Добірка наукової літератури з теми "Isolateurs (génétique)"
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Статті в журналах з теми "Isolateurs (génétique)"
Belaganahalli, M., S. Maan, and P. P. C. Mertens. "Caractérisation génétique des virus Tilligerry et Mitchell River." Revue d’élevage et de médecine vétérinaire des pays tropicaux 62, no. 2-4 (February 1, 2009): 151. http://dx.doi.org/10.19182/remvt.10060.
Повний текст джерелаRenault, Lionel, Didier Mugniéry, Michel Bossis, and Christophe Tastet. "Protein variation in tropical Meloidogyne spp. as shown by two-dimensional electrophoregram computed analysis." Nematology 2, no. 3 (2000): 343–53. http://dx.doi.org/10.1163/156854100509105.
Повний текст джерелаObala, T., S. O. Arojjo, M. Afayoa, K. Ikwap, and J. Erume. "The role of Escherichia coli in the etiology of piglet diarrhea in selected pig producing districts of central Uganda." African Journal of Clinical and Experimental Microbiology 22, no. 4 (September 27, 2021): 515–25. http://dx.doi.org/10.4314/ajcem.v22i4.12.
Повний текст джерелаAjayi, A. A., G. O. Onipede, B. C. Okafor, K. A. Adepoju, and J. C. Nwabuenu. "Phenotypic identification of soil bacterial and fungal communities inhabiting an archaeological monument at Augustine University, Ilara Epe, southwest Nigeria." African Journal of Clinical and Experimental Microbiology 22, no. 4 (September 27, 2021): 473–79. http://dx.doi.org/10.4314/ajcem.v22i4.7.
Повний текст джерелаДисертації з теми "Isolateurs (génétique)"
Heurteau, Alexandre. "Etude bioinformatique intégrative : déterminants et dynamique des interactions chromosomiques à longue distance." Electronic Thesis or Diss., Toulouse 3, 2019. http://www.theses.fr/2019TOU30343.
Повний текст джерелаInsulator Binding Proteins (IBPs) could be involved in the three-dimensional folding of genomes into topological domains (or "TADs"). In particular, TADs would help to separate the inactive/heterochromatin and active/euchromatin compartments. IBPs are also able to block specific contacts between the activator or enhancer elements of one TAD and target gene promoters present in another TAD. Thus, insulators may influence gene expression according to several regulatory modes that have yet to be characterized at genome level. The results obtained in the first part of my thesis show how IBPs influence gene expression according to a new regulatory mechanism, as shown at the scale of the Drosophila genome. Our bioinformatics analyses show that IBPs regulate the spread of repressive heterochromatin (H3K27me3) both in cis and trans. Trans regulations involve chromatin loops between insulators positioned at the heterochromatin boundary and distant insulators positioned at the edges of euchromatic genes. Trans spreading leads to the formation of "micro-domains" of heterochromatin, thereby repressing distant genes. In particular, an insulator mutant that prevents loop formation significantly reduces the establishment of micro-domains. In addition, these micro-domains would be formed during development suggesting a new insulator-dependent mechanism for gene regulation. Furthermore, we could uncover a novel function of cohesion, a key regulator of 3D loops in humans, in regulating non-coding RNAs (ncRNAs), including "PROMoters uPstream Transcripts" (PROMPTs) and enhancers RNAs (eRNAs). The MTR4 helicase is essential to the control of coding and noncoding RNA stability by the human nuclear-exosome targeting (NEXT) complex and pA-tail exosome targeting (PAXT) complex. Remarkably, ncRNAs could be detected upon depletion of the Mtr4 helicase of the human NEXT complex. Moreover, depletion of additional NEXT subunits, ZFC3H1 and ZCCHC8 (or Z1 and Z8), also led to uncover ncRNAs often produced from the same loci as upon MTR4 depletion. Curiously however, mapping of Mtr4 binding sites highlighted that Mtr4 binds to sites that are distant from PROMPTs. Rather than acting in cis, our data suggest that regulation of PROMPTs could involve specific long-distance contacts between these distant MTR4 binding sites and promoters bound by Z1/Z8. As such, integration of Hi-C data together with the detection of PROMPTS upon MTR4-, Z1- or Z8- depletions highlight possible role of long-range interactions in regulating PROMPTs, from distant MTR4-bound sites. This work may establish a new relationship between the 3D structure of genomes and the regulation of ncRNAs
Cottalorda, Agnès. "Diversité phénotypique et moléculaire d'isolats urinaires de Pseudomonas aeruginosa Antimicrobial resistance and genotypic diversity of Pseudomonas aeruginosa urinary isolates collected prospectively in a French hospital Within-host microevolution of Pseudomonas aeruginosa urinary isolates : a seven-patient longitudinal genomic and phenotypic study." Thesis, Normandie, 2020. http://www.theses.fr/2020NORMR028.
