Дисертації з теми "High-throughput mass spectrometry"
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Pierce, Carrie. "High throughput mass spectrometry for microbial identification." Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/43741.
Повний текст джерелаMaple, Hannah Jane. "Towards high-throughput fragment screening by mass spectrometry." Thesis, University of Bristol, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.559091.
Повний текст джерелаBoström, Tove. "High-throughput protein analysis using mass spectrometry-based methods." Doctoral thesis, KTH, Proteinteknologi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-154513.
Повний текст джерелаQC 20141022
MENG, ZHAOJING. "TOWARDS HIGH-THROUGHPUT ANALYSIS OF RNA USING MASS SPECTROMETRY." University of Cincinnati / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1098054876.
Повний текст джерелаAbdelrazig, Salah M. A. "Mass spectrometry for high-throughput metabolomics analysis of urine." Thesis, University of Nottingham, 2015. http://eprints.nottingham.ac.uk/30600/.
Повний текст джерелаLi, You. "High throughput mass spectrometry based peptide identification search engine by GPUs." HKBU Institutional Repository, 2015. https://repository.hkbu.edu.hk/etd_oa/261.
Повний текст джерелаShi, Wunan. "High-Throughput De Novo Sequencing of Transfer RNAs Using Liquid Chromatography-Tandem Mass Spectrometry." University of Cincinnati / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1378197247.
Повний текст джерелаDelabrière, Alexis. "New approaches for processing and annotations of high-throughput metabolomic data obtained by mass spectrometry." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS359/document.
Повний текст джерелаMetabolomics is a phenotyping approach with promising prospects for the diagnosis and monitoring of several diseases. The most widely used observation technique in metabolomics is mass spectrometry (MS). Recent technological developments have significantly increased the size and complexity of data. This thesis focused on two bottlenecks in the processing of these data, the extraction of peaks from raw data and the annotation of MS/MS spectra. The first part of the thesis focused on the development of a new peak detection algorithm for Flow Injection Analysis (FIA) data, a high-throughput metabolomics technique. A model derived from the physics of the mass spectrometer taking into account the saturation of the instrument has been proposed. This model includes a peak common to all metabolites and a specific saturation phenomenon for each ion. This model has made it possible to create a workflow that estimates the common peak on well-behaved signals, then uses it to perform matched filtration on all signals. Its effectiveness on real data has been studied and it has been shown that proFIA is superior to existing algorithms, has good reproducibility and is very close to manual measurements made by an expert on several types of devices. The second part of this thesis focused on the development of a tool for detecting the structural similarities of a set of fragmentation spectra. To do this, a new graphical representation has been proposed, which does not require the metabolite formula. The graphs are also a natural representation of MS/MS spectra. Some properties of these graphs have then made it possible to create an efficient algorithm for detecting frequent subgraphs (FSM) based on the generation of trees covering graphs. This tool has been tested on two different data sets and has proven its speed and interpretability compared to state-of-the-art algorithms. These two algorithms have been implemented in R, proFIA and mineMS2 packages available to the community
Emanuele, Vincent A. II. "Advancements in high throughput protein profiling using surface enhanced laser desorption/ionization time of flight mass spectrometry." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/37287.
Повний текст джерелаSong, Wei. "MASS SPECTROMETRY-BASED HIGH THROUGHPUT APPROACH FOR IDENTIFICATION OF MOLECULAR MODIFICATION OF OXIDATIVE PROCESS IN RESPIRATORY." Cleveland State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=csu1226685494.
Повний текст джерелаLobue, Peter. "Towards the Parallel, Accurate, and High-throughput Mapping of RNA Modifications by Liquid Chromatography Tandem Mass Spectrometry." University of Cincinnati / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1595005836099446.
Повний текст джерелаHoward, James W. "The development of mass spectrometry-based methodologies for the high throughput quantitation of peptides in biological matrices." Thesis, Loughborough University, 2018. https://dspace.lboro.ac.uk/2134/32454.
Повний текст джерелаDevenport, Neil A. "Novel methods for the rapid and selective analysis of biological samples using hyphenated ion mobility-mass spectrometry with ambient ionization." Thesis, Loughborough University, 2014. https://dspace.lboro.ac.uk/2134/14287.
Повний текст джерелаBlacquiere, Johanna M. "New Directions in Catalyst Design and Interrogation: Applications in Dinitrogen Activation and Olefin Metathesis." Thèse, Université d'Ottawa / University of Ottawa, 2011. http://hdl.handle.net/10393/19968.
Повний текст джерелаSpiegel, Christopher [Verfasser], Kai [Gutachter] Simons, and Daniel [Gutachter] Müller. "Development of a high-throughput shotgun-mass spectrometry method for qualitative and quantitative analysis of major mammalian brain gangliosides / Christopher Spiegel ; Gutachter: Kai Simons, Daniel Müller." Dresden : Technische Universität Dresden, 2020. http://d-nb.info/1227832923/34.
Повний текст джерелаvan, Oosten Luuk Nico [Verfasser], and Christian D. [Akademischer Betreuer] Klein. "A novel high-throughput and label-free phenotypic drug screening approach: MALDI-TOF mass spectrometry combined with machine learning strategies / Luuk Nico van Oosten ; Betreuer: Christian D. Klein." Heidelberg : Universitätsbibliothek Heidelberg, 2020. http://d-nb.info/1211820904/34.
Повний текст джерелаSjödahl, Johan. "Miniaturized Techniques for Protein Analysis." Doctoral thesis, KTH, Chemistry, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3802.
Повний текст джерелаProteins are a highly diversified group of molecules, andfor their study, advanced analytical tools are required. Inparticular, a need for high-throughput techniques has emergedin order to enable the characterization of large sets ofproteins. In this thesis, improved techniques for proteinseparations as well as new tools for the mass spectrometricanalysis of proteins are described.
In the work, presented in the first part of the thesis, arefined extract containing proteases from Antarctic krill (Euphausia superba) was separated and characterized bymeans of capillary electrophoresis (CE) and mass spectrometry(MS). Tailored CE separations of the krill extract revealed thepresence of approximately 50 components. In addition, adetailed CE and MS analysis of fractions, containing individualkrill proteases has been carried out. Trypsin-like proteasesfrom krill exhibited a 12-fold and a 60-fold higher digestionefficiency at 37 °C and 2 °C respectively compared todigests performed with bovine trypsin. Furthermore, thecleavage specificity of the trypsin-like proteases wasstudied.
