Готові списки джерел за темами / HCT 116 Carcinoma

Добірка наукової літератури з теми "HCT 116 Carcinoma"

Автор: Grafiati

Опубліковано: 6 вересня 2023

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Статті в журналах з теми "HCT 116 Carcinoma"

Apaydin Yildirim Betul. "Anti-cancer, antiproliferative activity of active anionic H2O8 oxygen solution on HCT-116 cancer cell." World Journal of Advanced Research and Reviews 12, no. 2 (November 30, 2021): 179–84. http://dx.doi.org/10.30574/wjarr.2021.12.2.0560.

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HCT116 cells are adherent epithelial cells derived from the human colorectal carcinoma cell line commonly used to study inflammatory responses in colonic epithelial cells. In this study, it was aimed to evaluate the effects of active anionic H2O8 oxygen solution, which is a very strong antiviral and antimicrobial agent, on HCT-116 human colorectal cancer cell line. Cell viability was determined by MTT analysis. Antiproliferative activity of the anionic H2O8 was investigated on HCT 116 (human colorectal carcinoma) cancer cells. Anionic H2O8 displayed the outstanding activities for MTT test, IC50= 9.44 for 24th hour was calculated as IC50= 11.73 for 48th hour on HCT 116 cell line. It is thought that it can serve as an agent with strong potential to be used in treatment.

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Leong, Lek Mun, Kok Meng Chan, Asmah Hamid, Jalifah Latip, and Nor Fadilah Rajab. "Herbal Formulation C168 Attenuates Proliferation and Induces Apoptosis in HCT 116 Human Colorectal Carcinoma Cells: Role of Oxidative Stress and DNA Damage." Evidence-Based Complementary and Alternative Medicine 2016 (2016): 1–12. http://dx.doi.org/10.1155/2016/2091085.

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The use of herbal formulations has gained scientific interest, particularly in cancer treatment. In this study, the herbal formulation of interest, denoted as C168, is a mixture of eight genera of plants. This study aims to investigate the antiproliferative effect of C168 methanol extract (CME) on various cancer cells and its underlying mechanism of action on the most responsive cell line, namely, HCT 116 cells. CME exerted antiproliferative activities on HCT 116 colorectal carcinoma cells and HepG2 hepatocellular carcinoma cells but not on CCD-841-CoN normal colon epithelial cells, Jurkat E6.1 lymphoblastic leukemic cells, and V79-4 Chinese hamster lung fibroblasts. Further investigation on HCT 116 cells showed that CME induced G2/M cell-cycle arrest and apoptosis. Treatment of CME induced oxidative stress in HCT 116 cells by increasing the superoxide anion level and decreasing the intracellular glutathione. CME also increased tail moment value and H2AX phosphorylation in HCT 116 cells, suggesting DNA damage as an early signal of CME induced apoptosis. Loss of mitochondrial membrane potential in CME-treated cells also indicated the involvement of mitochondria in CME induced apoptosis. This study indicated the selectivity of CME toward colon cancer cells with the involvement of oxidative damage as its possible mechanism of action.

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Leischner, Christian, Markus Burkard, Anja Michel, Susanne Berchtold, Heike Niessner, Luigi Marongiu, Christian Busch, Jan Frank, Ulrich M. Lauer, and Sascha Venturelli. "Comparative Analysis of the Antitumor Activity of Cis- and Trans-Resveratrol in Human Cancer Cells with Different p53 Status." Molecules 26, no. 18 (September 14, 2021): 5586. http://dx.doi.org/10.3390/molecules26185586.

