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Статті в журналах з теми "Immunologie humaine"

Автор: Grafiati

Опубліковано: 13 квітня 2024

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1

Benkirane, M. "Topographie immunologique de la TSH humaine : applications aux dosages immunométriques." Immuno-analyse & Biologie Spécialisée 4, no. 6 (December 1989): 17–22. http://dx.doi.org/10.1016/s0923-2532(89)80003-1.

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2

Dirnhofer and Berger. "Molekulare Struktur des humanen Choriongonadotropin: Neue Funktionen in der Immunologie, Endokrinologie und Onkologie." Praxis 91, no. 19 (May 1, 2002): 845–48. http://dx.doi.org/10.1024/0369-8394.91.19.845.

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Das humane Choriongonadotropin (hCG) ist das klassische Schwangerschaftshormon und darüber hinaus ein etablierter Tumormarker für trophoblastäre Geschwülste und Hodentumoren. Es wird aber physiologischerweise auch in nicht schwangeren Normalpersonen von der Hypophyse und dem Hoden gebildet und ist zusätzlich ein Tumormarker für das Urothelkarzinom. Dies weist auf bisher wenig untersuchte auto-/parakrine Wirkungen dieses Hormons in der Endokrinologie und der Onkologie hin. Aufgrund dieser fehlenden Schwangerschaftsspezifität sowie immunologischer Ähnlichkeiten mit anderen Glykoproteinhormonen und letztlich fehlender Wirksamkeit ist das hCG kein geeignetes Antigen für eine fertilitätsregulierende Impfung.

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3

Benkirane, M., M. Cordeil, P. Princé, F. Moreux, J. L. Drocourt та M. Delaage. "Du dosage immunoradiométrique au dosage radio-immunologique. Evolution d'un dosage de β2 microglobuline humaine". Immuno-analyse & Biologie Spécialisée 5, № 2 (квітень 1990): 75–79. http://dx.doi.org/10.1016/s0923-2532(05)80204-2.

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4

Chassouros, Alexandre, Alexandre Essakhi, Anis Khiat, Shirihane Kouadri, Aïda Tadjine, Nadia Tadjine, Pol Ubeda, and Victorine Zhang. "L’actualité immunologique sous l’oeil critique des étudiants de Master 2." médecine/sciences 34, no. 3 (March 2018): 219–22. http://dx.doi.org/10.1051/medsci/20183403009.

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Анотація:

Pour la quatrième année consécutive, les étudiants du Master 2 « Immunothérapies et bio-ingénierie » de l’Université Pierre et Marie Curie (Sorbonne Université) ont pris la plume pour partager avec les lecteurs de m/s quelques-uns des faits marquants de la littérature de fin 2017 dans le domaine de l’immunologie. Cette année, ils nous rappellent combien l’immunothérapie est porteuse d’espoir en cancérologie en relatant deux approches très novatrices : l’une, indirecte, utilise des nanovésicules bactériennes, l’autre réalise une vaccination antitumorale fondée sur des néoantigènes tumoraux spécifiquement identifiés chez les patients. Deux autres brèves rapportent les résultats d’études dont l’impact clinique est évident : l’une identifie un anticorps thérapeutique efficace et sûr contre le virus Zika, l’autre décrypte le lien entre immunosuppression exercée par les Treg et autophagie dans les cellules dendritiques, et offre une explication à l’activité thérapeutique d’un activateur de la voie CTLA4/B7. Toutes ces découvertes ont une application directe en clinique humaine, ce qui souligne, une fois encore, combien la recherche est essentielle au progrès médical ! Bonne lecture ! Sophie Sibéril (Maître de conférence Sorbonne Université, responsable du module « analyse scientifique » du M2 immunothérapies et bio-ingénierie)

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5

Hauck, Fabian. "Angeborene autoinflammatorische Störungen der cGAS-STING- und OASRNase-L-vermittelten Nukleinsäure-Immunität." Translationale Immunologie 5, no. 3 (October 28, 2021): 160–65. http://dx.doi.org/10.47184/ti.2021.03.04.

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Typ-I-Interferonopathien sind autoinflammatorische Störungen der angeborenen Nukleinsäure-Immunität, die zusätzlich zu Epitop-spezifischer Autoimmunität prädispositionieren. In der Immunbiologie ist die Nukleinsäure-Immunität vermittelt durch die cGAS-STING- und OAS-RNase-L-Signalwege weitgehend verstanden. In der translationellen und klinischen Immunologie zeigen angeborene Störungen dieser Signalwege deren physiologische Relevanz an der Schnittstelle zwischen Selbst- und Fremd-Nukleinsäure-Erkennung und führen zu einem neuen Verständnis von seit Langem bekannten Krankheitsbildern. Dieser Übersichtsartikel fasst aktuelle pathophysiologische Konzepte und deren klinische Implikationen zur unkontrollierten cGAS- und OAS1-Aktivierung durch biallelische LSM11 und RNU7-1-loss-of-function-, monoallelische ATAD3A dominant-negative und monoallelische OAS1-gain-of-function-Varianten zusammen. Eine murine monoallelische dominante Oas2-Variante wird als Modell für eine mögliche Organ-spezifische humane Typ-I-Interferonopathie diskutiert.

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6

Khau, Sandra, and Cassandra Lopatynski. "Les génomes viraux défectueux du virus Chikungunya: Vers une nouvelle approche d’antiviraux à large spectre ?" médecine/sciences 38, no. 11 (November 2022): 955–59. http://dx.doi.org/10.1051/medsci/2022141.

