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1
Fürst, Peter, Karin Pogan, Monika Hummel, Birgit Herzog, and Peter Stehle. "Reappraisal of Indispensable Amino Acids." Annals of Nutrition and Metabolism 41, no. 1 (1997): 1–9. http://dx.doi.org/10.1159/000177972.
2
Reeds, Peter J. "Dispensable and Indispensable Amino Acids for Humans." Journal of Nutrition 130, no. 7 (July 1, 2000): 1835S—1840S. http://dx.doi.org/10.1093/jn/130.7.1835s.
3
Laidlaw, S. A., and J. D. Kopple. "Newer concepts of the indispensable amino acids." American Journal of Clinical Nutrition 46, no. 4 (October 1, 1987): 593–605. http://dx.doi.org/10.1093/ajcn/46.4.593.
4
Hospodarenko, H. M. "CONTENT OF AMINO ACIDS IN WINTER WHEAT GRAIN DEPENDING ON THE VARIETY." Bulletin of Uman National University of Horticulture 2 (December 2020): 78–82. http://dx.doi.org/10.31395/2310-0478-2020-2-78-82.
Анотація:
The amino acid content of soft wheat grain was analyzed depending on the variety. It was found that the content of lysine, leucine, phenylalanine, tyrosine and cystine varies the most from the weather conditions of growing season. The total weight of amino acids varies from 14,01 to 17,46 % of grain depending on the variety of soft wheat. The share of indispensable amino acids is 27–29 % of their total mass. However, the sum content of indispensable amino acids varies significantly depending on the variety and line – from 3,81 to 5,10 %. The highest content of phenylalanine (0.55 %), lysine (0.51 %) and leucine (0.47 %) in the grain of soft wheat Panonicus (st) variety was found. The lowest methionine content is 0.1 %. The remaining amino acids ranged from 0,39 to 0,46 %. Among the dispensable amino acids, there is the highest content of glutamine (3,54 %) and proline (1.11 %), and the lowest content of cystine (0.1 %). The variation coefficient of the samples over the years of research was average for the amino acids serine, glutamine, glycine and arginine – low, histidine, asparagine and methionine – medium, and for the rest - high,. It is obvious that the content of amino acids also varies significantly depending on the weather conditions of the growing season of winter wheat. The content of indispensable amino acids in the grain of Kulundinka soft wheat varied from 0.16 to 0.95 %. The content of threonine (0,78 %) and leucine(0,95 %) was the highest, the content of methionine was the lowest – 0.16 %. The content of other indispensable amino acids ranged from 0,50 to 0,74 %. The content of dispensable amino acids ranged 0,22–4,03 %. The highest content was of proline (1,67 %) and glutamine (4,03 %). The content of other dispensable amino acids varied from 0,80 до 1,12 %. It was found that the content of basic amino acids in wheat grain of P 7 soft line varied from 0,09 to 3,92 %. Thus, the content of indispensable amino acids is dominated by threonine, lysine, valine and leucine (0,54–0,78 %). Among the indispensable are proline, asparagine, and glutamine (1,07–3,92 %). The lowest content among indispensable amino acids was characterized by methionine (0.09 %), among dispensable – cystine (0,27 %). The grain of Kulundinka wheat variety and P 7 line obtained by hybridization of Triticum aestivum L. / Triticum spelta L. has the highest number of indispensable amino acids, which are recommended to be used to obtain high-quality grain.
5
Jansone, Liene, and Daiga Kunkulberga. "Quantitative Assessment of Indispensable Amino Acids in the Flour Confectionery Food Products of Plant Origin — Tofu Cake and Tofu Muffin with Chickpea Flour." Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 77, no. 2 (April 1, 2023): 126–31. http://dx.doi.org/10.2478/prolas-2023-0018.
Анотація:
Abstract The article provides a study of the quantitative composition of amino acids in some baked flour confectionery product of plant and animal origin. The objective of the study was to determine the profiles of indispensable amino acids in several products. Source materials with high content of protein, and which have been used for the production of these confectionery products were studied. A source of protein in final products that contain ingredients of animal origin is cottage cheese, the content of which in the products amounts to 25% and 50%. The sources of protein in products that contain ingredients of plant origin include tofu, soya protein isolate, chickpea flour, and coconut milk. The results showed evidence of the presence of a full profile of indispensable amino acids in all tested products. The source ingredients had high biological value with well-balanced content of indispensable amino acids. The only exception was coconut milk, which had the lowest calculated amino acid amounts among the tested raw materials. Tofu cake contained leucine with the highest concentration — 0.78 g/100 g of product, followed by lysine (0.5 g/100 g). Tofu muffin with chickpea flour contained 0.63 g/100 g leucine and 0.45 g/100 g lysine. The profiles of indispensable amino acids showed that these products can excellently help to supplement daily needs for indispensable amino acids.
6
Jim, Susan, Vicky Jones, Stanley H. Ambrose, and Richard P. Evershed. "Quantifying dietary macronutrient sources of carbon for bone collagen biosynthesis using natural abundance stable carbon isotope analysis." British Journal of Nutrition 95, no. 6 (June 2006): 1055–62. http://dx.doi.org/10.1079/bjn20051685.
Анотація:
The diets of laboratory rats were isotopically and nutritionally manipulated using purifiedC3 and/or C4 macronutrients to investigate the routing of dietary carbonto bone collagen biosynthesis. Diets were formulated with purified proteins, carbohydrates andlipids of defined composition and natural abundance stable isotope ratios. Bulk protein and constituent amino acid δ13C values determined for whole diet and bone collagen provided the basis for assessing isotopic fractionation and estimating the degree of routing versus synthesis de novo of essential, non-essential and conditionally indispensable amino acids. Essential and conditionally indispensable amino acids were shown to be routed from diet to collagen with little isotopic fractionation whereas non-essential amino acids differed by up to 20‰. Mathematical modelling of the relationships between macronutrient and tissue δ13C values provided qualitative and quantitative insights into the metabolic and energetic controls on bone collagen biosynthesis. Essential amino acids comprise 21·7% of the carbon in collagen, defining the minimum amount of dietary carbon routing. Estimates of 42 and 28% routing were shown for the non-essential amino acids, glycine and aspartate, respectively. In total, the routing of non-essential and conditionally indispensable amino acids was estimated to equal 29·6% of the carbon in collagen. When the contribution of carbon from the essential amino acids is also considered, we arrive at an overall minimum estimate of 51·3% routing of dietary amino acid carbon into bone collagen.
7
Gietzen, Dorothy W. "Brain Signaling of Indispensable Amino Acid Deficiency." Journal of Clinical Medicine 11, no. 1 (December 30, 2021): 191. http://dx.doi.org/10.3390/jcm11010191.
Анотація:
Our health requires continual protein synthesis for maintaining and repairing tissues. For protein synthesis to function, all the essential (indispensable) amino acids (IAAs) must be available in the diet, along with those AAs that the cells can synthesize (the dispensable amino acids). Here we review studies that have shown the location of the detector for IAA deficiency in the brain, specifically for recognition of IAA deficient diets (IAAD diets) in the anterior piriform cortex (APC), with subsequent responses in downstream brain areas. The APC is highly excitable, which makes is uniquely suited to serve as an alarm for reductions in IAAs. With a balanced diet, these neurons are kept from over-excitation by GABAergic inhibitory neurons. Because several transporters and receptors on the GABAergic neurons have rapid turnover times, they rely on intact protein synthesis to function. When an IAA is missing, its unique tRNA cannot be charged. This activates the enzyme General Control Nonderepressible 2 (GCN2) that is important in the initiation phase of protein synthesis. Without the inhibitory control supplied by GABAergic neurons, excitation in the circuitry is free to signal an urgent alarm. Studies in rodents have shown rapid recognition of IAA deficiency by quick rejection of the IAAD diet.
8
Tharrey, Marion, Francois Mariotti, Andrew Mashchak, Pierre Barbillon, Maud Delattre, Jean-François Huneau, and Gary E. Fraser. "Patterns of amino acid intake are strongly associated with cardiovascular mortality, independently of the sources of protein." International Journal of Epidemiology 49, no. 1 (September 27, 2019): 312–21. http://dx.doi.org/10.1093/ije/dyz194.
