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Добірка наукової літератури з теми "Hydrogen deuterium exchange (HDx)"

Автор: Grafiati
Опубліковано: 14 березня 2023

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Статті в журналах з теми "Hydrogen deuterium exchange (HDx)"

1

Masson, Glenn R., Sarah L. Maslen, and Roger L. Williams. "Analysis of phosphoinositide 3-kinase inhibitors by bottom-up electron-transfer dissociation hydrogen/deuterium exchange mass spectrometry." Biochemical Journal 474, no. 11 (May 16, 2017): 1867–77. http://dx.doi.org/10.1042/bcj20170127.

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Until recently, one of the major limitations of hydrogen/deuterium exchange mass spectrometry (HDX-MS) was the peptide-level resolution afforded by proteolytic digestion. This limitation can be selectively overcome through the use of electron-transfer dissociation to fragment peptides in a manner that allows the retention of the deuterium signal to produce hydrogen/deuterium exchange tandem mass spectrometry (HDX-MS/MS). Here, we describe the application of HDX-MS/MS to structurally screen inhibitors of the oncogene phosphoinositide 3-kinase catalytic p110α subunit. HDX-MS/MS analysis is able to discern a conserved mechanism of inhibition common to a range of inhibitors. Owing to the relatively minor amounts of protein required, this technique may be utilised in pharmaceutical development for screening potential therapeutics.
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Bouyssié, David, Jean Lesne, Marie Locard-Paulet, Renaud Albigot, Odile Burlet-Schiltz, and Julien Marcoux. "HDX-Viewer: interactive 3D visualization of hydrogen–deuterium exchange data." Bioinformatics 35, no. 24 (July 9, 2019): 5331–33. http://dx.doi.org/10.1093/bioinformatics/btz550.

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Abstract Summary With the advent of fully automated sample preparation robots for Hydrogen–Deuterium eXchange coupled to Mass Spectrometry (HDX-MS), this method has become paramount for ligand binding or epitope mapping screening, both in academic research and biopharmaceutical industries. However, bridging the gap between commercial HDX-MS software (for raw data interpretation) and molecular viewers (to map experiment results onto a 3D structure for biological interpretation) remains laborious and requires simple but sometimes limiting coding skills. We solved this bottleneck by developing HDX-Viewer, an open-source web-based application that facilitates and quickens HDX-MS data analysis. This user-friendly application automatically incorporates HDX-MS data from a custom template or commercial HDX-MS software in PDB files, and uploads them to an online 3D molecular viewer, thereby facilitating their visualization and biological interpretation. Availability and implementation The HDX-Viewer web application is released under the CeCILL (http://www.cecill.info) and GNU LGPL licenses and can be found at https://masstools.ipbs.fr/hdx-viewer. The source code is available at https://github.com/david-bouyssie/hdx-viewer.
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Gogonea, Valentin, Judith Peters, Gary S. Gerstenecker, Celalettin Topbas, Liming Hou, Jérôme Combet, Joseph A. DiDonato, Jonathan D. Smith, Kerry-Anne Rye, and Stanley L. Hazen. "Protein Backbone and Average Particle Dynamics in Reconstituted Discoidal and Spherical HDL Probed by Hydrogen Deuterium Exchange and Elastic Incoherent Neutron Scattering." Biomolecules 10, no. 1 (January 10, 2020): 121. http://dx.doi.org/10.3390/biom10010121.

