Дисертації з теми "Interactions physicochimiques"
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Lecomte, Sophie. "Approches physicochimiques des differentes interactions impliquees dans l'activite des fluoroquinolones." Paris 6, 1995. http://www.theses.fr/1995PA066649.
Повний текст джерелаBelzunces, Luc P. "L'acétylcholinesterase d'un invertébré, Apis mellifera : propriétés physicochimiques, polymorphisme et interactions membranaires." Aix-Marseille 2, 1989. http://www.theses.fr/1989AIX22050.
Повний текст джерелаPinguet, Jean-Marc. "Méthodes de caractérisation des interactions physicochimiques entre un principe actif et les phospholipides." Paris 5, 1997. http://www.theses.fr/1997PA05P135.
Повний текст джерелаBoitte-Haddou, Nezha. "Synthèse de dérivés anthraquinoniques : étude de leurs propriétés physicochimiques, biologiques et d'interaction avec l'ADN." Lille 1, 1996. https://pepite-depot.univ-lille.fr/LIBRE/Th_Num/1996/50376-1996-91.pdf.
Повний текст джерелаZeyons, Ophélie. "Etudes des interactions physicochimiques et biologiques entre des nanoparticules manufacturées et des bactéries de l'environnement." Phd thesis, Université Pierre et Marie Curie - Paris VI, 2008. http://tel.archives-ouvertes.fr/tel-00331931.
Повний текст джерелаLa complexité de l'étude des nanoparticules nécessite une approche différente (multidisciplinaire) de celle des tests de toxicité utilisés pour les composés classiques. En effet, nous montrons que les paramètres physicochimiques (stabilité, agrégation, dissolution et état de surface) des nanoparticules dans le milieu de contact, influencent fortement la toxicité observée sur les cellules. De plus, les interactions physicochimiques (floculation, adsorption, mécanismes redox) sont liées au modèle biologique, en particulier à la présence d'exopolysaccharides (chez Synechocystis) comme barrière naturelle entre la paroi cellulaire et les nanoparticules. La composition du milieu de dispersion des nanoparticules (notamment son pH) a aussi une influence majeure sur la toxicité (survie et intégrité membranaire). Tandis que pour E. coli, ce sont majoritairement les nanoparticules qui provoquent la mortalité (confirmé par analyses métabonomiques par RMN).
Haudrechy, Pascale. "Caractérisation des interactions physicochimiques entre les surfaces de verre et vitrocéramique et l'alpha-lactalbumine en solution." Lille 1, 1990. http://www.theses.fr/1990LIL10003.
Повний текст джерелаKasselouri, Athéna. "Etude des proprietes physicochimiques de cyclodextrines amphiphiles en monocouches. Interactions avec les phospholipides et les polysaccharides." Paris 6, 1995. http://www.theses.fr/1995PA066124.
Повний текст джерелаParrello, Damien. "Conception de biosenseurs fluorescents multicolores pour l'identification in vivo des interactions bio-physicochimiques dans les systèmes minéral-bactérie." Thesis, Université de Lorraine, 2014. http://www.theses.fr/2014LORR0362/document.
