Дисертації з теми "Hena"
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1
Lorek, Emilia. "”Är han en sån där Hen eller?” - En studie om begreppet Hens möjliga funktion i skolans jämställdhetsarbete." Thesis, Malmö högskola, Lärarutbildningen (LUT), 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-34874.
Повний текст джерелаАнотація:
Uppsatsens syfte var att undersöka om begreppet Hen kan fylla en funktion i skolans jämställdhetsarbete. Jag har genomfört både kvalitativa och kvantitativa studier genom tre djupdykningar, en enkätundersökning som verksamma lärare svarade på, tre semistrukturerade intervjuer med informanter som främst representerar ett akademiskt perspektiv samt en textanalys från ett kapitel av en lärobok. Resultatet har jag teoretiserat genom Hirdmans begrepp det stereotypa genuskontraktet, Butlers teori om performativitet och genom den binära genusuppdelningens betydelse i genusordningen samt ställt resultatet i relation till tidigare forskning. Resultatet av teoretiseringen pekar i stor utsträckning på att de verksamma lärarna ser ett problem med det stereotypa genuskontraktet samt performativitet, men inte den binära genusuppdelningen. Informanterna såg problem med alla dessa begrepp då de är en del av genusordningen. I textanalysen framkom att läromedlet inte stämde överens med skolans uppdrag, att aktivt motarbeta traditionella könsmönster, då texten agerade konserverande i samtliga begrepp. Slutsatsen av uppsatsen är att eftersom genusordningen upprätthålls i skolan genom den binära genusordningen och performativa processer, har Hen en tydlig teoretisk funktion i skolan jämställdhetsarbete och kan därför vara gynnsamt att införa i skolans verksamhet.
2
Ramos, Alexandre Floriani. "Efeito de progesterona e benzoato de estradiol na dinâmica folicular e produção in vitro de embriões bovinos." Universidade Federal de Minas Gerais, 2006. http://hdl.handle.net/1843/HESA-6ZQQG8.
Повний текст джерелаАнотація:
The purpose of this study was to evaluate the effect of estradiol benzoate (EB) and progesterone/norgestomet on follicular dynamics of heifers aspirated every 7 d through synchronization of the follicular wave emergence in order to increase the number of follicles and quality of the recovered oocytes. Three experiments were designed in which crossbred Simmental x Nelore heifers were randomly divided into three groups, with all animals participating in all groups. In experiments 1 and 2, heifers were synchronized using a progestin implant for 7 d followed by a 150 µg im injection of d-Cloprostenol and aspiration of all ovarian follicles greater than 3 mm in diameter. In experiment 1 (n = 12), follicles from group 2X were aspirated twice a week with 4- and 3-day interval, and follicles from 1X and 1X-EB heifers were aspirated once a week. Heifers from group 1X-EB also received an im injection of 2 mg of EB immediately after OPU. In experiment 2 (n= 12), all heifers were aspirated once a week. Heifers from 0EB group did not receive EB, and from 2EB and 5EB received 2 mg and 5 mg of EB immediately after OPU. In experiment 3 (n = 15), heifers were synchronized with 2 mg EB and progesterone intravaginal implant (DIB) for seven d followed by an im PGF2_ injection. On day 4 of the subsequent estrous cycle, heifers received a DIB and had all ovarian follicles larger than 3 mm in diameter aspirated, followed by a PGF2_ injection on day seven. Heifers in groups 0h-EB (n = 4), -12h-EB (n = 6) and -24h-EB (n = 5) received 2 mg of EB immediately after OPU and twelve and twenty four h before, respectively. The semen, media used and culture conditions were equal for all groups. Ultrasound evaluations of number of follicles greater than 3 mm in diameter and size of the largest follicle at the time of OPU were performed. All recovered oocytes were in vitro matured and fertilized (D0). Cleavage rate on D2 and blastocyst production on D7 was evaluated. During a 7-day period, daily measurement of the size of the greatest follicle was performed by ultrasonography, and daily blood collection was done for evaluation of serum FSH concentrations. Although the use of EB in association with a norgestomet/progesterone implant seven d prior to OPU have altered follicular wave profile and FSH concentration, it was not able to efficiently synchronize the emergence of follicular wave and follicular deviation. Moreover, it was not enough to improve number and quality of the recovered oocytes. This study failed to demonstrate a positive effect of OPU earlier after wave emergence, when the effect of dominance should be less pronounced, on IVP in bovine. Moreover, the use of EB in association with a progesterone implant 12 and 24 h prior to OPU compromised the in vitro embryo production in a program of OPU with a 7 d interval between sessions.O objetivo deste estudo foi avaliar o efeito de benzoato de estradiol (BE) e progesterona/norgestomet sobre a dinâmica folicular de novilhas aspiradas a cada sete dias, sincronizando a emergência da onda folicular para aumentar o número de folículos e qualidade dos ovócitos. Em três experimentos, novilhas meio sangue Simental x Nelore foram divididas aleatoriamente em três tratamentos, com todos os animais participando de todos os tratamentos. Nos experimentos 1 e 2, as novilhas foram sincronizadas com implante de progesterona/progestágeno por 7 d seguido da administração im de 150 µg de d-Cloprostenol e aspiração de todos os folículos maiores que 3 mm de diâmetro. No Experimento 1 (n=12), os folículos das novilhas do tratamento 2X foram aspirados duas vezes por semana, com intervalo de 3 e 4 d, e os das novilhas do tratamento 1X e 1X-BE uma vez por semana. As novilhas do tratamento 1X-BE receberam 2 mg de BE im após a aspiração. No Experimento 2 (n = 12), as novilhas do tratamento NBE não receberam BE, e as dos tratamentos 2BE e 5BE receberam 2 mg e 5 mg após a aspiração. No Experimento 3 (n = 15), as novilhas foram sincronizadas com 2 mg de BE e implante intravaginal de progesterona (DIB) por sete dias, seguido de administração de PGF2_. No dia quatro do ciclo estral subseqüente, as novilhas receberam DIB e tiveram todos os folículos maiores que 3 mm de diâmetro aspirados, seguido da administração de PGF2_ no dia sete. As novilhas do tratamento 0h-BE (n= 4), -12h-BE (n = 6) e -24h-BE (n = 5) receberam 2 mg de BE imediatamente após a aspiração, doze e vinte e quatro horas antes, respectivamente. O sêmen, meios utilizados e as condições de cultivo foram iguais para todos os tratamentos. O número de folículos maior que 3 mm e o tamanho do maior folículo foram avaliados por ultra-sonografia no momento da aspiração folicular. Todos os ovócitos aspirados foram maturados e fecundados in vitro (D0), com a taxa de clivagem e a taxa de blastocisto avaliadas no D2 e D7, respectivamente. Durante uma semana foi realizada, diariamente, a mensuração do tamanho do maior folículo, por ultra-sonografia, e coletado sangue para a quantificação da concentração de FSH. Embora o uso de 2 mg de BE associado a progestágeno/progesterona tenha alterado o padrão de crescimento folicular e a concentração de FSH, não foi capaz de sincronizar eficientemente a emergência da onda e o desvio folicular, além de não aumentar o número e a qualidade dos ovócitos recuperados. Este estudo falhou em mostrar o efeito positivo da aspiração folicular logo após a emergência da onda folicular, quando o efeito da dominância seria menos evidente, sobre a produção in vitro de embriões bovinos. Além disso, o uso de BE associado a um dispositivo de progesterona, 12 h ou 24 h antes da aspiração folicular, prejudicou a produção de embriões in vitro, em um programa de aspiração com intervalo de sete dias entre sessões.
3
Moraes, Douglas Teixeira. "Efeitos dos programas de luz sobre o desempenho, rendimentos de abate, aspectos econômicos e resposta imunologica em frangos de corte." Universidade Federal de Minas Gerais, 2006. http://hdl.handle.net/1843/HESA-6ZQP72.
