Дисертації з теми "GPC1"
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Blankenship, Elise. "Conserved solvent networks in GPCR activation." Case Western Reserve University School of Graduate Studies / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=case1458221506.
Повний текст джерелаPoudel, Sagar. "GPCR-Directed Libraries for High Throughput Screening." Thesis, University of Skövde, School of Humanities and Informatics, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-29.
Повний текст джерелаGuanine nucleotide binding protein (G-protein) coupled receptors (GPCRs), the largest receptor family, is enormously important for the pharmaceutical industry as they are the target of 50-60% of all existing medicines. Discovery of many new GPCR receptors by the “human genome project”, open up new opportunities for developing novel therapeutics. High throughput screening (HTS) of chemical libraries is a well established method for finding new lead compounds in drug discovery. Despite some success this approach has suffered from the near absence of more focused and specific targeted libraries. To improve the hit rates and to maximally exploit the full potential of current corporate screening collections, in this thesis work, identification and analysis of the critical drug-binding positions within the GPCRs were done, based on their overall sequence, their transmembrane regions and their drug binding fingerprints. A proper classification based on drug binding fingerprints on the basis for a successful pharmacophore modelling and virtual screening were done, which facilities in the development of more specific and focused targeted libraries for HTS.
Majin, Wodu. "Mathematical modelling of GPCR-mediated calcium signalling." Thesis, University of Nottingham, 2012. http://eprints.nottingham.ac.uk/12451/.
Повний текст джерелаTang, Lisa Sarah. "GPCR expressions in Saccharomyces cerevisiae : engineering transductions." Thesis, University of Leeds, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.423190.
Повний текст джерелаMishra, Satyakam. "Frequent Subgraph Mining Analysis of GPCR Activation." Case Western Reserve University School of Graduate Studies / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1613575702373053.
Повний текст джерелаSladek, Barbara. "Structural studies of integral membrane GPCR accessory proteins." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:09bf7ada-8e58-49f4-a979-bcd0cec95e8b.
Повний текст джерелаRichardson, Kathryn. "Mechanisms of GPCR signal regulation in fission yeast." Thesis, University of Warwick, 2014. http://wrap.warwick.ac.uk/63554/.
Повний текст джерелаGoddard, Alan David. "Functional analysis of GPCR signalling cascades in Schizosaccharomyces pombe." Thesis, University of Warwick, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.437696.
Повний текст джерелаKoyama, Hiroyuki. "Comprehensive Profiling of GPCR Expression in Ghrelin-producing Cells." Kyoto University, 2016. http://hdl.handle.net/2433/215953.
Повний текст джерелаKiess, Alexandra. "Funktionelle Relevanz intrazellulärer Splicevarianten des Brain-specific Angiogenesis Inhibitor 2 (BAI2)." Doctoral thesis, Universitätsbibliothek Leipzig, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-156171.
Повний текст джерелаGuerrero, Hernández Martina. "Targeting tumor microenvironment crosstalk through GPCR receptors and PI3K pathway." Doctoral thesis, Universitat de Barcelona, 2019. http://hdl.handle.net/10803/667975.
Повний текст джерелаEl estudio del microambiente tumoral está ganando importancia en las últimas décadas debido a su contribución en la formación y desarrollo del cáncer, además de contribuir en la resistencia de las células tumorales a diferentes terapias. Este microambiente interactúa con las células tumorales y activa diferentes vías. El linfoma folicular (FL), es el linfoma no Hodgkin indolente más común y con mayor dependencia del microambiente tumoral, además es considerado incurable. PI3K desempeña un papel importante en la comunicación con el microambiente, y es importante en múltiples funciones celulares, además de contribuir en la angiogénesis, reclutamiento de células inflamatorias y promover el crecimiento tumoral. Idelalisib es un inhibidor de PI3K (específicamente de la isoforma δ), que se aprobó en 2014 por la FDA. Paralelamente la desregulación de BCL2 es primordial en la patogénesis de FL, como consecuencia de la t (14; 18), presente en un 85% de los pacientes, y por lo tanto es un objetivo atractivo para novedosos enfoques terapéuticos. Venetoclax (ABT-199, AbbVie) es un pequeño inhibidor de BCL2, que mostró unos resultados del primer ensayo clínico no satisfactorios (respuesta global del 38%). De este primer estudio concluimos que Idelalisib interfiere en la comunicación de FL y su microambiente inmune, además potencia la actividad de venetoclax atacando a las células tumorales, lo que representa una terapia de combinación prometedora que puede mejorar el resultado del tratamiento de FL.
