Дисертації з теми "Hematologic neoplasm"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-28 дисертацій для дослідження на тему "Hematologic neoplasm".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте дисертації для різних дисциплін та оформлюйте правильно вашу бібліографію.
Holgersson, Åsa. "DNA-dependent protein kinase in normal and malignant cells : with special reference to anti-tumour agent sensitivity /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-431-3/.
Повний текст джерелаEngel, Roxane. "The nuclear export of DNA topoisomerase iialpha in hematological myeloma cell lines as a function of drug sensitivity : clinical implications and a theoretical approach for overcoming the observed drug resistance /." [Tampa, Fla.] : University of South Florida, 2005. http://purl.fcla.edu/fcla/etd/SFE0001358.
Повний текст джерелаCherif, Honar. "Infections in patients with hematological malignancies : etiology, trends and management /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-501-1/.
Повний текст джерелаSousa, Renata Miranda de. "Atenção integral ao paciente onco-hematológico hospitalizado: proposta de um protocolo de cuidados de enfermagem." Universidade Federal Fluminense, 2013. https://app.uff.br/riuff/handle/1/1105.
Повний текст джерелаMade available in DSpace on 2015-12-09T16:36:13Z (GMT). No. of bitstreams: 1 Renata Miranda de Sousa.pdf: 3625328 bytes, checksum: 0b31c5852dae5a67886b1a2736d27622 (MD5) Previous issue date: 2013
Mestrado Acadêmico em Ciências do Cuidado em Saúde
Trata-se de uma pesquisa qualitativa, descritiva do tipo estudo de caso único, que teve como objeto de estudo cuidados de enfermagem aos pacientes onco-hematológicos hospitalizados e os seguintes objetivos: caracterizar o perfil dos pacientes onco-hematológicos hospitalizados na enfermaria de hematologia do Hospital Universitário Antônio Pedro (HUAP), identificar as demandas de cuidado dos pacientes onco-hematológicos hospitalizados na enfermaria de hematologia do HUAP, descrever os cuidados realizados pela equipe de enfermagem aos pacientes onco-hematológicos hospitalizados na enfermaria de hematologia do HUAP, elaborar um protocolo de cuidados de enfermagem aos pacientes onco-hematológicos hospitalizados na enfermaria de hematologia do HUAP, a partir dos relatos dos membros da equipe de enfermagem. O cenário de pesquisa foi à unidade de hematologia do HUAP. Fizeram parte do estudo 17 sujeitos, sendo sete (7) pacientes hospitalizados com diagnóstico médico de LMA, LMC, LLA, LLC, Linfoma de Hodgkin e Não-Hodgkin e dez (10) membros da equipe de enfermagem. A coleta de dados foi através do formulário de identificação e o histórico de enfermagem com os pacientes e a observação participante acerca do cuidado de enfermagem ao paciente onco-hematológico hospitalizado e as entrevistas com a equipe de enfermagem. Os resultados apontaram como demandas de cuidado de enfermagem àqueles relacionados à atenção, cuidados gerais, ambiente de cuidado; orientação à família e ao paciente e equipe especializada. Com relação ao cuidado que a equipe de enfermagem realiza foram identificados: cuidado prevenção; cuidado sensível; cuidado clínico; cuidado atributos; cuidado educativo. E o cuidado específico ao paciente onco-hematológico está atrelado à prevenção, conforto, apoio emocional e orientação e tais cuidados foram os subsídios para a elaboração do protocolo de cuidado de enfermagem a estes pacientes segundo a realidade local do HUAP. Desta forma, tal protocolo consolida o que a enfermagem do serviço de hematologia do HUAP já vem realizando. Além disso, torna-se aos membros desta equipe um apoio na prestação da assistência de enfermagem de modo que possam identificar no protocolo os cuidados específicos direcionados as demandas de cuidados destes pacientes.
This is a qualitative, descriptive research of type single case study, which had as its object of study nursing care to onco-hematological patients hospitalized and the following goals: characterize the profile of onco-hematological patients hospitalized in hematology ward of the University Hospital Antônio Pedro (HUAP), identifying the demands of care of oncohematological patients hospitalized in hematology ward of HUAP, describing the care provided by nursing staff to onco-hematological patients hospitalized in hematology ward of HUAP, draw up a protocol of nursing care to onco-hematological patients hospitalized in hematology ward of HUAP, from reports of the members of the nursing staff. The scenario of research was the Hematology unit of HUAP. Were part of the study 17 subjects, being seven (7) hospitalized patients with medical diagnosis of LMA, LMC, LLA, LLC, Hodgkin's lymphoma and non-Hodgkin lymphoma and ten (10) members of the nursing staff. The data collection was through the identification form and the nursing history with patients and the participant observation about nursing care to the onco-hematological patient hospital and interviews with the nursing staff. The results showed how nursing care as demands related to attention, general care, environment of care; guidance to the family and the patient and expert staff. With respect to the care that nursing staff conducts were identified: preventive care; sensitive care; clinical care; careful attributes; educational care. And patient specific oncohematological care is related to prevention, comfort, emotional support and guidance and such care was the allowances for nursing care protocol to these patients according to the local reality of HUAP. In this way, such a protocol that consolidates what the Hematology nursing service of HUAP already has been performing. Moreover, it becomes to members of this team a support in the provision of nursing care so that they can identify the protocol specific targeted care demands these patients.
Duda, Naila Cristina Blatt. "Anormalidades hematológicas, bioquimicas e hemostáticas de origem paraneoplásica em fêmeas caninas com neoplasia mamária." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/96929.