Анотація:
Abstract Background The intake of specific amino acids (AA) has been associated with cardiovascular health, but amino acids are consumed together as dietary protein. Here we investigated the association between identified patterns of amino acid intake and cardiovascular mortality. Methods A total of 2216 cardiovascular deaths among 79 838 men and women from the Adventist Health Study-2 were included in our analysis. Baseline dietary patterns based on the participants' amino acids intakes were derived by factor analysis. Using Cox regression analyses, we estimated multivariate-adjusted hazard ratios (HRs) adjusted for sociodemographic and lifestyle factors and other dietary components. Results Three patterns of amino acids were identified. Factor 1 was positively associated with cardiovascular disease (CVD) mortality [hazard ratio (HR)Q5-Q1: 1.62, 98.75% confidence interval (CI): 1.15, 2.28; P-trend <0.001]; and Factors 2 and 3 were inversely associated with CVD mortality (HR Q5-Q1 Factor 2: 0.74, 98.75% CI: 0.53, 1.04; P-trend <0.01 and HR Q5-Q1 Factor 3: 0.65, 98.75% CI: 0.44, 0.95; P-trend <0.05]. The associations with Factor 1 (with high loadings on indispensable amino acids such as branched chain amino acids, lysine, methionine) and Factor 3 (with high loadings on non-indispensable amino acids, namely arginine, glycine, aspartate+asparagine) remained significant after further adjustment for nutrient intake and for the five protein source patterns identified previously (HR Q5-Q1: 1.56 (0.99, 2.45) and 0.55 (0.35, 0.85); P-trends < 0.01). Conclusions Indispensable AA have a positive and some non-indispensable AA have a negative, independent, strong association with the risk of cardiovascular mortality.
9
Cervantes-Pahm, Sarah K., Yanhong Liu, and Hans H. Stein. "Digestible indispensable amino acid score and digestible amino acids in eight cereal grains." British Journal of Nutrition 111, no. 9 (January 30, 2014): 1663–72. http://dx.doi.org/10.1017/s0007114513004273.
Анотація:
To determine values for the digestible indispensable amino acid score (DIAAS), it is recommended that ileal amino acid (AA) digestibility values obtained in growing pigs are used to characterise protein quality in different foods. Therefore, an experiment was conducted to determine the standardised ileal digestibility (SID) of AA in eight cereal grains (yellow dent maize, Nutridense maize, dehulled barley, dehulled oats, polished white rice, rye, sorghum and wheat) fed to pigs, where SID values in pigs can be used to calculate approximate DIAAS values in humans. In the present experiment, twenty-four barrows with a T-cannula inserted in the distal ileum were allotted to eight diets and fed for three periods to give a total of nine replicate pigs per diet. Each period lasted 14 d, and ileal digesta samples were collected on days 13 and 14. Among the SID values obtained for all cereal grains, values for total indispensable AA were greatest (P< 0·05) in rice and lowest (P< 0·05) in rye and sorghum. The concentrations of SID indispensable AA in rice were less (P< 0·05) than in dehulled oats, but greater (P< 0·05) than in the other cereal grains, and the concentrations of SID indispensable AA in Nutridense maize were greater (P< 0·05) than in yellow dent maize and sorghum, but less (P< 0·05) than in the other cereal grains, except rye. In conclusion, results indicate that to meet dietary requirements for AA in humans, diets based on yellow dent maize or sorghum require more AA supplementation than diets based on other cereal grains.
10
Henky Saputra, Dedyanto. "Suplemen Putih Telur dalam Tatalaksana Hipoalbuminemia." Cermin Dunia Kedokteran 48, no. 10 (October 1, 2021): 410–13. http://dx.doi.org/10.55175/cdk.v48i10.138.
Анотація:
Putih telur merupakan sumber protein dengan kualitas asam amino yang baik. Selain mengandung asam amino esensial, Digestible Indispensable Amino Acid Score dari putih telur juga tinggi. Berbagai penelitian (banyak dilakukan di Indonesia) menunjukkan bahwa suplemen putih telur bermanfaat memperbaiki kondisi hipoalbuminemia pasien rawat inap, hemodialisis, pasien TB paru, pre-eklamsia, dan luka bakar.
Egg-whites are a source of protein with good quality amino acids. Apart from containing essential amino acids, the Digestible Indispensable Amino Acid Score of egg whites is also high. Various studies (mostly conducted in Indonesia) have shown egg white supplements benefits in improving hypoalbuminemia in hospitalization, hemodialysis, pulmonary tuberculosis, pre-eclampsia, and burns.
11
Saputra, Dedyanto Henky. "Suplemen Putih Telur dalam Tatalaksana Hipoalbuminemia." Cermin Dunia Kedokteran 48, no. 10 (October 25, 2021): 410. http://dx.doi.org/10.55175/cdk.v48i10.1514.
Анотація:
<p>Putih telur merupakan sumber protein dengan kualitas asam amino yang baik. Selain mengandung asam amino esensial, Digestible Indispensable Amino Acid Score dari putih telur juga tinggi. Berbagai penelitian (banyak dilakukan di Indonesia) menunjukkan bahwa suplemen putih telur bermanfaat memperbaiki kondisi hipoalbuminemia pasien rawat inap, hemodialisis, pasien TB paru, pre-eklamsia dan luka bakar.</p><p>Egg-whites are a source of protein with good quality amino acids. Apart from containing essential amino acids, the Digestible Indispensable Amino Acid Score of egg whites is also high. Various studies (mostly conducted in Indonesia) have shown egg white supplements benefits in improving hypoalbuminemia in hospitalization, hemodialysis, pulmonary tuberculosis, pre-eclampsia and burns.</p>
12
Dasgupta, Mou, Joseph R. Sharkey, and Guoyao Wu. "Inadequate Intakes of Indispensable Amino Acids Among Homebound Older Adults." Journal of Nutrition For the Elderly 24, no. 3 (April 14, 2005): 85–99. http://dx.doi.org/10.1300/j052v24n03_07.
13
Christensen, Halvor N. "Mass-Action Effects Are Indispensable for All the Amino Acids." Nutrition Reviews 34, no. 1 (April 27, 2009): 30–31. http://dx.doi.org/10.1111/j.1753-4887.1976.tb05669.x.
14
Jansman, A. J. M., O. Cirot, E. Corrent, W. Lambert, J. Ensink, and J. Th M. van Diepen. "Interaction and imbalance between indispensable amino acids in young piglets." Animal 13, no. 5 (2019): 941–49. http://dx.doi.org/10.1017/s175173111800263x.
15
Ravindran, V., L. I. Hew, G. Ravindran, and W. L. Bryden. "Apparent ileal digestibility of amino acids in dietary ingredients for broiler chickens." Animal Science 81, no. 1 (August 2005): 85–97. http://dx.doi.org/10.1079/asc42240085.
Анотація:
AbstractThe apparent ileal digestibility coefficients of amino acids in 107 samples representing 22 food ingredients were determined using 6-week-old broiler chickens. The ingredients assayed included five cereals (barley, maize, sorghum, triticale and wheat), two cereal by-products (rice polishings and wheat middlings), four oilseed meals (canola, cottonseed, soya-bean and sunflower meals), full-fat canola, maize gluten meal, four grain legumes (chickpeas, faba beans, field peas and lupins) and five animal protein sources (blood, feather, fish, meat and meat and bone meals). The mean ileal digestibility coefficients of amino acids in wheat and maize were higher than those in sorghum, triticale and barley. However, variations observed in individual amino acid digestibilities among samples within cereal type were greater than those determined between cereals. Threonine and lysine were the least digestible indispensable amino acids in the five cereals evaluated. The most digestible indispensable amino acid was phenylalanine in wheat and, leucine in maize and sorghum. In the case of the wheat middlings and rice polishings, threonine was the least digestible indispensable amino acid and arginine was the best digested.In the oilseed meals assayed, amino acid digestibility was highest for soya-bean and sunflower meals, intermediate for canola meal and lowest for cottonseed meal. Ileal digestibility coefficients of amino acids in lupins were found to be slightly lower than those in soya-bean meal. The amino acid digestibilities of field peas, faba beans and chickpeas were considerably lower than those of lupins. Digestibility of arginine was the highest and that of threonine was the lowest of the indispensable amino acids in oilseed meals and grain legumes, except in cottonseed meal. Lysine was the least digestible amino acid in cottonseed meal.In the animal protein sources assayed, digestibility coefficients of amino acids in blood meal were high, intermediate in fish meal, and low in meat meal, meat and bone meal and feather meal. Variation in amino acid digestibility coefficients determined for blood meal samples was small. However, wide variations in amino acid digestibilities were observed for other animal protein sources, highlighting significant batch-to-batch differences. In particular, marked variations were determined for meat meal and meat and bone meal samples. Cystine was the least digested amino acid in animal protein meals, with the exception of blood meal in which isoleucine had the lowest digestibility. The limitations of using apparent digestibility values in diet formulations and the concept of the standardized digestibility system to overcome these limitations are discussed.
16
Elango, Rajavel, Crystal Levesque, Ronald O. Ball, and Paul B. Pencharz. "Available versus digestible amino acids – new stable isotope methods." British Journal of Nutrition 108, S2 (August 2012): S306—S314. http://dx.doi.org/10.1017/s0007114512002498.