In the last part of the thesis, novel concepts forchip-based nanoelectrospray (nanoESI) and matrix-assisted laserdesorption/ionization (MALDI) mass spectrometry are described.First, a micromachined silicon chip with a two-dimensionalmatrix of out-ofplane nanoESI needles for high-throughputanalysis was fabricated. A two-fold improvement insignal-to-noise reproducibility was obtained. Second, achip-based target for MALDI was developed, which featured pairsof elevated 50×50 µm anchors in close proximity. Theanchors were individually addressable with sample solution. Theminiaturized sample preparations at close distance to eachother allowed a simultaneous ionization of a physicallyseparated sample and standard by one single laser pulse. Thisresulted in a twofold reduction of relative mass errors.Moreover, ion suppression of the analyte was significantlyreduced. The effective utilization of the sample resulted in adetection limit of ca 200 zeptomole of angiotensin I.
Key words:Proteins, peptides, proteases, Antarctickrill,Euphausia superba, capillary electrophoresis,fluorosurfactants, mass spectrometry, nanoelectrospray, ESI,MALDI, chip, high-throughput, reproducibility, sensitivity andmass accuracy.
Sanvitale, Caroline E. "Investigation of kinase activation in fibrodysplasia ossificans progressiva." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:3ac802e9-a864-4a0d-8e13-f21bcffc957d.
Повний текст джерелаAftab, Obaid. "Towards High-Throughput Phenotypic and Systemic Profiling of in vitro Growing Cell Populations using Label-Free Microscopy and Spectroscopy : Applications in Cancer Pharmacology." Doctoral thesis, Uppsala universitet, Cancerfarmakologi och beräkningsmedicin, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-234565.
Повний текст джерелаBourderioux, Matthieu. "Approches protéomiques pour l’analyse des exosomes de liquides biologiques pour la recherche de biomarqueurs." Thesis, Sorbonne Paris Cité, 2015. http://www.theses.fr/2015USPCB102/document.
Повний текст джерелаA biomarker is a molecule (or a cluster of molecule) which will reflect the occurrence of a pathological state, giving us the ability to detect a disease, to predict its severity or to assess drug efficiency. Biological fluids are the golden standards for biomarker research in human as they are routinely collected for patients’ follow-up and are less invasive than biopsies. During my PhD, I focused on exosomes that can be found in these biological fluids. Exosomes are nanovesicles with a diameter ranging between 30 and 100 nanometers. Exosomes are secreted by all cell types and harbor cytoplasmic and membranous proteins specific of their cells of origins. One of the major interest of exosomes enriched from biological fluids is that they represent a valuable source of biomarkers. They can be considered as a « liquid biopsy ». Their analysis could complete classical diagnosis and follow-up tools. In this project, we applied high resolution, high throughput proteomic techniques for exosomes analysis. We firstly focused on protein profiles in urinary exosomes in the context of two urinary tract diseases: cystinuria and kidney cancer. Cystinuria is an inherited autosomal recessive disease that is characterized by the formation of cystine stones in the kidneys. To date, there are no markers to predict the evolution toward end stage renal disease. We developed a method to prepare exosomes in order to reproducibly analyze their protein profiles. We applied this method to eight cystinuria patients and compared their profiles to those of ten healthy subjects. A panel of 38 differentially expressed proteins in patients were found and validated by western blots. We also applied this method to patients with clear cell renal cell carcinoma, for which invasive biopsies are necessary for clear diagnosis. We analyzed urinary exosomes form eight patients before and after nephrectomy. We were able to highlight 25 overexpressed proteins in patients’ exosomes. Eventually, the last part of my thesis was dedicated to the analysis of exosomes enriched from bronchoalveolar lavage fluid collected in cystic fibrosis patients, a disease that affects mostly the lungs. Bronchoalveolar lavage fluid exosomes analysis could give a new insight on the mechanisms of this disease. We compared protein profiles in exosomes from four cystic fibrosis patients and six asthmatic patients. The whole point of this work is to show that proteomic analysis of exosomes isolated from biological fluids could become a golden standard for the discovery of diagnosis or prognosis biomarkers
Duong-Thi, Minh-Dao. "Introducing weak affinity chromatography to drug discovery with focus on fragment screening." Doctoral thesis, Linnéuniversitetet, Institutionen för kemi och biomedicin (KOB), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-24642.
Повний текст джерелаOteng, Eugene K. "Discovery of a conserved Plasmodium antigen on the surface of malaria-infected red blood cells." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:592769fc-2628-4c6e-9a68-99060fb8c091.
Повний текст джерелаJouve, Thomas. "Étude des déterminants de la puissance statistique en spectrométrie de masse." Phd thesis, Université Claude Bernard - Lyon I, 2009. http://tel.archives-ouvertes.fr/tel-00635493.
Повний текст джерелаHabchi, Baninia. "Mise en évidence des perturbations métaboliques liées à l’exposition aux toxiques présents dans l’environnement ou l’aliment par spectrométrie de masse à ultra haute résolution FTMS combinée avec des outils chimiométriques." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLA032.
Повний текст джерелаPublic health monitoring involves evaluation of population exposure to environmental toxicants which can have an impact on their health. To do this, robust and high-throughput approaches are required to perform large scale analyses. Global approaches such as metabolomics which aim to reveal metabolic changes due to environmental stress or diseases seem to be the most appropriate approach. This multidisciplinary approach requires powerful analytical techniques such as mass spectrometry (MS) associated with statistical and chemometric data processing. It allows to detect general metabolic disruptions induced by a given physiological or pathological conditions. The studied samples can be injected either directly by the DIMS technique (direct introduction mass spectrometry) or following a chromatographic separation using GC/MS or LC/MS (gas or liquid chromatography / mass spectrometry). The DIMS approach leads to a significant reduction in analysis time, down to only a few minutes (usually less than 3 min). Additionally, in combination with Fourier transform mass spectrometers (DIFTMS), it provides very high mass resolving power and accurate mass measurements, as well as a wide dynamic range resulting in improved efficiency. Nevertheless, the DI-FTMS approach generates complex data containing several thousands of peaks. Processing such large data sets requires the development of dedicated chemometric and statistical tools to detect exposure biomarkers. Therefore, the objective of my work was to develop a rapid, highthroughput workflow, including the development of chemometric tools, in order to highlight metabolomic perturbations induced by exposure to toxicants. The first part of this work concerns the study of farmers professionally exposed to two pesticides. The DIMS approach was performed on an Orbitrap instrument and a new chemometric tool called Independent Component - Discriminant Analysis (IC-DA) was developed for supervised analysis of the DIMS data. The developed methodology was then applied to a larger number of samples corresponding to five types of exposure. In this later study, two analytical approaches DIMS and LC/MS were examined in order to validate the DIMS approach as well as the developed chemometric data analysis tool. In a second part of this work, the DIMS approach was applied to an instrument of higher performances, the FT-ICR (Fourier transform-ion cyclotron resonance) equipped with a dynamically harmonized cell in order to improve the quality of the DIMS data. A first study explored the effects of exposure of rats to different concentrations of pesticides. In a second step, the procedure was applied to a large number of samples (of approximately 500 individuals) to test the robustness of the approach. All this work demonstrated the feasibility and effectiveness of our high-throughput metabolomic approach combining the direct introduction (DIMS), the very high resolution detection and the chemometric tools. This approach could be very promising to perform large scale metabolic phenotyping such as in epidemiological studies
Largy, Eric. "Ciblage d’acides nucléiques G-quadruplexes : synthèse et développement de méthodes pour l’analyse et le criblage de ligands sélectifs multimodaux." Thesis, Paris 11, 2011. http://www.theses.fr/2011PA112257.
