Увійти

Готові списки джерел за темами / Laser-assisted microdissection / Статті в журналах

Статті в журналах з теми "Laser-assisted microdissection"

Щоб переглянути інші типи публікацій з цієї теми, перейдіть за посиланням: Laser-assisted microdissection.

Автор: Grafiati

Опубліковано: 7 липня 2024

Оновлено: 7 липня 2024

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями

Оберіть тип джерела:

Ознайомтеся з топ-50 статей у журналах для дослідження на тему "Laser-assisted microdissection".

Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.

Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.

Переглядайте статті в журналах для різних дисциплін та оформлюйте правильно вашу бібліографію.

1

Chimge, Nyam-Osor, Frank Ruddle, and Dashzeveg Bayarsaihan. "Laser-assisted microdissection (LAM) in developmental biology." Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 308B, no. 2 (March 15, 2007): 113–18. http://dx.doi.org/10.1002/jez.b.21133.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

2

Sirivatanauksorn, Yongyut, Rosybel Drury, Tatjana Crnogorac-Jur?evi?, Vorapan Sirivatanauksorn, and Nicholas R. Lemoine. "Laser-assisted microdissection: applications in molecular pathology." Journal of Pathology 189, no. 2 (October 1999): 150–54. http://dx.doi.org/10.1002/(sici)1096-9896(199910)189:2<150::aid-path451>3.0.co;2-g.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

3

Day, Robert C., Ueli Grossniklaus, and Richard C. Macknight. "Be more specific! Laser-assisted microdissection of plant cells." Trends in Plant Science 10, no. 8 (August 2005): 397–406. http://dx.doi.org/10.1016/j.tplants.2005.06.006.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

4

Blakey, Gregory L., and Zoltan G. Laszik. "Laser-assisted microdissection of the kidney: Fundamentals and applications." Histochemical Journal 35, no. 6 (August 2004): 581–87. http://dx.doi.org/10.1007/s10735-004-2195-5.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

5

Pinzani, P., C. Orlando, and M. Pazzagli. "Laser-assisted microdissection for real-time PCR sample preparation." Molecular Aspects of Medicine 27, no. 2-3 (April 2006): 140–59. http://dx.doi.org/10.1016/j.mam.2005.12.006.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

6

Small, HJ, J. Sturve, JP Bignell, M. Longshaw, BP Lyons, R. Hicks, SW Feist, and GD Stentiford. "Laser-assisted microdissection: a new tool for aquatic molecular parasitology." Diseases of Aquatic Organisms 82 (November 20, 2008): 151–56. http://dx.doi.org/10.3354/dao01983.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

7

Fink, Ludger, Stephanie Kohlhoff, Maria Magdalena Stein, Jörg Hänze, Norbert Weissmann, Frank Rose, Ercan Akkayagil, et al. "cDNA Array Hybridization after Laser-Assisted Microdissection from Nonneoplastic Tissue." American Journal of Pathology 160, no. 1 (January 2002): 81–90. http://dx.doi.org/10.1016/s0002-9440(10)64352-0.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

8

Prasad, Rachana, and Ajay Mallick. "Comparison of Microdissection Microlaryngeal Surgery with Carbon Dioxide Laser in Management of Benign and Premalignant Lesions of Larynx." Bengal Journal of Otolaryngology and Head Neck Surgery 26, no. 3 (December 7, 2018): 190–96. http://dx.doi.org/10.47210/bjohns.2018.v26i3.208.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Introduction Dysphonia is caused by voice misuse and various environmental factors. It is manifested as varied pathological lesions of the vocal cords. Surgical excision of these lesions is mainly by conventional cold steel or laser assisted microsurgical techniques. Both modalities have seen extensive advancements and refinement in technologies in the recent past. In this study we have compared the microdissection microlaryngeal treatment and microspot superpulsed beam carbon dioxide (CO2) laser assisted surgical techniques for the management of benign and precancerous lesions of the vocal cords. Material and Methods A total of 36 cases of benign lesions of vocal cord, were divided randomly to undergo surgery either by microdissection or CO2laser assisted techniques. The groups were assessed through vocal cord morphological observation and subjective voice assessment parameters GRBAS score and VHI10 index. Results Peroperative bleeding was observed to be significantly reduced in the laser excision group. However, operating time was significantly increased in this group. Patients recovered remarkably well following both the techniques as denoted by voice parameters. No difference was observed in duration of hospital stay. Conclusion Both surgical techniques give satisfactory results in their management of benign and precancerous lesions of the vocal cords. Both techniques have their advantages and disadvantages. After a learning curve, laser surgery with its precision and cleaner surgical fields will be more effective in the management of such cases.

