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Статті в журналах з теми "Juice expression"

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Simonyan, Kayode, Onu Onu, Chukwuma Kadurumba, Osemedua Okafor-Yadi, and Tosin Paul. "Design and development of a multi-tasks motorized ginger rhizomes juice expression machine." Poljoprivredna tehnika 47, no. 3 (2022): 56–66. http://dx.doi.org/10.5937/poljteh2203056s.

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Manual ginger juice expression processes are tedious, unhygienic, and inefficient, affecting the quality and quantity of ginger juice extracted. Extraction by chemical means is complex, expensive, and requires high skill to operate. The developed motorized ginger juice expression machine performs two distinct unit operations: size reduction and separation processes. Major components of the machine include the feeding unit, pulverizing unit, juice expression unit, juice drainage point, waste outlet, frame, and power transmission system. The machine is powered by 2 H.P., 1400 rpm and 1 H.P., 1430 rpm prime movers, and the V-belts and pulley assembly speed are 646 rpm and 240 rpm, respectively. The developed expression machine offers an affordable and simple method of processing fresh ginger minimizing loss in ginger rhizome quantity and quality, and ultimately reducing postharvest losses.
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Brovelli, Ernesto A., Alain Y. Boucher, John T. Arnason, Gopi R. Menon, and Rodney M. Johnson. "Effects of Expression Technology on Physical Parameters and Phytochemical Profile of Echinacea purpurea Juice." HortScience 39, no. 1 (February 2004): 146–48. http://dx.doi.org/10.21273/hortsci.39.1.146.

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The objective of this study was to compare the quality of E. purpurea juice obtained using two types of press, a hydraulic bag press (BP) and a mechanical screw press (SP). Cichoric acid concentration was about twice as high in the SP juice compared to the BP juice. Qualitative and quantitative differences in the alkylamides were observed, in favor of the SP. BP juice had a significantly higher pH, density and viscosity. No differences were observed in levels of soluble or total solids. The results of this study suggest that SP is more suitable for Echinacea juice expression than BP. Engineering and juice quality considerations in the selection of expression technology for medicinal plants are discussed in the context of therapeutic value.
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Peng, Wei, Jianhong Liu, Xiaorong Zhou, Hong Fan, Jianwei Lu, Senqing Chen, and Jifeng Feng. "Gastric juice piR-1245: A promising prognostic biomarker for gastric cancer." Journal of Clinical Oncology 38, no. 15_suppl (May 20, 2020): e16574-e16574. http://dx.doi.org/10.1200/jco.2020.38.15_suppl.e16574.

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e16574 Background: Emerging reports demonstrated that PIWI-interacting RNAs (piRNAs) played an indispensable role in tumorigenesis. However, it still remains elusive whether piR-1245 in gastric juice specific in stomach could be employed as a biomarker for gastric cancer (GC). The present work is aiming at exploring the possibility of piR-1245 in gastric juice as a potential marker to judge for diagnosis and prognosis of gastric cancer. Methods: Gastric juice was collected from 66 GC patients and 66 healthy individuals. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was employed to measure the levels of piR-1245 expression. Then, the pattern of piR-1245 expression in gastric juice was determined between GC patients and healthy individuals. A receiver operating characteristic (ROC) curve was constructed for distinguishing GC from healthy individuals. Results: Gastric juice piR-1245 levels in GC were higher than those of controls (P < 0.0001). The value of area under ROC (AUC) was 0.885 (sensitivity, 90.9%; specificity, 74.2%; 95% confidence interval, 0.8286 to 0.9414). High gastric juice piR-1245 expression was signally correlated with tumor size (P = 0.013) and TNM stage (P = 0.001). GC patients with high piR-1245 expression in gastric juice exerted a poorer overall survival (OS) (P = 0.0152) and progression-free survival (PFS) (P = 0.013). COX regression analysis verified that gastric juice piR-1245 expression was an independent prognostic risk variable for OS (P < 0.05). Conclusions: The current study suggested that piR-1245 in gastric juice had the potential to be a useful biomarker for GC detection and prognosis prediction.
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Bergholz, Teresa M., Sivapriya Kailasan Vanaja, and Thomas S. Whittam. "Gene Expression Induced in Escherichia coli O157:H7 upon Exposure to Model Apple Juice." Applied and Environmental Microbiology 75, no. 11 (April 3, 2009): 3542–53. http://dx.doi.org/10.1128/aem.02841-08.