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Resveratrol, a natural plant phytoalexin, is produced in response to fungal infection or− UV irradiation. It exists as an isomeric pair with cis- and trans-conformation. Whereas multiple physiological effects of the trans-form, including a pronounced anti-tumoral activity, are nowadays elucidated, much less knowledge exists concerning the cis-isomer. In our work, we analyzed the antiproliferative and cytotoxic properties of cis-resveratrol in four different human tumor entities in direct comparison to trans-resveratrol. We used human cell lines as tumor models for hepatocellular carcinoma (HCC; HepG2, Hep3B), colon carcinoma (HCT-116, HCT-116/p53(−/−)), pancreatic carcinoma (Capan-2, MiaPaCa-2), and renal cell carcinoma (A498, SN12C). Increased cytotoxicity in all investigated tumor cells was observed for the trans-isomer. To verify possible effects of the tumor suppressor p53 on resveratrol-induced cell death, we used wild type and p53-deleted or -mutated cell lines for every tested tumor entity. Applying viability and cytotoxicity assays, we demonstrated a differential, dose-dependent sensitivity towards cis- or trans-resveratrol among the respective tumor types.

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Chandrani, Jeenkal P., and Kalpesh J. Ganatra. "An Efficient And Catalytically Free Chemical Transformation of Pyrimidin-2(1H)-one to 2-(N-Arylamino)pyrimidines and their in vitro Cytotoxicity Evaluation." Asian Journal of Organic & Medicinal Chemistry 5, no. 2 (2020): 133–37. http://dx.doi.org/10.14233/ajomc.2020.ajomc-p260.

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With the aim to develop an efficient strategy to synthesize pyrimidine derivatives bearing diversely substituted amines involves four step linear protocols started with Biginelli multi-component reaction leading to dihydropyrimidines which passing throug multistep sequantial process containing oxidation, chlorination and catalytically free transformation of pyrimidin-2(1H)-one to 2-(N-arylamino)pyrimidines, were evaluated for cytotoxicity study against human cancer lines HCT-116, Hep-G2 and QG-56. Compound 4j exhibit significant anticancer activity showed against: human hepato carcinoma (Hep-G2) and human colon carcinoma (HCT-116) serve as a excellent lead molecule for the generation of various promising targets.

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Tan, Maria Carmens, Glenn G. Oyong, Chien Chang Shen, and Consolacion Y. Ragasa. "CYTOTOXIC LABDANE DITERPENOIDS FROM ANDROGRAPHIS PANICULATA (BURM.F.) NEES." Asian Journal of Pharmaceutical and Clinical Research 10, no. 12 (December 1, 2017): 99. http://dx.doi.org/10.22159/ajpcr.2017.v10i12.19194.

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Objective: The primary objective of this study was to probe the cytotoxic capacity of the labdane diterpenoids andrographolide (1), 14-deoxyandrographolide (2), 14-deoxy-12-hydroxyandrographolide (3), and neoandrographolide (4) on mutant and wild-type immortalized cell lines.Methods: Breast adenocarcinoma (MCF-7), colon carcinomas (HCT-116 and HT-29), small cell lung carcinoma (H69PR), human acute monocytic leukemia (THP-1), and wild-type primary normal human dermal fibroblasts - neonatal cells (HDFn) were incubated with 1-4, and the degree of cytotoxicity was analyzed by employing the in vitro PrestoBlue® cell viability assay. Working solutions of 1-4 were prepared in complete cell culture medium to a final non-toxic dimethyl sulfoxide concentration of 0.2%. The plates were incubated at 37°C with 5% CO2 in a 98% humidified incubator throughout the assay. Nonlinear regression and statistical analyses were done to extrapolate the half maximal inhibitory concentration 50% (IC50). One-way ANOVA (p<0.05) and multiple comparison, Tukey’s post hoc test (p<0.05), were used to compare different pairs of data sets. Results were considered statistically significant at p<0.05.Results: The highest cytotoxicity index was exhibited by the H69PR and 1 trials which displayed the lowest IC50 value of 3.66 μg/mL, followed by HT-29 treated with 2, HCT-116 and 1 trials, and H69PR treated with 4 (IC50=3.81, 3.82, and 4.19 μg/mL, respectively). Only 1 and 4 were detrimental toward MCF-7, while 1, 3, and 4 were degenerative against H69PR. Tukey’s post hoc multiple comparison indicated no significant difference in the cytotoxicity of 1-4 on HCT-116 cells which afforded IC50 values ranging from 3.82 to 5.12 μg/mL. Evaluation of the two colon carcinoma cell lines showed that HCT-116 was categorically more susceptible to cellular damage caused by treatments with 1-4 than was HT-29. Cytotoxicity was not detected in THP-1 and HDFn cells (IC50>100 μg/mL).Conclusion: Diterpenoids 1-4 isolated from the dichloromethane extract of the leaves of A. paniculata exhibited different cytotoxic activities against MCF-7, HCT-116, HT-29, and H69PR. All constituents had comparable action on HCT-116 cells but were not found to be cytotoxic to normal HDFn cells and mutant THP-1 cells.