Повний текст джерелаThe aim of this thesis work was to study the interactions of small molecules with multiple structures of quadruplex DNA via i) the development and use of a high-throughput test for the analysis of ligand-quadruplex DNA interactions and screening of chemical libraries and ii) the preparation of compounds with multiple binding modes (stacking/groove, covalent/non-covalent, etc..) selective (quadruplex vs. duplex and intra-quadruplex) and possibly functionalized (biotin, fluorophore, etc.). The first part of the work was focused on the development of the G4-FID (G-quadruplex Intercalator Fluorescent Displacement) assay, which is a semi-quantitative method for evaluating the affinity and selectivity of small molecules for quadruplex DNA by displacing an off/on probe, the Thiazole Orange (TO). The test has been implemented successfully with microplate (HT-G4-FID). On the other hand, we have shown the importance of alternative fluorophores, TO-PRO-3 and Hoechst 33258, with complementary spectral characteristics. This method of analysis has also been successfully used for the identification of new selective ligands of quadruplex DNA and the identification of structure-activity relationships and structural selectivities. The second part of the work was devoted to the preparation and study of new DNA quadruplex ligands. These ligands possess particular characteristics either in their mode of interaction (grooves, coordination) or by their bifunctionality (biotinylated, fluorescent). We have prepared an acyclic polyheteroaryle quadruplex ligand (TOxaPy) with an unexpected selectivity for certain structures of quadruplex DNA. Furthermore, we showed that complexes of terpyridine derivatives can be tailored by changing the organic ligand and / or the metal in order to interact with quadruplex DNA by covalent and / or non-covalent interaction
Stoppe, Nancy de Castro 1963. "Prospecção de marcadores para o rastreamento de fontes de contaminação fecal em águas superficiais do Estado de São Paulo." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316933.
Повний текст джерелаTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-26T13:28:27Z (GMT). No. of bitstreams: 1 Stoppe_NancydeCastro_D.pdf: 4282286 bytes, checksum: f808229a92e702d707b4ebf6be210816 (MD5) Previous issue date: 2014
Resumo: A contaminação fecal dos corpos hídricos é uma das principais causas de doenças entéricas veiculadas pela água no mundo, sendo importante efetuar a vigilância da água, a qual é feita utilizando-se micro-organismos indicadores de contaminação fecal. No entanto, os métodos tradicionais de detecção não são capazes de identificar a fonte de contaminação fecal. Este trabalho teve como objetivo a prospecção de marcadores moleculares nos hospedeiros e sua detecção em amostras de água de modo a permitir sua utilização na identificação de fontes de contaminação fecal em águas superficiais no Estado de São Paulo. Duas abordagens dependentes de biblioteca e cultivo, a classificação por meio de grupos filogenéticos e a técnica de MALDI-TOF/MS, foram utilizadas com linhagens de E. coli isoladas de diferentes hospedeiros e rios e reservatórios. O sequenciamento da região V3 do gene 16S ribossomal foi selecionado como o método independente de biblioteca e cultivo em DNA extraído de amostras de fezes humanas e bovinas e amostras de água. Os grupos filogenéticos foram utilizados na classificação dos hospedeiros utilizando análise de correspondência, onde foram observados agrupamentos por hábitos alimentares. A classificação das linhagens de E. coli isoladas de rios e reservatórios, sugere que a prevalência do subgrupo A1, seguido do subgrupo B23 está associada com contaminação de origem humana, enquanto o grupo B1 com contaminação de origem animal e os subgrupos D1 e D2 mais relacionados com ambientes prístinos. Na utilização de uma métrica de análise de rede social, w-clique, na distribuição dos grupos filogenéticos foi observado o agrupamento dos locais de amostragem associados ao grau de poluição, sugerindo seu uso como uma ferramenta complementar na avaliação da qualidade da água. Foram analisados os perfis proteicos de linhagens de E. coli de hospedeiros e amostras ambientais pela técnica de MALDI-TOF/MS. Foram identificados biomarcadores hospedeiro-específicos e sugerem sua utilização como potencial ferramenta na identificação da origem do hospedeiro. Os resultados da validação desses marcadores com perfis proteicos de linhagens de E. coli isoladas de rios e reservatórios mostraram que as amostras de água apresentaram marcadores de diferentes hospedeiros, sugerindo que esses rios possuam fontes de contaminação fecal mistas. No sequenciamento da região V3 do gene 16S ribossomal de amostras de fezes (humanas e bovinas) e águas foram identificadas 4.296 unidades taxonômicas operacionais (OTUs) . A maior diversidade foi observada nas amostras de fezes bovinas e a menor na amostra de água de ambiente prístino. Firmicutes foi o grupo predominante nas amostras de fezes humanas, enquanto que nas fezes bovinas foram os Firmicutes e Bacteroidetes. Nas amostras de água, o filo mais abundante foi Proteobacteria. A rede de interação entre as OTUs encontradas nas amostras também mostrou que as amostras de fezes apresentaram maior diversidade e entre as amostras de água, aquela com poluição de origem humana foi a que apresentou maior diversidade. Houve identificação de biomarcadores pelo método LEfSe para humanos (Actinobacteria, Betaproteobacteria e Firmicutes) e bovinos: (Bacteroidetes, Tenericutes e Spirochaetes). Marcadores hospedeiro-específicos foram identificados, mas esses não foram encontrados nas amostras de água sugerindo que as ferramentas utilizadas não apresentam a resolução para identificar os marcadores nas amostras ambientais ou que a contaminação nos corpos hídricos é mista. Adicionalmente, como os marcadores hospedeiro-específicos são oriundos de micro-organismos não autóctones, estes poderiam sofrer os efeitos adversos do ambiente, como fatores físico-químicos e competição com os organismos nativos
Abstract: The fecal contamination of water resources is the main cause of enteric waterborne diseases all over the world. Traditional indicator methods used in the water microbiological quality assessment are not able to identify fecal contamination source. This work intended to prospect molecular markers in hosts and track them in water samples to identify pollution sources in surface waters in the São Paulo State, Brazil. Two library-dependent methods with E. coli strains isolated from different hosts and water samples were used, a genotypic typing method (E. coli phylogenetic groups) and a phenotypic typing method (MALDI-TOF/MS). A library-independent method using 454 pyrosequencing of hypervariable16S rRNA gene V3 region was used in DNA from feces and water samples. Phylogenetic groups were used as a tool in host classification and correspondence analysis showed feeding habits clusters. The classification of environmental samples revealed higher frequencies of subgroups A1 and B23 in rivers impacted by human pollution sources, while subgroups D1 and D2 were associated with pristine sites, and subgroup B1 with domestic animal sources, indicating their use as a first screening for pollution source identification. A simple classification is proposed based on phylogenetic subgroup distribution using the w-clique metric, enabling differentiation of polluted and unpolluted sites. Protein profiles of E. coli