Дисертації з теми "Halophilic bacterium"
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Choquet, Christian. "Physiological studies of a moderately halophilic bacterium, Vibrio costicola." Thesis, University of Ottawa (Canada), 1990. http://hdl.handle.net/10393/5591.
Повний текст джерелаAston, John. "Response to osmotic stress by the haloalkaliphilic bacterium Halomonas campisalis." Online access for everyone, 2006. http://www.dissertations.wsu.edu/Thesis/Spring2006/j%5Faston%5F031406.pdf.
Повний текст джерелаAlbaugh, Catherine Elizabeth. "Degradation of monoaromatic compounds by an aerobic halotolerant alkaliphilic bacterium." Online access for everyone, 2005. http://www.dissertations.wsu.edu/Thesis/Summer2005/c%5Falbaugh%5F081905.pdf.
Повний текст джерелаThomas, Tatiana. "Étude du potentiel biotechnologique de Halomonas sp. SF2003 : application à la production de polyhydroxyalcanoates (PHA) Complete genome sequence of the halophilic PHA-producing bacterium Halomonas sp. SF2003: insights into its biotechnological potential PHA Production and PHA Synthases of the Halophilic Bacterium Halomonas sp. SF2003." Thesis, Lorient, 2019. http://www.theses.fr/2019LORIS542.
Повний текст джерелаDepletion of oil ressources coupled to pollution caused by over-exploitation of plastics generate a plentiful of issues and lead to an urgent need for alternatives. PolyHydroxyAlkanoates (PHA) are biopolymers which have distinguished and naturally imposed themselves due to their biosourcing and biodegradability features. Their synthesis by a wide variety of eukaryotic and prokaryotic organisms, from various carbon substrates, makes their production almost unlimited and allows obtaining polymers exposing rigid thermoplastic to elastomeric properties. Despite that, the production yield and the cost are still the main locks to their development. Among possibilities studied, exploitation of marine resources, like halophilic bacteria, arouse a strong interest since adaptability of these strains is extensive and attractive. Halomonas sp. SF2003 is a PHA producing marine bacterium which naturally exposes a considerable versatility in front of carbon substrates and environmental conditions. Characterization and optimization of its PHA production are the main subjects of the presented study. Genome sequencing and annotation, in addition to phenotypic tests, allowed characterization of various genes and metabolic pathways attesting of the adaptative strain character. In the same time, impact of various parameters on Halomonas sp. SF2003 PHA production has been investigated. This work, employing molecular biology and bioprocess tools, will contribute to future development of biotechnological potential of Halomonas sp. SF2003
Kadam, Priya. "Physiology of halophilic, methylotrophic methanogens /." Full text open access at:, 1996. http://content.ohsu.edu/u?/etd,652.
Повний текст джерелаRichards, Abigail Marie. "Identification and structural characterization of siderophores produced by halophilic and alkaliphilic bacteria." Online access for everyone, 2007. http://www.dissertations.wsu.edu/Dissertations/Summer2007/A_Richards_072707.pdf.
Повний текст джерелаCruzeiro, Sofia Maria de Albuquerque. "Isolation and characterization of halophilic bacteria from salt pans of Ria de Aveiro." Master's thesis, Universidade de Aveiro, 2018. http://hdl.handle.net/10773/22004.
Повний текст джерелаHalophilic microorganisms and the metabolites involved in their adaptive strategies offer a wide variety of potential biotechnological applications and like other extreme environments, hypersaline habitats represent reservoirs of new bioactive compounds. The main objective of this work was to assess the biotechnological potential of halophilic bacteria from traditional salt pans of Ria de Aveiro in terms of biosurfactant production, plant growth promoting traits, quorum-quenching effect and extracellular enzymatic activity. Water from an active salt pan of Ria de Aveiro was collected in early autumn when the concentration of salt was at the saturation level. Using a culture-dependent approach, 14 bacterial strains were isolated and identified by sequencing of 16S rRNA gene fragments as species of Bacillus, Halobacillus, Idiomarina and Marinobacter. In order to assess the biotechnological potential of these halophiles, they were tested for biosurfactant production, plant growth promoting traits (motility, biocontrol effect and siderophore production), quorum-quenching effect and, activity of extracellular enzymes (phosphatase and esterase). None of the isolates demonstrated significant tensioactive or emulsification effects that could be interpreted as indicative of biosurfactant production. However, all isolates were motile even in presence of 10% NaCl and produced siderophores. All Idiomarina and Marinobacter strains caused growth inhibition of the phytopathogenic fungus Alternaria. Quorum sensing inhibition was also detected in isolates of the 4 genera although with differences between species and, in some cases, depending on the salinity of the culture medium. Esterase and phosphatase activity was detected in all isolates and maximum hydrolysis rates of the model substrates were the highest in Halobacillus species. Although biosurfactant production could not be demonstrated, the halophilic isolates displayed an interesting set of features in terms of plant-growth promoting traits with potential application on the emerging field of saline agriculture.
