Готові списки джерел за темами / Intrinsic UV fluorescence

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Добірка наукової літератури з теми "Intrinsic UV fluorescence"

Автор: Grafiati

Опубліковано: 8 липня 2022

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Статті в журналах з теми "Intrinsic UV fluorescence"

Bhartia, Rohit, Everett C. Salas, William F. Hug, Ray D. Reid, Arthur L. Lane, Katrina J. Edwards, and Kenneth H. Nealson. "Label-Free Bacterial Imaging with Deep-UV-Laser-Induced Native Fluorescence." Applied and Environmental Microbiology 76, no. 21 (September 3, 2010): 7231–37. http://dx.doi.org/10.1128/aem.00943-10.

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ABSTRACT We introduce a near-real-time optical imaging method that works via the detection of the intrinsic fluorescence of life forms upon excitation by deep-UV (DUV) illumination. A DUV (<250-nm) source enables the detection of microbes in their native state on natural materials, avoiding background autofluorescence and without the need for fluorescent dyes or tags. We demonstrate that DUV-laser-induced native fluorescence can detect bacteria on opaque surfaces at spatial scales ranging from tens of centimeters to micrometers and from communities to single cells. Given exposure times of 100 μs and low excitation intensities, this technique enables rapid imaging of bacterial communities and cells without irreversible sample alteration or destruction. We also demonstrate the first noninvasive detection of bacteria on in situ-incubated environmental experimental samples from the deep ocean (Lo'ihi Seamount), showing the use of DUV native fluorescence for in situ detection in the deep biosphere and other nutrient-limited environments.

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Abd Halim, Adyani Azizah, Mohammed Suleiman Zaroog, Habsah Abdul Kadir та Saad Tayyab. "Molten Globule-Like Partially Folded State ofBacillus licheniformis α-Amylase at Low pH Induced by 1,1,1,3,3,3-Hexafluoroisopropanol". Scientific World Journal 2014 (2014): 1–9. http://dx.doi.org/10.1155/2014/824768.

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Effect of 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) on acid-denaturedBacillus licheniformis α-amylase (BLA) at pH 2.0 was investigated by far-UV CD, intrinsic fluorescence, and ANS fluorescence measurements. Addition of increasing HFIP concentrations led to an increase in the mean residue ellipticity at 222 nm (MRE222 nm) up to 1.5 M HFIP concentration beyond which it sloped off. A small increase in the intrinsic fluorescence and a marked increase in the ANS fluorescence were also observed up to 0.4 M HFIP concentration, both of which decreased thereafter. Far- and near-UV CD spectra of the HFIP-induced state observed at 0.4 M HFIP showed significant retention of the secondary structures closer to native BLA but a disordered tertiary structure. Increase in the ANS fluorescence intensity was also observed with the HFIP-induced state, suggesting exposure of the hydrophobic clusters to the solvent. Furthermore, thermal denaturation of HFIP-induced state showed a non-cooperative transition. Taken together, all these results suggested that HFIP-induced state of BLA represented a molten globule-like state at pH 2.0.

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Meyer, Arne, Christian Betzel, and Marc Pusey. "Latest methods of fluorescence-based protein crystal identification." Acta Crystallographica Section F Structural Biology Communications 71, no. 2 (January 28, 2015): 121–31. http://dx.doi.org/10.1107/s2053230x15000114.

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Successful protein crystallization screening experiments are dependent upon the experimenter being able to identify positive outcomes. The introduction of fluorescence techniques has brought a powerful and versatile tool to the aid of the crystal grower. Trace fluorescent labeling, in which a fluorescent probe is covalently bound to a subpopulation (<0.5%) of the protein, enables the use of visible fluorescence. Alternatively, one can avoid covalent modification and use UV fluorescence, exploiting the intrinsic fluorescent amino acids present in most proteins. By the use of these techniques, crystals that had previously been obscured in the crystallization drop can readily be identified and distinguished from amorphous precipitate or salt crystals. Additionally, lead conditions that may not have been obvious as such under white-light illumination can be identified. In all cases review of the screening plate is considerably accelerated, as the eye can quickly note objects of increased intensity.

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Gagnon, Pete, Blaz Goricar, Nina Mencin, Timotej Zvanut, Sebastijan Peljhan, Maja Leskovec, and Ales Strancar. "Multiple-Monitor HPLC Assays for Rapid Process Development, In-Process Monitoring, and Validation of AAV Production and Purification." Pharmaceutics 13, no. 1 (January 17, 2021): 113. http://dx.doi.org/10.3390/pharmaceutics13010113.