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ABSTRACT Escherichia coli O157:H7 has caused serious outbreaks of food-borne illness via transmission in a variety of food vehicles, including unpasteurized apple juice, dried salami, and spinach. To understand how this pathogen responds to the multiple stresses of the food environment, we compared global transcription patterns before and after exposure to model apple juice. Transcriptomes of mid-exponential- and stationary-phase cells were evaluated after 10 min in model apple juice (pH 3.5) using microarrays probing 4,886 open reading frames. A total of 331 genes were significantly induced upon exposure of cells to model apple juice, including genes involved in the acid, osmotic, and oxidative stress responses as well as the envelope stress response. Acid and osmotic stress response genes, including asr, osmC, osmB, and osmY, were significantly induced in response to model apple juice. Multiple envelope stress responses were activated as evidenced by increased expression of CpxR and Rcs phosphorelay-controlled genes. Genes controlled by CpxR (cpxP, degP, and htpX) were significantly induced 2- to 15-fold upon exposure to apple juice. Inactivation of CpxRA resulted in a significant decrease in survival of O157:H7 in model apple juice compared to the isogenic parent strain. Of the 331 genes induced in model apple juice, 104 are O157-specific genes, including those encoding type three secretion effectors (espJ, espB, espM2, espL3, and espZ). Elucidating the response of O157:H7 to acidic foods provides insight into how this pathogen is able to survive in food matrices and how exposure to foods influences subsequent transmission and virulence.
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Rayasilp, Kritsakorn, Piyanuch Wonganan, Pajaree Chariyavilaskul, Nuntaporn Prompila, Varumporn Sukkummee, and Supeecha Wittayalertpanya. "Effect of Pomelo Juice on the Pharmacokinetics of Simvastatin, CYP3A2 Activity and Mdr1a, Mdr1b and Slc21a5 Expressions in Rats." Pharmaceutical Sciences 25, no. 4 (December 20, 2019): 303–10. http://dx.doi.org/10.15171/ps.2019.45.

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Background: Food-drug interaction can decrease drug effectiveness or increase risk of drug toxicity. Simvastatin is widely used for treatment of hypercholesterolemia and hypertriglyceridemia. Therefore, this study aimed to investigate the effects of pomelo juice on the pharmacokinetics of simvastatin, CYP3a2 activity and Mdr1a, Mdr1b and Slc21a5 expressions in rats. Methods: Rats were divided into 4 groups including (i) control, (ii) pomelo that received pomelo juice orally twice daily for 7 days, (iii) simvastatin that received simvastatin on day 8, and (iv) simvastatin + pomelo juice. Plasma concentrations of simvastatin and simvastatin acid were analyzed using LC-MS/MS. Hepatic CYP3a2 activity was evaluated using midazolam hydroxylation assay. The expressions of hepatic and intestinal Mdr1a, Mdr1b and Slc21a5 were measured using the real-time RT-PCR. Results: Oral administration of pomelo juice for 7 days altered pharmacokinetic profiles of simvastatin and its primary active metabolite, simvastatin acid, in rats. Real-time RT-PCR analysis revealed that pomelo juice significantly suppressed the expression of intestinal Mdr1a and Mdr1b and hepatic Slc21a5. Rat hepatic CYP3a2 catalytic activity was also inhibited following pomelo juice administration. Conclusion: The results of this study suggested that there was a risk of potential drug interaction associated with inhibition of drug transporters and CYP3A caused by pomelo juice.
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Gualtieri, Paola, Marco Marchetti, Giulia Frank, Antonella Smeriglio, Domenico Trombetta, Carmela Colica, Rossella Cianci, Antonino De Lorenzo, and Laura Di Renzo. "Antioxidant-Enriched Diet on Oxidative Stress and Inflammation Gene Expression: A Randomized Controlled Trial." Genes 14, no. 1 (January 13, 2023): 206. http://dx.doi.org/10.3390/genes14010206.

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The Mediterranean Diet (MedDiet) is associated with beneficial effects against chronic non-communicable diseases (CNCDs). In particular, the content of micronutrients leads to an improvement of the oxidative and inflammatory profiles. A randomized, parallel, controlled study, on 24 subjects, was conducted to evaluate if 2-week supplementation with a mixed apple and bergamot juice (MAB juice), had a positive impact on the body composition, the biochemical profile, and oxidative and inflammatory gene expression (Superoxide dismutase (SOD1), Peroxisome Proliferator-Activated Receptor γ (PPARγ), catalase (CAT), chemokine C-C motif ligand 5 (CCL5), Nuclear Factor Kappa B Subunit 1 (NFKB1), Vitamin D Receptor (VDR), and Macrophage Migration Inhibitory Factor (MIF)), respect to a MedDiet. Body composition evaluation analysis showed a gain in lean mass (p < 0.01). Moreover, a significant reduction in total cholesterol/HDL index (p < 0.01) was pointed out between the two groups. Gene expression analysis highlighted an increase in MIF (p ≤ 0.05), PPARγ (p < 0.001), SOD1 (p ≤ 0.05), and VDR (p ≤ 0.05) expressions when comparing MedDiet and MedDiet + MAB juice groups. These data based on the nutrigenomics approach demonstrated that supplementing 2 weeks of MAB juice to the MedDiet could contribute to a reduction in the risk of CNCDs.
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Elvira-Torales, Laura, Inmaculada Navarro-González, Rocío González-Barrio, Gala Martín-Pozuelo, Guillermo Doménech, Juan Seva, Javier García-Alonso, and María Periago-Castón. "Tomato Juice Supplementation Influences the Gene Expression Related to Steatosis in Rats." Nutrients 10, no. 9 (September 2, 2018): 1215. http://dx.doi.org/10.3390/nu10091215.

