Готові списки джерел за темами / Immunohisto-chemistry

Добірка наукової літератури з теми "Immunohisto-chemistry"

Автор: Grafiati

Опубліковано: 10 грудня 2022

Оновлено: 28 січня 2023

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Статті в журналах з теми "Immunohisto-chemistry"

Takenoue, T., J. Kitayama, Y. Takei, N. Umetani, K. Matsuda, M. E. Nita, K. Hatano, T. Tsuruo, and H. Nagawa. "Characterization of dihydropyrimidine dehydrogenase on immunohisto-chemistry in colon carcinoma, and correlation between immunohisto-chemical score and protein level or messenger RNA expression." Annals of Oncology 11, no. 3 (March 2000): 273–80. http://dx.doi.org/10.1023/a:1008337913456.

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WATTLE, O. "Cytokeratins of the equine hoof wall, chestnut and skin: bio- and immunohisto-chemistry." Equine Veterinary Journal 30, S26 (June 10, 2010): 66–80. http://dx.doi.org/10.1111/j.2042-3306.1998.tb05124.x.

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A.MADEJ, JANUSZ. "Usefulness of immunohisto(cyto)chemistry, antigens and cell marker detection in cancer diagnosis and histogenesis." Medycyna Weterynaryjna 74, no. 09 (2018): 6129–2018. http://dx.doi.org/10.21521/mw.6129.

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It was shown that the unification of neoplastic nomenclature could be very challenging. It was also noted that neoplastic disease is a dual disease, involving both the presence of neoplastic cells in the organism and the resulting disturbances in the functioning of the immune system. The study also follows the principle that the general markers are examined before the specific ones, especially in neoplasms of unknown origin or those suspected of endocrine secretion, such as carcinoid tumours.

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Pusiol, Teresa, Maria Grazia Zorzi, Doriana Morichetti, and Francesco Piscioli. "Routine Use of Immunohisto-chemistry May Increase the Frequency of Hybrid Peripheral Nerve Sheath Tumors." American Journal of Dermatopathology 33, no. 6 (August 2011): 634–36. http://dx.doi.org/10.1097/dad.0b013e318200f722.

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Gnaegi, H. "Cryo Ultramicrotomy of Biological Samples and Polymers." Microscopy and Microanalysis 6, S2 (August 2000): 314–15. http://dx.doi.org/10.1017/s1431927600034061.

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IntroductionCryo Ultramicrotomy gains increasing importance for immunocyto- and immunohisto- chemistry, element analysis, morphological studies and other investigations.Major advances in immunocyto-chemistry and the sectioning of frozen hydrated specimens have been realized with the appropriate instrumentation and tools (1,2).In immuno-electron microscopy it has been found that a considerable reduction of structural damage in tissue and cells can be obtained with a modified sectioning and pick-up method using sucrose/methyl cellulose solution (3, 4).In recent years various scientists have tried to understand cryo sectioning and the problems related to the cry-osectioning process (5, 6, 7, 8).A promising attempt to eliminate one of the major artefacts in ultramicrotomy, that of compression", was made with the development of the oscillating diamond knife (9).In polymer research cryo ultramicrotomy is a well established technique.However, only little information is available about the the cryo sectioning process for the following reasons: Much cryo sectioning is performed in polymer companies.

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Shvorob, D. S., T. I. Shevchenko, and R. B. Kondratyk. "Diagnosis of molecular subtypes of colorectal cancer using immunohistochemistry." CLINICAL AND EXPERIMENTAL MORPHOLOGY 10, no. 3 (2021): 14–20. http://dx.doi.org/10.31088/cem2021.10.3.14-20.

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Colorectal cancer ranks third in the morbidity structure among all malignant tumors and includes sporadic and hereditary neoplasms. Cancer genome sequencing has revealed numerous mutation variants that determine the ways colorectal carcinoma progresses. The course, prognosis, and management strategy of the disease vary greatly depending on the subtype of a molecular tumor. This literature review discusses the latest data on the variants of colorectal cancer oncogenesis and presents the phenotypic model classification based on them. Immunohistochemistry (IHC) is suggested for determining the individual tumor characteristics. The article also clarifies the Bethesda panel used to detect microsatellite instability, markers for Lynch syndrome, and a list of IHC markers for determining the phenotypic model of colorectal carcinoma. Keywords: colorectal cancer, phenotypic models, consensus molecular subtypes (CMS), immunohisto-chemistry, Bethesda panel, Lynch syndrome

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Melendez, Mark M., Xiaoti Xu, Steve A. Mcclain, and Su-I. Daniel Huang. "Atypical fibroxanthoma in a young woman: An unusual case presentation." Canadian Journal of Plastic Surgery 15, no. 3 (September 2007): 169–72. http://dx.doi.org/10.1177/229255030701500302.

