Дисертації з теми "Large-Scale Screening"
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García, Martín Rafael Adrián, and Sánchez José Manuel Gaspar. "Screening for important factors in large-scale simulation models: some industrial experiments." Thesis, Högskolan i Skövde, Institutionen för ingenjörsvetenskap, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-11484.
Повний текст джерелаZein, Aghaji Mohammad. "Large Scale Computational Screening of Metal Organic Framework Materials for Natural Gas Purification." Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/36226.
Повний текст джерелаFalk, Jessica Julia [Verfasser]. "Large-scale screening of blood donors for exceptional antibodies against human cytomegalovirus / Jessica Julia Falk." Ulm : Universität Ulm, 2019. http://d-nb.info/1184429855/34.
Повний текст джерелаKlisa, Christiane. "Identification and characterisation of novel zebrafish brain development mutants obtained by large scale forward mutagenesis screening." Doctoral thesis, [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=970092202.
Повний текст джерелаFaust, Doerte [Verfasser]. "Identification of proteins controlling AQP2 translocation by large-scale siRNA screening of the mouse kinome / Doerte Faust." Berlin : Freie Universität Berlin, 2014. http://d-nb.info/1054328897/34.
Повний текст джерелаRose, France. "Analysis of phenotypic and spatial cellular heterogeneity from large scale microscopy data." Electronic Thesis or Diss., Paris Sciences et Lettres (ComUE), 2019. http://www.theses.fr/2019PSLEE057.
Повний текст джерелаRobotics and automated fluorescence microscopes have promoted high-content cell-based screenings: fluorescent probes targeting DNA or other major components are used to image hundreds of thousands of cells under many different conditions. Cell-based assays have proven to be efficient at discovering first-in-class therapeutic drugs, i.e. drugs acting on a new target. They allow to detect promising molecules and to profile them, by associating functional annotations to them, like their molecular target or mechanism of action (MOA). I studied heterogeneity of cell responses at different levels and how this phenotypic heterogeneity can be leveraged to better profile drugs. The first level is about studying heterogeneity between patients. We showed that using different patient-derived cell lines increases the chance of predicting the correct molecular target of the tested drug. The second level corresponds to the diversity of cell responses within the same cell line under the same treatment. Appropriate clustering approaches can be used to unravel this complexity and group cells into subpopulations. The proportions of each subpopulation per treatment allow to predict the correct MOA. The third level looks at how the cell subpopulations are spatially organized. I found that neighboring cells influence each others, and display a similar phenotype more frequently than expected at random. These results assessed across a hundred of treatments, show that even genetically identical cells are not all alike and independent, but create spatial heterogeneity via cell lineage and interaction. Using spatial information as well as phenotypic heterogeneity with graph kernel methods improves the MOA classification under some conditions. Alongside, as spatial analysis could be applied on any cell microscopy image, I developed a Python analysis package, pySpacell, to study spatial randomness from quantitative and qualitative cell markers
Aaramaa, S. (Sanja). "Developing a requirements architecting method for the requirement screening process in the Very Large-Scale Requirements Engineering Context." Doctoral thesis, Oulun yliopisto, 2017. http://urn.fi/urn:isbn:9789526217079.