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Budu, Oana, Christian Dragos Banciu, Codruta Soica, Daniel Florin Lighezan, Andreea Milan, Alexandra Prodea, Alexandra Mioc, Marius Mioc, Gabriel Mardale, and Laurentiu Sima. "Lacticaseibacillus rhamnosus—A Promising Tool for Colorectal Cancer Treatment." Processes 11, no. 3 (March 6, 2023): 781. http://dx.doi.org/10.3390/pr11030781.

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Probiotic strains such as Lactobacillus spp. are already known for their beneficial effect on human health and new research supports their role in colon cancer prevention and treatment. The current study reports the effect of different concentrations of Lacticaseibacillus rhamnosus (LGG, 106–109 CFU/mL), alone or in association with 5-fluorouracil (5-FU, 10 μM), tested against normal HaCaT cells, HT-29 colorectal adenocarcinoma and HCT-116 colorectal carcinoma cell lines. The underlying cytotoxic effect was further investigated. LGG treatment of HT-29 and HCT-116 cells caused a variety of apoptotic-related nuclear morphological changes, as revealed by DAPI staining. ELISA studies showed that LGG treatment increased caspase-3 activity and pro-apoptotic BAX protein levels while decreasing anti-apoptotic Bcl-2 protein levels and the proto-oncogene Cyclin D1. A more detailed examination of the mitochondrial function revealed that high concentrations of LGG can impair mitochondrial function in HT-29 and HCT-116 cancer cells. All of these findings suggest that LGG has a pro-apoptotic, mitochondrial-targeted, cytotoxic effect on both colon cancer cell lines studied.

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Vaziri, S. A., A. Al-Hazzouri, D. R. Grabowski, M. K. Ganapathi, R. M. Bukowski, and R. Ganapathi. "Sorafenib treatment of clear-cell renal cell carcinoma (CCRCC) and colorectal carcinoma (CRC) cells: Differential effects on gene expression and cell death pathways." Journal of Clinical Oncology 25, no. 18_suppl (June 20, 2007): 15612. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.15612.

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15612 Background: The von Hippel Lindau gene (VHL) is often mutated in CCRCC and leads to loss of VHL protein (pVHL) expression. Sorafenib is a TKI with clinical activity in metastatic CCRCC. Studies to define mechanisms governing anti-tumor activity of this agent in CCRCC or CRC cell lines that express wild-type pVHL were conducted. Methods: We evaluated CAKI-1 (CCRCC) and HCT- 116/p53 +/+ (CRC) cell lines as model systems expressing wild-type pVHL. Cells were treated at 37°C in an atmosphere of normoxia (21% O2) or hypoxia (1% O2), 5% CO2 and the remainder N2 in the absence (control) or presence of sorafenib (2.5–20 μM) for 24–96 hours. Expression of target angiogenesis, apoptotic and anti-apoptotic genes was determined by real-time RT- PCR. Fluorescence microscopy following staining with Hoechst 33342 plus propidium iodide was used to analyze cell death by apoptosis and/or necrosis. Caspase-3 activity was measured using the target substrate DEVD-AFC. Results: In CAKI-1 and HCT-116 cells, exposure to 1% O2 relative to 21% O2, led to increased expression (2 to 6-fold) of angiogenesis (VEGF) and anti-apoptosis (TNFAIP3 & MCF2) genes. However, in an atmosphere of 1% O2 relative to 21% O2, a decreased (>2-fold) and increased (>3-fold) expression of the apoptotic (TNFRSF25) gene was observed in CAKI-1 cells and HCT-116 cells. Sorafenib treatment (7.5 μM) of CAKI-1 cells in 1% O2 led to a >3–4-fold decrease in expression of the VEGF and TNFAIP3 and a 3-fold increase TNFRSF25 genes. Following treatment with 10 μM sorafenib for 48h, cell death was >80% by necrosis in CAKI-1 cells and >95% by apoptosis in HCT-116 cells. Apoptotic cell death in the HCT-116 was also confirmed by increased caspase-3 activity in cell extracts following sorafenib treatment. Apoptotic cell death or necrotic cell death induced by sorafenib was unaffected by normoxia or hypoxia. Conclusions: In contrast to CCRCC cells, hypoxia led to upregulation of the apoptotic gene TNFRSF25 in the CRC cells. Anti- proliferative effects of sorafenib were primarily by necrosis in CCRCC cells and by apoptosis in CRC cells. No significant financial relationships to disclose.

