Дисертації з теми "In vitro antithrombotic properties"
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Aggarwal, Rajesh Kumar. "The antithrombotic properties of polymer-coated, drug eluting coronary stents." Thesis, University of Leicester, 1996. http://hdl.handle.net/2381/34296.
Повний текст джерелаMnonopi, Nandipha Olivia. "In vitro testing to investigate the anticoagulant/antithrombotic and antidiabetic biological activity of Leonotis Leonurus." Thesis, Nelson Mandela Metropolitan University, 2007. http://hdl.handle.net/10948/693.
Повний текст джерелаHartley, Richard William. "The development of an in-vivo method for assessing the antithrombotic properties of pharmaceutical compounds." Thesis, Loughborough University, 1989. https://dspace.lboro.ac.uk/2134/12084.
Повний текст джерелаLai, Benjamin Fook Lun. "Bioactive polymers : a comparative study on the antithrombotic properties of soluble polymers and surface grafted polymers." Thesis, University of British Columbia, 2010. http://hdl.handle.net/2429/20871.
Повний текст джерелаTravers, M. "In vitro and clinical investigation of blood-membrane interactions : Influence on platelets and the immune system of membrane structure and antithrombotic agents." Thesis, University of Strathclyde, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382445.
Повний текст джерелаHuddie, P. L. "The properties of pituitary cells in vitro." Thesis, University of Newcastle Upon Tyne, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379245.
Повний текст джерелаSousa, Hiran Reis. "Investigação da atividade anti-agregante plaquetária in vitro de peptídeos inibidores da dissulfeto isomerase protéica - etapa 2." Universidade Federal do Maranhão, 2016. http://tedebc.ufma.br:8080/jspui/handle/tede/1632.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPQ)
Fundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão (FAPEMA)
Recent researches have emphasized the importance of redox mechanisms for platelet function modulation. The platelet surface contains a large variety of integrin receptors and other molecules presenting functional thiol groups in their structures, which are potential targets for redox regulation. Among these various thiol-containing proteins, integrin αIIbβ3 stands out for being the convergence path of platelet activation induced by various agonists. Activation of αIIbβ3 integrin is catalyzed by protein disulfide isomerase (PDI) through an essential conformational change leading to the exposure of fibrinogen-binding site. Thus, PDI has been shown to be an important target for the development of antiplatelet drugs. In recent years, many studies have described substances from plan (DE A. PAES et al., 2011), as well as synthetics that are capable of inhibiting PDI. In a previous study of our research group has shown that the synthetic peptide CxxC, which contains the redox motif of PDI in its original sequence CGHC, inhibited reductase activity of this enzyme, effect not observed with AxxA peptide, whose cysteines were replaced with alanine and Scr peptide, which contains the same aminoacids from CxxC peptide, but under random sequence. It has been also demonstrated that CxxC peptide was the only to reduce by 30% ADP-induced aggregation (5μM) in platelet rich plasma, an effect apparently mediated by the association of CxxC and PDI at platelet surface. Thus, in this work, we further assessed the effects of CxxC and its control peptides on platelet aggregation. Washed human platelets were incubated with CxxC peptide at concentrations of 3, 6 and 10 μM, resulting in a dose-dependent inhibition of maximum aggregation activated by thrombin (0.02 U/mL) at 25, 60 and 74%, respectively with IC50 of 6.13 ± 1.09 μM. The presence of control peptides did not produce any inhibitory effect. CxxC peptide also reduced the activation of αIIbβ3 integrin at platelet surface, but did not affect the expression of the markers CD 62-P and CD 63. Control peptides did not alter the expression of these markers. Analysis by mass spectrometry of the interaction of recombinant human PDI with the peptide showed that only CxxC peptide associated with the redox Cys400 of a’ motif of PDI, which has been considered essential for platelet aggregation. Together, these results demonstrate that CxxC peptide reduces platelet aggregation by association with PDI and can be further used as a model for the development of new antithrombotic drugs.
