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Статті в журналах з теми "IspG"

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Автор: Grafiati

Опубліковано: 7 липня 2024

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1

Guerra, Francisco, Ke Wang, Jikun Li, Weixue Wang, Yi-Liang Liu, Shivani Amin, and Eric Oldfield. "Inhibition of the 4Fe–4S proteins IspG and IspH: an EPR, ENDOR and HYSCORE investigation." Chem. Sci. 5, no. 4 (2014): 1642–49. http://dx.doi.org/10.1039/c3sc53301h.

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2

Zhou, Jia, Liyang Yang, Chonglong Wang, Eui-Sung Choi, and Seon-Won Kim. "Enhanced performance of the methylerythritol phosphate pathway by manipulation of redox reactions relevant to IspC, IspG, and IspH." Journal of Biotechnology 248 (April 2017): 1–8. http://dx.doi.org/10.1016/j.jbiotec.2017.03.005.

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3

Liu, Chunyu, Sian Hemmings, and Thomas G. Schulze. "ISPG GLOBAL DIVERSITY PROPOSAL." European Neuropsychopharmacology 29 (2019): S1039. http://dx.doi.org/10.1016/j.euroneuro.2018.07.039.

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4

Lu, Zhifang, Biying Wang, Zhiyu Qiu, Ruiling Zhang, Jimin Zheng, and Zongchao Jia. "YdfD, a Lysis Protein of the Qin Prophage, Is a Specific Inhibitor of the IspG-Catalyzed Step in the MEP Pathway of Escherichia coli." International Journal of Molecular Sciences 23, no. 3 (January 29, 2022): 1560. http://dx.doi.org/10.3390/ijms23031560.

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Bacterial cryptic prophage (defective prophage) genes are known to drastically influence host physiology, such as causing cell growth arrest or lysis, upon expression. Many phages encode lytic proteins to destroy the cell envelope. As natural antibiotics, only a few lysis target proteins were identified. ydfD is a lytic gene from the Qin cryptic prophage that encodes a 63-amino-acid protein, the ectopic expression of which in Escherichia coli can cause nearly complete cell lysis rapidly. The bacterial 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway is responsible for synthesizing the isoprenoids uniquely required for sustaining bacterial growth. In this study, we provide evidence that YdfD can interact with IspG, a key enzyme involved in the MEP pathway, both in vivo and in vitro. We show that intact YdfD is required for the interaction with IspG to perform its lysis function and that the mRNA levels of ydfD increase significantly under certain stress conditions. Crucially, the cell lysis induced by YdfD can be abolished by the overexpression of ispG or the complementation of the IspG enzyme catalysis product methylerythritol 2,4-cyclodiphosphate. We propose that YdfD from the Qin cryptic prophage inhibits IspG to block the MEP pathway, leading to a compromised cell membrane and cell wall biosynthesis and eventual cell lysis.

5

Trofimov, Leonid N., Ivan L. Trofimov, and Sergei V. Podkovalnikov. "North-east Asia interstate power grid expansion in the conditions of possible economic recession in China." E3S Web of Conferences 216 (2020): 01007. http://dx.doi.org/10.1051/e3sconf/202021601007.

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The article considers scenarios of the prospective interstate power grid (ISPG) expansion in Northeast Asia with possible economic recession in China because of COVID-19 and other factors. Features of optimization of new capacities addition of renewable energy sources in the ISPG are considered. A series of computational experiments with variations in the profiles of wind and solar power plants were carried out. The assessment of impact the intermittence of renewable energy power output on the structure of ISPG was evaluated, with taking into account of possible economic recession in China. A comparative analysis of solutions to the problem of optimization of intermittent generation capacity inputs for scenarios with the formation of an energy connection in northeast Asia and its absence is carried out.

6

Propping, Peter. "ISPG lifetime achievement award 2004." American Journal of Medical Genetics Part B: Neuropsychiatric Genetics 136B, no. 1 (2005): 1. http://dx.doi.org/10.1002/ajmg.b.30208.

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7

Lee, Mon-Chien, Yi-Ju Hsu, Li-Hsuan Yang, Chi-Chang Huang, and Chun-Sheng Ho. "Ergogenic Effects of Green Tea Combined with Isolated Soy Protein on Increasing Muscle Mass and Exercise Performance in Resistance-Trained Mice." Nutrients 13, no. 12 (December 18, 2021): 4547. http://dx.doi.org/10.3390/nu13124547.