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Lipoproteins are supramolecular assemblies of proteins and lipids with dynamic characteristics critically linked to their biological functions as plasma lipid transporters and lipid exchangers. Among them, spherical high-density lipoproteins are the most abundant forms of high-density lipoprotein (HDL) in human plasma, active participants in reverse cholesterol transport, and associated with reduced development of atherosclerosis. Here, we employed elastic incoherent neutron scattering (EINS) and hydrogen-deuterium exchange mass spectrometry (HDX-MS) to determine the average particle dynamics and protein backbone local mobility of physiologically competent discoidal and spherical HDL particles reconstituted with human apolipoprotein A-I (apoA-I). Our EINS measurements indicated that discoidal HDL was more dynamic than spherical HDL at ambient temperatures, in agreement with their lipid-protein composition. Combining small-angle neutron scattering (SANS) with contrast variation and MS cross-linking, we showed earlier that the most likely organization of the three apolipoprotein A-I (apoA-I) chains in spherical HDL is a combination of a hairpin monomer and a helical antiparallel dimer. Here, we corroborated those findings with kinetic studies, employing hydrogen-deuterium exchange mass spectrometry (HDX-MS). Many overlapping apoA-I digested peptides exhibited bimodal HDX kinetics behavior, suggesting that apoA-I regions with the same amino acid composition located on different apoA-I chains had different conformations and/or interaction environments.
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Setner, B., M. Wierzbicka, L. Jerzykiewicz, M. Lisowski та Z. Szewczuk. "The unexpected racemization and hydrogen–deuterium exchange of the hydrogen at the α-carbon of proline analogs containing the 5-azoniaspiro[4.4]nonyl-group". Organic & Biomolecular Chemistry 16, № 5 (2018): 825–31. http://dx.doi.org/10.1039/c7ob02926h.

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Kazazić, Saša, Zrinka Karačić, Igor Sabljić, Dejan Agić, Marko Tomin, Marija Abramić, Michal Dadlez, Antonija Tomić, and Sanja Tomić. "Conservation of the conformational dynamics and ligand binding within M49 enzyme family." RSC Advances 8, no. 24 (2018): 13310–22. http://dx.doi.org/10.1039/c7ra13059g.

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The hydrogen deuterium exchange (HDX) mass spectrometry combined with molecular dynamics (MD) simulations was employed to investigate conformational dynamics and ligand binding within the M49 family (dipeptidyl peptidase III family).
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Song, Xiaowei, Jia Li, Mohammad Mofidfar, and Richard N. Zare. "Distinguishing between Isobaric Ions Using Microdroplet Hydrogen–Deuterium Exchange Mass Spectrometry." Metabolites 11, no. 11 (October 23, 2021): 728. http://dx.doi.org/10.3390/metabo11110728.

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Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome by using hydrogen–deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different types of functional groups, each one produces a unique MS spectral pattern after droplet spray HDX without the need for MS/MS experiments or introduction of ion mobility measurements. As an example of the power of this approach, isobaric ions in urinary metabolic profiles are identified and used to distinguish between healthy individuals and those having bladder cancer.
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Brown, Kerene A., and Derek J. Wilson. "Bottom-up hydrogen deuterium exchange mass spectrometry: data analysis and interpretation." Analyst 142, no. 16 (2017): 2874–86. http://dx.doi.org/10.1039/c7an00662d.

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Zhdanova, Polina V., Alexander A. Chernonosov, Daria V. Prokhorova, Grigory A. Stepanov, Lyubov Yu Kanazhevskaya, and Vladimir V. Koval. "Probing the Dynamics of Streptococcus pyogenes Cas9 Endonuclease Bound to the sgRNA Complex Using Hydrogen-Deuterium Exchange Mass Spectrometry." International Journal of Molecular Sciences 23, no. 3 (January 20, 2022): 1129. http://dx.doi.org/10.3390/ijms23031129.

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The Cas9 endonuclease is an essential component of the CRISPR–Cas-based genome editing tools. The attainment of high specificity and efficiency of Cas9 during targetted DNA cleavage is the main problem that limits the clinical application of the CRISPR–Cas9 system. A deep understanding of the Cas9 mechanism and its structural-functional relationships is required to develop strategies for precise gene editing. Here, we present the first attempt to describe the solution structure of Cas9 from S. pyogenes using hydrogen-deuterium exchange mass spectrometry (HDX-MS) coupled to molecular dynamics simulations. HDX data revealed multiple protein regions with deuterium uptake levels varying from low to high. By analysing the difference in relative deuterium uptake by apoCas9 and its complex with sgRNA, we identified peptides involved in the complex formation and possible changes in the protein conformation. The REC3 domain was shown to undergo the most prominent conformational change upon enzyme-RNA interactions. Detection of the HDX in two forms of the enzyme provided detailed information about changes in the Cas9 structure induced by sgRNA binding and quantified the extent of the changes. The study demonstrates the practical utility of HDX-MS for the elucidation of mechanistic aspects of Cas9 functioning.
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Dapic, Irena, Ivone Jakasa, Renata Kobetic, and Lidija Brkljacic. "Characterization of Ceramides with Phytosphingosine Backbone by Hydrogen-deuterium Exchange Mass Spectrometry." Croatica chemica acta 92, no. 3 (2019): 411–17. http://dx.doi.org/10.5562/cca3506.

