thesis, КПІ ім. Ігоря Сікорського, 2021. https://ela.kpi.ua/handle/123456789/43661.

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Обсяг звіту становить 56 сторінок, міститься 25 ілюстрацій, 17 таблиці. Загалом опрацьовано 22 джерел. Актуальність: контроль та швидка оцінка характеристик параметрів кровотоку є важливим атрибутом правильної діагностики пацієнта, а в особливості неівазивним методом вимірювання, так як сприйняття організму чужорідного тіла може вести до неприємних наслідків. Мета: недорогий та надійний лазерний вимірювач швидкості кровотока, як для лабораторних дослідів, так і для повсякденного контролю пацієнта. Завдання: 1. Огляд та аналіз літератури, що стосується лазерних вимірювачів швидкості кровотоку. 2. Огляд та аналіз інтелектуальної власності сучасних лазерних вимірювачів швидкості кровотоку. 3. Побудова оптично-функціональної схеми приладу. 4. Підбір елементів для реалізації швидкоміра. 5. Моделювання лазерного вимірювача кровотоку.
The volume of the report is 56 pages, contains 25 illustrations, 17 tables. In general, 22 sources were processed. Relevance: control and rapid assessment of the characteristics of blood flow parameters is an important attribute of proper diagnosis of the patient, and in particular a non-invasive method of measurement, as the perception of a foreign body can lead to unpleasant consequences. Purpose: inexpensive and reliable laser blood flow meter, both for laboratory experiments and for daily monitoring of the patient. Task: 1. Review and analysis of the literature related to laser blood flow meters. 2. Review and analysis of intellectual property of modern laser blood flow meters. 3. Construction of the optical-functional scheme of the device. 4. Selection of elements to the optical-functional scheme of the flowmeter. 5. Simulation of a laser blood flow meter.

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Diwei, He M. Res. "Full field laser doppler blood flow sensor." Thesis, University of Nottingham, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.523084.

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4

Kongsavatsak, Chayut. "Full field laser doppler blood flow camera." Thesis, University of Nottingham, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.489689.

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Laser Doppler Blood Flowmetry is a non-invasive technique that has been I developed and used for measuring microvascular blood flow in tissue. The technique utilises the backscattering of the laser light from moving red blood cells and static tissue in order to extract information such as the concentration and flow. This thesis describes the early stages of the development of an integrated optical sensor array to perform full field laser Doppler blood flow imaging. This technique will eliminate the need for mechanical scanning and the data bottleneck that exists between the photodiode array and processing unit, so allowing the direct measurement ofblood flow maps to be obtained in real time. A single channel laser Doppler blood flowmetry device has been implemented using a photodetector linked to a field programmable gate array. Filters (low pass, band pass and frequency weighted) have been developed for processing the Doppler signals to obtain flow and concentration measurements. The responses of these filters are demonstrated using measurements from modulated light signals, a rotating diffusing disc and in vivo measurements of blood flow. Several types of a linear array system and current to voltage converter are considered for the first fabrication run of the project based on the cost of fabrication and performance of the system such as operating frequency, gain, bandwidth and signal to noise ratio. A 16x1 linear array of photodiodes is developed and integrated on the same chip with the laser Doppler processing design, successfully implemented in the single channel laser Doppler system, using the standard 0.35Jlm CMOS technology. The characterisation of each individual part of the design was carried out and compared with the Cadence simulation results. The performance of the system on a single pixel is also evaluated using a modulated laser as a light source. The knowledge gained from the characterisation and the overall performance of the linear array system is then used to develop a full field laser Doppler blood flow camera.

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Sun, Shen. "Laser Doppler imaging and laser speckle contrast imaging for blood flow measurement." Thesis, University of Nottingham, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604304.

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The two blood flow imaging techniques, laser Doppler blood flow imaging (LDI) and laser speckle contrast blood flow imaging (LSCI), are well established and broadly applied in medical research. They are similar as both detect and process a fluctuating interference (speckle) pattem. However, the difference between processing algorithms provides different imaging characteristics. LDI can provide accurate, quantitative blood flow measurement which is seldom achieved by LSCI. Nevertheless, the fast imaging speed and simple instrumental setup provided by LSCI overcome some of the limitations ofLDI. With the development of high frame rate cameras full field LDI is now feasible and with the development of new processing algorithms LSCI is now providing more accurate quantitative information. It is therefore important to compare the performance of these two techniques. A full-field LDI system based on an FPGA (Field Programmable Gate Array) coupled with a high-speed CMOS (Complementary Metal-Oxide-Semiconductor) camera chip has been developed which provides blood flow images with flexible frame rates and spatial resolution. When a high spatial resolution is required, 1280xl024-pixel blood flow images were obtained by processing up to 2048 samples at O.2fps (frame per second). Altematively, a maximum of 15.5fps was achieved by reducing the resolution and sampling points to 256x256 pixels and 128 samples respectively. As a generic full-field LDI system, several parts of the system (memory unit, processing unit) can be simply updated or transplanted to another platform. The resource usage is optimized by utilizing a mixture of fixed and floating-pointing implementations, and the imaging speed is maximised because of the design of streamline structure which enables continuous input of data. Images were obtained of rotating diffusers at different rotation velocities and the system provides a linear relationship with velocity. Human blood flow images are also demonstrated both of the finger and of a healing wound. The author-designed LDI system was then applied to a high-spatial resolution flow imaging application in which the mixture of water and polystyrene micro spheres was pumped through a micropipette (diameter = 250llm) with controlled velocities, and the resulting flow was imaged and processed. The accurate, high-spatial resolution flow measurement was demonstrated by the resulting flow images which are of size 1280x 1 024 pixels and obtained by processing 2048 samples at each pixel. Besides the LDI system, a novel LSCI system has been developed on the same platform, establishing a unique LDI and LSCI hybrid system. By developing the LSCI method with equivalent exposures, the LDI data can be analysed using LSCl processing, enabling a truly fair comparison of these two methodologies. For comparison, measurements were carried out on a rotating diffuser that simulates the human tissue with controlled parameters. Although LDI and LSCI are qualitatively similar, the lack of quantitative blood flow measurement ofLSCI was recognized from the comparison since LSCI is exposure time dependent and unable to linearly detect the velocity changes. 11 To improve the linearity and accuracy ofLSCI measurement, multi-exposure laser speckle contrast imaging (MLSCI) has been introduced. However this increases image acquisition time as consecutive images at different exposure times need to be acquired. On the basis of the novel LSCI method, a new MLSCI scheme has been invented. The advantage of the MLSCI is that each frame is exposed with a fixed duration and various exposure times are alternatively achieved by accumulating several successive frames. In this way, the requirement to obtain a wide range of exposure times from consecutive images is overcome. This reduces image acquisition time as it depends on the longest exposure time rather than the sum of all exposures. From measurements of a rotating diffuser, the MLSCI was demonstrated to be capable of quantitatively measuring flow changes as in LDI. III

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Nguyen, Hoang Cuong. "High speed processing for laser doppler blood flow imaging." Thesis, University of Nottingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.517694.

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7

Godden, David J. "Measurement of airway blood flow by laser Doppler flowmetry." Thesis, University of Edinburgh, 1991. http://hdl.handle.net/1842/23933.

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Laser Doppler flowmetry (LDF) is a non-invasive method of measuring microcirculatory blood flow based on quantifying the Doppler frequency shift imparted to laser light by moving red blood cells. The object of this study was to examine whether a laser Doppler flow probe could be used to measure airway wall blood flow during bronchoscopy. Studies were performed in dogs, sheep and humans. Artifactual LDF flow signals were identified due to inadequate contact between the probe head and the mucosa, ventilatory and cardiac movement, and ambient light interference. Measurement technique was modified to minimise these artifacts. Site-to-site variation in LDF flow signals under baseline conditions was observed in all species (mean coefficient of variation = 36%), and, in humans, variation was similar in awake subjects during breath-holding, and in anaesthetised subjects during cardiopulmonary bypass, in whom ventilatory and cardiac artifacts are absent. When the probe was held at a single site, linear flow-pressure relationships (r = 0.63 - 0.9, p< 0.001) were observed in the trachea in 7 dogs during acute changes in mean systemic blood pressure and airway pressure. In 4 sheep, average LDF flow signals within regions of the bronchi varied in a linear fashion with changes in blood flow through a cannulated bronchial artery perfused by a roller pump. However, site-to-site variation in response occurred, and a substantial signal persisted when bronchial arterial flow was stopped, or when the artery was perfused by a cell-free solution of dextran which would, in theory, be expected to produce no LDF signal. These results may be explained in part by collateral blood flow, but also indicate detection of 'noise'. In 5 dogs, blood flow was measured in 6 regions of the trachea by both LDF and by the radiolabelled microsphere reference flow technique during resting ventilation (baseline), application of 15 cm H₂O positive end-expiratory pressure (PEEP) and during hyperventilation of dry air (HV). When regional measurements were examined, weak, but significant, correlations were observed between LDF and reference flow measurements during each condition. However, during PEEP, although both techniques indicated a similar mean reduction in blood flow (63%) from baseline, there was no correlation between the techniques in the magnitude of reduction measured in individual regions. During HV, LDF measurements showed variable responses between regions, and the mean change from baseline was not significantly different from zero. In contrast, reference flow values increased in most regions, and the mean increase was 87% from baseline. Sectioning of the tracheal wall indicated that this increase was localised to the mucosal layer. The results indicate that acute changes in blood flow at single sites in the airway may be detected by LDF applied in the present fashion. However, detection of 'noise' and site-to-site variation in LDF signals preclude quantitative measurements of airway wall blood flow using this probe design, particularly when the probe is moved between sites during an intervention.

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Shymkiw, Roxane Chia-Chi. "Measurement of blood flow in bone by laser Doppler imaging." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape3/PQDD_0021/MQ55267.pdf.

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9

Himsworth, John M. "Linear array CMOS detectors for laser Doppler blood flow imaging." Thesis, University of Nottingham, 2011. http://eprints.nottingham.ac.uk/12392/.

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Laser Doppler blood flow imaging is well established as a tool for clinical research. The technique has considerable potential as an aid to diagnosis and as a treatment aid in a number of situations. However, to make widespread clinical use of a blood flow imager feasible a number of refinements are required to make the device easy to use, accurate and safe. Existing LDBF systems consist of 2D imaging systems, and single point scanning systems. 2D imaging systems can offer fast image acquisition time, and hence high frame rate. However, these require high laser power to illuminate the entire target area with sufficient power. Single point scanning systems allow lower laser power to be used, but building up an image of flow in skin requires mechanical scanning of the laser, which results in a high image acquisition time, making the system awkward to use. A new approach developed here involves scanning a line along a target, and imaging the line with a 1D sensor array. This means that only one axis of mechanical scanning is required, reducing the scanning speed, and the laser power is vastly reduced from that required for a 2D system. This approach lends itself well to the use of integrated CMOS detectors, as the smaller pixel number means that a linear sensor array can be implemented on an IC which has integrated processing while keeping overall IC size, and hence cost, lower than equivalent 2D imaging systems. A number of front-end and processing circuits are investigated in terms of their suitability for this application. This is done by simulating a range of possible designs, including several logarithmic pixels, active pixel sensors and opamp-based linear front-ends. Where possible previously fabricated ICs using similar sensors were tested in a laser Doppler flowmetry system to verify simulation results. A first prototype IC (known as BVIPS1) implements a 64x1 array of buffered logarithmic pixels, chosen for their combination of sufficient gain and bandwidth and compact size. The IC makes use of the space available to include two front-end circuits per pixel, allowing other circuits to be prototyped. This allows a linear front-end based on opamps to be tested. It is found that both designs can detect changes in blood flow despite significant discrepancies between simulated and measured IC performance. However, the signal-noise ratio for flux readings is high, and the logarithmic pixel array suffers from high fixed pattern noise, and noise and distortion that makes vein location impossible. A second prototype IC (BVIPS2) consists of dual 64x1 arrays, and integrated processing. The sensor arrays are a logarithmic array, which addresses the problems of the first IC and uses alternative, individually selectable front-ends for each pixel to reduce fixed-pattern noise, and an array of opamp-based linear detectors. Simulation and initial testing is performed to show that this design operates as intended, and partially overcomes the problems found on the previous IC - the IC shows reduced fixed pattern noise and better spatial detection of blood flow changes, although there is still significant noise.