Investigações recentes têm enfatizado a importância de mecanismos redox na modulação da função plaquetária. A superfície da plaqueta contém grande variedade de integrinas e outras moléculas receptoras que possuem tióis funcionais em sua estrutura, os quais são alvos potenciais de regulação redox. Dentre estas várias proteínas tiólicas, a integrina αIIbβ3 destaca-se por ser a via de convergência da ativação plaquetária induzida por diversos agonistas. A ativação da integrina αIIbβ3 é catalisada pela proteína dissulfeto isomerase (PDI), essencial à mudança de conformação que leva à exposição do sitio de ligação ao fibrinogênio. Sendo assim, a PDI tem se mostrado como um alvo importante para o desenvolvimento de fármacos reguladores da agregação plaquetária. Nos últimos anos, diversos estudos têm descrito substâncias de origem vegetal, animal e sintéticas que são capazes de inibir a PDI. Em trabalho do nosso grupo de pesquisa (DE A. PAES et al., 2011), demonstrou que o peptídeo sintético CxxC, o qual contém o motivo redox da PDI na sua sequência original CGHC, inibiu a atividade redutase desta enzima; efeito não observado com os peptídeos AxxA, que possui as cisteínas substituídas por alanina e Scr, peptídeo controle contendo os mesmos aminoácidos do peptídeo CxxC, porém com sequência aleatória sem formação de ditiol. Demonstrou-se, também, que apenas o peptídeo CxxC reduziu em 30% a agregação induzida por ADP (5M) em plasma rico em plaquetas, efeito aparentemente mediado pela associação do CxxC com a PDI na superfície plaquetária. Sendo assim, neste trabalho continuamos a avaliação dos efeitos do peptídeo CxxC e seus controles sobre a agregação plaquetária. Para tanto, incubamos lavado de plaquetas humanas com o peptídeo CxxC nas concentrações de 3, 6 e 10 μM, resultando em inibição concentração-dependente da agregação ativada por trombina (0,02 U/mL) em 25, 60 e 74 %, respectivamente, com IC50 de 6,13 ± 1,09 μM. A presença dos peptídeos controle não produziu quaisquer efeitos inibitórios. O peptídeo CxxC reduziu a ativação da integrina αIIbβ3 na superfície da plaqueta, porém não impactou a expressão dos antígenos CD 62-P e CD 63. Os peptídeos controle não alteraram a expressão desses marcadores. A análise por espectrometria de massas da interação da PDI recombinante humana com os peptídeos, mostrou que apenas o peptídeo CxxC associa-se com a Cys400 do motivo redox a’ da hPDI, o qual tem sido considerado fundamental para a agregação plaquetária. Em conjunto, estes resultados demonstram que o peptídeo CxxC reduz a agregação plaquetária via associação com a PDI, podendo ser empregado como modelo para o desenvolvimento de fármacos novos antitrombogênicos.
Hammad, Mohammad. "In vitro properties of novel root canal filling materials." Thesis, University of Manchester, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.502267.
Повний текст джерелаMould, A. P. "The aggregation properties of type I procollagen in vitro." Thesis, University of Manchester, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.377670.
Повний текст джерелаZhang, Qing. "Role of Polymer Physicochemical Properties on in vitro Mucoadhesion." Scholarly Commons, 2020. https://scholarlycommons.pacific.edu/uop_etds/3698.
Повний текст джерелаCao, Yisi. "Analysis and properties of carotenoids in vivo and in vitro." FIU Digital Commons, 2007. http://digitalcommons.fiu.edu/etd/2033.
Повний текст джерелаCarracedo, Lucy Micaela. "Properties and mechanisms of the cortical delta rhythm in vitro." Thesis, University of Newcastle upon Tyne, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.548032.
Повний текст джерелаKonkel, Michael Edward. "In vitro analysis of the invasive properties of Campylobacter jejuni." Diss., The University of Arizona, 1990. http://hdl.handle.net/10150/185029.
Повний текст джерелаHaverkos, Stephen M. "FRICTIONAL PROPERTIES OF NOVEL BRACKET SYSTEMS: AN IN-VITRO STUDY." VCU Scholars Compass, 2019. https://scholarscompass.vcu.edu/etd/5770.
Повний текст джерелаFrei, Renate. "Comparison of cytotoxic properties of free and liposomal bisphosphonates in vitro." Zürich : ETH, Eidgenössische Technische Hochschule Zürich, Departement Chemie und Angewandte Biowissenschaften, 2005. http://e-collection.ethbib.ethz.ch/show?type=dipl&nr=175.