Повний текст джерелаАнотація:
To evaluate the effect of different lighting programs on performance, feed intake at each six hours, economic aspects, carcass characteristics and antibodies production to Newcastle disease of broiler chickens an experiment was conducted with 840 young males chicks of the Cobb-500 lineage. The treatments defined according to the photoperiod were: 23L 23 hours of light and 1 hour of darkness from 1 to 45 days old; CRES 23 hours of light from 1 to 7 days old, without artificial light during the 8
and 21 days old, 14 hours of light from 22 to 28 days old, 18 hours of light from 29 to 35 days old and 23 hours of light from 35 to the 45 days old; 16L 16 hours of light from 1 to 45 days old and the NAT program, without artificial light during all the raising period. The poultry was located in an experimental poultry house, 11,60 birds for m2. The lighting intensity at the birds level was of 20 lux during all raising period that was from 1 to 45 days of age. The rations used were the same and offered at pleasure. The statistical design was the complete randomized divided in four treatments with six repetitions of 35 birds each. Analysis of variance with mean separation was made using SNK´s multiple range test for
performance, carcass characteristics, economic aspects and immunological response. Broiler chickens of CRES lighting program presented higher body weight (p<0,05) than birds of the others lighting programs that were similar between then (p>0,05). The feed intake of the birds of the CRES lighting program was higher than the birds of the 23L and NAT (p<0,05) and similar to the 16L treatment (p>0,05). Feed
conversion was not observed differences between treatments (p>0,05). The livability was higher in birds of the NAT lighting program than the 23L lighting program (p<0,05) and similar to the others treatments (p>0,05). Feed intake during 24 hours period was not observed differences between treatments at 27 days of age (p>0,05). Feed intake during 24 hours period at 34 days of age was higher in the 16L treatment than the others treatments (p<0,05) that were similar between then (p>0,05). The carcass, foot, head, abdominal fat, liver, heart and gut yield were not observed differences between treatments (p>0,05). The birds of the 23L lighting program presented breast muscle higher than the others treatments (p<0,05). The cost of the broiler kilogram was not observed differences between treatments (p>0,05). The antibody levels against Newcastle disease were not observed differences between treatments (p>0,05).Para avaliar os efeitos dos programas de luz sobre o desempenho, consumo de ração a cada seis horas aos 27 e 34 dias de idade, aspectos econômicos, rendimentos de abate e resposta à vacinação contra Newcastle foram utilizados 840 pintos machos da linhagem Cobb-500. Os tratamentos, definidos pelos períodos de luminosidade diária foram os seguintes: 23L quase contínua 23 horas de luz e uma hora de escuro do primeiro aos 45 dias de idade das aves; CRES 23 horas de luz até os 7 dias de idade, luz natural dos 8 aos 21 dias de idade, 14 horas de luz dos 22 aos 28 dias de idade, 18 horas de luz dos 29 aos 35 dias e 23 horas de luz dos 36 dias ao abate; 16L 16 horas de luz e 8 horas de escuro do primeiro aos 45 dias de idade e o programa NAT sem suplementação de luz artificial durante todo o período de criação. As aves foram alojadas em galpão experimental sendo a densidade de 11,60 aves/m2. O nível de iluminamento na altura das aves foi de 20 lux durante todo o período de criação que foi de 1 a 45 dias de idade. As rações utilizadas foram as mesmas para todas as aves e oferecidas à vontade. O delineamento experimental utilizado foi inteiramente ao acaso, com 4 tratamentos, 6 repetições de 35 aves. As diferenças entre as médias foram comparadas pelo teste de Student-Newman-Keuls (SNK) para desempenho, consumo, aspectos econômicos, rendimentos de abate e sorologia para detecção de anticorpos contra a doença de Newcastle. As aves submetidas ao programa de luz CRES apresentaram peso vivo superior quando comparado aos demais tratamentos (p<0,05), os quais apresentaram resultados semelhantes aos 45 dias de idade (p>0,05). O consumo de ração também foi superior nas aves do tratamento CRES quando comparado aos tratamentos 23L e NAT (p<0,05), e semelhante ao tratamento 16L (p>0,05). A conversão alimentar não foi influenciada pelos tratamentos (p>0,05). A viabilidade das aves do tratamento NAT foi superior em relação às aves do tratamento 23L (p<0,05) e semelhante aos demais tratamentos (p>0,05) aos 45 dias de idade. O consumo no período de 24 horas não foi influenciado pelos tratamentos aos 27 dias de idade (p>0,05). Aos 34 dias de idade, as aves do tratamento 16L apresentaram consumo superior às aves dos demais tratamentos (p<0,05), as quais apresentaram consumos semelhantes entre si (p>0,05). Os rendimentos de carcaça, pés, cabeça+pescoço, gordura abdominal, dorso, fígado, coração e intestinos não foram influenciados pelos tratamentos (p>0,05). As aves do tratamento 23L apresentaram rendimento de peito superior aos demais tratamentos (p<0,05). O custo do kg de frango não foi influenciado pelos tratamentos (p>0,05). Os títulos geométricos médios de anticorpos contra a doença de Newcastle não foram influenciados pelos tratamentos (p>0,05).
4
Cunha, Arildo Pinto da. "Controle estratégico de Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae) e Anocentor nitens (Neumann, 1897) (Acari ixodidae) em eqüinos, Minas Gerais, Brasil." Universidade Federal de Minas Gerais, 2006. http://hdl.handle.net/1843/HESA-6ZWP3H.
Повний текст джерелаАнотація:
The aim of this study was to evaluate the application of a strategic control program of Amblyomma cajennense in equines, in the city of Palma, MG. Before the implantation of the program the parasitic loads of the A. cajennense and Anocentor nitens were observed, monthly, in the period from October of 2003 to March of 2004, in the same period of the following year the comparative analyses were carried. The acaricide treatments were carried to each seven days and divided in two batteries: the first one beginning in April of 2004, composed of eight treatments and second beginning in July of 2004, composed of five treatments, respectively, aiming to reach the larvae and nymphs of the A. cajennense. The chemical base used was pyrethroid - cypermethrin 0.015%, to bathe the equines. Clinical and hematological aspects (hemogram and serum biochemical) of the equines were analyzed before the control program. Also the effect of the program destined to the control of the A. cajennense on the population of A. nitens was observed. After the control program had a reduction of 44.85% of adults A. cajennense of October of 2004 to March 2005, and 59.74%, in February and March of 2005. The results of the clinical and hematological examinations of the equines had not evidenced alterations undesirable, but the improvement in some hemogram parameters. However the acaricide treatments had not determined significant reduction of the parasitic load of A. nitens in the equines. The control program revealed effective and safe for the control of the A. cajennense in the equines. However, it was not possible to carry through all the stages of the initial planning, once the study was carried on a commercial property, searched to define some critical points for the implantation of this type of technology in field situations. The interference was evidenced of the management model and structure of the agricultural company with the operational and technical aspects of the proposal of control of the tick, factors that can be determinant in the degree of success of the sanitary control. The permanence of the levels of infestation for A. nitens can be by no application of acaricide in nasal diverticulums of the equines, since this is an important site of parasitism of A. nitens in equines, however theres no compatible methods or acaricide formulations with the particularities of this anatomical structure.Avaliou-se a aplicação de um programa de controle estratégico de Amblyomma cajennense em eqüinos, no município de Palma, Minas Gerais. Antes da implantação do programa foram observadas as cargas parasitárias de A. cajennense e Anocentor nitens, mensalmente, no período de outubro de 2003 a março de 2004 e no mesmo período do ano seguinte foram realizadas as observações comparativas. Os tratamentos carrapaticidas foram realizados a cada sete dias e divididos em dois módulos: o primeiro com início em abril de 2004, composto de oito banhos e o segundo com início em julho de 2004, composto de cinco banhos, respectivamente, visando atingir as larvas e ninfas de A. cajennense. Empregou-se a base química piretróide - cipermetrina 0,015%, para banhar os eqüinos. Foram analisados aspectos clínicos e hematológicos (hemograma e bioquímica sérica) dos eqüinos perante o programa de controle. Também foram observados os efeitos do programa destinado ao controle do A. cajennense sobre a população de A. nitens. Após o programa de controle houve uma redução de 44,85% de adultos de A. cajennense no período de outubro de 2004 a março 2005, e de 59,74%, em fevereiro e março de 2005 em relação ao período anterior. Os resultados dos exames clínicos e hematológicos dos eqüinos não evidenciaram alterações. Observou-se a melhora em alguns parâmetros do hemograma em função das condições de saúde dos animais após o controle do carrapato. Porém os banhos carrapaticidas não determinaram redução significativa da carga parasitária de A. nitens nos eqüinos. O programa de controle mostrou-se efetivo para o controle do A. cajennense e seguro para os animais. No entanto, não foi possível realizar todas as etapas do planejamento inicial, uma vez que, o estudo foi realizado em uma propriedade comercial, contudo, buscou-se definir alguns pontos críticos para a implementação deste tipo de tecnologia em situações de campo. Puderam-se evidenciar a interferência do modelo de gestão e estrutura da empresa rural com os aspectos técnicos e operacionais da proposta de controle do carrapato, fatores que podem ser determinantes no grau de sucesso do controle sanitário. A permanência dos níveis de infestação por A. nitens, pode ser devida, a não aplicação do produto carrapaticida no divertículo nasal dos animais, pois este é um importante sítio de parasitismo de A. nitens em eqüinos, visto que não existem métodos ou formulações carrapaticidas compatíveis, técnica e operacionalmente, com as particularidades desta estrutura anatômica.
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Silva, Carla Maria Osorio. "Efeito da prednisona em lesão medular aguda experimental em ratos (Rattus novergicus)." Universidade Federal de Minas Gerais, 2006. http://hdl.handle.net/1843/HESA-6WPMA2.