Повний текст джерелаMonitoring terrestrial ecosystems requires a better understanding of the interactions between microorganisms, minerals and metals in the environment. To assess in vivo availability of metals such as iron in bacteria-mineral system, an approach based on whole-cell fluorescent biosensors and non-invasive spectroscopy was explored. This work led to the construction in Pseudomonas aeruginosa of a set of gene fusions coupling iron-regulated promoters to multicolour fluorescent reporters. The recombinant strains were used as sensors of structural iron availability in nontronites NAu-1 and NAu-2. The response of these biosensors was studied by coupling synchronous fluorescence spectroscopy (SFS) with canonical polyadic Candecomp/Parafac (CP) decomposition. On the basis of experimental designs favouring response diversity, the coupled SFS-CP method guarantees a joint estimate of gene expression from multiple promoters, even in highly fluorescent media. This novel method can solve the issue of spectral bleed-through of fluorescent proteins and provides a means to integrate multiple signals from combinations of whole-cell fluorescent bioreporters. In addition, we could show using SFS-CP that P. aeruginosa indirectly mobilize Fe(III) from nontronites primarily through the production of pyoverdine siderophore. The structural Fe(III) present on the edges of NAu-2 rather than NAu-1 particles appears to be more bioaccessible, suggesting that the distribution of Fe, in the tetrahedron and/or in the octahedron sites, governs the solubilization process
Pannecoucke, Xavier. "Synthese de phosphodiesters mixtes oxysterol-sucre, une premiere approche pour un ciblage tissulaire. Etudes physicochimiques des interactions oxysterol-membrane." Université Louis Pasteur (Strasbourg) (1971-2008), 1993. http://www.theses.fr/1993STR13166.
Повний текст джерелаCambier, Philippe. "Organisation des constituants et interactions physicochimiques au sein des microagrégats de sols. Application au système ferrallitique kaolinite-oxyde de fer." Paris 6, 1986. http://www.theses.fr/1986PA066359.
Повний текст джерелаCambier, Philippe. "Organisation des constituants et interactions physicochimiques au sein des microagrégats de sols application au système ferrallitique kaolinite-oxyde de fer." Grenoble 2 : ANRT, 1986. http://catalogue.bnf.fr/ark:/12148/cb375962273.
Повний текст джерелаRubio, Alexandre. "Dépollution d'un effluent industriel complexe par filtration membranaire : propriétés du latex naturel de skim concentré." Electronic Thesis or Diss., Montpellier, SupAgro, 2016. http://www.theses.fr/2016NSAM0013.
Повний текст джерелаNatural rubber latex (LCN) is an aqueous colloidal dispersion of polyisoprene (PI) natural rubber. After concentration, LCN (1,2 Mt/an) has many applications (surgical gloves, condoms) thanks to the properties of its main component, PI. Centrifugation concentration is mainly performed, producing a co-product (skim) with low rubber particle content (5% weight) but rich in non-rubber compounds. Its current recovery (H2SO4 coagulation) leads to low-quality rubber and the final effluent is hazardous for rivers (H2S). Previous filtration tests have proven the feasibility of the concept, but showed low productivity, coagulation problems and clogging.The thesis aims for a skim concentration process by ultrafiltration (UF), recovering the valuable PI and reducing the load of the effluent - for industrial application – within the Filtexcol project (ADEME). The process optimization included chemical and physicochemical characterization beyond only hydrodynamic parameters with a pilot plant. The chemical composition (non-rubber compounds) of the studied skims varies (lipid content, protein, anionic and nonionic surfactants (TA)). Their shear stability is measured with a rapid test developed to be factory use compatible, adapted to low load medium (PI ~3-5% ; non-PI ~2%). The UF suitability of the skim can be estimated via its high shear stability, the main limiting factor. The stability increases with the extractible protein and the non-ionic TA content. It is improved by precise additive amounts otherwise leading to the opposite effect. However stability is not related to the Zeta potential. The effect of nonionic TA (mono and diacylglycerol) formed by lipid hydrolysis indicates stabilization by steric repulsion, but the correlation also observed with proteins combined to anionic TA (soaps) in high concentration and optimal pH above 9,5 can also involve electrostatic repulsion.Our results show a complex structure different from the concentrated LCN’s. These results related to physcochemistry controlling the stability of the skim, combined with granulometry, allowed to choose through a 10L pilot the pump type (critical equipment due to the risk of high shear) and the membrane (ZrO2), to build a 100L pilot with a multicanal industrial membrane module, to determine a critical UF area (transmembrane pressure, tangential speed, retentate return rate) regarding the permeate flux J (critical flux, limit flux) and the partially optimized conditions via an experimental plan combined with artificial neural network simulation.PH control during cleaning and membrane conditioning ensures high J since early UF operation. But even outside of the critical area, initial J is very inferior to J-water (1/20), the rapid skim-membrane interaction introduces the main hydraulic resistance then J remains stable, reflecting the absence of subsequent interactions. Concentrating PI (retentate) increases proportionally the resistance to the volume concentration factor ~3 after which it decreases only slightly.J stabilizes at 20 L/h.m² after 20h of UF. The filtration state searched by the power law is SBM : low membrane fouling during concentration (partial blockage inside the pores). The entire PI and the particle-associated non-PIs (proteins and lipids) are retained and concentrated into the 100L pilot until ~40% dry matter; the chemical oxygen demand of the new effluent is divided by 4. These performance open the way to larger-scale trials and promising applications
LAMIOT, ERIC. "Influence de l'esterification de la beta-lactoglobuline et de la ceseine beta sur leurs caracteristiques physicochimiques et structurales et sur leurs interactions." Nantes, 1994. http://www.theses.fr/1994NANT2059.