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The objective of this work was to identify the effect of tomato juice on the expression of genes and levels of metabolites related to steatosis in rats. Male Sprague Dawley rats (8 weeks-old) were grouped (6 rats/group) in four experimental groups: NA (normal diet and water), NL (normal diet and tomato juice), HA (high-fat diet and water), and HL (high-fat diet and tomato juice). After an intervention period of 5 weeks, rats were sacrificed and biochemical parameters, biomarkers of oxidative stress, liver metabolites, and gene expression were determined. Although the H diet provoked dislipemia related to steatosis, no changes in isoprostanes or liver malondialdehyde (MDA) were observed. Changes in the gene expression of the HA group were produced by the high consumption of fat, whereas the consumption of tomato juice had different effects, depending on the diet. In the NL group, the genes involved in β-oxidation were upregulated, and in groups NL and HL upregulation of CD36 and downregulation of APOB and LPL were observed. In addition, in the HL group the accumulation of lycopene upregulated the genes FXR and HNF4A, which have been suggested as preventive factors in relation to steatosis. Regarding the metabolomics study, intake of tomato juice stimulated the biosynthesis of glutathione and amino acids of the transulfurization pathway, increasing the levels of metabolites related to the antioxidant response.
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Li, Xiaoting, Hantang Huang, Hafiz Muhammad Rizwan, Naiyu Wang, Jingyi Jiang, Wenqin She, Guohua Zheng, et al. "Transcriptome Analysis Reveals Candidate Lignin-Related Genes and Transcription Factors during Fruit Development in Pomelo (Citrus maxima)." Genes 13, no. 5 (May 9, 2022): 845. http://dx.doi.org/10.3390/genes13050845.

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Juice sac granulation (a physiological disorder) leads to large postharvest losses of pomelo (Citrus maxima). Previous studies have shown that juice sac granulation is closely related to lignin accumulation, while the molecular mechanisms underlying this disorder remain elusive in pomelo. Our results showed that the lignin content in NC (near the core) and FC (far away from the core) juice sacs overall increased from 157 DPA (days post anthesis) to 212 DPA and reached a maximum at 212 DPA. Additionally, the lignin content of NC juice sacs was higher than that of FC juice sacs. In this study, we used transcriptome-based weighted gene co-expression network analysis (WGCNA) to address how lignin formation in NC and FC juice sacs is generated during the development of pomelo. After data assembly and bioinformatic analysis, we found a most correlated module (black module) to the lignin content, then we used the 11 DEGs in this module as hub genes for lignin biosynthesis. Among these DEGs, PAL (phenylalanine ammonia lyase), HCT (hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase), 4CL2 (4-coumarate: CoA ligase), C4H (cinnamate 4-hydroxylase), C3′H (p-coumarate 3-hydroxylase), and CCoAOMT1 (caffeoyl CoA 3-Omethyltransferase) were the most distinct DEGs in granulated juice sacs. Co-expression analysis revealed that the expression patterns of several transcription factors such as MYB, NAC, OFP6, and bHLH130 are highly correlated with lignin formation. In addition, the expression patterns of the DEGs related to lignin biosynthesis and transcription factors were validated by qRT-PCR, and the results were highly concordant with the RNA-seq results. These results would be beneficial for further studies on the molecular mechanism of lignin accumulation in pomelo juice sacs and would help with citrus breeding.
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Dávalos, Alberto, Gema de la Peña, Carolina C. Sánchez-Martín, M. Teresa Guerra, Begoña Bartolomé, and Miguel A. Lasunción. "Effects of red grape juice polyphenols in NADPH oxidase subunit expression in human neutrophils and mononuclear blood cells." British Journal of Nutrition 102, no. 8 (May 19, 2009): 1125–35. http://dx.doi.org/10.1017/s0007114509382148.

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The NADPH oxidase enzyme system is the main source of superoxide anions in phagocytic and vascular cells. NADPH oxidase-dependent superoxide generation has been found to be abnormally enhanced in several chronic diseases. Evidence is accumulating that polyphenols may have the potential to improve cardiovascular health, although the mechanism is not fully established. Consumption of concentrated red grape juice, rich in polyphenols, has been recently shown to reduce NADPH oxidase activity in circulating neutrophils from human subjects. In the present work we studied whether red grape juice polyphenols affected NADPH oxidase subunit expression at the transcription level. For this, we used human neutrophils and mononuclear cells from peripheral blood, HL-60-derived neutrophils and the endothelial cell line EA.hy926.Superoxide production was measured with 2′7′-dichlorofluorescein diacetate or lucigenin, mRNA expression by real-time RT-PCR and protein expression by Western blot. Each experiment was performed at least three times. In all cell types tested, red grape juice, dealcoholised red wine and pure polyphenols decreased superoxide anion production. Red grape juice and dealcoholised red wine selectively reduced p47phox, p22phox and gp91phox expression at both mRNA and protein levels, without affecting the expression of p67phox. Pure polyphenols, particularly quercetin, also reduced NADPH oxidase subunit expression, especially p47phox, in all cell types tested. The present results showing that red grape juice polyphenols reduce superoxide anion production provide an alternative mechanism by which consumption of grape derivatives may account for a reduction of oxidative stress associated with cardiovascular and/or inflammatory diseases related to NADPH oxidase superoxide overproduction.
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Saimin, Juminten, Hendy Hendarto, and Soetjipto Soetjipto. "The Effect of Tomato Juice in Increasing Ki-67 Expression and Epithelial Thickness on The Vaginal Wall of Menopausal Rats." Indonesian Biomedical Journal 11, no. 2 (August 1, 2019): 152–8. http://dx.doi.org/10.18585/inabj.v11i2.517.