Повний текст джерелаHematological abnormalities of paraneoplastic origin are identified in several types of cancers that affect dogs and cats. In dogs with mammary neoplasms, abnormalities associated with coagulation have been identified, and verified that disseminated intravascular coagulation (DIC) clinical and subclinical may be present in 83% of dogs with mammary carcinoma. In human medicine, research in this field has been relevant since those factors are indicators of cancer prognosis. Meanwhile, in veterinary medicine, there are few studies that relate hematological changes with tumor type, staging and determination of prognosis. The aim of this study was to evaluate the hematological, biochemical and hemostathic abnormalities in bitches affected by mammary cancer to identify the most frequent alteration and associate with tumor staging. For this, 25 bitches attended by the Oncology Study Group in small animals (ONCOVET) of the Veterinary Hospital of UFRGS (HCV-UFRGS) for the period of 4 months were used. Blood collection for complete blood count, platelet count, serum biochemistry (albumin, ALT, calcium, creatinine, ALP, glucose, urea) and coagulation test that consisted of PT (prothrombin time), aPTT (activated partial thromboplastin time), TT (thrombin time), fibrinogen and D-dimer measurement were performed. Tumor staging was obtained by physical examination and the results of the biopsy of the breast. The abnormalities found included anemia, neutrophilic leukocytosis, monocytosis, eosinophilia, thrombocytosis, hypoalbuminemia, hypocalcemia, hypoglycemia and decreased levels of urea. However, these changes were not associated directly with tumor progression, since there was no difference among the groups. Only the RDW and ALT variables was associated significantly between the groups, however, with no clinical relevance. In the coagulation test, there was significant difference between the groups only in TT and fibrinogen, which was associated with tumor staging.
Malec, Maria. "Standardization and application of quantitative PCR methods in patients with hematological malignancies /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7140-099-0/.
Повний текст джерелаPersson, Lennart. "Neutropenic fever during treatment of hematological malignancy : etiology and diagnostics /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-194-6/.
Повний текст джерелаJoão, Cristina Maria Pires. "Immune reconstitution after autologous hematopoietic stem cell transplantation." Doctoral thesis, Faculdade de Ciências Médicas. Universidade Nova de Lisboa, 2007. http://hdl.handle.net/10362/5158.
Повний текст джерелаÖhrn, Kerstin. "Oral health and experience of oral care among cancer patients during radio- or chemotherapy /." Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2001. http://publications.uu.se/theses/91-554-4940-9/.
Повний текст джерелаSöderberg, Karin. "Risk factors for haemagological malignancies : immune-mediated diseases, body mass index and magnetic fields /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-819-3/.
Повний текст джерелаCantu, Silvana Oliveira [UNESP]. "Avaliação da intervenção psicoterapêutica de modelo analitico em crianças e adolescentes com doenças onco-hematológicas." Universidade Estadual Paulista (UNESP), 2010. http://hdl.handle.net/11449/88104.
Повний текст джерелаFundação para o Desenvolvimento Médico e Hospitalar (Famesp)
O processo de adoecer provoca uma crise vital para a pessoa. Na criança traz repercussões mais sérias, pois os conteúdos do inconsciente estão ainda pouco discriminados pelo ego e nesta situação pela percepção da possibilidade de desintegração ou morte, alguns destes conteúdos tornou-se mais intrusos e ameaçadores. Enfermidades graves durante a infância ou na adolescência tais como, as neoplasias, doenças cardíacas, insuficiência renal, diabetes, doenças com má formação genéticas, doenças hematológicas, dentre outras, conduzem o paciente e seus familiares a situações de crise, manifesta por estresse emocional e comportamental, perturbações da auto-imagem e auto-estima, comprometimento da qualidade de vida, dentre outros sintomas psicopatológicos. O objetivo deste trabalho foi o de avaliar a intervenção psicoterápica em modelo analítico em crianças e adolescentes com doenças onco-hematológicas. Foram utilizados dois instrumentos de avaliação: inventários de estresse e depressão infantil (ISI e CDI) aplicados em 4 diferentes momentos após a primeira avaliação: TO, T30, T60 , T90 e T120. Foram acompanhados 7 pacientes do ambulatório de psicologia OEI do Hemocentro de Marília. Trata-se de um ensaio clínico não-randomizado intra-grupos de séries temporais, no qual a intervenção foi realizada com um único grupo e os desfechos foram comparados em cada participante durante diferentes períodos desta intervenção. A análise dos escores CDI ao final do período das intervenções observou-se uma melhora no estado geral dos sintomas de depressão dos participantes (escores finais aos 120 dias inferiores a 17) comprovando a eficácia da intervenção psicoterápica de base analítica na melhora dos sintomas em crianças e adolescentes portadores de neoplasias onco-hematológicos. Os dados...
The process of getting sick causes a vital crisis to the person. It brings more severe repercussions to the children since the unconscious contents are hardly discriminates by the ego and, in this special situation, by the perception of the possibility of disintegration or even death. So some of these contents become more threatening. Serious diseases during childhood or adolescence such as neoplasi, cardio illness, kidney insufficiency, diabetes, genetics and blood problems, among others, lead the patient and his family members to crises with emotional and behavior stress, self-esteem disturbance, even involving the daily life and other psychopathic symptoms. The objective on this paper was to evaluate a psychotherapeutic intervention in an analytical pattern for children and teenagers with onco-hemathology diseases. Two models for evaluation were used: Stress inventory and depression on in children (ISI and CDI) applied in four different times after the first evaluation: TO, T30, T60, T90 and T12. Seven patients of the psychology ambulatory OEI in the Hemocentro in Marilia were watched. It is a clinic essay not at random among groups on specific times in which the intervention was accomplished with a unique group and the results were compared on each participant in different times. In the analyses of the scores CDI in the end of interventions, some improvements in the overall depression symptoms were clearly noticed (final scores in 120 days bellow 17) proving the effectiveness of the psychotherapeutic intervention based on the analysis of the symptoms in kids and teen suffering from neoplasi and oncohematology diseases. The inventory data inn children stress are similar to the depression pointing to the effectives of the psychotherapeutic approach. The variations of the physical reactions (ESI) are related to the... (Complete abstract click electronic access below)
Moraes, José Renato de 1976. "O ar como fonte ambiental para fusariose sistêmica em pacientes receptores de células-tronco hematopoéticas e doenças onco-hematológicas internados no Hospital de Clínicas - UNICAMP." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/311063.