Анотація:
The nutritive value of food protein sources is dependent on the amino acid composition and the bioavailability of the nutritionally indispensable amino acids. Traditionally the methods developed to determine amino acid bioavailability have focused on intestinal absorption or digestibility, which is calculated as the percent of amino acid intake that does not appear in digesta or faeces. Traditional digestibility based methods do not always account for gut endogenous amino acid losses or absorbed amino acids which are unavailable due to the effect of heat processing and the presence of anti-nutritional factors, though methods have been developed to address these issues. Furthermore, digestibility based methods require the use of animal models, thus there is a need to developin vivomethods that can be applied directly in human subjects to identify the proportion of dietary amino acids which is bioavailable, or metabolically available to the body for protein synthesis following digestion and absorption. The indicator amino acid oxidation (IAAO) method developed in our laboratory for humans has been systematically applied to determine almost all indispensable amino acid requirements in adult humans. Oxidation of the indicator amino acid is inversely proportional to whole body protein synthesis and responds rapidly to changes in the bioavailability of amino acids for metabolic processes. Using the IAAO concept, we developed a newin vivomethod in growing pigs, pregnant sows and adult humans to identify the metabolic availability of amino acids in foods. The stable isotope based metabolic availability method is suitable for rapid and routine analysis in humans, and can be used to integrate amino acid requirement data with dietary amino acid availability of foods.
17
Olga A. Gracheva, Alizade S. Gasanov, Damir R. Amirov, Bulat F. Tamimdarov, Dina M. Mukhutdinova, Sergey Yu. Smolentsev, Irina I. Strelnikova, and Tatyana V. Izekeeva. "Study of the effect of different levels of arginine in feed on broiler chickens." International Journal of Research in Pharmaceutical Sciences 11, no. 1 (January 28, 2020): 908–12. http://dx.doi.org/10.26452/ijrps.v11i1.1913.
Анотація:
Amino acids are the basic structural units of protein molecules in the body. Currently, about 300 amino acids are known, of which 26 are studied best. Amino acids or their derivatives (for example, immune bodies) are part of enzymes, hormones, pigments, and other specific substances that play a crucial role in digestive and metabolic processes. In the process of metabolism, many amino acids are synthesized in the body from other amino acids or compounds, and therefore they are called interchangeable. Amino acids that are not synthesized in the body or are formed in insufficient quantities are called indispensable. According to the content and ratio of essential amino acids, feed proteins are subdivided into full and inferior. Deficiency, absence, or imbalance of essential amino acids in animal diets is accompanied by a deterioration in protein use, metabolic disorders, and decreased productivity. The following amino acids are indispensable; arginine, viburnum, histidine, isoleucine, leucine, lysine, methionine, threonine, tryptophan, phenylalanine. Among the essential amino acids, especially important ones, are those called critical. These are lysine, methionine + cystia, threonine, and tryptophan. A deficiency, absence, or imbalance of essential amino acids in animal diets is accompanied by a deterioration in protein use, metabolic disorders, and decreased productivity. Research and production experiment was conducted within Olenka Poultry Factory LLC and the problematic research laboratory of feed additives of the Department of Animal Feeding and Feed Technology named after P. D. Pshenichny at the National University of Life and Environmental Sciences. The effective level of arginine in compound feed for broilers of the Cobb-500 cross was experimentally determined. The use of compound feed with arginine levels in the first rearing period (1 to 10 days) - 1.28%, in the second (11 to 22 days) - 1.15% and in the third (23 to 42 days) - 1.11% gives the opportunity to get broiler chickens at the age of 42 days, weighing 2.654 kg, at a feed expenditure of 1.78 kg per 1 kg of gain.
18
Torrallardona, David, and C. Ian Harris. "Preparative isolation of nine indispensable amino acids from a single sample." Journal of Chromatography A 778, no. 1-2 (August 1997): 383–88. http://dx.doi.org/10.1016/s0021-9673(97)00326-9.
19
Pakhomov, V. I., S. V. Braginets, O. N. Bakhchevnikov, A. S. Alferov, A. I. Rukhlyada, and A. S. Babajanyan. "The results of experimental studies of extrusion of feed containing wheat grain and black soldier fly larvae biomass." Agricultural Science Euro-North-East 21, no. 1 (March 4, 2020): 28–42. http://dx.doi.org/10.30766/2072-9081.2020.21.1.28-42.
Анотація:
The experimental studies were carried out in the Rostov region of Russia in 2019. Three variants of grinded wheat grain mixed with biomass of black soldier fly larvae with raw animal material content of 10, 12.5 and 15 % by mass were extruded at varying temperature. The content of amino acids in raw material and finished extrudate was determined. It has been established that the feed mixture containing grinded wheat grain and biomass of black soldier fly larvae can be successfully extruded at the temperature of 121-135 ºC. As the extrusion temperature rises in the range of 115º-140 ºС, the content of amino acids in a finished extrudate decreases. Change in larvae content in the feed mixture does not affect the nature of the correlation between the amino acids content in the extrudate and the extrusion temperature and process behavior. It has been found that with increasing temperature the amino acids content in the extruded feed decreases with an accelerated velocity irrespective of the insect biomass content. The rational temperature range for extrusion of feed mixture containing grinded wheat grain and larvae biomass was determined within 121-127 ºC. This range provides no more than 30 % decrease in indispensable amino acids content in the extrudate. The extruded feed containing 15% of insect larvae biomass contains 9.6±0.13 % of amino acids, including 4.38±2.01 % of indispensable amino acids. Extrusion of larvae biomass mixed with grain of cereal crops is considered to be a promising direction for improving production of feed for fish and farm animals.
20
Samadi, Samadi. "Konsep Ideal Protein (Asam amino) Fokus Pada Ternak Ayam Pedaging (review artikel)." Jurnal Agripet 12, no. 2 (October 1, 2012): 42–28. http://dx.doi.org/10.17969/agripet.v12i2.202.
Анотація:
Ideal protein (amino acids) concept focused on broiler ABSTRACT. Accurate estimation of the amino acid requirement is very importance for diet formulation in growing animals such as broiler. Inadequate formulation of dietary amino acids will impair protein utilization and increase the total nitrogen output to the environment. Amino acids requirement of animals changes due to dietary, sex, and genetic and environmental factors. To address all potential combinations of factors with dose-response experiments is almost impossible. Thus the ideal protein concept provides a simple and effective approach to solve this problem. Based on this concept, the requirement of indispensible amino acids can be determined. For this concept, lysine has been used as the reference amino acid to calculate other indispensable amino acids due to several considerations. This paper will review more detailed about protein concept based on amino acid profile by using broiler as sampel review.
21
Paul, Cristiana, Suzane Leser, and Steffen Oesser. "Significant Amounts of Functional Collagen Peptides Can Be Incorporated in the Diet While Maintaining Indispensable Amino Acid Balance." Nutrients 11, no. 5 (May 15, 2019): 1079. http://dx.doi.org/10.3390/nu11051079.
Анотація:
The results of twenty years of research indicate that the inclusion of collagen peptides in the diet can lead to various improvements in health. According to the current protein quality evaluation method PDCAAS (Protein Digestibility-corrected Amino Acid Score), collagen protein lacks one indispensable amino acid (tryptophan) and is therefore categorized as an incomplete protein source. Collagen protein displays a low indispensable amino acid profile, yet as a functional food, collagen is a source of physiologically active peptides and conditionally indispensable amino acids that have the potential to optimize health and address physiological needs posed by aging and exercise. The objective of this study was to determine the maximum level of dietary collagen peptides that can be incorporated in the Western pattern diet while maintaining its indispensable amino acid balance. Iterative PDCAAS calculations showed that a level as high as 36% of collagen peptides can be used as protein substitution in the daily diet while ensuring indispensable amino acid requirements are met. This study suggests that the effective amounts of functional collagen peptides (2.5 to 15 g per day) observed in the literature are below the maximum level of collagen that may be incorporated in the standard American diet.
22
Bröer, Stefan. "Amino Acid Transporters as Targets for Cancer Therapy: Why, Where, When, and How." International Journal of Molecular Sciences 21, no. 17 (August 26, 2020): 6156. http://dx.doi.org/10.3390/ijms21176156.
Анотація:
Amino acids are indispensable for the growth of cancer cells. This includes essential amino acids, the carbon skeleton of which cannot be synthesized, and conditionally essential amino acids, for which the metabolic demands exceed the capacity to synthesize them. Moreover, amino acids are important signaling molecules regulating metabolic pathways, protein translation, autophagy, defense against reactive oxygen species, and many other functions. Blocking uptake of amino acids into cancer cells is therefore a viable strategy to reduce growth. A number of studies have used genome-wide silencing or knock-out approaches, which cover all known amino acid transporters in a large variety of cancer cell lines. In this review, these studies are interrogated together with other databases to identify vulnerabilities with regard to amino acid transport. Several themes emerge, such as synthetic lethality, reduced redundancy, and selective vulnerability, which can be exploited to stop cancer cell growth.