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BACKGROUND: Dyspareunia and pain due to the decrease of vaginal wall thickness usually happen in menopausal women. The reduction of estrogen levels cause the decreasing of cell proliferation and the thinning of vaginal wall epithelium. Tomato (Solanum lycopersicum) is a source of phytoestrogens, which produce estrogenic effects. This study aims to assess the effect of tomato juice on Ki-67 expression and epithelial thickness of the vaginal wall in menopausal rats.METHODS: This was an experimental study using Sprague-Dawley rats. Twenty-four female rats, aged 4 months and weighing 150-200 grams, were divided into 4 groups. Each group consisted of 6 rats. Negative control (NC) group was group of rats with sham procedure and performed by distilled water for 28 days. Positive control (PC) group was group of bilateral ovariectomized rats and performed by distilled water for 28 days. The first treatment (T1) group was group of bilateral ovariectomized rats, given tomato juice at dose of 11g/200g body weight (BW)/day. The second treatment (T2) group was group of bilateral ovariectomized rats, given tomato juice at dose of 15g/200g BW/day. Data analysis was done with Anova, multiple comparisons and regression test.RESULTS: The group with lowest Ki-67 expression was PC group (2.52±0.60). The expression of Ki-67 in treatment groups (T1 and T2) was higher than PC group, but lower than NC group. There was no significant difference between groups (p=0.771). The lowest epithelial thickness was found in PC group (21.19±3.96) and the highest was found in the treatment groups (38.73±12.43). There was positive correlation between tomato juice and epithelial thickness (p=0.647).CONCLUSION: Tomato juice increases Ki-67 expression and epithelial thickness on the vaginal wall of menopausal rats. The increase of epithelial thickness follows the administration dose, but Ki-67 expression does not exceed in the control rats. Tomato juice increases the cell proliferation of vaginal wall on menopausal rats, however the increase is still within normal limits.KEYWORDS: epithelial thickness, Ki-67, menopause, tomato juice
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Дисертації з теми "Juice expression"

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GUICHARD, TAYAR MARGUERITE. "Le systeme cantilatoire de la bible et son expression musicale dans les traditions juives des communautes mediterraneennes." Paris 8, 1987. http://www.theses.fr/1987PA080136.

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Apres notre etude sur la musique dans la communaute israelite de marseille des origines a nos jours, nous avons entrepris d'etendre notre recherche a tout le bassin mediterraneen et donc de nous attacher a retrouver et retranscrire toute la musique juive mediterraneenne. Notre travail a donc ete d'abord un travail d'enquete musicologique, ou si l'on veut, une quete de la tradition musicale juive la plus authentique de cette region. Ensuite, et cela represente le corps principal de l'etude, nous avons procure une edition de ces diverses traditions musicales en un recueil de quarante deux morceaux musicaux liturgiques. Cette musique liturgique presente une particularite- c'est de la cantilation c'est a dire que c'est une forme d'intonation des textes bibliques a la limite entre la parole ( la recitation ) et la musique (le chant). Il fallait donc essayer de retrouver sa parente, ou, par defaut, de faire ressortir ses ressemblances avec ces deux formes d'expression. En particulier et c'est la un caractere tout a fait original de cette musique, on peut reperer dans le texte biblique des signes, les te'amim qui ont une signification ( et donc sont interpretes ) dans l'execution musica- le. Quels etaient donc ces signes? des signes de ponctuation? des orne- ments (comme dans la musique du dix-huitieme siecle?, l'un et l'autre, ou ni l'un ni l'autre, puisque ce sont des signes intermediaires entre parole et musique. Il fallait donc faire la part des choses et analy- ser tres precisement en quoi les te'amim transcrivaient des courbes prosodiques et en quoi ils correspondaient a des courbes musicales, apres les avoir au prealable inventories et decrits. Au cours de cette recherche nous avons ete amenee a mettre au point, des methodes d'a- nalyse de la melodie, forgees par analogie sur celles de l'acousti- que musicale ou presentant une application directe des methodes d'ana- lyse mathematique. Puisque cette tradition doit vivre, se perpetuer, nous avons propose une methode fondee sur notre analyse et nos experiences
This study is essentially an edition's work. We wanted to collect all the musical traditions among the hebraic communities of the mediterranean countries. We have recorded many rabbies of severaltraditions and then transcribed their musical execution in a common written musical notation. The music we discovered was very surprising, original, and we tried to know more about it. The melody was a kind of inflection's form between speech and music. Was it speech? was it music? we weretempted to say both and we tried to show in which way. Especially, thereis, in the biblical text, particular punctuation signs called'ta'amim'. But these signs were not only punctuation signs because they were executed in a musical form, and we tried to show that they were at thesame time prosodic features-corresponding to particular intonation curves-and what we could call"musical features", just likes the musical ornaments, corresponding to particular melodic curves. For the analysis, we had to find new methods, especially for the melody, which was analysed in pitch variations (pitch raising or lowering) and rhythm variations with a method issued from acoustic analysis. We tried also to apply to melody a logical theory, thefuzzy sets theory. And, in the last section, we wanted to show that this analysis could be applied to a pedagogical purpose and to explain how, with a proper analysis, the ta'am could be taught to children
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Nordestgaard, Simon Jonathan. "Phenolic extraction and juice expression during white wine production." Thesis, 2011. http://hdl.handle.net/2440/70292.