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It is well known that supplementation with high protein after exercise can effectively promote muscle synthesis and repair, while green tea is rich in catechins that have antioxidant effects. We aimed to explore the effects of green tea combined with isolated soy protein on increase muscle mass in resistance-trained mice. A total of 32 male ICR mice (8-weeks old) were divided into four groups (n = 8/group), sedentary control group (SC), isolated soy protein with green tea group (ISPG), resistance training group (RT), isolated soy protein and green tea combine with resistance training group (ISPG + RT). All mice received control or ISPG by oral gavage for four consecutive weeks. Forelimb grip and exhaustive swimming time were used for exercise performance evaluation. In biochemical profile, we analyzed lactate, ammonia, blood urea nitrogen (BUN), and glucose and muscle damage index creatine kinase (CK) after exercise as biochemical parameters of exercise fatigue. The grip strength, muscular endurance, and exhaustive swimming time of the ISPG + RT group were significantly increased than other groups (p < 0.05), and also significantly decreased in serum lactate and ammonia levels (p < 0.05, respectively). The ISP + RT group was not only increased in quadriceps weight, (p < 0.05) but also decreased EFP (p < 0.05). We recommend using a 4-week supplementation with ISPG, combined with RT, to increase muscle mass, exercise performance, glycogen storage, and reduce fatigue biochemical parameters after exercise. The benefits of long-term supplementation or application to human supplementation can be further explored in the future.

8

Meinke-Carstanjen, Annette. "Aus Pflegeleitern werden Manager." kma - Klinik Management aktuell 10, no. 04 (April 2005): 94–95. http://dx.doi.org/10.1055/s-0036-1573264.

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Die Hochschule Bremen stellt den Diplom-Studiengang Internationale Pflegeleitung (ISP) auf Bachelor und Master um. Betriebswirtschaft und Management werden bei dem neuen Internationalen Studiengang Pflege- und Gesundheitsmanagement (ISPG) viel stärker im Visier stehen: Der Anteil erhöht sich um fast ein Drittel.

9

Rohdich, F., Stefan Hecht, Adelbert Bacher, and Wolfgang Eisenreich. "The deoxyxylulose phosphate pathway of isoprenoid biosynthesis. Discovery and function of the ispDEFGH genes and their cognate enzymes." Pure and Applied Chemistry 75, no. 2-3 (January 1, 2003): 393–405. http://dx.doi.org/10.1351/pac200375020393.

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Isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) serve as the universal precursors for the biosynthesis of terpenes. Besides the well-known mevalonate pathway, a second biosynthetic pathway conducive to IPP and DMAPP via 1-deoxy-d-xylulose-5-phosphate and 2C-methyl-d-erythritol-4-phosphate has been discovered recently in plants and certain eubacteria. 2C-Methyl-d-erythritol-4-phosphate, the first committed intermediate of the deoxyxylulose phosphate pathway, is converted into 2C-methyl-d-erythritol 2,4-cyclodiphosphate by the catalytic action of three enzymes specified by the ispDEF genes. The cyclic diphosphate is reductively opened by the IspG protein affording 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate. This compound can be converted into IPP as well as DMAPP by the catalytic action of IspH protein. The enzymes of this pathway are potential targets for novel antibacterial, antimalarial, and herbicide agents.

10

Nyland II, Rodney L., Youli Xiao, Pinghua Liu, and Caren L. Freel Meyers. "IspG Converts an Epoxide Substrate Analogue to (E)-4-Hydroxy-3-methylbut-2-enyl Diphosphate: Implications for IspG Catalysis in Isoprenoid Biosynthesis." Journal of the American Chemical Society 131, no. 49 (December 16, 2009): 17734–35. http://dx.doi.org/10.1021/ja907470n.

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11

Li, Qingyan, Feiyu Fan, Xiang Gao, Chen Yang, Changhao Bi, Jinlei Tang, Tao Liu, and Xueli Zhang. "Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli." Metabolic Engineering 44 (November 2017): 13–21. http://dx.doi.org/10.1016/j.ymben.2017.08.005.

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12

Wang, Weixue, and Eric Oldfield. "Biometallorganische Chemie mit IspG und IspH: Struktur, Funktion und Hemmung der an der Isoprenoid-Biosynthese beteiligten [Fe4S4]-Proteine." Angewandte Chemie 126, no. 17 (January 31, 2014): 4382–99. http://dx.doi.org/10.1002/ange.201306712.

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13

Wang, Weixue, and Eric Oldfield. "Bioorganometallic Chemistry with IspG and IspH: Structure, Function, and Inhibition of the [Fe4S4] Proteins Involved in Isoprenoid Biosynthesis." Angewandte Chemie International Edition 53, no. 17 (January 31, 2014): 4294–310. http://dx.doi.org/10.1002/anie.201306712.

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14

Yu, Wei Ling, Hanhong Dan, and Min Lin. "Novel protein targets of the humoral immune response to Listeria monocytogenes infection in rabbits." Journal of Medical Microbiology 56, no. 7 (July 1, 2007): 888–95. http://dx.doi.org/10.1099/jmm.0.46977-0.