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Анотація:

Le Master 2 « Infectiologie, Immunologie, Vaccinologie et Biomédicaments (I2VB) », dispensé à la faculté de Pharmacie de l’université de Tours, propose de donner les bases conceptuelles et pratiques des différents aspects de l’infectiologie et de l’immunologie dans un contexte d’innovation thérapeutique. Il s’appuie sur une coopération exemplaire entre les équipes de recherche en infectiologie et en immunologie de l’université de Tours, et celles, entre autres, de l’unité « Infectiologie et Santé Publique » (ISP) et de l’unité « Physiologie de la Reproduction et des Comportements » (PRC) du Centre INRAE de Tours-Nouzilly, concrétisée par une profonde interaction entre chercheurs et enseignants-chercheurs. Cette formation aborde aussi bien les aspects fondamentaux et appliqués de l’infectiologie et de l’immunologie, allant de l’étude moléculaire des interactions entre le pathogène et son hôte, jusqu’à la conception et la mise sur le marché des produits de la vaccinologie, des biothérapies anti-infectieuses et des anticorps thérapeutiques. Le Master 2 I2VB a pour objectif de former : – de jeunes scientifiques aux enjeux actuels de l’infectiologie et des biomédicaments tels que les anticorps. – des experts pour gérer les risques d’émergences, et capables de comprendre les interactions complexes entre un agent infectieux et son hôte humain ou animal, capables de proposer des mesures préventives ou des thérapies innovantes.

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7

Mairesse, M., F. Debaugnies, V. Doyen, C. Ledent, O. Michel, F. Corazza, and F. Francis. "Risque allergique des insectes en alimentation humaine." Revue Française d'Allergologie 54, no. 3 (April 2014): 228. http://dx.doi.org/10.1016/j.reval.2014.02.009.

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8

Brandtner, Anna, Mirjam Bachler, Dietmar Fries, Martin Hermann, Jacqueline Ruehlicke, Vilmos Fux, Andrea Griesmacher, Christian Niederwanger, Tobias Hell, and Benedikt Treml. "Tigecycline Interferes with Fibrinogen Polymerization Independent of Peripheral Interactions with the Coagulation System." Antibiotics 9, no. 2 (February 14, 2020): 84. http://dx.doi.org/10.3390/antibiotics9020084.

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Tigecycline offers broad anti-bacterial coverage for critically ill patients with complicated infections. A described but less researched side effect is coagulopathy. The aim of this study was to test whether tigecycline interferes with fibrinogen polymerization by peripheral interactions. To study the effect of unmetabolized tigecycline, plasma of healthy volunteers were spiked with increasing concentrations of tigecycline. In a second experimental leg, immortalized human liver cells (HepG2) were treated with the same concentrations to test an inhibitory effect of hepatic tigecycline metabolites. Using standard coagulation tests, only the activated thromboplastin time in humane plasma was prolonged with increasing concentrations of tigecycline. Visualization of the fibrin network using confocal live microscopy demonstrated a qualitative difference in tigecycline treated experiments. Thrombelastometry and standard coagulation tests did not indicate an impairment of coagulation. Although the discrepancy between functional and immunologic fibrinogen levels increased in cell culture assays with tigecycline concentration, fibrinogen levels in spiked plasma samples did not show significant differences determined by functional versus immunologic methods. In our in vitro study, we excluded a direct effect of tigecycline in increasing concentrations on blood coagulation in healthy adults. Furthermore, we demonstrated a rapid loss of mitochondrial activity in hepatic cells with supra-therapeutic tigecycline dosages.

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9

Lux, Sebastian. "EAA oder humane Psittakose?" Allergo Journal 27, no. 4 (June 2018): 8. http://dx.doi.org/10.1007/s15007-018-1605-z.

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10

Nicolas, J. C., C. Kaplan, A. M. Couroucé, F. Bricout, and J. J. Lefrère. "Quelles sérologies virales demanderdans le purpura thrombopénique immunologique chez le sujet non infecté par le virus de l'immunodéficience humaine?" La Revue de Médecine Interne 10, no. 1 (January 1989): 78–79. http://dx.doi.org/10.1016/s0248-8663(89)80124-9.

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11

Coudeyras, Sophie, and Christiane Forestier. "Microbiote et probiotiques : impact en santé humaine." Canadian Journal of Microbiology 56, no. 8 (August 2010): 611–50. http://dx.doi.org/10.1139/w10-052.

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Анотація:

All accessible mucous membranes of the human body are colonized by an abundant and diversified microbial flora called microbiota. Recent studies have shown that these microorganisms, long regarded as purely commensal, have essential beneficial effects on human health. Thus, numerous human ailments are linked to dysbiosis; that is, imbalances in the microflora composition. The administration of probiotic microorganisms could, in some situations, provide substantial relief from such disorders. These live microorganisms, which, according to the definition, confer a health benefit to the host when administered in adequate amounts, are often derived from human flora and belong mostly to lactic acid bacteria, in particular to the genus Lactobacillus . The constant improvement of knowledge of the role of human microbiota and the growing popularity of probiotics are now opening the door to new prophylactic and therapeutic strategies in human health.

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12

Van der Brempt, X., and D. A. Moneret-Vautrin. "Le risque allergique de Tenebrio molitor pour la consommation humaine." Revue Française d'Allergologie 54, no. 1 (January 2014): 34–36. http://dx.doi.org/10.1016/j.reval.2013.09.006.

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13

Colas, L., G. Veyrac, A. Pipet, G. Gaillet, C. Bernier, and A. Magnan. "Une bien étrange hypersensibilité aux insulines humaines…" Revue Française d'Allergologie 59, no. 3 (April 2019): 276. http://dx.doi.org/10.1016/j.reval.2019.02.109.

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14

Anelli, Alberto. "La réduction phénoménologique de Husserl et le problème de la liberté humaine." Transversalités 164, no. 1 (January 16, 2023): 103–13. http://dx.doi.org/10.3917/trans.164.0103.