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Phenolic compounds are important constituents of white wine that can impart bitterness and astringency and influence stability and colour. The specific types and concentrations of phenolic compounds vary between the different grape tissues. For example grape skins contain much higher concentrations of phenolics than grape pulp. Consequently, grape processing techniques, particularly the method by which juice is expressed, can influence the phenolic profile of white wines. Concerns of elevated phenolic levels tend to lead to conservative practices such as limiting the time between machine harvesting and winery processing, and the use of relatively expensive batch draining and pressing equipment. Phenolic extraction during pomace contact, such as might occur after machine harvesting prior to winery processing, was investigated at a controlled laboratory scale. Contact time significantly increased phenolic extraction as did the fraction of broken berries. However, given the uncertainty in the amount of berry breakage in industrial practice, full scale transport trials should be performed by wineries where economic advantage could be gained from relaxing restriction on times between harvesting and winery processing. Techniques used for white grape juice expression both now and in the past were critically reviewed and winery sampling was performed to develop a practical understanding of operational issues. For the expression of juice from white grapes, pneumatic membrane presses have been increasingly adopted both for pressing and draining. This is principally a product of their ability to express high yields of juice with relatively low levels of phenolics and suspended solids. These devices can be operated in many different manners and quality and productivity is highly dependent on the specific mode of operation. Adaptive programmes based principally on continuous assessment of juice flow rate, and the use of conductivity measurement to monitor skin extraction are important tools that can aid economic optimisation of expression operations. The principal problem with the pneumatic membrane press is that it is a batch operation with a relatively low throughput. In the longer term, the ideal outcome would be the development of expression equipment that achieved the high throughput and relatively low cost per tonne of continuous inclined drainers and screw presses, while still maintaining the quality obtained with pneumatic membrane presses. Continuous devices that mimic the repeated cycles of compression and crumbling of these batch presses may be one means of achieving this. Exploration of the different continuous screw presses used historically suggests that there still may be room for improvement in these devices. This merits further specific investigation. Experiments involving repeated cycles of compression and crumbling were performed with a constant rate laboratory apparatus. These experiments demonstrated the importance of bed height, pressing speed, sieve plate design and crumbling on press operation. Solids content appears to be a major issue in the development of rapid juice expression equipment. One means of achieving appropriately low solids levels, without requiring large devices with large cake beds for juice filtration, may be to maintain the structure of the grape for as long as possible so that juice is filtered as it is expelled from the berry.
Thesis (Ph.D.) -- University of Adelaide, School of Chemical Engineering, 2011
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Частини книг з теми "Juice expression"

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Wang, Jin Zhi, Gad G. Yousef, Randy B. Rogers, Elvira Gonzalez de Mejia, Ilya Raskin, and Mary Ann Lila. "Maqui Berry (Aristotelia chilensis) Juices Fermented with Yeasts: Effects on Phenolic Composition, Antioxidant Capacity, and iNOS and COX-2 Protein Expression." In ACS Symposium Series, 95–116. Washington, DC: American Chemical Society, 2012. http://dx.doi.org/10.1021/bk-2012-1093.ch006.

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Grunert, Klaus, Jacob Rosendahl, Andreas I. Andronikidis, George J. Avlonitis, Paulina Papastathopoulou, Carmen R. Santos, Ana R. Pertejo, et al. "Drinking Motives." In Consumption Culture in Europe, 306–32. IGI Global, 2013. http://dx.doi.org/10.4018/978-1-4666-2857-1.ch008.