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Khulud M Alshehr and Madeha O I Ghobashy. "Antitumor, Antimicrobial activities and Phytochemicals Constituent of different Extracts of Pulicaria undulata (Forssk.) Oliver. Grown Naturally in Saudi Arabia." International Journal of Research in Pharmaceutical Sciences 11, no. 3 (August 11, 2020): 4889–901. http://dx.doi.org/10.26452/ijrps.v11i3.2790.

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Antitumor and antimicrobial resistance are a habitual global issue, which continually demands finding new natural compounds to encounter the resistance. Pulicaria undulata (Forssk.) Oliver. (Asteraceae family) has numerous promising medicinal properties. The recent work aimed at determination of antitumor effects of three extracts of P. undulata on three types of human carcinoma; HEPG-2 hepatocellular carcinoma, MCF-7 breast carcinoma and HCT-116 colon carcinoma cell lines. Anticancer activity was assessed through studying the viability of the cancer cells and apoptotic pathway. Also, antimicrobial potency of different extracts was assessed against studied human pathogens (five Gram negative bacteria, two Gram positive bacteria and yeast). The results reveal that chloroform extract has different levels of cytotoxicity toward the three types of cancer cell lines. A considerable decline in cancer cell rates has been linked to increasing in concentration of plant extract. The half maximal inhibitory concentration IC 50 value was 3.01 µg/ mL for the HepG-2, 16.4 µg/mL for the MCF-7, and 7.4 µg/ mL for HCT-116. Followed by the ethyl acetate extract which showed strong cytotoxic activity against HEPG2 with IC 50 = 12.2 µg ∕ml and moderate activities against MCF7 and HCT 116 and recorded (IC 50 = 26.7 and 26.4 µg ∕ml, respectively). While the crude methanol extract recorded the lowest cytotoxic effect against HEPG2, MCF7 and HCT 116 with (IC 50 = 51.4, 105.1 and 86.7 µg ∕ ml, respectively). Chloroform and ethyl acetate extracts have a high antimicrobial activity more than methanol extract against the pathogens being studied. HPLC and GCMs Analysis identified numerous chemical compounds of P. undulata extracts with various therapeutic benefits. In conclusion, P. undulata has the potential to act as an antimicrobial agent against various pathogenic microbes and is a promising wild herb for the treatment of cancer.

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Kanti Das, Sabuj, Snehasis Mishra, Krishnendu Manna, Utpal Kayal, Supratim Mahapatra, Krishna Das Saha, Sasanka Dalapati, G. P. Das, Amany A. Mostafa та Asim Bhaumik. "A new triazine based π-conjugated mesoporous 2D covalent organic framework: itsin vitroanticancer activities". Chemical Communications 54, № 81 (2018): 11475–78. http://dx.doi.org/10.1039/c8cc07289b.

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Jacinto, Sonia D., Eunice Alexis C. Chun, Anthony Sebastian Montuno, Chien-Chang Shen, Dinah L. Espineli, and Consolacion Y. Ragasa. "Cytotoxic Cardenolide and Sterols from Calotropis Gigantea." Natural Product Communications 6, no. 6 (June 2011): 1934578X1100600. http://dx.doi.org/10.1177/1934578x1100600614.

