Добірка наукової літератури з теми "Inc Biologicals Unlimited"

Jay “Ding” Darling (1876-1962) was a newspaper editorial cartoonist and duck hunter. Because of his pro-conservation cartoons, he had become one America’s most prominent conservationists by the early 1930s. Joseph P. Knapp (1864-1951) was a prominent businessman, philanthropist, conservationist, and duck hunter who, like Darling, had become concerned about the decline of waterfowl populations. Both worked to reverse this duck decline. Darling was appointed chief of the Bureau of Biological Survey in 1934 by President Franklin Roosevelt. During his short tenure as its chief (1934-1935), he focused the Bureau’s mission more on wildlife conservation and he oversaw the expansion of the national wildlife refuge system. In 1930, Knapp founded the More Game Birds in America Foundation. This Foundation through its waterfowl surveys documented that western Canada was the major breeding ground of ducks in North America. This resulted in the Foundation establishing Ducks Unlimited, Inc. in the US and Ducks Unlimited (Canada) in 1937.

J ay “Ding” Darling (1876-1962) was a newspaper editorial cartoonist and duck hunter. Because of his proconservation cartoons, he had become one America’s most prominent conservationists by the early 1930s. Joseph P. Knapp (1864-1951) was a prominent businessman, philanthropist, conservationist, and duck hunter who, like Darling, had become concerned about the decline of waterfowl populations. Both worked to reverse this duck decline. Darling was appointed chief of the Bureau of Biological Survey in 1934 by President Franklin Roosevelt. During his short tenure as its chief (1934-1935), he focused the Bureau’s mission more on wildlife conservation and he oversaw the expansion of the national wildlife refuge system. In 1930, Knapp founded the More Game Birds in America Foundation. This Foundation through its waterfowl surveys documented that western Canada was the major breeding ground of ducks in North America. This resulted in the Foundation establishing Ducks Unlimited, Inc. in the US and Ducks Unlimited (Canada) in 1937. DU, Inc. would raise money, and DU (Canada) would spend this money in western Canada on wetland conservation and restoration projects. Both men helped to slow down the loss of wetlands by stressing the need for the public and private sectors to conserve and restore them as waterfowl habitat. They also shaped future wetland science by creating opportunities for the employment of wetland scientists.