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This chapter presents an analysis of what consumer in Europe drink and why they drink what they drink. The concept of drinking motives is developed and defined, and analysis of data on drinking motives shows that these can be grouped into two major classes: self-expressive and functional. This distinction is universal and hence applies across Europe. However, the importance of self-expressive as compared to functional motives, as well as the way in which these relate to different beverages, does differ across Europe. Both dimensions are relevant for the motives for drinking non-alcoholic drinks, whereas the self-expression dimension dominates reasons for drinking alcoholic drinks. The Eastern European countries have generally higher scores on the self-expression dimension, indicating that such motives play a bigger role there compared to the other regions. No clear geographical pattern emerged with regard to the functional dimension. Beer and spirits are the alcoholic drinks and tea, water, and juice the non-alcoholic drinks that are related to high scores on the self-expression dimension. Water and juice are the drinks scoring highest on functionality, being mainly related to health, availability, and quenching one’s thirst. The non-alcoholic products scoring low on functionality are coffee, tea, soft drinks, and energy drinks. Analysis of socio-demographic differences resulted in only a few effects. Men, lower education groups, and lower income groups are more likely to drink alcohol for reasons other than self-expression motives (such as to quench one’s thirst). Also, the health motive plays a larger role for older people, and the self-expressive and social motives play a larger role for younger people. The chapter closes with recommendations for the marketing of drink products in Europe.
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Тези доповідей конференцій з теми "Juice expression"

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Janzowski, Christine, Bulent Soyalan, Franz Albert, Thomas Rath, Helmut Dietrich, Frank Will, and Gerhard Eisenbrand. "Abstract B61: Modulation of antioxidant gene expression by a fruit juice rich in polyphenols: an intervention study with hemodialysis patients." In Abstracts: Frontiers in Cancer Prevention Research 2008. American Association for Cancer Research, 2008. http://dx.doi.org/10.1158/1940-6207.prev-08-b61.

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Kalamaki, Mary S., David A. Brummell, Mark H. Harpster, Joseph M. Palys, John M. Labavitch, Neal Gutterson, and David S. Reid. "SUPPRESSION OF RIPENING-RELATED POLYGALACTURONASE AND EXPANSIN GENE EXPRESSION AFFECTS THE PHYSICAL AND BIOCHEMICAL PROPERTIES OF JUICE CONCENTRATES AND RESULTS IN IMPROVED VISCOSITY CHARACTERISTICS." In XXIst International Carbohydrate Symposium 2002. TheScientificWorld Ltd, 2002. http://dx.doi.org/10.1100/tsw.2002.784.

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Yigit, Funda. "Effects of morinda citrifolia (noni) juice on apoptosis and expression of neutrophil gelatinase-associated lipocalin in the kidney of rats exposed to 3-methyl-4-nitrophenol." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.pp-25.

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Yonezawa, Suguru, Seiya Yokoyama, and Michiyo Higashi. "Abstract 1897: Application of a novel DNA methylation analysis method (MSE) for mucin expression in pancreatic juices for diagnosis of pancreatic neoplasms." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-1897.

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Звіти організацій з теми "Juice expression"

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Blumwald, Eduardo, and Avi Sadka. Citric acid metabolism and mobilization in citrus fruit. United States Department of Agriculture, October 2007. http://dx.doi.org/10.32747/2007.7587732.bard.

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Анотація:
Accumulation of citric acid is a major determinant of maturity and fruit quality in citrus. Many citrus varieties accumulate citric acid in concentrations that exceed market desires, reducing grower income and consumer satisfaction. Citrate is accumulated in the vacuole of the juice sac cell, a process that requires both metabolic changes and transport across cellular membranes, in particular, the mitochondrial and the vacuolar (tonoplast) membranes. Although the accumulation of citrate in the vacuoles of juice cells has been clearly demonstrated, the mechanisms for vacuolar citrate homeostasis and the components controlling citrate metabolism and transport are still unknown. Previous results in the PIs’ laboratories have indicated that the expression of a large number of a large number of proteins is enhanced during fruit development, and that the regulation of sugar and acid content in fruits is correlated with the differential expression of a large number of proteins that could play significant roles in fruit acid accumulation and/or regulation of acid content. The objectives of this proposal are: i) the characterization of transporters that mediate the transport of citrate and determine their role in uptake/retrieval in juice sac cells; ii) the study of citric acid metabolism, in particular the effect of arsenical compounds affecting citric acid levels and mobilization; and iii) the development of a citrus fruit proteomics platform to identify and characterize key processes associated with fruit development in general and sugar and acid accumulation in particular. The understanding of the cellular processes that determine the citrate content in citrus fruits will contribute to the development of tools aimed at the enhancement of citrus fruit quality. Our efforts resulted in the identification, cloning and characterization of CsCit1 (Citrus sinensis citrate transporter 1) from Navel oranges (Citrus sinesins cv Washington). Higher levels of CsCit1 transcripts were detected at later stages of fruit development that coincided with the decrease in the juice cell citrate concentrations (Shimada et al., 2006). Our functional analysis revealed that CsCit1 mediates the vacuolar efflux of citrate and that the CsCit1 operates as an electroneutral 1CitrateH2-/2H+ symporter. Our results supported the notion that it is the low permeable citrateH2 - the anion that establishes the buffer capacity of the fruit and determines its overall acidity. On the other hand, it is the more permeable form, CitrateH2-, which is being exported into the cytosol during maturation and controls the citrate catabolism in the juice cells. Our Mass-Spectrometry-based proteomics efforts (using MALDI-TOF-TOF and LC2- MS-MS) identified a large number of fruit juice sac cell proteins and established comparisons of protein synthesis patterns during fruit development. So far, we have identified over 1,500 fruit specific proteins that play roles in sugar metabolism, citric acid cycle, signaling, transport, processing, etc., and organized these proteins into 84 known biosynthetic pathways (Katz et al. 2007). This data is now being integrated in a public database and will serve as a valuable tool for the scientific community in general and fruit scientists in particular. Using molecular, biochemical and physiological approaches we have identified factors affecting the activity of aconitase, which catalyze the first step of citrate catabolism (Shlizerman et al., 2007). Iron limitation specifically reduced the activity of the cytosolic, but not the mitochondrial, aconitase, increasing the acid level in the fruit. Citramalate (a natural compound in the juice) also inhibits the activity of aconitase, and it plays a major role in acid accumulation during the first half of fruit development. On the other hand, arsenite induced increased levels of aconitase, decreasing fruit acidity. We have initiated studies aimed at the identification of the citramalate biosynthetic pathway and the role(s) of isopropylmalate synthase in this pathway. These studies, especially those involved aconitase inhibition by citramalate, are aimed at the development of tools to control fruit acidity, particularly in those cases where acid level declines below the desired threshold. Our work has significant implications both scientifically and practically and is directly aimed at the improvement of fruit quality through the improvement of existing pre- and post-harvest fruit treatments.
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Sadka, Avi, Mikeal L. Roose, and Yair Erner. Molecular Genetic Analysis of Citric Acid Accumulation in Citrus Fruit. United States Department of Agriculture, March 2001. http://dx.doi.org/10.32747/2001.7573071.bard.

