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Статті в журналах з теми "Imagerie multiplexé en fluorescence"
Fragola, A., L. Aigouy, S. Grésillon, and Y. De Wilde. "Imagerie de fluorescence en champ proche." Journal de Physique IV (Proceedings) 12, no. 5 (June 2002): 303–5. http://dx.doi.org/10.1051/jp4:20020172.
Повний текст джерелаMarchesini, S., G. Faigel, M. Tegze, M. Belakhovsky, and O. Ulrich. "Imagerie à l'échelle atomique par holographie de fluorescence." Le Journal de Physique IV 11, PR7 (October 2001): Pr7–165—Pr7–169. http://dx.doi.org/10.1051/jp4:2001750.
Повний текст джерелаMcLane, Michael, Glenn Milton, Linying Liu, Rachel Schaefer, Yi Zheng, Carla Coltharp, Peter Miller, and Clifford Hoyt. "46 A novel H&E-like staining method compatible with multiplexed IF on the same tissue section for integrated translational workflows." Journal for ImmunoTherapy of Cancer 8, Suppl 3 (November 2020): A49. http://dx.doi.org/10.1136/jitc-2020-sitc2020.0046.
Повний текст джерелаSupatto, Willy. "Microscopie multiphoton illuminée par nappe : imagerie de fluorescence rapide et en profondeur dans les tissus vivants." Photoniques, no. 62 (November 2012): 33–37. http://dx.doi.org/10.1051/photon/20126233.
Повний текст джерелаPatout, M., M. Salaün, P. Bohn, X. Brune, A. Romieu, F. Guisier, N. Vasseur, R. Sesboüé, P. Y. Renard, and L. Thiberville. "Imagerie moléculaire en temps réel des mutations de l’EGFR en microscopie confocale fibrée en fluorescence (MCFF)." Revue des Maladies Respiratoires 31, no. 7 (September 2014): 659. http://dx.doi.org/10.1016/j.rmr.2014.04.034.
Повний текст джерелаAmode, R., H. Colboc, K. Medjoubi, A. Somogyi, J. D. Bouaziz, V. Descamps, D. Bazin, P. Reygagne, L. Michel, and I. Lucas. "Détection folliculaire du titane au cours de l’alopécie frontale fibrosante (AFF) et du lichen plan pilaire (LPP) par imagerie de nano-fluorescence X." Annales de Dermatologie et de Vénéréologie 146, no. 12 (December 2019): A132. http://dx.doi.org/10.1016/j.annder.2019.09.158.
Повний текст джерелаLesur, O., and L. Thiberville. "Vers une évaluation in vivo, en temps réel, de la réparation pulmonaire dans le SDRA : une place pour la micro-imagerie de fluorescence par laser confocal ?" Réanimation 18, no. 2 (March 2009): 111–13. http://dx.doi.org/10.1016/j.reaurg.2009.01.016.
Повний текст джерелаBoland, F. X., L. Thiberville, J. M. Péron, F. Roussel, J. Métayer, and J. P. Marie. "O 9-4 Validation chez l’animal d’une imagerie microscopique confocale fibrée (réflectance et fluorescence) pour le diagnostic des lésions précancéreuses des voies aéro-digestives supérieures : conception du modèle animal." Revue de Stomatologie et de Chirurgie Maxillo-faciale 106, no. 4 (September 2005): 36. http://dx.doi.org/10.1016/s0035-1768(05)85943-1.
Повний текст джерела"Erratum of the article: « Imagerie de la microcirculation pulmonaire in vivo par microscopie confocale fibrée en fluorescence chez le lapin en normoxie et en hypoxie » [Rev Mal Respir 2010;27:278–82]." Revue des Maladies Respiratoires 27, no. 9 (November 2010): 1124. http://dx.doi.org/10.1016/j.rmr.2010.09.018.
Повний текст джерелаДисертації з теми "Imagerie multiplexé en fluorescence"
Chouket, Raja. "New dimensions for multiplexed fluorescence imaging." Electronic Thesis or Diss., Sorbonne université, 2019. http://www.theses.fr/2019SORUS606.