23
Zou, Yupiao, Jianlin Han, Ashot S. Saghyan, Anna F. Mkrtchyan, Hiroyuki Konno, Hiroki Moriwaki, Kunisuke Izawa, and Vadim A. Soloshonok. "Asymmetric Synthesis of Tailor-Made Amino Acids Using Chiral Ni(II) Complexes of Schiff Bases. An Update of the Recent Literature." Molecules 25, no. 12 (June 12, 2020): 2739. http://dx.doi.org/10.3390/molecules25122739.
Анотація:
Tailor-made amino acids are indispensable structural components of modern medicinal chemistry and drug design. Consequently, stereo-controlled preparation of amino acids is the area of high research activity. Over last decade, application of Ni(II) complexes of Schiff bases derived from glycine and chiral tridentate ligands has emerged as a leading methodology for the synthesis of various structural types of amino acids. This review article summarizes examples of asymmetric synthesis of tailor-made α-amino acids via the corresponding Ni(II) complexes, reported in the literature over the last four years. A general overview of this methodology is provided, with the emphasis given to practicality, scalability, cost-structure and recyclability of the chiral tridentate ligands.
24
Zhumabay, Aikerim, Assiya Serikbayeva, Sabira Kozykan, Yus Aniza Yusof, and Aigul Kozhakhmetova. "Determination of the fatty and amino acid composition of camel milk, milk powder and shubat." Potravinarstvo Slovak Journal of Food Sciences 17 (November 7, 2023): 918–28. http://dx.doi.org/10.5219/1931.
Анотація:
Camel milk is considered an essential source of nutrition and an effective remedy with healing properties in treating several diseases. Shubat, a fermented drink made from camel milk, contains easily digestible proteins, determining its nutritional value. Meanwhile, few studies have analysed the fatty and amino acid composition of Bactrian camel milk, milk powder and shubat in Kazakhstan. In this paper, we used the gas chromatography-mass spectrometry method to determine milk the fatty and amino acid composition of Kazakhstan camel milk and camel milk powder and submit samples. As a result, significant differences in the fatty acid and amino acid compositions were observed among samples of raw milk, milk powder and shubat. differences were found in all amino acids. The most representative fatty acids in the three groups were С16:0, С18:0, С18:1n9c, С14:0 FAs. In camel milk samples, among indispensable amino acids, lysine (29.64%) was the highest in concentration, followed by methionine (25.68%). Some polyunsaturated fatty acids (PUFAs) such as С18:3n3c, С20:4n6, С18:3n3c, С20:3n3c 8,11,14 were found only in shubat samples. Furthermore, we revealed a significant decrease in both dispensable (DAA) and indispensable (IDAA) contents in camel milk powder. Meanwhile, an increase in the quantitative content of amino acids has been observed in shubat, especially in threonine (166.86%), aspargine (156.34%), alanine (114.48%), etc. The results provide a theoretical basis for additional studies of camel milk composition of Bactrian camel in Kazakhstan.
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Hasegawa, Hiroshi, Takehisa Matsukawa, Yoshihiko Shinohara, Ryuichi Konno, and Takao Hashimoto. "Role of renal d-amino-acid oxidase in pharmacokinetics of d-leucine." American Journal of Physiology-Endocrinology and Metabolism 287, no. 1 (July 2004): E160—E165. http://dx.doi.org/10.1152/ajpendo.00397.2003.
Анотація:
d-Amino acids are now recognized to be widely present in mammals. Renal d-amino-acid oxidase (DAO) is associated with conversion of d-amino acids to the corresponding α-keto acids, but its contribution in vivo is poorly understood because the α-keto acids and/or l-amino acids formed are indistinguishable from endogenous compounds. First, we examined whether DAO is indispensable for conversion of d-amino acids to their α-keto acids by using the stable isotope tracer technique. After a bolus intravenous administration of d-[2H7]leucine to mutant mice lacking DAO activity (ddY/DAO−) and normal mice (ddY/DAO+), elimination of d-[2H7]leucine and formation of α-[2H7]ketoisocaproic acid ([2H7]KIC) and l-[2H7]leucine in plasma were determined. The ddY/DAO− mice, in contrast to ddY/DAO+ mice, failed to convert d-[2H7]leucine to [2H7]KIC and l-[2H7]leucine. This result clearly revealed that DAO was indispensable for the process of chiral inversion of d-leucine. We further investigated the effect of renal mass reduction by partial nephrectomy on elimination of d-[2H7]leucine and formation of [2H7]KIC and l-[2H7]leucine. Renal mass reduction slowed down the elimination of d-[2H7]leucine. The fraction of conversion of d-[2H7]leucine to [2H7]KIC in sham-operated rats was 0.77, whereas that in five-sixths-nephrectomized rats was 0.25. The elimination behavior of d-[2H7]leucine observed in rats suggested that kidney was the principal organ responsible for converting d-leucine to KIC.
26
Iwagishi, Ryo, Rika Tanaka, Munenosuke Seto, Tomoyo Takagi, Naoko Norioka, Tomoe Ueyama, Teruhisa Kawamura, Junichi Takagi, Yoshihiro Ogawa, and Kyoko Shirakabe. "Negatively charged amino acids in the stalk region of membrane proteins reduce ectodomain shedding." Journal of Biological Chemistry 295, no. 35 (June 24, 2020): 12343–52. http://dx.doi.org/10.1074/jbc.ra120.013758.
Анотація:
Ectodomain shedding is a post-translational modification mechanism by which the entire extracellular domain of membrane proteins is liberated through juxtamembrane processing. Because shedding rapidly and irreversibly alters the characteristics of cells, this process is properly regulated. However, the molecular mechanisms governing the propensity of membrane proteins to shedding are largely unknown. Here, we present evidence that negatively charged amino acids within the stalk region, an unstructured juxtamembrane region at which shedding occurs, contribute to shedding susceptibility. We show that two activated leukocyte cell adhesion molecule (ALCAM) protein variants produced by alternative splicing have different susceptibilities to ADAM metallopeptidase domain 17 (ADAM17)-mediated shedding. Of note, the inclusion of a stalk region encoded by a 39-bp-long alternative exon conferred shedding resistance. We found that this alternative exon encodes a large proportion of negatively charged amino acids, which we demonstrate are indispensable for conferring the shedding resistance. We also show that the introduction of negatively charged amino acids into the stalk region of shedding-susceptible ALCAM variant protein attenuates its shedding. Furthermore, we observed that negatively charged amino acids residing in the stalk region of Erb-B2 receptor tyrosine kinase 4 (ERBB4) are indispensable for its shedding resistance. Collectively, our results indicate that negatively charged amino acids within the stalk region interfere with the shedding of multiple membrane proteins. We conclude that the composition of the stalk region determines the shedding susceptibility of membrane proteins.
27
Rahgo, Zahra, Hamid reza Samadlouie, Shideh Mojerlou, and Kambiz Jahanbin. "Statistical Optimization of Culture Conditions for Protein Production by a Newly Isolated Morchella fluvialis." BioMed Research International 2019 (December 24, 2019): 1–9. http://dx.doi.org/10.1155/2019/7326590.
Анотація:
Morchella fungi are considered a good source of protein. The ITS region was used to identify Morchella isolated in the northern region of Iran. The isolated fungus was very similar to Morchella fluvialis. M. fluvialis was first isolated in Iran. Dried biomass of M. fluvialis contained 9% lipids and 50% polysaccharides. Fatty acid profiles of lipids of M. fluvialis are mainly made up of linoleic acid (C18:2) (62%), followed by palmitic acid (C16:0) (12%). Testosterone (TS) was also detected (0.732 ng/dry weight biomass (DWB)) in the hormone profile of this new isolated species. Then, various protein and carbon sources as variable factors were applied to identify the key substrates, which stimulated protein production using the one-factor-at-a-time method. Key substrates (glucose and soybean) were statistically analyzed to determine the optimum content of the protein and DWB accumulation using response surface methods. The highest protein content (38% DWB) was obtained in the medium containing 80 g/l glucose and 40 g/l soybean powder. Total nutritionally indispensable amino acids and conditionally indispensable amino acids constitute 55.7% crude protein. That is to say, these adequate quantities of essential amino acids in the protein of M. fluvialis make it a good and promising source of essential amino acids for human diet.
28
Millward, D. J., and J. P. W. Rivers. "The need for indispensable amino acids: The concept of the anabolic drive." Diabetes / Metabolism Reviews 5, no. 2 (March 1989): 191–211. http://dx.doi.org/10.1002/dmr.5610050207.
29
Pezzali, Julia Guazzelli. "62 Sulphur Amino Acids: Assessing Requirements and Utilization in Dogs and Cats." Journal of Animal Science 101, Supplement_3 (November 6, 2023): 115–16. http://dx.doi.org/10.1093/jas/skad281.140.