Повний текст джерелаChouet, Agathe. "Fabrication des tuiles apéritives : étude des interactions formulation – structure – propriétés physico-chimiques en lien avec le procédé." Thesis, Nantes, Ecole nationale vétérinaire, 2019. http://www.theses.fr/2019ONIR124F.
Повний текст джерелаRestructured chips are made from a fried sheeted dough whose characteristics largely depend on the variability of the dehydrated potato which is the main raw material. In order to overcome these variations, it is essential to understand the role of the ingredients on the structure development during frying and on the resulting physicochemical and organoleptic properties. The role of added starch in the product was studied and highlighted the link between added starch and the dough aspect, physicochemical characteristics and structure of the final product. Native starch promotes an expanded, smooth, porous, brittle product with a lower fat content.The evolution of the product during frying process was studied: the physicochemical and structural characteristics of the product were found to be highly dependent on the glass transition.A frying time was determined for which the glass transition temperature was the same as the ambient temperature. For this frying time, the textural and structural properties acquired by the product during the cooling step change drastically. This work was carried out on a pilot equipment specially designed for this purpose and then adapted at an industrial scale to validate previous conclusions and provide information for the control of production lines. It does highlight the importance of the formulation in the manufacture and the product quality as well as the importance of the frying parameters in the chemical (water and fat content), physical (texture, global aspect), and structural properties (expansion, porosity) of the final product
Makayssi, Abdelatif. "Proprietes physicochimiques de melanges de composes tensioactifs cationiques a interactions faibles : relation entre la structure des systemes et la thermodynamique de la solubilisation micellaire." Paris 6, 1993. http://www.theses.fr/1993PA066166.
Повний текст джерелаBerredjem, Ali. "Contribution à l'étude de l'isocitrate déshydrogénase à NADP chez le champignon ectomycorhizien Laccaria bicolor. Purification, propriétés physicochimiques et interactions avec les enzymes du métabolisme azoté." Nancy 1, 1997. http://www.theses.fr/1997NAN10253.
Повний текст джерелаBazile, Vincent. "Diversité des stratégies de nutrition chez les plantes à urnes du genre Nepenthes : le rôle du fluide digestif, de ses propriétés physicochimiques et biotiques." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTS290.