Os microrganismos halofílicos e os metabolitos envolvidos nas suas estratégias adaptativas apresentam uma ampla gama de potenciais aplicações biotecnológicas e, tal como outros ambientes extremos, os habitats hipersalinos são vistos como um reservatório de novos compostos bioativos. O objetivo deste trabalho foi avaliar o potencial biotecnológico de bactérias halofílicas de salinas da Ria de Aveiro em termos de produção de biossurfactantes, características de promoção do crescimento de plantas, efeito de quorum-quenching e atividade enzimática extracelular. No início do outono de 2016, quando a concentração de sal atinge os valores máximos colheu-se água do tanque de cristalização de uma salina ativa da Ria de Aveiro (Marinha de Santiago da Fonte). Utilizando uma abordagem dependente de cultivo, foram isoladas 14 estirpes bacterianas que foram posteriormente identificadas por sequenciação de fragmentos de genes de rRNA 16S como espécies de Bacillus, Halobacillus, Idiomarina e Marinobacter. Para avaliar o potencial biotecnológico destas halófilas, testou-se a produção de biossurfactantes, características de promoção do crescimento de plantas (mobilidade, efeito de biocontrolo e produção de sideróforos), efeito de quorum-quenching e atividade enzimática extracelular (fosfatase e esterase). Nenhum dos isolados demonstrou efeitos tensioativos ou de emulsificação significativos que pudessem ser interpretados como indicativos da produção de biossurfactantes. No entanto, todos os isolados exibiram mobilidade, mesmo na presença de 10% de NaCl e produziram sideróforos. Todas as estirpes de Idiomarina e Marinobacter causaram inibição do crescimento do fungo fitopatogénico Alternaria. A inibição de quorum sensing também foi detetada em isolados dos 4 géneros, embora com diferenças entre espécies e, em alguns casos, dependendo da salinidade do meio de cultura. A atividade de esterases e da fosfatases foi detetada em todos os isolados e as mais altas taxas de hidrólise dos substratos modelo foram observadas nas espécies de Halobacillus. Embora a produção de biossurfactantes não tenha sido demonstrada, os isolados halofílicos exibem um conjunto de características interessantes em termos de promoção do crescimento de plantas, com potencial aplicação no campo emergente da agricultura salina.
Tregoning, George Seibert. "Isolation and Characterization of Halophilic Heterotrophic Bacteria from Lake Vanda, McMurdo Dry Valleys, Antarctica." OpenSIUC, 2010. https://opensiuc.lib.siu.edu/theses/395.
Повний текст джерелаPaul, Varun. "Electricity generation and ethanol production using iron-reducing, haloalkaliphilic bacteria." Diss., Rolla, Mo. : Missouri University of Science and Technology, 2009. http://scholarsmine.mst.edu/thesis/pdf/Paul_09007dcc8069dfe5.pdf.
Повний текст джерелаVita. The entire thesis text is included in file. Title from title screen of thesis/dissertation PDF file (viewed August 10, 2009) Includes bibliographical references (p. 58-64).
Abosmaha, Mohammed Abdolkader. "Molecular identification and physiological characterization of halophilic and alkaliphilic bacteria belonging to the genus Halomonas." Thesis, University of Sheffield, 2013. http://etheses.whiterose.ac.uk/4647/.
Повний текст джерелаMoyo, Anesu Conrad. "Biological approach to improving the evaporation rates of mine wastewater desalination brine treated in evaporation ponds." University of the Western Cape, 2021. http://hdl.handle.net/11394/7982.