Анотація:
Abstract Methionine (Met), cyst(e)ine (Cys), homocysteine and taurine are the four sulfur-containing amino acids. Met can serve as a substrate for protein synthesis or to produce homocysteine, which can be remethylated to Met or irreversibly catabolized to Cys. Unlike dogs, cats are unable to further metabolize Cys to taurine, making the latter an indispensable amino acid for the cat. Met, on the other hand, is a dietary indispensable amino acid for all mammals with Cys having the ability to spare part of the Met requirement (total sulfur amino acid requirement; Met + Cys). The amount of Met that cannot be replaced by Cys is defined as the Met requirement (NRC, 2006). Empirical determinations of the Met requirement in adult dogs and cats are still limited and the recommendations proposed by the NRC (2006) are based on studies that used insensitive techniques for mature animals. When a more robust technique, the indicator amino acid oxidation, and more practical experimental diets were used to empirically determine the Met requirement, a greater estimate was found in adult cats (Pezzali et al., 2022) and adult Labrador Retrievers and Beagles, but not in miniature Dachshunds (Mansilla et al., 2020), compared with the current recommendations proposed by the NRC (2006) and AAFCO (2023). Harrison et al. (2020), however, found a decreased estimate for the Met requirement in adult Labrador Retrievers compared with Mansilla et al. (2020), likely due to differences in experimental design and diet composition. This discrepancy highlights the need to standardize methodologies and to investigate the effect of other dietary factors on the Met and total sulfur amino acid requirements owing to their complex metabolism. Indeed, the metabolism of sulfur amino acids may differ between dog breeds, resulting in differences in Met requirements and potentially making taurine a conditionally indispensable amino acid in some breeds under specific dietary conditions. While dietary Met is usually provided in excess in commercial diets, excess of Met may lead to hyperhomocysteinemia, which has been associated with detrimental health effects in other species. Due to the complex metabolism of sulfur amino acids and the complex matrix of pet foods, determining the ideal provision of sulfur amino acids in commercial diets to maximize animal health is a challenge. More long-term studies investigating the effect of different doses and ratios (Met:Cys) of sulfur amino acids on overall metabolism are necessary, in addition to requirement studies, to optimize the dietary delivery of sulfur amino acids in dogs and cats.
30
Kapravelou, Garyfallia, Rosario Martínez, Jole Martino, Jesus M. Porres, and Ignacio Fernández-Fígares. "Natural Fermentation of Cowpea (Vigna unguiculata) Flour Improves the Nutritive Utilization of Indispensable Amino Acids and Phosphorus by Growing Rats." Nutrients 12, no. 8 (July 23, 2020): 2186. http://dx.doi.org/10.3390/nu12082186.
Анотація:
Cowpea (Vigna unguiculata) is among the most cultivated legumes, with interesting agronomic and environmental properties, and great potential as a nutritious food. The nutritional value of cowpea can be improved by technological processing. In this study, we showed that natural fermentation improved bioavailability of protein, amino acids, and dietary essential minerals from cowpea in growing rats, thus strengthening its potential value as functional food or food supplement. Forty Wistar albino rats (48 ± 1.8 g), were fed one of four experimental diets (n = 10 rats per diet): casein, raw cowpea, fermented cowpea or fermented and autoclaved cowpea. Despite lower growth indices of raw and fermented cowpea protein (PER, FTI) than casein, fermentation enhanced apparent digestibility of arginine, leucine, lysine, methionine, phenylalanine, tyrosine, and valine, and true digestibility of essential amino acids, except for tyrosine and valine, compared to raw cowpea. On the other hand, autoclaving of fermented cowpea flour decreased apparent, as did true digestibility of sulfur amino acids. Regarding the nutritive utilization of dietary essential minerals, Vigna unguiculata was a good source of available P, Mg, and K, while fermentation significantly improved the availability of P. Overall, cowpea was a good source of digestible essential amino acids and minerals and fermentation significantly improved its nutritional value that was not further enhanced by autoclaving.
31
van den Borne, Joost J. G. C., Sven J. J. Alferink, Marcel J. W. Heetkamp, Antoon A. A. Jacobs, Martin W. A. Verstegen, and Walter J. J. Gerrits. "Asynchronous Supply of Indispensable Amino Acids Reduces Protein Deposition in Milk-Fed Calves." Journal of Nutrition 142, no. 12 (October 17, 2012): 2075–82. http://dx.doi.org/10.3945/jn.112.165670.
32
Kawano-Kawada, Miyuki, Haruka Ichimura, Shota Ohnishi, Yusuke Yamamoto, Yumi Kawasaki, and Takayuki Sekito. "Ygr125w/Vsb1-dependent accumulation of basic amino acids into vacuoles of Saccharomyces cerevisiae." Bioscience, Biotechnology, and Biochemistry 85, no. 5 (January 28, 2021): 1157–64. http://dx.doi.org/10.1093/bbb/zbab015.
Анотація:
ABSTRACT The Ygr125w was previously identified as a vacuolar membrane protein by a proteomic analysis. We found that vacuolar levels of basic amino acids drastically decreased in ygr125wΔ cells. Since N- or C-terminally tagged Ygr125w was not functional, an expression plasmid of YGR125w with HA3-tag inserted in its N-terminal hydrophilic region was constructed. Introduction of this plasmid into ygr125w∆ cells restored the vacuolar levels of basic amino acids. We successfully detected the uptake activity of arginine by the vacuolar membrane vesicles depending on HA3-YGR125w expression. A conserved aspartate residue in the predicted first transmembrane helix (D223) was indispensable for the accumulation of basic amino acids. YGR125w has been recently reported as a gene involved in vacuolar storage of arginine; and it is designated as VSB1. Taken together, our findings indicate that Ygr125w/Vsb1 contributes to the uptake of arginine into vacuoles and vacuolar compartmentalization of basic amino acids.
33
Danilova, I. S. "Influence of heat treatment on amino acid composition of snails meat." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 20, no. 88 (November 13, 2018): 69–71. http://dx.doi.org/10.32718/nvlvet8812.
Анотація:
Amino acids are organic compounds that are the structural component of the protein. That is, protein is the main building material of the tissues of the body. It is needed for muscle mass growth and is indispensable in fat burning – all about amino acids, of which protein is formed. The main source of amino acids are food that is rich in protein. However, based on the content of one or another amino acid proteins contained in food, can be divided into complete and inferior. Complete proteins contain all the essential amino acids. These products include, primarily, products of animal origin: meat, poultry, fish, eggs, dairy products. In order to plant sources of full protein is soybean. Inferior proteins – in their composition there is at least one irreplaceable acid. Accordingly, in their “quality” defective proteins can be very different. The source of defective proteins is mainly products of planting: legumes, cereals, nuts and seeds. The purpose of our work was to determine the content of amino acids in raw and cooked meat of the food species Helix pomatia, Helix aspersa maxima and Helix aspersa muller and to make an analysis on the effect of heat treatment on the amino acid composition of snail meat. This article presents the results of the content of amino acids in meat of food snails using electrophoresis “Capel-105/105M”. Three types of snails have identified the presence and amount of amino acids. In general, the meat of snails contains amino acids – arginine, histidine, serine, alanine, glycine, tyrosine and proline, and irreplaceable – lysine, phenylalanine, leucine + isoleucine, methionine, valine, threonine.
34
Mafu, Ampaka, Sunantha Ketnawa, Suphat Phongthai, Regine Schönlechner, and Saroat Rawdkuen. "Whole Wheat Bread Enriched with Cricket Powder as an Alternative Protein." Foods 11, no. 14 (July 19, 2022): 2142. http://dx.doi.org/10.3390/foods11142142.
Анотація:
The current market trends in modern sedentary lifestyles drive the development of new functional products able to fulfill consumers’ demand for a healthy diet. Whole wheat bread contains more protein and fiber than white bread; however, it could be improved in terms of protein content and quality. Cricket powder, which contains high protein (55.11, wt%), could be used as an alternative source to tackle those deficiencies in such bread. Hence, the study aimed to apply cricket powder in the whole wheat bread formula to enrich protein content, indispensable amino acids and determine their physico-chemical properties, consumers’ acceptance, and shelf-life storage. The results showed that all enriched bread presented high protein (18.97–25.94, wt%), fat (10.91–15.07, wt%), and ash (2.09–2.33, wt%) with the increment of the cricket powder than those of the control bread. Enriched breads’ crust colors were not significantly different (p > 0.05), while crumb colors were darker (L* = 55.64–64.48) compared to the control (L* = 69.98). Enriched bread had a hard texture and required a lot of chewing force compared to the control. Furthermore, all samples yielded a shelf-life of 5 days when monitoring the mold growth. From the results, the bread enriched with 20% cricket powder yielded the best consumers’ acceptance score of 77%. It was predominantly high in indispensable amino acids such as leucine, phenylalanine, lysine, and arginine. Therefore, cricket powder could be a novel alternative protein source and successfully utilized in whole wheat bread to enhance its protein content and indispensable amino acids with consumers’ acceptance responding to the current market trend.