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Анотація:
The acid content of the juice sac cells is a major determinant of maturity and fruit quality in citrus. Many citrus varieties accumulate acid in concentrations that exceed market desires, reducing grower income and consumer satisfaction. Pulp acidity is thought to be dependent on two mechanisms: the accumulation of citric acid in the vacuoles of the juice sac cells, and acidification of the vacuole. The major aim of the project was to direct effort toward understanding the mechanism of citric acid accumulation in the fruit. The following objectives were suggested: Measure the activity of enzymes likely to be involved in acid accumulation and follow their pattern of expression in developing fruit (Sadka, Erner). Identify and clone genes which are associated with high and low acid phenotypes and with elevated acid level (Roose, Sadka, Erner). Convert RAPD markers that map near a gene that causes low acid phenotype to specific co dominant markers (Roose). Use genetic co segregation to test whether specific gene products are responsible for low acid phenotype (Roose and Sadka). Objective 1 was fully achieved. Most of the enzymes of organic acid metabolism were cloned from lemon pulp. Their expression was studied during fruit development in low and high acid varieties. The activity and expression of citrate synthase, aconitase and NADP-isocitrate dehydrogenase (IDH) were studied in detail. The role that each enzyme plays in acid accumulation and decline was evaluated. As a result, a better understanding of the metabolic changes that contribute to acid accumulation was achieved. It was found that the activity of the mitochondrial aconitase is greatly reduced early in high-acid fruits, but not in acidless ones, suggesting that this enzyme plays an important role in acid accumulation. In addition, it was demonstrated that increases in the cytosolic forms of aconitase and NADP-IDH towards fruit maturation play probably a major role in acid decline. Our studies also demonstrated that the two mechanisms that contribute to fruit acidity, vacuolar acidification and citric acid accumulation, are independent, although they are tightly co-regulated. Additional, we demonstrated that sodium arsenite, which reduce fruit acidity, causes a transient inhibition in the activity of citrate synthase, but an induction in the gene expression. This part of the work has resulted in 4 papers. Objective 3 was also fully achieved. Using bulked segregant analysis, three random amplified polymorphic DNA (RAPD) markers were identified as linked to acitric, a gene controlling the acidless phenotype of pummelo 2240. One of them, which mapped 1.2 cM from acitric was converted into sequence characterized amplified region (SCAR marker, and into co dominant restriction length polymorphism (RFLP) marker. These markers were highly polymorphic among 59 citrus accessions, and therefore, they should be useful for selecting seedling progeny heterozygous for acitric in nearly all crosses between pummelo 2240 and other citrus genotypes. This part of the project resulted in one paper. Objective 4 was also fully achieved. Clones isolated by the Israeli group were sent to the American laboratory for co segregation analysis. However, none of them seemed to co segregate with the low acid phenotype. Both laboratories invested much effort in achieving the goals of Objective 2, namely the isolation of genes that are elevated in expression in low and high acid phenotypes, and in tissue cultures treated with arsenite (a treatment which reduces fruit acidity). However, conventional differential display and restriction fragment differential display analyses could not identify any differentially expressed genes. The isolation of such genes was the major aim of a continuation project, which was recently submitted.
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Porat, Ron, Doron Holland, and Linda Walling. Identification of Citrus Fruit-Specific and Pathogen-Induced Promoters and Their Use in Molecular Engineering. United States Department of Agriculture, January 2001. http://dx.doi.org/10.32747/2001.7585202.bard.