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Ceramides are a lipid subclass of the sphingolipids that show large structural diversity. Structural characterization of the ceramides (CERs) can lead to better understanding of their role and function in the biological system. Here we investigated representatives of NP (CER III, CER IIIB) and AP ceramide classes (CER VI) that contain phytosphingosine (P) backbone. Ceramides were characterized in positive ionization mode by hydrogen-deuterium exchange mass spectrometry (HDX-MS). Fragmentation in positive ionization mode of the CER III and CER VI resulted in abundant ions assigned to phytosphingosine moiety at m/z 282, 300 and 318. HDX-MS of fragments showed increase in m/z of corresponding ions confirming the exchange of deuterium. In negative ionisation spectra multiple fragment ions were assigned to fatty acyl (RCOO–) moiety. Presence of RCOO– allowed unambiguous identification of CER III and CER IIIB which were distinguished by the presence of double bond on fatty acyl chain.
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Benhaim, Mark, Kelly K. Lee, and Miklos Guttman. "Tracking Higher Order Protein Structure by Hydrogen-Deuterium Exchange Mass Spectrometry." Protein & Peptide Letters 26, no. 1 (February 13, 2019): 16–26. http://dx.doi.org/10.2174/0929866526666181212165037.

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Background: Structural biology has provided a fundamental understanding of protein structure and mechanistic insight into their function. However, high-resolution structures alone are insufficient for a complete understanding of protein behavior. Higher energy conformations, conformational changes, and subtle structural fluctuations that underlie the proper function of proteins are often difficult to probe using traditional structural approaches. Hydrogen/Deuterium Exchange with Mass Spectrometry (HDX-MS) provides a way to probe the accessibility of backbone amide protons under native conditions, which reports on local structural dynamics of solution protein structure that can be used to track complex structural rearrangements that occur in the course of a protein’s function. Conclusion: In the last 20 years the advances in labeling techniques, sample preparation, instrumentation, and data analysis have enabled HDX to gain insights into very complex biological systems. Analysis of challenging targets such as membrane protein complexes is now feasible and the field is paving the way to the analysis of more and more complex systems.
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Дисертації з теми "Hydrogen deuterium exchange (HDx)"

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Bourguet, Maxime. "Développements méthodologiques en spectrométrie de masse structurale pour la caractérisation de complexes biologiques multiprotéiques." Thesis, Strasbourg, 2019. http://www.theses.fr/2019STRAF013.