Повний текст джерелаThe Nepenthes carnivorous plants genus encompasses about 160 species growing mostly in Southeast Asia in habitats characterised by their scarcity in absorbable nutrients. The leaf apex is modified into a tendril bearing a pitcher trap which allows the plant catching its prey and taking up the nutrients indispensable for its growth, mainly nitrogen. Reputed to be insectivorous, bearing traps equipped with a slippery wax covering the inner pitcher wall and with an enzymatic liquid involved in the digestion, Nepenthes species actually have a more diverse diet. Coprophagous, detritivorous, and insect-guild specialised species have been reported. These plants grow in more or less open environments, on sandy or peaty soils. Nepenthes traps show an inter-specific diversity of functional traits, bearing or not an attractive collar, a slippery waxy zone, and a viscoelastic liquid, which shelters a species-specific living infauna. Such different traits may reflect as many adaptations attesting to a diversity of nitrogen-sequestration. Many studies have focused on the role of slippery walls in insect capture but few of them have investigated the importance of the digestive fluid in both capture and digestion. Do the quantity of nitrogen available in the environment and the form of its availability influence the nitrogen foliar concentration of these plants, the source of their nitrogen supply and their carnivorous habit? How does the fluid influence prey capture and digestion? Can it contribute to the nitrogen-sequestration strategy of the plant? Do the fluids differ in their acidity, viscoelasticity, enzymatic pool, and in the abundance and species diversity of their infauna? What are the consequences of all these differences in terms of nitrogen recycling for the host plant?This PhD thesis explores the contribution of the digestive fluid and its physico-chemical and biotic properties on prey capture and digestion in 7 Nepenthes species in Brunei (Borneo).The first part shows that Nepenthes species have adapted to habitat differing in edaphic and biotic nitrogen available through distinct strategies of nitrogen acquisition. Pitcher contents’ analyses show that plants vary for their degree of insectivory. The variety of functional traits could explain the differences in their capture efficiency and prey diversity. While extrafloral nectar, slippery epicuticular wax and acidic fluid pH are associated with ants’ capture, cylinder-shaped traps, glandular trichomes and ant-association increase termites’ capture. Yellow colour, acidic pH and fluid viscoelasticity but above all, pitcher conicity, wide aperture diameter and magnitude of floral odour account for the abundance of flying insects. These Nepenthes species also strongly vary in their enzymatic secretions and inquilines spectra.In a second part, we show in situ how such differences in physico-chemical and biotic properties of the fluid partly account for the differences in nitrogen-sequestration strategy in these plants. Fluid pH and viscoelasticity influence the quantity and the nature of prey. Those physico-chemical properties also condition the richness of the aquatic ecosystem associated to the fluid, with an aquatic macrofauna more abundant and diverse in pitchers with lowly-acidic liquids and wide pitcher-openings. This inquiline macrofauna plays an essential role in prey degradation, and the presence of a top-predator is crucial for the nitrogen recycling to the plant.We finally discuss the major contribution of the fluid in the plant’s diet and its role in the probable adaptive radiation of the Nepenthes genus and conclude by a survey of the various forms of carnivory in these plants and their digestive systems, which range from an autonomous aggressive strategy to a mutualistic strategy, thus redefining the concept of carnivory in the plant’s world
Gassara, Dorra. "Simulation de la formation d'un dépôt de particules en microfiltration : effets des conditions hydrodynamiques et des interactions physicochimiques sur la morphologie et la perméabilité apparente du dépôt." Toulouse, INPT, 2007. http://ethesis.inp-toulouse.fr/archive/00000609/.
Повний текст джерелаThe formation of particles cake on the surface of the filter constitues one of the limiting phenomena of all the microfiltration operations. Progressively with its growth, this deposit, which adds an additional resitance to the fluid flow, reduced gradually the energetic efficiency of the filter system. The purpose of this work, is to characterize the influence of the hydrodynamic and physicochemical conditions on the morphology and the transport properties of the cake. Our study is carried out on a field made up of one or more unit elements representative of a filter surface models, provided with boundary periodic conditions. Moreover, in a first approaches quasi two-dimensional, the field is restricts with a Hele-shaw cell of which the thickness is equal to the particle diameter, so that the model of Darcy applies to the totality of the field. The particles are injected one by one starting from an initial position by chance selected and describe trajectories which take account of the structure of the flow and the physicochemical interactions of type DLVO with porous surface and the particles already deposited. The flow of Darcy is recomputed once the particle deposited
Gassara, Dorra Schmitz Philippe Ayadi Adbel-moneim. "Simulation de la formation d'un dépôt de particules en microfiltration effets des conditions hydrodynamiques et des interactions physicochimiques sur la morphologie et la perméabilité apparente du dépôt /." Toulouse : INP Toulouse, 2008. http://ethesis.inp-toulouse.fr/archive/00000609.