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15

Pouradier, Maud. "L’idée d’université et la place des humanités et sciences humaines : état des lieux." Transversalités 167, no. 4 (October 9, 2023): 69–80. http://dx.doi.org/10.3917/trans.167.0069.

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Анотація:

Une université n’est pas seulement une école de formation supérieure : son idée implique une recherche de la connaissance pour elle-même, et la préservation de la culture. L’état des humanités et sciences humaines au sein de l’institution universitaire permet de voir si l’idée d’université y est encore vivace. Or ces disciplines sont concurrencées par des domaines d’études nouveaux, comme les sciences de l’éducation ou les études de genre, qui menacent directement le maintien des formations classiques. Les ingénieurs pédagogiques, nouveaux venus dans le paysage universitaire depuis le COVID, mènent une guerre contre le cours magistral, lequel est la forme normale d’enseignement dans les humanités et sciences humaines. Heureusement, l’idée d’université reste dans le cœur de maints universitaires, parfois contre l’institution elle-même.

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16

Daëron, M. "Les récepteurs pour les anticorps des basophiles humains." Revue Française d'Allergologie 56, no. 3 (April 2016): 114–16. http://dx.doi.org/10.1016/j.reval.2016.01.038.

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17

Kamala, T. "Hock immunization: A humane alternative to mouse footpad injections." Journal of Immunological Methods 328, no. 1-2 (December 2007): 204–14. http://dx.doi.org/10.1016/j.jim.2007.08.004.

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18

Nick Ishmael-Perkins. "The future is bright, humane, and uneven for chemistry." C&EN Global Enterprise 102, no. 9 (March 25, 2024): 2. http://dx.doi.org/10.1021/cen-10209-editorial.

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19

Devouassoux, G. "Le basophile humain et la réponse immunitaire." Revue Française d'Allergologie et d'Immunologie Clinique 44, no. 1 (January 2004): 1–8. http://dx.doi.org/10.1016/j.allerg.2003.10.007.

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20

C.P. "L'IL-4 induit l'apoptose des éosinophiles sanguins humains." Revue Française d'Allergologie et d'Immunologie Clinique 40, no. 6 (October 2000): 660–61. http://dx.doi.org/10.1016/s0335-7457(00)80148-3.

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21

Thurman, Colleen E., Skye Rasmussen, and Kevin A. Prestia. "Effect of 3 Euthanasia Methods on Serum Yield and Serum Cortisol Concentration in Zebrafish (Danio rerio)." Journal of the American Association for Laboratory Animal Science 58, no. 6 (November 1, 2019): 823–28. http://dx.doi.org/10.30802/aalas-jaalas-18-000144.

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Анотація:

Zebrafish are an important model in neuroscience and developmental biology and are also an emerging model in hematology and immunology. Little information is available for zebrafish regarding the physiologic impact of different euthanasia methods and whether a chosen method of euthanasia can impact serum yield. These parameters could impact the choice of euthanasia method for a study. To that end, the current study compared 3 methods of adult zebrafish euthanasia and their effects on 3 distinct criteria; time to loss of opercular movement, volume of serum obtained, and serum cortisol concentration. Blood was collected using a postmortem tail amputation and centrifugation blood collection technique. Time to loss of opercular movement differed significantly among euthanasia methods, with animals undergoing rapid chilling displaying the shortest time (mean Rapid Chilling: 40 s; Benzocaine: 86 s; MS222: 96 s). All methods of euthanasia resulted in a comparable average serum yield (Rapid Chilling = 7.5 μL; Benzocaine = 8.5 μL; MS222 = 7.5 μL per fish). None of the euthanasia methods tested resulted in average cortisol concentrations above the reported physiologic range. Although no significant differences were observed in serum yield or serum cortisol concentration, rapid chilling remains the preferred method for painless, humane euthanasia.

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22

Chi Hieu, C., N. Van Dinh, C. Vidal, R. B. Fulton, N. Nhu Nguyet, D. Thi Quynhnga, T. Thi Linh Tu, et al. "Réaction cutanée sévère associées aux antigènes leucocytes humains chez les Vietnamiens." Revue Française d'Allergologie 59, no. 3 (April 2019): 276–77. http://dx.doi.org/10.1016/j.reval.2019.02.110.

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23

Leynadier, F., J. Dry, A. Aidaoui, and M. Khellaf. "Dégranulation des basophiles humains après intervention dans l'hydatidose pulmonaire." Revue Française d'Allergologie et d'Immunologie Clinique 25, no. 1 (January 1985): 42. http://dx.doi.org/10.1016/s0335-7457(85)80032-0.

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24

Hilfenhaus, J. "HIV-Sicherheit industriell hergestellter humaner Plasmaproteine." Transfusion Medicine and Hemotherapy 21, no. 1 (1994): 84–88. http://dx.doi.org/10.1159/000223045.

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25

Gupta, P. D., and Alpana Gupta. "The Immuno-Pathology of the Human Placenta." Obstetrics Gynecology and Reproductive Sciences 5, no. 2 (March 20, 2021): 01–03. http://dx.doi.org/10.31579/2578-8965/0060.

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Анотація:

The human placenta once was thrown after the delivery, found to be a very useful organ though it has very short life. With more and new research the old concept about placenta has changed. Now it is well established that health of growing embryo depends on the health of the placenta. To begin with immunology of neonate also depends on transplacental transport. It is well established that the health of the growing embryo depends on the health of the transplacental transport. Normally, IgG can be transported through placenta however, in Covid-19 infected pregnant woman even IgM, which is much bigger molecule than IgG, can also be transported and are found in the embryo.

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26

Karachaliou, Chrysoula-Evangelia, and Evangelia Livaniou. "Neuroprotective Action of Humanin and Humanin Analogues: Research Findings and Perspectives." Biology 12, no. 12 (December 16, 2023): 1534. http://dx.doi.org/10.3390/biology12121534.