Повний текст джерелаАнотація:
We develop a model to evaluate the effects of prednisone when applied at different time intervals after moderate and acute spinal cord injury. A pilot study using 10 Rattus novergicus allowed us to define a spinal cord injury protocol as 50.5g applied during five minutes with estereotoxic equipment. Later 25 rats were submitted to this protocol and randomly divided into five treatment groups: one group received placebo 8 hours after injury and the remaining four groups received prednisone eight, 24, 48 and 120 hours after injury. Prednisone was applied at 2 mg/Kg dosage during five days and progressively reduced until the 26th day. Animals were evaluated by motor capacity, proprioceptive positioning, searching ness reflex, inclined plan and sensibility to pain. Each rat was sacrificed 33 days after surgery for histopathology of the spinal cords that revealed degeneration of the rootlets, spinal cord necrosis and reactive gliosis, with no significant differences among treatment groups. Results for the various tests did not seem to be consistent and hence we could not conclude which treatment group was the best. Late administration of high dosages of prednisone to animals with acute spinal cord injury had no deleterious effects on neurological recoveryObjetivou-se desenvolver um modelo de trauma medular agudo e moderado, para avaliar o efeito da prednisona em diferentes tempos após esse trauma. Primeiramente foram utilizados 10 Rattus novergicus submetidos à compressão medular empregando-se um aparelho estereotáxico com diferentes pesos e tempos e padronizou-se a utilização de 50,5g por cinco minutos. Posteriormente, utilizou-se 25 ratos submetidos ao trauma proposto e divididos em cinco grupos de cinco. O grupo A recebeu placebo oito horas após o trauma; os grupos B, C, D e E receberam prednisona oito, 24, 48 e 120 horas após o trauma, respectivamente. A prednisona foi administrada na dose de 2mg/Kg, durante cinco dias com diminuição progressiva até o 26º dia. Os animais foram avaliados conforme a capacidade motora; posicionamento proprioceptivo; reflexo de localização; plano inclinado e sensibilidade dolorosa. Após 33 dias da cirurgia os animais foram sacrificados e, realizou-se a histopatologia das medulas espinhais onde constatou-se degeneração das raízes nervosas, necrose medular e reação glial, sem diferenças entre os grupos. Verificou-se falta de correlação entre os testes indicando que não houve um grupo superior. A utilização da prednisona na lesão medular não teve efeito diferenciado com o tempo e a administração tardia não foi prejudicial à recuperação neurológica.
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Borges, Barbara Kellen Antunes. "Fatores de risco para leishmaniose visceral em Belo Horizonte, Minas Gerais, 2006." Universidade Federal de Minas Gerais, 2006. http://hdl.handle.net/1843/HESA-6ZWQA5.
Повний текст джерелаАнотація:
The present study was conducted in order to analyze the possible risk factors involved the occurrence of Visceral Leishmaniasis (VL) in Belo Horizonte/MG, in 2006. It was made a case-Control study using questionnaire, images registration inside and outside of the affected dwellings and collection of canine blood. The sample was constituted by 82 human cases of VL occurred in 2004 and 164 controls (neighbors of the left and of the right of the cases). The nine regional administrative branch of the Town Hall of Belo Horizonte participated with residences in the study, in spite of not have aimed at geographical level of the disease. The collection of data of the cases happened with the relatives' participation, when it was not possible to do with the own cases. The biological material sample was sent to the laboratory of Leishmaniasis of the Veterinary School of UFMG for accomplishment of laboratory analysis (ELISA and IFAT). The interviewed population was characterized for being adult, with feminine sex slightly predominant. Larger occurrence of VL was noticed among children with age less or equal to 10 years. In relation to the dwellings, the risk factors show significant effect were: if there is a hen house in the propriety, humidity, garbage, fallow lands, If there is fruit or vegetable crop in the house, besides the presence of others animals such as rodents, birds and cats. Due to the low index of canine seropositivity, the dog presence in the residence was considered without statistical effect, which can be inferred a effective control of the animals soropositive for VL in areas with occurrence of human cases. The orientation of the people about the main VL information showed a potential protection, being estimated 2,24 times less likely of have a diseased among the people that have any knowledge about VL.Objetivou-se analisar os fatores de risco envolvidos na ocorrência de Leishmaniose Visceral (LV) em Belo Horizonte/MG, em 2006. Para tal, fez-se estudo de caso-controle por meio de entrevistas domiciliares, questionário semi-estrurado, registro de imagens do intra e peridomicílio e coleta de sangue canino. A amostra foi constituída por 82 casos humanos de LV ocorridos em 2004 e 164 controles (vizinhos da esquerda e da direita dos casos). As nove regionais administrativas de Belo Horizonte tiveram residências participantes no estudo, apesar desse não ter objetivado mapeamento geográfico da doença. A coleta de dados dos casos ocorreu com a participação de familiares, quando não foi possível fazê-la com os próprios acometidos. O material biológico coletado foi encaminhado para o laboratório de Leishmaniose da Escola de Veterinária da UFMG para realização de análises sorológicas (Ensaio Enzimático - ELISA e Reação de Imunofluorescência Indireta - RIFI). A população entrevistada se caracterizou por ser adulta, com discreto predomínio do sexo feminino. Notou-se maior acometimento da LV em crianças com idade inferior ou igual a 10 anos. Em relação às residências, os fatores de risco que se mostraram significativos foram: a existência de galinheiro, umidade, lixo, terrenos baldios, cultivo de plantas frutíferas e arborização ao redor do domicílio, além da presença de outros animais, tanto domésticos quanto silvestres, como roedores, pássaros e gatos. Devido ao baixo índice de soropositividade canina, a presença de cão na residência foi considerada sem significância estatística, o que sinaliza um controle efetivo dos animais soropositivos para LV em áreas com ocorrência de casos humanos da doença. A orientação das pessoas sobre as principais informações que abordam a doença mostrou potencial de proteção, sendo estimado um decréscimo no risco de ser acometido de 2,24 vezes para pessoas que detenham quaisquer conhecimentos sobre a LV.
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Goulart, Carlos Eduardo Silveira. "Valores hematológicos de referência para papagaios- verdadeiros (Amazona aestiva - Psittacidae) mantidos em cativeiro." Universidade Federal de Minas Gerais, 2006. http://hdl.handle.net/1843/HESA-6ZWPQV.
Повний текст джерелаАнотація:
The present work aimed to describing values and aspects that can serve as parameters of physiological reference for the hematology of the blue-fronted-parrot (Amazona aestiva - Psittacidae), as well as determining the occurrence of hemoparasites, in an established commercial breeder in Betim - Minas Gerais, Brazil. Fifty eight blue-fronted-parrot (Amazona aestiva) on captivity for at least three years, adults, of both sex and in adequate conditions of health were used. After an statistics analysis of the results, the following basic parameters were found (Mean + Standard Derivation): Erythrocytes (x106/mm3): 2,54 + 0,45; Hemoglobin (g/dL): 16,28 + 1,13; Hematocrit (%): 44,66 + 4,45; Mean corpuscular volume (µ3ou fL): 179,99 + 32,15; Mean corpuscular hemoglobin (µµg ou pg): 65,50 + 8,15; Mean corpuscular hemoglobin concentration (g/dL or %): 36.51 + 2,90; immature erythrocytes (x103/mm3): 32,06 + 1,85; Thrombocytes (x 103/mm3): 16,61 + 7,45; Leukocytes (x 103/mm3): 7,01 + 4,15; Heterphils (x 103/mm3): 3,40 + 2,05; Lymphocytes (x 103/mm3): 3,37 + 2,83; Monocytes (x 103/mm3): 0,16 + 0,16; Basophils (x 103/mm3): 0,08 + 0,09; Eosinophils (x103/mm3): 0,02 + 0,05; immature granulocytes (x /mm3): 37,95 + 84,17. Parameters for subpopulations of immature erythrocytes and immature granulocytes in the peripheral blood, as well as morphologic characteristics of the subpopulations of leukocytes and thrombocytes were also evaluated. The occurrence of Plasmodium sp. in peripheral blood occurred in 29,31% of birds, and the parasitemy was 0,031% + 0,031%. Significant differences had not been observed between: sex; clinical and reproductive parameters. There was no statistical difference on hematological parameters between both groups (with or without Plasmodium sp.), except for the parameters of counting of juvenile polychromatic erythrocytes and advanced polychromatic erythrocytes (P = 0,004 and 0,025 respectively).O presente trabalho objetivou descrever valores e aspectos que possam servir como parâmetros de referência fisiológica para a hematologia do papagaio-verdadeiro (Amazona aestiva - Psittacidae), bem como determinar a ocorrência de hemoparasitoses em um criatório comercial estabelecido em Betim Minas Gerais, Brasil. Para tanto utilizaram-se 58 (cinqüenta e oito) papagaios-verdadeiros (A. aestiva) cativos a pelo menos três anos, adultos, de ambos os sexos e em condições adequadas de saúde. Após analises estatísticas dos dados, foram obtidos os seguintes parâmetros fundamentais (Média + Desvio Padrão): Hemácias (x 106/mm3): 2,54 + 0,45; Hemoglobina (g/dL): 16,28 + 1,13; Hematócrito (%): 44,66 + 4,45; Volume corpuscular médio (µm3 ou fL): 179,99 + 32,15; Hemoglobina corpuscular média (µµg ou pg): 65,50 + 8,15; Concentração hemoglobina corpuscular média (g/dL ou %): 36,51 + 2,90; Eritrócitos imaturos (x 103/mm3): 32,06 + 1,85; Trombócitos (x 103/mm3): 16,61 + 7,45; Leucócitos (x103/mm3): 7,01 + 4,15; Heterófilos (x 103/mm3): 3,40 + 2,05; Linfócitos (x 103/mm3): 3,37 + 2,83; Monócitos (x 103/mm3): 0,16 + 0,16; Basófilos (x 103/mm3): 0,08 + 0,09; Eosinófilos (x 103/mm3): 0,02 + 0,05; Granuloblastos (x /mm3): 37,95 + 84,17. Também foram avaliados parâmetros referenciais para subpopulações de eritroblastos e granuloblastos no sangue periférico, bem como características morfológicas das subpopulações de leucócitos e trombócitos. Neste estudo, observou-se a ocorrência de 29,31% de aves hemoparasitadas por Plasmodium sp. com parasitemia de 0,031% + 0,031%. Não foram observadas diferenças significantes entre: sexos; parâmetros clínicos e reprodutivos. Quanto ao hemograma, não se evidenciou diferença relevante nos parâmetros hematológicos entre sexos, exceto para os parâmetros de contagem dos eritrócitos policromáticos jovens e eritrócitos policromáticos avançados (P = 0,004 e 0,025 respectivamente).