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Ghouth, Nahar Nizar A. "The assessment of pulpal blood flow using laser Doppler flowmetry." Thesis, University of Leeds, 2019. http://etheses.whiterose.ac.uk/22641/.

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Aims: The overall aim of this work was to study the use of Laser Doppler flowmetry for the assessment of the dental pulp in permanent teeth. The thesis is presented as four distinct studies; 1) A systematic review was carried out to assess the published evidence on the use of laser Doppler flowmetry in the assessment of the pulp status of permanent teeth, 2) A cross-sectional survey was carried out in order to understand the use of dental pulp tests by paediatric dentists and general dental practitioners in children with dental trauma in the United Kingdom, 3) The first clinical study aimed to assess whether laser Doppler flowmetry was more accurate than the conventional pulp sensibility tests (Electric pulp test and ethyl chloride) in assessing the pulp status of permanent anterior teeth in children, and 4) The second clinical study aimed to prospectively monitor pulp sensibility/vitality of traumatised teeth using laser Doppler flowmetry, electric pulp testing and ethyl chloride, and to prospectively investigate the accuracy of each test. Methods: Systematic review: A systematic literature search, using MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, www.clinicaltrials.gov and www.controlled-trials.com, in addition to citation and manual reference list searches, was conducted up to 15th January 2018. A risk of bias assessment was performed using the quality assessment for diagnostic accuracy studies tool (QUADAS-2) with all steps performed independently by two reviewers. Survey: A cross-sectional study utilising an 18-item questionnaire that was developed using the Bristol Online Survey (BOS) tool and circulated electronically to the members of the British Society of Paediatric Dentistry between June and August 2017. Clinical study 1: A cross-sectional cohort diagnostic accuracy study with randomisation was carried out in 8-16-year-old children. Participants had one maxillary central or lateral incisor with either a completed root canal treatment or pulp extirpation and a contra-lateral tooth with vital pulp. The outcome measures included the cut-off threshold for LDF and the sensitivity, specificity and predictive values as well as the repeatability of each test. The Receiver Operating Characteristic (ROC) curve and the contingency 2X2 table were used for analysis. Kappa scores were used to assess the repeatability of EPT and ethyl chloride while inter-class correlation was used for LDF. Clinical study 2: Children who sustained dental trauma to an anterior permanent tooth with uncertain pulp vitality requiring monitoring for a minimum of 12 months were included in the study. Recordings of dental pulp tests were carried out at baseline and at the end of the follow-up period. Results Systematic review: Only four studies all with a high risk of bias were included in the final systematic review for analysis. Laser Doppler flowmetry was reported to be more accurate in differentiating between teeth with normal pulps and pulp necrosis with a sensitivity of (81.8-100%) and specificity of 100 % in comparison to other vitality tests such as pulp oximetry (sensitivity = 81.3 %, specificity = 94.9 % ) and sensibility tests such as electric pulp testing (EPT) (sensitivity = 63.3 - 91.5 %, specificity = 88 - 100 %). Survey: One hundred and forty-one respondents, both, paediatric dental specialists (56%) and GDPs (44%) were included in the analysis. Almost all specialists (93.7%) reported using sensibility tests routinely in comparison to 80.6% of GDPs. Child perception and cooperation were the most commonly reported barriers. GDPs mainly used cold testing, while specialists used cold and electric pulp tests equally. Inconsistencies in recording as well as documentation the results varied among respondents. Only a few specialists reported having some experience in using laser Doppler flowmetry. Clinical study 1: There was a significant difference between the Flux values for teeth with vital and non-vital pulps. The best cut-off ratio for LDF was 0.6 yielding a sensitivity of 54 % and a specificity of 32 % which were lower than the values of electric pulp test (Sensitivity = 83.8 - 94.6 %, Specificity = 89.2 - 97.6 %) and ethyl chloride (Sensitivity = 81.1 - 91.9 %, Specificity = 73 - 81.1 %). The repeatability of LDF, EPT and ethyl chloride were 0.85, 0.86 and 0.81, respectively. Clinical study 2: The study included a convenience sample size of 15 participants with a mean age of 10.7 years (SD=1.66), age range 8-14 years. The mean follow-up period was 7.29 months (SD 1.9) with a range of 6-12 months. All traumatised teeth remained vital at the end of follow-up except one tooth. The specificity of LDF at baseline was 80% compared to 66.6% and 60-73.3% for EPT and ethyl chloride, respectively. At the end of the follow-up period, LDF showed lower specificity (71.4 %) than EPT (78.5 - 85.7 %) and ethyl chloride (71.4 - 78.5 %). Conclusion: Despite the high reported sensitivity and specificity of laser Doppler flowmetry in the systematic review, these data were found to be based on studies with a high level of bias and serious shortfalls in study designs. The survey of specialists and GDP's showed that the use of pulp sensibility tests was relatively high amongst respondents while those of vitality tests were very low. Barriers and inconsistencies in the technique and recording of the results of sensibility tests were evident. The frequency and timing of using sensibility tests in line with international guidelines were stressed. The use of standardised techniques involving methods considered to improve reliability was highlighted. The results of the clinical studies showed that there was a high probability of false results when using LDF in assessing the pulp blood flow/pulp vitality. LDF was unable to differentiate between teeth with vital and non-vital pulps in children between the ages of 8-16 years with an acceptable level of confidence in the first clinical study. Within the limitations of the second clinical study, LDF showed better specificity than both EPT and ethyl chloride in predicting the outcome of the pulp at baseline but less at the end of follow-up. Due to the small sample size and relatively short follow-up period, the results of the second clinical study have been interpreted with caution. Therefore, the published data on the accuracy of LDF can not be accepted as they are based on studies with unacceptable flaws in study design. Our studies have shown that not only the use of LDF or even the experience of clinicians with its use is extremely low, but also its specificity and sensitivity were of a level which is unacceptable for recommending its meaningful clinical use.

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Hinsdale, Taylor A. "Laser Speckle Imaging: A Quantitative Tool for Flow Analysis." DigitalCommons@CalPoly, 2014. https://digitalcommons.calpoly.edu/theses/1251.

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Laser speckle imaging, often referred to as laser speckle contrast analysis (LASCA), has been sought after as a quasi-real-time, full-field, flow visualization method. It has been proven to be a valid and reliable qualitative method, but there has yet to be any definitive consensus on its ability to be used as a quantitative tool. The biggest impediment to the process of quantifying speckle measurements is the introduction of additional non dynamic speckle patterns from the surroundings. The dynamic speckle pattern under investigation is often obscured by noise caused by background static speckle patterns. One proposed solution to this problem is known as dynamic laser speckle imaging (dLSI). dLSI attempts to isolate the dynamic speckle signal from the previously mentioned background and provide a consistent dynamic measurement. This paper will investigate the use of this method over a range of experimental and simulated conditions. While it is believable that dLSI could be used quantitatively, there were inconsistencies that arose during analysis. Simulated data showed that if the mixed dynamic and static speckle patterns were modeled as the sum of two independent speckle patterns, increasing static contributions led to decreasing dynamic contrast contributions, something not expected by theory. Experimentation also showed that there were scenarios where scattering from the dynamic media obscured scattering from the static medium, resulting in poor estimates of the velocities causing the dynamic scattering. In light of these observations, steps were proposed and outlined to further investigate into this method. With more research it should be possible to create a set of conditions where dLSI is known be accurate and quantitative.

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12

Gu, Quan. "An analogue integrated circuit design for laser doppler blood flow measurement." Thesis, University of Nottingham, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.580293.

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Laser Doppler Blood Flowmetry (LDF) is a non-invasive optical technique for measuring microvascular blood flow in tissue. The existing LDF systems employ either scanning techniques or full-field imaging using discrete devices and digital signal processing units. However, the scanning systems have limitations in their imaging speed. The discrete full-field systems are not compact and at present are limited in bandwidth. This work deals with the design of a LDF system implemented with analogue integrated circuits. The design starts with the investigation of the design of each building block required in a single pixel and ends with a 4 x 4 pixel array. Four batches of chips were designed and fabricated with a standard O.35/Lm CMOS process. The first three chips were dedicated to the optimisation of the building blocks at the pixel level, and the fourth chip was dedicated to the 4 x 4 pixel array. In each pixel, the building blocks include a front-end, a bandpass filter and a frequency weighted filter. The optimisation of the front-end is based on the comparison between the simple front-end, feedback front-end and regulated cascode (RGC) front-end. The choice of the type of the bandpass filter and the frequency weighted filter is based on the comparison between the switched capacitor (SC), the operational transconductance capacitor (OTA-C) and the hysteretic differentiator (HD) circuits. With the analysis, simulation and measurement of the different techniques, the RGC front-end, OTA-C HD bandpass filter and OTA-C frequency weighted filter were used in the 4 x 4 pixel array as the result of the trade-offs between multiple design parameters. The 4 x 4 pixel array was used to measure the blood concentration and flow on the human finger. With the aid of the off-chip digital squaring and averaging, the change of blood concentration and flow before and after finger occlusion was clearly observed in both frequency and time domains. With the ultimate aim of scaling the 4 x 4 pixel array to a larger size, the system speed, price and size were estimated and compared with existing LDF systems. The analogue integrated circuit LDF system shows good trade-offs between speed, price and size and has the potential to cost-effectively fabricate a 64 x 64 array.

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Hwang, Suk-Moon. "Assessments of blood flow in portwine stains by laser Doppler flowmetry." Thesis, University of Strathclyde, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366897.

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14

Valdés, Escobar Claudia Patricia. "New laser speckle methods for in vivo blood flow imaging and monitoring." Doctoral thesis, Universitat Politècnica de Catalunya, 2014. http://hdl.handle.net/10803/285015.

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Blood flow and its regulation, as well as hemodynamics in general, are important for the health of tissues and hence the measurement of these quantities has many applications in research and clinical environments. Various optical techniques are attractive for the measurement of blood flow since they are often non- or minimally-invasive, continuous and are relatively inexpensive. During my PhD I have contributed to the monitoring of blood flow in experimental animal models with the construction of a multimodal device, based on laser speckle flowmetry and optical intrinsic signals, capable of measuring superficial microvascular cerebral blood flow, blood oxygenation and blood volume for translational research. This device was applied in animal models of ischemic stroke and is flexible to be modified and used for other purposes. In doing so, I have developed new experimental methods and image processing protocols that allowed us to perform longitudinal studies where the animal can be removed from the device several times. Furthermore, this device has been used as a tool in a multi-disciplinary study to understand the role of the Mannose-binding lectin protein in reperfusion injury after an ischemic stroke in animal models. This then led to the main contribution of this work which is the development of the speckle contrast optical spectroscopy and tomography, a new non-invasive, optical technique for deep blood flow measurement that paves the way for deeper and three dimensional imaging of blood flow. This new method was first developed from a theoretical perspective. Then it was validated in tissue simulating phantoms and demonstrated to be feasible in measurements on the human arm muscle. Overall, these contributions will allow the development of cost-effective, non-invasive tomographic methods for the measurement of blood flow even in humans.
El flujo sanguíneo y su regulación, así como la hemodinámica en general, son parámetros importantes para determinar el estado de salud de los tejidos; por esto, su medición tiene numerosas aplicaciones en los ámbitos clínico y de investigación. Varias técnicas ópticas resultan atractivas para la medición del flujo sanguíneo dado su carácter no invasivo o mínimamente invasivo, continuo y relativamente económico. Durante mi trabajo doctoral he contribuido a la monitorización del flujo sanguíneo, en modelos de experimentación animal, con la construcción de un dispositivo multimodo, basado en la flujometría de speckle láser (laser speckle flowmetry, LSF) y las señales ópticas intrínsicas (optical intrinsic signals, OIS), capaz de medir flujo sanguíneo de la microvasculatura superficial en el cerebro, oxigenación sanguínea y volumen sanguíneo en investigación traslacional. Este dispositivo fue aplicado en modelos animales de infarto cerebral; sin embargo, es flexible y puede ser modificado y utilizado para otros propósitos. Así pues, he desarrollado nuevos métodos experimentales y protocolos de procesamiento de imágenes que nos permitieron llevar a cabo estudios longitudinales, donde los animales pueden ser removidos del dispositivo en repetidas ocasiones. Adicionalmente, este dispositivo fue utilizado como herramienta en un estudio multidisciplinario para entender el papel de la proteína lectina de unión a la manosa (MBL) en las lesiones por isquemia-reperfusión después de un infarto cerebral en modelos animales. Este estudio, dio origen a la mayor contribución de este trabajo, siendo esta el desarrollo de la espectroscopía y tomografía óptica de contraste de speckle; una novedosa técnica óptica, no invasiva para medición de flujo sanguíneo profundo que allana el camino para la obtención de imágenes tridimensionales de flujo sanguíneo más profundo. Este nuevo método, se desarrolló primero desde una perspectiva teórica, y posteriormente se validó en phantoms de tejido biológico, demostrando su factibilidad en mediciones realizadas en el músculo del antebrazo de un paciente. En general, estas contribuciones permitirán el desarrollo de métodos tomográficos, no invasivos y rentables para la medición de flujo sanguíneo, extensibles incluso a seres humanos

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Obeid, A. N. "The measurement of blood flow in the microcirculation using Laser Doppler Flowmetry." Thesis, Oxford Brookes University, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235228.