strains isolated from host and water samples were analyzed by MALDI-TOF/MS. Specific host biomarkers were identified and their use was indicated as a potential tool for the source tracking. Validation with E. coli strains isolated from rivers and reservoirs showed that water samples presented markers from different hosts, suggesting these rivers have mixed sources of fecal contamination. Sequencing of the 16S rRNA V3 region in stool samples (human and bovine) and water showed 4296 operational taxonomic units (OTUs). The greatest diversity was observed in samples of cattle feces and the smallest one in the pristine water sample. Firmicutes was the predominant group in samples of human feces, while in the most common bovine feces are the Firmicutes and Bacteroidetes. The interaction network showed that the stool samples had the greatest diversity and, among them, the water sample with human pollution source showed the highest diversity. The LEfSe method was used to identify host biomarkers. As human biomarkers, Actinobacteria, Betaproteobacteria and Firmicutes were identified and for cattle the potential markers are Bacteroidetes, Tenericutes and Spirochaetes. Host-specific markers were identified, but they were not found in water samples suggesting that the used tools either do not have the resolution to identify markers in environmental samples or contamination in water bodies is mixed. Additionally, as the host-specific markers were isolated from non-autochthonous micro-organisms, they could be affected by the environmental adverse effects such as physical-chemical factors and competition with native organisms
Doutorado
Microbiologia
Doutora em Genética e Biologia Molecular
(8086205), David L. Logsdon. "HIGH-THROUGHPUT ORGANIC REACTION SCREENING USING DESORPTION ELECTROSPRAY IONIZATION MASS SPECTROMETRY." Thesis, 2019.
Знайти повний текст джерелаLeDuc, Richard D. "Bioinformatics of high throughput proteomics using tandem mass spectrometry of intact proteins /." 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3290288.
Повний текст джерелаSource: Dissertation Abstracts International, Volume: 68-11, Section: B, page: 7046. Adviser: Gustavo Caetano-Anolles. Includes bibliographical references (leaves 117-129) Available on microfilm from Pro Quest Information and Learning.
Boltz, Stacey A. "High throughput proteomic studies using fourier transform ion cyclotron resonance mass spectrometry." 2003. http://purl.galileo.usg.edu/uga%5Fetd/boltz%5Fstacey%5Fa%5F200305%5Fms.
Повний текст джерелаChen, Ming-Shou, and 陳明壽. "Biomarker discovery on high-throughput and high-resolution mass spectrometry oral cancer data using statistical methods." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/56cbf4.
Повний текст джерела國立中央大學
資訊工程研究所
94
Due to the high death rate in advanced stage diseases, the diagnosis of early-stage cancer is needed for public health. Recent advances in the biotechnology of high-throughput and high-resolution MALDI-TOF mass spectrometry (MS) has made such diagnosis possible (Petricoin and Liotta 2003). From then on, high-resolution mass spectrometers are increasingly used for disease classification and therapeutic guidance. Due to instrument resolution and/or instrument calibration, the mass spectrometry (MS) data may be poor in quality. The problem makes it difficult and time consuming to trace each spectrum with thousands of features for biomarkers. We proposed a region-based alignment method to deal with peak shifting problem and applied statistical method to select most discriminatory peaks as biomarker candidates. Finally, we test our methodology on the oral cancer dataset from CHANG GUNG UNIVERSITY in Taiwan.
(7900775), Harrison S. Ewan. "HIGH THROUGHPUT EXPERIMENTATION WITH DESORPTION ELECTROSPRAY IONIZATION MASS SPECTROMETRY TO GUIDE CONTINUOUS-FLOW SYNTHESIS." Thesis, 2019.
Знайти повний текст джерела"The development of high-throughput mass spectrometric methods for the qualitative and quantitative analysis of diquaternary ammonium gemini surfactants." Thesis, 2013. http://hdl.handle.net/10388/ETD-2013-11-1289.
Повний текст джерелаChang, Yuan-Jhe, and 張元哲. "Method Development of the New Generation Hair Testing by High Throughput Liquid Chromatography Tandem Mass Spectrometry." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/52018740213623060734.
Повний текст джерела中山醫學大學
醫學研究所
102
Compared to blood and urine, hair is unique in that it could determine the time period of chemical exposure after several months to years. The main purpose of this study is to develop high throughput and sensitivity analysis method of hair testing. Firstly, we developed a fast microwave-assisted extraction method for reducing the incubation time of abused drug release from human hair. In addition, extraction-free sample preparation and liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis are evaluated for improving throughput of experiments. Secondly, a high sensitive method for detecting trace amount THC-COOH in hair by using strategy of simple and fast chemical derivatization with dansyl chloride coupled with LC–MS/MS. Thirdly, a stable isotope dilution liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed to detect the metabolites of human DEHP exposure in hair. We investigated the incubation conditions for improving the releae efficiency of metabolites from hair. Furthermore, an automated on line solid-phase extraction (On-line SPE) coupled with LC–MS/MS system was developed for reducing experiment time and increasing the high-throughput of analysis. In particular, the authentic hair specimens were determined and perfromed of segmental hair analysis for proving the practicability of assessing DEHP exposure in human hair. Consequently, an LC–MS/MS method was developed and utilized to authentic hair that could be successfully determined of parent compound of clobenzorex to discriminate the truly condition of clobenzorex used while not AMP.
Lin, Shiau-Pei, and 林曉珮. "High Throughput Screening for Zearalenone by Matrix-Assisted Laser Desorption/Inoization Time-of -Flight Mass Spectrometry." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/24451532426214919195.
Повний текст джерела朝陽科技大學
應用化學系碩士班
98
Zearalenone (ZEA) is one of the mycotoxins which are the secondary metabolites of fungi. It is found in corn, wheat, barley and oats. In Taiwan, the official analysis method for ZEA is using disposable immunoaffinity column coupling with high performance liquid chromatography (HPLC) which has the detection limit of 10 ppb. However, the modern preservation of grain has reduced the contamination of ZEA in corps. The cost of the specific column and the time consuming are not good for high throughput analysis. If there is a low cost and high throughput screening methods to filter out most uncontaminated samples, a lot of time consuming and cost would be avoided. Here we report a low cost and high throughput screening method for the analysis of ZEA using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). This method includes two parts. First, the sample preparation using celite to remove sample matrix is simple and low cost. Second, without general matrix for co-crystal with analyte, aluminum foil is used to obtain low noise analyte spectra in low mass range. The cost of sample preparation for each sample is less than NT$ 10.0 dollars, and analysis time for each sample is only 1 min or less, detection limit can reach to 10 ppb which is comparable with the official method.