Повний текст джерелаThe disposal of brine effluent from inland wastewater desalination plants is a growing global problem with adverse economic and environmental implications because of the substantial cost associated with its disposal and the potential for polluting groundwater resources. Currently, the best and most economical option for brine disposal from inland desalination plants is the use of evaporation ponds, which concentrate the liquid until getting a solid waste that can be valued or directly managed by an authorized company. The effectiveness of these ponds is therefore dependent on the evaporation rate, which has previously been improved by the addition of dyes such as methylene blue. However, the addition of chemical dyes to the evaporation ponds poses a threat to the environment, wildlife, and humans.
Salman, Dilgimen Aydan [Verfasser]. "Proteomics studies of Halomonas salina and a new moderately halophilic bacteria living in saltern areas / Aydan Salman Dilgimen." Berlin : Freie Universität Berlin, 2008. http://d-nb.info/1023327805/34.
Повний текст джерелаKaye, Jonathan Zachary. "Ecology, phylogeny and physiological adaptations of euryhaline and moderately halophilic bacteria from deep-sea and hydrothermal-vent environments /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/11064.
Повний текст джерелаPiubeli, Francine Amaral. "Caracterização da comunidade microbiana em ambientes salinos e suas possíveis aplicações biotecnológicas." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/254728.
Повний текст джерелаTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
Made available in DSpace on 2018-08-18T08:43:54Z (GMT). No. of bitstreams: 1 Piubeli_FrancineAmaral_D.pdf: 6673876 bytes, checksum: 678469d11c710488c1e19eda0ee7d179 (MD5) Previous issue date: 2011
Resumo: A água residuária da extração de petróleo é altamente salina e contém uma mistura complexa de hidrocarbonetos, muitos dos quais são altamente tóxicos. Com o aumento da preocupação em preservar o meio ambiente, as tecnologias voltadas para a recuperação e despoluição de áreas degradadas tem ganhado a atenção. A biorremediação tem sido utilizada como método de biodegradação natural através da otimização de processos biológicos por ser econômica, versátil e a que mais se aproxima de uma despoluição ecologicamente aceitável. No entanto, a biorremediaçao depende de alguns fatores tais como: pH, temperatura, salinidade e pressão. Condições extremas desses fatores podem matar ou inibir espécies que não estejam adaptadas a ambientes extremos, por isso o desenvolvimento e otimização de processos de biorremediação de ambientes extremos contaminados por é de grande relevância. Desta forma, este trabalho teve como objetivos o isolamento e caracterização de bactérias halofílicas presentes na água residuária da extração de petróleo, além do estudo molecular dos genes envolvidos na degradação de compostos aromáticos para que posteriormente utilizássemos essas linhagens na biorremediação desse efluente, outra vertente desse trabalho foi o estudo da diversidade microbiana presente nesta água para auxiliar o direcionamento de esforços na minimização dos impactos causados com a exploração de petróleo. Foram isoladas sete linhagens de bactérias halofílicas da água de produção de petróleo, sendo todas pertencentes a família Halomoneaceae e com potencial para degradação de compostos aromáticos. A linhagem que apresentou maior taxa de crescimento nos compostos testados (ácido benzóico, fenol, ácido p-hidroxibénzoico) foi a denominada df2. Com relação à taxa de degradação dos compostos aromáticos testados, a dp3 degradou cerca 90% do fenol disponível, seguida pela df2 que degradou 80% desse mesmo composto. Quando a fonte de carbono foi o ácido benzóico, a df1 e DP3 degradaram 99% do composto e 70% do ácido p-hidroxibenzóico foi degradado em 12 dias de experimento pela linhagem df2. Para a degradação desses compostos a linhagem Halomonas organivorans seguiu a via de degradação do catecol na rota do ß- cetoadipato compreendendo a codificaçao de diferentes enzimas por catRBCA. A comparação das seqüências da biblioteca de bactérias com as sequências do gene RNA ribossomal 16S presente nos bancos de dados GenBank e RDP revelou a presença de micro-organismos pertencentes aos gêneros Marinobacter e Halomonas e na comparação da técnica clássica de isolamento com a técnica independente de cultivo (biblioteca do gene RNA ribossomal 16S), foi encontrada uma maior diversidade na última. Finalmente de todos os testes realizados na tentativa de biorremediar a água de produção de petróleo, a bioestimulação foi a mais efetiva, sendo conseguida uma diminuição de 77% da carga orgânica com a adição de solução de fosfato e alanina. Estes resultados demonstraram o grande potencial destas linhagens para a degradação de compostos aromáticos, bem como para a biorremediação da água de produção de petróleo, além de descrever a via metabólica utilizada por membros da família Halomoneaceas na degradação de compostos aromáticos, auxiliando assim nos esforços para biorremediação de ambientes salinos contaminados