Повний текст джерелаOur research group had previously developed the OPIOM protocols for fluorescence imaging. By exploiting their cross sections of fluorescence photoswiching, OPIOM can selectively extract the response of reversibly photoswitchable fluorophores (RSFs) in the presence of spectrally interfering fluorophores. However, OPIOM allowed us to discriminate only 3 spectrally similar reversibly photoswitchable fluorescent proteins (RSFPs). The goal of this PhD was to augment this number. To reach this goal, a new automated instrumental setup called photoswichometer was first developed to express and screen the rich photochemical signature of 22 RSFPs by analyzing their fluorescence response to light jumps with intensities covering 5 orders of magnitude. This signature has been first exploited in a new fluorescence imaging protocol called HIGHLIGHT, which capitalized on OPIOM and further improved its selectivity. In HIGHLIGHT, the RSFs are submitted to harmonic light modulation and their contribution to the overall fluorescence emission signals is selectively retrieved from exploiting their singular non-linear response under optimized conditions. HIGHLIGHT has been implemented to image RSFPs in cells without interference of autofluorescence, to perform multiplexed imaging of 3 RSFPs which could not be discriminated with OPIOM, and used for its intrinsic optical sectioning. The RSF signature has been then used in a second fluorescence imaging protocol called LIGHTNING. In contrast to OPIOM and HIGHLIGHT which exploit the cross sections of fluorescence photoswitching in a steady-state regime of low light intensity, LIGHTNING exploits the transient time fluorescence response of RSFs under multiple illuminations involving various ranges of light intensities for RSF discrimination. Thus, LIGHTNING allowed us to improve the multiplexing degree of dynamic contrast in fluorescence imaging up to 20 RSFP among 22 studied RSFPs
Deiss, Frédérique. "Développement de réseaux multiplexés de biocapteurs électrochimiques." Thesis, Bordeaux 1, 2009. http://www.theses.fr/2009BOR13883/document.
Повний текст джерелаThis work presents the development of optoelectrochemical micro- and nanosensor arrays for bioanalytical applications. These platforms respond to the growing need in research and diagnostic for tools allowing multiple and simultaneous analysis in small-volume samples. These new high density biochips are made from coherent optical fiber bundles: one face is micro- or nanostructured by chemical etching and then functionnalized with biological probes. The first biochip is a fluorescent DNA nanosensor array where probes have been immobilized by electrodeposition of a polypyrrole thin film. The detection of the hybridization is remotely performed through the imaging fiber. Different probes were succesfully addressed onto the same nanostructured array thanks to electrochemical cantilevers. The second biochip allows multiplexed sandwich immunoassays using electrochimiluminescent imaging resolved at the single bead level. In particular, the development of this new readout mechanism allows extending electrochemiluminescent detection for multiplexed immunoassays. Design and implementations of both platforms take advantages of different physical and chemical techniques, especially electrochemical, to obtain biochips with a great potential through high density and high multiplexing level
Valisa, Paolo. "Imagerie spectrale en raman et fluorescence : developpement et applications (doctorat : pharmacie)." Reims, 1997. http://www.theses.fr/1997REIMP204.
Повний текст джерелаProvencher, David. "Imagerie 3D de l'anatomie interne d'une souris par dynamique de fluorescence." Mémoire, Université de Sherbrooke, 2012. http://hdl.handle.net/11143/6205.
Повний текст джерелаMaunoury, Vincent. "Imagerie de fluorescence ph dependante en oncologie : approche experimentale chez l'animal." Lille 2, 1994. http://www.theses.fr/1994LIL2P264.
Повний текст джерелаLauffer, Mathieu. "Reconnaissance d'organismes aquatiques envahissants par traitement d'image et imagerie de fluorescence." Thesis, CentraleSupélec, 2015. http://www.theses.fr/2015SUPL0024.
Повний текст джерелаPhytoplankton plays a fundamental role in the living world. It is a dioxygen generator and the most important carbon dioxide fixer on Earth. However, under certain conditions, its development may become so excessive that it could be harmful to other vegetal and animal aquatic life in the water ponds in which it grows: it is the “hyper eutrophication” phenomenon. Such a situation leads to dramatic consequences on environment due to the difficulties that arise for photosynthesis and gas exchanges of other plant species. At the extreme limit, this can cause the death of the whole aquatic ecosystem. It appears therefore essential to strengthen the vigilance on controlling the proliferation of plankton and toxins with the necessity of risk evaluation. In a first approach, the recognition and the identification of aquatic organisms, necessary for such a control, are usually performed only by specialists algologists from microscopic observations. Nevertheless, in certain circumstances, it may be useful to dispose of an automatic recognition system to improve the monitoring of high-risk water ponds and optimize human intervention of specialists algologists. The development of such an automatic system of recognition of aquatics organism is more and more considered.In order to identify aquatic organisms, an original optical microscopy set up was developed in which the incident and emitted light beams are filtered in wavelengths. Such a set up enables the acquisition of classical microscopic images and microscopic images of fluorescence emission of the vegetal material under different illumination. These different images are then analyzed and processed by two algorithms of segmentation to collect characteristics data of the vegetals morphology and pigments compositions useful thereafter for their automatic recognition. Finally, all these different characteristic parameters linked to morphology and fluorescence emission of the vegetal species are collected to build a database useful for automatic optical recognition
Piraux, Hélène. "Nanoparticules magnétiques : Vectorisation par la voie principale d'acquisition du fer : Elaboration et physico-chimie." Paris 7, 2014. http://www.theses.fr/2014PA077219.