Повний текст джерелаTiivistelmä Tutkimus toteutettiin laajamittaisen vaatimusmäärittelyprosessin kontekstissa keskittyen vaatimusten seulontaprosessiin. Vaatimusten seulontaprosessi määritellään tuotekehityksen alkuvaiheen prosessiksi, jossa käsitellään jatkuvana vuona tulevia kehityspyyntöjä. Vaatimusten seulontaprosessissa pyritään tunnistamaan tehokkaasti lupaavimmat pyynnöt jatkoanalyysiä, tuotekehitystä ja toteutusta ajatellen sekä suodattamaan pois niin aikaisessa vaiheessa, kun mahdollista ne pyynnöt, joilla ei ole arvontuotto-odotuksia. Tutkimuksen tavoite oli ymmärtää haasteita, jotka liittyvät vaatimusten seulontaprosessiin sekä kehittää ratkaisuja näihin haasteisiin. Tutkimuksessa käytettiin laadullisen tutkimuksen menetelmiä. Kokonaisuutena tutkimusprosessi noudattaa toimintatutkimuksen periaatteita siten, että jokainen sykli tai sen vaihe sisältää yhden tai useamman itsenäisesti määritellyn tapaustutkimuksen suunnittelun ja läpiviennin. Valitut tutkimusmenetelmät soveltuvat hyvin tilanteisiin, joissa tutkimuskohteina ovat reaalimaailman ilmiöt niiden luonnollisissa ympäristöissä havainnoituina. Tutkimusaineisto kerättiin kahdesta informaatio- ja kommunikaatioteknologia-alan kohdeorganisaatiosta. Väitöskirjaan sisällytettyihin julkaisuihin I-V on analysoitu 45 haastattelun aineisto. Näiden lisäksi väitöskirjassa kuvatun pitkäkestoisen toimintatutkimuksen aikana hyödynnettiin 26 haastattelun ja 132 työpajan aineistoa kehitettäessä ratkaisuja vaatimusten seulontaprosessin haasteisiin. Vaatimusten seulontaprosessi on laajamittaisen vaatimusmäärittelyprosessin teollinen toteutus. Tutkimuksessa tunnistettiin useita merkittäviä haasteta, joita eri sidosryhmillä on liittyen vaatimusten seulontaprosessiin ja päätöksentekoon laajamittaisessa vaatimusmäärittelyprosessissa. Vaatimusten suuri määrä, päätöksentekoon tarvittavan tiedon puute ja käytössä olevien työkalujen soveltumattomuus ovat esimerkkejä tunnistetuista haasteista. Ratkaisuna haasteisiin kehitettiin vaatimusten seulonta- ja analyysimenetelmä. Kehitetty menetelmä sisältää dynaamisen vaatimusdokumentin, jonka avulla voidaan kerätä kehityspyyntöjen tietosisältö jäsennellysti, dokumentoida ja kommunikoida vaatimukset sekä muodostaa niistä tuotteisiin toteutettavia ominaisuuksia ottaen huomioon eri sidosryhmien tarpeet. Kehitetty menetelmä on koestettu, validoitu ja soveltuvin osin otettu käyttöön teollisuudessa
Niranjane, Ajay Pundaiikrao, and ajay niranjane@gmail com. "Screening diverse cellulase enzymes from the white rot fungus Phlebia gigantea for high activity and large scale applications." RMIT University. Applied Sciences, 2006. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080513.150257.
Повний текст джерелаZhang, Hanshuo. "Large-scale identification of functional genes regulating cancer cell migration and metastasis using the self-assembled cell microarray." Diss., Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/49066.
Повний текст джерелаChen, Peng-yu, and 陳芃妤. "Large Scale Screening of Plasticizers by Ambient Mass Spectrometry." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/96cv84.