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The dichloromethane extract from the leaves of Calotropis gigantea Linn. was strongly cytotoxic against non-small cell lung carcinoma (A549), colon carcinoma (HCT 116) and hepatocellular carcinoma (Hep G2), and non toxic to Chinese hamster ovary (AA8). The extract afforded uscharin (1), 3,5,8-trihydroxy-24-methylcholest-6,22-diene (2), a mixture of (24R)-3β-hydroxy-24-ethylcholest-5-en-7-one (3a) and 6β-hydroxy-24-ethylcholest-4,22-dien-3-one (3b), and another mixture of (24R)-24-ethylcholest-4-en-3-one (4a) and (24S)-24-ethylcholest-4,22-dien-3-one (4b). Cardenolide 1 exhibited extreme toxicity to A549, HCT 116 and Hep G2 with IC50 values of 0.003 μg/mL, 0.013 μg/mL, and 0.018 μg/mL, respectively, while sample 3 exhibited an IC50 of 1.35 μg/mL, 4.46 μg/mL, and 3.83 μg/mL, respectively.

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Більше джерел

Дисертації з теми "HCT 116 Carcinoma"

Kashyap, Srishti. "Isolation, Structure Elucidation and Functional Characterization of a Novel Cytotoxic Secondary Metabolite Phomafuranone, 2-Hydroxy-2, 4-dimethyl-5-[-1-propen-1-yl]-3(2H)-furanone, from Phoma tropica an Endophytic Fungus Isolated from Mappia foetida." Thesis, 2017. http://etd.iisc.ac.in/handle/2005/4135.

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Cancer has become the leading disease-related cause of death in the human population. For example, in the United States, cancer is the second leading cause of death behind cardiovascular disease, and it is projected that cancer will become the leading cause of death in the coming years. The medical treatment of cancer still has many unmet needs. The main curative therapies for cancer, surgery and radiation, are generally only successful if the cancer is found at an early localized stage. Once cancer has progressed to metastatic stage, these therapies are less successful. Hence, chemotherapeutic drugs are used for the treatment of these advanced tumors, particularly in the case of the common epithelial tumors such as lung, colorectal, breast, prostate, and pancreatic cancers. New chemotherapeutic drugs are necessary since most of the cancers acquire resistance towards existing anticancer drugs. Nature has always been an attractive source of new chemotherapeutic agents, as a tremendous chemical diversity is found in millions of species of plants, animals, and microorganisms.Microorganisms (bacteria, fungi, actinomycetes) serve as readily renewable and inexhaustible source of novel bioactive metabolites.Endophyte, a microorganism that reside in theinternal tissues of living plantswithout causing any immediate overt negative effects, are potential sources of novel natural products for exploitation in medicine. Mappia foetidais distributed in western part of peninsular coastal India from Konkanghatsto northern parts of the Kanara, Nilgiris, Anamalis, and Pullneys hills of India. It is a rich source of alkaloids such as camptothecin, 9-methoxy camptothecin, mappicin,sitosterol and lupeol and other natural products having anticancer and antimicrobial properties. Since, the secondary metabolite production in fungal endophytes is greatly influenced by theircompetitive ecological niche and interaction with host metabolism, Mappia foetida was chosen for endophytic fungal isolation. Sirsi, (North Karnataka, India) because of its rich biodiversity was chosen as a location for collection of the plant samples. The organicsolvent extracts obtained from mycelia and culture filtrates of all the endophytic fungal isolates were screened for their cytotoxic activity against HeLa (human cervical carcinoma) cellline. Organic extracts of 8 fungal isolates exhibited significant cytotoxic activity, among which Phoma tropica(S1/3) culture filtrate extract exhibited most significant cytotoxicactivity againstHeLa cell line with IC50 of 25µg/ml. Dichloromethane extract of the Phoma tropica culture filtrate was subjected to bioassay–guided column chromatographic fractionationwhich resulted in the isolation of purified cytotoxic secondary metabolite. Based on the analysis of variousspectroscopic techniques such as NMR, FTIR, LC-MS/MS, HRMS, CHNOS elemental analysis and X-ray diffraction studies, the purified metabolite was identified as 2-Hydroxy-2,4-dimethyl-5-[-1-propen-1-yl]-3(2H)furanone(Phomafuranone).Phomafuranone exhibited significant cytotoxic activity against various cancer cell lines (HeLa, Jurkat, COLO 205, HT-29, HCT-15, HCT 116, A549, A-431 and OVCAR-3) Further studies were undertaken to elucidate the mechanism of cytotoxicityof the purified metabolite on human cancer cell lines. Phomafuranone contains a conjugated unsaturated α, β, γ, δ carbonyl pharmacophore moiety. Polyunsaturated carbonyl compounds are referred to as “Michael acceptors” and they behave as soft electrophiles. Michael acceptors react with strong biological nucleophiles such as thiols.The reactivity of electrophilic Phomafuranone with thiol containing biomolecules like glutathione, cysteine and N-acetyl cysteine was investigated by spectrophotometric methods. Cell cycle progressionanalyses oftreated HCT 116 (colorectal carcinoma) cell line by flow cytometry revealed that Phomafuranone arrested significant proportion of cells in G2/M phase. HCT 116 cells were arrested specifically in early mitotic phase of cell cycle as indicated by time dependent increase in phospo-histone3 (Ser10) levels and nuclear import of Cyclin B1.On treatment cancer cells with Phomafuranone, time dependent depletion of intracellular reduced glutathione levels was observed. Depletion of reduced glutathione inturn led to elevated intracellular ROS levels.Pre-treatment of HCT 116 cell lines with thiol containing antioxidants like N- acetylcysteine (NAC) and reduced glutathione (GSH) completely abrogated itscytotoxic effect, suggesting Phomafuranone inducedthiol–mediated cytotoxicity. The elevated ROS levels led to mitochondrial membrane depolarization as indicated by cytochrome c release in cytosol from mitochondria in a time dependent manner. Cytochrome c release was followed caspase 9 mediated apoptotic cell death. Thus, our results suggest that Phomafuranone induced redox imbalancemediated apoptosis in HCT 116 cell line by intrinsic pathway.