Повний текст джерелаDue to their magnetic properties, iron oxide nanoparticles are widely used for their significant role in the diagnostic and treatment of cancer. One of the main challenges of magnetic nanoparticles in nanomedicine is their effective targeting, which consists of their fast and precis( delivery directly into destination cells. Transferrin is one of the two proteins involved in the major iron acquisition pathway. Indeed, via its interaction with Receptor 1, transferrin crosses the plasma membrane within few minutes by receptor-mediated endocytosis. Furthermore, the overexpression of transferrin-receptor 1 in cancer tells enhances transferrin internalization making it a perfect Trojan horse for nanoparticles delivery. In this work, 3 different sizes of maghemite nanoparticles (5, 10 and 15 nm) were synthetized by the polyol method, coated with 3-aminopropyltriethoxysilane and coupled to transferrin. For each size, the ratio of transferrin per nanoparticle was determined and the interaction in vitro with the receptor was investigated. Then a comparative study of internalization was conducted in cellulo between raw and grafted to transferrin nanoparticles. The efficiency of the targeting was analyzed by magnetophoresis and confocal fluorescence microscopy. All grafted nanoparticles were rapidly internalized in HeLa cells. Thus a relationship between the size of th( constructs and their efficacy in nanoparticles delivery was established. A comparison of our resuits with those of the literature shows a promising model for theragnostic devices
Kieleck, Christelle. "Imagerie de fluorescence induite par laser : application à la reconnaissance des groupes de macroalgues." Brest, 2000. http://www.theses.fr/2000BRES2049.
Повний текст джерелаLe, Cardinal de Kernier Isaure. "Cytométrie par imagerie grand champ en phase et fluorescence : applications en hématologie." Thesis, Aix-Marseille, 2019. http://theses.univ-amu.fr.lama.univ-amu.fr/191018_LECARDINALDEKERNIER_341bnro964jhs311fcdqc638rrosk_TH.pdf.
Повний текст джерелаBlood cell population analyses allow detecting a wide scope of clinical disorders, ranging from anemias to malaria. A very large number of cells ought to be considered so as to ensure the statistical significance of the result, and in turn, yield a reliable diagnosis. Currently, hematology analyses are based on flow cytometry techniques. High throughput is obtained at the expense of the information content of each acquisition. To reduce the time-to-result, and to minimize the complexity and cost of the systems dedicated to analyzing cell populations, the current need is to reduce the number of acquisitions and optimize the information content. This thesis focuses on single-shot image cytometry as an alternative to flow-based cytometry. It aims at obtaining a set-up based on optical contrasts for the study of large cell populations while preserving the ability to resolve individual cells. We investigate a multi-scale and multi-modal approach to detect, characterize, and classify blood cells. To evaluate the feasibility and clinical relevance of the method, we developed two proof-of-concept set-ups, respectively called the mesoscope and the miniscope. The mesoscope, based on optical developments, combines phase contrast with fluorescence. The complementarity of morphological features and the expression of specific fluorophores enables us to accurately classify blood cells, and for example assess Plasmodium falciparum parasitemia in whole blood samples. The results are benchmarked to reference techniques. However, to address the need for point of care analyses, the system should be miniaturized. Hence, we designed the miniscope, a chip-based bimodal imager
Pliquett, Jacques. "Development of fluorescent platforms for the design of multifunctional compounds for in vitro and in vivo applications in molecular imaging." Thesis, Bourgogne Franche-Comté, 2018. http://www.theses.fr/2018UBFCK067.