9

Montag Ph.D., Markus, Katrin van der Ven M.D., Guy Delacrétaz Ph.D., Klaus Rink Ph.D., and Hans van der Ven M.D. "Laser-Assisted Microdissection of the Zona Pellucida Facilitates Polar Body Biopsy." Fertility and Sterility 69, no. 3 (March 1998): 539–42. http://dx.doi.org/10.1016/s0015-0282(97)00538-4.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

10

Kuhn, Donald E., Sashwati Roy, Jared Radtke, Sudip Gupta, and Chandan K. Sen. "Laser microdissection and pressure-catapulting technique to study gene expression in the reoxygenated myocardium." American Journal of Physiology-Heart and Circulatory Physiology 290, no. 6 (June 2006): H2625—H2632. http://dx.doi.org/10.1152/ajpheart.01346.2005.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

For focal events such as myocardial infarction, it is important to dissect infarction-induced biological responses as a function of space with respect to the infarct core. Laser microdissection pressure catapulting (LMPC) represents a recent variant of laser capture microdissection that enables robot-assisted rapid capture of catapulted tissue without direct user contact. This work represents the maiden effort to apply laser capture microdissection to study spatially resolved biological responses in myocardial infarction. Infarcted areas of the surviving ischemic-reperfused murine heart were identified using a standardized hematoxylin QS staining procedure. Standard staining techniques fail to preserve tissue RNA. Exposure of the tissue to an aqueous medium (typically used during standard immunohistochemical staining), with or without RNase inhibitors, resulted in a rapid degradation of genes, with ∼80% loss in the 1st h. Tissue elements (1 × 104–4 × 106 μm2) captured from infarcted and noninfarcted sites with micrometer-level surgical precision were collected in a chaotropic RNA lysis solution. Isolated RNA was analyzed for quality by microfluidics technology and reverse transcribed to generate high-quality cDNA. Real-time PCR analysis of the cDNA showed marked (200- and 400-fold, respectively) induction of collagen Ia and IIIa at the infarcted site compared with the noninfarcted site. This work reports a sophisticated yet rapid approach to measurement of relative gene expressions from tissue elements captured from spatially resolved microscopic regions in the heart with micrometer-level precision.

11

Mitchell, Natalie M., Surendra Dasari, Thomas E. Grys, and Douglas F. Lake. "Laser Capture Microdissection-Assisted Protein Biomarker Discovery from Coccidioides-Infected Lung Tissue." Journal of Fungi 6, no. 4 (December 14, 2020): 365. http://dx.doi.org/10.3390/jof6040365.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Laser capture microdissection (LCM) coupled to label-free quantitative mass spectrometry is a viable strategy to identify biomarkers from infected tissues. In this study, LCM was employed to take a “snapshot” of proteins produced in vivo during Coccidiodies spp. infection in human lungs. Proteomic analysis of LCM lung sections revealed hundreds of hosts and Coccidioidal proteins. Twenty-seven highly abundant Coccidioides spp. proteins were identified which do not share significant sequence orthology with human proteins. Three of the 27 Coccidioidal proteins are also potential Coccidoides-specific biomarkers, as they also do not share sequence homology to any other pathogenic fungus or microbe. Gene ontology analysis of the 27 biomarker candidate proteins revealed enriched hydrolase activity and increased purine and carbohydrate metabolism functions. Finally, we provide proteomic evidence that all 27 biomarker candidates are produced by the fungus when grown in vitro in a media- and growth-phase dependent manner.

12

Yao, Virginia J., Michael G. Ozawa, Martin Trepel, Wadih Arap, Donald M. McDonald, and Renata Pasqualini. "Targeting Pancreatic Islets with Phage Display Assisted by Laser Pressure Catapult Microdissection." American Journal of Pathology 166, no. 2 (February 2005): 625–36. http://dx.doi.org/10.1016/s0002-9440(10)62283-3.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

13

Eberle, Franziska C., Jeffrey C. Hanson, J. Keith Killian, Lai Wei, Kris Ylaya, Stephen M. Hewitt, Elaine S. Jaffe, Michael R. Emmert-Buck, and Jaime Rodriguez-Canales. "Immunoguided Laser Assisted Microdissection Techniques for DNA Methylation Analysis of Archival Tissue Specimens." Journal of Molecular Diagnostics 12, no. 4 (July 2010): 394–401. http://dx.doi.org/10.2353/jmoldx.2010.090200.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

14

von der Hardt, Katharina, Michael Andreas Kandler, Ludger Fink, Ellen Schoof, Jörg Dötsch, Rainer Maria Bohle, and Wolfgang Rascher. "Laser-assisted microdissection and real-time PCR detect anti-inflammatory effect of perfluorocarbon." American Journal of Physiology-Lung Cellular and Molecular Physiology 285, no. 1 (July 2003): L55—L62. http://dx.doi.org/10.1152/ajplung.00198.2002.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

The aim of this study was to identify cell types involved in the anti-inflammatory effect of ventilation with perfluorocarbon in vivo. Fifteen anesthetized, surfactant-depleted piglets received either aerosolized perfluorocarbon (Aerosol-PFC), partial liquid ventilation (rLV) at functional residual capacity (FRC) volume (FRC-PLV), or intermittent mandatory ventilation (control). After laser-assisted microdissection of different lung cell types, mRNA expression of IL-8 and ICAM-1 was determined using TaqMan real-time PCR normalized to hypoxanthine phosphoribosyltransferase (HPRT). IL-8 mRNA expression (means ± SE; control vs. Aerosol-PFC) was 356 ± 142 copies IL-8 mRNA/copy HPRT mRNA vs. 3.5 ± 1.8 in alveolar macrophages ( P <0.01); 208 ± 108 vs. 2.7 ± 0.8 in bronchiolar epithelial cells ( P <0.05); 26 ± 11 vs. 0.7 ± 0.2 in alveolar septum cells ( P <0.01); 2.8 ± 1.0 vs. 0.8 ± 0.4 in bronchiolar smooth muscle cells ( P <0.05); and 1.1 ± 0.4 vs. 0.2 ± 0.05 in vascular smooth muscle cells ( P <0.05). With FRC-PLV, IL-8/HPRT mRNA expression was significantly lower in macrophages, bronchiolar epithelial, and vascular smooth muscle cells. ICAM-1 mRNA expression in vascular endothelial cells remained unchanged. Predominantly, alveolar macrophages and bronchiolar epithelial cells were involved in the inflammatory pulmonary process. The anti-inflammatory effect of Aerosol-PFC was most pronounced.