Повний текст джерела國立中山大學
化學系研究所
103
Ambient mass spectrometry (AMS) is known for its unique feature to perform analysis without sample pretreatment, and has been used for direct, rapid, and real-time detection of chemical compounds. Techniques such as DESI, DART, and ELDI have been demonstrated to be useful for rapidly characterizing chemical and biological compounds. In this study, we have developed an ambient mass spectrometric technique known as thermal desorption electrospray ionization mass spectrometry (TD-ESI/MS). A direct metallic sampling probe was used to collect analytes from sample surfaces regardless of sample size or shape. Analytes were thermally desorbed, post-ionized by reacting with charged solvent species in an electrospray plume, and the ions were subsequently detected by the mass analyzer attached to the ion source. .The residual sample on the metallic probe is rapidly removed by burning the probe with a flame from a torch. The time required to complete an analysis was less than 15 seconds. In the first study, TD-ESI/MS was used to screen phthalates [ Dibutyl Phthalate (DBP), Dimethyl Phthalate (DMP), Di-octyl Phthalate (DOP), Di(2-ethylhexyl)Phthalate (DEHP), Di-iso-nonyl Phthalate (DINP), Benzyl Butyl Phthalate (BBP), Di-isodecyl Phthalate (DIDP)and Diethyl Phthalate (DEP) ] on the objects in two kindergartens. Approximately one thousand samples were collected, analyzed and the results were reported within two days. Sample collection was completed in approximately 3hrs, TD-ESI/MS analysis was completed in 10 hrs, and data organization and report writing took another 5 hrs. The results indicate that approximately 30% and 20% of the objects in the two kindergartens contain higher level of phthalates. The success in screening phthalates in all the objects found in the two kindergartens suggests that performing large scale screening of phthalates in the living environment is possible. In the second study, liquid-phase microextraction (LPME) was coupled with an ambient ionization technique, known as thermal desorption electrospray ionization (TD-ESI) for rapid screening of veterinary drug residues in foods. The ambient TD-ESI ion source consisted of (1)a metal loop suspending 5 μL of organic solvent was used for analyte extraction in liquids, (2)after equilibrium among analytes, sample solution, and extraction solvent was reached, a heating oven for desorbing analytes on the LPME probe.Preliminary results of LPME/TD-ESI/MS/ showed that reproducibility tests (n=5) for 10 µg/mL of sulfonamides(sulfamethazine, sulfamethoxazole) and β-agonists (clenbuterol, salbutamol, terbutaline) were less than 7.6 % and 10.2 %, respectively. The sensitivities of sulfamethazine(m/z 279) and clenbuterol(m/z 277)improved,; where detection limits could be decreased as low as 1.0 µg/mL, and 0.1 µg/mL while using the LPME fiber as a sampling probe. Furthermore, the capacity of LPME/TD-ESI/MS for quantitative analysis was evaluated using milk, honey and pork blood spiked with spiked veterinary drugs. Excellent correlations of determination were achieved for analytes with R2values greater than 0.997、0.998 and 0.972, respectively.
BRIESTENSKÁ, Petra. "Large scale screening of microalgae and cyanobacteria for a presence of bioactive compounds." Doctoral thesis, 2007. http://www.nusl.cz/ntk/nusl-85455.
Повний текст джерелаYANG, CHIAN-YI, and 楊千儀. "By A Large Scale Behavioral Screening to Verify Specific Micro-RNAs that Affected Drosophila Courtship Responses." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/79325313695142769446.
Повний текст джерела國立暨南國際大學
應用化學系
104
Micro-RNAs (miRNAs) are small non-coding RNAthat only need to pair partially to the target mRNA in order to elicit translational repression.Previously, our lab used behavioral screening to define 11 distinct dm-miRNAs from the collection of 160 UAS-miRNA lines that drove expression in elav-expressing (pan-neuronal) cells of adult Drosophila males, which can prompt inter-male courtship behavior.In my study, the underling same experimental approachto find outthe role of the individual miRNA involves the male or female fly heterosexualcourtship activity. Our result showed that 15 miRNAs affected male courtship responses and 36 miRNAs affected female courtship responses.We highlight the importance of these miRNAs in neuronal system, and expect to identify the miRNAs targets and gain molecular insights in the mechanisms of fly courtship activity.Our long-term goal is todissect sex processes at biochemical, genetic, anatomical, and behavioral levels. Such “translational” studies of the flysystems neuroscience generate clinically relevant insight into human brain function and ultimately may lead to important advances in the understanding in vertebrate.
Reed, Eric R. "Development of advanced methods for large-scale transcriptomic profiling and application to screening of metabolism disrupting compounds." Thesis, 2020. https://hdl.handle.net/2144/41943.
Повний текст джерелаKlisa, Christiane [Verfasser]. "Identification and characterisation of novel zebrafish brain development mutants obtained by large scale forward mutagenesis screening / von Christiane Klisa." 2003. http://d-nb.info/970092202/34.