(9137873), Zhuoer Xie. "Accelerating the Throughput of Mass Spectrometry Analysis by Advanced Workflow and Instrumentation." Thesis, 2020.
Знайти повний текст джерелаThe exploratory profiling and quantitative bioassays of lipids, small metabolites, and peptides have always been challenging tasks. The most popular instrument platform deployed to solve these problems is chromatography coupled with mass spectrometry. However, it requires large amounts of instrument time, intensive labor, and frequent maintenance, and usually produces results with bias. Thus, the pace of exploratory research is one of poor efficacy and low throughput. The work in this dissertation provides two practical tactics to address these problems. The first solution is multiple reaction monitoring profiling (MRM-profiling), a new concept intended to shift the exploratory research from current identification-centered metabolomics and lipidomics to functional group screening by taking advantage of precursor ion scan and product ion scan. It is also demonstrated that MRM-profiling is capable of quantifying the relative amount of lipids within the same subclass. Besides, an application of the whole workflow to investigate the strain-level differences of bacteria is described. The results have zeroed in on several potential lipid biomarkers and corresponding MRM transitions. The second strategy is aimed to increase the throughput of targeted bioassays by conducting induced nanoelectrospray ionization (nESI) in batch mode. A novel prototype instrument named "Dip-and-Go" system is presented. Characterization of its ability to carry out reaction screening and bioassays exhibits the versatility of the system. The distinct electrophoretic cleaning mechanism contributes to the removal of salt during ionization, which assures the accuracy of measurement.
Wang, Chin-Hsiung, and 王慶雄. "Solid phase microextraction combined with ambient ionization mass spectrometry for high-throughput pharmacokinetic analysis of human plasma." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/57j7pq.
Повний текст джерела國立中山大學
化學系研究所
107
The content of this thesis mainly includes the development of solid phase microextraction combined with ambient mass spectrometry for high-throughput analysis of drug for pharmacokinetic studies. Traditionally, liquid or gas chromatography tandem mass spectrometry requires lengthy and laborious extraction and concentration process so that it is prone to misconducts and experimental errors. Therefore a simple and high-throughput analytical approach is deemed necessary. In this thesis, the solid phase microextraction technique combined thermal desorption electrospray ionization mass spectrometry (SPME-TD-ESI/MS) approach is used to rapidly determine the concentration of drugs in pharmacokinetics samples. Polar, relatively non-polar, and combine of both adsorption materials SPME fiber were explored for direct immersion solid phase microextraction thermal desorption electrospray ionization mass spectrometry approach. In addition, a new developed in-tip SPME device was firstly disclosed and proved to use 1 μL plasma sample for each analysis. Experimental results shown that the average errors were less than 20% compared to LC-MS/MS method. Lower limit of quantification as low as 0.2 ng mL-1 and linear regression coefficient (r) values were above 0.995. The SPME extraction and instrument detection time was as short as 2.5 min. In overall, experimental results show that SPME-TD-ESI/MS approach is not only suitable for high-throughput analysis of pharmacokinetic samples. Polar and non-polar SPME fiber could also be flexibly combined for simultaneously extracting analytes of different polarity. The new design of in-tip SPME pushes down the sample volume to 1 μL plasma for each analysis greatly enhances its applicability in pharmacokinetics study especially in small animal models.
Wall, Mark James. "Towards the Development of a Proteomics Workflow for High-throughput Protein Biomarker Discovery." 2010. http://hdl.handle.net/10222/12840.
Повний текст джерелаZhou, Ao. "Characterizing alternative splicing and long non-coding RNA with high-throughput sequencing technology." Diss., 2018. http://hdl.handle.net/1805/17771.
Повний текст джерелаSeveral experimental methods has been developed for the study of the central dogma since late 20th century. Protein mass spectrometry and next generation sequencing (including DNA-Seq and RNA-Seq) forms a triangle of experimental methods, corresponding to the three vertices of the central dogma, i.e., DNA, RNA and protein. Numerous RNA sequencing and protein mass spectrometry experiments has been carried out in attempt to understand how the expression change of known genes affect biological functions in various of organisms, however, it has been once overlooked that the result data of these experiments are in fact holograms which also reveals other delicate biological mechanisms, such as RNA splicing and the expression of long non-coding RNAs. In this dissertation, we carried out five studies based on high-throughput sequencing data, in an attempt to understand how RNA splicing and differential expression of long non-coding RNAs is associated biological functions. In the first two studies, we identified and characterized 197 stimulant induced and 477 developmentally regulated alternative splicing events from RNA sequencing data. In the third study, we introduced a method for identifying novel alternative splicing events that were never documented. In the fourth study, we introduced a method for identifying known and novel RNA splicing junctions from protein mass spectrometry data. In the fifth study, we introduced a method for identifying long non-coding RNAs from poly-A selected RNA sequencing data. Taking advantage of these methods, we turned RNA sequencing and protein mass spectrometry data into an information gold mine of splicing and long non-coding RNA activities.
2019-05-06
(6618998), Zinia Jaman. "HIGH THROUGHPUT EXPERIMENTATION AS A GUIDE TO THE CONTINUOUS FLOW SYNTHESIS OF ACTIVE PHARMACEUTICAL INGREDIENTS." Thesis, 2020.
Знайти повний текст джерелаContinuous flow chemistry for organic synthesis is an emerging technique in academia and industry because of its exceptional heat and mass transfer ability and, in turn, higher productivity in smaller reactor volumes. Preparative electrospray (ES) is a technique that exploits reactions in charged microdroplets that seeks to accelerate chemical synthesis. In Chapter 2, the flow synthesis of atropine, a drug which is included in the WHO list of essential of medicines and currently in shortage according to the U.S Food and Drug Administration (FDA)is reported.The two steps of atropine synthesis were initially optimized separately and then continuously synthesized using two microfluidic chips under individually optimized condition.The telescoped continuous-flow microfluidics experiment gave a 55% conversion with an average of 34% yield in 8 min residence time. In Chapter 3, a robotic HTE technique to execute reactions in 96-well arrays was coupled with fast MS analysis. Palladium-catalyzed Suzuki-Miyaura (S-M) cross-coupling reactions were screened in this system and a heat map was generated to identify the best reaction condition for downstream scale up in continuous flow.