Abstract: The wastewater from oil extraction (produced water) is highly saline and contains a complex mixture of hydrocarbons, many of which are highly toxic. With increasing concern about preserving the environment, the technologies for recovery and remediation of degraded areas has been gaining attention. Bioremediation has been used as a method of natural biodegradation by optimizing biological processes because it is economical, versatile and it is the closest to a ecologically acceptable decontamination. However, bioremediation depends on factors such as pH, temperature, salinity and pressure. Extremes of these factors can inhibit or kill species that are adapted to extreme environments, so the development and optimization of bioremediation processes for contaminated extreme environments is of great importance. Thus, this work aimed at the isolation and characterization of halophilic bacteria present in the wastewater from oil extraction, and molecular studies of the genes involved with the degradation of aromatic compounds so that later we could use these strains in the bioremediation of this wastewater. Another aspect of this work was to study the microbial diversity present in this wastewater to assist in directing the efforts to minimize the impact of oil drilling. Seven strains of halophilic bacteria were isolated from of the produced water of oil production, all belonging to the family Halomoneaceae having potential for degradation of aromatic compounds. The strain that showed the highest growth rate in the tested compounds (benzoic acid, phenol, p-hydroxybenzoic acid) was strain DF2. Regarding the rate of degradation of the aromatic compounds tested, dp3 degraded about 90% of available phenol, followed by DF2 that degraded 80% of this compound. When the carbon source was benzoic acid, DP3 and df1 and degraded 99% and strain DF2 degraded 70% when the compound was p-hydroxybenzoic acid. For the degradation of these compounds the strain Halomonas organivorans followed the catechol degradation pathway ending in the formation of ß-Ketoadipate and was shown to produce various enzyme activities encoded by catRBCA. Comparison of the 16S rRNA gene sequences of the isolates with the sequences present in the GenBank and RDP databases revealed the isolates belonged to the genera Marinobacter and Halomonas. Comparing these results with those obtained by classical taxonomic techniques demonstrated that the 16S ribosomal RNA gene analysis (culture independent method), found greater diversity. Finally, of all tests performed to bioremediate the produced water, biostimulation by addition of nutrients was the most effective, and achieved a reduction of 77% of the organic load with the addition of phosphate combined with alanine. These results demonstrate the great potential of these strains for the degradation of aromatic compounds, as well as for the bioremediation of produced water from crude oil production, and describe the metabolic pathway used by members of the family Halomoneaceas for the degradation of aromatic compounds, thus assisting in efforts to develop methods for the bioremediation of contaminated saline environments
Doutorado
Ciência de Alimentos
Doutor em Ciência de Alimentos
Sioud, Mouldy. "Etude des mecanismes moleculaires d'action des inhibiteurs des adn topoisomerases 2 chez les archaebacteries halophiles." Paris 7, 1988. http://www.theses.fr/1988PA077155.
Повний текст джерелаSánchez-Román, Mónica. "Calibration of microbial and geochemical signals related to dolomite formation by moderately halophilic aerobic bacteria: significance and implication of dolomite in the geologic record." Zürich : ETH, 2006. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=16875.
Повний текст джерелаSANCHEZ-LOPEZ, ROSANA. "Partie i : etude des mecanismes impliques dans la resistance au sel chez une bacterie halophile- partie ii : etude des relations structure-fonction de metalloproteinases de la famille collagenase." Université Louis Pasteur (Strasbourg) (1971-2008), 1989. http://www.theses.fr/1989STR13020.
Повний текст джерелаGurgel, Teresa Emanuelle Pinheiro. "Avaliação da qualidade da carne-de-sol produzida e comercializada em municípios do Rio Grande do Norte." Universidade Federal Rural do Semi-Árido, 2010. http://bdtd.ufersa.edu.br:80/tede/handle/tede/347.