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Atypical fibroxanthoma (AFX) is an uncommon neoplasm, identified as a spindle cell tumour that is generally found in elderly patients on sun-exposed areas. The majority of cases of AFX are benign, and metastasis is a rare phenomenon. The first case in the literature of AFX is described in a young woman with no previous risk factors who presented with a three-month history of an enlarging nodule of the left nasal alar. Excision showed the lesion to be composed of hyperchromatic, pleomorphic, vacuolated spindle cells and multinucleated giant cells. The tumour cells stained positive for macrophage-histiocyte antigen alpha1-antitrypsin, neurokinin-1, CD68 and alpha1-antichymotrypsin. The present case report highlights the importance of correct diagnosis for AFX with adequate excision and by considering the histopathology and immunohisto-chemistry of its clinical differential diagnosis.

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Wilkins, Pamela A., Peggy S. Marsh, Helen Acland, and Fabio Del Piero. "Listeria Monocytogenes Septicemia in a Thoroughbred Foal." Journal of Veterinary Diagnostic Investigation 12, no. 2 (March 2000): 173–76. http://dx.doi.org/10.1177/104063870001200216.

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Listeria monocytogenes septicemia was diagnosed in a 6-day-old Thoroughbred foal. Primary clinical signs included fever, depression, diarrhea, and respiratory distress. Hematologic abnormalities included leukopenia, neutropenia, degenerative left shift, and hyperfibrinogenemia. Clinical chemistry and blood gas abnormalities included metabolic acidosis, hypoxemia, hypocapnia, hypoglycemia, and hyponatremia. Despite aggressive therapeutic intervention and intensive care, the foal died within 12 hours of admission. A postmortem examination was performed, and the primary gross lesion was bilaterally severe, focally extensive bronchopneumonia. Histopathology revealed severe subacute multifocal suppurative bronchopneumonia with necrotizing vasculitis and intralesional coccobacilli. Cultures of blood collected at admission and immediately prior to death were positive for L. monocytogenes, as were cultures obtained from lung and liver at necropsy. Immunohisto-chemical examination of formalin-fixed tissues revealed abundant intra- and extracellular L. monocytogenes antigen within the lung and intravascularly in multiple organs.

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Nicholson, Eric M., Robert A. Kunkle, Amir N. Hamir, Semakaleng Lebepe-Mazur, and Dennis Orcutt. "Detection of the Disease-Associated Isoform of the Prion Protein in Formalin-Fixed Tissues by Western Blot." Journal of Veterinary Diagnostic Investigation 19, no. 5 (September 2007): 548–52. http://dx.doi.org/10.1177/104063870701900515.

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Clinical signs of prion disease are not specific and include a variety of differential diagnoses. Serological tests and nucleic acid-based detection methods are not applicable to prion-disease-agent detection because of the unusual nature of the infectious agent. Prion-disease diagnosis is primarily conducted by means of immunodetection of the infectious agent, typically by at least 2 distinct procedures with immunohisto-chemistry and Western blot being the most informative. These approaches differ in the need for formalin-fixed and frozen or fresh tissue respectively. This work describes a method for the detection of the disease-associated isoform of the prion protein by Western blot using formalin-fixed tissues. The approach requires only minimal modification of existing Western-blot procedures and could readily be incorporated into existing detection schemes for confirmatory purposes when fresh or frozen tissues are unavailable.

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Більше джерел

Дисертації з теми "Immunohisto-chemistry"

Cook, Naomi Louise. "Characterisation of substance P and transient receptor potential melastatin channel messenger RNA and protein expression in acute and chronic neurological disorders." Thesis, 2010. http://hdl.handle.net/2440/64011.