Повний текст джерелаHuang, Hsin-Mei, and 黃心玫. "Exploration of the type VI secretion system regulators in Azorhizobium caulinodans ORS571 by large-scale transposon-based mutagenesis and screening." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/hmcth5.
Повний текст джерела國立臺灣大學
生物科技研究所
105
Type VI secretion system (T6SS) is molecular machine that is widespread among phylum Proteobacteria to mediate interactions with neighboring eukaryotic and prokaryotic cells. The legume symbiont, Azorhizobium caulinodans ORS571 (α-proteobacteria), possess one deduced T6SS cluster harboring impA~impL genes on the genome. However, the T6SS hallmark protein, Hcp (hemolysin-coregulated protein) is not detectable in the extracellular matrix of ORS571 under current culture conditions. To facilitate the study of the role and regulatory pathway of T6SS in ORS571 life cycle, I screened for T6SS-active mutants from a transposon mutant library using colony lift immunoassay. The genome-wide mutant library was constructed by conjugative transfer of mini-Tn5 vector (pFAJ1819) into ORS571 cells followed by irreversible random Tn5 insertions into the genome. Efficiency of the transposon-based mutagenesis was increased 3~20 fold by refrigerating (3~11oC) the post-conjugation suspension for 2 days ~ 4 weeks before spreading on antibiotic selection plates. The improved mutation efficiency enabled colony development at high density, and these mutant colonies were directly transferred onto membrane and incubated overnight for accumulation of secreted Hcp protein. Background noise caused by adherent colonies was effectively reduced by using the dispersing chemical NaIO4 followed by a thorough vortex step. Accordingly, the detection limit was improved down to 6.3 ng Hcp protein. Approximately 68,000 mutants were analyzed, and 4 Hcp-secreting mutants and 1 Hcp-overexpression mutant were identified. Among the 4 Hcp-secreting mutants, strain Tn-A3 displayed decreased viability and imp-independent Hcp release caused by overexpression of phage-related loci (Azc_2137-2139); strain Tn-A2 and Tn-h91 showed compromised vitality and imp-independent Hcp secretion/release caused by C-terminal disruption of alanine racemase (alr, Azc_2065); strain Tn-A4 was disrupted in hypothetical gene Azc_0044, so it was pending further investigation. On the other hand, Hcp-overexpression strain Tn-A1 was unexpectedly identified by its phenotype of hyper-adherence presumably due to phosphate dyshomeostasis (pstS, Azc_4037). Further genetic verifications are required to assert the regulatory role of phosphate homeostasis on T6SS. Overall, the near-saturation screen suggests pathway(s) other than the deduced T6SS (Azc_2586-2605) participating in Hcp secretion/ release in A. caulinodans ORS571, and the mechanism(s) await future elucidation.
Su, Yi-Ju, and 蘇奕如. "By a large scale behavioral screening to verify specific microRNAs and microRNA target genes that affected Drosophila male-male courtship behavior." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/92109337719797134318.
Повний текст джерела國立暨南國際大學
應用化學系
103
Courtship, an instinct of animals in the nature, normally occurs in between opposite sexes. Nevertheless, a lot of evidences proved that courtship and sexual behaviors existed between the same sexes in many species. It involved cellular or molecular mechanism is poorly understood. Drosophila melanogaster as an idea model provides advanced genetic tools for the neuronal circuit manipulations combined with sophispicated animal behaviors to realize the physiological mechanism underlying the homo-sexual behavior. We used behavioral screening to define 11 distinct dm-miRNAs (mir-10, -11, -34, -124, -252, -276b, -984, -985, -987, -989 and -1012) from the collection of 160 UAS-dme-miRNA lines that drove expression in elav-expressing cells of adult Drosophila males, which can prompt inter-male courtship behavior. Some of miRNA targets were also further predicted using some of miRNA target prediction resources. It should be noted that many common target genes, and some targets associated with same signaling pathway was predicted from 11 miRNAs. Therefore, it is of particular interest to reliably these predict potential miRNA targets which might be involved in this phenomenon. Finally, our data further confirm that 3 miRNAs (miRNA-10, -252 and -987) have a significant impact on the mRNA levels of porcupine (por) and demonstrate that down-regulate por level in adult male fly’ neuronal system can also induce the inter-male courtship behavior. An evolutionary perspective of homo-sexual that incorporates mechanisms related in flies, may lead to important advances in the understanding targeting molecules in human males.