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Aronsson, Emma. ""Och vad Gud beträffar så tror jag att hen går hel ur det här." : En studie av ordet hens funktion i dagspress." Thesis, Umeå universitet, Institutionen för språkstudier, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-73220.
Повний текст джерелаАнотація:
I denna studie analyseras funktionen av ordet hen i syfte att undersöka hur ordet används i dag. Hen fungerar som könsneutralt pronomen i svenskan men fyller även andra syntaktiska och könsöverskridande funktioner. Materialet har analyserats med en kvantitativ ansats och består av texter från dagspress där de olika förekomsterna av hen har kategoriserats efter funktion och texttyp. Uppsatsens avsnitt om teori behandlar språkplanering, feministisk språkplanering och hens uppkomst. Resultatet visar att hen främst fyller syntaktiska funktioner i dagspress, där 143 av 167 förekomster av ordet användes i funktioner som i den här studien kategoriserats som syntaktiska. Den vanligaste funktionen i materialet var hen i generisk referens. Ordet användes inom 15 olika texttyper inom dagspress, främst artiklar och krönikor, där hen i generisk referens var vanligast i artiklar och hen i maskerande funktion var vanligast i krönikor.This study analyzes the function of the Swedish gender-neutral pronoun hen with the purpose of examining how the word is used today. Aside from being used as a gender-neutral pronoun, hen is also used in other syntactical functions as well as gender-transcending functions. The study material consists of texts from daily press that have been analyzed through a quantitative method. The various uses of hen have been categorized by function and text type. The theoretical part of the study introduces language planning, feminist language planning, and the origins of hen. The results of the study indicate that hen mainly fills a syntactical function in daily press, as 143 out of 167 uses of the word were in functions that can be categorized as syntactical. The most common function of hen found in the material was hen in generic reference. Hen was used in 15 different text types within daily press, most commonly in articles and columns. In articles, hen in generic reference was most common, while in columns most of the found hen were used in a concealing function.
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Blomberg, Sara. "Hela staden ska vara med! : Hela staden ska vara med!" Thesis, Örebro universitet, Institutionen för humaniora, utbildnings- och samhällsvetenskap, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-37078.
Повний текст джерела
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Norstedt, Karin. "Hela barnet hela dagen : Om fritidshemspersonalens tillgång till specialpedagogiskt stöd." Thesis, Malmö universitet, Malmö högskola, Institutionen för skolutveckling och ledarskap (SOL), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-40084.
Повний текст джерелаАнотація:
Norstedt, Karin (2020). Hela barnet hela dagen, Om fritidshemspersonalens tillgång till specialpedagogiskt stöd. Specialpedagogprogrammet, Institutionen för skolutveckling och ledarskap, Lärande och samhälle, Malmö universitet, 90 hp. Förväntat kunskapsbidrag Det här arbetet väntades kunna bidra till att det forskas mer kring specialpedagogik på fritidshemmet. Att belysa i vilken omfattning en del personal i fritidshemmet upplevde stöd, ämnade studien ge en inblick i ämnet. Det här arbetet kan bidra med insikt och kunskap kring vikten av att det specialpedagogiska stödet till lärare ska fördelas efter behov även för personal på fritidshemmen. För specialpedagogen kan arbetet bidra till att belysa vikten av att fritidshemmet inkluderas i arbetet för barn i behov av särskilt stöd Syfte och frågeställningar Syftet med undersökningen är att ta reda på i vilken utsträckning personal i fritidshemmen har tillgång till specialpedagogiskt stöd samt hur detta stöd ser ut utifrån givna kategorier. Frågeställning Hur upplever verksamma inom fritidshemmet omfattning och innehåll i specialpedagogiskt stöd Vilket stöd ges och vilket stöd efterfrågas? Teori Den teoretiska utgångspunkten var Bronfenbrenners (1979) miljöekologiska systemteori som menar att delarna inte kan verka var för sig utan måste ses ur ett helhetsperspektiv. I sammanhanget är fritidshemmet en del av barnets hela skoltid dvs. helheten. Att bearbeta och analysera utifrån denna teori fyllde sitt syfte väl gällande att se delarna som en del av helheten. Teorin bearbetade materialet utifrån och in, där Bronfenbrenners (1979) ekologiska systemteori startar med makronivån som innefattade skollag och politik, värderingar och normer. Sedan följer exonivå som bland annat innefattar skolans organisation, sjukvård och andra organisationer som kan påverka individen indirekt. Mesonivån användes som ett sätt att se på samverkan mellan skola och hem och dess kunskap om varandra. Mikronivån är individen och den miljö och relationer den möter under dagen i skolans lokaler, dvs fritids-skola-fritids. Metod För att ta reda på hur många anställda (lärare i fritidshem, fritidspedagog och annan utbildning) i fritidshemmet som upplevde ett specialpedagogiskt stöd var kvantitativ enkätundersökning den metod som lämpade sig bäst. I enkäten användes slutna frågor för att kunna besvara frågeställningen. Detta sammanställdes i olika tabeller och figurer för att få en uppfattning kring stödet. Ett fritextsvar fanns under en del frågor och som resulterade i en djupare inblick i hur respondenterna upplevde stödet eller icke stödet. Resultat Resultatet visade att det finns en signifikant skillnad i upplevelsen av specialpedagogiskt stöd. Lite mer än 2/3 upplevde ett stöd som inte kan ses som tillräckligt (Ja, men inte i den utsträckning jag önskar eller Nej). Resultatet visade att det stödet som efterfrågas främst är handledning och stöttning kring lärmiljön. Detta gav indikationer på att det måste ske förändringar i systemet som skapar förutsättningar för specialpedagoger och verksamma i fritidshemmet att utveckla och vidmakthålla en verksamhet som lever upp till skollagen Specialpedagogiska implikationer Specialpedagogens uppgift är att se barnet som en helhet, dvs allt som på något sätt berör barnet, och det är helheten som ska ligga till grund för det stöd som ges. Det betyder att barnets kunskapsutveckling, sociala utveckling och hälsa är delar av helheten. Dessa delar existerar från tidig morgonfritids till lektionstid och till eftermiddagsfritids. Genom att handleda och använda fritidshemmens kunskap kring aspekter som direkt eller indirekt kan påverka elevens helhet, kan specialpedagogen vara med och förändra, stötta och anpassa även fritidshemmets verksamhet. Specialpedagogen måste ges förutsättningen till samverkan med alla delar och dessa förutsättningar är en del av huvudmännens uppdrag. Specialpedagogiken och det stöd specialpedagogen kan ge, bör innefatta fritidshemmet.
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Ytterberg, Adam. "Partikel hela vägen." Thesis, Konstfack, Institutionen för Konst (K), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:konstfack:diva-6074.
Повний текст джерелаАнотація:
i Uppsatsen försöker jag beskriva min senaste tid inom konstinstitutionen där jag brottats med vad det är jag vill påstå att min konst handlar om. jag demonstrerar med sakligt argumenterande och med en poetisk ton mitt sökande efter nyanser på min palett av skapelseprocesser för att komma närmare helhetsbilder.
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Wolf, Thomas. "Die Systemsoftware für den First Level Trigger des HERA-B Experiments." [S.l.] : [s.n.], 1998. http://deposit.ddb.de/cgi-bin/dokserv?idn=957878028.
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Hebbel, Kirstin. "Measurement of charm production in deep inelastic ep scattering at HERA." [S.l. : s.n.], 2000. http://www.sub.uni-hamburg.de/disse/198/thesis.pdf.
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Desler, Kai. "Elastic and proton dissociative r0 [rho 0] photoproduction at HERA." [S.l. : s.n.], 2000. http://www.sub.uni-hamburg.de/disse/458/diss.pdf.
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Karstens, Falk. "Charm-Produktion in Tiefinelastischer Streuung am ZEUS-Experiment in der Datennahmeperiode von HERA-II /." Freiburg, Br. : Rombach, 2007. http://deposit.d-nb.de/cgi-bin/dokserv?id=2980892&prov=M&dok_var=1&dok_ext=htm.
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Loizides, John Harry. "Charm at HERA I and HERA II with the ZEUS experiment." Thesis, University College London (University of London), 2005. http://discovery.ucl.ac.uk/1444946/.