Повний текст джерелаDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
Made available in DSpace on 2018-08-27T08:47:24Z (GMT). No. of bitstreams: 1 Moraes_JoseRenatode_M.pdf: 2487255 bytes, checksum: 4c5a21c13a0b57cf864dbf79cca990fd (MD5) Previous issue date: 2014
Resumo: Introdução: os fungos do gênero Fusarium são ubíquos no meio ambiente e causam doenças em plantas e aos seres humanos. Objetivos: 1)estabelecer o protocolo para a coleta e o isolamento de Fusarium spp. no ar; 2)isolar fungos do gênero Fusarium do ar da enfermaria de Hematologia e da Unidade de Transplante de Medula Óssea (TMO) no Hospital de Clínicas (HC) da Universidade Estadual de Campinas (UNICAMP) e comparar a relação genética de isolados ambientais de Fusarium spp. com os isolados a partir de hemoculturas de rotina diagnóstica de pacientes hospitalizados durante os anos de 2002 a 2013, 3)identificar os fungos do gênero Fusarium ao nível de complexo de espécies por PCR em tempo real, e ao nível de espécie pelo sequenciamento de fragmento do gene EF1?. Metodologia: foram colhidas amostras de ar nas unidades de TMO (que possui controle de ar através de filtros HEPA e fluxo de ar de pressão positiva) e na Hematologia (unidade sem controle do ar) do HC. De 2006 a 2013 foram identificados 18 isolados de Fusarium spp. em culturas de sangue. A coleta de ar foi realizada através do amostrador de ar BioSamp modelo MBS 1000D (YotsubishiCorp Japão). Um meio de cultura seletivo para Fusarium (Agar Komada com modificações) foi usado na placa de Petri dentro do amostrador de ar. O volume de ar de 1000 mL e 500 mL foram aspirados da Hematologia e enfermarias de TMO, respectivamente. Após o tempo de incubação, as colônias que cresceram nas placas foram avaliadas macroscopicamente e microscopicamente para identificação morfológica. Fusarium spp. foram identificados através de um novo ensaio de PCR em tempo real composto por dois ensaios de PCR em tempo real: um específico para o gênero Fusarium e um ensaio específico para detecção específica do complexo de espécies de Fusarium solani (FSSC). Para confirmar o complexo de espécies de Fusarium, uma região parcial do gene EF-1? foi sequenciado. O alinhamento das sequências foi realizado no programa Clustal W e a análise filogenética utilizou o programa Mega5 com o algorítimo Neighbor-Joininge Bootstrap de 1000 replicatas. Resultado: foram isoladas 108 cepas de Fusarium spp. destes 28% na unidade de TMO e 78% na Hematologia. Os complexos de espécies encontrados foram: 10% FCSC, 21% FIESC, 1% FOSC, 12% FSSC, 51% GFSC e 5% não definido. Na TMO a maior prevalência foi da espécie Fusarium solani (9/30 isolados) e na Hematologia Fusarium verticillioides (25/31 isolados). Não foi possível constatar relevância entre a temperatura e umidade do ar em relação ao aumento ou diminuição de isolados nas coletas. Nove isolados FSSC do ar e 8 de sangue apresentaram BT 99%. Conclusão. Os dados de análise filogenética sugeriram que os isolados de sangue de pacientes e ambientais foram similares sugerindo que o ambiente pôde representar uma fonte potencial de infecção para os pacientes imunodeprimidos
Abstract: Introduction:the fungus Fusarium are ubiquitous in the environment and cause diseases in plants and humans. Objectives: isolate fungi Fusarium in the air of the ward Hematology and Unit of Bone Marrow Transplantation (BMT) at the Hospital de Clinicas (HC), State University of Campinas (UNICAMP), compare the phylogenetic relationship of environmental isolates of Fusarium spp .with isolates from blood cultures of routine diagnosis of hospitalized patients during the years 2002-2013, to establish protocol for the collection and isolation of Fusarium spp. in the air, identify the fungi Fusarium species complex level by real time PCR, and to species level by sequencing the fragment EF1 ? gene. Methodology: air sample was collected in BMT units (which has control of air through HEPA filters and air flow positive pressure) and Hematology (there is not self control air inlet) HC. From 2006 to 2013, 18 samples obtained from blood cultures with the presence of Fusarium spp. were added to study the relationship between degree of molecular clinical isolates isolated from the air. The air sampling was performed using the Bio air sampler Samp MBS model 1000D (Yotsubishi Corp. Japan). A selective medium for Fusarium (Komada Agar changes by Y. Mikami, Chiba University) was used in the petri dish in the air sampler. The air volume of 1000 mL and 500 mL were aspirated Hematology and BMT wards, respectively. After the incubation time, colonies that grew on the plates were evaluated macroscopically and microscopically for morphological identification. Fusarium spp. were identified by PCR of a new system in real time according to Muraosa et al. comprising two PCR assays in real time: the specific assay Fusarium and a specific assay for specific detection of Fusarium solani (FSSC) complex. To confirm the complex of Fusarium species, a partial region of the EF-1? gene was sequenced. The alignment of sequences was performed in Clustal W program and phylogenetic analysis used Mega5 program with the neighbor-joining algorithm and bootstrap 1000 replicates. Results: 108 ceepas Fusarium spp were isolated. 28% of these isolates form the BMT unit and 78% in Hematology. Overall the species complexes ficarm divided as follows: 10% FCSC, 21% FIESC 1% FOSC, FSSC 12%, 51% and 5% GFSC nd not defined). In the BMT was higher prevalence of the species Fusariu solani (9/30 isolates) and the Hematology espéciecom largest presence is Fusarium verticillioides (25/31 isolates). Unable to find relevance between temperature and air humidity in relation to the increase or decrease in collections of isolates. Nine isolates of Fusarium solani air obtained from the same source ancestras 8 Fusarium solani isolates from blood (BT 99%). Conclusion:Fusarium spp were isolated in 108 environmental samples en all evaluated environments. Phylogenetically findings suggest equivalence between environmental isolates and isolates from the blood of patients. The collection protocol set showed satisfactory for the specific isolation of Fusarium result. The findings of Fusarium were grouped into complexes FSSC and FSSC not the technique of real-time PCR and sequencing of the gene EF1? allowed to classify the isolates in the species level
Mestrado
Clinica Medica
Mestre em Ciências
Berard, Raymond. "Psychiatric aspects of haematological malignant disease : the Groote Schuur experience." Master's thesis, University of Cape Town, 1992. http://hdl.handle.net/11427/25946.