15

Gjerdrum, Lise Mette, Ilze Lielpetere, Lars Melholt Rasmussen, Knud Bendix, and Stephen Hamilton-Dutoit. "Laser-Assisted Microdissection of Membrane-Mounted Paraffin Sections for Polymerase Chain Reaction Analysis." Journal of Molecular Diagnostics 3, no. 3 (August 2001): 105–10. http://dx.doi.org/10.1016/s1525-1578(10)60659-9.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

16

Kenngott, R., A. AL-Banaw, M. Vermehren, J. Wendl, and F. Sinowatz. "Application of Laser-Assisted Microdissection for Gene Expression Analysis of Mammalian Germ Cells." Anatomia, Histologia, Embryologia 39, no. 3 (April 23, 2010): 219–26. http://dx.doi.org/10.1111/j.1439-0264.2010.00997.x.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

17

Kwapiszewska, Grazyna, Jai Prakash Muyal, Björn Lengemann, Rainer Bohle, Ludger Fink, Hans Hölschermann, and Sentot Santoso. "Characterization of platelet-specific mRNA by real-time PCR after laser-assisted microdissection." Thrombosis and Haemostasis 90, no. 10 (2003): 749–56. http://dx.doi.org/10.1160/th03-02-0095.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

SummaryCirculating anucleate platelets contain minute amounts of residual megakaryocytic-derived mRNA. To study cell type-specific gene expression in platelets, an accurate and sensitive method to detect and quantify platelet mRNA that excludes contamination with leukocyte RNA is mandatory. Applying laser-assisted microdissection and manipulation (LMM) we could isolate platelets from hemalaun-stained cytospins under permanent visual control and after laser-photolysis of nucleated blood cells. For mRNA quantification, the platelet-specific mRNAs were subsequently measured by real time RT-PCR. High-copy β3 integrin and low-copy α2 integrin as well as tissue factor (TF) transcripts were analyzed in LMM-harvested platelets. In 91.2% (83/91) β3 integrin was detectable with a mean threshold cycle (CT) value of 32.5 ± 3.2 (≤ 50,000 cells). The low-copy α2 integrin mRNA was positive in 84.4% (38/45) with CT mean value of 36.9 ± 1.3, indicating that the relative expression of α2 integrin mRNA in platelets was about 130 times lower than β3.The TF transcript was undetectable in all samples. Comparing platelet mRNA from LMM isolation to that from limiting dilution series resulted in a high accordance for β3 integrin transcript in both, recovery (91.2% vs. 95.2%) and CT value (32.5 vs.32.8).These results demonstrate that the combination of LMM and real-time RT-PCR is a valuable tool for precise, platelet-specific mRNA analysis without contamination of other cells.

18

Philipson, Thomas, Marissa Howard, Kevin Johnson, Amanda Still, Furkat Yunusov, Emanuel Petricoin, Virginia Espina, Noel Gonzalez, Alan Carpino, and Lance Liotta. "Abstract 3768: Optimization of digital pathology through laser capture microdissection with a 405 nm laser." Cancer Research 84, no. 6_Supplement (March 22, 2024): 3768. http://dx.doi.org/10.1158/1538-7445.am2024-3768.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Abstract Tumor cellular heterogeneity is a complex problem in cancer molecular diagnostics and personalized therapy. The tumor is a product of the different types and interactions of host and immune cells. Investigators have created a variety of methods to procure separate subpopulations of the tumor microenvironment for individual analysis. One of the most successful methods of this is laser capture microdissection (LCM). This method captures specific subpopulations of cells under direct microscopic visualization. This LCM technology has successfully been used for over 20 years, revealing a variety of insights into cancer pathogenesis and mechanisms of therapeutic response employed in numerous clinical trials. Nevertheless, there are two drawbacks to the current systems. For UV cutting (365 nm), the tissue is significantly damaged by the UV light energy. Infrared (808 nm) laser systems do not damage the tissue however, this method has low power and poor resolution. Our first hypothesis to be tested is that a new class of near UV 405 nm laser will have small capture areas approaching single cells while simultaneously preventing cellular damage. The second weakness of LCM is the requirement for an open-faced tissue section without a coverslip. Moreover, when visualizing the tissue of interest, the identification of regions of interest is obscured due to the refractive index mismatch between the laser microdissection cap and the tissue surface. We hypothesize that using a volatile organic compound with a refractive index similar to glass would elucidate the tissue region of interest to allow proper visualization. We successfully developed and tested a near 405 nm wavelength LCM system that utilizes a “liquid” coverslip to visualize the sample of interest with higher clarity, resolution, and contrast for immunohistochemistry, immuno-fluorescence, blood smear slides, and conventional hematoxylin-eosin tissue staining. Overall, these critical improvements LCM technology now allow for single-cell tissue capture, system automation, tissue visualization, and opens the door for artificial intelligence-assisted spatial profiling. Citation Format: Thomas Philipson, Marissa Howard, Kevin Johnson, Amanda Still, Furkat Yunusov, Emanuel Petricoin, Virginia Espina, Noel Gonzalez, Alan Carpino, Lance Liotta. Optimization of digital pathology through laser capture microdissection with a 405 nm laser [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 3768.