Повний текст джерелаThe objective of this thesis was the development and evaluation of new molecular platformsfor optical fluorescence imaging applications. This work sought to develop new tools that caneasily be modified and adapted to the specific needs of the intended use. This is required asthe fluorophore will influence the final properties and should thus be incorporated beforestructural optimization of the selected agent rather than at the very end. Two main axes wereexplored; the use of BODIPYs for the development of trackable therapeutic agents that areprimarily intended for in vitro applications and the use of azaBODIPYs for the design of an invivo compatible fluorescent platform.In the first part two fluorophores on the basis of a 3,5-dichloro-BODIPY were identified aspromising platforms. These platform molecules were selectively functionalized using a gold(I)-phosphine moiety, a thiosugar and a phosphonium to explore their selective functionalizationand investigate the influence of each substitutents position on the final properties. Weshowed that a site-specific, selective functionalization with these fragile substituents ispossible and developed 12 gold(I)-bearing therapeutic agents. We evaluated thephotophysical properties of all obtained compounds which was followed by a characterizationof their biological properties (antiproliferative properties on 3 cancer cell lines, lipophilicbalance and cellular gold accumulation as well as fluorescence imaging on 3 cell lines for upto 24h). We succeeded in developing a panel of closely related trackable compounds thatdisplay mixed activity in cells and distinct cellular localization. This investigation permitted theselection of three to four hits that will be studied further.In the second part we developed an in vivo-compatible multifunctional platform following twostrategies: the first was the use of 1,7-di(phenol)-3,5-di(phenyl)-azaBODIPY and thefunctionalization of the hydroxy groups for the development of a bioconjugable NIR-I probe.Unfortunately the developed probe displayed very unfavourable optical properties; wetherefore developed a new strategy that is entirely based on the functionalization of the boronatom. Using this approach we successfully synthesized 2 watersoluble, strongly fluorescent(NIR-I) molecular platforms that were conjugated to an innovative antibody to image the PD-L1 ligand. The developed probes displayed excellent optical properties, are stable for at least48h in mice plasma and were validated in a preclinical study on mice. The developed probesdisplayed strong fluorescence in vivo and showed no acute toxicity.The developed methodology shows great potential for further investigations and futurestudies; it can be transposed onto other closely related fluorophores and permits versatilefunctionalization with a large variety of compounds of interest. Its use is thus not limited tobiological, biochemical and medical applications
Книги з теми "Imagerie multiplexé en fluorescence"
Fluorescence Imaging and Biological Quantification. Taylor & Francis Group, 2019.
Знайти повний текст джерелаFluorescence Imaging and Biological Quantification. Taylor & Francis Group, 2017.
Знайти повний текст джерелаSuri, Jasjit S., J. Miquel Sanches, and Raquel Seruca. Fluorescence Imaging and Biological Quantification. Taylor & Francis Group, 2017.
Знайти повний текст джерелаSuri, Jasjit S., J. Miquel Sanches, and Raquel Seruca. Fluorescence Imaging and Biological Quantification. Taylor & Francis Group, 2017.
Знайти повний текст джерелаSuri, Jasjit S., J. Miquel Sanches, and Raquel Seruca. Fluorescence Imaging and Biological Quantification. Taylor & Francis Group, 2017.
Знайти повний текст джерелаMarcu, Laura, Paul M. W. French, and Daniel S. Elson. Fluorescence Lifetime Spectroscopy and Imaging. Taylor & Francis Group, 2020.
Знайти повний текст джерелаFluorescence Lifetime Spectroscopy and Imaging. CRC Press, 2012.
Знайти повний текст джерелаMarcu, Laura, Paul M. W. French, and Daniel S. Elson. Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics. Taylor & Francis Group, 2014.
Знайти повний текст джерелаMarcu, Laura, Paul M. W. French, and Daniel S. Elson. Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics. Taylor & Francis Group, 2014.
Знайти повний текст джерелаThe Fluorescent Protein Revolution. CRC Press, 2012.
Знайти повний текст джерелаЧастини книг з теми "Imagerie multiplexé en fluorescence"
RIGNEAULT, Hervé, and Julien DUBOISSET. "Imagerie Raman cohérente." In Spectroscopies vibrationnelles, 273–88. Editions des archives contemporaines, 2020. http://dx.doi.org/10.17184/eac.4204.
Повний текст джерелаТези доповідей конференцій з теми "Imagerie multiplexé en fluorescence"
Garcia, N. Missael, Tyler Davis, Kevin Kauffman, Radoslav Marinov, and Viktor Gruev. "A Six-channel Multispectral Imager for Simultaneous In Vivo Imaging of Multiple Near-Infrared Fluorescent Markers." In Clinical and Translational Biophotonics. Washington, D.C.: OSA, 2018. http://dx.doi.org/10.1364/translational.2018.jtu3a.3.
Повний текст джерела