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HPLC is established as a fast convenient analytical technology for characterizing the content of empty and full capsids in purified samples containing adeno-associated virus (AAV). UV-based monitoring unfortunately over-estimates the proportion of full capsids and offers little value for characterizing unpurified samples. The present study combines dual-wavelength UV monitoring with intrinsic fluorescence, extrinsic fluorescence, and light-scattering to extend the utility of HPLC for supporting development of therapeutic AAV-based drugs. Applications with anion exchange (AEC), cation exchange (CEC), and size exclusion chromatography (SEC) are presented. Intrinsic fluorescence increases sensitivity of AAV detection over UV and enables more objective estimation of empty and full capsid ratios by comparison of their respective peak areas. Light scattering enables identification of AAV capsids in complex samples, plus semiquantitative estimation of empty and full capsid ratios from relative peak areas of empty and full capsids. Extrinsic Picogreen fluorescence enables semiquantitative tracking of DNA with all HPLC methods at all stages of purification. It does not detect encapsidated DNA but reveals DNA associated principally with the exteriors of empty capsids. It also enables monitoring of host DNA contamination across chromatograms. These enhancements support many opportunities to improve characterization of raw materials and process intermediates, to accelerate process development, provide rapid in-process monitoring, and support process validation.

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Pottier, Fabien, Anne Michelin, Christine Andraud, Fabrice Goubard, and Bertrand Lavédrine. "Characterizing the Intrinsic Fluorescence Properties of Historical Painting Materials: The Case Study of a Sixteenth-Century Mesoamerican Manuscript." Applied Spectroscopy 72, no. 4 (December 14, 2017): 573–83. http://dx.doi.org/10.1177/0003702817747276.

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Ultraviolet visible (UV–Vis) fluorescence spectroscopy is widely used to study polychrome objects and can help to identify the nature of certain materials when they present specific fluorescent properties. However, given the complexity of the stratified and heterogeneous materials under study, the characterization of an intrinsic fluorescence related to a given constituent (a pigment or a binder composing a paint layer for example) is not straightforward, and the recorded raw data need to be corrected for a number of effects that can influence the detected spectral distribution. The application of standard correction procedures to experimental fluorescence data gathered on the polychromatic surface of the Codex Borbonicus, a 16th-century Aztec manuscript, is described. The results are confronted to an alternate new methodology that is based on the hypothesis of transparent non-scattering paint layers. This second approach allows to establish more clearly the material origin of the detected emission and to discriminate apparent fluorescence (emitted by the substrate and transmitted through the paint layers) from actual intrinsic emission generated by the coloring materials under study. The results show that most of the various emission profiles detected in the paint layers of the manuscript actually originate from a unique fluorophore (composing the substrate) and should not be used to characterize the coloring materials.

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Huang, Lei, Lianzhi Li, Haili Li, Chaohui Gao, Hui Cui, and Xiangshi Tan. "Multispectroscopic Study of the Interaction of Chloramphenicol with Human Neuroglobin." Spectroscopy: An International Journal 27 (2012): 143–54. http://dx.doi.org/10.1155/2012/192591.

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The interaction between chloramphenicol (CHL) and neuroglobin (Ngb) has been investigated by using fluorescence, synchronous fluorescence, UV-Vis and circular dichroism (CD) spectroscopy. It has been found that CHL molecule can quench the intrinsic fluorescence of Ngb in a way of dynamic quenching mechanism, which was supported by UV-Vis spectral data. Their effective quenching constants (KSV) are2.2×104,2.6×104,and 3.1×104 L⋅mol−1at 298 K, 303 K, and 308 K, respectively. The enthalpy change (ΔH) and entropy change (ΔS) for this reaction are 26.42 kJ⋅mol−1and 171.7 J⋅K−1, respectively. It means that the hydrophobic interaction is the main intermolecular force of the interaction between CHL and Ngb. Synchronous fluorescence spectra showed that the microenvironment of tryptophan and tyrosine residues of Ngb has been changed slightly. The fluorescence quenching efficiency of CHL to tyrosine residues is a little bit more than that to tryptophan residues of Ngb. Furthermore, CD spectra indicated that CHL can induce the formation of α-helix of Ngb.

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Jamme, Frederic, Sandrine Villette, Alexandre Giuliani, Valerie Rouam, Frank Wien, Bruno Lagarde, and Matthieu Réfrégiers. "Synchrotron UV Fluorescence Microscopy Uncovers New Probes in Cells and Tissues." Microscopy and Microanalysis 16, no. 5 (August 25, 2010): 507–14. http://dx.doi.org/10.1017/s1431927610093852.