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Ce travail de thèse porte sur le développement de méthodes de spectrométrie de masse (MS) structurale pour la caractérisation de systèmes protéiques complexes, souvent réfractaires aux approches biophysiques classiques. Dans ce contexte, les développements entrepris furent notamment focalisés sur la caractérisation de complexes impliqués dans la biogénèse des ribosomes et dans la régulation transcriptionnelle, fonctions cellulaires essentielles pouvant être liées à de nombreuses pathologies humaines dont certains cancers. Ainsi, les approches par MS native, pontage chimique et d’HDX-MS ont permis de renseigner sur la connectivité, les proximités spatiales ou encore la dynamique conformationnelle retrouvées au sein des complexes étudiés. Parmi ces techniques, l’HDX-MS permet une approche comparative basée sur les mesures d’incorporations en deutérium renseignant sur la dynamique conformationnelle d’une protéine sous différents états. Aussi, la combinaison d’approches de MS structurale a permis d’approfondir la caractérisation des systèmes complexes étudiés, démontrant ainsi l’intérêt d’une approche intégrative dans ce contexte
This PhD thesis focuses on developing methods in structural mass spectrometry (MS) to characterize complex protein systems, given their size and their heterogeneity, frequently inaccessible by classical biophysic approaches. In this context, methodological developments have particularly focused on the characterization of protein complexes involved in ribosomes biogenesis and transcriptional regulation. These fundamental cellular processes are related to numerous diseases such as cancers and genetic diseases. Thus native MS, crosslink, and hydrogen/deuterium exchange coupled to MS (HDX-MS) allowed gaining insights about the stoechiometry, spatial proximities and conformational dynamics of studied systems. Among these approaches, HDX-MS enables a comparative approach based on deuterium incorporation measurements giving information about the conformational dynamics of labeled proteins in various experimental conditions. Finally, the combination of structural approaches enables to deeply characterize complex protein systems, highlighting the advantages of an integrative approach in this context
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Durazo, Armando. "Hydrogen/deuterium exchange studies on copper-zinc superoxide dismutase." Diss., Restricted to subscribing institutions, 2007. http://proquest.umi.com/pqdweb?did=1495960591&sid=1&Fmt=2&clientId=1564&RQT=309&VName=PQD.

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Mullangi, Vennela Dr. "Development and Application of Histidine Hydrogen Deuterium Exchange Mass Spectrometry." Cleveland State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=csu1388959354.

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Stofella, Michele. "Hydrogen deuterium exchange: methods to probe protein dynamics at single residue resolution." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2020. http://amslaurea.unibo.it/21242/.

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The purpose of this work is to provide computational methods to fingerprint protein dynamics probed by hydrogen deuterium exchange mass spectroscopy (HDX-MS). Hydrogen deuterium exchange consists in the spontaneous exchange of amide hydrogens of amino acids with deuterium contained in solution. The consequent increase in mass of the protein can be monitored by mass spectroscopy. Moreover, the exchange rate (or protection factor) provides a parameter probing protein dynamics at single residue resolution. The ExPfact algorithm is a computational method implemented to extract fine-grained information out of coarse-grained HDX-MS experimental data. The method is validated through a comparison with protection factors estimated from HDX-NMR measurements probing the mouse prion protein. Also, a second application studying glycogen phosphorylase shows how structural changes between different states of the same protein can be detected at amino acidic resolution. Furthermore, fine-grained information extracted by ExPfact is coupled with a back-exchange correction to reproduce experimental spectra, suggesting that the information encoded in the centroids of the spectra is sufficient to characterize experimental data. Last but not least, an existing structural model connecting the structure of a protein to its protection factors is discusses and improved via the introduction of a dependence on the electrostatic potential of the protein.
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Tsutsui, Yuko. "EXPLORING FUNCTIONAL AND FOLDING ENERGY LANDSCAPES BY HYDROGEN-DEUTERIUM EXCHANGE MASS SPECTROMETRY." Case Western Reserve University School of Graduate Studies / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1196199391.

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Lu, Xiaojun. "STRUCTURE OF PRION PROTEIN AMYLOID FIBRILS AS DETERMINED BY HYDROGEN/DEUTERIUM EXCHANGE." Case Western Reserve University School of Graduate Studies / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1205510131.

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Hsu, Simon. "Hydrogen/deuterium-exchange (DXMS) analysis of the carbohydrate phosphatase, starch-excess 4." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2008. http://wwwlib.umi.com/cr/ucsd/fullcit?p1459886.

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Thesis (M.S.)--University of California, San Diego, 2008.
Title from first page of PDF file (viewed January 5, 2009). Available via ProQuest Digital Dissertations. Includes bibliographical references (p. 80-83).
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Mandell, Jeffrey G. "Protein-protein interactions studied by hydrogen-deuterium exchange and computer-aided docking /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2000. http://wwwlib.umi.com/cr/ucsd/fullcit?p9970664.