Повний текст джерелаYoung, Dominic. "Quantification of patellar tendon biomechanical properties, in vitro and in situ." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ38619.pdf.
Повний текст джерелаKing, Tanya Margaret. "Correlates between learning and the properties of the IMHV in vitro." Thesis, University of Newcastle Upon Tyne, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294374.
Повний текст джерелаChambers, Emma Sarah. "The immunomodulatory properties of vitamin D in vitro and in vivo." Thesis, King's College London (University of London), 2013. https://kclpure.kcl.ac.uk/portal/en/theses/the-immunomodulatory-properties-of-vitamin-d-in-vitro-and-in-vivo(23201010-6cc5-41e6-9498-f0bf36fb426b).html.
Повний текст джерелаDu, Plessis-Stoman Debbie. "An investigation of the in vitro anticancer properties of selected platinum compounds." Thesis, Nelson Mandela Metropolitan University, 2006. http://hdl.handle.net/10948/498.
Повний текст джерелаOliver, Michael W. "Electrophysiological properties of the hippocampal formation in rat : an in vitro study." Thesis, University of British Columbia, 1986. http://hdl.handle.net/2429/27503.
Повний текст джерелаMedicine, Faculty of
Cellular and Physiological Sciences, Department of
Graduate
Gershon, P. "Studies with P. falciparum in vitro : The antimalarial properties of antiribosomal antibiotics." Thesis, University of Liverpool, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356275.
Повний текст джерелаMcInnes, Fiona Jane. "In-vitro and In-vivo properties of a lyophilised nasal dosage system." Thesis, University of Strathclyde, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.275120.
Повний текст джерелаZuo, Tianming. "The In Vitro Cellular Uptake and Physiochemical Properties of Tocotrienols." [Johnson City, Tenn. : East Tennessee State University], 2003. https://dc.etsu.edu/etd/780.
Повний текст джерелаTitle from electronic t.p. ETSU ETD database URN: etd-0618103-114702. Includes bibliographical references. Also available via Internet at the UMI web site.
Le, Maux Solène. "β-lactoglobulin/linoleate complexes : binding properties and biological functions". Rennes, Agrocampus Ouest, 2013. http://www.theses.fr/2013NSARB238.
Повний текст джерелаFood structure can have a profound influence on delivering health benefits. Bioaccessibility of nutrients can be affected by their interaction with food components. The dairy protein β-lactoglobulin (βlg) is known to bind hydrophobic ligands such as fatty acids (FA). However, this protein is highly sensitive to the process conditions used in the dairy industry. Therefore βlg is often present in non-native or aggregated form in processed food. This structural change may modify the protein affinity for FA and the biological properties of the FA/protein complexes. The aim of this thesis was to investigate the interaction of bovine βlg in different structural forms (native, covalent dimer and nanoparticles) with linoleate (C18:2, cis,cis-9,12-octadecadienoic acid) and conjugated linoleic acids (CLA, C18:2), and the impact of those complexes on their biological activity in vitro. Two different sets of binding sites were determined for the interaction between linoleate and βlg, regardless of its state of aggregation, using intrinsic fluorescence spectroscopy and isothermal titration calorimetry. By increasing the level of βlg aggregation, the linoleate/βlg stoichiometry increased but the association constants remained similar for both sets of binding sites. In the presence of linoleate, the native protein was more sensitive to gastric in vitro digestion, due to the increased level of denaturation/aggregation of βlg. Transport of linoleate in Caco-2 cells was decreased in presence of the native βlg as observed by confocal microscopy and a monolayer that mimics the intestinal barrier. Cytotoxicity of linoleate on Caco-2 cells was reduced when the FA was bound to βlg compared to free FA. CLA, which is less water soluble than linoleate, is more cytotoxic when complexed by βlg than in its free form. Therefore, it is proposed that βlg can act as a molecular carrier and alter the bioaccessibility of FA depending on their solubility
Kulakowski, Guillaume. "Membrane binding properties of RAB GTPases." Thesis, Paris 6, 2017. http://www.theses.fr/2017PA066358/document.