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Valdés, Escobar Claudia Patricia. "New laser speckle methods for in vivo blood flow imaging and monitoring." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM4367/document.

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Le débit sanguin et sa régulation sont des indicateurs importants de la santé des tissus. Leur mesure a de nombreuses applications en recherche fondamentale et clinique. Certaines techniques optiques constituent un moyen intéressant pour la mesure du débit sanguin, car en général elles sont peu invasives et relativement abordables car elles utilisent des systèmes d'illumination continus. Pendant ma thèse, j'ai contribué au développement de techniques de suivi de la circulation sanguine dans des modèles animaux avec la construction d'un dispositif multimodal basé sur la fluxmétrie laser et sur l'imagerie des signaux optiques intrinsèques, capable de mesurer les paramètre hémodynamiques microvasculaire au niveau superficiel du cerveau. Ce dispositif, testé sur des modèles animaux d'accident vasculaire cérébral, est adaptable et peut être utilisé à d'autres fins. En parallèle, j'ai mis au point des nouvelles méthodes expérimentales et des protocoles de traitement d'images qui ont permis de réaliser des études longitudinales. En outre, ce dispositif a été utilisé dans une étude multidisciplinaire pour comprendre le rôle d'une protéine impliquée dans le cas de lésions de reperfusion après un accident vasculaire cérébral ischémique dans des modèles animaux. Ma contribution majeure réside dans le développement de l'imagerie de contraste de speckle spectroscopique et tomographique, nouvelle technique d'imagerie 3D non invasive pour la mesure du débit sanguin en profondeur. Dans l'ensemble, ces contributions permettront le développement de méthodes tomographiques non invasives rentables pour la mesure du débit sanguin chez l'homme
Blood flow and its regulation are important for the health of tissues and its measurement has many applications in research and clinical environments. Optical techniques are often attractive for the non- or minimally-invasive, continuous and relatively inexpensive measurement of blood flow. This work contributes to the monitoring of blood flow in translational research with the construction of a multimodal device, based on laser speckle flowmetry and optical intrinsic signals, capable of measuring superficial microvascular cerebral blood flow, blood oxygenation and blood volume. This device was applied in animal models of ischemic stroke and is flexible to be modified and used for other purposes. In doing so, I have developed new experimental methods and image processing protocols that allowed us to perform longitudinal studies where the animal can be removed from the device several times. This device has also been used to elucidate the role of the Mannose-binding lectin protein in reperfusion injury after an ischemic stroke in animal models. This led to the main contribution of this work: the development of the speckle contrast optical spectroscopy and tomography, a new non-invasive, optical technique for deep blood flow measurement that paves the way for deeper and three dimensional imaging of blood flow. This new method was first developed from a theoretical perspective. Then it was validated in tissue simulating phantoms and demonstrated to be feasible in measurements on the human arm muscle. Overall, these contributions will allow the development of cost-effective, non-invasive tomographic methods for the measurement of blood flow even in humans

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17

Ward, Geoffrey. "Laser Doppler flowmetry : theoretical and in vitro models with red and green lasers." Thesis, Oxford Brookes University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318536.

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18

Barnett, Nicholas James. "The development of biomedical instrumentation using backscattered laser light." Thesis, Oxford Brookes University, 1990. https://radar.brookes.ac.uk/radar/items/854b71a4-e72a-4396-bac2-df2608345d2d/1.

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This thesis is concerned with the measurement of blood flow and oxygen saturation in the microcirculation using the techniques of laser Doppler flowmetry and pulse oximetry. An investigation of the responses of Doppler flowmeters using different signal processing bandwidths and laser sources revealed two major findings. Firstly, that careful choice of processing bandwidth is required in order to sample the whole range of possible Doppler frequencies present in the backscattered light. Secondly, that the choice of laser source is important in governing the output stability of a flowmeter. Another investigation focused on the evaluation of a dual channel laser Doppler flowmeter using both in vitro and in vivo models. It was demonstrated that the instrument permitted a useful method of obtaining flow information by comparing simultaneous responses at experimental and control sites. The choice of laser wavelength was investigated in a study to determine whether blood flow measurements are obtained from different depths within the skin tissue. The results indicate that some depth discrimination is obtainable using instruments operating at different wavelengths, however it is difficult to demonstrate the effect in vivo. In a separate study it was shown that pressure applied to the skin surface greatly affects the underlying blood flow. It is recommended that care has to be taken when positioning Doppler probes on the skin. A reflection pulse oximeter was developed using laser light backscattered from the skin. The instrument was evaluated in vitro and in vivo by comparing desaturation responses with a commercial transmission pulse oximeter. The reflection oximeter was demonstrated to reliably follow trends in oxygen saturation but several problems prevented instrument calibration. Finally, a device combining laser Doppler flowmetry with reflection pulse oximetry was developed and used in vivo to follow trends in blood flow and oxygen saturation from the same tissue sample.

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CORREA, MELISSA S. F. "Estudo das origens e funções do fluxo sanguíneo medido em dentes humanos usando a fluxometria laser Doppler." reponame:Repositório Institucional do IPEN, 2006. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11703.

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Made available in DSpace on 2014-10-09T12:54:56Z (GMT). No. of bitstreams: 0
Made available in DSpace on 2014-10-09T14:06:59Z (GMT). No. of bitstreams: 1 12801.pdf: 2431192 bytes, checksum: b7e93ba00685003bca6016af5031d88f (MD5)
Dissertação (Mestrado Profissionalizante em Lasers em Odontologia)
IPEN/D-MPLO
Instituto de Pesquisas Energéticas e Nucleares - IPEN/CNEN-SP; Faculdade de Odontologia, Universidade de São Paulo, São Paulo

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20

Kumar, Hemant. "Software analytical tool for assessing cardiac blood flow parameters /." View thesis, 2001. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030724.122149/index.html.

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21

Golster, Helena. "Regulation of microvascular blood flow : a clinical and experimental study based on laser Doppler perfusion imaging /." Linköping : Univ, 2001. http://www.bibl.liu.se/liupubl/disp/disp2001/med683s.pdf.

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22

Essex, Timothy John Hudson. "The development and evaluation of a scanning laser Doppler instrument for imaging skin blood flow." Thesis, Northumbria University, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357147.

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23

Cobb, Jonathan E. "An in-shoe laser Doppler sensor for assessing plantar blood flow in the diabetic foot." Thesis, Bournemouth University, 2000. http://eprints.bournemouth.ac.uk/312/.

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An in-shoe laser Doppler sensor for assessing plantar blood flow in the diabetic foot. Jonathan Edwin Cobb Plantar ulceration is a complication of the diabetic foot prevalent in adults with type 11 diabetes mellitus. Although neuropathy, microvascular disease and biornechanical factors are all implicated, the mechanism by which the tissue becomes pre-disposed to damage remains unclear. Recent theories suggest that the nutritional supply to the tissue is compromised, either by increased flow through the arteriovenous anastomoses ('capillary steal' theory) or through changes in the micro vascu I ature (haemodynamic hypothesis). Clinical data to support these ideas has been limited to assessment of the unclad foot under rest conditions. A limitation of previous studies has been the exclusion of static and dynamic tissue loading, despite extensive evidence that these biornechanical factors are essential in the development of plantar ulceration. The present study has overcome these problems by allowing assessment of plantar blood flow, in-shoe, during standing and walking. The system comprises a laser Doppler blood flux sensor operating at 780nm, load sensor, measurement shoe, instrumentation, and analysis software. In-vitro calibration was performed using standard techniques. An in-vivo study of a small group of diabetic subjects indicated differences in the blood flux response between diabetic neuropaths, diabetics with vascular complications and a control group. For example, following a loading period of 120s, relative increases in response from rest to peak were: Control (150% to 259%), Vascular (-70% to 242%), Neuropathic (109%-174%) and recovery times to 50% of the peak response were: Control (33s to 45s), Vascular (43s to >120s), Neuropathic (>120s). Dynamic re-perfusion rates (arbitrary units per millisecond) obtained for the swing phase of gait were: Control (6.1 a. u/ms to 7.9 a. u/ms), Vascular (4 a. u/ms to 6.2 a. u/ms), Neuropathic (2.3 a. u/ms to 4.5 a. u/ms).

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24

Young, Anthony M. "Investigation of Laser Speckle Contrast Imaging's Sensitivity to Flow." Miami University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=miami153256524246362.

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25

Kumazawa, Takao, Shigeyuki Suzuki, Jun Sato, Tomoko Koeda, and Yoichiro Tsujii. "Sympathetically induced paradoxical increases of the cutaneous blood flow in chronically inflamed rats." Thesis, Elsevier, 1996. http://hdl.handle.net/2237/16719.

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26

Rotenberg, Shaun. "Blood Flow, Tissue Thickness, and Molecular Changes during Connective Tissue Graft Early Healing." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1273335634.

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27

Martin, Denis James. "An investigation into the effects of low level laser therapy on arterial blood flow in skeletal muscle." Thesis, University of Ulster, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385613.

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28

Clements, B. Alyson. "Low intensity laser therapy (LILT) and combined phototherapy/LILT : effects upon blood flow and wound healing in humans." Thesis, University of Ulster, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241724.

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Corrêa, Melissa Santos Folgosi. "Caracterização das flutuações do sinal laser doppller do fluxo microvascular." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/85/85134/tde-31102011-102809/.