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The role of the humoral immune response in protective immunity against listerial infection has been overlooked and is essentially unknown. This study aimed to discover the protein targets of Listeria monocytogenes that elicit an antibody response following infection in a rabbit model. A genomic expression library for L. monocytogenes was constructed and differentially screened to identify genes encoding proteins that reacted with antiserum from rabbits infected with live L. monocytogenes serotype 4b (RαL), but not with that from animals immunized with heat-killed bacteria (RαK). Thirty-one clones expressing proteins that reacted exclusively with RαL were identified and sequenced. Sequence analysis, together with Western blot analysis of the proteins expressed from positive clones, led to the identification of eight L. monocytogenes proteins as targets of humoral immune responses during listerial infection: three internalin members (InlA, InlD and InlC2) and five novel proteins of unknown function (designated IspA, IspB, IspC, IspD and IspE, respectively). Exhibition of humoral immune responses to these proteins in actively infected rabbits but not in animals receiving heat-killed L. monocytogenes suggested that they were induced or significantly upregulated in vivo during infection and thus are important in Listeria pathogenesis. With the exception of antibodies to InlA, this is the first demonstration of antibodies to the other seven proteins in infected hosts. These immunogenic proteins may be useful candidates for elucidation of the role of antibodies in protective immunity in the context of listerial infection, as well as potential targets for serodiagnostic reagents and vaccine and drug development.

15

Khan, Shaheen, Fei Wu, Shengxi Liu, Qian Wu, and Stephen Safe. "Role of specificity protein transcription factors in estrogen-induced gene expression in MCF-7 breast cancer cells." Journal of Molecular Endocrinology 39, no. 4 (October 2007): 289–304. http://dx.doi.org/10.1677/jme-07-0043.

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AbstractDeletion analysis of several 17β-estradiol (E2)-responsive genes have identified GC-rich sites that are associated with hormone-induced transactivation in MCF-7 breast cancer cells. However, the role of individual specificity proteins (Sps) in mediating hormone-induced gene expression has not been unequivocally determined. In transient transfection studies using E2-responsive GC-rich promoters from the E2F1, carbamoylphosphate synthetase/aspartate transcarbamylase/dihydroorotase (CAD), and retinoic acid receptor α (RARα) genes, RNA interference using small inhibitory RNAs for Sp1 (iSp1), Sp3 (iSp3), and Sp4 (iSp4) decreased both basal and E2-induced transactivation. The contributions of individual Sp proteins to basal and E2-induced activity were promoter dependent. iSp1, iSp3, and iSp4 also significantly inhibited hormonal induction of E2F1, CAD, and RARα mRNA levels; however, the enhanced inhibitory effects of the latter two small inhibitory RNAs suggest that Sp3 and Sp4 play a major role in estrogen receptor α/Sp-mediated gene expression in MCF-7 cells.

16

NAKAGAWA, Kazuya, Kentaro TAKADA, and Nobutaka IMAMURA. "Probable Novel MEP Pathway Inhibitor and Its Binding Protein, IspG." Bioscience, Biotechnology, and Biochemistry 77, no. 7 (July 23, 2013): 1449–54. http://dx.doi.org/10.1271/bbb.130094.

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17

Rekittke, Ingo, Hassan Jomaa, and Ulrich Ermler. "Structure of the GcpE (IspG)-MEcPP complex from Thermus thermophilus." FEBS Letters 586, no. 19 (August 9, 2012): 3452–57. http://dx.doi.org/10.1016/j.febslet.2012.07.070.

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18

Rohdich, F., F. Zepeck, P. Adam, S. Hecht, J. Kaiser, R. Laupitz, T. Grawert, et al. "The deoxyxylulose phosphate pathway of isoprenoid biosynthesis: Studies on the mechanisms of the reactions catalyzed by IspG and IspH protein." Proceedings of the National Academy of Sciences 100, no. 4 (February 5, 2003): 1586–91. http://dx.doi.org/10.1073/pnas.0337742100.

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19

Wang, Weixue, and Eric Oldfield. "ChemInform Abstract: Bioorganometallic Chemistry with IspG and IspH: Structure, Function, and Inhibition of the [Fe4S4] Proteins Involved in Isoprenoid Biosynthesis." ChemInform 45, no. 25 (June 5, 2014): no. http://dx.doi.org/10.1002/chin.201425288.

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20

Zepeck, Ferdinand, Tobias Gräwert, Johannes Kaiser, Nicholas Schramek, Wolfgang Eisenreich, Adelbert Bacher, and Felix Rohdich. "Biosynthesis of Isoprenoids. Purification and Properties of IspG Protein fromEscherichia coli." Journal of Organic Chemistry 70, no. 23 (November 2005): 9168–74. http://dx.doi.org/10.1021/jo0510787.

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21

Samani, Babak, Abbas Charchi, and Narges Khatib. "Strain and shortening amount analysis in the Asmari anticline, Khuzestan province." Iranian Journal of Petroleum Geology 11, no. 22 (June 8, 2023): 54–65. http://dx.doi.org/10.61186/ispg.40876.11.22.54.

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22

Ramshini, Mina, ٍٍElahe Sattari, Ali Bahrami, and Hosyen Vaziri moghadam. "Biostratigraphy, biofacies and CAI of the late Devonian deposits in Kale-Sardar section, North-east Tabas, based on conodont fauna." Iranian Journal of Petroleum Geology 11, no. 22 (June 8, 2023): 1–25. http://dx.doi.org/10.61186/ispg.41138.11.22.1.