Повний текст джерелаThèse soutenue en co-tutelle. Titre provenant de l'écran-titre. Bibliogr. 106 réf.
Husson, Sophie. "Étude physicochimique et mécanique des interactions ciment-fillers. Application aux mortiers." Phd thesis, Ecole Nationale Supérieure des Mines de Saint-Etienne, 1991. http://tel.archives-ouvertes.fr/tel-00844622.
Повний текст джерелаHusson, Sophie. "Etude physicochimique et mécanique des interactions ciment-fillers : Application aux mortiers." Grenoble INPG, 1991. http://tel.archives-ouvertes.fr/tel-00844622.
Повний текст джерелаLe, Page Jean-François. "Interaction entre microenvironnement gazeux et évolution physicochimique des fromages au cours de l'affinage." Clermont-Ferrand 2, 2009. http://www.theses.fr/2009CLF21919.
Повний текст джерелаSCHWALLER, MARC ANTOINE. "Interaction de derives de l'ellipticine avec differentes macromolecules d'interet biologique : etude physicochimique et biologique." Paris 6, 1989. http://www.theses.fr/1989PA066451.
Повний текст джерелаRauwel, Gaétan. "Etude physicochimique des interactions entre tensioactifs non ioniques et ammonium quaternaires bicaténaires : conséquences sur l’activité biocide." Thesis, Lille 1, 2011. http://www.theses.fr/2011LIL10197.
Повний текст джерелаThe pre-disinfection treatment prior to the sterilization of medical devices (MD) in health care institutions, requires the use of combining both the properties of cleaning and disinfection. The active antimicrobial used mostly in pre-disinfectant cleaners is didecyldimethyl ammonium chloride ([DiC10] [Cl]), quaternary ammonium double-stranded. He is known particularly effective vis-à-vis micro-organisms, including yeasts such as Candida albicans. An assumption in chemistry formulation sets a mixture composed of the archetypal [DiC10] [Cl] and a nonionic polyethoxylated fatty alcohol type (CiEj), is sufficient to meet the dual demands of cleaning and disinfection. However, we have recently identified a significant loss of activity yeasticidal (Candida albicans) of [DiC10] [Cl] in the presence of certain types of CiEj, especially C12E8. This loss of activity is unacceptable to the treatment of DM. Since the mechanism is unclear biocide for double-stranded quaternary ammonium salts, particularly with respect to the yeast, we initially referred to a detailed study of structure-activity relationships. We have studied the influence of the length of the alkyl chain of quaternary ammonium and double-stranded nature of the ion-cons. The use of mathematical models has enabled us to establish a parabolic relationship between the biocidal activity of [DiC10] [Cl] and logP. In a second step, we focused on systems based on mixtures [DiC10] [Cl] and alkyl polyoxyethylene ether, CiEj (i = 10, 12, j = 4, 6, 8), various reports [ DiC10] [Cl] / CiEj. We studied the properties of self-aggregation and biocidal activity of Candida albicans mixtures. The combination of different experimental techniques (tensiometry, conductivity and specific quaternary ammonium electrode) and theoretical calculations led us to streamline the comicellisation. We have shown for the first time, how a better understanding of the behavior of mixtures to understand, or even to predict its biocidal activity. We have shown that the active form of [DiC10] [Cl] was the form unimère ([DiC10] [Cl] free) and the amount of [DiC10] [Cl] free could be optimized by adjusting the chemical structure of CiEj (C10E8 C12E8 versus). Convinced of the interest in eco-design of surfactants of vegetable origin, including the alkypolyglucosides (APG), we finally sought to replace all or part of polyethoxylated fatty alcohols. Anxious to maintain the biocidal activity of [DiC10] [Cl], we characterized the physicochemical and microbiological plans binary mixtures [DiC10] [Cl] / APG and ternary [DiC10] [Cl] / APG / CiEj. The latter case allowed us to consider combining biocidal efficacy, detergents and increased environmental benefit
Demortain, Geneviève. "Etude physicochimique de composés amorphes obtenus par hypertrempe : interactions magnétiques dans les polytypes hexagonaux de la perovskite." Bordeaux 1, 1987. http://www.theses.fr/1986BOR10652.