Повний текст джерелаАнотація:
Inclusive jet cross sections in photoproduction for events containing a D* meson have been measured with the ZEUS detector at HERA using an integrated luminosity of 78.6 pb_1. The events were required to have a virtuality of the incoming photon, Q2, of less than 1 GeV2, and a photon-proton centre-of-mass energy in the range 130 < Wlp < 280 GeV. The measurements are compared with next-to-leading-order (NLO) QCD calculations. Good agreement is found with the NLO calculations over most of the measured kinematic region. Requiring a second jet in the event allowed a more detailed comparison with QCD calculations. The measured dijet cross sections are also compared to Monte Carlo (MC) predictions which incorporate leading-order matrix elements, followed by parton showers and hadronisation. The NLO QCD predictions are in general agreement with the data although differences have been isolated to regions where extra parton radiation is present. The MC models give a better description of the shape of the measured cross sections. This thesis ends with a look at the first HERA II data. The decay of D mesons were studied and for the first time the newly installed micro vertex detector (MVD) was used to tag charm mesons using secondary vertices arising from the decay of (c * D or b * c * D). This shows that the MVD will be a powerful tool in the future analyses of heavy quark production at ZEUS.
17
Gärling, Robert. "Walter Henn und Dresden." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-24424.
Повний текст джерелаАнотація:
Jeden Studierenden prägt seine Alma mater ein Leben lang. Walter Henns Laufbahn begann an der Technischen Hochschule Dresden, über die er sagte: „Der eine oder der andere wirft mir vielleicht vor, dass ich undankbar bin, wenn ich diese Hochschule verlasse, aber an der Hochschule, an der man studiert hat, promoviert hat, Assistent und schließlich Professor war, an dieser Hochschule wächst man nicht mehr weiter.“ (Manuskript-Fragment der Abschiedsvorlesung, S. 36, Mscr.Dresd.App. 2842, 187). Ungewöhnlich früh hat er selbst Einfluss auf die bauliche Entwicklung der kriegszerstörten Stadt genommen, die er 1955 auch wegen der zunehmenden ideologischen Einengung verließ. Prof. em. Dr.-Ing. Dr. techn. h.c. mult. Walter Henn starb am 13. August 2006. Er war ein Architekt und Bauingenieur, der so vielfältig in Erscheinung trat: als einer der federführenden Architekten beim Wiederaufbau der TH Dresden nach 1945, der heutigen Technischen Universität, als Architekt zahlreicher Industrie- und Ingenieursbauten im In- und Ausland, als Wissenschaftler im disziplinären Austausch über politische Grenzen hinweg, als Vermittler zwischen den politischen Systemen, als Publizist.
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Truong, Phong Minh. "Hexa-aryl/alkylsubstituted Cyclopropanes." Digital Archive @ GSU, 2006. http://digitalarchive.gsu.edu/chemistry_theses/2.
Повний текст джерелаАнотація:
A series of penta-aryl/alkyl-1-(toluene-4-sulfonyl)-4,5-dihydro-1H-pyrazole 5a-c was synthesized by addition of methyllithium or phenylllithium followed by trapping the nitrogen anion intermediate with tosyl-fluoride to cyclic azines 2a,b. Addition of methyllithium or phenyllithium to 5a-c generated a series of hexa-aryl/alkylsubstituted-4,5-dihydro-3H-pyrazoles 6a-c. Neat thermolysis of hexa-aryl/alkylsubstituted-4,5-dihydro-3H-pyrazoles 6a-c at 200◦C produced hexa-aryl/alkylsubstituted cyclopropanes 7a-c in high yield.
19
Dugonic, Vesna. "TAKK för hela förskolan." Thesis, Malmö universitet, Fakulteten för lärande och samhälle (LS), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-33437.
Повний текст джерелаАнотація:
AbstractDenna studie handlar om TAKK (Tecken som alternativ och kompletterande kommunikation), en metod som används i den svenska förskolan för att stimulera barns kommunikation och språkutveckling. Ambitionen med studien är att undersöka TAKK-metoden som komplement till det verbala språket vid kommunikation och språkinlärning. Mer specifikt är syftet med studien att undersöka hur pedagoger använder, motiverar och värderar TAKK i arbete och utbildning.Examensarbete startade med en fördjupning i Vygotskijs teorier i det sociokulturella perspektivet på lärande. Studien baseras på data insamlad via semistrukturerade intervjuer med sammanlagt fem pedagoger från två olika förskolor, från en mellanstor stad i västra Sverige. Resultaten visar att båda förskolorna använde sig av TAKK som redskap för att stödja barns kommunikation och språkutveckling. De intervjuade pedagogerna var positivt inställda till användningen av TAKK-metoden och ansåg att TAKK kunde skapa en inkluderande miljö, där barn blir delaktiga i kommunikationen och lär sig tillsammans i samspel med pedagogerna och med varandra. Resultaten visar att pedagogernas användning av TAKK påverkas av utbildning och stöd, att det behövs både tid att lära sig tecken och att alla i arbetslaget är med för att pedagogerna skulle använda sig av TAKK aktivt i sitt arbete. Slutligen framkom det att TAKK används för hela barngrupper och i kommunikationen med alla barn på förskolorna, oavsett ålder eller kulturell bakgrund.
20
Xu, Duo, and Duo Xu. "Hera and Her Sanctuaries." Thesis, The University of Arizona, 2017. http://hdl.handle.net/10150/624103.
Повний текст джерелаАнотація:
Hera is among the most puzzling figures in Greek religion who has not been discussed fully in terms of the relationship between her personalities in myths, literature and art and her sanctuaries. Scholars have attempted to discern reasons behind the dichotomy that she was represented as a jealous wife and she was at the same time worshipped solemnly. But, these explanations are limited only to speculative evidences. Also, scholars, working on Hera, tend to focus on only one aspect of the goddess, whether it is archaeology, philology or religion.
I propose to conduct a holistic analysis of Hera, from her depictions in literature, art and myths to her major sanctuaries in the Greek world. I aim to look at multiple evidences: textual, iconographical, archaeological evidences. In short, the dichotomy of Hera helps consolidate the formation and the rising of Greek poleis, and it also empowers and encourages ancient Greek women to go through life crises.
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Adams, Markus. "Entwicklung eines Simulationsprogrammes für das Myon-Pretrigger-System des HERA-B-Experimentes und Untersuchungen zum Systemverhalten." [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=963995723.
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Stieler, Dirk. "Aufbau einer Triggerelektronik zur Messung der CP-Verletzung in B-Zerfällen bei HERA-B." [S.l.] : [s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=964146975.
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23
Cruse, Carsten. "Das RICH-Multiplizitätsveto-System für das HERA-B-Experiment." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=965529770.
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Trunk, Ulrich. "Development and characterisation of the radiation tolerant HELIX128-2 readout chip for the HERA-B microstrip detectors." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=961457392.
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25
İşsever, Selim. "Entwicklung des Target-Steuersystems für das HERA-B-Experiment und Untersuchung der Eigenschaften des Targetbetriebs am HERA-Speicherring." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=961866861.
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Borgmeier, Christoph. "Elektron- und Vertexrekonstruktion im frühen HERA-B-Detektor." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=960690409.
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Schreiner, Alexander. "Aging studies of drift chambers of the HERA-B outer tracker using CF4 -based gases." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=964245442.
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Keller, Stefan. "Aufbau eines Spurtriggers bei HERA-B mit GEM-MSGC-Detektoren." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=962686336.
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29
Schwenninger, Björn. "Das Myon-Pretrigger-System für das HERA-B-Experiment." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=962721522.
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30
Bräuer, Martin. "Die Alignierung des HERA-B-Vertexdetektors." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=963191187.
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31
Schaller, Sven. "Leistungsmerkmale des HERA-B-Vertexdetektors und Suche nach semileptonischen Charm-Zerfällen." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=963204025.
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32
Gradl, Sonja E. I. "Qualitätssicherung beim Bau des inneren Spurkammersystems von HERA-B und Studien zu einem Open-Charm-Trigger." [S.l. : s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=967807778.
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33
Jagla, Torsten. "Die Rohdatenüberwachung des Siliziumvertexdetektors von HERA-B und die A-Abhängigkeit des f-Produktionsquerschnitts [Phi-Produktionsquerschnitts] in Proton-Kern-Kollisionen bei 920-GeV-Protonenstrahlenergie." [S.l. : s.n.], 2003. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB10633940.
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34
Junghagen, Öberg Lena, and Tove Norkvist. "Vad hende sedan? : En studie om hen i gymnasieskolans svenskundervisning." Thesis, Umeå universitet, Institutionen för språkstudier, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-159193.
Повний текст джерелаАнотація:
This study focuses on the Swedish gender-neutral pronoun hen in upper-secondary Swedish teaching. Teachers were asked to participate in a survey with questions regarding hen to reveal if and how hen was included in their teaching in one way or another. It was also of interest to see what the teachers thought of hen in general and whether or not they used the word in their language. At the same time, certain parts were analysed in textbooks to see if hen was a part of the material that is distributed to the students. It was found that hen appears in textbooks, however, it was mostly in a meta-context and the textbooks did not have hen instead of double forms such as he/she. It was also found that most of the teachers thought of hen in a positive way, but the results show that teachers are more positive to the generic hen in comparison to the specific hen. A majority of the teachers in this study use hen but it is mostly within their work and not outside of school which indicates a moral usage rather than a natural usage. The results indicate that there is a lack of specific usage of hen in textbooks and also among teachers, but this may be due to the lack of transgender people. There is also a lack of natural usage of the generic hen in the textbooks instead of non gender-neutral pronouns.