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23

taherizade, somaye, Mohammad Vahidinia, and mohammadhossein mahmoudi gharaii. "Biostratigraphy and Lithostratigraphy of Pabdeh Formation is based on planktonic foraminifera in the section of Jahangirabad (south Ilam_ Zagros basin)." Iranian Journal of Petroleum Geology 11, no. 22 (June 8, 2023): 66–88. http://dx.doi.org/10.61186/ispg.40920.11.22.66.

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24

Motamedalshariati, Maryam, and Mahmoud Jalali. "Biostratigraphy and paleoecology of Family Gavelinellidae in Sanganeh and Aitamir Formations at the Kopeh-Dagh basin." Iranian Journal of Petroleum Geology 11, no. 22 (June 8, 2023): 89–108. http://dx.doi.org/10.61186/ispg.41339.11.22.89.

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25

Safaei-Farouji, Majid, Hossain Rahimpour-Bonab, and Buyuk Ghorbani. "Source rock characterization of the Fahlian, Gadvan, and Surmeh formations in giant Gachsaran oilfield." Iranian Journal of Petroleum Geology 11, no. 22 (June 8, 2023): 26–38. http://dx.doi.org/10.61186/ispg.33873.11.22.26.

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26

rezaie yegane doost, hossein. "Permeability improvement calculated from Stoneley-FZI method in Kangan reservoir, one of Iran's gas fields." Iranian Journal of Petroleum Geology 11, no. 22 (June 8, 2023): 39–53. http://dx.doi.org/10.61186/ispg.38526.11.22.39.

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27

Lee, Matthias, Tobias Gräwert, Felix Quitterer, Felix Rohdich, Jörg Eppinger, Wolfgang Eisenreich, Adelbert Bacher, and Michael Groll. "Biosynthesis of Isoprenoids: Crystal Structure of the [4Fe–4S] Cluster Protein IspG." Journal of Molecular Biology 404, no. 4 (December 2010): 600–610. http://dx.doi.org/10.1016/j.jmb.2010.09.050.

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28

Sabatello, Maya, Lea Davis, and Holly Peay. "ISPG ETHICS COMMITTEE SYMPOSIUM: RACE AND STRUCTURAL RACISM IN PSYCHIATRY AND PSYCHIATRIC GENETICS." European Neuropsychopharmacology 51 (October 2021): e24-e25. http://dx.doi.org/10.1016/j.euroneuro.2021.07.059.

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29

Wang, Weixue, Ke Wang, Jikun Li, Saritha Nellutla, Tatyana I. Smirnova, and Eric Oldfield. "An ENDOR and HYSCORE Investigation of a Reaction Intermediate in IspG (GcpE) Catalysis." Journal of the American Chemical Society 133, no. 22 (June 8, 2011): 8400–8403. http://dx.doi.org/10.1021/ja200763a.

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30

Thi Trung, Nguyen, Nguyen Thi Thao, Phan Thi Hong Thao, Tran Thanh Tuan, Pham Duy Nam, Le Thanh Hoang та Do Thi Tuyen. "Isolation, selection and evaluation of α-glucosidase inhibitory activity from endophytic Streptomyces sp. isolated from Citrus myrtifolia cultivar in Hoa Binh, Vietnam". Vietnam Journal of Biotechnology 20, № 4 (28 грудня 2022): 693–704. http://dx.doi.org/10.15625/1811-4989/17172.

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Cao Phong orange (Citrus sinensis Lour) was grown in Cao Phong district, Hoa Binh province. Citrus sinensis is Vietnam’s most valued commercial fruit. In this study, 30 microorganim strains were isolated from the samples of root, stem, and leaf of Cao Phong orange. The ratio of endophytic actinomycetes was found in different parts of the plant: root, stem, and leaf was 37%, 40%, and 23%, respectively. The isolating HBC6-2 was selected because of its high output of α-glucosidase inhibitors from 6 different strains. Morphological, physiological, and biochemical characterization showed that HBC6-2 strain belongs to the Streptomyces sp. The 16S rRNA sequence of HBC6-2 indicated 99% identity to the corresponding sequence of Streptomyces costaricanus, and was registered on GenBank with the code MT 453944.1. Streptomyces costaricanus EBL.HB6 was able to produce melanin yelow pigment, and its aerial and substrate mycelia have brown and yellow-grey pigment on ISP2, ISP3, ISP4, and ISP5 cultivating medium, respectively. The optimal pH range was from 5-10 and temperature from 15-40°C and exhibited salt tolerance up to 3% and utilized the carbon sources such as fructose, xylose, arabinose, cellulose, and rhamnose. In the following investigation, Streptomyces costaricanus EBL.HB6 displayed the highest α-glucosidase inhibitory activity by 68.98% among Streptomyces sp. strains

31

Sharma, Navneet, Jochen Fahr, Bernard Renaux, Mahmoud Saifeddine, Rajeev Kumar, Sandra Nishikawa, Koichiro Mihara, Rithwik Ramachandran, Morley D. Hollenberg, and Derrick E. Rancourt. "Implantation serine proteinase 2 is a monomeric enzyme with mixed serine proteolytic activity and can silence signalling via proteinase activated receptors." Biochemistry and Cell Biology 91, no. 6 (December 2013): 487–97. http://dx.doi.org/10.1139/bcb-2013-0006.