Повний текст джерелаDemortain, Geneviève. "Etude physicochimique de composés amorphes obtenus par hypertrempe interactions magnétiques dans les polytypes hexagonaux de la perovskite /." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb37604382b.
Повний текст джерелаDemortain, Geneviève. "Étude physicochimique de composés amorphes obtenus par hypertrempe : interactions magnétiques dans les polytypes hexagonaux de la perovskite." Bordeaux 1, 1987. http://www.theses.fr/1987BOR10657.
Повний текст джерелаGiraud-Panis, Marie-Josèphe. "Etude physicochimique des interactions entre la proteine receptrice de l'amp cyclique d'escherichia coli (crp) et les acides nucleiques." Orléans, 1990. http://www.theses.fr/1990ORLE2028.
Повний текст джерелаRichard, Jérôme Pascal Marie. "Interaction d'un brouillard d'eau avec un feu de nappe à l'échelle laboratoire : effets physicochimiques et influence sur le rayonnement." Poitiers, 2001. http://www.theses.fr/2001POIT2333.
Повний текст джерелаThe water mist is first characterized without flame : measurements of droplets diameters, droplets density and mass flow rate are performed. Then, the temperature field within the flame is established, without and then with water mist. Absorption fields and diffusion coefficients for two wavelengths are also determined in order to determine the relative contribution of absorption and diffusion processes. The knowledge of these experimental fields allows for the calculation of radiative flux towards the fuel surface with a Discrete Ordinates Method algorithm. Computed radiative flux values are compared with experimental values. Tomographic images of the mist-flame interaction flow are obtained. A PIV treatment of these images allows for the determination of droplets speed fields in order to get a better understanding of the dynamic interaction between the droplets and the flame. The study also shows that the introduction of steam within the flame has a chemical effect
Vandevelde, Margez Marie-Colette. "Contribution à l'étude physicochimique d'un polyélectrolyte naturel : la gomme arabique exsudat d'acacia Sénégal." Rouen, 1986. http://www.theses.fr/1986ROUES007.
Повний текст джерелаCornille, Fabrice. "Etude des interactions entre peptides et molécules de classe I du complexe majeur d'histocompatibilité, synthèse et étude physicochimique de la protéine de nucléocapside NCp10 du rétrovirus MoMuLU." Paris 5, 1991. http://www.theses.fr/1991PA05P604.
Повний текст джерелаRaffin, Fernanda Nervo. "Investigations physicochimiques de la perméabilité aux ions hydrogènes de films isolés d'acétophtalate de cellulose. Interaction des facteurs de formulation. Application à l'enrobage gastrorésistant de comprimés d'enzyme acido labile." Montpellier 1, 1995. http://www.theses.fr/1995MON13501.
Повний текст джерелаHadef, Redjein. "Mesure du nombre de markstein pour une flamme de premelange plissee." Aix-Marseille 1, 1987. http://www.theses.fr/1987AIX11127.
Повний текст джерелаLapp, Alain. "Synthese, conformation et interactions de molecules lineaires et ramifiees de polydimethylsiloxane : mise en evidence de lois d'universalite." Université Louis Pasteur (Strasbourg) (1971-2008), 1987. http://www.theses.fr/1987STR13108.