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Yngvesson, Jenny. "Cannibalism in laying hens : characteristics of individual hens and effects of perches during rearing /." Skara : Dept. of Animal Environment and Health, Swedish Univ. of Agricultural Sciences ([Institutionen för husdjurens miljö och hälsa], Sveriges lantbruksuniv.), 2002. http://epsilon.slu.se/avh/2002/91-576-6360-2.pdf.
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Hillring, Björn. ""De ser hela barnet, hela dagen" : Fritidspedagogers uppdrag och ansvarsområden sett med rektorers glasögon." Thesis, Umeå universitet, Institutionen för tillämpad utbildningsvetenskap, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-121124.
Повний текст джерелаАнотація:
Syftet med denna studie var att erhålla kunskap om hur rektorer i grundskolan uppfattar fritidspedagogers ansvarsområden inom skolans verksamhet och det uppdrag som anges i styrdokumenten vad gäller barns och elevers tid i skola och fritidshem. Undersökningen utfördes genom att åtta rektorer intervjuades via semistrukturerade kvalitativa intervjuer med fenomengrafiska influenser i analysen. Studien visade på ett uppdrag som utifrån styrdokument bottnar i att se barnen och den grupp de är i och att arbeta med dem i olika sociala sammanhang. Typiska ansvarsområden som anges är delaktighet i samverkan med skolan på olika sätt och att föra dokumentation av verksamheten så den går att utvärdera och utveckla. I intervjuerna framkom att rektorerna ser fritidspedagogerna som kunniga inom området med grupporienterade processer och att de är duktiga på att komplettera skolan på olika sätt samt utgå från uppdraget som finns i styrdokumenten. Andra slutsatser var att det sytematiska kvalitetsarbetet är utmanande att hitta en bra systematik till och dokumentation avverksamheten kan bli bättre. Ytterligare en slutsats var att samverkan kan vara av olika karaktär, ofta är det som resurs fritidspedagogen samverkar.
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Tabet-Helal, Sana Zouleykha. "Impacts moléculaire et cellulaire de mutations de la partie N-terminale de la stathmine." Thesis, Evry-Val d'Essonne, 2014. http://www.theses.fr/2014EVRY0010/document.
Повний текст джерелаАнотація:
Les microtubules (MTs) sont des éléments majeurs du cytosquelette, ils participent à un ensemble de fonctions cellulaires d’importance comme la migration, le trafic des vésicules et des organelles ainsi qu’{ la division cellulaire au cours de laquelle ils forment le fuseau mitotique, permettant une ségrégation correcte des chromosomes. Ce sont des polymères cylindriques composés de protofilaments parallèles faits d’hétérodimères de tubulines α/β. Pour exercer ces fonctions, les MTs nécessitent une dynamique d’assemblage et de désassemblage régulée.La stathmine est une phosphoprotéine cytosolique de 17 kDa qui séquestre la tubuline dans un complexe non polymérisable formé de deux hétérodimères de tubuline par molécule de stathmine (complexe T2S). Les autres protéines de la famille stathmine (SCG10, SCLIP, RB3 et ses deux variants d’épissage RB3' et RB3″) forment aussi des complexes de séquestration appelés T2-SLD (Stathmin Like Domain) ayant des stabilités variables.En s’appuyant sur ces observations, des travaux ont montré qu’une chimère SN-CR constituée de la partie N-terminale de la stathmine et de la partie C-terminale du SLD de la protéine RB3 forme un complexe T2S remarquablement stable avec la tubuline (Jourdain et al., 2004). En parallèle à ces travaux, Clément et collaborateurs ont découvert que de courts peptides issus de la région N-terminale des SLD peuvent à eux seuls inhiber la polymérisation de la tubuline. Le peptide le plus efficace est le peptide I19L issu de la stathmine (peptide I19L (IQVKELEKRASGQAFELIL)). Il correspond à la région repliée en «β-hairpin» observée dans les cristaux T2S-SLD formés avec le domaine RB3-SLD (Clement et al., 2005). Pour augmenter l'efficacité de l’interaction entre les peptides issus de la région N-terminale des SLD et la tubuline, différentes mutations ont été introduites au niveau du peptide I19L. Les expériences de polymérisation in vitro de tubuline en présence d’une série de mutants de peptide I19L montrent que les peptides I19L-K4R et I19L-K4R-A10R sont les plus efficaces pour l’inhibition de l’assemblage de la tubuline.L’objectif de mes travaux { été d’introduire ces mutations au niveau de la partie N-terminale de la protéine stathmine et de la chimère NS-CR, afin d’analyser leurs efficacités sur l’inhibition de l’assemblage des MTs et la dynamique des MTs dans des cellules en culture de type HeLa, et de voir leur impact sur le cycle cellulaire.Mes résultats montrent que la stathmine mutant 2 (possédant les mutations K9R-A15R homologues aux mutations K4R-A10R du peptide I19L) induit une dépolymérisation des MTs dans des cellules en mitose par formation de fagots de MTs, réduit la dynamique de MTs et induit une cytotoxicité. L’ensemble des résultats suggère que nous pouvons améliorer la stabilité de liaison de la stathmine avec la tubuline par l’introduction de ces mutations, ce qui aurait in vitro un impact dans certains aspects cellulaires comme la prolifération cellulaire et la dynamique des MTs. Cela pourrait conduire à développer de nouvelles stratégies anticancéreuses visant à perturber le fuseau mitotiqueMicrotubules (MTs) are major constituents of the cytoskeleton and are involved in many cellular processes such as the determination of cell architecture, the formation of the mitotic spindle and intracellular trafficking. These tubular structures, composed by tubulin α/β heterodimers assemble and disassemble with a finely regulated dynamics.Stathmin is a cytosolic phosphoprotein that sequesters tubulin in a non polymerizable complex consisting of two tubulin heterodimers per stathmin molecule (T2S complex).The other stathmin family proteins (SCG10, SCLIP, RB3 and its two splice variants RB3 'and RB3 ") can also bind two tubulin heterodimers through their SLD (Stathmin Like Domain), but the different tubulin/SLD complexes display varying stabilities.Based on these observations, current works showed that one chimera protein consisting of the N-terminal domain of stathmin (NS) and of the C-terminal domain of RB3 (CR) forms a T2-SLD complex remarkably stable with tubulin (NS-CR chimera). Molecular simulation experiments in silico suggest that we can increase the affinity of the NS-CR chimera by introducing mutations in the NS subdomain (Jourdain et al., 2004).In parallel to this work, Clement and colleagues found that short peptides derived from the N-terminal region of SLD alone can inhibit tubulin polymerization. Among the N-terminal part of SLD, the most effective peptide is the peptide I19L (IQVKELEKRASGQAFELIL) derived from stathmin. It corresponds to region folded into a "β-hairpin" structure observed in the T2S crystals (Clement et al., 2005). To increase the efficiency of this interaction, different mutations were introduced at the level of the I19L peptide from the stathmin N-terminal domain and tested in vitro. The results showed that the peptides I19L-K4R and I19L-K4R-A10R are the most effective to in inhibit the assembly of MTs.In this work, we introduce these mutations in the N-terminal domains of stathmin and in the NS-CR chimera with the aim of analyzing their efficiencies on MTs disassembly in cancer cell cultures like HeLa, and the impact of the mutation on MTs dynamic and cell cycle.Results from overexpression experiments of WT and stathmin mutant 2 containing the double mutation (K9R-A15R similar to the mutations K4R-A10R for the I19L peptide), suggest that this mutant significantly induces depolymerization of mitotic MTs. In its less phosphorylated state on serine 16; this mutation also reduces MTs dynamics and induces cytotoxicity.We thus suggest that we can ameliorate the binding and efficiency of the N-terminal stathmin part by including this double mutation. This variation has an impact in vitro and in some cellular aspects such as cell proliferation and MTs dynamics. These findings may lead to a targeted therapy for this type of cancer
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Caron, Sascha. "Jets in photoproduction at HERA." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=96548209X.
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Lindskog, Ann-Sofie, and Ida Ringdahl. "Ovisshet upptar människans hela sfär." Thesis, Kristianstad University College, School of Health and Society, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:hkr:diva-5097.
Повний текст джерелаАнотація:
Bakgrund: Att inte veta hur saker och ting förhåller sig, är att sväva i ovisshet. Att leva i ovisshet och att inte veta hur ens framtid kommer att bli kan skapa funderingar som kan leda till olika reaktioner. Sådana funderingar och reaktioner blir ofta synliga i berättelser om det inträffade. En berättelse är en skildring av ett händelseförlopp muntligt eller skriftligt av verkliga eller påhittade händelser. Genom berättandet fångas helheten upp och återskapar upplevelser och känslor. Syfte: Syftet med studien var att få en ökad förståelse för fenomenet ovisshet. Metod: Studien genomfördes som en empirisk studie där studenters livsvärldsberättelser om fenomenet ovisshet utgjorde grunden för resultatet. Materialet har analyserats med kvalitativ innehållsanalys. Resultatet: Resultatet presenterades utefter tre frågor: 1 Vad innebär ovisshet? Tankar om framtiden och att inte ha makt att kunna påverka, 2 När uppkommer ovisshet? När det vardagliga bryts och 3 Vad gör ovisshet med människan? Känslor här och nu, tankar som svävar iväg. Diskussion: En ökad förståelse för vad ovisshet är underlättar för sjuksköterskan i hennes arbete att vårda och förstå den enskilde patientens livsvärld.