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NDONI, ENEA. "Characterization of the immune response and cross protection activity elicited by the Neisserial Heparin Binding Antigen (NHBA), a component of the 4CMenB vaccine." Doctoral thesis, Università di Siena, 2017. http://hdl.handle.net/11365/1011542.

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Invasive disease caused by capsular group B Neisseria meningitidis (MenB) is life threating disease causing hundred thousands of deaths every year, still remaining an unmet medical need in many countries. Although disease can be observed at all age groups, infants and adolescents are the most at risk populations showing the highest incidence in case numbers. Since the MenB capsule was not-immunogenic the development of a MenB vaccine which makes the use of other antigens becomes necessary. 4CMenB is a multicomponent vaccine against serogroup B N. meningitidis composed by three major protein antigens, factor H-binding protein (fHbp), Neisserial Heparin-Binding Antigen (NHBA) and Neisserial adhesin A (NadA), combined with outer membrane vesicles (OMVs) from the New-Zealand epidemic strain (NZ98/254). Neisserial Heparin Binding Antigen (NHBA) is a surface-exposed lipoprotein expressed by all N. meningitidis strains analyzed so far and is composed of two major domains, a highly variable amino-terminal (N-term) domain which anchors the protein on the bacterial outer membrane through the lipobox motif, and a highly conserved carboxyl-terminal (C-term) domain. These domains are separated by a short and quite conserved Arginine-rich (Arg-rich) motif which has been reported to be involved in different mechanisms that mediate meningococci adhesion, infection and survival within the host’s blood stream. NHBA is susceptible to cleavage by NalP, a bacterial protease which has its cleavage site upstream of the arginine region. Moreover human proteases such as human lactoferrin (hLf) and kallikrein are able to process NHBA downstream the the Arg-rich region. Both bacterial and human proteases-mediated cleavage releases the C-term of NHBA in the supernatant, while the N-term of the protein remains anchored on the bacterial surface. NalP cleavage did not impact SBA titers elicited by anti-NHBA antibodies but little is known about the impact that host’s proteases have on bactericidal titers. Based on sequence analysis it has been reported that NHBA has two major alleles, the so called “short” and “long” variants, which differentiate by the presence or absence of a 190 bp long fragment. Despite its sequence variability, NHBA is able to induce a robust and broad immune response against meningococcal strains expressing vaccine homologous and heterologous variants. Although anti-NHBA antibodies are able to induce bacterial killing when tested in serum bactericidal activity assay (SBA), the regions involved in eliciting cross protective immune response remain still unknown. Aims of this study were to use monoclonal antibodies (mAbs) raised against the NHBA vaccine variant peptide 2 (NHBAp2) to (i) map the NHBA regions involved in eliciting the functional response, (ii) test their ability to induce cross protection against strains expressing epidemiologically relevant homologous and heterologous NHBA variants, and (iii) investigate the molecular mechanism of NHBA-mediated bactericidal activity. To this end we used a panel of anti-NHBA mAbs selected to recognize different regions of the protein. Our results showed that only anti-N-term mAbs were able to induce killing of bacterial strains expressing the homologous NHBAp2 and closely related heterologous NHBA variants. Synergy between monoclonal antibodies targeting the N-term and the C-term of NHBA resulted in a significant increase of bactericidal titers but cross protection remained restricted to closely phylogenetic NHBA variants. Anti C-term mAbs were not able to induce SBA activity when tested individually, but surprisingly they became bactericidal when tested in combination. Moreover they were able to induce full cross protection against a panel of strains expressing phylogenetically distant heterologous NHBA variants. Our results suggest that the partial release of the NHBA C-terminal portion upon NalP and serum proteases could explain why anti-C-term mAbs are not able to induce complement mediated bactericidal killing when tested individually. However, the simultaneous binding of C-term mapping mAbs on the same NHBA molecule can induce the formation of a very stable ternary complex that probably allows a more efficient C1q engagement and C3 deposition, thus leading to the observed co-operative bactericidal activity. These results suggest that synergy between anti-NHBA antibodies is at the basis of the mechanism of NHBA-induced bactericidal activity, which could explain the robust and cross-protective immune response elicited by anti-NHBA polyclonal antibodies following immunization. Collectively, the body of experimental data suggests that both domains of NHBA are required to elicit complement mediated bactericidal activity against strains expressing the vaccine homologous and heterologous NHBA variants.
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Lau, Simon Sheen Man. "The application of capillary electrophoresis-hydrogen deuterium exchange-mass spectrometry in peptide analysis." Thesis, University of Strathclyde, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.431775.