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O sinal de fluxo cutâneo obtido via fluxometria Laser Doppler (SFLD) tem flutuações de baixas frequências que estão relacionadas a mecanismos de controle do fluxo microvascular. Análises espectrais, via transformada de Fourier e transformada de wavelet, têm sido usadas para correlacionar as flutuações de SFLD com os seguintes mecanismos de controle de fluxo: metabólico, metabólico NO-dependente, neurogênico e miogênico, nos respectivos intervalos de frequência 0,005-0,0095 Hz, 0,0095-0,02 Hz, 0,02-0,05 Hz e 0,05-0,15 Hz. A potência do sinal, em cada intervalo de frequência, geralmente é usada como uma medida da atividade do mecanismo de controle microvascular relacionado. Uma vez que os métodos usados de análise são espectrais, as características das flutuações do SFLD, em cada intervalo de frequência, no domínio do tempo são desconhecidas. Como consequência, há ausência de critérios objetivos para medir adequadamente, em cada intervalo de frequência, os parâmetros hemodinâmicos relacionados. O objetivo deste trabalho foi caracterizar e quantificar flutuações temporais, espaciais e espaço-temporais do SFLD em cada faixa de frequência, usando um método no domínio do tempo. Os fluxos basais (320C) e termicamente estimulados à (420C) das regiões volares de antebraços de 20 voluntários saudáveis foram coletados em duas regiões próximas e analisados. As análises dos dados obtidos indicam que janelas temporais pequenas (1 minuto) são aceitáveis para a quantificação do fluxo médio, e que janelas temporais maiores são necessários para quantificar as flutuações de fluxo. A análise espaço-temporal revelou uma forte correlação entre sinais (em todas as bandas, exceto na banda B5) das duas regiões investigadas, durante longos intervalos de tempo, quando as duas regiões estudadas foram termicamente estimuladas, e menor variabilidade intragrupo quando comparada à obtida para os valores médios das flutuações, sugerindo que o intervalo de tempo de correlação é um parâmetro promissor para estudar mecanismos de controle do fluxo microvascular.
The laser Doppler flow signal from the skin (LDFS) has low-frequency fluctuations which are related to microvascular mechanisms of flow control. The Fourier and the wavelet spectral analysis has been used to correlate fluctuations in the LDFS with the metabolic, metabolic NO-dependent, neurogenic and myogenic mechanisms of control in the frequency intervals 0.005-0.0095 Hz, 0.0095-0.02 Hz, 0.02-0.05 Hz and 0.05-0.15 Hz, respectively. The signal power, in each frequency interval, is generally used as a measure of the activity of the related mechanism of microvascular control. Since spectral analysis methods have been used, the time-domain characteristics of the fluctuations in the LDFS in each frequency interval are unknown. As a consequence, there is a lack of objective criteria to properly measure, in each frequency interval, the related hemodynamic parameters. The aim of this work was characterizing and quantifying temporal, spatial and spatial-temporal fluctuations in the LDFS in each frequency band, using a time-domain method. Baseline (320C) and thermally stimulated (420C) LDFS of volar forearms from 20 healthy volunteers were collected from two close regions and analyzed. The data obtained indicate that short-time windows (1 minute) are acceptable for quantifying the mean flow, and that larger time-windows are needed for quantifying the flow fluctuations. The spatialtemporal analysis revealed strong correlations between signals (all bands, except B5) from the two investigated regions, during large time intervals when thermally stimulated, and lower intragroup variability than the ones obtained for the mean values of fluctuations, suggesting that the time interval of correlation is a promising parameter for studying mechanisms of microvascular flow control.

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Rayanne, Pinto Costa. "Transition to turbulence within an eccentric stenosis geometry under steady flow using laser Doppler vibrometry for a non-Newtonian and Newtonian fluid." University of Akron / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=akron1605627935635379.

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31

Vesperini, Doriane. "Biomechanical study of cells in microfluidic flow : application to sorting and platelet production." Thesis, Compiègne, 2018. http://www.theses.fr/2018COMP2437/document.

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Les mégacaryocytes sont des cellules de la moelle osseuse, à l’origine de la production des plaquettes sanguines. Quand elles arrivent à maturité, elles grossissent et émettent des prolongements de cytoplasme à travers la paroi des vaisseaux irriguant la moelle. Dans la circulation sanguine, ces prolongements, soumis aux forces de l’écoulement, s’allongent et se rompent pour former des plaquettes. Des techniques microfluidiques capables de produire des plaquettes in vitro existent et sont une alternative prometteuse au don. Mais le rendement reste à améliorer. Pour cela, il est nécessaire de mieux comprendre la fragmentation des mégacaryocytes en plaquettes. Ce travail de doctorat s’inscrit dans ce contexte et sera développé en deux axes principaux dans ce manuscrit. Dans une première partie nous développons une méthode pour trier des cellules en fonction de leur déformabilité, afin de savoir si les propriétés mécaniques d’un mégacaryocyte sont liées à leur stade de maturité. La méthode a d’abord été mise au point avec des microcapsules. Leurs propriétés mécaniques sont déterminées par analyse inverse à partir de la mesure de leur forme en écoulement dans des constrictions droites. Puis le dispositif utilisé a été miniaturisé pour s’adapter à la taille des cellules. Pour la caractérisation de leurs propriétés mécaniques, deux outils ont été utilisés: l’analyse inverse et la microscopie à force atomique sans pointe. Une deuxième partie porte sur l’étude de l’élongation et de la rupture de mégacaryocytes soumis écoulement. Nous avons quantifié les variations spatiotemporelles du taux d’élongation et développé un protocole d’ablation laser pour étudier les mécanismes de rupture de cellules en élongation
When they mature in the bone marrow, the precursors of platelets, called megakaryocytes, grow and extend protrusions able to join blood circulation. There these protrusions elongate and break into platelets. Microfluidic techniques for in vitro platelet production represent a promising alternative to donation. In order to enhance platelet production and match the needs of clinical applications such as transfusion, we need to better understand the fragmentation of megakaryocytes into platelets. Our contribution will be described in this manuscript in two main axes. First, in order to know if mechanical properties of megakaryocytes can indicate their maturity stage, we develop a cell sorting method based on deformability. The method is first validated with microcapsules. Their mechanical properties are determined by inverse analysis from their shape under flow in straight microchannels. Then the device is downscaled. The characterization of cell mechanical properties are performed using inverse analysis and tipless atomic force microscopy. Second, we study megakaryocyte elongation and rupture in a microfluidic device. We quantify the spatial and temporal variations of the elongation rate and develop a laser ablation protocol to trigger and study the rupture of elongating cells

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32

Bergstrand, Sara. "Tissue Blood Flow Responses to External Pressure Using LDF and PPG : Testing a System Developed for Pressure Ulcer Research." Licentiate thesis, Linköping : Department of Medical and Health Sciences, Linköping University, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-51886.

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33

Khalil, Adil. "Processing of laser speckle contrast images : study of mathematical models and use of nonlinear analyses to investigate the impact of aging on microvascular blood flow." Thesis, Angers, 2017. http://www.theses.fr/2017ANGE0006/document.

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Le vieillissement est un facteur de risque des maladies cardiovasculaires. Il est associé à des altérations fonctionnelles et structurelles du système vasculaire.Une étude approfondie du processus de vieillissement et le développement de systèmes d’imagerie et des traitements de données associés deviennent donc une priorité. Par l’analyse d’images de contraste par speckle laser (LSCI), l’objectif de cette thèse est d’étudier l’influence de l’âge sur la micro circulation.Pour ce faire, des données de LSCI ont été acquises sur l’avant-bras de sujets sains jeunes et âgés. A partir de modèles mathématiques, nous avons déterminé la vitesse des érythrocytes de la micro circulation chez les deux groupes de sujets. Par ailleurs, nous avons également mené une étude de la complexité de séries temporelles d’ LSCI s’appuyant sur des mesures d’entropie multi échelle. Nos résultats montrent que : 1) le groupe de sujets plus âgés présente des valeurs de vitesse des globules rouges significativement plus élevées que celles des sujets jeunes à l’hyperémie réactive post-occlusive; 2) les fluctuations des séries temporelles de LSCI dans le groupe des sujets jeunes ont une complexité supérieure à celles du groupe de sujets âgés. Ces modifications observées sur la micro circulation pourraient être attribuées à des modifications du système vasculaire dans son ensemble. La compréhension de ces altérations pourrait conduire à de nouvelles perspectives en matière de prévention et de traitement des pathologies liées à l’âge
Aging is a primary risk factor for cardiovascular diseases. It is associated with functional and structural alterations in the vascular system. Therefore, a deep study of the aging process and the development of imaging systems and associated processing become of the utmost importance. By processing laser speckle contrast images (LSCI), this PhD work aims at studying the influence of age on microcirculation. In our work, LSCI data were acquired from the skin forearm of healthy subjects, subdivided into two age groups (younger and older). From mathematical models, we determined red blood cells velocity in microcirculation in the two groups of subjects. Moreover, we applied multiscale entropy-based algorithms to LSCI time series in order to study the complexity of microvascular signals. Our main findings are: 1) the older group has significantly higher velocity values than the younger group at post-occlusive reactive hyperaemia; 2) LSCI fluctuations in the younger group have significantly higher complexity than those of the older group. Age-related changes in skin microcirculation can be attributed to alterations in the vascular system as a whole. Understanding these changes in the microcirculatory system may give new insights for prevention and treatment of age-related diseases

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Soleimanzad, Haleh. "Development and application of quantitative imaging to study cerebral blood flow in a mouse model of obesity." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS604.

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Selon l’organisation mondiale de la santé, dans les pays en développement, la proportion de personnes obèses a presque triplé depuis 1980 et presque doublé dans les pays à revenu élevé. Parmi ces statistiques, en France, 16,8% des hommes et 17,4% des femmes sont obèses. Les taux mondiaux d'obésité devraient monter au cours de la prochaine décennie pour atteindre un cinquième des adultes en 2025. L'obésité est due à de multiples facteurs, dont la consommation excessive d’aliments riches en gras et en sucres, ainsi que des facteurs génétiques, psychosociaux et environnementaux. L'incidence de maladies telles que le cancer, le diabète et les maladies cardiovasculaires est supérieure chez les personnes obèses. L’obésité a également un impact néfaste sur le fonctionnement du cerveau, ce qui entraîne davantage d’accidents vasculaires cérébraux et des maladies neurodégénératives chez les personnes obèses. Une activité cérébrale normale impose des besoins énergétiques dynamiques qui sont satisfaits par le flux sanguin cérébral (Cerebral Blood Flow, CBF). La perfusion adéquate des tissus cérébraux au bon moment et au bon endroit parmi les quelques 160 milliards de cellules qui composent le cerveau adulte humain est vital. Malgré des données obtenues sur des tranches de cerveau concernant les problèmes de barrière hémato-encéphalique chez les personnes obèses, le devenir du CBF au cours de l'obésité n'a pas encore été étudié. Une des raisons à cela est la difficulté à enregistrer le CBF in vivo et de le suivre dans le temps, pendant une activation cérébrale et sur une large échelle avec une résolution spatio-temporelle appropriée. Afin d'évaluer l'influence de l'obésité sur le CBF, au repos et pendant la stimulation sensorielle, nous avons développé une technique optique appelée l'imagerie de contraste laser par exposition multiple (MESI). La technique repose sur le calcul du contraste de speckle, qui est lié à la vitesse des diffuseurs (globules rouges). Il permet une imagerie superficielle à large champ des variations relatives de flux sanguin dans le cortex de la souris. Nous avons caractérisé les performances du système en utilisant des fantômes microfluidiques. L’acquisition du contraste pour différentes durées d’exposition permet de discriminer les diffuseurs statiques et dynamiques (en mouvement) et donc d’obtenir une image quantitative des variations du CBF. Nous avons étudié l'activation cérébrale en utilisant la stimulation olfactive par des flux d'odeurs contrôlés présentés à la souris anesthésiée. Le bulbe olfactif est une structure sensorielle essentielle des mammifères pour le codage des odeurs et il est bien adapté à l'imagerie optique car l’activité neuronale et vasculaire est détectée dans les régions superficielles de cette structure. Nous avons observé une diminution significative du CBF évoqué par stimulation odorante chez les souris obèses (sous régime hyperlipidique) par rapport aux souris témoins (sous régime standard). Chez les souris contrôles, les variations de CBF sont élevées dans les vaisseaux sanguins de grand diamètre et diminuent dans les vaisseaux sanguins de petit calibre. Cette variation dépendant du diamètre est perdue chez les souris obèses qui présentent même un CBF significativement réduit au repos, au cours d'une activité vasculaire spontanée. De plus, afin de mieux comprendre la morphologie du système vasculaire, nous avons commencé l’étude par iDISCO de la densité et la distribution des vaisseaux dans l’ensemble du cerveau in vitro chez des souris obèses comparées aux contrôles. En conclusion, les résultats obtenus sur le CBF chez les souris obèses par la mise au point d’une technique d’imagerie optique à large champ MESI, indiquent que l'obésité impacte le fonctionnement vasculaire en dérégulant le débit sanguin cérébral
Obesity is a global health threat. Since 1980 the proportion of obese or overweight individuals tripled in developing countries and doubled in high-income countries. In France 16.8% of men and 17.4% of women are obese. In the actual tendency persists, one-fifth of adults worldwide will be obese by 2025. Obesity is characterized by exaggerated weight gain and accumulation of fat tissue and is due to multiple factors including excessive consumption of high fat-sweet food and genetic, psychosocial and environmental factors. It is linked to an increase in the incidence of diseases such as cancer, diabetes and cardiovascular disease. Obesity has also a detrimental impact on brain function leading to higher rate of stroke and neurodegenerative diseases. Normal brain activity imposes dynamic energy requirements. Energy needs are fulfilled by Cerebral Blood Flow (CBF) to perfuse the brain tissue at the right time and the right place among the hundred of billons of cells that compose the human adult brain. Although dysfunction of the blood brain barrier was observed in brain slices, the fate of CBF during obesity in vivo is unknown. One reason for this is the difficulty to record CBF over time in vivo and to follow the time course of activation of large populations of cells with an appropriate spatiotemporal resolution. In order to evaluate the influence of obesity on CBF, at rest and during sensory stimulation, we have developed an optical technique termed multi-exposure speckle contrast imaging (MESI). In the last years, MESI has been validated for imaging relative changes in CBF at the surface of the rodent brain in vivo, the standard mammalian model for brain studies. The technique relies on the calculation of the spatial speckle contrast, which is related to the velocity of scatterers (red blood cells), and allows wide-field imaging of CBF at the mesoscopic level. We have characterized the performances of the system using microfluidic phantoms. We further demonstrated the ability of our MESI system to discriminate the moving and static diffusers contribution and therefore to provide accurate estimate of CBF changes in vivo. The olfactory bulb is a major hub for the processing of olfactory information in the brain of all mammals. It is well suited for optical imaging of brain activation since neuronal and vascular activities are detected very superficial at the surface of this structure. Using MESI, we have studied brain activation in control and obese mice. We have performed olfactory activation by delivering controlled odorants fluxes to anesthetized mice. We observed a significant decrease in odor-evoked CBF with a loss of diameter-dependent regulation of CBF in obese mice (high fat diet) compared to control lean mice (standard diet). We showed that CBF regulation was lost in obese mice even at rest without any stimulation. Furthermore, to gain insights into the morphology of the vascular network, we started the study of the vessels density and distribution in the entire brain using an in vitro iDISCO approach in obese mice compared to control mice. Overall, these findings indicate that obesity can adversely affect CBF at rest and in response to neuronal activation in vivo