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This one year BARD project was funded to develop methods to monitor promoter activity a gene expression patterns in citrus fruit. To fulfill this goal, we divided the research tasks between both labs so that the Israeli side evaluated the use of microprojectile bombardment ; a tool to evaluate transient gene expression in various citrus fruit tissues, and the US side optimized technical parameters required for Agrobacterium-mediated transformation of various citrus cultivars. Microprojectile bombardment appeared to be a very efficient method for transient gene expression analysis in citrus leaf tissues but was somewhat less applicable in fruit tissues. Nevertheless, we did succeeded to achieve significant levels of 35S-GUS gene expression in young green flavedo tissue. However, only single random spots of 35S-GUS gene expression were detected mature flavedo and in juice sacs and albedo tissue. Overall, we assume that following some more technical improvements particle bombardment could provide a useful technique to rapidly analyze promoter activity at least in the flavedo tissue. For Agrobacterium-mediated transformation, we found that shoot cultures of 'Washington' navel oranges,'Fairchild' mandarins,'Eureca' lemons,'Troyer' citrange and various grapefruits provided a more reliable and consistent source of tissue for transformation than germinated seedlings. Moreover, various growth media's (McCown, Quoirin & Lepoivre, DCR) further improved shoot and root growth relative to MS mineral media, which is commonly used. Also pure white light (using bulbs which do not emit UV or blue light) improved shoot growth in various citrus varieties, and paromomycin appeared to be a more efficient antibiotic for the selection of transgenic plants than Kanamycin. Overall, these optimizations improve transformation efficacy and shoot growth and rooting capacity. In addition to the development of transformation methods, both Israeli and US labs achieved progress in the identification of citrus fruit-specific promoters. In Israel, we isolated a 3.6 kb promoter fragment of the thiamine biosynthesis c-thi gene, which is highly expressed in fruit peel tissue, whereas in the US we isolated a 1.5 kb promoter fragment of the citrus seed-specific cDNA CssH. The identification of more fruit-specific cDNAs and their corresponding promoter regions is currently in progress.
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Crisosto, Carlos, Susan Lurie, Haya Friedman, Ebenezer Ogundiwin, Cameron Peace, and George Manganaris. Biological Systems Approach to Developing Mealiness-free Peach and Nectarine Fruit. United States Department of Agriculture, 2007. http://dx.doi.org/10.32747/2007.7592650.bard.

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Peach and nectarine production worldwide is increasing; however consumption is flat or declining because of the inconsistent eating quality experienced by consumers. The main factor for this inconsistent quality is mealiness or woolliness, a form of chilling injury that develops following shipping periods in the global fruit market today. Our research groups have devised various postharvest methods to prolong storage life, including controlled atmosphere and delayed storage; however, these treatments only delay mealiness. Mealiness texture results from disruption of the normal ripening process involving disassembly of cell wall material, and creates a soft fruit texture that is dry and grainy instead of juicy and smooth. Solving this problem is a prerequisite for increasing the demand for fresh peach and nectarine. Two approaches were used to reveal genes and their associated biochemical processes that can confer resistance to mealiness or wooliness. At the Volcani Center, Israel, a nectarine cultivar and the peach cultivar (isogenetic materials) from which the nectarine cultivar spontaneously arose, and at the Kearney Agricultural Center of UC Davis, USA, a peach population that segregates for quantitative resistance to mealiness was used for dissecting the genetic components of mealiness development. During our project we have conducted research integrating the information from phenotypic, biochemical and gene expression studies, proposed possible candidate genes and SNPs-QTLs mapping that are involved in reducing peach mealiness susceptibility. Numerous genes related to ethylene biosynthesis and its signal transduction, cell wall structure and metabolism, stress response, different transcription factor families were detected as being differentially accumulated in the cold-treated samples of these sensitive and less sensitive genotypes. The ability to produce ethylene and keep active genes involved in ethylene signaling, GTP-binding protein, EIN-3 binding protein and an ethylene receptor and activation of ethyleneresponsive fruit ripening genes during cold storage provided greater resistance to CI. Interestingly, in the functional category of genes differentially expressed at harvest, less chilling sensitive cultivar had more genes in categories related to antioxidant and heat sock proteins/chaperones that may help fruit to adapt to low temperature stress. The specific objectives of the proposed research were to: characterize the phenotypes and cell wall components of the two resistant systems in response to mealiness- inducing conditions; identify commonalities and specific differences in cell wall proteins and the transcriptome that are associated with low mealiness incidence; integrate the information from phenotypic, biochemical, and gene expression studies to identify candidate genes that are involved in reducing mealiness susceptibility; locate these genes in the Prunus genome; and associate the genes with genomic regions conferring quantitative genetic variation for mealiness resistance. By doing this we will locate genetic markers for mealiness development, essential tools for selection of mealiness resistant peach lines with improved fruit storability and quality. In our research, QTLs have been located in our peach SNPs map, and proposed candidate genes obtained from the integrated result of phenotypic, biochemical and gene expression analysis are being identified in our QTLs as an approach searching for consistent assistant markers for peach breeding programs.
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