19

Hernández, Hernán Guillermo, Adrián Gabriel Sandoval-Hernández, Pablo Garrido-Gil, José Luis Labandeira-Garcia, María Victoria Zelaya, Gustavo F. Bayon, Agustín F. Fernández, Mario F. Fraga, Gonzalo Arboleda, and Humberto Arboleda. "Alzheimer's disease DNA methylome of pyramidal layers in frontal cortex: laser-assisted microdissection study." Epigenomics 10, no. 11 (November 2018): 1365–82. http://dx.doi.org/10.2217/epi-2017-0160.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

20

POST, RORY J., ANDREAS KRUGER, and STELLA B. SOMIARI. "Laser-assisted microdissection of polytene chromosomes from Diptera for the development of molecular markers." Molecular Ecology Notes 6, no. 3 (September 2006): 634–37. http://dx.doi.org/10.1111/j.1471-8286.2006.01369.x.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

21

POST, R. J., J. L. CRAINEY, A. BIVAND, and A. RENZ. "Laser-assisted microdissection for the study of the ecology of parasites in their hosts." Molecular Ecology Resources 9, no. 2 (January 31, 2009): 480–86. http://dx.doi.org/10.1111/j.1755-0998.2008.02437.x.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

22

Brauns, Tilmann C., and Manfred Goos. "Micromanipulation of single cells from tissue imprints is an alternative to laser-assisted microdissection." Journal of Cutaneous Pathology 32, no. 7 (August 2005): 461–66. http://dx.doi.org/10.1111/j.0303-6987.2005.00269.x.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

23

Giuffrè, Giuseppe, Annegret Müller, Thomas Brodegger, Tina Bocker-Edmonston, Johannes Gebert, Matthias Kloor, Wolfgang Dietmaier, et al. "Microsatellite Analysis of Hereditary Nonpolyposis Colorectal Cancer-Associated Colorectal Adenomas by Laser-Assisted Microdissection." Journal of Molecular Diagnostics 7, no. 2 (May 2005): 160–70. http://dx.doi.org/10.1016/s1525-1578(10)60542-9.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

24

Saal, Isabelle, Anne Gustin, Katia Rombaut, Pilar Pelaez, Sandrine Rorive, Myriam Remmelink, and Isabelle Salmon. "Laser-assisted microdissection applied to frozen surgical pathologic specimens - methodological aspects on RT-PCR." Journal of Experimental Therapeutics and Oncology 3, no. 6 (November 2003): 325–35. http://dx.doi.org/10.1111/j.1533-869x.2003.01106.x.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

25

Huang, Haibo, Yifan Pan, Yan Pang, Hao Shen, Xiwei Gao, Yichen Zhu, Liguo Chen, and Lining Sun. "Piezoelectric Ultrasonic Biological Microdissection Device Based on a Novel Flexure Mechanism for Suppressing Vibration." Micromachines 12, no. 2 (February 13, 2021): 196. http://dx.doi.org/10.3390/mi12020196.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Biological microdissection has a wide range of applications in the field of molecular pathology. The current laser-assisted dissection technology is expensive. As an economical microdissection method, piezoelectric ultrasonic microdissection has broad application prospects. However, the performance of the current piezoelectric ultrasonic microdissection technology is unsatisfactory. This paper aims to solve the problems of the low dissecting precision and excessive wear of the dissecting needle caused by the harmful lateral vibration of the present piezoelectric ultrasonic microdissection device. A piezoelectric ultrasonic microdissection device based on a novel flexure mechanism is proposed. By analyzing the flexure hinge flexibility, the type of flexure beam and the optimal design parameters are determined. Through harmonic response simulation analysis, the newly designed microdissection device with a vibration-suppressing mechanism achieves the best vibration effect when the driving frequency is 28 kHz. Under this driving frequency, the lateral vibration suppression effect is improved by 68% compared to the traditional effect without vibration suppression. Then, based on 3D printing technology, a prototype of a novel microdissection device is produced, and its performance is tested. Experiments on dissecting needle vibration tests show that the flexure mechanism does indeed suppress the lateral vibration of the needle tip. We conducted various tissue dissection experiments on paraffin tissue sections. First, we determine the optimal dissecting parameters (driving voltage, frequency, feed speed, cutting angle) of the new equipment through various parameter dissecting experiments. Then, we adopt these optimal dissecting parameters to perform three kinds of dissecting experiments on mouse tissue paraffin section (liver, lung, bone), dissecting experiments on tissue sections of different thicknesses (3 μm, 4 μm, 5 μm), sampling and extraction experiments on complete tissue. The new device has a better dissecting performance for paraffin tissue sections below a 5 μm thickness and can complete various dissecting tasks. Finally, we compare the wear of the dissecting needles of the new and old devices after the same dissecting tasks. The results prove that the suppression of harmful lateral vibration not only significantly improves the dissecting effect but also increases the service life and durability of the dissecting needle, which is beneficial for reducing the equipment costs.