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Sjölund, Fanny. "Förhållandet mellan hudblodflöde och fysisk aktivitet." Thesis, Örebro universitet, Hälsoakademin, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-15834.

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SAMMAMFATTNING Bakgrund: Reaktiv hyperemi definieras som ett övergående ökat blodflöde över det normala efter en tids ischemi. Det kan registreras med laserdopplerteknik. Att registrera reaktiv hyperemi är ett sätt att värdera mikrocirkulationen. Det finns många flödesvariabler att studera varav tid till maxflöde efter ocklusion är en. Det har gjorts studier som undersöker om det finns ett samband mellan reaktiv hyperemi och fysisk aktivitet/syreupptagningsförmåga. Det har inte gjorts någon studie som undersöker tid till maxflöde och fysisk aktivitet. Syftet var att undersöka om det finns ett samband mellan fysisk aktivitet och reaktiv hyperemi med avseende på tid till maxflöde. Material och metod: Testpersoner fick bära en accelerometer en vecka under dygnets alla vakna timmar samt göra en registrering av reaktiv hyperemi med laserdoppler. För statistiska beräkningar användes oparat T-test för att undersöka skillnad mellan olika grad av fysisk aktivitet och tid till maxflöde. Resultat: Ingen statistiskt signifikant skillnad mellan olika aktivitetsgrad och tid till maxflöde kunde observeras. Slutsats: Den här studien visade inte på statistiskt signifikant samband mellan blodflöde och fysisk aktivitet.

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36

Atuma, Christer. "Gastrointestinal mucosal protective mechanisms : Mudolatory effects of Heliobacter pyroli on the gastric mucus gel barrier and mucosal blood flow in vivo." Doctoral thesis, Uppsala University, Department of Physiology, 2000. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1258.

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The gastrointestinal mucus gel layer and blood flow are two important mechanisms for protection at the pre-epithelial and sub-epithelial levels, respectively. Helicobacter pylori might circumvent these mechanisms and elicit a chronic inflammatory response with consequent ulcers in the stomach and duodenum. In this thesis, the physical state and properties of the adherent mucus gel layer was studied from the stomach to colon. Furthermore, the acute and chronic effects of H. pylori on the integrity of the mucus gel layer and mucosal blood flow were studied in the anesthetized rat.

A translucent mucus gel covers all studied segments of the gastrointestinal tract during fasting conditions, with the thickest layers in the colon and ileum. Carefully applied suction revealed that the mucus gel was a multi-layered structure comprising a firmly adherent layer covering the mucosa, impossible to remove, and a loosely adherent upper layer. The firmly adherent layer was thick and continuous in the corpus (80μm), antrum (154μm) and colon (116μm), but thin (<20μm) and discontinuous in the small intestine.

Following mucus removal, a rapid renewal of the loosely adherent layer ensued. The highest rate was observed in the colon with intermediate values in the small intestine. Mucus renewal in the stomach was attenuated on acute luminal application of water extracts from H. pylori (HPE). In animals with a chronic H. pylori infection the mucus renewal rate was unaffected, but the total gastric mucus gel thickness was reduced and the mucus secretory response to luminal acid (pH1) attenuated in the antrum.

HPE from type I strains acutely reduced corporal mucosal blood flow, measured with laser-Doppler flowmetry, by approximately 15%. The reduction in blood flow was mediated by a heat stable factor other than VacA and CagA. Inhibition of endogenous nitric oxide production with Nω-nitro-l-arginine augmented the decrease. However, ketotifen, a mast cell stabilizer, completely attenuated the effect of the extract as did the platelet activating factor (PAF) receptor-antagonist, WEB2086, thus depicting a detrimental role for the microvascular actions of PAF.

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37

Chen, Miao. "Endothelial Cell-Specific Knockout of Meis1 Protects Ischemic Hindlimb Through Vascular Remodeling." Diss., Virginia Tech, 2018. http://hdl.handle.net/10919/96188.

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Peripheral artery disease (PAD) affects more than 200 million people worldwide. PAD refers to illness due to a reduction or complete occlusion of blood flow in the artery, especially to the extremities in disease conditions, such as atherosclerosis or diabetes. Critical limb ischemia (CLI) is a severe form of PAD associated with high morbidity and mortality. Currently, no effective and permanent treatments are available for this disease. The current endovascular medications (e.g., angioplasty or stents) only relieve the clinical symptoms while the surgical therapies (e.g., bypass or endarterectomy) require grafting vessels from a healthy organ to the diseased limb of the patient. However, even with these therapeutic techniques, 30% of patients still undergo limb amputation within a year. Thus, understanding of disease mechanism and development of new therapeutic approaches are in urgent needs. Meis1 (myeloid ecotropic viral integration site 1) gene belongs to the three-amino-acid loop extension subclass of homeobox gene families, and it is a highly conserved transcription factor in all eukaryotes. Up to date, little is known about the role of Meis1 in regulating vascular remodeling under ischemic condition. In this study, we aim to investigate the role and underlying mechanism of Meis1 in the regulation of arteriogenesis and angiogenesis using hindlimb ischemia model of transgenic neonatal mice. The long-term goal is to develop a new treatment for patients with PAD. Three separate but related studies were planned to complete the proposed research aims. To better understand the role of Meis1, we reviewed, in the first chapter, all literature relevant to the recent advances of the Meis1 in normal hematopoiesis, vasculogenesis, and heart developments, which were mostly studied in zebrafish and mouse. Briefly, Meis1 is found to be highly expressed in the brain and retina in zebrafish and additional in the heart, nose, and limb in mouse during the very early developmental stage, and remains at a low level quickly after birth. Meis1 is necessary for both primitive and definitive hematopoiesis and required for posterior erythroid differentiation. The absence of Meis1 results in a severe reduction of the number of mature erythrocytes and weakens the heart beats in zebrafish. Meis1 deficiency mouse is dead as early as E11.5 due to the severe internal hemorrhage. In addition, Meis1 is essential in heart development. Knock-down of Meis1 can promote angiotensin II-induced cardiomyocytes (CMs) hypertrophy or CMs proliferation, which can be repressed by a transcription factor Tbx20. Meis1 appears to play a complicated role in the blood vessels. Although the major blood vessels are still normal when global deletion of Meis1, the intersegmental vessel cannot be formed in Meis1 morphants in the zebrafish, and the small vessels are either too narrow or form larger sinuses in Meis1 deficient mouse. The effects of Meis1 on the vascular network under normal and disease (ischemia) condition remain largely unknown, and the existing data in this field is limited. In the second chapter, we developed a method protocol to identify mice of all ages, especially neonates that we faced methodological difficulties to easily and permanently label prior to our major experiments. In this study, single- or 2-color tattooing (ear, tail, or toe or combinations) was performed to identify a defined or unlimited number of mice, respectively. Tail tattooing using both green and red pastes was suitable for identifying white-haired neonatal mice as early as postnatal day (PND) 1, whereas toe tattooing with green paste was an effective alternative approach for labeling black-haired mouse pups. In comparison, single-color (green) or 2-color (green and red) ear tattooing identified both white and black adult mice older than three weeks. Ear tattooing can be adapted to labeling an unlimited number of adult mice by adding the cage number. Thus, tattooing various combinations of the ears, tail, and toes provides an easy and permanent approach for identifying mice of all ages with minimal disturbance to the animals, which shows a new approach than any existing method to identify mouse at all ages, especially the neonatal pups used in the present study (Chapter 4). Various formation of hindlimb ischemia with ligations of femoral artery or vein or both have been reported in the literature. The ischemic severity varies dependent on mouse strains and ligation methods. Due to the tiny body size of our experimental neonatal mice (PND2), it is technically challenging to separate the femoral artery from femoral vein without potential bleeding. In the third chapter, we aimed to explore a suitable surgical approach that can apply to neonatal mice. To this end, we compared the effects of femoral artery/vein (FAV) excision vs. femoral artery (FA) excision on hindlimb model using adult CD-1 mice. We showed during the 4-week period of blood reperfusion, no statistically significant differences were found between FAV and FA excision-induced ischemia regarding the reduction of limb blood flow, paw size, number of necrotic toes, or skeletal muscle cell size. We conclude that FAV and FA excision in CD-1 mice generate a comparable severity of hindlimb ischemia. In other words, FAV ligation is no more severe than FA ligation. These findings provide valuable information for researchers when selecting ligation methods for their neonate hindlimb models. Based on these findings, we selected FAV ligation of hindlimb ischemia approach to study the function of Meis1 in vascular remodeling of neonatal mice. In the fourth chapter (the main part of my dissertation), we investigated the roles of Meis1 in regulating arteriogenesis and angiogenesis of neonatal mouse under the ischemic condition. To this end, endothelial cell-specific deletion of Meis1 was generated by cross-breeding Meis1flox/flox mice with Tie2-Cre mice. Wild-type (WT, Meis1f/f) and endothelial cell-specific knock-out (KO, Meis1ec-/-Tie2-Cre+) C57BL/6 mice at the age of PND2 were used. Under the anesthesia, the pups were subject to hindlimb ischemia by excising FAV. Laser Doppler Imager was used to measure the blood flow pre- and post-surgery up to 28 days. Toe necrosis, skeletal regeneration, and vascular distributions were examined at the end of experiments (PND28 post-ischemia). Surprisingly, during 4-week periods after ischemia, the blood flow ratios (ischemic vs. control limb) in KO mice significantly increased compared to WT on PND14 and PND28, suggesting the inhibitory effects of Meis1 on blood flow recovery under ischemic condition. Meanwhile, WT mice showed more severe necrotic limb (lower ratio of limb length and area, and higher necrotic scores at PND7) than those in the KO mice. Furthermore, significant increases in diameters of Dil-stained arterioles of the skin vessel and the vessels on the ligation site were observed in KO mice, indicating the enhanced arteriogenesis in KO mice. To investigate the underlying mechanism, RNA from the ischemia and control limb was extracted and q-PCR was used to study the potential genes involved in the mechanism. Casp3 and Casp8 were found downregulated showing less apoptosis in the KO mice. On the other hand, endothelial cells (ECs) were isolated from the lungs of 3-5 WT and KO neonates using CD31 Microbeads. CD31+ cells were plated and treated with 0, 0.5, and 1μM doxorubicin for 24 hours and analyzed with various assays. Meis1-KO ECs demonstrated higher cell viability and formed a higher number of vascular tubes than those in WT ECs following 0.5μM Dox treatment, presenting the potential ability of angiogenesis in KO-ECs. Furthermore, the increased viability in KO ECs may be due to the decreased expression or activities of Casp8 and Casp3. In conclusion, my present studies have developed a new methodology to easily and permanently identify all mice at any ages. The insignificant differences between FAV and FA ligations suggest that a relative-easy surgical approach could be used to generate hindlimb ischemic model, which potentially reduces the cost, decreases the surgical time and prevents damage of femoral nerve from surgical tools. More importantly, by using transgenic mice, we found that Meis1-KO dramatically increased blood flow and protected the ischemic hindlimb through vascular remodeling. Obviously, the molecular and cellular mechanisms underlying the above beneficial effects appear complicated and likely to involve multiple cellular remodeling processes and molecular signaling pathways to enhance arteriogenesis and angiogenesis and/or reduce cellular apoptosis through Meis1-mediated pathways. Our study demonstrated that under ischemic condition, knockout of Meis1 increases expression of Hif1a, which then activates Agt or VEGF, thus enhances arteriogenesis or angiogenesis; In addition, knockout of Meis1 activates Ccnd1, which subsequently promotes regeneration of skeletal muscle, and reduces expression of Casp8 and Casp3, thus preventing limb tissue from ischemia-induced apoptosis. Our innovative findings offer great potential to ultimately lead to new drug discovery or therapeutic approaches for prevention or treatment of PAD.
PHD