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AbstractUse of deep ultraviolet (DUV, below 350 nm) fluorescence opens up new possibilities in biology because it does not need external specific probes or labeling but instead allows use of the intrinsic fluorescence that exists for many biomolecules when excited in this wavelength range. Indeed, observation of label free biomolecules or active drugs ensures that the label will not modify the biolocalization or any of its properties. In the past, it has not been easy to accomplish DUV fluorescence imaging due to limited sources and to microscope optics. Two worlds were coexisting: the spectrofluorometric measurements with full spectrum information with DUV excitation, which lacked high-resolution localization, and the microscopic world with very good spatial resolution but poor spectral resolution for which the wavelength range was limited to 350 nm. To combine the advantages of both worlds, we have developed a DUV fluorescence microscope for cell biology coupled to a synchrotron beamline, providing fine tunable excitation from 180 to 600 nm and full spectrum acquired on each point of the image, to study DUV excited fluorescence emitted from nanovolumes directly inside live cells or tissue biopsies.

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Xue, Mao-Yun, Ai-Ping Yang, Mei-Hua Ma, and Xiao-Hua Li. "The application of two-dimensional fluorescence correlation spectroscopy on the interaction between bovine serum albumin and prulifloxacin." Spectroscopy 23, no. 5-6 (2009): 257–63. http://dx.doi.org/10.1155/2009/565173.

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The interaction between bovine serum albumin (BSA) and prulifloxacin was investigated by ultraviolet spectrophotometer (UV) and fluorescence spectroscopy in this paper. Two-dimensional (2D) correlation spectroscopy was applied to the analysis of fluorescence spectra. The results of spectroscopic measurements suggested that prulifloxacin (PL) have a strong ability to quench the intrinsic fluorescence of bovine serum albumin through static quenching procedure. Thermodynamic parameter enthalpy changes (ΔH) and entropy changes (ΔS) were calculated. Owing to the spectral resolution enhancement in 2D correlation spectroscopy, the structure change of prulifloxacin can be observed.

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Lukk, Tiit, Richard E. Gillilan, Doletha M. E. Szebenyi, and Warren R. Zipfel. "A visible-light-excited fluorescence method for imaging protein crystals without added dyes." Journal of Applied Crystallography 49, no. 1 (February 1, 2016): 234–40. http://dx.doi.org/10.1107/s160057671502419x.

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Fluorescence microscopy methods have seen an increase in popularity in recent years for detecting protein crystals in screening trays. The fluorescence-based crystal detection methods have thus far relied on intrinsic UV-inducible tryptophan fluorescence, nonlinear optics or fluorescence in the visible light range dependent on crystals soaked with fluorescent dyes. In this paper data are presented on a novel visible-light-inducible autofluorescence arising from protein crystals as a result of general stabilization of conjugated double-bond systems and increased charge delocalization due to crystal packing. The visible-light-inducible autofluorescence serves as a complementary method to bright-field microscopy in beamline applications where accurate crystal centering about the rotation axis is essential. Owing to temperature-dependent chromophore stabilization, protein crystals exhibit tenfold higher fluorescence intensity at cryogenic temperatures, making the method ideal for experiments where crystals are cooled to 100 K with a cryostream. In addition to the non-damaging excitation wavelength and low laser power required for imaging, the method can also serve a useful role for differentiating protein crystals from salt crystals in screening trays.

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Abdelhameed, Ali Saber. "Insight into the Interaction between the HIV-1 Integrase Inhibitor Elvitegravir and Bovine Serum Albumin: A Spectroscopic Study." Journal of Spectroscopy 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/435674.

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The interaction between the anti-HIV drug Elvitegravir (EVG) and bovine serum albumin (BSA) was investigated by fluorescence spectroscopy and UV-visible absorption spectra. The mechanism for quenching the fluorescence of BSA by EVG is discussed. It was found that EVG can quench the intrinsic fluorescence of BSA through a static quenching procedure. The quenching type, association constant, and number of binding sites were investigated. The binding constant of EVG with BSA was calculated at different temperatures based on fluorescence quenching results. The thermodynamic parametersΔHθ,ΔGθ, andΔSθwere determined. The positiveΔSθand negativeΔHθandΔGθvalues showed that a spontaneous interaction may involve both roles of hydrophobic interaction and hydrogen bonding. The interaction of BSA with EVG was also confirmed by UV absorption spectra. The average distance,r, between donor (BSA) and acceptor (EVG) was obtained according to Förster’s theory of nonradiation energy transfer. Synchronous fluorescence and three-dimensional fluorescence spectra were used to investigate the conformational change of BSA molecules that occur upon addition of EVG and showed, upon binding, a possibility of increasing hydrophobicity around tryptophan residues of BSA.

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Більше джерел

Дисертації з теми "Intrinsic UV fluorescence"

Barulin, Aleksandr. "Label-free single protein fluorescence detection in the UV enhanced by aluminum plasmonic nanostructures." Thesis, Aix-Marseille, 2020. http://theses.univ-amu.fr.lama.univ-amu.fr/201204_BARULIN_360oitqab739occoku598wcb932u_TH.pdf.