Повний текст джерелаIt evaluated the quality of carne-de-sol in the period from June to September of 2010 sold in five cities of Rio Grande do Norte - Mossoró, Apodi, Areia Branca, Baraúna and Grossos. It was collect 80 samples of carne-de-sol, of which 44 were from small establishments and 36 purchased in supermarkets and stores. The samples were subjected to microbiological testing - Salmonella, determining the most probable number of fecal coliform, halophilic bacteria and Staphylococcus aureus, the samples were positive for this bacteria were analyzed for resistance to antibiotics, and physical-chemical properties (pH, water activity, minerals and humidity). There was an interaction between intrinsic pH value (μ = 5.702) and Aa (μ = 0.868), , indicating a favorable environment for their multiplication, resulting in values that stood out significantly in what is expected legislation counting S. aureus (μ = 4.81 log UFC at 78.75%), Salmonella (25%) and fecal coliform (μ = 2.36 LogNMP / g, 63.75%). A significant correlation (p <0.05) between the number of halophilic bacteria (μ = 4.87 log UFC) and S. aureus, such conditions may be used as an indicator of contamination in the sun-dried meat. To the antibiotics, 22.5% of strains were resistant to gentamicin, whereas for penicillin G, chloramphenicol, tetraciclina were 100%, 67.5% and 46.26% respectively. It was found that the meat produced and marketed in the sun studied region is improper (96.25%) for consumption in accordance with Brazilian law
Foram avaliadas a qualidade da carne-de-sol no período de junho a setembro de 2010 comercializada em cinco cidades do Rio Grande do Norte Mossoró, Apodi, Areia Branca, Baraúna e Grossos. Sendo coletadas 80 amostras de carne-de-sol, as quais 44 foram provenientes de estabelecimentos de pequeno porte e 36 adquiridas em supermercados e frigoríficos. As amostras foram submetidas à análises microbiológicas Salmonella, determinação do número mais provável de coliformes termotolerantes, bactérias halofílicas e Staphylococcus aureus, as amostras positivas para esta bactéria foram submetidas à análise de resistência à antibióticos; e físico-químicas (pH, atividade de água, cinzas e umidade). Verificou-se interação dos fatores intrínsecos, pH (µ=5,702) e Aa (µ=0,868), do produto com a contagem bacteriana, indicando ambiente favorável para a sua multiplicação, resultando assim em valores que sobressaíram de maneira significativa o que está previsto na legislação para contagem de S. aureus (µ=4,81LogUFC, em 78,75%), Salmonella (presença em 25%) e coliformes termotolerantes (µ=2,36LogNMP/g, em 63,75%). Houve correlação significativa (p<0,05) entre a contagem de bactérias halofílicas (µ=4,87LogUFC) e a de S. aureus, podendo aquelas serem utilizadas como indicador de contaminação na carne-de-sol. Quanto à sensibilidade das cepas S. aureus aos antibióticos, 22,5% das cepas apresentaram resistência à gentamicina, enquanto para penicilina G, tetraciclina e cloranfenicol foram 100%, 67,5% e 46,26% respectivamente. Ficou constatado que a carne de sol produzida e comercializada na região estudada apresenta-se imprópria (96,25%) para o consumo de acordo com a legislação brasileira
Al, Mallah Maha. "Biodegradation des hydrocarbures dans les milieux sursales." Aix-Marseille 2, 1988. http://www.theses.fr/1988AIX22040.
Повний текст джерелаGuinet, Françoise. "Purification et étude du facteur d'élongation Tu d'Halobacterium marismortui." Grenoble 1, 1987. http://www.theses.fr/1987GRE10163.
Повний текст джерелаR, Hemamalini. "Studies on halophilic marinobacter sp.emb5 lipase : novel characteristics, aggregation and proteomics." Thesis, 2017. http://localhost:8080/iit/handle/2074/7403.
Повний текст джерелаSyu, Yuan-Gang, and 許元綱. "Effects of various cultural conditions on the production of ectoine and hydroxyectoine using the halophilic bacterium Corynebacterium glutamicum." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/cdv62t.