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Анотація:

Humanin is a 24-mer peptide first reported in the early 2000s as a new neuroprotective/cytoprotective factor rescuing neuronal cells from death induced by various Alzheimer’s disease-associated insults. Nowadays it is known that humanin belongs to the novel class of the so-called mitochondrial-derived peptides (which are encoded by mitochondrial DNA) and has been shown to exert beneficial cytoprotective effects in a series of in vitro and/or in vivo experimental models of human diseases, including not only neurodegenerative disorders but other human diseases as well (e.g., age-related macular degeneration, cardiovascular diseases, or diabetes mellitus). This review article is focused on the presentation of recent in vitro and in vivo research results associated with the neuroprotective action of humanin as well as of various, mainly synthetic, analogues of the peptide; moreover, the main mode(s)/mechanism(s) through which humanin and humanin analogues may exert in vitro and in vivo regarding neuroprotection have been reported. The prospects of humanin and humanin analogues to be further investigated in the frame of future research endeavors against neurodegenerative/neural diseases have also been briefly discussed.

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27

Filonenko, Lesya, Iryna Demchenko, Iryna Shakhina, Oleksandr Klochok, Теtiana Borozentseva, and Vitalii Bieloliptsev. "Developing a Values-Based Attitude towards People in Adolescent Rehabilitation Centres: Neurophysiological Patterns." BRAIN. Broad Research in Artificial Intelligence and Neuroscience 13, no. 2 (June 30, 2022): 76–96. http://dx.doi.org/10.18662/brain/13.2/333.

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The article theoretically justifies, defines and experimentally verifies pedagogical conditions for developing a values-based attitude towards people in adolescents from rehabilitation centres, taking into account pedagogical, psychological and neurophysiological factors. According to the above-mentioned factors, such pedagogical conditions can be developed in the context of the following aspects: involving adolescents from rehabilitation centres in humane activity in terms of developing a values-based attitude towards people; making the “teacher-pupil” and “pupil-pupil” relationships more humanistic; increasing the readiness of rehabilitation centres teachers to develop a values-based attitude towards people in adolescents. Research methods are as follows: surveys, conversations, interviews; writing a mini-essay on the topic “Another Person in My Life”; the questionnaire on values-based orientations; the verbal associations method; discussions, tests, self-tests (assessing one’s behaviour in a conflict situation); the “eye to eye” adapted methodology; the story-role game “One Family”; pedagogical observations; an analysis of life and specially modelled situations. The international relevance of the article lies in the following: for the first time, pedagogical conditions for developing a values-based attitude towards other people in adolescents from rehabilitation institutions in Ukraine have been justified (elaborating and introducing the content, forms and methods; involving adolescents in humane activity; humanizing the relations in the teacher-pupil and pupil-pupil systems; enhancing the level of teachers’ readiness to develop a values-based attitude towards people in adolescents); relevant criteria with corresponding indicators have been determined. This can serve as an impetus to further research on the problem in question on the example of developing countries.

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28

Imiela, A., J. Y. Tavernier, I. Carrotte-Lefebvre, F. Devemy, E. Delaporte, and C. Lamblin. "Allergie à l’insuline humaine recombinante : à propos de 3 cas avec manifestations immédiates généralisées." Revue Française d'Allergologie et d'Immunologie Clinique 43, no. 3 (April 2003): 165–69. http://dx.doi.org/10.1016/s0335-7457(03)00045-5.

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29

Berger, P., J. M. Tunon-de-Lara, and R. Marthan. "PAR-2 et activation des cellules musculaires lisses bronchiques humaines." Revue Française d'Allergologie et d'Immunologie Clinique 44, no. 3 (April 2004): 251–53. http://dx.doi.org/10.1016/j.allerg.2004.01.005.

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30

Damolga, M., and Et Al. "Facteurs associés aux discordances immunovirologiques, aux échecs immunologiques et virologiques chez les patients vivant avec le VIH-1 et suivis à l'hôpital de jour de Bobo-Dioulasso en 2019." Revue Malienne d'Infectiologie et de Microbiologie 18, no. 1 (June 23, 2023): 16–21. http://dx.doi.org/10.53597/remim.v18i1.2632.

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Анотація:

Objectif: Déterminer la prévalence des discordances immunovirologiques, des échecs immunologiques et virologiques et les facteurs associés chez les patients vivant avec le VIH-1 et suivis à l'hôpital de jour de Bobo-Dioulasso. Méthodes: Cette étude transversale descriptive a été menée de janvier à décembre 2019, incluant les patients séropositifs pour le virus de l'immunodéficience humaine VIH-1 suivis à l'hôpital de jour pour adultes de Bobo-Dioulasso et, ayant bénéficié de deux mesures consécutives de la charge virale et du taux de lymphocytes T CD4 au cours de l'année. Les données extraites de la base de données d'évaluation et de suivi opérationnel des programmes ESTHER ont été analysées à l'aide de STATA et SPSS V.20.0Résultats : Un total de 261 patients a été inclus dans cette étude. L'âge médian de la population étudiée était de 46 ± 10,64 ans, avec une prédominance féminine (70,50%). Nous avons noté que 8 patients étaient en échec immunologique (3,06%) avec une prédominance des personnes mariées et des sujets âgés de 50-59 ans. 115 patients étaient en échec virologique (44,06%) avec une majorité de femmes. La prévalence de la discordance immunovirologique était de (1,92%) avec une prédominance masculine (60%). Parmi les patients présentant une discordance immunovirologique, 60 % étaient âgés de 40 à 49 ans et 60 % étaient mariés. Conclusion : La prévalence des échecs virologiques était de 44,06% dans notre population étudiée, celles des discordances immunovirologiques et des échecs immunologiques étaient faibles, soit respectivement 1,92% et 3,06%. Les principaux groupes rencontrés dans les discordances immunovirologiques et les échecs immunologiques et virologiques dans notre population d'étude étaient la tranche d'âge 40-59 ans, les femmes de ménage, les personnes mariées et non scolarisées

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31

Schallehn, Gisela, and Manfred Helmut Wolff. "Morphologische Veränderungen humaner embryonaler Lungenfibroblasten durch cytotoxine verschiedener clostridium-spezies." Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology 267, no. 3 (January 1988): 367–78. http://dx.doi.org/10.1016/s0176-6724(88)80053-1.