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Bergh, Nestlog Ewa. "Hela svenskan : Integrering inom svenskämnet." Thesis, Växjö University, School of Humanities, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:vxu:diva-1794.
Повний текст джерелаАнотація:
Syftet med denna uppsats är att undersöka en funktionell svenskundervisning där undervisning om språkliga strukturer integreras i undervisningen kring eget skrivande och analys av egna och andras texter. Fokus ligger på undervisningens relation till kontextuella aspekter av verksamheten, såsom sammanhang som har att göra med praxis i klassrummet, den enskilda skolan och yttre ramar på ett samhälleligt plan. Centrala begrepp är dialogicitet, processer, stödstrukturer, undervisning rörande språkliga strukturer, integrering, erfarenhetspedagogisk ämnessyn och text som socialt beteende. Såväl min analysapparat som den studerade undervisningen har inspirerats av ett sociokulturellt perspektiv på lärande, särskilt nydialogiska och genrepedagogiska teorier.
Studien följer fyra undervisningsförlopp i grundskolans senare år och i gymnasieskolan genom observationer, samtal i fokusgrupper samt intervjuer med lärare och elever.
Resultatet visar att den genomförda undervisningen värderas högt av lärare och elever, och lärarna menar att alla kategorier av elever tjänar på denna typ av integrerad undervisning. Den upplevs som effektiv och engagerande. Vad gäller de centrala begreppen, som nämnts ovan, kan man konstatera att det verkar ha funnits en strävan att implementera dessa i den studerade undervisningen, vilket också har skett i olika grad. En ytlig iscensättning av dessa begrepp i praktiken innebär dock knappast en undervisning som kan anses gestalta forskares teorier kring dessa begrepp. Ett flertal kontextuella aspekter på olika nivåer har identifierats som betydelsefulla i sammanhanget, som exempelvis läromedlens roll; lärarnas kunskaper och syn på lärande, på svenskämnet och på texter; lärarnas behov av forum för diskussioner och reflektion kring svenskundervisningen.
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Tuning, Niels. "Proton structure functions at HERA." [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2001. http://dare.uva.nl/document/85552.
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Cocks, Stuart Peter. "Rho prime electroproduction at HERA." Thesis, University of Liverpool, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366427.
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Bootman, Martin D. "Calcium spiking in HeLa cells." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.281898.
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Malmborg, Nordgren Camilla. "Viktigt på riktigt, hela dagen." Thesis, Malmö universitet, Fakulteten för lärande och samhälle (LS), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-33712.
Повний текст джерелаАнотація:
Syftet med denna studie har varit att undersöka barns inflytande och delaktighet vid en fri skapandesituation i ateljé och hur barn och pedagoger förhåller sig till varandra. Resultatet visar att pedagogerna har svårt att dela upp begreppen inflytande och delaktighet och använder dem synonymt. Pedagogerna är medvetna om att makt utövas men omedvetna om maktens olika nyanser. Studiens resultat visar på at det finns begränsningar i barns reella inflytande och delaktighet i den fria skapandesituationen där pedagogernas förhållningssätt speglar olika nyanser av maktutövningar.
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Uriondo, Boudri Heydy Wendy. "Estudios sobre la exportación de la proteína quinasa dependiente de cAMP en células humanas en cultivo." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2007. https://hdl.handle.net/20.500.12672/2515.
Повний текст джерелаАнотація:
La holoenzima proteína quinasa A (PKA) es un tetrámero que consiste en dos subunidades catalíticas y dos subunidades reguladoras, se encuentra en forma ubicua fosforilando un gran número de proteínas celulares y participa en la regulación de vías metabólicas y otros procesos (transporte de membrana, motilidad celular, expresión génica, apoptosis, aprendizaje y memoria).
Las proteínas quinasas no solamente se encuentran en el interior de las células, sino además se observa la "exportación" hacia la superficie de la cara externa de la membrana plasmática de las células (Redegeld, F.A et al., 1999). Este fenómeno de exportación de las proteína quinasas hacia la superficie de la cara externa de las células en cultivo se denomina "ectoquinasas."
El objetivo del presente trabajo es estudiar la exportación de la proteína quinasa dependiente del AMPc sobrexpresada en células humanas en cultivo a nivel intracelular o endógeno, a nivel ectópico o en la membrana plasmática, y libre en el líquido extracelular o proteína exógeno.
En la presente Tesis se utilizó dos líneas celulares llamadas HEK293T y HELA, realizándose la técnica de transfección por lipofectamina para introducir en estas células la proteína PKA, específicamente el vector (pcDNA3-HA) con el inserto que contiene el gen de la subunidad catalítica con el epítope anti-HA.Tesis
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Souza, Aryane Tofanello de [UNESP]. "Síntese e caracterização de nanopartículas magnéticas de óxido de ferro para aplicações biomédicas – um estudo citotóxico em linhagem celular de carcinoma cervical humano (células HeLa)." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/92023.
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000680523.pdf: 1511132 bytes, checksum: c20e5a6a65edf2cba90d4de2b5073a08 (MD5)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Nanopartículas magnéticas (NPMs) têm sido alvo de inúmeras investigações por seu grande potencial de aplicação nos mais diferentes campos tecnológicos. Dentre tantos, elas destacam-se na área biomédica, seja no diagnóstico ou tratamento de diversas doenças. Neste trabalho foram sintetizadas nanopartículas magnéticas (NPMs) de óxido de ferro (magnetita) pelo método de coprecipitação de íons Fe2+ e Fe3+ em meio alcalino. O objetivo central foi estudar as características morfológicas, estruturais, magnéticas e o comportamento biológico desses compostos em células cancerígenas, visando futuras aplicações biomédicas. Inicialmente as nanopartículas magnéticas foram avaliadas em função dos parâmetros físico-químicos que influenciam diretamente as características finais do produto (pH, molaridade, temperatura e tipo de base) para se observar as melhores condições de síntese e a influência de cada um nas características do produto. As nanopartículas foram caracterizadas por difratometria de raios-X, microscopia eletrônica de varredura, potencial zeta e magnetometria. Desse primeiro estudo concluiu-se que a coprecipitação produz partículas com polidispersão de tamanhos alta e que os parâmetros de síntese influenciam drasticamente as propriedades dos materiais, no entanto, todas as amostras exibiam características magnéticas. Depois de estabelecida esta etapa, as NPMs foram submetidas à transfecção em cultura celular de carcinoma cervical humano (células HeLa) e a testes biológicos como coloração com Azul da Prússia e hematoxilina-eosina, ensaio de MTT e ensaio de apoptose para averiguação da citotoxidade. A principal observação vinda desses resultados foi que as nanopartículas magnéticas sintetizadas, salvo algumas adaptações de síntese...
Magnetic nanoparticles (MNPs) have been the subject of numerous investigations because of its great potential application in many different fields of technology. Among many, they stand out in the biomedical area, either in diagnosis or treatment of various diseases. In this work were synthesized magnetic nanoparticles (MNPs) of iron oxide (magnetite) by the coprecipitation method of Fe2+ and Fe3+ in an alkaline medium. The main objective was to study the morphological, structural, magnetic and biological behavior of these compounds in cancer cells, in order to future biomedical applications. Initially the magnetic nanoparticles were evaluated against the physical and chemical parameters that directly influence the final characteristics of product (pH, molarity, temperature and type of base) to observe the best synthesis conditions and influence of each characteristics. The nanoparticles were characterized by X-ray diffraction, sccaning electron microscopy, zeta potential and magnetometry. In this first study showed that coprecipitation produces particles with high polydispersity of sizes and that the synthesis parameters dramatically influence the properties of materials, however all samples exhibited magnetic characteristics. After you make this step, the MNP were subjected to transfection in cell culture of human cervical carcinoma (HeLa) and biological tests such as staining with Prussian blue and hematoxylin-eosin, MTT assay and apoptosis assay to investigate cytotoxicity. The main observation was that these results coming magnetic nanoparticles synthesized, except for some adjustments in short, constitute a class of nanocomposites with enormous potential for therapeutic and / or diagnosis. The work brings together information ranging from the synthesis of nanoparticles to their behavior inside the cells, emphasizing the best conditions for each procedure
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Diniz, Silvana. "Os santuários de Hera enquanto elementos do espaço políade." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/71/71131/tde-18102011-091535/.