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Книги з теми "Hydrogen deuterium exchange (HDx)"

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Milne, John Joseph. A study of tryptophan synthase catalysed hydrogen-deuterium exchange reactions. Dublin: University College Dublin, 1996.

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2

United States. National Aeronautics and Space Administration., ed. Composition and evolution of the atmosphere of Venus: Contract/grant number, NAGW 4388; period covered, 1/1/96-12/31/96. [Washington, DC: National Aeronautics and Space Administration, 1996.

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United States. National Aeronautics and Space Administration., ed. Composition and evolution of the atmosphere of Venus: Contract/grant number, NAGW 4388; period covered, 1/1/96-12/31/96. [Washington, DC: National Aeronautics and Space Administration, 1996.

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4

Weis, David D. Hydrogen-Deuterium Exchange Mass Spectrometry: Fundamentals, Techniques, and Applications. Wiley & Sons, Limited, John, 2016.

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Частини книг з теми "Hydrogen deuterium exchange (HDx)"

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Stariha, Jordan T. B., Reece M. Hoffmann, David J. Hamelin, and John E. Burke. "Probing Protein–Membrane Interactions and Dynamics Using Hydrogen–Deuterium Exchange Mass Spectrometry (HDX-MS)." In Protein-Ligand Interactions, 465–85. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1197-5_22.

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2

Kaltashov, Igor A., Cedric E. Bobst, and Rinat R. Abzalimov. "Hydrogen/Deuterium Exchange Mass Spectrometry (HDX MS) in the Studies of Architecture, Dynamics, and Interactions of Biopharmaceutical Products." In Mass Spectrometry Handbook, 227–41. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2012. http://dx.doi.org/10.1002/9781118180730.ch10.

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Yan, Xuguang, and Claudia S. Maier. "Hydrogen/Deuterium Exchange Mass Spectrometry." In Mass Spectrometry of Proteins and Peptides, 255–71. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-493-3_15.

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Kochert, Brent A., Roxana E. Iacob, Thomas E. Wales, Alexandros Makriyannis, and John R. Engen. "Hydrogen-Deuterium Exchange Mass Spectrometry to Study Protein Complexes." In Protein Complex Assembly, 153–71. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7759-8_10.

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Beckett, Dorothy. "Hydrogen–Deuterium Exchange Study of an Allosteric Energy Cycle." In Methods in Molecular Biology, 261–78. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-61779-334-9_14.

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Weis, David D., Suma Kaveti, Yan Wu, and John R. Engen. "Probing Protein Interactions Using Hydrogen-Deuterium Exchange Mass Spectrometry." In Mass Spectrometry of Protein Interactions, 45–61. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470146330.ch3.

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Singh, Harsimran, and Laura S. Busenlehner. "Probing Backbone Dynamics with Hydrogen/Deuterium Exchange Mass Spectrometry." In Protein Dynamics, 81–99. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-658-0_5.

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Filandrova, Ruzena, Daniel Kavan, Alan Kadek, Petr Novak, and Petr Man. "Studying Protein–DNA Interactions by Hydrogen/Deuterium Exchange Mass Spectrometry." In Methods in Molecular Biology, 193–219. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-1126-5_11.

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Rubens, P., J. Frank, and K. Heremans. "Hydrogen-Deuterium Exchange of Lipoxygenase at High Pressure and Temperature." In Advances in High Pressure Bioscience and Biotechnology, 235–38. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-60196-5_52.

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Baello, Bernoli I., Petr Pancoska, and Timothy A. Keiderling. "Protein hydrogen-deuterium exchange FT-IR spectroscopy and secondary structure correlation." In Spectroscopy of Biological Molecules: New Directions, 61–62. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4479-7_28.