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Implantation serine proteinase 2 (ISP2), a S1 family serine proteinase, is known for its role in the critical processes of embryo hatching and implantation in the mouse uterus. Native implantation serine proteinases (ISPs) are co-expressed and co-exist as heterodimers in uterine and blastocyst tissues. The ISP1–ISP2 enzyme complex shows trypsin-like substrate specificity. In contrast, we found that ISP2, isolated as a 34 kDa monomer from a Pichia pastoris expression system, exhibited a mixed serine proteolytic substrate specificity, as determined by a phage display peptide cleavage approach and verified by the in vitro cleavage of synthetic peptides. Based upon the peptide sequence substrate selectivity, a database search identified many potential ISP2 targets of physiological relevance, including the proteinase activated receptor 2 (PAR2). The in vitro cleavage studies with PAR2-derived peptides confirmed the mixed substrate specificity of ISP2. Treatment of cell lines expressing proteinase-activated receptors (PARs) 1, 2, and 4 with ISP2 prevented receptor activation by either thrombin (PARs 1 and 4) or trypsin (PAR2). The disarming and silencing of PARs by ISP2 may play a role in successful embryo implantation.

32

Hecht, S., W. Eisenreich, P. Adam, S. Amslinger, K. Kis, A. Bacher, D. Arigoni, and F. Rohdich. "Studies on the nonmevalonate pathway to terpenes: The role of the GcpE (IspG) protein." Proceedings of the National Academy of Sciences 98, no. 26 (December 18, 2001): 14837–42. http://dx.doi.org/10.1073/pnas.201399298.

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33

Xiao, Youli, Georgia Zahariou, Yiannis Sanakis, and Pinghua Liu. "IspG Enzyme Activity in the Deoxyxylulose Phosphate Pathway: Roles of the Iron−Sulfur Cluster." Biochemistry 48, no. 44 (November 10, 2009): 10483–85. http://dx.doi.org/10.1021/bi901519q.

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34

Liu, Y. L., F. Guerra, K. Wang, W. Wang, J. Li, C. Huang, W. Zhu, et al. "Structure, function and inhibition of the two- and three-domain 4Fe-4S IspG proteins." Proceedings of the National Academy of Sciences 109, no. 22 (May 14, 2012): 8558–63. http://dx.doi.org/10.1073/pnas.1121107109.

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35

Wang, W., J. Li, K. Wang, C. Huang, Y. Zhang, and E. Oldfield. "Organometallic mechanism of action and inhibition of the 4Fe-4S isoprenoid biosynthesis protein GcpE (IspG)." Proceedings of the National Academy of Sciences 107, no. 25 (June 7, 2010): 11189–93. http://dx.doi.org/10.1073/pnas.1000264107.

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36

Xiao, Youli, Debra Rooker, Quincy You, Caren L. Freel Meyers, and Pinghua Liu. "IspG-Catalyzed Positional Isotopic Exchange in Methylerythritol Cyclodiphosphate of the Deoxyxylulose Phosphate Pathway: Mechanistic Implications." ChemBioChem 12, no. 4 (January 26, 2011): 527–30. http://dx.doi.org/10.1002/cbic.201000716.

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37

Trofimov, Leonid N., and Ivan L. Trofimov. "Optimization of Capacities of Wind and Solar Power Plants in the Interstate Power Grid in North-East Asia Taking Into Account the Intermittence of Their Power Output." E3S Web of Conferences 209 (2020): 04005. http://dx.doi.org/10.1051/e3sconf/202020904005.

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The paper considers the problems of optimization of wind and solar power plants (WPP and SPP) expansion and it operating modes in the prospective interstate power grid (ISPG) in Northeast Asia, taking into account the intermittence of their power output. A series of experiments with variations in the profiles of WPP and SPP in the model nodes were carried out. Also authors carried out some additional experiments with the increase in fuel costs for fossil fuel power plants due to environmental restrictions. As a result, an assessment of the character of the changes in the solution of the model for new installed capacities and their costs, depending on the variations for the profiles of the contributions generation of WPP and SPP to cover daily electrical load graphs, is obtained.

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Seemann, Myriam, Bernadette Tse Sum Bui, Murielle Wolff, Myroslawa Miginiac-Maslow, and Michel Rohmer. "Isoprenoid biosynthesis in plant chloroplasts via the MEP pathway: Direct thylakoid/ferredoxin-dependent photoreduction of GcpE/IspG." FEBS Letters 580, no. 6 (February 2, 2006): 1547–52. http://dx.doi.org/10.1016/j.febslet.2006.01.082.

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Schulze, Thomas G., Margit Burmeister, and Vishwajit Nimgaonkar. "Psychiatric Genetics Around The Globe - Chances And Challenges: An Update From The Global Diversity Task Force Of The ISPG." European Neuropsychopharmacology 29 (2019): S744. http://dx.doi.org/10.1016/j.euroneuro.2017.06.078.