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Aebischer, Iwan, Martin R. Stämpfli, Adrian Zürcher, Sylvia Miescher, Adrian Urwyler, Brigitte Freyu, Thomas Lugeru, Richard R. Whiteu, and Beda M. Stadler. "Neuropeptides are potent modulators of humanin vitro immunoglobulin E synthesis." European Journal of Immunology 24, no. 8 (August 1994): 1908–13. http://dx.doi.org/10.1002/eji.1830240829.

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33

Elbim, C., S. Chollet-Martin, S. Bailly, J. Hakim, and M. A. Gougerot-Pocidalo. "Régulation par les cytokines de l'explosion oxydative des polynucléaires neutrophiles humains." Revue Française d'Allergologie et d'Immunologie Clinique 34, no. 6 (December 1994): 489–90. http://dx.doi.org/10.1016/s0335-7457(05)80394-6.

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34

Martin, M. P., R. M. Single, M. J. Wilson, J. Trowsdale, and M. Carrington. "KIR haplotypes defined by segregation analysis in 59 Centre d’Etude Polymorphisme Humain (CEPH) families." Immunogenetics 60, no. 12 (October 30, 2008): 767–74. http://dx.doi.org/10.1007/s00251-008-0334-y.

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35

Martin, M. P., R. M. Single, M. J. Wilson, J. Trowsdale, and M. Carrington. "KIR haplotypes defined by segregation analysis in 59 Centre d’Etude Polymorphisme Humain (CEPH) families." Immunogenetics 61, no. 1 (December 9, 2008): 79. http://dx.doi.org/10.1007/s00251-008-0345-8.

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Dion, Michel, and Claude Hamelin. "Cartographie physique de l'ADN du cytomégalovirus humain souche AD169." Canadian Journal of Microbiology 36, no. 5 (May 1, 1990): 341–47. http://dx.doi.org/10.1139/m90-059.

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The whole human cytomegalovirus strain AD169 genome was cloned into plasmid pAT153 in the form of 25 HindIII fragments. Double and triple digestions of the recombinant plasmids with restriction endonucleases BamHI, BglII, ClaI, DraI, EcoRI, EcoRV, HindIII, HpaI, KpnI, PaeR7, PstI, SphI and XbaI yielded a detailed restriction map of human cytomegalovirus DNA. Knowing the exact position of numerous restriction sites in the viral DNA molecule, we have been able to examine very closely the heterologous region between the long and the short segments of the human cytomegalovirus genome. Key words: DNA, physical map, cytomegalovirus, restriction endonucleases, HCMV.

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37

Pactat, Inès. "Le recyclage du verre au temps des Romains, des Francs et des Byzantins." Reflets de la physique, no. 74 (December 2022): 16–21. http://dx.doi.org/10.1051/refdp/202274016.

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Le recyclage du verre ne date pas de 1974, année où la première collecte en France de verres d’emballage est organisée en Haute-Marne. Si cette initiative est désormais intégrée à notre quotidien, elle n’est néanmoins qu’un jalon récent de l’histoire de cette pratique. À partir de plusieurs exemples de cargaisons antiques et byzantines chargées de verre brisé, de récits de vols de fenêtres et de vestiges archéologiques d’ateliers, nous proposons d’en retracer le cours, de l’Antiquité romaine au Moyen Âge. La physique et la chimie permettent désormais de reconnaître ces activités de recyclage à partir d’analyses de la composition élémentaire du verre, nous faisant cheminer à la croisée des sciences humaines et des sciences dures.

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38

Leynadier, F., M. Murrieta, C. Garcia-Duarte, and J. Dry. "Evolution de la dégranulation des basophiles humains après désensibilisation accélérée aux pneumallergènes." Revue Française d'Allergologie et d'Immunologie Clinique 25, no. 2 (April 1985): 106–7. http://dx.doi.org/10.1016/s0335-7457(85)80049-6.

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39

Yang, Xiao-Dong, Alain L. De Weck, and Beda M. Stadler. "induction of humanin vitro ige synthesis via stimulation by anti-cd3 antibody." European Journal of Immunology 18, no. 3 (March 1988): 467–71. http://dx.doi.org/10.1002/eji.1830180322.

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Larson, Erica C., Mark A. Rodgers, Amy L. Ellis, Cassaundra L. Ameel, Tonilynn M. Baranowski, Alexis J. Balgeman, Pauline A. Maiello, Shelby L. O’Connor, and Charles A. Scanga. "Pre-existing SIV infection decreases cytokine responses by T cells in lung during the early stages of M. tuberculosis co-infection." Journal of Immunology 202, no. 1_Supplement (May 1, 2019): 190.36. http://dx.doi.org/10.4049/jimmunol.202.supp.190.36.