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Тези доповідей конференцій з теми "Hydrogen deuterium exchange (HDx)"

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Zainuddin, W. N. Safawati, Sheena X. Xie, Nor Idah Kechut, Budi P. Kantaatmadja, Philip M. Singer, and George J. Hirasaki. "Hydrogen-Deuterium Exchange Between Rock Minerals and DO." In SPE Middle East Oil and Gas Show and Conference. Society of Petroleum Engineers, 2019. http://dx.doi.org/10.2118/194978-ms.

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Holt, R. J., R. Gilman, E. R. Kinney, R. S. Kowalczyk, J. Napolitano, L. Young, S. I. Mishnev, et al. "Tests of a polarized source of hydrogen and deuterium based on a spin-exchange optical pumping and a storage cell for polarized deuterium." In International symposium on high−energy spin physics. AIP, 1989. http://dx.doi.org/10.1063/1.38333.

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Lutz, E. T., J. S. Veldhuizen, Hendrik J. Luinge, John H. van der Maas, J. Baran, and H. Ratajczak. "Hydrogen bonding in crystalline beta-D-fructopyranose: a deuterium exchange and variable low-temperature FTIR study." In Luebeck - DL tentative, edited by Herbert M. Heise, Ernst H. Korte, and Heinz W. Siesler. SPIE, 1992. http://dx.doi.org/10.1117/12.56333.

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Rossegger, Bernhard, Michael Engelmayer, and Andreas Wimmer. "Challenges in Measuring Lube Oil Consumption of Internal Combustion Engines Using Deuterium As a Tracer." In ASME 2019 Internal Combustion Engine Division Fall Technical Conference. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/icef2019-7111.

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Анотація:
Abstract Lube oil emission is thought to have a negative influence on hydrocarbon and particle emissions, autoignition and the life-cycle cost of internal combustion engines. Thus, one of the major goals of combustion engine research and development is to optimize lube oil consumption, for example by optimizing the tribological behavior of the piston group (interaction between piston rings and cylinder liner). This requires the application of a fast and accurate lube oil consumption measurement method. Methods such as gravimetric and volumetric measurement are outdated for R&D applications because of measurement time, absolute accuracy as well as repeatability, however some OEMs are still applying this method. At present, the use of tracer methods for measuring lube oil consumption is considered the most promising in terms of decreasing measurement time and increasing accuracy. For example, sulfur as a tracer is one of the most established methods for measuring lube oil consumption, but previous publications have revealed downsides and future challenges of its use. This publication, however, highlights the challenges of using the stable hydrogen isotope deuterium as a tracer which are still to overcome, in order to become a viable and reliable method for measuring lube oil consumption on internal combustion engines. In the introduction, a novel concept of measuring lube oil consumption with deuterated engine oil and the test bench setup are explained. Following laboratory experiments, test bench runs on a heavy-duty diesel engine and long-term studies on a field engine, three major challenges facing the new approach are identified and potential solutions are proposed. First, the long-term stability of the tracer in the lube oil and potential changes in the physical and chemical properties of the oil due to deuteration are discussed in light of the results of tests on a field engine that uses deuterated engine oil. Second, the hydrogen-deuterium exchange process to mark the oil with the tracer is examined and potential approaches for reducing cost and duration are highlighted. The universal applicability of the deuteration process to several base oil groups is also explained. Finally, the detection of deuterium in the gas of the engine exhaust and potential cross-sensitivities to trace gases as well as other crucial limitations of the detector in analyzing engine exhaust are addressed. The summary presents the requirements for converting the experiments with a deuterium tracer into a reliable method for lube oil consumption measurement providing crucial properties such as high accuracy, short measurement time, effort and ease of use.
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Звіти організацій з теми "Hydrogen deuterium exchange (HDx)"

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Poston, David I. Control of a Uranium-Hydride Reactor with Deuterium-Hydrogen Exchange. Office of Scientific and Technical Information (OSTI), December 2013. http://dx.doi.org/10.2172/1113781.

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