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40

Quitterer, Felix, Annika Frank, Ke Wang, Guodong Rao, Bing O'Dowd, Jikun Li, Francisco Guerra, et al. "Atomic-Resolution Structures of Discrete Stages on the Reaction Coordinate of the [Fe 4 S 4 ] Enzyme IspG (GcpE)." Journal of Molecular Biology 427, no. 12 (June 2015): 2220–28. http://dx.doi.org/10.1016/j.jmb.2015.04.002.

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41

Mulia, Putra, Nofrizal Nofrizal, and Wan Nishfa Dewi. "ANALISIS DAMPAK KABUT ASAP KARHUTLA TERHADAP GANGGUAN KESEHATAN FISIK." Jurnal Ners Indonesia 12, no. 1 (October 7, 2021): 51. http://dx.doi.org/10.31258/jni.12.1.51-66.

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Kebakaran hutan dan lahan di Indonesia pada Tahun 2019 telah menyebabkan bencana kabut asap dan kota Pekanbaru menjadi salah satu wilayah yang terdampak. Penelitian ini bertujuan untuk menganalisis dampak kesehatan fisik dan kesehatan mental akibat bencana kabut asap. Data yang dikumpulkan adalah data harian ISPU dan kunjungan pasien ke Puskesmas dengan keluhan ISPA, asma, iritasi mata, iritasi kulit dan diare akibat kabut asap, selama periode kabut asap Tahun 2019. Metode analisis gangguan kesehatan fisik menggunakan Korelasi Spearman’s rho. Hasil penelitian menunjukkan ISPU tertinggi yaitu 374 (sangat tidak sehat). Hasil penelitian menunjukkan kadar ISPU terhadap penyakit asma (p = 0,000), ISPA (p = 0,000), pneumonia (p = 0,005), iritasi mata (p = 0,000), iritasi kulit (p = 0,001) dan diare (p = 0,002), sehingga dapat disimpulkan bahwa kadar ISPU berhubungan signifikan dengan gangguan kesehatan fisik. Berdasarkan penelitian dapat disarankan kepada peneliti selanjutnya, perlu melakukan studi cohort lanjutan terkait dampak kebakaran hutan dan lahan terhadap gangguan kesehatan fisik.

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Mulia, Putra, Nofrizal Nofrizal, and Wan Nishfa Dewi. "ANALISIS DAMPAK KABUT ASAP KARHUTLA TERHADAP GANGGUAN KESEHATAN FISIK." Jurnal Ners Indonesia 12, no. 1 (October 7, 2021): 51. http://dx.doi.org/10.31258/jni.12.1.51-66.

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Kebakaran hutan dan lahan di Indonesia pada Tahun 2019 telah menyebabkan bencana kabut asap dan kota Pekanbaru menjadi salah satu wilayah yang terdampak. Penelitian ini bertujuan untuk menganalisis dampak kesehatan fisik dan kesehatan mental akibat bencana kabut asap. Data yang dikumpulkan adalah data harian ISPU dan kunjungan pasien ke Puskesmas dengan keluhan ISPA, asma, iritasi mata, iritasi kulit dan diare akibat kabut asap, selama periode kabut asap Tahun 2019. Metode analisis gangguan kesehatan fisik menggunakan Korelasi Spearman’s rho. Hasil penelitian menunjukkan ISPU tertinggi yaitu 374 (sangat tidak sehat). Hasil penelitian menunjukkan kadar ISPU terhadap penyakit asma (p = 0,000), ISPA (p = 0,000), pneumonia (p = 0,005), iritasi mata (p = 0,000), iritasi kulit (p = 0,001) dan diare (p = 0,002), sehingga dapat disimpulkan bahwa kadar ISPU berhubungan signifikan dengan gangguan kesehatan fisik. Berdasarkan penelitian dapat disarankan kepada peneliti selanjutnya, perlu melakukan studi cohort lanjutan terkait dampak kebakaran hutan dan lahan terhadap gangguan kesehatan fisik.

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Nofiani, Risa, Rizky Rizky, and Ridho Brilliantoro. "Antibacterial Activities and Toxicity of Streptosporangium sp. SM1P." Molekul 16, no. 3 (November 15, 2021): 210. http://dx.doi.org/10.20884/1.jm.2021.16.3.780.

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This study aims to explore the anti-bacterial and toxicity activities from a rare actinobacterium isolated from mangrove, Mempawah District, West Kalimantan. The mangrove mud sample from Mempawah district was inoculated on ISP4 agar using a pour plate method. After 4 days of incubation, a colony of suspected actinobacterium was appeared, then isolated and coded as SM1P. SM1P was characterized based on morphological and biochemical traits and identified as a genus of Streptroporangium then called Streptroporangium sp. SM1P. Streptroporangium sp. SM1P was carried out anti-bacterial assay on both ISP1 agar and ISP4 agar media using the cross-streak method for the solid-state fermentation. The result showed that Streptroporangium sp. SM1P could inhibit Streptococcus sp. and Salmonella typhi on ISP1 agar and treptococcus sp., Escherichia coli, Vibrio cholerae, Staphylococcus aureus and Salmonella typhi on ISP4 agar. Streptroporangium sp. SM1P was cultivated on ISP1 broth and extracted using ethyl acetate, then evaporated to obtain crude extract. The crude extract was used for anti-bacterial assay (well-diffusion method for liquid-state fermentation) and toxicity assay (brine shrimp lethality test). The crude extract was active against 2 of the test bacteria (Streptococcus sp. and E. coli). The best medium and state fermentation for anti-bacterial assay were ISP4 agar with the condition of solid-state fermentation. The extract SM1P prepared on ISP1 broth showed toxic activity based on LC50 (106.094 µg/mL). Therefore, Streptroporangium sp. SM1P have a potential source to explore secondary metabolites having anti-bacterial and toxicity activities.