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Chung, Ming-Hsien, and 鍾明憲. "Positron Emission Tomography-based study in Hepatocellular carcinoma: 18F-FDG and 11C-MET uptake in HCC cell line." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/78026005703905063226.

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碩士
國防醫學院
生物及解剖學研究所
99
Hepatocellular carcinoma (primary liver malignancy or called hepatoma) is the most common type of liver cancer. In Taiwan, the incidence of hepatoma has been estimated to be 27.7 of 100,000 peoples every year. It ranks the first and second cancer-related death in male and female, respectively. The clinical assessment tools for patients with hepatoma include serum alpha-fetoprotein (AFP) check up and several modalities of imaging study. The latter ones consist of sonography, computed tomography (CT), magnetic resonance imaging (MRI) and positron emission tomography (PET). Among which, PET is useful to survey tumor metabolism by using different tracers. In clinical practice 18F-Fluoro-deoxyglucose (FDG) is the most commonly used oncological PET tracer. Most cancer cells can accumulate FDG via membrane glucose transporter and phosphorylation of FDG by enriched hexokinase expression, thus allowing the FDG-avid tumor cells to be detected by PET. However FDG is not a completely satisfied tracer for hepatomas because of the various expression of glucose-6-dephosphatase (G6PD) inherent to hepatocytes, which may evacuate the tracer from tumor cells once FDG is de-phosphorylated by G6PD. The G6PD activity varies in hepatomas with different differentiation and therefore affects the detection rate of FDG PET. Alternative PET tracers such as 11C-acetate and 11C-choline have been proposed and their use to detect certain non-FDG-avid hepatomas has been reported preliminarily. Our previous study has demonstrated the use of 11C-methionine (Met) to localize hepatomas. Briefly we found that the primary hepatomas showed Met-void while extra-hepatic hepatomas presented as Met-avid lesions. Conversely the intra-hepatic Met-void lesion showed FDG avidity. To further explore the related mechanism, the study of in vitro Met uptake in hepatomas was performed using three different cell lines (HepG2, Hep3B, HA59T). Also two known differentiation modifiers including All-trans retinoic acid (ATRA) and Trichostatin A (TSA) were adopted to intervene the hepatomas. Met and FDG uptake in ATRA- or TSA-treated hepatomas were compared and correlated with the pertinent tumor growth. The result showed that all these three cell-lines shared the response of growth arrest as well as increased apoptotic potential with either ATRA or TSA treated hepatoma cells in vitro. Their FDG uptake was significantly lowered and Met uptake was enhanced in presence of TSA. The change followed a dose-depend manner. With ATRA treatment, dose-dependent increased Met uptake was also demonstrated in hepatomas. However there was similar change of FDG uptake in ATRA-treated hepatomas. In conclusion Met uptake in hepatomas can be regulated by ATRA or TSA treatment and whether it is related to the effects of differentiation or anti-tumor activity known for ATRA or TSA remains uncertain. The role of Met uptake as a biomarker for hepatoma therapeutics related research warrants for further study.