26

Klink, Vincent P., Giselle Thibaudeau, and Ronald Altig. "A Novel Sample Preparation Method That Enables Nucleic Acid Analysis from Ultrathin Sections." Microscopy and Microanalysis 19, no. 3 (March 21, 2013): 635–41. http://dx.doi.org/10.1017/s1431927613000044.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

AbstractThe ability to isolate and perform nucleic acid analyses of individual cells is critical to studying the development of various cell types and structures. We present a novel biological sample preparation method developed for laser capture microdissection-assisted nucleic acid analysis of ultrathin cell/tissue sections. We used cells of the mitotic bed of the tadpole teeth of Lithobates sphenocephalus (Southern Leopard Frog). Cells from the mitotic beds at the base of the developing teeth series were isolated and embedded in the methacrylate resin, Technovit® 9100®. Intact cells of the mitotic beds were thin sectioned and examined by bright-field and transmission electron microscopy. The cytological and ultrastructural anatomy of the immature and progressively more mature tooth primordia appeared well preserved and intact. A developmental series of tooth primordia were isolated by laser capture microdissection (LCM). Processing of these cells for RNA showed that intact RNA could be isolated. The study demonstrates that Technovit® 9100® can be used as an embedding medium for extremely small tissues and from individual cells, a prerequisite step to LCM and nucleic acid analyses. A relatively small amount of sample material was needed for the analysis, which makes this technique ideal for cell-specific analyses when the desired cells are limited in quantity.

27

Alevizos, Ilias, Mamatha Mahadevappa, Xue Zhang, Hiroe Ohyama, Yohko Kohno, Marshall Posner, George T. Gallagher, et al. "Oral cancer in vivo gene expression profiling assisted by laser capture microdissection and microarray analysis." Oncogene 20, no. 43 (September 2001): 6196–204. http://dx.doi.org/10.1038/sj.onc.1204685.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

28

Burnet, Philip W. J., Sharon L. Eastwood, and Paul J. Harrison. "Laser-assisted microdissection: methods for the molecular analysis of psychiatric disorders at a cellular resolution." Biological Psychiatry 55, no. 2 (January 2004): 107–11. http://dx.doi.org/10.1016/s0006-3223(03)00642-5.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

29

Nakanishi, Yoko, Gou Mizutani, Makoto Sano, Toshinori Oinuma, and Norimichi Nemoto. "Comparison of HER2 mRNA Amplification with Immunohistochemistry in Human Breast Cancer Using Laser Assisted Microdissection Technique." ACTA HISTOCHEMICA ET CYTOCHEMICA 37, no. 2 (2004): 73–79. http://dx.doi.org/10.1267/ahc.37.73.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

30

Santi, Simonetta, and Wolfgang Schmidt. "Laser microdissection-assisted analysis of the functional fate of iron deficiency-induced root hairs in cucumber." Journal of Experimental Botany 59, no. 3 (February 2008): 697–704. http://dx.doi.org/10.1093/jxb/erm351.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

31

Peiser, Christian, Marcello Trevisani, David A. Groneberg, Q. Thai Dinh, Doerthe Lencer, Silvia Amadesi, Barbara Maggiore, Selena Harrison, Pierangelo Geppetti, and Axel Fischer. "Dopamine type 2 receptor expression and function in rodent sensory neurons projecting to the airways." American Journal of Physiology-Lung Cellular and Molecular Physiology 289, no. 1 (July 2005): L153—L158. http://dx.doi.org/10.1152/ajplung.00222.2004.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Agonists of the dopamine receptors have been demonstrated to have bronchodilatory properties in pathologically constricted airways. The mechanism by which these agonists induce bronchodilatation is thought to involve airway sensory nerves. In this study, the expression and function of dopamine D2 receptor were examined in sensory ganglia supplying the airways. Neuronal dopamine D2 receptor mRNA expression was demonstrated by single-cell RT-PCR following laser-assisted microdissection. The projection of the neurons to the airways was confirmed by retrograde neuronal labeling. In functional studies, dopamine D2 receptor agonists (AR-C65116AB and ropinirole) inhibited intraneuronal calcium mobilization in rat capsaicin-sensitive primary sensory neurons and capsaicin-induced plasma extravasation in the rat trachea. Our results provide support to the hypothesis that dopamine D2 receptor activation inhibits neurogenic inflammation and proinflammatory reflex responses.

32

Iida, Yuko, Yasuhiro Gon, Yoko Nakanishi, Yusuke Kurosawa, Yoshiko Nakagawa, Kenji Mizumura, Tetsuo Shimizu, Noriaki Takahashi, and Shinobu Masuda. "Genomic analysis between idiopathic pulmonary fibrosis and associated lung cancer using laser‐assisted microdissection: A case report." Thoracic Cancer 12, no. 9 (March 30, 2021): 1449–52. http://dx.doi.org/10.1111/1759-7714.13924.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

33

Nagai-Okatani, Chiaki, Misugi Nagai, Takashi Sato, and Atsushi Kuno. "An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection." International Journal of Molecular Sciences 20, no. 3 (February 6, 2019): 700. http://dx.doi.org/10.3390/ijms20030700.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Lectin microarray (LMA) is a highly sensitive technology used to obtain the global glycomic profiles of endogenous glycoproteins in biological samples including formalin-fixed paraffin-embedded tissue sections. Here, we describe an effective method for cell type-selective glycomic profiling of tissue fragments collected by laser microdissection (LMD) under fluorescent histochemical visualization. We optimized each step of histochemical staining and confirmed the reliability and validity of glycomic profiling. Using the optimized procedure, glycomic profiles were obtained with 0.5 mm2 of stained thymic sections (5-μm-thick) from 8-week-old C57BL/6J male mice. The glycomic profiles of Ulex europaeus agglutinin-I (UEA-I)-stained medullary regions showed higher UEA-I signals than those of the morphologically determined medulla regions, indicating the utility of this method for UEA-I(+) cell-selective analysis. To further evaluate this method, tissue fragments was serially collected from stained and unstained areas of medullary epithelial cell probes (UEA-I and anti-cytokeratin 5 antibody) and a cortex-staining probe (peanut agglutinin). The medullary regions assigned by the three probes showed significantly different glycomic profiles, highlighting the difference in subpopulation recognition among the three probes, which was consistent with previous reports. In conclusion, our fluorescence LMD-LMA method enabled cell type-selective tissue glycomic analysis of pathological specimens and animal models, especially for glyco-biomarker discovery.