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Abstract Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), is the leading cause of death among HIV+ individuals. The precise mechanisms by which HIV impairs the immune response to subsequent Mtb infection are unknown. We previously established a model of co-infection in Mauritian cynomolgus macaques (MCM). We found SIV/Mtb co-infected MCM had rapidly progressive TB and reached humane endpoint by 12 weeks post-Mtb infection. We hypothesized that pre-existing SIV impacts T cell responses at the early stages of Mtb infection. We infected MCM with SIVmac239 intrarectally and 6 months later co-infected with a low dose of Mtb. SIV-naïve MCM were infected with Mtb alone as controls. Animals were monitored by clinical parameters, culturing bacilli in gastric and bronchoalveolar lavages, and serial 18F-FDG PET/CT imaging. Six weeks after Mtb infection, animals were necropsied and immune responses in the lung were measured by flow cytometry. The two groups exhibited similar TB disease at time of necropsy. Total bacterial burden was remarkably high in both SIV-naïve and SIV/Mtb groups, although co-infected animals tended to have more bacteria in lung tissue. At sites of Mtb infection, SIV/Mtb co-infected animals had fewer CD4+ T cells and significantly more CD8+ T cells. TNFα production by these CD4+ and CD8+ T cells was decreased. Taken together, pre-existing SIV decreases the quality of cytokine responses by T cell at sites of Mtb infection during the early stages of TB disease. This appears to be a critical time point at which the immunologic defect from pre-existing SIV infection begins to impact the arc of TB disease.

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41

Islam, Md Rakibul, Jalpa Patel, Patricia Ines Back, Hilary Shmeeda, Konstantin Adamsky, Hui Yang, Carlos Alvarez, Alberto A. Gabizon, and Ninh M. La-Beck. "Abstract A42: Impact of liposomal drug delivery and alendronate co-encapsulation on the immune modulatory effects of doxorubicin in the tumor microenvironment." Cancer Immunology Research 10, no. 12_Supplement (December 1, 2022): A42. http://dx.doi.org/10.1158/2326-6074.tumimm22-a42.

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Abstract Although Doxorubicin is the standard of care in advanced soft tissue sarcomas, therapeutic efficacy is minimal. A major contributing factor appears to be an immunosuppressive tumor microenvironment (TME). We have shown that liposomal alendronate, an amino-bisphosphonate, can remodel the immunosuppressive TME towards an immune-permissive milieu. We postulate that co-encapsulating alendronate with doxorubicin in pegylated liposomes (PLAD) will increase the efficacy of doxorubicin as alendronate abrogates the activity of suppressive myeloid cells and liposomes facilitate the accumulation of drugs in tumors. PLAD was formulated with hydrogenated soybean phosphatidyl-choline, methoxy-polyethylene glycol2000-distearoyl-phosphatidylethanolamine, and cholesterol at a molar ratio of 55:5:40, with 1 mg/ml doxorubicin and 0.43 mg/ml alendronate. WEHI-164 fibrosarcoma cells were implanted subcutaneously in male balb/c mice. For assessing the tumor immunologic milieu, mice were randomized when tumor size reached ~300 mm3 to receive PLAD, pegylated liposomal doxorubicin (PLD), or free doxorubicin (FDox) at 8 mg/kg of doxorubicin (n=9/group) or vehicle (n=5) IV. Tumors were collected 5 days post-treatment for FACS analysis. To assess antitumor efficacy, mice were randomized when tumor size reached ~150 mm3 to receive PLAD, PLD, or FDox at 5 mg/kg of doxorubicin (n=9/group) or vehicle (n=5) IV weekly and euthanized at humane endpoints. PLAD, and to a lesser extent PLD, shifted cellular drug uptake to tumor-associated macrophages (TAM) and to monocytic myeloid-derived suppressor cells (mMDSC) compared to free doxorubicin which had cellular drug uptake below detectable levels. PLAD and PLD were associated with fewer total TAMs and a decreased polarization to pro-tumoral M2-TAMs compared to free doxorubicin. Only PLAD significantly increased tumor infiltration of antigen cross-presenting dendritic cells, while both PLAD and PLD significantly increased the number and functionality of NKT and NK cells that are consistent with enhanced antitumor immune responses in the TME. All treatments significantly reduced regulatory T cell infiltration which could help promote antitumor responses in the TME. Interestingly, FDox treatment induced significant changes in the TME that could diminish antitumor immune responses by decreasing M1 TAMs and NK cells. These effects are reflected in the therapeutic study which demonstrates that PLAD and PLD inhibited tumor growth and significantly prolonged survival, while free doxorubicin showed little or no anticancer activity in this advanced fibrosarcoma model. We found that liposomal delivery is required for effective drug uptake by the TAMs and mMDSCs. Co-encapsulation of alendronate and doxorubicin led to a remodeling of the TME by abrogating pro-tumoral immune responses. This strongly suggests that the PLAD formulation is a promising liposome-based drug carrier to potentiate immunotherapy in synergy with its cytotoxic effects. Citation Format: Md Rakibul Islam, Jalpa Patel, Patricia Ines Back, Hilary Shmeeda, Konstantin Adamsky, Hui Yang, Carlos Alvarez, Alberto A Gabizon, Ninh M La-Beck. Impact of liposomal drug delivery and alendronate co-encapsulation on the immune modulatory effects of doxorubicin in the tumor microenvironment [abstract]. In: Proceedings of the AACR Special Conference: Tumor Immunology and Immunotherapy; 2022 Oct 21-24; Boston, MA. Philadelphia (PA): AACR; Cancer Immunol Res 2022;10(12 Suppl):Abstract nr A42.

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Wen, Xiangshu, Seil Kim, Agnieszka Lawrenczyk, Ran Xiong, Ping Rao, Giulia Casorati, Paolo Dellabona, and Weiming Yuan. "A unique human-like CD8+ iNKT cells in a humanized mouse model (APP2P.113)." Journal of Immunology 192, no. 1_Supplement (May 1, 2014): 43.14. http://dx.doi.org/10.4049/jimmunol.192.supp.43.14.