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Traumatic brain injury (TBI) is the leading cause of death and disability in people under 40 years of age, with motor vehicle incidents accounting for the majority of severe TBI cases. Despite the public health burden of TBI, there are no effective treatment options available, with survivors often left with debilitating long-term deficits. Following TBI, a cascade of pathophysiological processes is initiated in the central nervous system, including oedema, inflammation, magnesium decline and oxidative stress. These factors play a role in the high morbidity and mortality following TBI, however, their underlying molecular mechanisms remain poorly understood. Parkinson’s Disease (PD) is a common neurodegenerative disease and affects approximately 1 % of the population over 65 years of age. PD is characterised by the progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta, leading to a reduction of dopamine levels in the striatum. The pathogenesis of PD is poorly understood, but is likely to involve oxidative stress and inflammatory processes. Current treatments that replace dopamine lose efficacy after several years. Treatments for TBI and PD are thus urgently required; this requires a greater understanding of the pathophysiology of these disorders at a molecular level. Recent studies from our laboratory have demonstrated a link between the neuropeptide, substance P (SP), and the development of cerebral oedema and neurologic deficits following TBI, which are attenuated with the administration of an NK-1 (neurokinin-1, SP receptor) antagonist. In addition, studies using a rat model of PD have similarly established a putative role for SP in this disease process. Transient receptor potential melastatin (TRPM) channels are a diverse family of ion channels, many of which are highly expressed in the brain. It is likely that TRPM7 and TRPM6 regulate cellular magnesium homeostasis. TRPM7 and TRPM2 are critical mediators of ischaemic neuronal death, and mutations in the TRPM7 and TRPM2 genes confer a genetic susceptibility to parkinsonism. The function of TRPM3 is not well understood, but evidence suggests it may be involved in brain function. The aims of the present thesis were to: quantify the mRNA level and protein expression of SP, TRPM2, TRPM3, TRPM6 and TRPM7 channels following TBI in human clinical cases and over a time course of experimental TBI in rats; and to characterise the mRNA level of SP, TRPM2, TRPM3 and TRPM7 channels in both clinical PD cases and two rodent models of PD (early and late disease stage), and the protein expression of TRPM channels in early experimental PD. We demonstrate an upregulation of SP expression in clinical and experimental TBI, supporting our previous studies implicating SP release with TBI pathophysiology. Changes in TRPM channel expression at both the transcript and protein level were also observed following both TBI and in PD, suggesting that TRPM channels may contribute to the oxidative stress, inflammation and neuronal death associated with these disorders. This thesis provides novel information regarding the molecular mechanisms underlying TBI and PD, which is relevant to the development of effective treatment strategies.
Thesis (Ph.D.) -- University of Adelaide, School of Medical Sciences, 2010

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Частини книг з теми "Immunohisto-chemistry"

HMJEURISSEN1, S., E. CLAASSEN2, and R. LBOYD3. "Immunohisto- chemistry in the analysis of lymphoid organs and their function." In Immunology Methods Manual, 2233–45. Elsevier, 1996. http://dx.doi.org/10.1016/b978-012442710-5/50253-7.

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Kaur, Harpreet, Svetlana Golovko, Mikhail Y. Golovko, Surjeet Singh, Diane C. Darland, and Colin K. Combs. "Effects of Probiotic Supplementation on Short Chain Fatty Acids in the AppNL–G–F Mouse Model of Alzheimer’s Disease." In Advances in Alzheimer’s Disease. IOS Press, 2022. http://dx.doi.org/10.3233/aiad220028.