In Chapter 4, an inexpensive and rapid synthesis of an old anticancer drug, lomustine,was synthesized. Using only four inexpensive commercially available starting materials and a total residence time of 9 min, lomustine was prepared via a linear sequence of two chemical reactions performed separately in two telescoped flow reactors. Sequential offline extraction and filtration resulted in 63% overall yield of pure lomustine at a production rate of 110 mg/h. The primary advantage of this approach lies in the rapid manufacture of lomustine with two telescoped steps to avoid isolation and purification of a labile intermediate, thereby decreasing the production cost significantly. A high throughput reaction screening approach based on desorption electrospray ionization mass spectrometry (DESI-MS) is described in Chapter 4 and 5 for finding the heat-map from a set of reaction conditions. DESI-MS is used to quickly explore a large number of reaction conditions and guide the efficient translation of optimized conditions to continuous flow synthesis that potentially accelerate the process of reaction optimization and discovery. Chapter 5 described HTE ofSNAr reactions using DESI-MS and bulk techniques with 1536 unique reaction conditions explored using both in DESI-MS and bulk reactors. The hotspots from the HTE screening effort were validated using a microfluidic system that confirmed the conditions as true positives or true.
Hopper, Erin D. "Development and Application of a Mass Spectrometry-Based Assay for the High Throughput Analysis of Protein-Ligand Binding." Diss., 2009. http://hdl.handle.net/10161/1117.
Повний текст джерелаMany of the biological roles of proteins are modulated through protein-ligand interactions, making proteins important targets for drug therapies and diagnostic imaging probes. The discovery of novel ligands for a protein of interest often relies on the use of high throughput screening (HTS) technologies designed to detect protein-ligand binding. The basis of one such technology is a recently reported mass spectrometry-based assay termed SUPREX (stability of unpurified proteins from rates of H/D exchange). SUPREX is a technique that uses H/D exchange and MALDI-mass spectrometry for the measurement of protein stabilities and protein-ligand binding affinities. The single-point SUPREX assay is an abbreviated form of SUPREX that is capable of detecting protein-ligand interactions in a high throughput manner by exploiting the change in protein stability that occurs upon ligand binding.
This work is focused on the development and application of high throughput SUPREX protocols for the detection of protein-ligand binding. The first step in this process was to explore the scope of SUPREX for the analysis of non-two-state proteins to determine whether this large subset of proteins would be amenable to SUPREX analyses. Studies conducted on two model proteins, Bcl-xL and alanine:glyoxylate aminotransferase, indicate that SUPREX can be used to detect and quantify the strength of protein-ligand binding interactions in non-two-state proteins.
The throughput and efficiency of a high throughput SUPREX protocol (i.e., single-point SUPREX) was also evaluated in this work. As part of this evaluation, cyclophilin A, a protein target of diagnostic and therapeutic significance, was screened against the 880-member Prestwick Chemical Library to identify novel ligands that might be useful as therapeutics or imaging agents for lung cancer. This screening not only established the analytical parameters of the assay, but it revealed a limitation of the technique: the efficiency of the assay is highly dependent on the precision of each mass measurement, which generally decreases as protein size increases.
To overcome this limitation and improve the efficiency and generality of the assay, a new SUPREX protocol was developed that incorporated a protease digestion step into the single-point SUPREX protocol. This new protocol was tested on two model proteins, cyclophilin A and alanine:glyoxylate aminotransferase, and was found to result in a significant improvement in the efficiency of the SUPREX assay in HTS applications. This body of work resulted in advancements in the use of SUPREX for high throughput applications and laid the groundwork for future HTS campaigns on target proteins of medical significance.
Dissertation
(10971108), Yangjie Li. "REACTION ACCELERATION AT INTERFACES STUDIED BY MASS SPECTROMETRY." Thesis, 2021.
Знайти повний текст джерелаVarious organic reactions, including important synthetic reactions involving C–C, C–N, and C–O bond formation as well as reactions of biomolecules, are known to be accelerated when the reagents are present in confined volumes such as sprayed or levitated microdroplets or thin films. This phenomenon of reaction acceleration and the key role of interfaces played in it are of intrinsic interest and potentially of practical value as a simple, rapid method of performing small-scale synthesis. This dissertation has three focusing subtopics in the field of reaction acceleration: (1) application of reaction acceleration in levitated droplets and mass spectrometry to accelerate the reaction-analysis workflow of forced degradation of pharmaceuticals at small scale; (2) fundamental understanding of mechanisms of accelerated reactions at air/solution interfaces; (3) discovery the use of glass particles as a `green' heterogeneous catalysts in solutions and systematical study of solid(glass)/solution interfacial reaction acceleration as a superbase for synthesis and degradation using high-throughput screening.
Reaction acceleration in confined volumes could enhance analytical methods in industrial chemistry. Forced degradation is critical to probe the stabilities and chemical reactivities of therapeutics. Typically performed in bulk followed by LC-MS analysis, this traditional workflow of reaction/analysis sequence usually requires several days to form and measure desirable amount of degradants. I developed a new method to study chemical degradation in a shorter time frame in order to speed up both drug discovery and the drug development process. Using the Leidenfrost effect, I was able to study, over the course of seconds, degradation in levitated microdroplets over a metal dice. This two-minute reaction/analysis workflow allows major degradation pathways of both small molecules and therapeutic peptides to be studied. The reactions studied include deamidation, disulfide bond cleavage, ether cleavage, dehydration, hydrolysis, and oxidation. The method uses microdroplets as nano-reactors and only require a minimal amount of therapeutics per stress condition and the desirable amount of degradant can be readily generated in seconds by adjusting the droplet levitation time, which is highly advantageous both in the discovery and development phase. Built on my research, microdroplets can potentially be applied in therapeutics discovery and development to rapidly screen stability of therapeutics and to screen the effects of excipients in enhancing formulation stabilities.
My research also advanced the fundamental understanding of reaction acceleration by disentangles the factors controlling reaction rates in microdroplet reactions using constant-volume levitated droplets and Katritzky transamination as a model. The large surface-to-volume ratios of these systems results in a major contribution from reactions at the air/solution interface where reaction rates are increased. Systems with higher surface-active reactants are subject to greater acceleration, particularly at lower concentrations and higher surface-to-volume ratios. These results highlight the key role that air/solution air/solution interfaces play in Katritzky reaction acceleration. They are also consistent with the view that reaction increased rate constant is at least in part due to limited solvation of reagents at the interface.
While reaction acceleration at air/solution interfaces has been well known in microdroplets, reaction acceleration at solid/solution interfaces appears to be a new phenomenon. The Katritzky reaction in bulk solution at room temperature is accelerated significantly by the surface of a glass container compared to a plastic container. Remarkably, the reaction rate is increased by more than two orders of magnitude upon the addition of glass particles with the rate increasing linearly with increasing amounts of glass. A similar phenomenon is observed when glass particles are added to levitated droplets, where large acceleration factors are seen. Evidence shows that glass acts as a ‘green’ heterogeneous catalyst: it participates as a base in the deprotonation step and is recovered unchanged from the reaction mixture.