Повний текст джерелаRAB GTPases are major regulators of vesicular trafficking and localize to specific compartments. Deciphering the molecular mechanisms governing RAB localization is thus critical to understand intracellular transport processes. We have managed, for the first time, to incorporate purified and prenylated RABs into artificial membranes. By doing so, we observed that RAB6, but not RAB1 or RAB5, is able to promote by itself vesicle tethering. We believe that RAB6 is able to interact in trans with itself and to consequently drive homotypic membrane tethering. In the main part of this study, we investigated the physicochemical membrane requirements necessary for RAB recruitment. RAB1, RAB5 and RAB6 were all found to only localize to disordered membrane domains and to preferentially bind to curved membranes. We demonstrated that this specific recruitment of RAB1, RAB5 and RAB6 is primarily dependent on the hydrophobic insertion of their prenyl group into lipid packing defects. In contrast, RAB35 recruitment was primarily dependent on the presence of negatively charged lipids and was found to be modulated, to a lesser extent, by lipid packing defects. Although RAB4 and RAB11 were effectively recruited to purified Golgi fractions, in an effector-independent manner, membrane charges and lipid packing defects were not sufficient to promote their recruitment to synthetic vesicles; suggesting that RAB4 and RAB11 require more demanding membrane physicochemical properties. Our work demonstrates that the properties of membranes are critical for the regulation of RAB specific membrane targeting
Wells, Jeffrey Donald. "The processing and in vitro degradation properties of gravity sintered calcium polyphosphate powders." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ29439.pdf.
Повний текст джерелаBray, Elen. "Analysis of mesenchymal stem cell properties in three dimensional in vitro growth environments." Thesis, University of York, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.516629.
Повний текст джерелаSun, Zheng, and 孙诤. "In vitro studies of antiglycoxidative properties of microalgal extracts and their active compounds." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46480638.
Повний текст джерелаLiu, Xuqing, and 刘绪卿. "Effects of activated microglia on the properties of neural stem cells in vitro." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B47236000.
Повний текст джерелаpublished_or_final_version
Anatomy
Doctoral
Doctor of Philosophy
Verschuren, Emma Wilhelmina. "Tumourigenic and tumour suppressive properties of KSHV cyclin in vitro and in vivo." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.405864.
Повний текст джерелаBai, Ke. "Spatial distribution, temporal development and mechanical properties of the endothelial glycocalyx in vitro." Thesis, Queen Mary, University of London, 2012. http://qmro.qmul.ac.uk/xmlui/handle/123456789/3182.
Повний текст джерелаAsia, Winifred. "An in-vitro study of the physical properties of core build-up materials." University of the Western Cape, 2017. http://hdl.handle.net/11394/6307.
Повний текст джерелаThe aim of this study was to evaluate and compare the physical properties of two core build-up materials (ParaCore and CoreXflow) and compare this to conventional composite material (Filtek Supreme Plus and SDR Flow) used as core build-up material.
Goswami, Tarun. "Sublingual drug delivery: In vitro characterization of barrier properties and prediction of permeability." Scholarly Commons, 2008. https://scholarlycommons.pacific.edu/uop_etds/2370.
Повний текст джерелаGoswani, Tarun. "Sublingual drug delivery : in vitro characterization of barrier properties and prediction of permeability." Scholarly Commons, 2008. https://scholarlycommons.pacific.edu/uop_etds/708.
Повний текст джерелаMulder, Riaan. "An in vitro study of the properties of GICs with bioactive biomaterial modification." University of the Western Cape, 2019. http://hdl.handle.net/11394/7038.
Повний текст джерелаThe fluoride release and chemical adherence to tooth structure remain the most desirable features of glass ionomer restorative cements (GICs). Although the physical properties for multi-surface restorations are well-defined, even with the introduction of newer GICs not all demands have been met. Yet, increased use of GICs will only be possible if clinicians change their perceptions of the low survival rate of GICs. The lower clinical success rate of GICs is partly due to the marginal integrity and wear over time, which has often been recorded in the literature as restoration failure. The current, well-established restorative options for the primary dentition are Resin Modified Glass Ionomers (RMGICs) and Compomer resins. There is a paradigm shift towards materials that are more biologically favourable. Areas of research for dental materials include antibacterial properties in conjunction with ion release to maintain healthy restored teeth. If a GIC can provide adequate physical properties with the inclusion of the aforementioned features, GICs might become a more viable permanent restorative solution.