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38

Gaillard-Bigot, Florence. "Approches physiopathologiques et pharmacologiques de la fonction microvasculaire dans la Sclérodermie systémique." Thesis, Université Grenoble Alpes (ComUE), 2017. http://www.theses.fr/2017GREAS032/document.

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La microcirculation cutanée a été proposée comme modèle d’étude de la dysfonction microvasculaire globale dans les maladies cardiovasculaires. Par ailleurs, elle est spécifiquement atteinte dans la sclérodermie systémique (SSc), qui est une maladie dysimmunitaire rare, particulièrement invalidante, caractérisée par une fibrose cutanée et viscérale associée à une atteinte microvasculaire diffuse et la présence d’auto anticorps dirigés contre des antigènes cellulaires. L’exploration de la fonction microvasculaire cutanée suscite donc un réel intérêt, même s’il n’existe pas de technique standardisée pour l’étude de la fonction microvasculaire, en particulier endothéliale.La première partie de ce travail a porté sur l’étude physiologique de la microcirculation cutanée chez le volontaire sain, en utilisant les méthodes les plus récentes adaptées à l’étude fonctionnelle de la microcirculation (tests de réactivité vasculaire couplés à l’enregistrement du flux sanguin cutané par laser speckle contrast imaging). Dans une seconde partie, nous avons étudié la pathologie de la microcirculation cutanée dans la sclérodermie systémique, en utilisant les mêmes d’étude fonctionnelle de la microcirculation. La dernière partie de cette thèse a été consacrée à l’étude d’une nouvelle approche pharmacologique et thérapeutique dans la prise en charge des manifestations vasculaires cutanées périphériques identifiées chez les patients. Nous avons évalué l’effet vasodilatateur du tréprostinil, analogue de la prostacycline, sur le flux sanguin cutané de divers zones anatomiques, chez le volontaire sain, le patient atteint de SSc, le patient diabétique et lors d’un refroidissement local dans la SSc
Cutaneous microcirculation has been proposed as a model to study the global microvascular dysfunction occurring in cardiovascular diseases. Furthermore, it is specifically impaired in systemic sclerosis (SSc), which is a rare and particularly invalidating auto-immune disease, characterized by a cutaneous and visceral fibrosis, associated with a diffuse microvascular impairment and auto-antibodies targeting some cellular antigens. The study of cutaneous microvascular function provides a real interest despite the lack of available standardized techniques, particularly to explore endothelial microvascular function.In the first part of this work, we aimed to study the physiology of cutaneous microcirculation in healthy volunteers, using the more recent methods in this field, adapted to functional study of microcirculation (vascular reactivity tests coupled with cutaneous blood flow recording by laser speckle contrast imaging). The second part of our work aimed to study the pathology of cutaneous microcirculation in SSc volunteers, by using the same functional exploration methods. The last part of this work has been dedicated to a new pharmacologic and therapeutic approach for the management of peripheral cutaneous vascular manifestations in patients, using innovating technics as cutaneous iontophoresis. We studied the vasodilator effect of treprostinil, a prostacycline analogue, on cutaneous blood flow in several anatomic regions in healthy subject, SSc patient and diabetic patient, and also during a local cooling in SSc

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39

Phillipson, Mia. "Acid transport through gastric mucus : A study in vivo in rats and mice." Doctoral thesis, Uppsala University, Department of Medical Cell Biology, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3368.

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The gastric mucosa is frequently exposed to endogenously secreted hydrochloric acid of high acidity. Gastric mucosal defense mechanisms are arranged at different levels of the gastric mucosa and must work in unison to maintain its integrity.

In this thesis, several mechanisms underlying gastric mucosal resistance to strong acid were investigated in anesthetized rats and mice. The main findings were as follows:

Only when acid secretion occurred did the pH gradient in the mucus gel withstand back-diffusion of luminal acid (100 mM or 155 mM HCl), and keep the juxtamucosal pH (pH_jm) neutral. Thus, when no acid secretion occurred and the luminal pH was 0.8-1, the pH gradient was destroyed.

Bicarbonate ions, produced concomitant with hydrogen ions in the parietal cells during acid secretion and blood-borne to the surface epithelium, were carried transepithelially through a DIDS-sensitive transport.

Prostaglandin-dependent bicarbonate secretion seemed to be less important in maintaining a neutral pH_jm.

Removal of the loosely adherent mucus layer did not influence the maintenance of the pH_jm. Hence, only the firmly adherent mucus gel layer, approximately 80µm thick, seemed to be important for the pH_jm.

Staining of the mucus gel with a pH-sensitive dye revealed that secreted acid penetrated the mucus gel from the crypt openings toward the gastric lumen only in restricted paths (channels). One crypt opening was attached to one channel, and the channel was irreversibly formed during acid secretion.

Gastric mucosal blood flow increased on application of strong luminal acid (155 mM HCl). This acid-induced hyperemia involved the inducible but not the neural isoform of nitric oxide synthase. These results suggest a novel role for iNOS in gastric mucosal protection and indicate that iNOS is constitutively expressed in the gastric mucosa.

It is concluded that a pH gradient in the gastric mucus gel can be maintained during ongoing acid secretion, since the acid penetrates the mucus only in restricted channels and bicarbonate is carried from the blood to the lumen via a DIDS-sensitive transporter.

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40

Bergstrand, Sara. "Preventing pressure ulcers by assessment of the microcirculation in tissue exposed to pressure." Doctoral thesis, Linköpings universitet, Avdelningen för omvårdnad, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-109960.

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The overall aim of this thesis was to combine optical methods into a system with the ability to simultaneously measure blood flow changes at different tissue depths. The goal of such a system was to reveal vascular mechanisms relevant to pressure ulcer etiology under clinically relevant conditions and in relation to the evaluation of pressure-redistribution support surfaces. This thesis consists of four quantitative, cross-sectional studies measuring blood flow responses before, during, and after pressure exposure of the sacral tissue. Two optical methods – photoplethysmography and laser Doppler flowmetry – were combined in a newly developed system that has the ability to discriminate blood flows at different tissue depths. Studies I and II explored blood flow responses at different depths in 17 individuals. In Study I the blood flow was related to tissue thickness and tissue compression during pressure exposure of ≥ 220 mmHg. In Study II, the sacral tissue was loaded with 37.5 mmHg and 50.0 mmHg, and the variation in blood flow was measured. Studies III and IV included 42 healthy individuals < 65 years, 38 healthy individuals ≥ 65 years, and 35 patients ≥ 65 years. Study III included between-subject comparisons of blood flow and pressure between individuals in the three study groups lying in supine positions on a standard hospital mattress. Study IV added within-subject comparisons while the individual was lying on four different types of mattress. The studies explored the vascular phenomena pressure-induced vasodilation (PIV) and reactive hyperemia (RH). The most common blood flow response to tissue exposure in this thesis was PIV, although a decrease in blood flow (a lack of PIV) was observed in some individuals. The patients tended to have higher interface pressure during pressure exposure than the healthy groups but no differences in blood flow responses were seen. Our results showed that pressure levels that are normally considered to be harmless could have a significant effect on the microcirculation in different tissue structures. Differences in individual blood flow responses in terms of PIV and RH were seen, and a larger proportion of individuals lacked these responses in the deeper tissue structures compared to more superficial tissue structures. This thesis identified PIV and RH that are important vascular mechanisms for pressure ulcer development and revealed for the first time that PIV and RH are present at different depths under clinically relevant conditions. The thesis also identified a population of individuals not previously identified who lack both PIV and RH and seem to be particularly vulnerable to pressure exposure. Further, this thesis has added a new perspective to the microcirculation in pressure ulcer etiology in terms of blood flow regulation and endothelial function that are anchored in clinically relevant studies. Finally, the evaluation of pressureredistribution support surfaces in terms of mean blood flow during and after tissue exposure was shown to be unfeasible, but the assessment of PIV and RH could provide a new possibility for measuring individual physiological responses that are known to be related to pressure ulcer development.

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41

Lenge, Matteo. "Development and validation of innovative ultrasound flow imaging methods." Thesis, Lyon 1, 2015. http://www.theses.fr/2015LYO10036/document.

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L'échographie est largement utilisée pour l'imagerie du flux sanguin pour ses nombreux avantages tels que son inocuité, son cout réduit, sa facilité d'utilisation et ses performances. Cette thèse a pour objectif de proposer de nouvelles méthodes ultrasonores d'imagerie du flux sanguin. Après une étude bibliographique, plusieurs approches ont été étudiées en détail jusqu'à leur implémentation sur l'échographe de recherche ULA-OP développé au sein du laboratoire et ont été validées en laboratoire et en clinique. La transmission d'ondes planes a été proposée pour améliorer la technique d'imagerie utilisant les oscillations transverses. Des champs de pression ultrasonores présentant des oscillations transverses sont générés dans de larges régions et exploités pour l'estimation vectorielle du flux sanguin à une haute cadence d'imagerie. Des cartes du flux sanguin sont obtenues grâce à une technique s'appuyant sur la transmission d'ondes planes couplées à un nouvel algorithme d'estimation de la vitesse dans le domaine fréquentiel. Les méthodes vectorielles implémentées en temps réel dans le ULA-OP ont été comparées à la méthode Doppler classique lors d'une étude clinique. Les résultats ont montré le bénéfice des méthodes vectorielles en termes de précision et de répétabilité. La nouvelle méthode proposée a démontré sa grande précision ainsi que son gain en termes de temps de calcul aussi bien en simulations qu'en acquisitions en laboratoire ou lors d'essais in vivo. Une solution logicielle temps réel implémentée sur une carte GPU a été proposée et testée afin de réduire encore le temps de calcul et permettre l'emploi de la méthode en clinique
Ultrasound is widely used for blood flow imaging because of the considerable advantages for the clinician, in terms of performance, costs, portability, and ease of use, and for the patient, in terms of safety and rapid checkup. The undesired limitations of conventional methods (1-D estimations and low frame-rate) are widely overtaken by new vector approaches that offer detailed descriptions of the flow for a more accurate diagnosis of cardiovascular system diseases. This PhD project concerns the development of novel methods for blood flow imaging. After studying the state-of-the-art in the field, a few approaches have been examined in depth up to their experimental validation, both in technical and clinical environments, on a powerful ultrasound research platform (ULA-OP). Real-time novel vector methods implemented on ULA-OP were compared to standard Doppler methods in a clinical study. The results attest the benefits of the vector methods in terms of accuracy and repeatability. Plane-wave transmissions were exploited to improve the transverse oscillation imaging method. Double oscillating fields were produced in large regions and exploited for the vectorial description of blood flow at high frame rates. Blood flow maps were obtained by plane waves coupled to a novel velocity estimation algorithm operating in the frequency domain. The new method was demonstrated capable of high accuracy and reduced computational load by simulations and experiments (also in vivo). The investigation of blood flow inside the common carotid artery has revealed the hemodynamic details with unprecedented quality. A software solution implemented on a graphic processing unit (GPU) board was suggested and tested to reduce the computational time and support the clinical employment of the method

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42

Petersson, Joel. "Nitrate, Nitrite and Nitric Oxide in Gastric Mucosal Defense." Doctoral thesis, Uppsala University, Department of Medical Cell Biology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8624.