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Xiao, Youli, Debra Rooker, Quincy You, Caren L. Freel Meyers, and Pinghua Liu. "Inside Cover: IspG-Catalyzed Positional Isotopic Exchange in Methylerythritol Cyclodiphosphate of the Deoxyxylulose Phosphate Pathway: Mechanistic Implications (ChemBioChem 4/2011)." ChemBioChem 12, no. 4 (February 25, 2011): 494. http://dx.doi.org/10.1002/cbic.201190011.

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45

Putra Mulia, Nofrizal, and Wan Nishfa Dewi. "Analisis Dampak Kabut Asap Karhutla Terhadap Gangguan Kesehatan Fisik Dan Mental." HEALTH CARE : JURNAL KESEHATAN 10, no. 1 (June 26, 2021): 62–68. http://dx.doi.org/10.36763/healthcare.v10i1.103.

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Kebakaran hutan dan lahan di Indonesia pada Tahun 2019 telah menyebabkan bencana kabut asap dan kota Pekanbaru menjadi salah satu wilayah yang terdampak. Penelitian ini bertujuan untuk menganalisis dampak kesehatan fisik dan kesehatan mental akibat bencana kabut asap. Data yang dikumpulkan adalah data harian ISPU dan kunjungan pasien ke Puskesmas dengan keluhan ISPA, asma, iritasi mata, iritasi kulit dan diare akibat kabut asap, serta kunjungan pasien ke RSJ Tampan dengan stres, ansietas dan PSTD. Metode analisis gangguan kesehatan fisik menggunakan Korelasi Spearman’s rho dan gangguan kesehatan mental dianalisis dengan secara deskriptif. Hasil penelitian menunjukkan ISPU tertinggi yaitu 374 (sangat tidak sehat). Hasil penelitian menunjukkan kadar ISPU terhadap penyakit asma (p = 0,000), ISPA (p = 0,000), pneumonia (p = 0,005), iritasi mata (p = 0,000), iritasi kulit (p = 0,001) dan diare (p = 0,002), sehingga dapat disimpulkan bahwa kadar ISPU berhubungan signifikan dengan gangguan kesehatan fisik. Dari hasil penelitian, tidak ditemukan peningkatan kasus stres akut, kecemasan dan PTSD pada periode bencana kabut asap. Berdasarkan penelitian dapat disarankan kepada peneliti selanjutnya, perlu melakukan studi cohort lanjutan terkait dampak kebakaran hutan dan lahan terhadap risiko gangguan stres, kecemasan dan PTSD

46

Wu, Xiongting, Wenju Lu, Mengzhang He, Haixia Chen, Yuqin Chen, Xin Duan, Qiuyu Zheng, et al. "Structural and functional definition of the pulmonary vein system in a chronic hypoxia-induced pulmonary hypertension rat model." American Journal of Physiology-Cell Physiology 318, no. 3 (March 1, 2020): C555—C569. http://dx.doi.org/10.1152/ajpcell.00289.2019.

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Unlike the pulmonary artery (PA), the pathophysiological changes of the pulmonary vein (PV) in the development of pulmonary hypertension (PH) remain largely unknown. In this study, we comprehensively investigated the structural and functional changes in the PV isolated from the chronic hypoxia (CH; 10% O2, 21 days)-induced PH rat model (CHPH). Results showed that CH caused an increase in right ventricular pressure but did not affect the mean pulmonary venous pressure and the left atrial pressure. Similar to the PA, vascular lumen stenosis and medial thickening were also observed in the intrapulmonary veins isolated from the CHPH rats. Notably, CH induced more severe loss in the endothelium of intrapulmonary veins than the arteries. Then, the contractile response to 5-HT and U46619 was significantly greater in the intrapulmonary small veins (ISPV) and arteries (ISPA) isolated from CHPH rats than those from normoxic rats but not in the extrapulmonary and intrapulmonary large veins. Treatment with nifedipine (Nif), SKF96365 (SKF), or ryanodine and caffeine either partially attenuated (Nif) or dramatically abolished (SKF or ryanodine and caffeine) 5-HT-induced maximal contraction in ISPV from both normoxic and CHPH rats. Because of the severe loss of endothelium in the PV of CHPH rats, the decrease in acetylcholine (ACh)-induced endothelium-dependent relaxation was significantly larger in ISPV than ISPA, whereas the sodium nitroprusside-induced endothelium-independent relaxation was not altered in both ISPA and ISPV. In conclusion, our results provide fundamental data to comprehensively define the PV system in CHPH rat model.