34

Selmaj, Krzysztof, Zofia Pawłowska, Agata Walczak, Wiktor Koziołkiewicz, Cedric S. Raine, and Czesław S. Cierniewski. "Corpora amylacea from multiple sclerosis brain tissue consists of aggregated neuronal cells." Acta Biochimica Polonica 55, no. 1 (February 5, 2008): 43–50. http://dx.doi.org/10.18388/abp.2008_3199.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

In this report, we describe proteomic analysis of corpora amylacea collected by postmortem laser microdissection from multiple sclerosis (MS) brain lesions. Using low level protein loads (about 30 microg), a combination of two-dimensional electrophoresis with matrix-assisted laser desorption/ionization-time of flight mass spectrometry and database interrogations we identified 24 proteins of suspected neuronal origin. In addition to major cytoskeletal proteins like actin, tubulin, and vimentin, we identified a variety of proteins implicated specifically in cellular motility and plasticity (F-actin capping protein), regulation of apoptosis and senescence (tumor rejection antigen-1, heat shock proteins, valosin-containing protein, and ubiquitin-activating enzyme E1), and enzymatic pathways (glyceraldehyde-3-dehydrogenase, protein disulfide isomerase, protein disulfide isomerase related protein 5, lactate dehydrogenase). Samples taken from regions in the vicinity of corpora amylacea showed only traces of cellular proteins suggesting that these bodies may represent remnants of neuronal aggregates with highly polymerized cytoskeletal material. Our data provide evidence supporting the concept that biogenesis of corpora amylacea involves degeneration and aggregation of cells of neuronal origin.

35

Schmid, Marc W., Anja Schmidt, Ulrich C. Klostermeier, Matthias Barann, Philip Rosenstiel, and Ueli Grossniklaus. "A Powerful Method for Transcriptional Profiling of Specific Cell Types in Eukaryotes: Laser-Assisted Microdissection and RNA Sequencing." PLoS ONE 7, no. 1 (January 26, 2012): e29685. http://dx.doi.org/10.1371/journal.pone.0029685.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

36

Kleeberger, Wolfram, Thomas Rothämel, Sabine Glöckner, Ulrich Lehmann, and Hans Kreipe. "Laser-Assisted Microdissection and Short Tandem Repeat PCR for the Investigation of Graft Chimerism after Solid Organ Transplantation." Pathobiology 68, no. 4-5 (2000): 196–201. http://dx.doi.org/10.1159/000055923.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

37

TIAN, XUE-FEI, XUE-GONG FAN, YAN ZHANG, YAN HUANG, HONG DAI, and RUO-SU YING. "Procuration and identification of bacteria in paraffin-embedded liver tissues of hepatocellular carcinoma by laser-assisted microdissection technique." APMIS 116, no. 1 (January 2008): 10–15. http://dx.doi.org/10.1111/j.1600-0463.2008.00739.x.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

38

Tømmerås, Karin, Ingunn Bakke, Arne K. Sandvik, Erik Larsson, and Helge L. Waldum. "Rat parietal cells express CCK2 receptor mRNA: gene expression analysis of single cells isolated by laser-assisted microdissection." Biochemical and Biophysical Research Communications 297, no. 2 (September 2002): 335–40. http://dx.doi.org/10.1016/s0006-291x(02)02202-7.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

39

Pinzani, P., C. Orlando, and M. Pazzagli. "Corrigendum to “Laser-assisted microdissection for real-time PCR sample preparation” [JMAM 27 (2–3) (2006) 140–159]." Molecular Aspects of Medicine 29, no. 3 (June 2008): 201. http://dx.doi.org/10.1016/j.mam.2006.11.001.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

40

Gautam, Vibhav, and Ananda K. Sarkar. "Laser Assisted Microdissection, an Efficient Technique to Understand Tissue Specific Gene Expression Patterns and Functional Genomics in Plants." Molecular Biotechnology 57, no. 4 (November 18, 2014): 299–308. http://dx.doi.org/10.1007/s12033-014-9824-3.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