McKay, J. S. "The influence of opiates on ion transport across rabbit ileal mucosa in vitro." Thesis, University of Aberdeen, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.377604.
Повний текст джерелаEltayeb, Aziz. "An in-vitro evaluation of the physical properties of a new bulk-fill composite." University of the Western Cape, 2017. http://hdl.handle.net/11394/5950.
Повний текст джерелаDental composite is a synthetic resin that is used as a tooth coloured restorative material in dentistry. It became the material of choice in the dental field due to its superiority in strength and aesthetics (Garcia et al., 2006). Composite resin has favourable physical properties such as high wear resistance and shade stability. The main disadvantages are as follows: polymerization shrinkage which leads to marginal leakage; discoloration of the filling; postoperative sensitivity; and recurrent caries (Garcia et al., 2006). In order to decrease the application time of incremental layering techniques in conventional resin composite restorations, bulk-fill composites were introduced to the dental market with modifications in physical and mechanical properties. BulkFill composite can be applied in a single one-step increment layer of 4 - 5mm, saving considerable time during the clinical procedure when compared to the conventional composite layering technique of 2 mm (Leprince et al., 2014).
Khair, Ro’aa Mohammed Jafar Mohammed Mohammed. "Comparative in vitro study of selected physical properties of Activa, Cention N and Vitremer." University of the Western Cape, 2021. http://hdl.handle.net/11394/8144.
Повний текст джерелаBackground: This study aimed to determine the association between dimensional change and surface roughness (Ra) of Vitremer, Activa and Cention N after immersing them into two different media: acidic and artificial saliva media for the period of a year. Measurements were made at 10 time intervals during the observation period. Methodology: This was a quantitative and qualitative study. For the quantitative part, a total of 60 specimens were tested, 20 specimens for each material. The 20 specimens were further divided into 10 specimens. Ten were immersed in acidic media and the rest in saliva media. A measurement of the weight, height, and Ra was carried out as follows: day 0, day 1, day 2, day 7, day 21, day 28, day 60, day 90, day 180 and day 365. Scanning electron microscopy (SEM) was used to examine the surface of each material qualitatively pre and post immersion in the two media. For fluoride measurements, an additional five samples from each material were left suspended in the de-ionized water by the use of dental floss. The materials were moved to new specimen jars after the completion of day 1, 2, 3, 4, 5, 6, 7, 14, 21 and 28. All the specimen jars had been kept for the fluoride measurements. Results: Non-parametric tests were used to analyze the data. Linear regression analysis was used to measure the association between weight, height or surface roughness (Ra) and immersion time for a year. The result of this test showed that Vitremer had a significant association between the weight (p = 0.000), height (p = 0.007) and Ra (p = 0.001) when it was immersed in acidic media. On the other hand, when Vitremer was immersed in saliva media, only the weight variable showed a significant association (p = 0.002). For Cention N, significant association was found for only Ra when immersed in acidic media (p = 0.000). Finally, for Activa, all the studied associations; the weight, height and Ra in both media were found to be insignificant. For saliva media, there was a significant weight change between the three materials during all 10 periods of time (p = 0.000). In the first six months, Cention N demonstrated a significant increase in weight changes followed by Vitremer, then Activa. Yet, after a year, the difference between Cention N and Vitremer became insignificant and Activa showed the least weight changes. There was not a significant difference between the materials in terms of height and Ra measurements. The fluoride experiment was not successful due to technical issues during pH measurements of the collected solutions. For comparison of the studied parameters between the three materials, the Kruskal-Wallis test was used. In acidic media, there was a significant difference between the materials in term of weight change in 10 periods of time (p = 0.000). In particular, after a two month period, Cention N had the highest weight, followed by Vitremer and then by Activa. The difference between Vitremer and Activa became insignificant throughout the rest of the experimental time frame. All the height measurements between the three materials were found to be insignificant except for day 365 (p = 0.048), where both Activa and Cention N were found to be significantly higher than Vitremer. For the Ra comparison, in the first two weeks, particularly day 1, 7 and 14, Cention N had significantly the lowest Ra among the other materials. As the three materials aged in the acidic media (day 180), Vitremer had significantly the highest Ra values. Cention N showed higher Ra values than Activa; nonetheless this difference was not significant. The SEM images showed loss of some particles in all post-experimental images of the materials in acidic media. Vitremer showed the widest cracks with the loss of fillers. In saliva media, there was also loss of particles but to a lesser extent than in acidic media. Yet, the post-experimental image of Activa in saliva resembled the pre-experimental one. Conclusion: Within the limitations of the study, the best material to resist Ra from prolonged acidic attack was Activa followed by Cention N and then Vitremer. Except for Vitremer, no significant changes in the Ra of the other materials were detected when the three materials were immersed in saliva media in the long term. In acidic media Vitremer tended to lose weight and height faster than Cention N and Activa over a year. Cention N is the best material to resist dimensional change. However, in artificial saliva Vitremer gained water rapidly. Activa did not absorb a lot of water and did not reject a lot of water; Activa demonstrated good dimensional stability and this property may be beneficial when compared to the other two materials tested. The clinical significance of the study: All the materials studied were subjected to dimensional and Ra changes following long-term exposure to acidic substances, but the newer materials (Cention N and Activa) seemed to be more dimensionally stable and resistant to Ra changes than the older, well-known material (Vitremer). This may influence a clinician’s choice of restorative material for use in pediatric dentistry.