Повний текст джерелаGolinelli-Cohen, Marie-Pierre. "Ingénierie moléculaire de la ferredoxine(2Fe-2S) de Clostridium pasteurianum." Université Joseph Fourier (Grenoble ; 1971-2015), 1997. http://www.theses.fr/1997GRE10081.
Повний текст джерелаMary, Nathalie. "Caractérisations physico-chimique et biologique des cours d'eau de la Nouvelle-Calédonie. Proposition d'un indice biotique fondé sur l'étude des macroinvertébrés benthiques." Pacifique, 1999. http://portail-documentaire.univ-nc.nc/files/public/bu/theses_unc/These1999NathalieMary.pdf.
Повний текст джерелаQuadri, Mara Gabriela Novy. "Transferts de solutés dans les sols saturés et non saturés : application au pentachlorophénol." Grenoble 1, 1993. http://www.theses.fr/1993GRE10120.
Повний текст джерелаBanquy, Xavier. "Etude des propriétés physicochimiques des vecteurs nanoparticulaires." Thèse, 2009. http://hdl.handle.net/1866/3241.
Повний текст джерелаThis thesis reports the study of physical chemical properties of polymeric nanoparticles and their impact on the interaction with living cells. In particular we endeavoured to study the effect of the adhesive and mechanical properties of the vector on its capacity of penetration of the cellular membrane. With this intention, we firstly used nanoparticules of polylactic acid (PLA) functionalized on their surfaces with a ligand of the selectines E and P receptor. The grafting of the ligand on the particle’s surface was carried out thanks to a new experimental method guaranteeing the presence of the active molecule on the surface of the particle during its whole life cycle. This method consists in mixing a polymer functionalized with the ligand with another polymer not functionalized. The presence of the ligand on the surface of the nanoparticules formed starting from this mixture of polymers was confirmed by ToF SIMS analysis. We could show that the particles having the ligand grafted on their surface exhibit a higher adhesive capacity than their non-functionalized counterpart on endothelial cells HUVEC activated by various drugs. Nanoparticles adhesion on cells membrane was modulated by the level of expression of the receptors selectine E and P and also by the quantity of free ligand. These results show clearly that the functionalized particles possess all the characteristics of a pharmaceutical vector capable of targeting a particular receptor on a cell surface. The interaction between nanoparticules and cellular membrane can also be controlled by the mechanical properties of the cell as well as of the nanoparticule. To demonstrate it we have measured the elasticity of macrophagic cells deposited on various substrates using the SFA. We have thus showed that it was possible to control the cell mechanical properties at will by controlling the interaction between the cell and the substrate on which it rests. An increase of the cell elasticity is accompanied by an increase of the internalization of non-functionalized PLA nanoparticules. This suggests a major role of cytocortical mechanical properties in the capture of hard PLA particles. iv Lastly, we studied the effect of the mechanical properties of the nanoparticules on their cellular penetration capacity. With this intention, we synthesized hydrogel particles whose elasticity was controlled by the degree of crosslinking agent included in their formulation. The control of the mechanical properties of the nanoparticules was confirmed by the measurement of the Young modulus of the particles by AFM. The interaction of these particles with macrophagess showed that the mechanical properties of the particles affect various aspects related to the internalization of the nanoparticles. The internalization kinetics, the quantity of internalized particles and the mechanism of internalization depend all on the Young modulus of the nanoparticules. No differences in the intracellular pathway could be observed in spite of the fact that various pathways of internalization were observed for these nanoparticules. This last result can be explained by the fact that the nanoparticules are internalized by several mechanisms of simultaneously which facilitates their accumulation in intracellular digestive organelles. A simple model explaining these results is proposed and discussed.
Hammami, Gassara Dorra. "Simulation de la formation d'un dépôt de particules en microfiltration. Effets des conditions hydrodynamiques et des interactions physicochimiques sur la morphologie et la perméabilité apparente du dépôt." Phd thesis, 2007. http://oatao.univ-toulouse.fr/7677/1/hammami.pdf.
Повний текст джерела