Повний текст джерелаWatatani, Yosaku. "Molecular heterogeneity in peripheral T-cell lymphoma, not otherwise specified revealed by comprehensive genetic profiling." Kyoto University, 2020. http://hdl.handle.net/2433/253204.
Повний текст джерелаCantu, Silvana Oliveira. "Avaliação da intervenção psicoterapêutica de modelo analitico em crianças e adolescentes com doenças onco-hematológicas /." Botucatu : [s.n.], 2010. http://hdl.handle.net/11449/88104.
Повний текст джерелаBanca: Roseli Nunes Antunes
Banca: Juliana Bassalobre Carvalho Borges
Resumo: O processo de adoecer provoca uma crise vital para a pessoa. Na criança traz repercussões mais sérias, pois os conteúdos do inconsciente estão ainda pouco discriminados pelo ego e nesta situação pela percepção da possibilidade de desintegração ou morte, alguns destes conteúdos tornou-se mais intrusos e ameaçadores. Enfermidades graves durante a infância ou na adolescência tais como, as neoplasias, doenças cardíacas, insuficiência renal, diabetes, doenças com má formação genéticas, doenças hematológicas, dentre outras, conduzem o paciente e seus familiares a situações de crise, manifesta por estresse emocional e comportamental, perturbações da auto-imagem e auto-estima, comprometimento da qualidade de vida, dentre outros sintomas psicopatológicos. O objetivo deste trabalho foi o de avaliar a intervenção psicoterápica em modelo analítico em crianças e adolescentes com doenças onco-hematológicas. Foram utilizados dois instrumentos de avaliação: inventários de estresse e depressão infantil (ISI e CDI) aplicados em 4 diferentes momentos após a primeira avaliação: TO, T30, T60 , T90 e T120. Foram acompanhados 7 pacientes do ambulatório de psicologia OEI do Hemocentro de Marília. Trata-se de um ensaio clínico não-randomizado intra-grupos de séries temporais, no qual a intervenção foi realizada com um único grupo e os desfechos foram comparados em cada participante durante diferentes períodos desta intervenção. A análise dos escores CDI ao final do período das intervenções observou-se uma melhora no estado geral dos sintomas de depressão dos participantes (escores finais aos 120 dias inferiores a 17) comprovando a eficácia da intervenção psicoterápica de base analítica na melhora dos sintomas em crianças e adolescentes portadores de neoplasias onco-hematológicos. Os dados... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The process of getting sick causes a vital crisis to the person. It brings more severe repercussions to the children since the unconscious contents are hardly discriminates by the ego and, in this special situation, by the perception of the possibility of disintegration or even death. So some of these contents become more threatening. Serious diseases during childhood or adolescence such as neoplasi, cardio illness, kidney insufficiency, diabetes, genetics and blood problems, among others, lead the patient and his family members to crises with emotional and behavior stress, self-esteem disturbance, even involving the daily life and other psychopathic symptoms. The objective on this paper was to evaluate a psychotherapeutic intervention in an analytical pattern for children and teenagers with onco-hemathology diseases. Two models for evaluation were used: Stress inventory and depression on in children (ISI and CDI) applied in four different times after the first evaluation: TO, T30, T60, T90 and T12. Seven patients of the psychology ambulatory OEI in the Hemocentro in Marilia were watched. It is a clinic essay not at random among groups on specific times in which the intervention was accomplished with a unique group and the results were compared on each participant in different times. In the analyses of the scores CDI in the end of interventions, some improvements in the overall depression symptoms were clearly noticed (final scores in 120 days bellow 17) proving the effectiveness of the psychotherapeutic intervention based on the analysis of the symptoms in kids and teen suffering from neoplasi and oncohematology diseases. The inventory data inn children stress are similar to the depression pointing to the effectives of the psychotherapeutic approach. The variations of the physical reactions (ESI) are related to the... (Complete abstract click electronic access below)
Mestre
Caetano, Fabiane Bis. "Os modelos explicativos do transplante de células tronco-hematopoéticas na visão de um grupo de pacientes." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/22/22132/tde-07102009-153550/.