Subsequent to this study, we have systematically explored the solid/solution interfacial acceleration phenomena using our latest generation of a high-throughput screening system which is capable of screening thousands of organic reactions in a single day. Using desorption electrospray ionization mass spectrometry (DESI-MS) for automated analysis, we have found that glass promotes not only organic reactions without organic catalysts but also reactions of biomolecules without enzymes. Such reactions include Knoevenagel condensation, imine formation, elimination of hydrogen halide, ester hydrolysis and/or transesterification of acetylcholine and phospholipids, as well as oxidation of glutathione. Glass has been used as a general `green' and powerful heterogeneous catalyst.
Huang, Jing-Yi, and 黃靜怡. "Application of Liquid Chromatography-Tandem Mass Spectrometry for High Throughput Screening of Sedative-Hypnotics and Abused Drugs in Urine." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/dcga4k.
Повний текст джерела國立中興大學
化學系所
99
The people who has insomnia is increasing rapidly because of working pressure and fast pace of life. Prescription drugs used specifically for improving sleeping are called sedative hypnotics. These drugs include benzodiazepines and non-benzodiazepines such as zolpidem. People who use drugs for pain relief may become dependent and drug abuse can lead to drug dependence or addiction. Ketamine, like benzodiazepines, is a central nervous system depressant. It is primarily used for the induction and maintenance of general anesthesia, usually in combination with a sedative, and is a quick acting drug used in human and veterinary medicine. Ketamine has over the past few years been thought of as a “club drug”, and often used with sedative hypnotics. These drugs can cause dream-like states and hallucinations. Since drug abuse that alters human behavior and leads to crimes has become a serious problem throughout the world, it is important to develop a fast and accurate method to determine abused drugs. The aim of this study is to develop a rapid micro solid phase extraction (μSPE) combined with liquid chromatography/ tandem mass spectrometry (LC/MS/MS) for simultaneous determination 14 drugs of the third and fourth controlled drugs and metabolites in urine. The 14 target analytes are flunitrazepam, nimetazepam, triazolam, diazepam, estazolam, alprazolam midazolam, lorazepam, zaleplon, zopiclone, zolpidem, ketamine and two metabolits of ketamine. The highly selective reaction monitoring (H-SRM) scan mode was used in this study to improve selectivity and sensitivity. The linearity ranges were between 0.5~500 ng/mL with coefficients of determination (R2) above 0.9916 for 14 drugs. The limit of detection (LOD) ranged from 0.15~6 ng/mL ng/mL. The average recoveries at 10 ng/mL were 78.0~103 % and the relative standard deviations (RSD%) were 7.0~18.2 %, precisions of interday and intraday were 3.7~15.1 % and 5.6~17.9 % respectively. Thus, the established method should be useful in determination of abused drugs in real urine samples.
Maděra, Milan. "Development of Methods for High-Throughput Enrichment of Glycoproteins and Glycopeptides Employing Multiple Lectin Affinity Chromatography/Tandem Mass Spectrometry." Doctoral thesis, 2006. http://www.nusl.cz/ntk/nusl-374322.
Повний текст джерелаSpiegel, Christopher. "Development of a high-throughput shotgun-mass spectrometry method for qualitative and quantitative analysis of major mammalian brain gangliosides." 2019. https://tud.qucosa.de/id/qucosa%3A72379.
Повний текст джерелаMirnaghi, Fatemeh Sadat. "High-throughput analysis of biological fluids using 96-blade (thin-film) solid phase microextraction system." Thesis, 2012. http://hdl.handle.net/10012/7175.
Повний текст джерела"High-throughput quantitative profiling of serum N-glycome by MALDI-TOF mass spectrometry and N-glycomic fingerprint of liver fibrosis." 2008. http://library.cuhk.edu.hk/record=b5893558.
Повний текст джерелаThesis (M.Phil.)--Chinese University of Hong Kong, 2008.
Includes bibliographical references (leaves 169-192).
Abstracts in English and Chinese.
Chapter 1. --- Abstract --- p.ii
English --- p.ii
Chinese --- p.v
Chapter 2. --- Acknowledgments --- p.vii
Chapter 3. --- Abbreviations and N-glycan representation --- p.viii
Chapter 4. --- Introduction --- p.1
Chapter 5. --- Review of Literatures --- p.2
Chapter 5.1. --- Introduction to Liver Fibrosis --- p.2
Chapter 5.1.1. --- Pathogenesis of Liver Fibrosis --- p.2
Chapter 5.1.2. --- Changes of liver architecture - basis of liver fibrosis diagnosis --- p.4
Chapter 5.2. --- Current Diagnosis of Liver Fibrosis - from Biopsy Examination to Serum Test --- p.5
Chapter 5.3. --- Glycomics and its Potential as Biomarkers --- p.9
Chapter 5.3.1. --- Overview of Biochemical and Functional Characteristics of Glycan --- p.13
Chapter 5.3.2. --- N-linked and O-linked Glycosylations - A Valuable Source of Biomarkers --- p.15
Chapter 5.3.3. --- Glycomics 一 An Uprising Approach for Biomarker Discovery --- p.17
Chapter 5.3.4. --- Human Proteome Organisation Human Disease Glycomics/Proteome Initiative --- p.19
Chapter 5.3.5. --- Recent Applications of Glycomics to Biomarker Discovery --- p.20
Chapter 5.4. --- Current Technologies for Glycomic Study --- p.22
Chapter 5.4.1. --- MALDI-TOF MS --- p.22
Chapter 5.4.2. --- Lectin Microarray --- p.25
Chapter 5.4.3. --- Liquid Chromatography --- p.27
Chapter 5.4.4. --- Capillary Electrophoresis --- p.29
Chapter 5.4.5. --- Quantitative Profiling of Tissue Glycome --- p.31
Chapter 6 --- Project Rationales and Objectives --- p.36
Chapter 7 --- Section 1: Methodology Development of Quantitative N- glycomic Profiling --- p.37
Chapter 1. --- Introduction --- p.37
Chapter 2. --- Method and Materials --- p.39
Chapter 3. --- Results --- p.46
Chapter 4. --- Discussion --- p.65
Chapter 5. --- Conclusion --- p.71
Chapter 8. --- Section 2: Serum N-glycomic Profile as Biomarker for Liver Fibrosis 一 Pilot Study --- p.73
Chapter 1. --- Introduction --- p.73
Chapter 2. --- Method and Materials --- p.75
Chapter 3. --- Results --- p.79
Chapter 4. --- Discussion --- p.86
Chapter 5. --- Conclusion --- p.94
Chapter 9. --- Section 3: Serum N-glycomic Profile as Biomarker for Liver Fibrosis -Verification Study --- p.96
Chapter 1. --- Introduction --- p.96
Chapter 2. --- Method and Materials --- p.98
Chapter 3. --- Results --- p.104
Chapter 4. --- Discussion --- p.137
Chapter 5. --- Conclusion --- p.152
Chapter 10. --- General Discussion --- p.153
Chapter 11. --- Conclusion --- p.167
Chapter 12. --- Original Data --- p.168
Chapter 13. --- References --- p.169
Chapter 14. --- Publications --- p.196
Lin, Jing-yun, and 林靜芸. "The Development of High Throughput Screening Technique for Fumonisins by Matrix-Assisted Laser Desorption/Inoization Time-of-Flight Mass Spectrometry." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/58678809244737949069.