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The human stomach normally contains high levels of bioactive nitric oxide (NO). This NO derives from salivary nitrate (NO₃^-) that is converted to nitrite (NO₂^-) by oral bacteria and thereafter non-enzymatically reduced in the acidic gastric lumen to NO. Nitrate is a common component in vegetables, and after ingestion it is absorbed in the small intestine. Interestingly, circulating nitrate is then concentrated by the salivary glands. Hence, intake of nitrate-rich vegetables results in high levels of NO in the stomach. The physiological effects of the high concentration of NO gas normally present in the gastric lumen have been hitherto unknown, and the present investigations were therefore conducted to address this issue.

NO produced in the gastric lumen after nitrate ingestion increased gastric mucosal blood flow and the thickness of the firmly adherent mucus layer in the stomach. The blood flow and mucus layer are essential defense mechanisms that protect the mucosa from luminal acid and noxious agents. Nonsteroidal antiinflammatory drugs (NSAID) are commonly prescribed and effective drugs for treating pain and inflammation, but are associated with severe gastrointestinal side effects. We demonstrated that a nitrate-rich diet protects against NSAID-induced gastric damage, as a result of the increased formation of NO in the stomach. We also showed that the gastroprotective effect attributed to nitrate depended completely on conversion of nitrate to nitrite by the bacterial flora colonizing the tongue, and that the oral microflora is therefore important in regulating physiological conditions in the stomach.

In summary, this thesis challenges the current dogma that nitrate intake is hazardous, and on the contrary suggests that dietary nitrate plays a direct role in regulating gastric homeostasis. It is likely that a sufficient supply of nitrate in the diet together with the oral microflora is essential for preventing pathological conditions in the gastrointestinal tract.

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43

Jahůdková, Michaela. "Optické metody pro měření průtoku založené na kontrastu speklí." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2017. http://www.nusl.cz/ntk/nusl-316827.

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Анотація:

This thesis deals with introduction to optical method called laser speckle contrast imaging (LSCI). The thesis contains a description of the basic theory that is related to the method, the LSCI own principle, the characteristics of this technique, its applications and also a description of various variants of LSCI. The second part of the thesis deals with the modification of acquisition software to be suitable for measurement of multi-exposure LSCI (MESI), verification of this method by experiment and conclusions of evaluated of data.

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44

Ledford, Benjamin. "Keratose Hydrogels Promote Vascular Smooth Muscle Differentiation from c-kit+ Human Cardiac Stem Cells: Underlying Mechanism and Therapeutic Potential." Diss., Virginia Tech, 2018. http://hdl.handle.net/10919/93593.

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Анотація:

Cardiovascular disease is the leading cause of death in the United States, and coronary artery disease (CAD) kills over 370,000 people annually. There are available therapies that offer a temporary solution; however, only a heart transplant can fully resolve heart failure, and donor organ shortages severely limit this therapy. C-kit+ human cardiac stem cells (hCSCs) offers a viable alternative therapy to treat cardiovascular disease by replacing damaged cardiac tissue; however, low cell viability, low retention/engraftment, and uncontrollable in vivo differentiation after transplantation has limited the efficacy of stem cell therapy. Tissue engineering solutions offer potential tools to overcome current limitations of stem cell therapy. Materials derived from natural sources such as keratin from human hair offers innate cellular compatibility, bioactivity, and low immunogenicity. Keratin proteins extracted using oxidative chemistry known as keratose (KOS) have shown therapeutic potential in a wide range of applications including cardiac regeneration. My studies utilize KOS hydrogels to modulate c-kit+ hCSC differentiation, and explore the capability of differentiated cells to regenerate vascular tissue. In the first Chapter, we reviewed literature relevant to keratin-based biomaterials and their biomedical applications, the use of stem cells in cardiovascular research, and the differentiation of vascular smooth muscle cells (VSMCs). The section on biomedical applications of keratin biomaterials focuses on the oxidized form of keratin known as keratose (KOS), because this was the material used for our research. Since we planned to use this material in conjunction with c-kit+ hCSCs, we also briefly explored the use of stem cells in cardiovascular research. Additionally, we examined some key signaling pathways, developmental origins, and the cell phenotype of VSMCs for reasons that will become clear after observing results from chapters 2 and 3. Based upon our review of the literature, KOS biomaterials and c-kit+ hCSCs were determined to be promising as a combined therapeutic for the regeneration of cardiac tissue. Research in Chapter 2 focused on characterizing the effects of KOS hydrogel on c-kit+ hCSC cell viability, proliferation, morphology, and differentiation. Results demonstrated that KOS hydrogels could maintain hCSC viability without any observable toxic effects, but it modulated cell size, proliferation, and differentiation compared to standard tissue culture polystyrene cell culture (TCPS). KOS hydrogel produced gene and protein expression consistent with a VSMC phenotype. Further, we also observed novel "endothelial cell tube-like" microstructures formed by differentiated VSMCs only on KOS hydrogel, suggesting a potential capability of the hCSC-derived VSMCs for in vitro angiogenesis. Results from this study lead us to question what signaling pathways might be responsible for the apparent VSMC differentiation pattern we observed on KOS hydrogels. Research in Chapter 3 explored the time course of VSMC differentiation, cell contractility, inhibition of VSMC differentiation, and measured protein expression of transforming growth factor beta 1 (TGF-β1) and its associated peptides for hCSCs cultured on KOS hydrogels, tissue culture polystyrene, and collagen hydrogels. A review of VSMC differentiation signaling pathways informed our decision to investigate the role of TGF-β1 in VSMC differentiation. Results demonstrated that KOS hydrogel differentiated hCSCs significantly increased expression for all three vascular smooth muscle (VSM) markers compared to TCPS differentiated cells. Additionally, KOS differentiated hCSCs were significantly more contractile than cells differentiated on TCPS. Recombinant human (rh) TGF-β1 was able to induce VSM differentiation on TCPS. VSM differentiation was successfully inhibited using TGF-β NABs and A83-01. Enzyme-Linked Immunosorbent Assay (ELISA) analysis revealed that both TCPS and KOS hydrogel differentiated cells produced TGF-β1, with higher levels being measured at early time points on TCPS and later time points on KOS hydrogels. Results from supplementing rhTGF-β1 to TCPS and KOS hydrogels revealed that KOS seems to interact with TGF-β to a greater extent than TCPS. Western blot results revealed that latency TGFβ binding protein (LTBP-1) and latency associated peptide (LAP) had elevated levels early during differentiation. Further, the levels of LTBP-1 and LAP were higher on KOS differentiated hCSCs than TCPS hCSCs. This study reaffirms previous results of a VSM phenotype observed on KOS hydrogels, and provides convincing evidence for TGF-β1 inducing VSM differentiation on KOS hydrogels. Additionally, results from ELISA and western blot provide evidence that KOS plays a direct role in this pathway via interactions with TGF-β]1 and its associated proteins LTBP-1 and LAP. Results from chapter 2 and 3 offered significant evidence that our cells exhibited a VSMC phenotype, and that TGF-β1 signaling was a key contributor for the observed phenotype, but we still needed an animal model to explore the therapeutic potential of our putative VSMCs. Research in Chapter 4 investigated a disease model to test the ability of KOS hydrogel differentiated cells to regenerate vascular tissue. To measure vascular regenerative capability, we selected a murine model of critical limb ischemia (CLI). CLI was induced in 3 groups (n=15/group) of adult mixed gender NSG mice by excising the femoral artery and vein, and then treated the mice with either PBS (termed as PBS-treated), Cells differentiated on TCPS (termed as Cells from TCPS), or KOS hydrogel-derived VSMCs (termed as Cells from KOS). Blood perfusion of the hind limbs was measured immediately before and after surgery, then 14, and 28 days after surgery using Laser Doppler analysis. Tissue vascularization, cell engraftment, and skeletal muscle regeneration were measured using immunohistochemistry, 1,1'-Dioctadecyl3,3,3',3'-Tetramethylindocarbocyanine Perchlorate (DiL) vessel painting, and hematoxylin and eosin (HandE) pathohistological staining. During the 4-week period, both cell treatment groups showed significant increases in blood perfusion compared to the PBS-treated control, and at day 28 the Cells from KOS group had significantly better blood flow than the Cells from TCPS group. Additionally, the Cells from KOS group demonstrated a significant increase in the ratio of DiL positive vessels, capillary density, and a greater density of small diameter arterioles compared to the PBS-treated group. Further, both cell-treated groups had similar levels of engraftment into the host tissue. We conclude that Cells from KOS therapy increases blood perfusion in an NSG model of CLI, but does not lead to increased cell engraftment compared to other cell based therapies. Overall, the results from this dissertation demonstrated that KOS hydrogels produce VSMC differentiation from c-kit+ hCSCs mediated by TGF-β1 signaling, and that the differentiated cells are able to increase blood perfusion in a CLI model by increasing capillary density, suggesting enhanced angiogenesis. Future studies should explore potential protein-protein interactions between KOS, TGF-β1 and its associated proteins. Additionally, we should plan animal studies that examine the efficacy of our cells to regenerate cardiac tissue following an acute myocardial infarction (AMI).
PHD

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45

Hoštáková, Nina. "Detekce průtoku pomocí optických interferenčních metod." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2015. http://www.nusl.cz/ntk/nusl-221336.

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The thesis deals with LSCI (Laser Speckle Contrast Imaging), an optical method utilizing laser speckle contrast for the estimation of blood flow changes. LSCI is non-invasive and technically not demanding approach, capabilities of which have not yet been fully exploited. The literature review part contains detailed description of the operating principle, imaging techniques, potential for medical applications with considering the limiting factors. The main aim of the thesis is to design and construct a complete LSCI system including appropriate phantoms able to simulate blood flow through the tissue. Imaging algorithms for the obtained data evaluation were implemented in Matlab® development enviroment. Finally, the created system was tested using different acquisition parameters as well as varying the image processing schemes. The resulting qualitative flow images were subsequently discussed and confronted with the theoretical assumptions.

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46

Kroppenstedt, Stefan Nikolaus. "Die Bedeutung des zerebralen Perfusionsdruckes in der Behandlung des schweren Schädel-Hirn-Traumes." Doctoral thesis, Humboldt-Universität zu Berlin, Medizinische Fakultät - Universitätsklinikum Charité, 2003. http://dx.doi.org/10.18452/13898.