Mohammed, Khair Ro'aa Mohammed Jafar Mohammed. "Comparative in vitro study of selected physical properties of activa, cention n and vitremer." University of the Western Cape, 2021. http://hdl.handle.net/11394/8138.
Повний текст джерелаThis study aimed to determine the association between dimensional change and surface roughness (Ra) of Vitremer, Activa and Cention N after immersing them into two different media: acidic and artificial saliva media for the period of a year. Measurements were made at 10 time intervals during the observation period.
Wang, Hezhong. "Chitosan-Cellulose Nanocrystal Polyelectrolyte Complex Particles: Preparation, Characterization, and In Vitro Drug Release Properties." Diss., Virginia Tech, 2009. http://hdl.handle.net/10919/40353.
Повний текст джерелаPh. D.
Romano, Giovanni. "Micromanipulation techniques used to study in vitro DNA properties in a tethered bead configuration." Université Louis Pasteur (Strasbourg) (1971-2008), 2003. http://www.theses.fr/2003STR13228.
Повний текст джерелаKöndgen, Harold. "The dynamical response properties of neocortical neurons to temporally modulated noisy inputs "in vitro" /." Bern : [s.n.], 2008. http://www.zb.unibe.ch/download/eldiss/09koendgen_h.pdf.
Повний текст джерелаZhang, Jinya. "Unmasking the microbicidal and immunostimulatory properties of warthog fecal microbiota in vitro and in vivo." Doctoral thesis, Universitat Autònoma de Barcelona, 2020. http://hdl.handle.net/10803/670999.
Повний текст джерелаEl Virus de la Peste Porcina Africana (VPPA) es la amenaza principal para la industria porcina. La ausencia de vacunas frente al virus complica el control y erradicación de la enfermedad que este virus provoca: la Peste Porcina Africana (PPA). El VPPA infecta tanto a cerdos domésticos como a jabalíes euroasiáticos (ambos Sus Scrofa), cursando con distintos cuadros clínicos, que varían desde la PPA aguda o hiperaguda, con tasas de mortalidad de hasta el 100%, hasta la infección crónica. A pesar el VPPA es capaz de infectar también a los cerdos salvajes africanos, incluyendo al facóquero, el virus no provoca signos clínicos aparentes, convirtiéndose de hecho en reservorios naturales del VPPA. Esta Tesis se ha sustentado en dos observaciones realizadas en nuestro laboratorio. Por un lado, la infección de cerdos libres de patógenos específicos (SPF) con una cepa atenuada de VPPA, provoca un cuadro agudo de PPA, mientras que esa misma infección resultaba inocua para los cerdos domésticos convencionales. Por otro lado, estudios comparativos de especies de cerdos con muy distinta susceptibilidad a la VPPA permitieron demostrar que la composición de su microbiota intestinal, venía marcada por factores genéticos como ambientales. Estos resultados, junto con el conocimiento sobre el papel clave de la microbiota intestinal en el mantenimiento del equilibrio homeostático del organismo, la maduración del sistema inmunológico y la resistencia a los patógenos, nos condujo a plantear como objetivo general de esta tesis, investigar el papel potencial que podría desempeñar la microbiota fecal de facóquero en su resistencia a la PPA. Los objetivos específicos fueron: 1) Establecer un modelo de trasplante de microbiota fecal (TMF) de facóqueros en cerdos domésticos; 2) utilizar este modelo para comparar su susceptibilidad a la PPA, tras la infección experimental con cepas virulentas o atenuadas del VPPA; 3) aislar bacterias individuales de la microbiota fecal del facóquero para caracterizar la capacidad inmunoestimulatoria y microbicida in vitro, y 4) inocular cerdos domésticos in vivo con componentes seleccionados con el objetivo de mimetizar los efectos del TMF. Los principales hallazgos fueron el TMF de facóqueros: (1) no es perjudicial para los lechones domésticos recién destetados, (2) modifica la composición de la microbiota del receptor, (3) mejora la inmunidad de mucosas del cerdo doméstico trasplantado. (4) y confiere protección parcial contra una cepa atenuada del VPPA, mostrando una reducción muy significativa del virus en suero, excreción nasal y signos clínicos. Sin embargo, el TFM de facóquero no resultó eficiente