Повний текст джерелаThe aim of this study was to explore phenotypic (antimicrobial resistance) and genetic diversity (MultiLocus Sequence Typing and Whole Genome Sequencing) of P. aeruginosa urinary isolates. Thus, we have studied (i) within-sample diversity (several isolates analyzed per urine sample) of urinary isolates collected from 120 patients with urinary tract infection (40) or asymptomatic bacteriuria (80), and (ii) P. aeruginosa within-host evolution for 7 patients with successive urine samples (from 48 to 488 days apart). Even though patients were mostly colonized or infected by a given clone, this is the first study to identify within-sample phenotypic (28% of patients) and genotypic (4% of patients) diversity, and thus polyclonal bacteriuria. P. aeruginosa adaptation to the urinary tract was mostly driven by mutations and/or large genomic deletions, particularly in genes encoding transcriptional regulators, two component systems and carbon compound catabolism. Genomic adaptation was associated with phenotypic changes in terms of biofilm formation and fitness.Thus, phenotypic and genetic within-host diversity should be taken into account in the diagnosis and treatment of P. aeruginosa urinary tract infections
Baquero, Uriza Diana Paola. "Isolation and characterization of hyperthermophilic archaeal viruses." Electronic Thesis or Diss., Sorbonne université, 2020. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2020SORUS079.pdf.
Повний текст джерелаExtreme geothermal environments are inhabited by archaeal viruses with unique genome contents and remarkable morphologies, many of which have not been described among viruses infecting bacteria or eukaryotes. However, the number of known species of viruses infecting archaea remains low compared to the eukaryotic or bacterial viruses. Moreover, the lack of relationships to other known viruses and distinctiveness of their genomes suggest that the mechanisms of virus-host interaction are likely to be also novel. Therefore, in the framework of my studies, I have focused on two major lines of research: (i) isolation and characterization of new hyperthermophilic archaeal viruses and (ii) molecular mechanisms of virus-host interactions in Archaea. We investigated the virus diversity in the sulfurous fields of the Campi Flegrei volcano in Pozzuoli, Italy. Five new archaeal viruses infecting neutrophilic hyperthermophiles of the genus Pyrobaculum and acidophilic hyperthermophiles belonging to three different genera of the order Sulfolobales, namely, Saccharolobus, Acidianus, and Metallosphaera were isolated. The newly characterized viruses belong to the families Rudiviridae, Globuloviridae and Tristromaviridae. Notably, phylogenomic analysis of the newly isolated and previously sequenced rudiviruses revealed a clear biogeographic pattern, with all Italian rudiviruses forming a monophyletic clade, suggesting geographical structuring of virus communities in extreme geothermal environments. One of the non-enveloped rudiviruses isolated in Pozzuoli, namely, Saccharolobus solfataricus rod-shaped virus 1 (SSRV1), and the enveloped Sulfolobus islandicus filamentous virus (SIFV) were structurally and biochemically characterized. The study has revealed conserved structural features shared by these viruses and clarified the evolutionary relationship between non-enveloped and enveloped filamentous viruses. The second line of research focused on understanding the mechanisms of virion assembly and release on the example of the enveloped filamentous virus SIFV, which infects the hyperthermophilic and acidophilic archaeon Sulfolobus islandicus. Our results showed that SIFV is a lytic virus, which is released through pyramidal portals formed in the host cell membrane, a highly unexpected egress mechanism for an enveloped virus. Interestingly, dual-axis electron tomography revealed that SIFV virions acquire their lipid envelopes inside the host through an unknown mechanism, involving either de novo membrane formation or trafficking of lipids from the cytoplasmic membrane to virion assembly centers
Herzig, Anthony Francis. "Studying the genetic architecture of complex traits in a population isolate." Thesis, Sorbonne Paris Cité, 2019. http://www.theses.fr/2019USPCC110.