Повний текст джерела元智大學
生物科技與工程研究所
106
Ectoine and Hydroxyectoine are compatible solutes produced by halophilic or halotolerant microorganisms in extremely saline environments to protect cellular activity. Ectoine and Hydroxyectoine have the functions of regulating osmotic pressure balance, protecting cell structure and maintaining enzyme activity. Between them, Hydroxyectoine has an extra -OH group in structure than Ectoine, which can replace water and partial water molecule loss in reaction. This stabilizes the natural structure of biopolymers, thereby enhancing the commercial value of Hydroxyectoine. Therefore, the main purpose of this study was to find a strain with superior performance in Hydroxyectoine production, and to achieve higher Hydroxyectoine production through regulation of culture conditions. Among the many strains that produce Ectoine and Hydroxyectoine, Corynebacterium glutamicum, which prefers to produce Hydroxyectoine, was used as a research object. In this study, we investigate the effect of Ectoine and Hydroxyectoine production by different environmental factors (i.e. rotation speed, temperature, pH and salt concentration) and medium compositions (i.e. carbon source, nitrogen source, iron ion and ketoglutaric acid). The results show that the best Ectoine and Hydroxyectoine production is 0.096 g/L and 2.579 g/L when C. glutamicum were incubated at 200 rpm, 30 °C, pH 9 and 0.5 M salt concentrations. In the screening results of the medium composition, it was found that when the carbon source was selected from yeast extract, the nitrogen source was Ammonium sulfate, and 0.5 mM iron ion and 75 mM ketoglutaric acid were added, the Ectoine yield was increased from 0.096 g/L to 0.1 g/L. Hydroxyectoine production increased from 2.579 g/L to 3.15 g/L. Taking into account the fact that the changes in culture conditions have no significant difference in the production of Hydroxyectoine by C. glutamicum, this study will follow up on different fermentation strategies and expect to significantly increase the production of Hydroxyectoine by C. glutamicum.
Barrau, Clara. "Structural characterisation of endotoxins from marine and halophilic bacteria." Tesi di dottorato, 2018. http://www.fedoa.unina.it/12477/1/barrau_clara_31.pdf.
Повний текст джерелаCheng, Yu-Shan, and 鄭伃珊. "Diversity of Halophilic Gram-positive Bacteria Isolated from Coastal and Estuarine Environments." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/47741221415551238963.
Повний текст джерела國立臺灣大學
海洋研究所
91
Abstract Halophilic Gram-negative bacteria are widely found in coastal zones and marine areas. They are also commonly present in the brackish water environments of estuary. In contrast, halophilic Gram-positive bacteria are rare in the microbial communities of estuary, coast and sea. Our laboratory had totally isolated 113 strains of halophilic or halotolerant bacteria, preliminarily identified as Gram-positive bacteria, from the sediment under mangroves at Tan-shui River estuary and Gau-Mei Taichung, and marine water at the coastal sites of Taiwan (Er-jen River estuary etc.) as well as Ba-dou-Zi Keelung and Shen-Ao. The propose of this study was to screen halophilic bacteria from the isolated strains and analyzed these bacteria for their diverse genera and species. There only 41 strains of 113 isolates were halophilic gram positive bacteria. According to Na+ requirement, endospore production, cell morphology, glucose fermentation and colony morphology, 41 strains were divided into 11 groups: the strains of group 1-8 required Na+ for growth, the strains of group 9-11 were not ; and only the strains of group 6 and 9 were facultatively anaerobes which were able to ferment glucose, the strains of other 9 groups were aerobe which were unable to ferment glucose. Group 1 contained two strains, which were the only group producing endospores. The cells of this bacterial group were regular rods and grew into orange circular colonies. Group 2 only included one strain, which could not ferment glucose and was characterized by its orange circular colonies and regular rod cells like group 1. However, the production of endospore was not observed in group 2, a distinction between group 1 and group 2. Group 3 only included one strain, which grew into pink circular colonies and utilized glucose as sole carbone source for growth, a distinction between group 3 and group 1-2. Group 4 contained 4 strains and group 5 contained 15 strains, which could not ferment glucose and the cells of this group were regular rod like group 