frente la infección intramuscular con una cepa altamente virulenta del VPPA. (5) El aislamiento de bacterias a partir de heces de facóquero permitió la caracterización de componentes individuales de su microbiota in vitro. Se caracterizaron bacterias de facóquero con propiedades beneficiosas sobre el crecimiento in vitro de organoides del íleon y colon del cerdo. Se caracterizó un grupo de bacterias capaces de inhibir el crecimiento in vitro de bacterias patógenas del cerdo: Clostridium perfringens, Salmonella enterica, Salmonella entericaTyphimurium, Escherichia coli K88 y Streptococcus suis (cepas virulentas y apatogénicas). Finalmente, se caracterizó un aislado capaz de estimular la secreción in vitro de IFNγ de células del tejido linfoides asociados al intestino. (6) La inoculación intragástrica de 15 cepas seleccionadas, mejoró la inmunidad de mucosas en los animales receptores indicada por un aumento en la producción total de IgA en el suero y de la IgA específica frente al VPPA. Estos resultados han abierto una nueva vía de investigación en la búsqueda de estrategias que permitan luchar no sólo contra el VPPA, sino también contra otros patógenos del porcino. El desenmascaramiento del papel biológico de la microbiota intestinal de los facóqueros podría suponer un gran beneficio para el futuro.
African swine fever virus (ASFV) is the number one threat for the pig industry. Until today, there is no commercial vaccine or treatment available, thus complicating the control and eradication of African swine fever (ASF). ASFV can infect domestic pigs (DPs) and Eurasian wild boars (both being Sus Scrofa), resulting in different clinical disease courses, varying from acute ASF with 100 % mortality rate to chronic infection. Conversely ASFV can infect African wild pigs, including warthog, bushpig and giant forest hog, without causing apparent disease. The work here presented is based on two observations preliminary obtained in our laboratory. Firstly, specific-pathogen-free (SPF) pigs infected with attenuated ASFV strains, developed acute ASF dying in a matter of two weeks, while with same ASFV strains conventional DPs perfectly overcame the infection, despite sharing identical genetic background than the SPF pigs. This definitively demonstrated that together with genetic differences, environmental factors could also play roles in ASF susceptibility. Furthermore, fecal microbiota comparisons between two swine species (pigs and warthogs), grown in diverse environmental conditions, confirmed that microbiota composition varies depending on genetic and environmental factors. With this data at hand and taking into account that gut microbiota is one of the key players driving body homeostasis equilibrium, immune system maturation and pathogen resistance, the main objective here was to investigate the potential role of warthog fecal microbiota in ASF resistance. We proposed four specific objectives: 1) To establish a fecal microbiota transplantation (FMT) model in DPs using microbiota from DPs or warthogs. 2) To use this animal model to compare the ASF susceptibility after experimental challenge with virulent or attenuated ASFV strains. 3) To isolate individual bacteria from warthog fecal microbiota for further characterization of their in vitro microbicidal or immunostimulatory capabilities, and 4) to inoculate in vivo DPs with selected components of the in vitro characterized microbiota, aiming to mimic the effects observed after FMT. The main findings obtained can be summarized as follows: (1) Transplantation of fecal microbiota from warthog is not harmful to domestic weaned piglets. (2) FMT from warthog modifies the microbiota composition of transplanted DPs. (3) FMT from warthog improves the mucosal immunity of transplanted DPs, with higher levels of total IgA in sera. (4) FMT from warthog to DPs confers partial protection against intramuscular infection with E75CV1, an attenuated strain. Thus, pigs transplanted with fecal microbiota from warthog, showed a very significant reduction of virus in serum, nasal shedding and clinical signs, while FMT from pigs to pigs did not. No effect