Повний текст джерелаАнотація:
As últimas décadas têm testemunhado contínuos debates acerca dos papéis que os santuários desempenhavam para a formação e desenvolvimento das pólis e das comunidades gregas em geral. As políticas de posicionamento dos santuários, questão esta vinculada com as motivações e interesses para uma dada divindade ser cultuada em determinado local, têm sido objetos de várias discussões. Assim, selecionamos um grupo de santuários dedicados a Hera (chamados Heraia/ sing. Heraion), de modo a analisá-los enquanto contextualizados nas respectivas comunidades a que cada um pertencia, dentro de um recorte cronológico que vai do séc. VIII ao séc. V. Os Heraia em questão situam-se em Samos, Corinto (Peracora), Argos, Metaponto, Crotona, Poseidônia e Selinonte. Para a condução da pesquisa, focalizamos as funções dos santuários de Hera e as esferas de atuação da deusa em relação às pólis pertinentes. Igualmente, consideramos os aspectos espaciais dos Heraia e das pólis. Enfim, procedemos à comparação com base em alguns critérios gerais que escolhemos. Valemo-nos de uma ampla variedade de documentação, sobretudo arqueológica, não desconsiderando, entretanto, as contribuições que os textos antigos puderam trazer. O estudo dos Heraia que realizamos permitiu que constatássemos, a partir de casos específicos, a multiplicidade de funções e papéis dos santuários no mundo grego. Enfim, nossa pesquisa contribuiu para entender o culto de uma divindade como fenômeno histórico engastado em práticas sociais.The past decades have witnessed ongoing debates on the roles Greek sanctuaries exerted on the formation and development of the polis and the Greek communities in general. Sanctuary placement policies- an issue that is also related to the motivations and interests backing the location of the cult to a given deity- are also themes that have drawn the attention of several scholars. Thus we picked a group of sanctuaries dedicated to Hera (called Heraia/ sing. Heraion), so as to analise them as contextualised and embedded in their respective communities to which they belonged, within a period that goes from the VIII century up to the V century. The Heraia in question were located in Samos, Corynth (Perachora), Argos, Metaponto, Croton, Poseidonia and Selinonte. In order to carry out this research, we focused on the functions of Hera´s sanctuaries as well as on the the actuation spheres of this goddess as far as the polis are concerned. Likewise, we payed due regard for the spatial aspects of the Heraia. Finally, we proceeded to a comparison according to some criteria we selected. We made use of a broad range of documentation, especially archaeological sources, also considering the contributions the ancient texts could make. The study of the sanctuaries of Hera we conducted enabled us to notice the multiplicity of functions and roles of sanctuaries across the Greek world. Therefore, our research contributed to the understanding of the cult of a Greek deity as a historical phenomenon embedded in social practices.
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Oliveira, Ruth Medeiros de. "Avalia??o das atividades antioxidante, anticoagulante e antiproliferativa de extratos aquosos de marsdenia megalantha." Universidade Federal do Rio Grande do Norte, 2011. http://repositorio.ufrn.br:8080/jspui/handle/123456789/12576.
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The species of the genus Marsdenia, Apocynaceae, are widely used in folk medicine of several countries. In Brazil is found several species belonging to this genus. The in vitro antioxidant, anticoagulant and antiproliferative activities were evaluated to aqueous extracts of stalk, leaf and root of Marsdenia megalantha. In the total antioxidant capacity assay (expressed as ascorbic acid equivalents) the stalk extract showed 76.0 mg/g, while leaf and root extracts 141.3 mg/g and 57.0 mg/g, respectively. The stalk and leaf extracts showed chelating activity around 40% at 1.5 mg/mL, while root extract, at the same concentration showed, 17%. Only the leaf extract showed a significant ability in superoxide scavenging (80% at 0.8 mg/mL). Any extract was able in scavenge hydroxyl, as well anticoagulant activity. The antiproliferative activity of the extracts was evaluated against HeLa tumor cell line. The extracts inhibited in a dose-dependent manner the cell growth. However, the leaf extract showed 80% of inhibition at 1.0 mg/mL, while stalk and root extracts inhibited 63% and 30%, respectively. To assess the mechanism of cell death caused by the leaf extract in HeLa, was performed flow cytometry and western blot. The results show that leaf extract induces cell death by apoptosis through an activation caspase-independent pathway. These data indicate that stalk and leaf extracts obtained have potential to be used as antioxidants and anticancer drugs
As esp?cies do g?nero Marsdenia, Apocynaceae, s?o bastante utilizadas na medicina popular de v?rios pa?ses. No Brasil s?o encontradas v?rias esp?cies pertencentes a esse g?nero. As atividades antioxidante, anticoagulante e antiproliferativa foram avaliadas para os extratos de caule, folha e raiz de Marsdenia megalantha. Na capacidade antioxidante total (expressa como equivalente de ?cido asc?rbico), o extrato do caule apresentou 76,0 mg/g, enquanto os extratos da folha e raiz apresentaram, respectivamente, 14,3 mg/g e 57,0 mg/g. Os extratos do caule e da folha mostraram habilidade quelante em torno de 40% na concentra??o de 1,5 mg/mL, enquanto o extrato da raiz, na mesma concentra??o, apresentou 17%. Apenas o extrato da folha apresentou uma capacidade significante em sequestrar radicais super?xidos (80% em 0,8 mg/mL). Nenhum extrato mostrou capacidade em seq?estrar radicais hidroxila, bem como atividade anticoagulante. A atividade antiproliferativa dos extratos foi avaliada contra a linhagem tumoral HeLa. Os extratos inibiram, de forma dose-dependente, o crescimento celular. Entretanto, o extrato da folha foi capaz de inibir em 80% a prolifera??o celular na concentra??o de 1,0 mg/mL, enquanto os extratos de caule e raiz inibiram 63% e 30%, respectivamente. Para avaliar o mecanismo de morte celular causada pelo extrato da folha nas c?lulas HeLa, foi realizada citometria de fluxo e western blot. Os resultados mostraram que o extrato da folha induz morte celular por apoptose atrav?s de uma via de ativa??o independente de caspase. Estes resultados indicam que os extratos de caule e folha obtidos t?m potencial para serem futuramente utilizados como f?rmacos antioxidantes e antic?ncer
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Souza, Aryane Tofanello de. "Síntese e caracterização de nanopartículas magnéticas de óxido de ferro para aplicações biomédicas - um estudo citotóxico em linhagem celular de carcinoma cervical humano (células HeLa) /." São José do Rio Preto : [s.n.], 2011. http://hdl.handle.net/11449/92023.
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Orientador: José Geraldo NeryBanca: Paula Rahal
Banca: Maria Cristina Nonato Costa
Resumo: Nanopartículas magnéticas (NPMs) têm sido alvo de inúmeras investigações por seu grande potencial de aplicação nos mais diferentes campos tecnológicos. Dentre tantos, elas destacam-se na área biomédica, seja no diagnóstico ou tratamento de diversas doenças. Neste trabalho foram sintetizadas nanopartículas magnéticas (NPMs) de óxido de ferro (magnetita) pelo método de coprecipitação de íons Fe2+ e Fe3+ em meio alcalino. O objetivo central foi estudar as características morfológicas, estruturais, magnéticas e o comportamento biológico desses compostos em células cancerígenas, visando futuras aplicações biomédicas. Inicialmente as nanopartículas magnéticas foram avaliadas em função dos parâmetros físico-químicos que influenciam diretamente as características finais do produto (pH, molaridade, temperatura e tipo de base) para se observar as melhores condições de síntese e a influência de cada um nas características do produto. As nanopartículas foram caracterizadas por difratometria de raios-X, microscopia eletrônica de varredura, potencial zeta e magnetometria. Desse primeiro estudo concluiu-se que a coprecipitação produz partículas com polidispersão de tamanhos alta e que os parâmetros de síntese influenciam drasticamente as propriedades dos materiais, no entanto, todas as amostras exibiam características magnéticas. Depois de estabelecida esta etapa, as NPMs foram submetidas à transfecção em cultura celular de carcinoma cervical humano (células HeLa) e a testes biológicos como coloração com Azul da Prússia e hematoxilina-eosina, ensaio de MTT e ensaio de apoptose para averiguação da citotoxidade. A principal observação vinda desses resultados foi que as nanopartículas magnéticas sintetizadas, salvo algumas adaptações de síntese... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Magnetic nanoparticles (MNPs) have been the subject of numerous investigations because of its great potential application in many different fields of technology. Among many, they stand out in the biomedical area, either in diagnosis or treatment of various diseases. In this work were synthesized magnetic nanoparticles (MNPs) of iron oxide (magnetite) by the coprecipitation method of Fe2+ and Fe3+ in an alkaline medium. The main objective was to study the morphological, structural, magnetic and biological behavior of these compounds in cancer cells, in order to future biomedical applications. Initially the magnetic nanoparticles were evaluated against the physical and chemical parameters that directly influence the final characteristics of product (pH, molarity, temperature and type of base) to observe the best synthesis conditions and influence of each characteristics. The nanoparticles were characterized by X-ray diffraction, sccaning electron microscopy, zeta potential and magnetometry. In this first study showed that coprecipitation produces particles with high polydispersity of sizes and that the synthesis parameters dramatically influence the properties of materials, however all samples exhibited magnetic characteristics. After you make this step, the MNP were subjected to transfection in cell culture of human cervical carcinoma (HeLa) and biological tests such as staining with Prussian blue and hematoxylin-eosin, MTT assay and apoptosis assay to investigate cytotoxicity. The main observation was that these results coming magnetic nanoparticles synthesized, except for some adjustments in short, constitute a class of nanocomposites with enormous potential for therapeutic and / or diagnosis. The work brings together information ranging from the synthesis of nanoparticles to their behavior inside the cells, emphasizing the best conditions for each procedure
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Zhang, Xin. "Social transformation in modern China : the state and local elites in Henan, 1900-1937 /." Cambridge : Cambridge university press, 2000. http://catalogue.bnf.fr/ark:/12148/cb38889393b.
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