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Тези доповідей конференцій з теми "HCT 116 Carcinoma"

Šeklić, Dragana, Milena Jovanović, Nevena Milivojević, and Marko Živanović. "PLATINUM(IV) COMPLEX AND ITS CORRESPONDING LIGAND SUPPRESS CELL MOTILITY AND PROMOTE EXPRESSION OF FRIZZLED-7 RECEPTOR IN COLORECTAL CANCER CELLS." In 1st INTERNATIONAL Conference on Chemo and BioInformatics. Institute for Information Technologies, University of Kragujevac, 2021. http://dx.doi.org/10.46793/iccbi21.288s.

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Suppression of cell movement is an imperative in the effectiveness of future generations of chemotherapeutics. Frizzled 7 receptor (FZD7), as the first protein of Wnt/β-catenin signaling cascade, plays a significant role in regulation of cell differentiation, proliferation, and cell migration. This study aimed to investigate the potential effects of platinum (IV) complex: [PtCl4 (dbu-S, S-eddp)] – C1, and its corresponding ligand – L1 on cell movement, as well as the FZD7 expression and localization after treatments on two human colorectal carcinoma cell lines (HCT-116, SW-480). A Wound healing assay was used to examine cell migration, while FZD7 protein expression was examined by immunofluorescence. Chemical compounds, especially L1, reduced cell motility of both tested cell lines. They showed a particularly good effect on HCT-116 cells, increasing protein expression of the antimigratory marker FZD7 whose localization was observed on the cell membrane of HCT-116 cells. Suppression of cell movement was significantly lower in SW-480 cells after treatments, when compared to HCT-116, with an obvious decrease of FZD7 receptor expression and its localization in the cytoplasm of these cells. Our results indicate that among the examined treatments, the ligand showed more significant results in the suppression of HCT-116 cell movement, most likely through the stimulation of differentiation, which is indicated by the promotion of FZD7 expression.

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Milenković, Dejan A., Marko N. Živanović, Milan S. Dekić, Marijana Stanojević Pirković, and Jelena R. Đorović Jovanović. "CYTOTOXIC ACTIVITY AND MOLECULAR DOCKING STUDY OF 4- SUBSTITUTED FLAVYLIUM SALT." In 1st INTERNATIONAL Conference on Chemo and BioInformatics. Institute for Information Technologies, University of Kragujevac,, 2021. http://dx.doi.org/10.46793/iccbi21.466m.

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In the present manuscript, the cytotoxic activity of flavylium cation substituted at 4- position with phenyl (FC-4Ph) was tested to two cells lines (human colorectal carcinoma, HCT-116, and human fibroblast lung, MRC-5). In vitro cytotoxicity experiments were performed to elucidate the possible anticancer activity of tested substance. Investigated compound did not show cytotoxic effect on HCT-116 after 24 h, while after 72 h exerted significant effect. A significant selectivity towards colorectal carcinoma cells was observed. On the other hand, this compound did not show any effect on MRC-5 cell line. The molecular interactions between receptor tyrosine kinase (RTK) and title compound was examined. The crystal structure of investigated receptor RTK was downloaded from Protein Data Bank. The native bound ligand ((E)-[4-(3,5-difluorophenyl)-3H-pyrrolo[2,3-b]pyridin-3-ylidene](3- methoxyphenyl)methanol was extracted from receptor and binding pocket analysis was performed. Re-docking was carried out with the FC-4Ph in order to generate the same docking pose as found in co-crystallized form of receptor. The obtained results of revealed that investigated compound binds at the same binding pockets to RTK, as well as native bound ligand, by weak non-covalent interactions. The most prominent interactions are hydrogen bonds, π-alkyl, and π-π interactions. The preliminary results suggest that investigated compound showed good binding affinity against RTK, as evident from the free binding energy (ΔGbind in kJ/mol).