41

Kenngott, R., G. Palma, M. J. Wendl, M. Vermehren, and F. Sinowatz. "213 ANALYSIS OF STEROID HORMONE RECEPTOR GENE EXPRESSION IN THE BOVINE OVARY AFTER LASER-CAPTURE MICRODISSECTION." Reproduction, Fertility and Development 22, no. 1 (2010): 264. http://dx.doi.org/10.1071/rdv22n1ab213.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Developmental processes in complex organs like the ovary are difficult to study in terms of a biochemical and molecular biological analysis. Laser-assisted microdissection allows the efficient and precise capture of single cells or groups of cells of an organ within the context of time and space and permits their subsequent molecular characterization. Together with real-time PCR techniques, it is now feasible to study gene expression in defined cell populations of complex tissues, but it is essential to create standards optimized for fixation, preparation, and isolation of RNA, reverse transcription reaction, and real-time PCR protocol for every tissue of interest. The aim of our study was to develop protocols for a precise analysis of estrogen receptor alpha (ER-α) and progesterone receptor (PR) in defined compartments of the ovary (granulosa cells, theca interna cells, zona vasculosa, and zona parenchymatosa of the stroma). Additionally, the receptor proteins were localized by immunohistochemistry. A special focus was put on the question of how formalin fixation and paraffin embedding influences the quality of the isolated RNA from microdissected material, which was used for quantitative reverse transcription-PCR (qRT-PCR). Quality and quantity of total RNA extracted from formalin-fixed, paraffin-embedded (FFPE) sections and from material immersed in RNAlater® (Ambion, Foster City, CA, USA) was checked using an Experion automated electrophoresis system (Bio-Rad, Munich, Germany). The RNA quality indicator for microdissected material was between 6 and 7, and for RNAlater® material was 9 or better. Online qRT-PCR using the iCycler SYBR GreenTM protocol (Bio-Rad) was performed in a 96-well plate. Primer pairs were chosen to generate PCR products between 100 bp (ER-α) and 140 bp (PR), as RNA recovered from FFPE-laser microdissected material was expected to be considerably fragmented. Using GenEx software (BioEPS, Freisling, Germany), we showed that the expression of mRNA for PR was much stronger in the theca interna than in the 3 other compartments. Estrogen receptor alpha, on the other hand, was nearly exclusively expressed in the zona parenchymatosa and zona vasculosa of the stroma. Our results show that cells obtained after laser microdissection from FFPE ovarian material can be successfully used for subsequent real-time PCR, despite the fact the RNA quality indicator number of the isolated RNA was usually comparatively low. The data of our immunohistochemical analysis support the expression data of our RNA studies. In conclusion, laser-capture microdissection in combination with quantitative PCR is a reproducible and reliable technique for quantification of a small number of cells from FFPE material. We gratefully acknowledge the continuous support by the DFG-Graduiertenkolleg 1029 and the BMBF (ARG 08/013).

42

Himmel, Lauren E., Troy A. Hackett, Jessica L. Moore, Wilson R. Adams, Giju Thomas, Tatiana Novitskaya, Richard M. Caprioli, Andries Zijlstra, Anita Mahadevan-Jansen, and Kelli L. Boyd. "Beyond the H&E: Advanced Technologies for in situ Tissue Biomarker Imaging." ILAR Journal 59, no. 1 (2018): 51–65. http://dx.doi.org/10.1093/ilar/ily004.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

AbstractFor decades, histopathology with routine hematoxylin and eosin staining has been and remains the gold standard for reaching a morphologic diagnosis in tissue samples from humans and veterinary species. However, within the past decade, there has been exponential growth in advanced techniques for in situ tissue biomarker imaging that bridge the divide between anatomic and molecular pathology. It is now possible to simultaneously observe localization and expression magnitude of multiple protein, nucleic acid, and molecular targets in tissue sections and apply machine learning to synthesize vast, image-derived datasets. As these technologies become more sophisticated and widely available, a team-science approach involving subspecialists with medical, engineering, and physics backgrounds is critical to upholding quality and validity in studies generating these data. The purpose of this manuscript is to detail the scientific premise, tools and training, quality control, and data collection and analysis considerations needed for the most prominent advanced imaging technologies currently applied in tissue sections: immunofluorescence, in situ hybridization, laser capture microdissection, matrix-assisted laser desorption ionization imaging mass spectrometry, and spectroscopic/optical methods. We conclude with a brief overview of future directions for ex vivo and in vivo imaging techniques.

43

Glöckner, Sabine, Ulrich Lehmann, Nadine Wilke, Wolfram Kleeberger, Florian Länger, and Hans Kreipe. "Detection of Gene Amplification in Intraductal and Infiltrating Breast Cancer by Laser-Assisted Microdissection and Quantitative Real-Time PCR." Pathobiology 68, no. 4-5 (2000): 173–79. http://dx.doi.org/10.1159/000055920.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

44

Ruzicka, W. B., A. Zhubi, M. Veldic, D. R. Grayson, E. Costa, and A. Guidotti. "Selective epigenetic alteration of layer I GABAergic neurons isolated from prefrontal cortex of schizophrenia patients using laser-assisted microdissection." Molecular Psychiatry 12, no. 4 (January 30, 2007): 385–97. http://dx.doi.org/10.1038/sj.mp.4001954.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

45

null, null. "P8-03: Exact quantification of gene amplification in archival tissue sections by laser-assisted microdissection and real-time PCR." Breast Cancer Research 2, Suppl 1 (2000): S41. http://dx.doi.org/10.1186/preaccept-158393212.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

46

O'Brien, Philippa M., David W. M. Millan, Jonathon A. Davis, and M. Saveria Campo. "In Situ Isolation of Immunoglobulin Sequences Expressed by Single Tumor-Infiltrating B Cells Using Laser-Assisted Microdissection." Molecular Biotechnology 29, no. 2 (2005): 101–10. http://dx.doi.org/10.1385/mb:29:2:101.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