Повний текст джерелаPeople with hematologic disorders face physical, emotional and social alterations. Thus, acting in this area means to be willing to care for the individual, precociously perceiving and intervening in the revealed changes. Hematologic pathologies can be treated by radiotherapy, chemotherapy and hematopoietic stem cell transplantation (HSCT). In most cases, the combination of these types of therapy is needed to control the disease. After undergoing chemotherapy and/or radiotherapy the patient is subject to HSCT, aiming the cure. These treatments are highly aggressive, causing damages in all dimensions of patients lives. Understanding patients sociocultural context is necessary to comprehend the trajectory of someone affected by a disease, since the beginning of the signs and symptoms up to the perspectives for the future, identifying their ideas and behaviors in the fight for survival. In this way, this study aimed to identify the explanatory models (EMs) for allogeneic HSCT of related donors, in the view of a group of patients. In order to achieve this, the medical anthropology, the qualitative case study method and the inductive content analysis technique were established as theoretical bases. Eleven patients participated in the study, three with acute myeloid leukemia, four with chronic myeloid leukemia, two with acute lymphoid leukemia and two with severe aplastic anemia. The six women and five men are adults at reproductive age and their social characteristics show they are from the lower social class. Semi-structured interviews guided by questions that are part of the construction of the explanatory models were carried. Afterwards the interviews were compiled and analyzed in a two-phase data analysis. Interviews were gathered and after several readings, the following thematic categories were listed: from the trajectory of the disease to specialized treatment, the meanings given to the disease and treatments, dealing with the disease and treatments, and life after treatments. Different elements of the explanatory models of becoming ill and undergoing allogeneic HSCT emerged from these categories. The influence of participants cultural context was evidenced. Interviewed subjects reported the signs and symptoms of the disease, the perception of being ill and its causes, the difficult search for health care, home treatments, the need of medications, the existence of a compatible donor, complications and their consequences, the financial difficulties, care and changes in the lifestyle after treatments and the perspectives of future. It is concluded culture is a reference system to people from a social group, which provides ways of thinking and acting about a certain situation or event. Examples of the trajectory followed by the participants in the search for care is evidenced, aiming to motivate health professionals, specially from the nursing team, who delivery care to these patients, to look them in all dimensions of their lives, to provide a comprehensive and differentiated care, integrating cultural knowledge to the biomedical model.
Tanizawa, Roberta Sandra da Silva. "Estudo morfológico e por citogenética da medula óssea de portadores de síndrome mielodisplásica secundária no Serviço de Hematologia do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/5/5167/tde-27092010-145739/.
Повний текст джерелаMyelodysplastic syndromes (MDS) are clonal hematopoietic stem cell disorders, characterized by cytopenias, dysplastic bone marrow (BM) and propensity to progress to acute myeloid leukemia. Secondary MDS are associated with risk factors such as congenital disorders (Fanconis anemia), acquired bone marrow failures, exposure to chemotherapy (alkylating agents, topoisomerase II inhibitors) agents and radiation and chemicals (benzene, petroleum). Immunosuppressive agents associated with hematopoietic growth factors are also associated with secondary MDS. The WHO classification has recently adopted the term therapy-related myeloid neoplasms for cases of myeloid malignancies that fulfill morphological criteria not only for MDS but also for AML or myeloproliferative neoplasms.The aim of the study was to analyze clinical, morphological and cytogenetic features of 42 patients with secondary MDS/MN in a cohort of patients diagnosed at our institution from 1987 to 2008. 23 patients (54.8%) were male, median age 53.5 (4-88) years. 45.2% had primary hematologic malignancies, 26.2% aplastic anemia, 14.3% solid tumors and 14.3% other diseases (autoimmune diseases and solid organ transplantation). 33% had undergone chemotherapy alone, 2% RT alone, 26% both modalities and 28% immunosuppressive agents. Five (11.9%) patients had undergone autologous HSCT for treatment of previous malignancies. The median latency between the primary disease and secondary MDS/MN was 85 (23-221) months. Eight patients underwent allogeneic HSCT (allo- HSCT) for treatment of related secondary MDS. Anemia, neutropenia, thrombocytopenia and peripheral blasts were observed in 64.3%, 54.8%, 78.6% and 26.2%, respectively. BM aspirates was poorly representative in 1/3 of cases, 29.7% global hypocellularity, 62.2% more than 5% of blast counts and 14.3% more than 15% of ring sideroblasts. Dysplasia in erythroid, granulocytic and megakaryocytic series was observed in 79.4%, 77.1% and 68.2%, respectively. Twenty two BM biopsies were performed. Global hypocellularity, ALIP and lymphoid nodules were shown in 9.1%, 23.8% and 40.9%. The immunohistochemistry showed more than 1% of CD34+ and CD117+ cells, clusters of CD34+ and CD117+ and immunoexpression of p53 protein in 77.2%, 82.3%, 59%, 29.4% and 33.3%, respectively. Clonal abnormalities were observed in 84.3% of cases with high prevalence of unbalanced (96%) rearrangements. 37% showed monosomy 7 and 44.4% complex karyotypes. The median overall survival was 5.7 for all patients and 40 months for patients treated with allo-HSCT (P=0.007). Hematologic malignancies, low platelet count, serum high LDH and ferritin, detection of CD117+ clusters, positive immunoexpression of p53, abnormal cytogenetics, intermediate-II or high-risk IPSS groups were associated with poor survival. No parameter studied from bone marrow aspirate had impact in survival. p53 expression was associated to abnormal karyotype (P=0.092) and IPSS risk (P=0.054). There was no association between the presence of ALIP, BM blast counts and immunoexpression of CD34+ and CD117+. Our study shows that cytogenetic analysis and BM immunohistochemistry are very important in diagnosis and prognosis, and that allo-HSCT could improve the survival of secondary MDS/MN. More studies with larger numbers of cases should be conducted to confirm the importance of the IPSS for secondary MDS, probably replacing the bone marrow aspirate blast counts by the immunohistochemistry detection of precursor cells
Xue, Liting. "Oncogene Function in Pre-Leukemia Stage of INV(16) Acute Myeloid Leukemia: A Dissertation." eScholarship@UMMS, 2014. https://escholarship.umassmed.edu/gsbs_diss/740.
Повний текст джерелаXue, Liting. "Oncogene Function in Pre-Leukemia Stage of INV(16) Acute Myeloid Leukemia: A Dissertation." eScholarship@UMMS, 2010. http://escholarship.umassmed.edu/gsbs_diss/740.
Повний текст джерелаDidone, Alline. "Estudo comparativo entre diferentes metodologias na detecção da mutação JAK2V617F em Neoplasias Mieloproliferativas Crônicas BCR-ABL1 negativo." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/5/5167/tde-03022016-143712/.