35
Yoon, Mee-Sup, Guangwei Du, Jonathan M. Backer, Michael A. Frohman, and Jie Chen. "Class III PI-3-kinase activates phospholipase D in an amino acid–sensing mTORC1 pathway." Journal of Cell Biology 195, no. 3 (October 24, 2011): 435–47. http://dx.doi.org/10.1083/jcb.201107033.
Анотація:
The rapamycin-sensitive mammalian target of rapamycin (mTOR) complex, mTORC1, regulates cell growth in response to mitogenic signals and amino acid availability. Phospholipase D (PLD) and its product, phosphatidic acid, have been established as mediators of mitogenic activation of mTORC1. In this study, we identify a novel role for PLD1 in an amino acid–sensing pathway. We find that amino acids activate PLD1 and that PLD1 is indispensable for amino acid activation of mTORC1. Activation of PLD1 by amino acids requires the class III phosphatidylinositol 3-kinase hVps34, which stimulates PLD1 activity through a functional interaction between phosphatidylinositol 3-phosphate and the Phox homology (PX) domain of PLD1. Furthermore, amino acids stimulate PLD1 translocation to the lysosomal region where mTORC1 activation occurs in an hVps34-dependent manner, and this translocation is necessary for mTORC1 activation. The PX domain is required for PLD1 translocation, mTORC1 activation, and cell size regulation. Finally, we show that the hVps34-PLD1 pathway acts independently of, and in parallel to, the Rag pathway in regulating amino acid activation of mTORC1.
36
Schaafsma, Gertjan. "Advantages and limitations of the protein digestibility-corrected amino acid score (PDCAAS) as a method for evaluating protein quality in human diets." British Journal of Nutrition 108, S2 (August 2012): S333—S336. http://dx.doi.org/10.1017/s0007114512002541.
Анотація:
PDCAAS is a widely used assay for evaluating protein quality. It is a chemical score, which is derived from the ratio between the first limiting amino acid in a test protein and the corresponding amino acid in a reference amino acid pattern and corrected for true faecal N digestibility. Chemical scores exceeding 100 % are truncated to 100 %. The advantages of the PDCAAS are its simplicity and direct relationship to human protein requirements. The limitations are as follows: the reference pattern is based on the minimum amino acid requirements for tissue growth and maintenance and does not necessarily reflect the optimum intake. Truncated PDCAAS of high-quality proteins do not give any information about the power of these proteins to compensate, as a supplement, for low levels of dietary essential amino acids in low-quality proteins. It is likely that faecal N digestibility does not take into account the loss from the colon of indispensable amino acids that were not absorbed in the ileum. Anti-nutritional factors, such as lectins and trypsin inhibitors, in several plant protein sources can cause heightened endogenous losses of amino acids, an issue which is particularly relevant in animal feedstuffs. The assumption that amino acid supplementation can completely restore biological efficiency of the protein source is incorrect since the kinetics of digestion and absorption between supplemented free amino acids and amino acids present in dietary proteins, are different.
37
Kashyap, Sindhu, Nirupama Shivakumar, Aneesia Varkey, Rajendran Duraisamy, Tinku Thomas, Thomas Preston, Sarita Devi, and Anura V. Kurpad. "Ileal digestibility of intrinsically labeled hen's egg and meat protein determined with the dual stable isotope tracer method in Indian adults." American Journal of Clinical Nutrition 108, no. 5 (October 1, 2018): 980–87. http://dx.doi.org/10.1093/ajcn/nqy178.
Анотація:
ABSTRACTBackgroundProtein quality assessment through the Digestible Indispensable Amino Acid Score requires accurate measurements of true ileal protein and amino acid digestibility, for which a dual isotope technique was recently developed. However, the ileal digestibility of indispensable amino acids (IAA) in humans from high-quality proteins is not well known.ObjectiveThe aim of this study was to intrinsically label hen's egg and meat protein by the use of uniformly 2H-labeled amino acids, and to measure their true ileal indispensable amino acid (IAA) digestibility via the dual isotope method in humans.Design2H-labeled lyophilized boiled egg white protein, whole boiled egg, and cooked meat were obtained from layer hens (BV-300) administered a uniformly 2H-labeled amino acid mix orally for 35 d with their daily feed. The ileal IAA digestibility of these proteins was determined with reference to digestibility of previously characterized [U-13C]spirulina in a dual tracer method in healthy Indian subjects whose intestinal health was measured by the plasma kynurenine-to-tryptophan (KT) ratio.ResultsAll subjects had normal KT ratios. The mean ± SD true ileal IAA digestibility of 2H-labeled egg white protein, whole boiled egg, and cooked meat was 86.3% ± 4.6%, 89.4% ± 4.5%, and 92.0% ± 2.8%, respectively. Leucine digestibility correlated with the KT ratio (r = −0.772; P = 0.009).ConclusionsUniformly 2H-labeled hen's egg and meat protein can be used to measure ileal IAA digestibility by the dual isotope tracer approach in humans. The mean IAA digestibility values for these high-quality proteins in the healthy Indians studied were similar to values obtained in earlier human and animal experiments. Leucine digestibility in these meal matrices correlated with the KT ratio, but this aspect needs further evaluation. This trial was registered at the Clinical Trials Registry of India (http://ctri.nic.in) as CTRI/2018/03/012265.
38
Canada, Paula, Sofia Engrola, Nadège Richard, Ana Filipa Lopes, Wilson Pinto, Luísa M. P. Valente, and Luís E. C. Conceição. "Dietary indispensable amino acids profile affects protein utilization and growth of Senegalese sole larvae." Fish Physiology and Biochemistry 42, no. 6 (May 17, 2016): 1493–508. http://dx.doi.org/10.1007/s10695-016-0235-1.
39
Zhou, Wu, Yuwei Wang, Fang Yang, Qi Dong, Honglun Wang, and Na Hu. "Rapid Determination of Amino Acids of Nitraria tangutorum Bobr. from the Qinghai-Tibet Plateau Using HPLC-FLD-MS/MS and a Highly Selective and Sensitive Pre-Column Derivatization Method." Molecules 24, no. 9 (April 28, 2019): 1665. http://dx.doi.org/10.3390/molecules24091665.
Анотація:
Amino acids are indispensable components of living organisms. The high amino acid content in Nitraria tangutorum Bobr. fruit distinguishes it from other berry plants and is of great significance to its nutritional value. Herein, using 10-ethyl-acridine-3-sulfonyl chloride as a fluorescent pre-column labeling reagent, a method for the efficient and rapid determination of amino acid content in N. tangutorum by pre-column fluorescence derivatization and on-line mass spectrometry was established and further validated. The limits of detection (signal-to-noise ratio = 3) were between 0.13 and 1.13 nmol/L, with a linear coefficient greater than 0.997 and a relative standard deviation between 1.37% and 2.64%. In addition, the method required a short analysis time, separating 19 amino acids within 20 min. Subsequently, the method was used to analyze the amino acid content of Nitraria tangutorum Bobr. from tissues retrieved from seven regions of the Qinghai-Tibet Plateau. Nitraria tangutorum Bobr. was shown to contain a large amount of amino acids, with the total content and main amino acid varying between the different tissues. This research supports the nutritional evaluation, quality control, and development and utilization of Nitraria tangutorum Bobr.
40
Yin, Fugui, Zhenzhen Zhang, Ju Huang, and Yulong Yin. "Digestion rate of dietary starch affects systemic circulation of amino acids in weaned pigs." British Journal of Nutrition 103, no. 10 (January 27, 2010): 1404–12. http://dx.doi.org/10.1017/s0007114509993321.
Анотація:
The present study was conducted to evaluate the in vitro and in vivo digestibility of dietary starch and its digestive behaviour on the systemic circulating amino acids (AA) in weaned pigs. Eighteen weanling pigs surgically fitted with a catheter in the jugular vein were randomly assigned to three dietary treatment groups. Sticky rice starch (SRS) was hydrolysed more quickly in vitro (P < 0·05) than maize starch (MS) and resistant starch (RS), and was almost completely hydrolysed within 4 h. The in vivo digestibility of dietary starch in different segments of the small intestine was significantly different. SRS was digested (81·9 %; P < 0·05) in the anterior jejunum, but not more than half of the MS and RS was digested in the same segment of the small intestine. The digestibilities of isoleucine, leucine, methionine, phenylalanine, threonine, tryptophan, valine, alanine, aspartate and serine in the SRS group were higher than in the MS group (P < 0·05), and all nutritionally indispensable and dispensable AA in the SRS group were higher when compared with those in the RS group (P < 0·05). The serum concentrations of nutritionally indispensable AA, proline and serine in the three groups were increased to a peak point within 1·5 h postprandially then decreased gradually; however, the time that serum concentrations of alanine, aspartate, glutamate and glycine in each group increased to a peak point was different. The concentrations of nutritionally indispensable AA, including arginine, cystine, histidine, isoleucine, leucine, methionine, phenylalanine, threonine, tryptophan, tyrosine and valine at 09.30 hours and arginine, cystine, histidine, isoleucine, methionine, phenylalanine, threonine, tryptophan, tyrosine and valine at 13.30 hours in the SRS group were higher than in the MS group (P < 0·05); all nutritionally indispensable AA in the SRS group were higher than in the RS group at 09.30 and 13.30 hours (P < 0·05), respectively. We conclude that dietary starches digested rapidly in vitro have higher digestibility in the anterior small intestine of pigs. Diets containing rapidly digestible starch ameliorate the digestive and absorptive function and regulate AA metabolism to beneficially increase the entry of dietary AA into the systemic circulation in pigs.