47

Aubele, Michaela, Horst Zitzelsberger, Ulrich Schenck, Axel Walch, Heinz H�fler, and Martin Werner. "Distinct cytogenetic alterations in squamous intraepithelial lesions of the cervix revealed by laser-assisted microdissection and comparative genomic hybridization." Cancer 84, no. 6 (December 25, 1998): 375–79. http://dx.doi.org/10.1002/(sici)1097-0142(19981225)84:6<375::aid-cncr10>3.0.co;2-1.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

48

Fede, Jacquelyn, Stephen Kogut, Anthony Heyward, John F. Stevenson, Amy Nunn, Julie Plaut, and Judy A. Kimberly. "21746 Antigen discovery in membranous glomerulopathy using laser capture microdissection and mass spectrometry." Journal of Clinical and Translational Science 5, s1 (March 2021): 1–2. http://dx.doi.org/10.1017/cts.2021.405.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

ABSTRACT IMPACT: Identifying the causative antigen in membranous glomerulopathy cohorts enables the development of serum assays to detect and monitor disease progression without the need for invasive kidney biopsies. OBJECTIVES/GOALS: Primary membranous glomerulopathy is caused by the formation of autoantibody immune complexes which deposit in the glomerulus and obstruct kidney function. Causative antigens remain to be identified in roughly 20% of cases. Our goal is to identify the antigen in these cohorts, so that non-invasive assays can be developed for disease monitoring. METHODS/STUDY POPULATION: Renal biopsy tissue from known antigen cases (PLA2R, THSD7A), and unknown cases were included in the analysis. Renal biopsy tissue from formalin fixed paraffin embedded tissue was cut at a thickness of 10 µm onto Leica PET-membrane frame slides. These slides were then stained with hematoxylin. The glomeruli were microdissected into microcentrifuge tubes using a Leica DM6000B microscope. The microdissected glomeruli were lysed in 2% SDS and 0.1M DTT at 99 degrees Celsius for 1 hour and processed by filter assisted sample preparation (FASP). Digested peptides were analyzed by liquid chromatography-mass spectrometry using an Orbitrap Fusion Lumos using data-dependent acquisition. RESULTS/ANTICIPATED RESULTS: Mass spectrometry data collected from the laser captured glomeruli was searched against the human proteome fasta database from Uniprot using MaxQuant. IBAQ values were used for quantitation and statistical analysis. Null hypothesis significance testing was performed for each protein by comparing each sample group to the rest of the samples in the data set. In the control groups, the causative antigens PLA2R and THSD7A were detected and quantified with the largest magnitude fold change in their respective category, validating the experimental design. Using this approach, the proteins SAP, NELL1, and NCAM1 were identified and subsequently validated as causative antigens in distinct patient cohorts. DISCUSSION/SIGNIFICANCE OF FINDINGS: Here, we share the results of our efforts to comprehensively identify the spectrum of causative antigens in membranous glomerulopathy. In this context, antigen discovery is an essential first step for the development of non-invasive assays to inform prognosis, monitor response to treatment, and better understand disease etiology.

49

Miller, R. Lance, Ping Zhang, Maren Smith, Valerie Beaulieu, Teodor G. Păunescu, Dennis Brown, Sylvie Breton, and Raoul D. Nelson. "V-ATPase B1-subunit promoter drives expression of EGFP in intercalated cells of kidney, clear cells of epididymis and airway cells of lung in transgenic mice." American Journal of Physiology-Cell Physiology 288, no. 5 (May 2005): C1134—C1144. http://dx.doi.org/10.1152/ajpcell.00084.2004.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

The kidney, epididymis, and lungs are complex organs with considerable epithelial cell heterogeneity. This has limited the characterization of pathophysiological transport processes that are specific for each cell type in these epithelia. The purpose of the present study was to develop new tools to study cell-specific gene and protein expression in such complex tissues and organs. We report the production of a transgenic mouse that expresses enhanced green fluorescent protein (EGFP) in a subset of epithelial cells that express the B1 subunit of vacuolar H+-ATPase (V-ATPase) and are actively involved in proton transport. A 6.5-kb portion of the V-ATPase B1 promoter was used to drive expression of EGFP. In two founders, quantitative real-time RT-PCR demonstrated expression of EGFP in kidney, epididymis, and lung. Immunofluorescence labeling using antibodies against the B1 and E subunits of V-ATPase and against carbonic anhydrase type II (CAII) revealed specific EGFP expression in all renal type A and type B intercalated cells, some renal connecting tubule cells, all epididymal narrow and clear cells, and some nonciliated airway epithelial cells. No EGFP expression was detected in collecting duct principal cells (identified using an anti-AQP2 antibody) or epididymal principal cells (negative for V-ATPase or CAII). This EGFP-expressing mouse model should prove useful in future studies of gene and protein expression and their physiological and/or developmental regulation in distinct cell types that can now be separated using fluorescence-assisted microdissection, fluorescence-activated cell sorting, and laser capture microdissection.

50

Lehmann, Ulrich, Sabine Glöckner, Wolfram Kleeberger, Henning Feist Reinhard von Wasielewski, and Hans Kreipe. "Detection of Gene Amplification in Archival Breast Cancer Specimens by Laser-Assisted Microdissection and Quantitative Real-Time Polymerase Chain Reaction." American Journal of Pathology 156, no. 6 (June 2000): 1855–64. http://dx.doi.org/10.1016/s0002-9440(10)65059-6.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.