Повний текст джерелаMyeloproliferative neoplasms (MPN) represent a large group of clonal hematologic malignant diseases with three main members: Polycythemia Vera (PV), Essential Thrombocythemia (ET), and Primary Mielofibroses (PMF). JAK2 is a cytoplasmic tyrosine kinase protein and is important in different signal transduction pathways. Identification of JAK2V617F mutation in PV, ET and PMF is an important advance for understanding the biology of MPN. Differences in the frequency of this mutation are reported among different studies and it is believed that technical sensitivity could be the major reason for this variability. Currently, several techniques for detection of JAK2V617F have been developed, tested and validated for their sensitivity and specificity, including: PCR-RFLP (Restriction Fragment Lenght Polymorphysm), PCR-ARMS (Amplification Refractory Mutation System), PCR-HRM (High-Resolution Melt analysis) and Sanger Direct Sequencing. The present study, evaluated all four molecular diagnostic methods mentioned above blood samples from 136 patients (PV=20; MFP=20; ET=28 and other MPN=68). Comparable results were observed for PV and PMF when all technics were applied. Patients with diagnosis of ET JAK2V617F mutations were detected in 67.8% when PCR-ARMS and PCR-HRM were used whilst PCR-RFLP and direct sequencing detected 71.4% and 64.2% respectively. In 68 patients with suspicion of MPN discordant results were seen between PCR-RFLP (4.4%) and PCR-HRM (1.5%) when compared to PCR-ARMS (3%) and direct sequencing (3%) related to JAK2V617F frequency. In conclusion PCR-ARMS was considered the most reliable methodology for JAK2V617F detection by presenting the lowest risk for cross contamination, less laborious, and the ability in determining allele burden that is becoming an important tool for risk stratification
Chien-LiangLin and 林建良. "The Impact of Inflammatory Bowel Disease Severity and Medication on Subsequent Specific Hematologic Neoplasm." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/90649406312162047363.
Повний текст джерелаChawdhary, Ayesha Bartholomew L. Kay Sharma Shreela. "Delays in diagnosis and referral and treatment for hematologic malignancies." 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1467396.
Повний текст джерелаMartin, Holly René. "Mechanism of Transformation and Therapeutic Targets for Hematological Neoplasms Harboring Oncogenic KIT Mutation." Thesis, 2014. http://hdl.handle.net/1805/5503.
Повний текст джерелаGain-of-function mutations in the KIT receptor tyrosine kinase have been associated with highly malignant human neoplasms. In particular, an acquired somatic mutation at codon 816 in the second catalytic domain of KIT involving an aspartic acid to valine substitution is found in patients with systemic mastocytosis (SM) and acute myeloid leukemia (AML). The presence of this mutation in SM and AML is associated with poor prognosis and overall survival. This mutation changes the conformation of the KIT receptor resulting in altered substrate recognition and constitutive tyrosine autophosphorylation leading to constitutive ligand independent growth. As there are currently no efficacious therapeutic agents against this mutation, this study sought to define novel therapeutic targets that contribute to aberrant signaling downstream from KITD816V that promote transformation of primary hematopoietic stem/progenitor cells in diseases such as AML and SM. This study shows that oncogenic KITD814V (murine homolog) induced myeloproliferative neoplasms (MPN) occurs in the absence of ligand stimulation, and that intracellular tyrosines are important for KITD814V-induced MPN. Among the seven intracellular tyrosines examined, tyrosine 719 alone has a unique role in regulating KITD814V-induced proliferation and survival. Residue tyrosine 719 is vital for activation of the regulatory subunit of phosphatidylinositol 3-kinase (PI3K), p85α, downstream from KITD814V. Downstream effectors of the PI3K signaling pathway, in of leukemic cells bearing KITD814V with an allosteric inhibitor of Pak or its genetic inactivation results in growth repression due to enhanced apoptosis. To assess the role of Rac GEFs in KITD814V induced transformation, EHop-016, an inhibitor of Rac, was used to specifically target Vav1, and found to be a potent inhibitor of human and murine leukemic cell growth. In vivo, the inhibition of Vav or Rac or Pak delayed the onset of MPN and rescued the associated pathology in mice. These studies provide insight on mechanisms and potential novel therapeutic targets for hematological malignancies harboring an oncogenic KIT mutation.
Cristina, Sérgio Manuel Ferreira 1992. "Somatic mutations in philadelphia chromosome-negative myeloproliferative neoplasms." Master's thesis, 2016. http://hdl.handle.net/10451/29530.
Повний текст джерелаMyeloproliferative neoplasms (MPN) include polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF). MPN are characterized by clonal proliferation of myeloid progenitors leading to erythrocytosis, thrombocytosis and/or leukocytosis, and risk of hemorrhagic and thrombotic events, as well as myelofibrosis and blast transformation. The discovery of somatic mutations in MPN, namely JAK2 V617F, JAK2 exon 12, MPL, and CALR mutations, has permitted a more specific approach to diagnosis and treatment. The prevalence of JAK2 V617F mutations is higher than 95% in PV, 50-75% in ET and 40-75% in PMF. JAK2 exon 12 mutations are specific of PV. 20-30% of ET and PMF patients present a CALR mutation. The screening of mutations strengthens the diagnosis of MPN since 97% of MPN have at least one somatic mutation. Interestingly, different mutations grant different phenotype and prognosis. Of particular importance, CALR mutations grant a favorable prognosis in ET and PMF, while ASXL1 mutations confer a poorer outcome. In fact, the use of CALR/ASXL1 status for the prognostication of patients has increased clinical value and was suggested for guidance of therapy in PMF. The increasing importance of mutations in the management of MPN warrants a revision of current diagnostic criteria and prognostic models.