Повний текст джерелаMy thesis project is concerned with tapping the potential of population isolates for the dissection of complex trait architecture. Specifically, isolates can aid the identification of variants that are usually rare in other populations. This thesis principally contains in depth investigations into genetic imputation and heritability analysis in isolates. We approached both of these studies from two main angles; first from a methodological standpoint where we created extensive simulation datasets in order to investigate how the specificities of an isolate should determine strategies for analyses. Secondly, we demonstrated such concepts through analysis of genetic data in the known isolate of Cilento. Imputation is a crucial step to performing association analyses in an isolate and represents a cost-efficient method for gaining dense genetic data for the population. The effectiveness of imputation is of course dependent on its accuracy. Hence, we investigated the wide range of possible strategies to gain maximal imputation accuracy in an isolate. We showed that software using algorithms which specifically evoke known characteristics of isolates were, unexpectedly, not as successful as those designed for general populations. We also demonstrated a very small study specific imputation reference panel performing very strongly in an isolate; particularly for rare variants. For many complex traits, there exist discordances between estimates of heritabilities from studies in closely related individuals and from studies on unrelated individuals. In particular, we noted that most researchers consider dominant (non-additive) genetic effects as unlikely to play a significant role despite contrasting results from previous studies on isolates. Our second analysis revealed possible mechanisms to explain such disparate published heritability estimates between isolated populations and general populations. This allowed us to make interesting deductions from our own heritability analyses of the Cilento dataset, including an indication of a non-null dominance component involved in the distribution of low-density lipoprotein level measurements (LDL). This led us to perform genome-wide association analyses of additive and non-additive components for LDL in Cilento and we were able to identify genes that had been previously linked to the trait in other studies. In the contexts of both of our studies, we observed the importance of retaining genotype uncertainty (genotype dosage following imputation or genotype likelihoods from sequencing data). As a prospective of this thesis, we have proposed ways to incorporate this uncertainty into certain methods used in this project. Our findings for imputation strategies and heritability analysis will be highly valuable for the continued study of the isolate of Cilento but will also be instructive to researchers working on other isolated populations and also applicable to the study of complex diseases in general
Al, Bayssari Charbel. "Etude des mécanismes moléculaires de la résistance aux antibiotiques dans le bassin méditerranéen." Thesis, Aix-Marseille, 2015. http://www.theses.fr/2015AIXM5028.
Повний текст джерелаThe detection, monitoring and dissemination of bacterial resistance to antibiotics are a major issue worldwide since the discovery and spread of multi-resistant bacteria, in particular resistance to carbapenems, specifically among Enterobacteriaceae and bacteria of the genus Pseudomonas and Acinetobacter.The emergence and dissemination of carbapenem-resistant Gram-negative pathogens is a significant contributor to patient morbidity and mortality. Despite radical efforts in infection control and improvements in molecular diagnostics, carbapenem-resistant Gram-negative bacilli remain a formidable threat as few antimicrobial agents are reliably active and very little is expected to be available in the near future.The origin and source of resistance genes in the world are not well known and recent works suggest that domestic and wild animals, the environment (soil, water, rivers ..) but also the digestive tract of mammals and humans could represent a reservoir and an important source of resistance genes that may be transmissible to humans.It is in this context that this thesis project articulates with the following objectives: (i) The achievement of molecular epidemiological studies on carbapenem-resistant clinical and animal isolates collected from countries in the Mediterranean basin (Lebanon, Libya, France) and the characterization of the genetic determinants of this resistance; (ii) the description of new resistance mechanisms to imipenem; and finally (iii) The genome sequencing of clinical isolates resistant to carbapenems, the analysis of these genomes and the identification of mechanisms and genetic supports of the resistance to carbapenems and other antibiotics
Diene, Seydina Mouhamadou. "Analyse génomique et moléculaire d'isolats cliniques de bactéries multi-résistantes aux antibiotiques." Thesis, Aix-Marseille, 2012. http://www.theses.fr/2012AIXM5049.
Повний текст джерелаThe increase and spread of multidrug-resistant (MDR) gram-negative bacteria especially Enterobacteriaceae, Pseudomonas, and Acinetobacter (E.P.A) species have become a major concern worldwide. The hospital-acquired infections caused by MDR bacteria have led not only to an increase in mortality, morbidity, and cost of treatment, but also continue to endanger the life of patients, especially those immunocompromised. Although the frequent misuse of antibiotic drug has greatly contributed to worldwide dissemination and resistance to antibiotics; recent studies have shown that these resistance determinants could emerge from ancient or environmental sources. Front of this worldwide concern, several studies have been reported with significant recommendations to conduct molecular epidemiology, and genomic studies, in order to control the increase and the dissemination of the antibiotic resistance. Moreover, during these last 10 years, we are witnessing the emergence and development of new technologies of high throughput sequencing and coinciding with an exponential increase of number of bacterial genomes sequenced today. Therefore, it is in this context that the project of this thesis was conducted with three essential objectives: (i) the genome sequencing of clinical MDR bacteria, the analysis and the identification of the mechanisms and the genetic determinants of antimicrobial resistance (ii) the achievement of molecular epidemiology studies from clinical MDR bacteria responsible of outbreak (iii) the development and implementation of molecular tools for monitoring and diagnosis of potential MDR bacteria