Abstract Introduction In myelodysplastic syndrome (MDS), multicolor flow cytometry (FC) offers a supplement to the morphological, cytogenetic and molecular evaluation of the hematopoietic lineages in the bone marrow (BM). In addition to providing a robust enumeration of immature myeloid cells, FC allows for identification of aberrant antigen expression patterns. While aberrant findings are well-defined in the myelo-monocytic lineages, the erythroid and megakaryocytic cell lineages are less well characterized by conventional FC. Imaging flow cytometry (IFC) is a novel methodology that bridge multicolor FC and high-throughput capacity with collection of multispectral imagery of single cells. This allows for the simultaneous evaluation, quantification and subsequent statistical analysis of both surface-, nuclear- and cytoplasmic immunophenotypic and morphometric characteristics on a practically unlimited number of cells in suspension. In this study, we hypothesized the IFC technique to be useful in discriminating dyserythropoietic from normal BM. Methods Mononuclear BM cells from 6 healthy donors and 2 MDS patients were thawed and stained with monoclonal antibodies against CD235a, CD45, CD71, CD117 and CD105. DRAQ5 was included for DNA staining, and Zombie Violet was included for viability. Data acquisition was performed on an ImageStream®XMarkII (Amnis/Merck), and subsequent analysis was done using the IDEAS software package. The median number of acquired cells was 130,000 for healthy BM (range: 70,548-300,000) and for MDS samples the equivalent number was 253,255 and 260,000, respectively. Nucleated erythroid cells (NECs) were identified based on the CD45lowDRAQ5highCD71+ immunophenotype and the coexpression of either CD235 and/or CD105. Next, proerythroblasts (ProE), basophilic erythroblasts (BasoE) and poly-orthochromatic erythroblasts (Poly_OrthoE) were distinguished based on the expression of CD117 and CD105 (Fig. 1, upper panel). Morphometric features were examined within function masks covering the cellular, nuclear and cytoplasmic area. From these masks we calculated cell area, nucleus area, nucleus elongatedness, nucleus circularity score, and nuclear location (delta centroid XY) (Fig. 1, lower panel). Data from healthy controls (Fig. 1A) served as biological reference for phenotypic and morphometric measurements of MDS samples. Results & perspectives In one MDS patient with single lineage dysplasia (MDS-SLD) (Fig. 1B), morphological dysplastic changes were seen in less than 10% of erythropoiesis, and manifested as nuclear lobulations, cytoplasmic granules and megaloblastic changes. We examined 6470 NECs by IFC and found an increased elongatedness and a decreased circularity score of the nuclei in the ProE population, reflecting irregular nuclear shape. Moreover, for the ProE and BasoE maturation stages, we detected increased cell size as well as enlarged nuclei of BasoE. This finding corresponded to the megaloblastic changes seen by morphology. Taken together, data from IFC confirmed the morphological findings in this MDS patient. The second patient (Fig. 1C) had MDS with excess blasts (MDS-EB-1). Morphologic examination showed mild dyserythropoiesis in less than 10% of erythropoietic cells with signs of multinuclearity, nuclear lobulations, and ringed sideroblasts. Interestingly, IFC analysis of 25,240 NECs identified aberrant delta centroid XY corresponding to decentralization of the nucleus in the ProE population, as also seen in the MDS-SLD patient. As such, with the applied IFC features, we were not able to detect changes corresponding to the morphological findings, which could be due to the low percentage of dysplastic erythroid cells. However, the deviant morphometric parameters detected by IFC in this patient identified additional dysmorphometric features of interest. To conclude, we provide proof-of-concept of a novel experimental approach to study and quantify morphometric changes in samples from MDS patients. Certainly, the technique needs to be explored in a larger patient material and we are currently extending our study cohort. Using a different-from-normal approach, our preliminary data was sufficient to demonstrate dysmorphometric changes in two MDS patients with only mild morphological dyserythropoiesis. Thus, we propose that IFC holds great promise as a powerful tool in the challenging setting of MDS diagnostics. Disclosures No relevant conflicts of interest to declare.

Marine seaweed bio-resources (MSBR) have long been recognized for their therapeutic and disease-prevention benefits. They are utilized to prevent various noncommunicable and communicable diseases as active components. In recent years, biotechnologists and pharmacologists have become interested in MSBR as a viable and almost limitless source of various biologically active compounds against a broad spectrum of diseases. The most recent global pandemic Covid-19 raised the scientific world's attention to discovering novel anti-viral agents against viruses. Oceans provide unlimited biological resources to develop therapeutic drugs for treating various human viral diseases. Our major intention of writing this chapter was to draw attention to the anti-viral potential of MSBR against various human viral diseases and gain the strength to conquer future viral pandemics.

Abstract Both normal and malignant cells are sensitive to high energy radiation. Although a long-term effect of high energy irradiation can be cancer induction, such radiation can also be used to treat cancer. Cancer is characterized by a pro gressive accumulation of malignant cells and the object of radiotherapy is to sterilize cancer cells so that they lose their capacity for unlimited proliferation. One of the important aims of radiation chemical and radiation biological studies is to provide information which may lead to the optimization of radiotherapy.

This chapter looks into the treatment of weapons under the two Additional Protocols of 1977. It defines a weapon in accordance with international law. The right of parties to use weapons is not unlimited due to applicable treaties and customary rules restricting armed conflict parties. Additionally, the use of lawful weapons must comply with the rules of distinction and proportionality in attacks, while inherently indiscriminate weapons like biological and nuclear weapons and other things that cause unnecessary suffering should never be used. The 1977 Additional Protocol I stipulated the prohibition of weapons causing widespread, severe, and long-term damage to the environment. The chapter then clarifies how cyber operations are not inherently indiscriminate. It also highlights the importance of verifying the legality of weapons.