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Ekine-Afolabi, Bene Akromaa, Sandra Appiah, Azra Pachenari, and Lucy Ghali. "Abstract LB-271: Inulin inhibits free radical species in HCT 116 adenoma carcinoma and normal human HK (keratinocyte) cell line." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-lb-271.

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Pogorzelska, Aneta, Jarosław Sławiński, and Anna Kawiak. "New N-Benzenesulfonylguanidine Derivatives and Their Selective Growth Inhibition of Human Breast Cancer Cell Line MCF-7 and Colon Carcinoma HCT-116." In ECMC 2022. Basel Switzerland: MDPI, 2022. http://dx.doi.org/10.3390/ecmc2022-13281.

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Lee, Michael J., Robert J. M. Frey, Steven D. Frey, Anthony H. Yeo, Janna M. Vassantachart, Peter S. Han, Sara E. Stump, Ying Ming B. Tan, Randy P. Hausted, and Brian YY Wong. "Abstract LB-21:Taraxacum officinale(common dandelion) inhibits azoxymethane-induced aberrant crypt foci formation in C67BL/6 mice and regulates apoptosis in mice and in human colorectal carcinoma HCT-116 cells." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-lb-21.

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Hotta, Naoki. "IDDF2023-ABS-0005 Hepatocellular carcinoma (HCC) treatment experience using smart fusion in dialysis patients." In Abstracts of the International Digestive Disease Forum (IDDF), Hong Kong, 10–11 June 2023. BMJ Publishing Group Ltd and British Society of Gastroenterology, 2023. http://dx.doi.org/10.1136/gutjnl-2023-iddf.123.

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Xu, Ming Jing, Kit Ho Lai, Shu Hai Lin, Pui Wah Tse, David Kung Chun Chiu, Hui Yu Koh, Cheuk Ting Law, et al. "Abstract 1058: Targeting pentose phosphate pathway (PPP) represents a novel therapeutic strategy for hepatocellular carcinoma (HCC) treatment." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-1058.

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Cheo, Feng-Yi, Kai-Siang Chan, Wei-Xuan Tay, and Vishal Shelat. "IDDF2022-ABS-0193 Outcomes of preoperative transarterial chemoembolization (TACE) compared to upfront liver resection for large (≥5cm) hepatocellular carcinoma (HCC): a systematic review and meta-analysis." In Abstracts of the International Digestive Disease Forum (IDDF), Hong Kong, 2–4 September 2022. BMJ Publishing Group Ltd and British Society of Gastroenterology, 2022. http://dx.doi.org/10.1136/gutjnl-2022-iddf.119.

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El-Khoueiry, Anthony, Mary F. Mulcahy, Tanios Bekaii-Saab, Richard Kim, Crystal Denlinger, Rakesh Goel, Shweta Gupta, et al. "Abstract 2947: Pharmacodynamic (PD) and pharmacokinetic (PK) results of the second-generation hypomethylating agent, SGI-110, in patients with hepatocellular carcinoma (HCC) after progression on sorafenib." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-2947.

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Trochon-joseph, Véronique, Caroline Lemarchand, Vincent Hayes, Yamina Rayah, Jean-Louis Labernardière, and Graham Dixon. "Abstract 2143: Mechanistic study of the relative cytotoxicity of doxorubicin loaded nanoparticle formulation compared to free doxorubicin in hepatocellular carcinoma (HCC) cell lines." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-2143.

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