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Ratte, Marina, Irmanida Batubara, and Yulin Lestari. "Morphological Characterization and Antioxidant Activity of Actinobacteria from Xylocarpus granatum Growing in Mangrove Habitat." Biotropika: Journal of Tropical Biology 10, no. 1 (March 30, 2022): 1–10. http://dx.doi.org/10.21776/ub.biotropika.2022.010.01.01.

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Xylocarpus granatum produces various bioactive compounds with diverse biological activities, one of them as an antioxidant. Endophytic actinobacteria can also produce bioactive compounds. This study aimed to explore the existence of the endophytic actinobacteria of X. granatum fruit and determine its antioxidant activity. The research method was carried out by isolating the endophytic actinobacteria from the fruit using selective media Humic Acid (HV). Furthermore, characterizing the obtained isolates using four different media, namely YSA, ISP2, ISP3, and ISP4, measured antioxidant activity for actinobacterial supernatants and actinobacterial ethyl acetate extracts using the DPPH method. The results showed that from X. granatum, 15 isolates of endophytic actinobacteria were obtained from fruit, seeds, and leaves. Endophytic actinobacteria isolates from X. granatum showed different colors of aerial mycelium, substrate mycelium, and spore chain type. XGF11, XGF12, and XGF4 isolates produced high antioxidant activity of the supernatant; 57.30%, 49.77%, and 41.90% inhibition, respectively. The antioxidant capacity of XGF12 ethyl acetate extract was 501.60 μg AEAC/mg extract and 465.47 μg AEAC/mg extract for XGF11 ethyl acetate extract. Based on 16S rRNA gene analysis, isolates XGF12 and XGF11 showed similarities with Streptomyces xylanilyticus, and isolated XGF4 showed similarities with Brevibacterium sediminis.

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Xu, Weiya, Nicholas S. Lees, Dolapo Adedeji, Jochen Wiesner, Hassan Jomaa, Brian M. Hoffman, and Evert C. Duin. "Paramagnetic Intermediates of (E)-4-Hydroxy-3-methylbut-2-enyl Diphosphate Synthase (GcpE/IspG) under Steady-State and Pre-Steady-State Conditions." Journal of the American Chemical Society 132, no. 41 (October 20, 2010): 14509–20. http://dx.doi.org/10.1021/ja101764w.

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49

Saggu, Gagandeep Singh, Shilpi Garg, Zarna Rajeshkumar Pala, Sushil Kumar Yadav, Sanjay Kumar Kochar, Dhanpat Kumar Kochar, and Vishal Saxena. "Characterization of 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (IspG) from Plasmodium vivax and it’s potential as an antimalarial drug target." International Journal of Biological Macromolecules 96 (March 2017): 466–73. http://dx.doi.org/10.1016/j.ijbiomac.2016.12.033.

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50

SA Mir, S. Akhter, S. S. Ahmad, S. H. Altaf, J. Ahmad, N. Jehan, and Asmaa. "To Study the Biochemical Markers for the Prediction of Fistula Formation after Pancreatic Resection Surgery in the Post Operative Period." Academia Journal of Surgery 3, no. 2 (January 30, 2021): 33–39. http://dx.doi.org/10.47008/ajs/2020.3.2.9.

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Background: To study and assess the potential laboratory biomarkers for foreseeing postoperative pancreatic fistula (POPF) after pancreatic resection medical procedures. Subjects and Methods: 64 patients were studied from Jan 2008 to Dec 2018. All patients undergoing Pancreatic resection surgeries like Pancreaticoduodenectomy (PD), distal Pancreatectomy (DP) for tumors of the periampullary region, Pancreatic body or chronic pancreatitis Clinical data of the patients, surgical approach, pancreatic parenchymal tissue consistency, histopathology of the specimen, white blood cell count and blood amylase levels tube Drain fluid amylase were recorded and analyzed. Correlations between these parameters and postoperative pancreatic fistula (POPF) were assessed. Results : 22 out of the 64 (34.37%) patients developed POPF. The fistula was graded according to the ISPG classification. It was Grade An of every 10 (45.45%) patients, grade B in 8 (36.36%), and grade C in 4 (18.18%),.On univariate and multivariate strategic relapse investigations, higher amylase levels in the stomach waste liquid on a postoperative day (POD)1 and higher serum amylase levels on POD4 indicated a critical relationship with fistula arrangement in the post-operation period (POPF) (P < 0.05). On ROC (recipient working trademark bend) examination, amylase cut-off degree of 2270.67 U/L in the stomach waste liquid was related with a 76.6% affectability and 82% particularity [area under the bend (AUC): 0.844; P = 0.009]. A cut-off serum amylase level of 53.2 U/L was related with a 74.6% affectability and 72.9% explicitness (AUC: 0.784; P = 0.05). Conclusion: Post-operative channel liquid amylase levels on day 1 and serum amylase level on day 4 speak to interesting biomarkers related to POPF improvement after the pancreatic medical procedure.