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Die Höhe des optimalen zerebralen Perfusionsdruckes nach schwerem Schädel-Hirn-Trauma wird kontrovers diskutiert. Während im sogenannten Lund-Konzept ein niedriger Perfusionsdruck angestrebt und die Gabe von Katecholaminen aufgrund potentieller zerebraler vasokonstringierender und weiterer Nebeneffekte vermieden wird, befürwortet das CPP-Konzept nach Rosner eine Anhebung des zerebralen Perfusionsdruckes, wenn notwendig unter intravenöser Gabe von Katecholaminen. Vor diesem Hintergrund galt es, in einem experimentellen Schädel-Hirn-Trauma- Modell der Ratte (Controlled Cortical Impact Injury) den Bereich des optimalen zerebralen Perfusionsdruckes nach traumatischer Hirnkontusion zu ermitteln und den Effekt von Katecholaminen auf den posttraumatischen zerebralen Blutfluss und die Entwicklung des sekundären Hirnschadens zu untersuchen. Die wesentlichen Ergebnisse dieser Arbeit lassen sich wie folgt zusammenfassen: In der Akutphase nach Hirnkontusion liegt der Bereich des zerebralen Perfusionsdruckes, welcher die Entwicklung des Kontusionsvolumens nicht beeinflusst, zwischen 70 und 105 mm Hg. Eine Senkung des Perfusionsdruckes unterhalb bzw. Anhebung oberhalb dieser Schwellenwerte vergrößert das Kontusionsvolumen. Die Anhebung des Blutdruckes mittels intravenöser Infusion von Dopamin oder Noradrenalin führt sowohl in der Frühphase als auch in der Spätphase nach Trauma (4 Stunden bzw. 24 Stunden nach kortikaler Kontusion) zu einem signifikanten Anstieg im kortikalen perikontusionellen Blutfluss und in der Hirngewebe-Oxygenierung. Die durch Anhebung des zerebralen Perfusionsdruckes auf über 70 mm Hg induzierte Verbesserung des posttraumatischen zerebralen Blutflusses bewirkte jedoch keine Reduzierung der Hirnschwellung. Für eine Katecholamin-induzierte zerebrale Vasokonstriktion nach kortikaler Kontusion gibt es keinen Anhalt. Um die Entwicklung des sekundären Hirnschadens nach kortikaler Kontusion zu minimieren, sollte der zerebrale Perfusionsdruck nach traumatischem Hirnschaden nicht unterhalb 70 mm Hg liegen. Eine Anhebung des Perfusionsdruckes auf über 70 mm Hg erscheint nicht notwendig oder vorteilhaft zu sein. Wenn notwendig, kann sowohl in der Früh- als auch Spätphase nach Trauma der zerebrale Perfusionsdruck mittels intravenöser Gabe von Katecholaminen angehoben werden.
The optimum cerebral perfusion pressure after severe traumatic brain injury remains to be controversial. In the Lund concept a relatively low cerebral perfusion pressure is preferred, and administration of catecholamines is avoided due to potential catecholamine-mediated cerebral vasoconstriction and other side effects. In contrast, the CPP concept of Rosner recommends elevation of cerebral perfusion pressure, if needed by intravenous administration of catecholamines. Based on this, in an experimental model of traumatic brain injury of the rat (Controlled Cortical Impact Injury) the optimum range of cerebral perfusion pressure after traumatic brain contusion and the effects of catecholamines on posttraumatic cerebral perfusion and development of secondary brain injury were investigated. The most significant results can be summarized as follows: In the acute phase after brain contusion the range of cerebral perfusion pressure that does not affect the development of posttraumatic contusion volume was found to be between 70 and 105 mm Hg. Reduction of the cerebral perfusion pressure below or elevation above these thresholds increases contusion volume. Elevation of blood pressure by intravenous infusion of dopamine or norepinephrine during the early (4 hours) as well as late (24 hours) phase after trauma results in a significant increase in pericontusional blood flow and brain tissue oxygenation. The increase in cerebral blood flow by elevating cerebral perfusion pressure above 70 mm Hg did not decrease cerebral edema formation. There was no evidence of a catecholamine-induced cerebral vasoconstriction after cortical contusion. In order to minimize secondary brain injury after cortical contusion, cerebral perfusion pressure should not fall bellow 70 mm Hg. However, a further active elevation of cerebral perfusion pressure does not appear necessary or beneficial. If needed cerebral perfusion pressure can be elevated by administration of catecholamines in the early as well late phase after trauma.

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47

Soubeyrand, Marc. "Etude de la perfusion médullaire après lésion traumatique de la moelle épinière à dure-mère intacte." Thesis, Paris 11, 2012. http://www.theses.fr/2012PA114837.

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Après un traumatisme de la moelle épinière (TM), l’ischémieest un facteur d’aggravation des lésions. Cette ischémie peut être aggravée par l’augmentation depression du liquide cérébro-spinal (LCS) par le biais d’un effet tamponnade. Or chez l’homme,après un TM avec préservation de l’intégrité de la dure-mère, la pression de LCS augmentesignificativement. On suppose donc que le maintien d’une pression de LCS à des valeursphysiologique pourrait être une méthode de limitation de l’ischémie post-traumatique et doncd’amélioration du pronostic fonctionnel. Afin de pouvoir réaliser une étude expérimentale de cesphénomènes, nous avons consacré la première partie expérimentale de cette thèse à la mise au pointd’un modèle de TM à dure-mère intacte chez le rat permettant la mesure simultanée de la pressionde LCS et de la perfusion médullaire. Nous avons confirmé expérimentalement que la pression deLCS augmente après TM. Dans la seconde partie expérimentale, nous avons mis au point unetechnique expérimentale de quantification spatiale et temporelle de la perfusion médullaire grâce àl’échographie de contraste. Cette technique permettait aussi un suivi en temps réel de l’évolution dusaignement intra-parenchymateux induit par le TM. Dans la troisième partie expérimentale, nousavons utilisé notre modèle couplé avec l’échographie de contraste et le laser Doppler pour évaluerles effets de la noradrénaline injectée à la phase aigüe d’un TM sur la perfusion médullaire et lesaignement intra-parenchymateux. Nous avons montré que la noradrénaline augmentait trèslégèrement le flux sanguin superficiel mais pas le flux sanguin profond et qu’elle augmentait lataille du saignement
After spinal cord injury (SCI), ischaemia aggravates lesions.Increase in cerebrospinal fluid (CSF) pressure can worsens ischaemia through a tamponnade effect.In humans, it has been shown that after SCI with intact dura mater, CSF pressure significantlyincreases. Therefore, preserving CSF pressure within a physiological range may limit post-traumaischaemia and improve neurological outcome. In order to experimentally study these phenomenon,we have dedicated the first part of that work to create a model of SCI in rats preserving dura’sintegrity and allowing simultaneous measurement of spinal cord blood flow (SCBF) and CSFpressure. We have confirmed that CSF pressure increases after SCI with intact dura. In the secondexperimental part, we have developed a technique allowing to perform spatial and temporalmeasurement of SCBF thanks to contrast enhanced ultrasonography (CEU). Moreover, thistechnique allows real-time measurement of the size of the parenchymal hemorrhage. In the thirdexperimental part, we have used our experimental model in association with CEU and LaserDoppler to assess the effects of early injection of norepinephrine on SCBF and parenchymalhemorrhage. We found that norepinephrine induces a slight increase in superficial SCBF while itdoesn’t modify deep SCBF and significantly increases the size of parenchymal hemorrhage

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48

Henriksnäs, Johanna. "Helicobacter pylori and Gastric Protection Mechanisms : An in vivo Study in Mice and Rats." Doctoral thesis, Uppsala universitet, Institutionen för medicinsk cellbiologi, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5898.

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The stomach is frequently exposed to hazardous agents and to resist this harsh environment, several protective mechanisms exist. Of special interest is the gastric pathogen Helicobacter pylori which causes gastritis, ulcers and cancer but the mechanism leading to these diseases are still unclear. However it is very likely that H. pylori negatively influence the protection mechanisms that exist in the stomach. The aims of the present investigation were first to develop an in vivo mouse model in which different protection mechanisms could be studied, and second to investigate the influence of H. pylori on these mechanisms. An in vivo preparation of the gastric mucosa in mice was developed. This preparation allows studies of different gastric mucosal variables and can also be applied for studies in other gastro-intestinal organs. Mice chronically infected with H. pylori, were shown to have a reduced ability of the mucosa to maintain a neutral pH at the epithelial cell surface. This could be due to the thinner inner, firmly adherent mucus gel layer, and/or to defective bicarbonate transport across the epithelium. The Cl-/HCO3- exchanger SLC26A9 was inhibited by NH4+, which also is produced by H. pylori. The mRNA levels of SLC26A9 were upregulated in infected mice, suggesting a way to overcome the inhibition of the transporter. Furthermore, the hyperemic response to acid pH 2 and 1.5 was abolished in these mice. The mechanisms by which the bacteria could alter the blood flow response might involve inhibition of the epithelial iNOS. Water extracts of H. pylori (HPE) reduces the blood flow acutely through an iNOS and nerve-mediated pathway, possibly through the endogenous iNOS inhibitor ADMA. Furthermore, HPE alters the blood flow response to acid as the hyperemic response to acid pH 0.8 is accentuated in mice treated with HPE.

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49

Jouette, Christian. "Conception d'un appareil de mesure du débit sanguin tissulaire par effet doppler optique : choix des paramètres d'échantillonnage et d'analyse spectrale, et détermination d'un indice débit métrique." Nancy 1, 1994. http://www.theses.fr/1994NAN10106.

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La mesure du débit sanguin tissulaire par effet doppler optique est une idée séduisante. L'utilisation d'une source laser, par la cohérence spatiale et géométrique qu'elle offre, en simplifie la réalisation pratique. La vitesse des différentes composantes, notamment les hématies, n'est pas uniforme. Il en résulte donc une distribution spectrale image de cette répartition. De plus, l'environnement du faisceau laser étant constitué par de la matière vivante, de nombreux paramètres influencent les résultats. Dans un premier temps, nous décrivons l'évolution des doppler-lasers, et nous exposons les problèmes qu'ils soulèvent. Ceci nous amène à considérer ensuite, que seule une étude fine du spectre peut nous conduire à un appareil fiable et réellement utilisable en routine. Nous présentons ici un doppler-laser à traitement numérique. La distribution spectrale est calculée par un processeur de signaux numérique (dsp) grâce à un algorithme de transformée de Fourier rapide. Nous donnons les paramètres caractéristiques qui nous paraissent optimaux, et relevons les difficultés qui subsistent avant de pouvoir réaliser un système performant

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50

Smirni, Salvatore. "Nonlinear dynamics of microcirculation and energy metabolism for the prediction of cardiovascular risk." Thesis, University of Dundee, 2018. https://discovery.dundee.ac.uk/en/studentTheses/c551cbef-6f00-48ef-b753-ad76ac93daf4.

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The peripheral skin microcirculation reflects the overall health status of the cardiovascular system and can be examined non-invasively by laser methods to assess early cardiovascular disease (CVD) risk factors, i.e. oxidative stress and endothelial dysfunction. Examples of methods used for this task are the laser Doppler flowmetry (LDF) and laser fluorescence spectroscopy (LFS), which respectively allow tracing blood flow and the amounts of the coenzyme NAD(P)H (nicotamide adenine dinucleotide) that is involved in the cellular production of ATP (adenosine triphosphate) energy. In this work, these methods were combined with iontophoresis and PORH (post-occlusive reactive hyperaemia) reactive tests to assess skin microvascular function and oxidative stress in mice and human subjects. The main focus of the research was exploring the nonlinear dynamics of skin LDF and NAD(P)H time series by processing the signals with the wavelet transform analysis. The study of nonlinear fluctuations of the microcirculation and cell energy metabolism allows detecting dynamic oscillators reflecting the activity of microvascular factors (i.e. endothelial cells, smooth muscle cells, sympathetic nerves) and specific patterns of mitochondrial or glycolytic ATP production. Monitoring these dynamic factors is powerful for the prediction of general vascular/metabolic health conditions, and can help the study of the mechanisms at the basis of the rhythmic fluctuations of micro-vessels diameter (vasomotion). In this thesis, the microvascular and metabolic dynamic biomarkers were characterised in-vivo in a mouse model affected by oxidative stress and a human cohort of smokers. Data comparison, respectively, with results from control mice and non-smokers, revealed significant differences suggesting the eligibility of these markers as predictors of risk associated with oxidative stress and smoke. Moreover, a relevant link between microvascular and metabolic oscillators was observed during vasomotion induced by α-adrenergic (in mice) or PORH (in humans) stimulations, suggesting a possible role of cellular Ca²⁺oscillations of metabolic origin as drivers of vasomotion which is a theory poorly explored in literature. As future perspective, further exploration of these promising nonlinear biomarkers is required in the presence of risk factors different from smoke or oxidative stress and during vasomotion induced by stimuli different from PORH or α-adrenergic reactive challenges, to obtain a full picture on the use of these factors as predictors of risk and their role in the regulation of vasomotion.

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