1-3. However, the production of irregular gray colonies and white circular colonies separatly, a distinction between group 4-5 and group 1-3. Group 6 only included one strain, which were the only group grew into colorless circular colonies, ferment glucose and the cells of this group were irregular rods, a distinction between group 6 and group 1-5. Group 7 contained 2 strains, which the cells of this group were irregular rods, and the strain could not growthed in complex-medium whithout ultra Mg2+, a distinction between group 7 and other 10 groups. Group 8 only included one strain, which the cells of this group were irregular rods, and the only group grew into yellow colonies. Group 9 contained two strains, which the cells of this group were irregular rods, grew into white circular colonies and could not ferment glucose. Group 10 only included one strain, which were the only group grew into red circular colonies, and the cells of this group were regular rods. Group 11 contained 11 strains, which the cells of this group were regular rods,and grew into irregular gray colonies beside I strain grew into white circular colonies. The level of 16S rDNA sequence similarity revealed that the strains C1055 and J169 of Group 1 could be considered as Bacillus hwajinpoensis.The strain CS13 of Group 2 could be considered as Halobacillus trueperi. The strain B8 of Group 3, M336 of Group 7, A53 of Group 9 and A57 of Group 6 could be considered to represent a new species. The strains J149 and BS13 of Group 4, J607 and J717 of Group 5, and A2 of Group 11 Stappia aggregata the closest similary The strains A51 of Group 10 and Ba1a8 of Group 8 was identical to Rhodovulum sulfidophilum and Microbacterium ketoreductum, the closest similary. Based on the phylogenetic tree rebuilt by the 16S rDNA sequence analysis, it was revealed that the 11 groups contained at least 8 species in 8 genera. It is valuable to mention a strain in group 7, M336. Unlike other strains, it could grow neither in complex medium without added Mg2+, nor in 100% marine water or 5% NaCl solution. In addition, it was only 94.6% identical to 16S rDNA sequence of Devosia neptuniae, the closest similary. Therefore, there was no doubt M336 might be a new species or even a new genus. In this study, the selected strain in group 3, 4, 5, 6, 7, 9, 10 and 11 displaying Gram-positive was revealed that their closest species were all Gram-negative via 16S rDNA sequence analysis. A further study should be required to clarify why the outcome contradictory to common observation could occur.
Chen, Tsai-Ling, and 陳柴綾. "Feasibility study on ectoine production of halophilic bacteria using Halomonas salina BCRC17875." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/71494544712471773290.
Повний текст джерела元智大學
生物科技與工程研究所
104
A natural cyclic amino acid derivative, ectoine acts as a protective substrate in many bacterial cells as a compatible solute. Synthesized and accumulated by halophilic and halotolerant microorganisms. Preliminary study of our group as well as those stated in literatures indicated that ectoine is an innovative, multi-functional natural compound not only stabilize proteins and other macromolecules, but also a with an effect in protecting Langerhans cells and UV-induced degradation of DNA. Comparison of ectoine concentration among the three strains obtained from Bioresource Collection and Research Center (BCRC) in Taiwan, the best strain for ectoine synthesis is Halomonas salina BCRC17875. Experimental results indicated that 4.5% appears to be the optimal concentration of NaCl, producing a maximum ectoine production of 2968 mg/L by Halomonas salina BCRC17875. Moreover, ectoine production was optimal in batch cultures when the temperature, pH, agitation rate was controlled at 30 ℃, pH 7.5 and 150 rpm, respectively. While the maximum ectoine concentration was increased by 11-folds when the glucose and glutamate both were 4%, the maximum crude ectoine production reached 2968 mg/L. Furthermore, the chemical structure and molecular masses of the purified ectoine were confirmed by nuclear magnetic resonance (1H-NMR) spectrometer and fast atom bombardment mass (FAB-MS) spectrometer, respectively.
簡俊伊. "Isolation and identification of halophillc bacteria and archaea in Taiwan." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/85949948078726431654.
Повний текст джерелаSoares, Bruno Miguel Ribeiro. "Effect of halophilic bacteria from Aveiro salt pans in the attenuation of saline stress in plants." Master's thesis, 2018. http://hdl.handle.net/10773/25368.