As neoplasias mieloproliferativas (MPN) policitémia vera (PV), trombocitémia essencial (ET) e mielofibrose primária (PMF) são caracterizadas por proliferação clonal de progenitores mielóides, levando a eritrocitose, trombocitose e/ou leucocitose, risco de eventos hemorrágicos e trombóticos, mielofibrose e transformação blástica. A descoberta de mutações somáticas, nomeadamente JAK2 V617F, JAK2 exão 12, MPL e CALR, tem permitido um diagnóstico e tratamento mais específico. A prevalência da JAK2 V617F na PV ultrapassa os 95%, enquanto na ET é de 50-75% e na PMF de 40-75%. As mutações JAK2 exão 12 são específicas da PV. 20-30% dos doentes com ET ou PMF apresentam mutação na CALR. A identificação de mutações reforça o diagnóstico pois 97% das MPN apresentam pelo menos uma mutação. É interessante notar que diferentes mutações conferem diferentes fenótipos e prognósticos. De particular relevo, as mutações da CALR conferem bom prognóstico na ET e na PMF, ao passo que mutações da ASXL1 atribuem piores desfechos. De facto, a utilização do estado da CALR/ASXL1 para a definição do prognóstico na PMF tem valor clínico e foi sugerida com ferramenta para guiar a terapêutica. A importância crescente das mutações na gestão das MPN implica uma revisão dos atuais critérios de diagnóstico e modelos de prognóstico.
Pillay, Dashini. "Diagnosis of haematological malignancies in the era of total laboratory automation: comparison of the Advia 2120 to immunophenotyping and morphology." Thesis, 2015. http://hdl.handle.net/10539/18514.
Повний текст джерелаThe incidence of leukaemia in South Africa is 2.5 per 100 000 and has increased due to HIV. Accurate and timeous diagnosis of leukaemia directly impacts success of patient treatment and consequent survival. Usually the Full Blood count (FBC), white blood cell (WBC) differential count and review of the peripheral blood smear alerts the clinician to the possibility of leukaemia. However the number of qualified and skilled technologists in peripheral and central laboratories is on a continual decline making the performance of the critical function of peripheral blood review a challenge. The Advia 2120 haematology analyser performs a WBC and differential count using principles of flow cytometry and the cytograms generated can be used to classify haematological malignancies through the Peroxidase and nuclear density analysis (PANDA) classification system. The presence of myeloperoxidase (MPO) activity in 3% or more leukaemic blasts confirms acute myeloid leukaemia, and enzyme activity can be detected by immunophenotypic analysis or conventional cytochemistry . Research on the comparison of the Advia 2120 and manual morphologic assessment in the classification of leukaemias is limited in the South African setting, where leukaemia often coincides with infection. The aim of this study was to determine if the FBC, differential count and cytogram assessment by the Advia 2120 using the PANDA classification is as reliable as morphologic assessment in the initial classification of haematological malignancies from peripheral blood samples when using flow cytometry as the gold standard.. 150 cases of confirmed leukaemia were collected. The diagnosis obtained from either PANDA analysis and/or morphological assessment was compared to the diagnosis obtained by immunophenotypic analysis. Secondly, the MPO activity obtained by the Advia peroxidase cytogram was compared to the MPO obtained by conventional methods of immunophenotypic analysis and/or cytochemistry. Using the PANDA analysis system, only 48% (72/150) of cases overall were accurately classified. The inaccuracy was 9.3% (14/150) and 42.7% of cases could not be classified. The positive predictive value (PPV) was 88%. The most significant finding was all of the acute Page | iv promyelocytic leukaemia (APL) cases (8/8) had a distinct pattern and were accurately classified on cytogram analysis alone. Accurate sub-classification of other types of acute myeloid leukaemia using PANDA analysis alone was inconsistent. However, the accuracy in classifying leukaemia was improved when the Advia cytogram was used in conjunction with morphological analysis, as 90% (135/150) of cases were accurately classified. The sensitivity and specificity of the peroxidase cytogram in evaluating myeloperoxidase (MPO) activity was 85% and 88.6% respectively. The agreement between cytogram peroxidase activity and the reference methods was 89.1% and the Cohen’s kappa was 76.9%. To the best of our knowledge, there is no data comparing peroxidase activity on the cytogram to other methods. In conclusion, it was shown that the routine use of the Advia cytograms in conjunction with the morphology findings provides invaluable information in the initial screening of leukaemia. In cases with indistinct morphology, the cytograms have the potential to aid in a provisional classification. The peroxidase activity from the cytogram could be used as a surrogate marker for myeloperoxidase activity in leukaemia. Moreover, a tentative diagnosis of an APL is possible by simple analysis of the cytogram resulting in earlier diagnosis which could be life-saving.
Hanmod, Santosh S. Hewett-Emmett David Peters Ronald J. Chemaly Roy F. "The burden of parainfluenza virus infection in patients with hematological malignancy and hematopoietic stem cell transplant (HSCT) recipients in the absence of active immunization and approved therapy : the role of infection control." 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1470186.
Повний текст джерелаParker, Edward. "Molecular Characterization of a Recurrent t(2;7) Translocation Linking CDK6 to the IGK Locus in Chronic B-cell Neoplasia." Thesis, 2012. http://hdl.handle.net/1807/35520.
Повний текст джерелаFerreira, Daniela Filipa Cardoso. "Relevância do painel de diagnóstico fenotípico de Doenças Linfoproliferativas Crónicas de células B na identificação de rasgos fenotípicos displásicos nas linhas a neutrófilo, monocítica e eritroide." Master's thesis, 2017. http://hdl.handle.net/10316/83090.