41
Boila, R. J., S. C. Stothers, and L. D. Campbell. "The relationships between the concentrations of individual amino acids and protein in wheat and barley grain grown at selected locations throughout Manitoba." Canadian Journal of Animal Science 76, no. 2 (June 1, 1996): 163–69. http://dx.doi.org/10.4141/cjas96-025.
Анотація:
The concentrations of protein and individual amino acids were determined in the grain from three cultivars of wheat and three cultivars of barley, each grown at 12 locations throughout Manitoba over 3 consecutive years. Protein concentration differed (P < 0.05) among the cultivars of wheat but not (P > 0.05) among cultivars of barley. Although the concentrations of several amino acids differed (P < 0.05) among cultivars of wheat or barley the differences among cultivars of each grain were not considered to be critical in relation to the requirements for indispensable amino acids for swine or poultry. The percentage of an ammo acid in the DM of wheat and barley increased (P < 0.05) linearly with an increase in percentage of protein in the grain. The wide range of r2 (0.29 to 0.88) obtained for this amino acid-protein relationship may be due to the different effect of environment (location and year of growth) on the concentration of individual amino acids, compared to protein, among cultivars of wheat or barley. The percentage of total variance due to an interaction between cultivar and environment was low for protein but was several fold higher for individual amino acids. The error in the prediction of amino acid concentrations with regression equations may be no different than the error associated with predicting the mean concentrations of amino acids in the protein of wheat or barley as g (100 g protein)−1, and obtained from tabulations of analyses. However, regression equations for lysine in wheat and barley did account for the significant (P < 0.05) decrease in concentration of lysine in the protein as the percentage of protein in these grains increased. Key words: Grain, wheat, barley, protein, amino acids, Manitoba
42
Cunnane, Stephen C. "The conditional nature of the dietary need for polyunsaturates: a proposal to reclassify ‘essential fatty acids’ as ‘conditionally-indispensable’ or ‘conditionally-dispensable’ fatty acids." British Journal of Nutrition 84, no. 6 (December 2000): 803–12. http://dx.doi.org/10.1017/s0007114500002415.
Анотація:
The term essential fatty acid no longer clearly identifies the fatty acids it was originally used to describe. It would be more informative if the concept of essentiality shifted away from the symptoms arising from the lack of de novo synthesis of linoleate or α-linolenate and towards the adequacy of the capacity for synthesis and conservation of both the parent and the derived long-chain polyunsaturates. For instance, despite the existence of the pathway for synthesis of docosahexaenoate from α-linolenate, the former would be more correctly classified as ‘conditionally indispensable’ because the capacity of the pathway appears insufficient during early development, although it may be sufficient later in life in healthy individuals. Similarly, despite the inability to synthesize linoleate de novo, abundant linoleate stores and its relatively slow turnover in healthy adults probably makes linoleate ‘conditionally dispensable’ for long periods. There are two other anomalies with the terms essential and non-essential fatty acids: (1) under several different experimental circumstances, the C-skeleton of essential fatty acids is avidly used in the synthesis of non-essential fatty acids; (2) to function normally, the brain is required to endogenously synthesize several non-essential fatty acids. As with essential amino acids, which have been reclassified as indispensable or conditionally indispensable, such a change in terminology should lead to an improved understanding of the function and metabolism of polyunsaturates in particular, and long-chain fatty acids in general.
43
RITCHIE, James W. A., and Peter M. TAYLOR. "Role of the System L permease LAT1 in amino acid and iodothyronine transport in placenta." Biochemical Journal 356, no. 3 (June 8, 2001): 719–25. http://dx.doi.org/10.1042/bj3560719.
Анотація:
The feto-placental unit relies on a maternal supply of indispensable amino acids and iodothyronines for early development and normal growth. We examined the role of the System L transporter in placental uptake of these substances, using the human placental choriocarcinoma cell line BeWo as a model experimental system. BeWo cells express both heavy (4F2hc) and light (LAT1, LAT2) chains of the System L holotransporter. Saturable transport of both l-[3H]tryptophan and [125I]tri-iodo-l-thyronine in BeWo cells includes components sensitive to inhibition by the System-L-specific substrate 2-endoamino-bicycloheptane-2-carboxylic acid; kinetic properties of these components indicate that the 4F2hc-LAT1 transporter isoform is likely to predominate for the carriage of both substances at physiologically relevant concentrations. Both 4F2hc and LAT1 proteins are also expressed in human placental membranes and LAT1 at least is localized largely to the syncytiotrophoblast layer of the term human placenta. The 4F2hc-LAT1 transporter might therefore serve a vital role in supplying the developing fetus and the placenta with both thyroid hormones and indispensable amino acids from the maternal circulation.
44
Tamanna, Nahid, and Niaz Mahmood. "Emerging Roles of Branched-Chain Amino Acid Supplementation in Human Diseases." International Scholarly Research Notices 2014 (November 12, 2014): 1–8. http://dx.doi.org/10.1155/2014/235619.
Анотація:
The branched-chain amino acids (BCAAs), namely, valine, leucine, and isoleucine, are indispensable amino acids required for body protein synthesis. Unlike other amino acids, the BCAAs are primarily catabolised in the extrahepatic tissues. The BCAAs play role in regulation of protein synthesis and turnover as well as maintenance of the body glutamate-glutamine level. In strenuous and traumatic conditions, the BCAAs are oxidized which limits their availability in tissues. Such condition affects the body glutamate-glutamine pool and protein synthesis mechanisms. Thus BCCA supplementation is emerging as a nutritional strategy for treating many diseases. Many studies have found that BCAA administration is able to improve the health status of the patients suffering from different diseases even though there are conditions where they do not exert any effect. There are also some reports where elevated BCAAs have been shown to be associated with the pathogenesis of diseases. In this review, we have discussed the implication of BCAA supplementation in different pathological conditions and their relevant outcomes.
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Han, Fei, Paul James Moughan, Juntao Li, Natascha Stroebinger, and Shaojie Pang. "The Complementarity of Amino Acids in Cooked Pulse/Cereal Blends and Effects on DIAAS." Plants 10, no. 10 (September 24, 2021): 1999. http://dx.doi.org/10.3390/plants10101999.
Анотація:
The aim was to study the complementary effect between cereals and pulses on protein quality. The values for the digestible indispensable amino acid score (DIAAS) in cooked cereals and pulses, given alone, and blends of cooked cereals and pulses, were determined. True ileal digestibility (TID) values of amino acids for adult humans were obtained. It is difficult to determine ileal amino acid digestibility in humans directly, and for this reason, the growing pig is often used to obtain such values, as a preferred animal model. Seven growing pigs fitted with a T-cannula at the terminal ileum were allotted to a 7 × 6 incomplete Latin square with seven semi-synthetic diets (cooked mung bean, adzuki bean, millet, adlay, mung bean + millet, adzuki bean + adlay, and an N-free diet) and six 7-day periods. The mean TID values for crude protein differed significantly (p < 0.05), with millet having the highest digestibility (89.4%) and the adzuki bean/adlay mixture having the lowest (79.5%). For lysine, adzuki bean had the highest TID (90%) and millet had the lowest (70%). For the mean of all the amino acids, there was a significant (p < 0.05) effect of diet, with the TID ranging from 72.4% for the adzuki bean/adlay mixture to 89.9% for the adzuki beans. For the older child, adolescent, and adult, the DIAAS (%) was 93 for mung beans, 78 for adzuki beans, 22 for millet, 16 for adlay, and 66 for mung beans + millet, and 51 for adzuki beans + adlay. For mung beans, valine was first-limiting, and the SAA for adzuki beans, while lysine was first-limiting for the other foods. Chinese traditional diets, containing both cereals and pulses, are complementary for most, but not all of the indispensable amino acids.
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Blachier, François, Anne Blais, Rajavel Elango, Kuniaki Saito, Yoshiharu Shimomura, Motoni Kadowaki, and Hideki Matsumoto. "Tolerable amounts of amino acids for human supplementation: summary and lessons from published peer-reviewed studies." Amino Acids 53, no. 9 (August 2, 2021): 1313–28. http://dx.doi.org/10.1007/s00726-021-03054-z.