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Background: The intestinal microbiota and its metabolites, particularly short-chain fatty acids (SCFAs), have been implicated in immune function, host metabolism, and even behavior. Objective: This study was performed to investigate whether probiotic administration influences levels of intestinal microbiota and their metabolites in a fashion that may attenuate brain changes in a mouse model of Alzheimer’s disease (AD). Methods: C57BL/6 wild-type (WT) mice were compared to AppNL–G–F mice. The animals were treated with either vehicle or probiotic (VSL#3) for 8 weeks. Fecal microbiome analysis along with Aβ, GFAP, Iba-1, c-Fos, and Ki-67 immunohisto-chemistry was done. SCFAs were analyzed in serum and brains using UPLC-MS/MS. Results: Probiotic (VSL#3) supplementation for 2 months resulted in altered microbiota in both WT and AppNL–G–F mice. An increase in serum SCFAs acetate, butyrate, and lactate were found in both genotypes following VSL#3 treatment. Propionate and isobutyrate were only increased in AppNL–G–F mice. Surprisingly, VSL#3 only increased lactate and acetate in brains of AppNL–G–F mice. No significant differences were observed between vehicle and VSL#3 fed AppNL–G–F hippocampal immunoreactivities of Aβ, GFAP, Iba-1, and Ki-67. However, hippocampal c-Fos staining increased in VSL#3 fed AppNL–G–F mice. Conclusion: These data demonstrate intestinal dysbiosis in the AppNL–G–F mouse model of AD. Probiotic VSL#3 feeding altered both serum and brain levels of lactate and acetate in AppNL–G–F mice correlating with increased expression of the neuronal activity marker, c-Fos.

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Тези доповідей конференцій з теми "Immunohisto-chemistry"

Kulik, M. J., D. S. Shenoda, and C. R. Forest. "A Low-Cost, Two-Axis, Precision Robot for Automated Fluorescence In-Situ Hybridization Assays." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-13272.

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Genetics research often relies on experiments that require repetitive, time-consuming handling of small volumes of liquid (1 mL) and biomass (10–20 μL) such as fluorescence in-situ hybridization (FISH), β-galactosidase staining, immunohisto chemistry, skeletal and tunel assays. Often manual, these experiments are time intensive and error-prone. We report on the design, fabrication, and testing of a low-cost, two-axis, precision robot for FISH assays on whole mice embryos. The robot can complete 20 successive embryo immersions in unique isothermal solutions in minutes for 6 samples. Repeatability of the orthogonal axes is 66 and 214 μm, near the measurement uncertainty limit and sufficient for operation. Accuracy is achieved by systematic error compensation. Low-cost and precision are obtained using design and manufacturing techniques and processes, resulting in a cost of 15% of comparable instruments (e.g., InsituStain, Intavis Bioanalytical Instruments). This design demonstrates a simple, automated platform to perform a typically manual experimental genetics technique.

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Wilkinson, J. M., N. Hack, L. I. Thorsen, and J. A. Thomas. "MONOCLONAL ANTIBODIES RECOGNISING PROTEINS OF THE OUTER AND INNER SURFACE OF THE PLATELET PLASMA MEMBRANE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644493.

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Platelet membrane preparations can be fractionated into two major subpopulations by free flow electrophoresis and these have been shown to correspond to the plasma membrane and the endoplasmic reticulum of the platelet. The plasma membrane fraction can be shown, by two-dimensional electrophoresis, to contain the major surface glycoproteins together with considerable amounts of actin and actin-associated proteins such as the 250 kDa actin-binding protein (filamin), P235 (talin), myosin, α-actinin and tropomyosin (Hack, N. … Crawford, N., Biochem. J. 222, 235 (1984). These cytoskeletal proteins are associated with the cytoplasmic face of the plasma membrane and probably interact with transmembrane glycoproteins. We have raised monoclonal antibodies to the purified plasma membrane preparation in order to investigate the nature of these glycoprotein-cytoskeletal interactions. In two fusion experiments, out of 804 tested, 104 hybrids secreted antibody to the membrane preparation and of these 24 were selected for further study. Initial assays were by ELISA using either the membrane preparation or whole fixed platelets as the target antigen. The specificity of the antibodies was investigated further by immunoblotting of SDS gels of total platelet proteins prepared under reducing and nonreducing conditions, by immunofluorescence, by immunohisto-chemistry and by crossed immunoelectrophoresis. The majority of the antibodies recognise major surface glycoproteins; of these, four bind to glycoprotein Ib under all conditions examined while another seven recognise the glycoprotein IIb/IIIa complex as detected by crossed immunoelectrophoresis. Three antibodies recognise the actin binding protein and these cross-react with the smooth muscle protein filamin in a number of different species. Further characterisation of these antibodies in both structural and functional terms will be presented.We are grateful to the Smith and Nephew Foundation for financial support for these studies

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