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Les techniques de fluorescence de molécule individuelle permettent de suivre la dynamique moléculaire et les interactions dans les processus biologiques. Maintenant, la dynamique moléculaire des protéines est principalement accompagnée du marquage fluorescent externe. Cependant, une molécule attachée peut perturber la dynamique de protéines. Heureusement, la majorité des protéines contiennent le tryptophane ou la tyrosine qui absorbent et émettent la lumière dans le domaine spectral d'UV entre 260 nm et 400 nm. Ces acides aminés ont de basses efficacités quantiques, photostabilisées dans l'UV et la section efficace d'absorption, qui gênent la détection des protéines individuelles. Afin d'atteindre la sensitivité de l'auto fluorescence UV des protéines individuelles, nous développons un microscope confocal UV à la résolution temporelle avec les lasers de 266 nm et 295 nm. Nous quantifions la sensitivité de détection et l'effet des techniques de photostabilisation sur l'autofluorescence des protéines. La spectroscopie de corrélation de fluorescence (SCF) et les mesures de time-correlated single photon counting (TCSPC) fournissent des informations quantitatives du volume de détection, de l'amélioration de fluorescence (AF), et de la photokinétique accélérée des molécules émettant à la présence et à l'absence des nanostructures d'aluminium (Al). En utilisant le p-terphenyl, nous optimisons les nanostructures plasmoniques d'Al afin d'améliorer la fluorescence. Sous certaines conditions, le confinement de la lumière et l'AF dans les structures d'Al permettent d'appliquer la plasmonique UV pour la détection des protéines individuelles de beta-galactosidase sans marquage
Single molecule fluorescence techniques enable to monitor the molecular dynamics and interactions in the biological processes. Nowadays, the molecular dynamics of proteins is principally accompanied by external fluorescent labeling. However, an attached molecule might perturb the protein dynamics. Fortunately, a vast majority of proteins contain tryptophan and tyrosine that absorb and emit light in the UV range of 260-400 nm. These intrinsically fluorescent amino acids yield limited absorption cross-section, quantum yield, and photostability in the UV range, which hampers single protein UV autofluorescence detection. In order to reach single molecule sensitivity of protein UV autofluorescence, we develop a time-resolved UV confocal microscope with 266 nm and 295 nm excitations and the detection optics in the near UV. Based on the total fluorescence time traces, we quantify the single molecule sensitivity, the effect of photostabilization techniques on the protein autofluorescence. Fluorescence correlation spectroscopy (FCS) and time-correlated single photon counting (TCSPC) measurements provide quantitative information on the detection volume, the fluorescence enhancement factors, and the accelerated photokinetics of the UV emitting molecules in the presence and absence of the aluminum (Al) nanostructures. Using p-terphenyl as a bright UV emitting molecule, we optimize the Al plasmonic nanostructures to enhance the single molecule fluorescence. Under certain conditions, the light confinement and fluorescence enhancement in the aluminum nanostructures enable to apply the UV plasmonics for the single molecule detection of label-free beta-galactosidase protein

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Частини книг з теми "Intrinsic UV fluorescence"

Ernst, Oliver P., Christoph Bieri, Horst Vogel, and Klaus Peter Hofmann. "[32] Intrinsic biophysical monitors of transducin activation: Fluorescence, UV-visible spectroscopy, light scattering, and evanescent field techniques." In Methods in Enzymology, 471–89. Elsevier, 2000. http://dx.doi.org/10.1016/s0076-6879(00)15862-8.

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Тези доповідей конференцій з теми "Intrinsic UV fluorescence"

Yapoudjian, S., M. Ivanova, Olivier P. Uteza, Vladimir I. Marine, and Marc L. Sentis. "Surface intrinsic fluorescence spectroscopy of proteins using a UV linearly polarized pulsed laser beam." In International Conference on Atomic and Molecular Pulsed Lasers III, edited by Victor F. Tarasenko, Georgy V. Mayer, and Gueorgii G. Petrash. SPIE, 2000. http://dx.doi.org/10.1117/12.383460.

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Aparicio-Hernandez, Axel, Mariana Alfaro-Gomez, and Enoch Gutierrez-Herrera. "Stitching technique applied to UV intrinsic fluorescence imaging in an in vivo wound-healing model." In Infrared Remote Sensing and Instrumentation XXIX, edited by Marija Strojnik. SPIE, 2021. http://dx.doi.org/10.1117/12.2596638.

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Lewis, William, Juan-Pablo Padilla-Martinez, Antonio Ortega-Martinez, and Walfre Franco. "Preliminary investigation of intrinsic UV fluorescence spectroscopic changes associated with proteolytic digestion of bovine articular cartilage." In SPIE BiOS, edited by Robert R. Alfano and Stavros G. Demos. SPIE, 2016. http://dx.doi.org/10.1117/12.2212932.

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