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Abstract The distinct differences in CD1d/NKT lipid presentation systems in humans and mice largely account for the minimal success of Natural Killer T (NKT) cell-based anti-tumor clinical trials in humans, contrasting their strong anti-tumor effects in conventional mouse models. We aim to humanize mice at the molecular level to better model human CD1d/NKT lipid presentation in vivo. Previously, we reported a novel human CD1d knock-in (hCD1d-KI) mouse, which contain a human-like abundance of iNKT cells yet possess potent anti-tumor immunity by the iNKT cells (Proc. Natl. Acad. Aci. USA 110: 2963-8, 2013). To further humanize the CD1d/NKT lipid presentation system, we have introduced the human Vα24 TCR transgene into the KI mice. These mice have comparable numbers of iNKT cells in thymus and spleen, but significant reduced numbers in liver comparing to Vα24Tg mice. These iNKT cells predominantly express mouse Vβ8 TCR, the homolog of human Vβ11, with reduced CD4 expression. Importantly, we detected in these mice a unique population of CD8+ iNKT cells, which exist in human, but not in wild type mice. Bone marrow transfer experiments suggested that CD8+ iNKT cells are hCD1d-dependent. These CD8+ iNKT cells produce cytokines upon activation and are cytotoxic to B16 melanoma cells in vitro. The hCD1d KI-Vα24Tg mice will allow more accurate in vivo modeling of the human CD1d lipid presentation to human iNKT cells and will facilitate the preclinical assessment of iNKT cell-based therapies.

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43

Marton, N., OT Kovács, E. Baricza, D. Győri, A. Mócsai, F. Meier, C. Goodyear, I. McInnes, E. Buzás, and Gy Nagy. "A7.21 The effect of extracellular vesicles on humanin vitroosteoclastogenesis." Annals of the Rheumatic Diseases 75, Suppl 1 (February 2016): A64.2—A64. http://dx.doi.org/10.1136/annrheumdis-2016-209124.152.

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44

Juhri, Muhammad Alan, and Hidayah Hariani. "The Shifting Paradigm in Maqāsidi Discourse: A Case of Modern Islamic Bioethics." An-Nida' 47, no. 2 (December 31, 2023): 194. http://dx.doi.org/10.24014/an-nida.v47i2.25957.

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The advancement of contemporary biomedical issues has brought forth two distinct responses among Muslim scholars. The traditional view, which strictly adheres to the Quran and tradition, tends to find it difficult or even outright rejects modern biomedical practices as they may conflict with religious texts. On the other hand, the rationalist perspective tends to be more open and accommodating towards modern biomedical practices as it relies on independent reasoning detached from revelation. While acknowledging the existence of the traditionalist viewpoint in the modern era, this paper focuses on the rationalist perspective, discussing how biomedical decisions are made. By examining rationalist views on several modern biomedical issues such as organ transplantation, in vitro fertilization, and blood trading, the researcher argues that the flexibility of the maqāsid al-sharīʿah principles has become the framework in the development of this biomedical field. The researcher highlights a shift in the maqāsid paradigm from a theocentric paradigm – interpreting religion with the narrow goal of 'defending God' – to an anthropocentric paradigm – interpreting religion to defend human beings and their rights. Using discourse analysis methodology, this study argues that the paradigm shift towards an anthropocentric maqāsid has integrated Sharia with human subjectivity influenced by interests. Consequently, modern biomedical issues, which address the interests of safeguarding human life, are prioritized. Finally, although such maqāsid may potentially lead to a liquid Islamic law, the researcher concludes that an anthropocentric maqāsid paradigm will be more humane, dynamic, accommodating, and responsive to the demands of human life development. Abstrak: Kemajuan isu-isu biomedis kontemporer saat ini telah memunculkan dua aliran respon yang berbeda di mata para cendekiawan muslim. Pandangan tradisional, yang dengan ketat berpedoman pada Al-Qur’an dan tradisi, cenderung sulit atau bahkan tidak menerima sama sekali praktik-praktik biomedis modern karena bertentangan dengan nash-nash agama. Sementara pandangan rasionalis cenderung lebih terbuka dan akomodatif menerima praktik-praktik biomedis modern karena berpedoman pada penilaian akal sendiri yang terlepas dari wahyu. Terlepas dari mendiskusikan keberadaan pandangan pertama (tradisionalis) di era modern ini, makalah ini akan fokus pada pandangan kedua (rasionalis) dengan mendiskusikan bagaimana putusan-putusan biomedis dikeluarkan. Dengan mengkaji pandangan-pandangan rasionalis terhadap beberapa isu biomedis modern, seperti transplantasi organ, bayi tabung, dan jual beli darah, peneliti berargumen bahwa fleksibilitas prinsip-prinsip maqāsid al-syarīah telah menjadi framework dalam pengembangan bidang biomedis ini. Di sini, peneliti menyoroti adanya pergeseran maqāsid dari paradigma teosentris; menjalankan agama dengan tujuan ‘membela Tuhan’ dalam pengertian yang sempit, ke paradigma antroposentris; menjalankan agama untuk membela manusia dan hak-haknya. Dengan menggunakan metode analisis wacana, penelitian ini berargumen bahwa pergeseran paradigma maqāsid menuju antroposentris telah menjadikan syari’ah menyatu dengan subjektivitas manusia yang dipengaruhi oleh kepentingan-kepentingan. Karenanya, isu-isu biomedis modern, yang menjawab kepentingan menjaga jiwa manusia, adalah diutamakan. Terakhir, meskipun maqāsid seperti ini berpotensi mengarah pada liquid Islamic law, peneliti berkesimpulan bahwa paradigma maqāsid antroposentris akan lebih humanis, dinamis, akomodatif, dan responsif terhadap tuntutan perkembangan kehidupan manusia.

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45

Coradduzza, Donatella, Antonella Congiargiu, Zhichao Chen, Sara Cruciani, Angelo Zinellu, Ciriaco Carru, and Serenella Medici. "Humanin and Its Pathophysiological Roles in Aging: A Systematic Review." Biology 12, no. 4 (April 6, 2023): 558. http://dx.doi.org/10.3390/biology12040558.