Повний текст джерелаA salinização dos solos é um problema crescente a nível global e têm sido várias as abordagens propostas para atenuar os seus efeitos na produtividade de plantas de interesse económico. O uso de bactérias halófilas ou halotolerantes como promotoras do crescimento de plantas, é uma das estratégias preconizadas para a mitigação do stresse salino. No entanto, são normalmente usadas como inóculo bactérias halotolerantes isoladas da rizosfera de plantas halófitas. O objetivo deste trabalho foi avaliar o potencial de bactérias halófilas, isoladas de uma marinha de sal, na atenuação do stresse salino em Lactuca sativa, usada como modelo de glicófita de interesse agrícola. Uma coleção de estirpes isoladas da marinha de Santiago da Fonte (Aveiro) representando os géneros Bacillus, Halobacillus, Idiomarina e Marinobacter, foi analisada quanto a algumas caraterísticas consideradas como vantajosas na colonização e promoção do crescimento de plantas. Testou-se a produção de enzimas extracelulares em salinidades 0, 20 e 100 de NaCl bem como a capacidade para solubilizar fosfato e produzir ácido 1-aminociclopropano-1-carboxilato desaminase. H. locisalis e I. seosinesis, considerados como mais interessantes face às características promotoras do crescimento, foram testados separadamente e em conjunto, como inóculo em sementes de alface. Foi aplicado um desenho experimental fatorial para testar o efeito da inoculação e da salinidade da água de irrigação sobre a eficiência de germinação das sementes e crescimento das plantas. A eficiência de germinação foi fortemente afetada pela salinidade não tendo sido observados efeitos significativos de nenhum dos inóculos testados. Na condição de salinidade 10, a eficiência de germinação foi mais baixa do que com salinidade 0 e o peso das plantas foi significativamente menor nas plantas inoculadas com o consórcio de isolados do que nas plantas não inoculadas. As plantas inoculadas com o consórcio e cultivadas na salinidade 10 apresentaram menor teor de água. As plantas inoculadas separadamente com H. locisalis ou com I. seosinesis cultivadas em salinidade 0, revelaram um aumento do tamanho das folhas relativamente ao controle não inoculado. Embora não tenham sido encontradas evidências de atenuação do stresse salino, o inóculo H. locisalis apresentou um efeito positivo no crescimento das plantas em condições não-salinas, o que demonstra um potencial como bactéria promotora do crescimento de plantas de interesse agrícola.
Projeto nº 029736 - Programa Operacional Regional do Centro (02/SAICT/2017).
Mestrado em Ecologia Aplicada
Lin, Chung-feng, and 林群峰. "Diversity of halophilic purple non-sulfur bacteria and their capacity to produce renewable resources assessment." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/36686000356405001529.
Повний текст джерела東吳大學
微生物學系
100
Purple non-sulfur bacteria are a group of extraordinary metabolic diverse bacteria, therefore may widely exist in the environment. The study the diversity of freshwater environment of the purple non-sulfur bacteria have been many reported, however, the diversity of the hemophilic purple non-sulfur bacteria are less studied. So this study target separation of pure strains of purple non-sulfur bacteria in different environments of Taiwan western coast, and the use of molecular biological methods for classification, differences strains caused by different environmental , isolated producing hydrogen and Polyhydroxybutyrate (PHB) capacity assessment. Experimental screening out 34 purple non-sulfur bacteria, of which three for halotolerant of purple non-sulfur bacteria, others were all the halophilic of purple non-sulfur bacteria. These bacteria using 16S rDNA sequences sequencing, they were all Rhodovulum sulfidphilum. Strain differences in colony size and bacteria liquid on the color rendering. Use 16S-23S ITS RFLP, 16S rDNA sequence similarity and relationship between phylogenetic tree, 16S-23S ITS (ribosomal intergenic spacer;ITS) sequence similarity and genetic relationship between trees and multi locus sequence typing (the Multiocus sequence type) trying to categorize strains do,but it can do no further grouping. Hydrogen parts, first detected using PCR method screening while nifD and nifH gene of strains, and gas production test, found under the strains of malic acid as carbon sources capable of producing gas. For gas analysis, recognizing the hydrogen gas generated, then selection two strains one was hydrogen yield the highest R. sulfidphilum SSa7, another was gas yield the higher then others R. sulfidphilum SSa3. Maximize hydrogen production efficiency of 6.31ml/h/L and 2.72/ml/h/L, respectively. PHB production parts, strain detected phaC gene using PCR method and then quantitative PHB. Isolated PHB yield were 6~15 (mg/g fresh wet) with a known PHB production ability of Alcaligenes latus 61.60 (mg/g fresh wet) comparative ability is poor. Wastewater section, since waste water usually family livelihood is a fresh water environment. Using halotolerant strains R. sulfidphilum SSa7 to test whether reduce the total organic carbon and to produce hydrogen and producing PHB. It could reduce total organic carbon and produced PHB without hydrogen in wastewater. The future can expect R. Sulfidphilum whole genome sequencing, we are convenient to classification this strain. PHB production and hydrogen-producing part trying to find the most suitable conditions to increase the yield. Strains are using in food or a leather factory, to test if it is capable of producing hydrogen and producing PHB.