Повний текст джерела朝陽科技大學
應用化學系碩士班
99
Fumonisins are secondary metabolites of Fusarium moniliforme, mainly in corn and corn-based products. It may cause flu-like symptoms on pig and result in wrong treatment. Moreover, fumonisins are associated with human esophageal cancer. Fumonisin B1 (FB1) is the most toxic and most natural abundant type (70%) in Fumonisins. The official analysis method for FB1 is using disposable immunoaffinity column (NT$≧300) for extraction then following derivatization before HPLC-FLD measurement. However, the cost of the specific column and the time consuming are not good for high throughput analysis, especially for the non-contaminated samples. If there is a low cost and high throughput screening methods to filter out most uncontaminated samples, a lot of time consuming and cost would be avoided. Thus, we develop a low-cost, high sensitivity screening method for FB1 by MALDI-TOF MS to achieve this goal. In this method, the pretreatment just need solvent (MeOH/H2O) extraction following centrifugation, then detected by MALDI-TOF/MS using α-CHCA as the matrix and Cs+ to increase sensitivity to 1 ppb. The detection limit would be only 1 ppm without Cs+. The cost of sample preparation for each sample is less than NT$ 10.0 dollars and analysis time for each sample is only 1 min or less. This is in line with current environmental protection concept of green chemistry.
Hu, Li-Wen, and 胡瓈文. "The Development of High Throughput Screening Technique for Citrinin by Matrix-Assisted Laser Desorption/Inoization Time-of -Flight Mass Spectrometry." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/73564751354971655339.
Повний текст джерела朝陽科技大學
應用化學系
102
Citrinin (Citrinin, CIT) , a kind of mycotoxins with teratogenic toxicity and harmful to kidney and liver, is a metabolite of Monascus fermentation during generating red pigment. Since more and more health-related Monascus fermentation products are introduced to the market, the risk of citrinin contaiminations may increase. Therefore , I develope a low-cost, and rapid screening method to detect CIT by using matrix-assisted laser desorption ionization / time of flight mass spectrometry (MALDI-TOF MS) . On this method, the pretreatment process requires only about 2 mL solvent to extract the sample centrifuged, the supernatant can be detected directly by MALDI-TOF/MS. When using α-CHCA as matrix, the detection limit is 1 ppm for standard, after adding α-cyclodextrin in the matrix, the limitation of detection can be improved 10 times to the 100 ppb. In the real specimen of wheat and red yeast pancake the detection limit can reach to 200 ppb ; for red yeast rice and red yeast capsules the detection limit can reach to 1 ppm. This method requires very low cost (NT ≦ 10) for each sample and the detection time is less than one minute. By this method, I may avoid unnecessary waste of materials and time consuming. This is in line with current environmental protection concept of green chemistry.
Lin, Mei-wun, and 林美文. "The Development of High Throughput Screening Technique for Deoxynivalenol by Matrix-Assisted Laser Desorption/Inoization Time-of -Flight Mass Spectrometry." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/45326567672230099451.
Повний текст джерела朝陽科技大學
應用化學系碩士班
97
Deoxynivalenol (DON or vomitoxin) is a mycotoxin and is one of the most common and abundant naturally occurring trichothecenes found in corn, wheat, and barley. Conventional analysis methods in the sample preparation and detection process is cost and time consuming, and therefore not suitable for high throughput screening. In Taiwan, the official analysis method for Deoxynivalenol is using immunoaffinity column coupling with high performance liquid chromatography (HPLC) which has the detection limit of 60 ppb. The cost of the specific column and the time consuming are not good for high throughput analysis. If there is a low cost and high throughput screening methods to filter out most uncontaminated samples, a lot of time consuming and cost would be avoided. Here we report a low cost and high throughput screening method for the analysis of Deoxynivalenol using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). First, This method includes two part, sample preparation method based on celite is simple and low cost. Second, high throughput of the MALDI-TOF MS supply a detection. An ionic liquid matrix (Et3N-a-CHCA) is used to obtain low matrix noise mass spectra for low molecular weight Deoxynivalenol and the Deoxynivalenol signal was enhanced by adding KCl via K+ cationization. The method of sample preparation cost for each sample is NT$ 10.0 dollars, and a sample analysis time is only 1 min, detection limit can reach to 10 ppb. Key words:deoxynivalenol, MALDI-TOF MS
Hsieh, Cheng-Chuan, and 謝政娟. "High-Throughput Chip-Based Solid Phase Extraction Coupling with Inductively Coupled Plasma-Mass Spectrometry for Online Determination of Trace Elements." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/30122884080524470557.
Повний текст джерела國立清華大學
生醫工程與環境科學系
99
Although inductively coupled plasma-mass spectrometry (ICP-MS) is a very powerful technique for trace metal analysis, the sample concomitants often lead to significant spectral interference and/or loss of sensitivity during the determination process. Therefore, incorporating an efficient online pretreatment technique to ICP-MS is considered as an indispensable alternative to concentrate analyte ions and to minimize the adverse effects caused by the concomitant matrices. Among various sample pretreatment techniques, solid-phase extraction (SPE) method is generally superior in terms of their simplicity and efficacy. Over the past decade, several chip-based SPE techniques have been extensively applied to analytical works. Its unique characteristics of high surface-to-volume ratio and short molecular diffusion distance enable a new pretreatment paradigm that has the potential to effectively separate analyte species from sample matrix. Even so, the realization of high-throughput analyses for chip-based SPE techniques remains difficult and rare until now. In general, increasing the operation flow rate is straightforward for achieving high-throughput SPE procedures. However, microdevices used for high-throughput analyses are often limited to large increase in the hydrodynamic resistance with increasing operation flow rate. Also, complete mixing of two fluids (sample and buffer solutions) within a reasonable time is quite difficult due to the low Reynolds number on the micro scale. To overcome the limitations, in this study, a polytetrafluoroethylene (PTFE) bead was implanted into the mixing chamber to change the streamline of solutions for efficient mixing and then the chip-based SPE device which composed of circularly multichannel pattern was employed to carry out the SPE procedures. Based on our results, this hyphenated system could effectively eliminate the salt-interference resulted from the salt matrices and preconcentrate desired elements even the extraction flow rate was extremely high. In addition, dilute samples were further used to demonstrate the first successfully high-throughput on-chip SPE of trace metal ions from high-salt content samples.