was observed against intramuscular challenge with E75, a highly virulent strain. (5) Isolation of individual bacteria from warthog feces allowed the characterization of individual microbiota components in vitro. Therefore, some bacteria showed beneficial properties on pig ileum and colon organoids, others showed microbicidal properties against different pig pathogenic bacteria, including: Clostridium perfringens (type B), Salmonella enterica, Salmonella enterica serovar Typhimurium monophasic variant, Escherichia coli K88 and Streptococcus suis (virulent and apathogenic strains). Several bacteria were able to stimulate in vitro IFNγ secretion by gut associated lymphoid tissues, a key cytokine involved in ASFV protection. (6) The intragastric inoculation of 15 selected isolates strains, improved the mucosal immunity in the recipient animals denoted by the increase in total IgA production in sera and the ASFV-specific IgA in both serum and nasal swabs upon E75CV1 challenge. The results obtained during the present doctoral thesis will open new avenues for the future fighting not only against ASFV, but also against other pathogens.”
Murray, Hilary Elaine. "An investigation of the morphological and functional properties of rat hypothalamic dopaminergic systems in vitro." Thesis, Imperial College London, 1994. http://hdl.handle.net/10044/1/37113.
Повний текст джерелаBerger, Gilles. "Synthesis of chiral vicinal diamines and in vitro anticancer properties of their platinum(II) coordinates." Doctoral thesis, Universite Libre de Bruxelles, 2013. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209376.
Повний текст джерелаInfrared spectroscopy probes the global chemical composition of a sample and has been used to produce a snapshot of cancer cells contents after treatment with platinum coordinates. Indeed, in vitro studies focused here on the use of modern spectroscopic methods to fingerprint the cellular impact of platinum complexes. These drug signatures help to classify and select promising compounds. It makes no doubt that such systemic approaches for compound discovery are helpful technologies. Also, we made the use of the COMPARE algorithm from the NCI, which analyzes similarity between any active compounds previously tested by the NCI large scale in vitro screening program of anticancer agents.
The last chapter aimed to study the interactions between a series of platinum coordinates and DNA. Binding mode to telomeric-like sequences and binding kinetics to genomic-like sequences were assessed to investigate any differences between the compounds and to gain insight into structure-activity relationships.
Doctorat en Sciences biomédicales et pharmaceutiques
info:eu-repo/semantics/nonPublished
Westerlund, Ulf. "Adult human neural stem cells : properties in vitro and as xenografts in the spinal cord /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-412-0/.
Повний текст джерелаLnenicek-Allen, Mirna. "Studies of the DNA binding and bending properties of HMG boxes using in vitro mutagenesis." Thesis, University of Portsmouth, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310138.
Повний текст джерелаDempsey, Claire. "An investigation into the phenotype, function and immunomodulatory properties of in vitro expanded iNKT cells." Thesis, University of Birmingham, 2019. http://etheses.bham.ac.uk//id/eprint/8843/.
Повний текст джерелаRitchie, Jennifer Lynn. "The Material Properties of the Chordae Tendineae of the Mitral Valve: An In Vitro Investigation." Thesis, Available online, Georgia Institute of Technology, 2004:, 2004. http://etd.gatech.edu/theses/available/etd-09062004-120502/unrestricted/Ritchie%5FJennifer%5FL%5F200412%5FMS.pdf.
Повний текст джерелаSacks, Michael, Committee Member ; Vito, Raymond, Committee Member ; Guldberg, Robert, Committee Member ; Yoganathan, Ajit, Committee Chair. Includes bibliographical references.
Daneshian, Mardas. "Detection and characterization of the detection and characterization of the immunostimulatory properties of airborne pyrogens." [S.l. : s.n.], 2006. http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-23677.
Повний текст джерела