Ngonyamo-Majee, Dainah. "Evaluation of corn endosperm properties and development of near infrared reflectance spectroscopy (NIRS) calibrations for screening germplasm on starch digestibility in large scale corn breeding programs." 2005. http://catalog.hathitrust.org/api/volumes/oclc/65642944.html.
Повний текст джерелаLu, Ying-Hsuan, and 呂盈暄. "Large-Scale Quantile-based Simulation Optimization Using Efficient Factor Screenings." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/v92but.
Повний текст джерела國立清華大學
工業工程與工程管理學系所
105
Screening experiments are often conducted before optimization in order to reduce computation resources by identifying the important factors of the problem. In the literatures, factor screening and simulation optimization approaches mostly adopted expectation as performance measures. The methodologies that are focused on other alternatives, however, are difficult to develop due to a lack of nice statistical properties as expectation. Quantile is an important alternative to the expectation for spatial data and moreover, it enables risk control. In this study, we propose a novel approach called STRONG-Q that integrates efficient quantile-based factor screening methods into the framework of STRONG, which is a newly-developed Response-Surface-based framework, for large-scale quantile-based simulation optimization problems. The quantile-based factor screening method can effectively control the Type I error and enables the large-scale quantile-based simulation optimization problems to be solved efficiently when it is integrated into STRONG.
Chen, Yu-Ju, and 陳郁筑. "Re-appraisal of large-scale community-based hepatitis screenings in a township by two non-government organizations." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/fp5a2s.
Повний текст джерела高雄醫學大學
藥學系碩士在職專班
106
Background: Outcomes of Hepatitis B prevention and control in Taiwan is outstanding in the world. Hepatitis B, significant efforts have also been devoted to the prevention and control of Hepatitis C, including reimbursement of oral direct-acting antiviral drugs by the National Health Insurance. DAA can block disease progression for patients with chronic Hepatitis C infection. In Taiwan, screening by liver-related foundations has been widely known under the spread of newspaper media, and also believed by some to be a good screening model. In this study, we re-appraised this screening model from the epidemiological and clinical perspectives. Aims: To retrospectively analyze community-based screening data and discuss the effectiveness and unresolved issues of large-scale community screening from clinical perspectives. Methods: Two non-governmental organizations conducted a two-stage liver cancer screening in Daliao District, Kaohsiung City. The first stage was blood sampling to test HBsAg anti-HCV, AST , ALT and AFP. For those who were positive for HBsAg or anti-HCV, further confirmation were performed on HBV DNA and HCV RNA. The second stage was ultrasonography examination for participants who were positive for any of the above five markers. Results: A total of 1495 subjects participate in this screening. The median coverage rates of 22 villages were 7.93/1000. Overall prevalence of HBsAg and anti-HCV were 11.6% and 3.7%. To identify the candidates of antiviral treatment for chronic Hepatitis B and C virus infection, participants were stratified by viral load and ALT levels. Among the positive subjects, only less than half were positive for HBV DNA (5.3%) or HCV RNA (1.5%). To identify the high risk for HCC by REACH-B risk score, only 16 (9.2%) of HBsAg carrier were with score higher than 10. Five subjects had AFP levels higher than 20 ng/ml. Discussion: In the study area with same prevalence of HBsAg and anti-HCV as the whole of Taiwan, we found that only few candidates for anti-viral treatment or HCC screening can be detected through such a community-based screening. We noted that geographic representation of participants to the whole population was poor. Conclusion: Due to biased geographic sampling and low rate of candidates for intervention, community-based screening should be conducted with well-coverage design in high risk areas.