Повний текст джерелаNas últimas décadas, tem sido cada vez mais reportado na literatura a presença de doenças múltiplas num mesmo indivíduo, nomeadamente a coexistência de mais do que uma Doença Linfoproliferativa Crónica de células B (DLPC-B), a presença simultânea de uma Gamapatia Monoclonal e/ou Mieloma Múltiplo com Síndrome Mielodisplásica (SMD) e, mais recentemente casos diagnosticados com DLPC-B concomitante a SMD. Contudo, relativamente às últimas duas doenças, não se sabe ao certo qual o impacto da presença simultânea destas duas entidades na sobrevida global do doente, nem de que modo poderá influenciar o valor prognóstico nestes casos.Neste trabalho, avaliou-se a capacidade de um painel de diagnóstico fenotípico construído de forma a identificar e caracterizar fenotipicamente as células B patológicas numa amostra, de forma a poder classificar a doença de acordo com a classificação da OMS, e conseguir concomitantemente identificar rasgos fenotípicos displásicos nas linhas a neutrófilo (avaliação da expressão de CD10, CD43, CD31, CD305, CD11c e CD95), monocítica (CD43, CD31, CD305, CD11c, CD81, CD95, CD49d, CD39 e HLA-DR); e eritroide (CD43, CD95 e CD49d).Foram avaliadas 75 amostras de aspirados medulares de indivíduos, cuja idade variava entre 47 e 93 anos (74,5 ± 10,1 anos), sendo 48 do sexo masculino e 27 do sexo feminino; referentes a 51 amostras de doentes com diagnóstico de DLPC-B, 10 amostras de linfocitose B monoclonal (MBL), 8 amostras de doentes com diagnóstico de SMD e 6 amostras de MO normais. O grupo DLPC-B foi dividido em doentes diagnosticados com Linfomas não Hodgkin-B e em casos diagnosticados com Leucemia Linfocítica Crónica de células B/ Linfoma Linfocítico. Posteriormente, estes foram divididos em casos com displasia morfológica > a 10% das células pertencentes a uma das linhas hematopoiéticas; casos com displasia morfológica < a 10%; casos sem qualquer evidência de displasia morfológica. Em todas estas amostras procedeu-se à caracterização fenotípica das células B, por citometria de fluxo, com base no painel de diagnóstico preconizado pelo consórcio EuroFlow. Numa primeira abordagem comparou-se a expressão dos receptores acima mencionados entre o grupo SMD e Controlo, tendo-se observado diferenças estatisticamente significativas para CD10, CD305 e CD95 na linha a neutrófilo; para CD39 e HLA-DR na linha monocítica e para CD43, CD49d e CD95 na linha eritroide. Fomos depois avaliar se a expressão destes receptores se encontrava alterada nos diferentes grupos de DLPC-B/MBL gerados, tendo-se observado diferenças estatisticamente significativas no grupo com mais de 10% de displasia, para CD10 na linha a neutrófilo, para CD39 e HLA-DR na linha monocítica e para CD43 na linha eritroide. Os resultados obtidos apontam para a utilidade do painel de diagnóstico de DLPC-B na identificação de rasgos fenotípicos displásicos nas linhas hematopoiéticas estudadas, permitindo suspeitar da presença de SMD concomitante com uma DLPC-B. Este estudo permitiu, também, identificar novos receptores expressos nas diferentes linhas estudadas, ainda não descritos na literatura como sendo de interesse para o diagnóstico de SMD.
In the last decades, the presence of multiple diseases in the same individual, such as the coexistence of more than one B-cell Chronic Lymphoproliferative Disorder (B-CLPD), the simultaneous presence of Monoclonal Gamapathy and/or Multiple Myeloma with Myelodysplastic Syndrome (MDS), and more recently cases diagnosed with B-CLPD concomitant with MDS. However, for the latter two diseases, the impact of the simultaneous presence of these two entities on overall patient survival is not known, nether how the prognostic value can be influenced in these cases. In this work, the ability of a panel of phenotypic diagnosis constructed to identify and characterize phenotypically the pathological B cells in a sample, in order to be able to classify the disease according to WHO classification, concomitantly to identify phenotypic dysplastic features in the neutrophil (evaluation of the expression CD10, CD43, CD31, CD305, CD11c and CD95), monocytic (CD43, CD31, CD31, CD11c, CD81, CD95, CD49d, CD39 and HLA-DR) and erythroid lineage (CD43, CD95 and CD49d), Seventy-five samples of spinal aspirates were evaluated, ranging in age from 47 to 93 years (74.5 ± 10.1 years), of which 48 were male and 27 were female; 51 samples from patients diagnosed with B-CLPD, 10 samples from monoclonal B-cell lymphocytosis (MBL), 8 samples from patients diagnosed with MDS and 6 samples from normal bone marrow (BM). The B-CLPD group was divided into patients diagnosed with B-cell Non-Hodgkin's Lymphoma and in cases diagnosed with B-cell Chronic Lymphocytic Leukemia / Lymphocytic Lymphoma. Subsequently, these were divided into cases with morphological dysplasia >10% of the cells belonging to one of the hematopoietic lineages; Cases with morphological dysplasia <10%; Cases without any evidence of morphological dysplasia. In all these samples the phenotypic characterization of B cells was performed by flow cytometry, based on the diagnostic panel recommended by the EuroFlow consortium. In a first approach the expression of the above mentioned receptors was compared between the MDS and Control groups, with statistically significant differences being observed for CD10, CD305 and CD95 in the neutrophil lineage; For CD39 and HLA-DR in the monocytic lineage, and for CD43, CD49d and CD95 in the erythroid lineage. Then, we evaluated whether the expression of these receptors was altered in the different B-CLPD/ MBL groups, and statistically significant differences were observed in the group with more than 10% dysplasia, for CD10 in the neutrophil lineage, for CD39 and HLA -DR in the monocytic lineage and for CD43 in the erythroid lineage. The results obtained point to the usefulness of the B-CLPD diagnostic panel in the identification of dysplastic phenotypic features in the hematopoietic lineages studied, allowing to suspect the presence of concomitant MDS with a B-CLPD. This study also allowed to identify new receptors expressed in the different lineages studied, not yet described in the literature as being of interest for the diagnosis of MDS.