Анотація:
AbstractAmino acid supplementation may be indicated to correct for insufficient amino acid intake in healthy individuals, and in specific physiological or pathophysiological situations. However, there is a concern to not supplement beyond the tolerable upper intake level (UL) by determining parameters of no-observed-adverse-effect level (NOAEL) or lowest-observed-adverse-effect level (LOAEL) for each amino acid. Since the NOAEL and LOAEL values are at least one order of magnitude different when comparing the values obtained in rats and humans, the aim of this review is to evaluate to what extent the amino acid UL measured in the rat model, when referenced to the dietary usual consumption (UC) and dietary requirement (RQ) for indispensable amino acids, may be used as an approximation of the UL in humans. This review then compares the ratios of the NOAEL or LOAEL over UC and RQ in the rat model with the same ratios calculated in humans for the nine amino acids (arginine, serine, glycine, histidine, leucine, lysine, methionine, phenylalanine, and tryptophan) for which this comparison can be done. From the calculations made, it appears that for these 9 amino acids, the calculated ratios for rats and humans, although rather different for several amino acids, remains for all of them in the same order of magnitude. For tryptophan, tyrosine, and valine, the ratios calculated in rats are markedly different according to the sex of animals, raising the view that it may be also the case in humans.
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Fu, Shuang, Yurong Ma, Yinan Wang, Chongzhen Sun, Feng Chen, Ka-Wing Cheng та Bin Liu. "Contents and Correlations of Nε-(carboxymethyl)lysine, Nε-(carboxyethyl)lysine, Acrylamide and Nutrients in Plant-Based Meat Analogs". Foods 12, № 10 (12 травня 2023): 1967. http://dx.doi.org/10.3390/foods12101967.
Анотація:
High temperatures applied in the production of plant-based meat analogs (PBMA) lead to the occurrence of Maillard reactions, in which harmful compounds Nε-(carboxymethyl)lysine (CML), Nε-(carboxyethyl)lysine (CEL) and acrylamide are formed. However, little research has focused on these compounds in PBMA. In this study, the contents of CML, CEL and acrylamide in 15 commercial-sold PBMA were determined by an ultra-high performance liquid chromatograph coupled with a triple quadrupole tandem mass spectrometer (UHPLC-QqQ-MS/MS). Nutrients (protein, amino acids, fatty acids and sugars) which are related to the formation of these compounds were also studied. The results showed that CML, CEL and acrylamide contents were in the range of 16.46–47.61 mg/kg, 25.21–86.23 mg/kg and 31.81–186.70 μg/kg, respectively. Proteins account for 24.03–53.18% of PBMA. Except for Met + Cys, which is the limiting amino acid of most PBMA, all other indispensable amino acids met the requirements for adults. Besides, PBMA had more n-6 fatty acids than n-3 fatty acids. A correlation analysis showed that proteins and the profiles of amino acid and fatty acid had little influence on CML but significant influence on CEL and acrylamide. The results of the present study can be used as a reference to produce PBMA with higher amounts of nutrients and lower amounts of CML, CEL and acrylamide.
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Schinckel, A. P., M. E. Einstein, K. M. Ajuwon, and O. Adeola. "Characterization of whole body compositional growth of male ducks during the twenty-nine day post-hatch period." Canadian Journal of Animal Science 93, no. 1 (March 2013): 113–22. http://dx.doi.org/10.4141/cjas2012-026.
Анотація:
Schinckel, A. P., Einstein, M. E., Ajuwon, K. M. and Adeola, O. 2013. Characterization of whole body compositional growth of male ducks during the twenty-nine day post-hatch period. Can. J. Anim. Sci. 93: 113–122. Changes in whole body dry matter, lipid, ash, energy, crude protein, and amino acids were evaluated during a 29 d post-hatch period in White Pekin ducks. Drakes were assigned to slaughter 1, 8, 15, 22, or 29 d post-hatch with four replicates of four ducks per slaughter period. The body weight (BW) data were fitted to the Weibull function with the form:[Formula: see text]where BWit is the BW of the ith duck at t days of age and A, B, C, and IP are parameters. The value of IP, the inflection point, which minimized the residual SD, was 40 d. Values of A (8591 g, SE=190), B (42.87, SE=11.5), and C (1.7399, SE=0.050) resulted in an R 2 of 0.9836 and residual SD of 83.7 g. Allometric (Y=A BWB), linear-quadratic and exponential (Y=exp (b0+b1BW+b2 (BW)2) functions of BW were fitted to the chemical component and amino acid mass data. Dry matter percentage of the ducks increased (P<0.01) with age. The protein content of the dry matter decreased (P<0.01) from day 1 to day 8 (69 to 58.2%) and then increased to 60% by d 29. Concentrations of several amino acids were affected (P<0.05) by age. The predicted accretion rates of Lys, Trp, and Met relative to protein accretion increased as age increased. The predicted daily accretion rates for major indispensable amino acids increased rapidly the first 5 d post-hatch and subsequently increased but at a decreasing rate to day 29 post-hatch. The relative growth rates of chemical components and indispensable amino acids were affected by age indicating that the nutrient requirements of ducks differ from day 1 to day 29 post-hatch. Compositional growth and amino acid accretion data can be used to model the nutrient requirements of ducks.
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Cooper, Leah, Ronald O. Ball, Paul B. Pencharz, Ryosei Sakai, and Rajavel Elango. "Dispensable Amino Acids, except Glutamine and Proline, Are Ideal Nitrogen Sources for Protein Synthesis in the Presence of Adequate Indispensable Amino Acids in Adult Men." Journal of Nutrition 150, no. 9 (July 7, 2020): 2398–404. http://dx.doi.org/10.1093/jn/nxaa180.
Анотація:
ABSTRACT Background Nutritionally, there is a dietary requirement for indispensable amino acids (IAAs) but also a requirement for nitrogen (N) intake for the de novo synthesis of the dispensable amino acids (DAAs). It has been suggested that there might be a dietary requirement for specific DAAs. Objectives Experiment 1 tested whether 9 of the DAAs (Ala, Arg, Asn, Asp, Gln, Glu, Gly, Pro, Ser) are ideal N sources using the indicator amino acid oxidation (IAAO) technique. Experiment 2 examined whether there is a dietary requirement for Glu in adult men. Methods Seven healthy men (aged 20–24 y) participated in 11 or 2 test diet intakes, in experiment 1 and 2, respectively, in a repeated measures design. In experiment 1, a base diet consisting of the IAA provided at the RDA was compared with test intakes with the base diet plus addition of individual DAAs to meet a 50:50 ratio of IAA:DAA on an N basis. In experiment 2, the diets corresponded to the amino acid pattern present in egg protein, in which all Glu and Gln was present as Glu, or removed, with Ser used to make the diets isonitrogenous. On each study day the IAAO protocol with l-[1-13C]phenylalanine was used to measure whole-body protein synthesis. Results In experiment 1, repeated measures ANOVA with post hoc multiple comparisons showed that 7 of the 9 DAAs (Ala, Arg, Asn, Asp, Glu, Gly, Ser) decreased IAAO significantly (P < 0.05) compared with base IAA diet, the exceptions being Gln and Pro. In experiment 2, a paired t test did not find significant (P > 0.05) differences in the IAAO in response to removal and replacement of Glu intake. Conclusions The results suggest that in healthy men most DAAs are ideal N sources for protein synthesis, in the presence of adequate IAAs, and that endogenous synthesis of Glu is sufficient. Registered clinicaltrials.gov identifier: NCT02009917.
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Haschke, Ferdinand, Nadja Haiden, and Sagar K. Thakkar. "Nutritive and Bioactive Proteins in Breastmilk." Annals of Nutrition and Metabolism 69, Suppl. 2 (2016): 16–26. http://dx.doi.org/10.1159/000452820.
Анотація:
Protein ingested with breast milk provides indispensable amino acids which are necessary for new protein synthesis for growth and replacement of losses via urine, feces, and the skin. Protein gain in the body of an infant is highest during the first months when protein concentrations in breast milk are higher than during later stages of lactation. Low-birth-weight infants have higher protein needs than term infants and need protein supplements during feeding with breastmilk. Based on our better understanding of protein evolution in breastmilk during the stages of lactation, new infant formulas with lower protein concentration but better protein quality have been created, successfully tested, and are now available in many countries. Besides providing indispensable amino acids, bioactive protein in breast milk can be broadly classified into 4 major functions, that is, providing protection from microbial insults and immune protection, aiding in digestive functions, gut development, and being carriers for other nutrients. Individual proteins and their proposed bioactivities are summarized in this paper in brief. Indeed, some proteins like lactoferrin and sIgA have been extensively studied for their biological functions, whereas others may require more data in support to further validate their proposed functions.