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Background: Senescence is a cellular aging process in all multicellular organisms. It is characterized by a decline in cellular functions and proliferation, resulting in increased cellular damage and death. These conditions play an essential role in aging and significantly contribute to the development of age-related complications. Humanin is a mitochondrial-derived peptide (MDP), encoded by mitochondrial DNA, playing a cytoprotective role to preserve mitochondrial function and cell viability under stressful and senescence conditions. For these reasons, humanin can be exploited in strategies aiming to counteract several processes involved in aging, including cardiovascular disease, neurodegeneration, and cancer. Relevance of these conditions to aging and disease: Senescence appears to be involved in the decay in organ and tissue function, it has also been related to the development of age-related diseases, such as cardiovascular conditions, cancer, and diabetes. In particular, senescent cells produce inflammatory cytokines and other pro-inflammatory molecules that can participate to the development of such diseases. Humanin, on the other hand, seems to contrast the development of such conditions, and it is also known to play a role in these diseases by promoting the death of damaged or malfunctioning cells and contributing to the inflammation often associated with them. Both senescence and humanin-related mechanisms are complex processes that have not been fully clarified yet. Further research is needed to thoroughly understand the role of such processes in aging and disease and identify potential interventions to target them in order to prevent or treat age-related conditions. Objectives: This systematic review aims to assess the potential mechanisms underlying the link connecting senescence, humanin, aging, and disease.

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46

C.P. "Rôle de l'interleukine-4 dans perméabilité aux macromolécules de l'épithélium intestinal humain." Revue Française d'Allergologie et d'Immunologie Clinique 40, no. 6 (October 2000): 660. http://dx.doi.org/10.1016/s0335-7457(00)80146-x.

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47

Devouassoux, G. "Le basophile humain et la réponse immunitaireHuman basophils and the immune response." Revue Française d'Allergologie et d'Immunologie Clinique 44, no. 1 (January 2004): 1–8. http://dx.doi.org/10.1016/s0335-7457(03)00302-2.

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Ren, Lijie, Qiang Li, Zhao Zeng, Peipei Mou, Xiaohui Liu, Xiaorong Tang, Qiongyu Lu, Xingshun Xu, and Li Zhu. "A Humanin Analog Attenuates Platelet Responses to Vascular Injury." Blood 118, no. 21 (November 18, 2011): 1131. http://dx.doi.org/10.1182/blood.v118.21.1131.1131.

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Abstract Abstract 1131 Humanin (HN), a 24-amino acid endogenous antiapoptotic peptide, was initially shown to protect against neuronal cell death by Alzheimer's disease-related insults. It has recently been found that an exogenous analog of HN (HNG) in which the 14th amino acid serine is replaced with glycine protected against cerebral and cardiac ischemia reperfusion (I/R) injury in cortical neurons and cardiomyocytes, respectively. Platelet activation and thrombus formation has been shown to play an important role during I/R injury by exacerbating the extent of the infarct size. However, it is presently unknown whether HNG affects platelet function and the subsequent arterial thrombus formation. We thus examined whether HNG affects platelet activation and thrombus formation both in vitro and in vivo. Human platelets were isolated from healthy adults. Preincubation of washed human platelets with HNG (4μM) reduced collagen- or convulxin-induced platelet aggregation by 56.8% (P<0.05) and 71.9% (P<0.001), respectively. Similarly, HNG significantly reduced ATP release stimulated by collagen or convulxin. Convulxin-induced P-selectin expression and fibrinogen binding on single platelet was inhibited by HNG, as measured by flow cytometry. Moreover, HNG reduced platelet spreading on the fibrinogen coated surface by 62.9 % (P <0.05). Western blot revealed a reduction of platelet AKT phosphorylation by HNG upon collagen stimulation, implying the involvement of PI3K pathway. In addition, MAPK P38 phosphorylation by collagen and convulxin was also reduced by HNG. HNG effects on thrombus formation were tested in vivo in a ferric chloride-induced carotid artery injury model in mice. The intraperitoneal injection of HNG (25μg/kg) to male C57BL6/J mice significantly extended the first occlusion time (7.3±0.4 min, N=10), when compared to the saline injected littermates (5.4±0.7 min, N=12) (P <0.05). Furthermore, the number of mice that formed stable thrombus was less in the HNG–treated group (3/13) than the control group (6/13), while the non-occlusion mouse number was more in the HNG-treated group (3/13) than the control group (1/13). Together, these data show that HNG inhibits platelet activation and arterial thrombus formation. This might suggest that the protective effects of HNG against ischemia reperfusion injury could be, in part, via attenuating platelet activation. Therefore, HNG could be a potential therapeutic agent in thrombotic and cardiovascular disorders. Disclosures: No relevant conflicts of interest to declare.

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K.R.S., Dr Prasad. "Development and Validation of a New HPLC Bio Analytical Internal Standard Method for the Analysis of Two Immunology Drugs (Lamivudine and Dolutegravir) in Human Plasma." International Journal of Psychosocial Rehabilitation 24, no. 5 (April 20, 2020): 3628–37. http://dx.doi.org/10.37200/ijpr/v24i5/pr202071.

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Mahajan, Supriya D., Neil U. Parikh, Trent M. Woodruff, James N. Jarvis, Molly Lopez, Teresa Hennon, Patrick Cunningham, Richard J. Quigg, Stanley A. Schwartz, and Jessy J. Alexander. "C5a alters blood-brain barrier integrity in a humanin vitromodel of systemic lupus erythematosus." Immunology 146, no. 1 (July 15, 2015): 130–43. http://dx.doi.org/10.1111/imm.12489.

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