Hollier, Mark John. "Properties of the C-terminal tail of HIV-1 gp41." Thesis, University of Warwick, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.408463.
Повний текст джерелаGardner, Jacob Andrew. "GPCR Signaling in the Genesis and Progression of Pancreatic Cancer." Diss., Temple University Libraries, 2009. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/35453.
Повний текст джерелаPh.D.
Ductal adenocarcinomas of the pancreas are the 4th most common cause of cancer death. The 1 and 5 year survival rates for all stages combined are currently 26% and 5% respectively. Median survival is less than 6 months. Despite remarkable progress in the fields of genetics, cancer biology, and advances in surgical techniques as well as chemotherapeutics, our ability to recognize and treat patients with pancreatic cancer remains poor. GPCR signaling modules have been increasingly implicated in the genesis and progression of pancreatic cancers. Aberrant agonist production, receptor expression and dysfunctional signaling resulting from genomic instability in a background of a heterotopic tumor-stromal microenvironment, contribute to the initiation, progression, and eventual metastasis of the disease. Numerous GPCR agonists, including lysophosphatidic acid (LPA), along with their cognate receptors have been implicated in this oncogenic process. LPA, one of the simplest bioactive lipids, has been shown to be a potent stimulant of metastatic behavior in in vitro models. It also acts as a mitogen by inducing proliferation and cell survival pathways in various normal and transformed cell lines. In patients with pancreatic cancer both the receptors and ligand have been found to be overexpressed. It has been noted that pancreatic cancer cell lines expressing higher levels of the LPA receptors present with greater motility. This has led to the hypothesis that LPA contributes to the progression of pancreatic cancer through the promotion of a metastatic phenotype. However, the underlying mechanisms have not been well described. LPA receptors have been shown to couple to the Gi, Gq, or G12 family of heterotrimeric G proteins. Consequently, signals transduced through these receptors have been shown to stimulate Gαi, Gαq, and Gα12/13 dependent pathways. While earlier studies have linked Gαi to LPA induced migration, there is recent evidence to suggest that Gα13 may provide a major signaling mechanism for LPA receptors stimulating migration in diverse cell types including cancer cell lines. Given the ominous nature of pancreatic cancers it is of critical importance to understand the mechanisms that promote more malignant phenotypes and to assess the role of Gα13 in this process. The goal of this thesis therefore is to define the role of Gα13 in LPA-mediated migration of pancreatic cancer cells. To assess the oncogenic potential of LPA and the role of Gα13 in stimulating the migration of pancreatic cancer cells, a panel of pancreatic cancer cell lines was assembled and characterized with regard to their expression of the LPA receptors as well as the Gα subunits of the heterotrimeric G proteins. These cell lines were further studied through a series of proliferation, wound healing, and transwell migration assays to assess the role of LPA in the induction of proliferation and migration in pancreatic cancer cells. The results demonstrated that LPA functions as a mitogen in certain pancreatic cancer cell lines, but is a potent stimulant of cell motility and invasive migration. Interestingly, these studies indicated that this response proceeds through routes that may not involve Gαi, as a potent migratory response was observed in MDAPanc28 cells which lack expression of the Gαi subunit. This was verified through the transwell assays conducted in the presence of PTX demonstrating that migration occurs independently of PTX sensitive mechanism and thus independently of Gαi.. Using a dominant negative mutant strategy, the studies presented in this thesis establishes the role of Gα13 in mediating LPA-LPAR stimulated migration of pancreatic cancer cells. Using pancreatic cancer cell lines that stably express the competitively inhibitory dominant negative mutant of Gα13, the ability of these mutants to inhibit a LPA mediated migratory response was monitored by wound-healing as well as transwell migration assays The results of these studies indicated a substantial attenuation of the migratory response and demonstrated for the first time the critical role of Gα13in LPA induced migration in a pancreatic cancer cell line.
Temple University--Theses
Senarath, Kanishka D. "Interrogation of GPCR-G Protein Signaling using Novel Optogenetic Tools." University of Toledo / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=toledo155681039815937.
Повний текст джерелаBehrendt, Rayk. "Immunogene und immunsuppressive Eigenschaften des transmembranen Hüllproteins gp41 von HIV." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2009. http://dx.doi.org/10.18452/15993.
Повний текст джерелаThe development of an effective HIV vaccine is considered the to play a key role in controlling the HIV pandemic. Conventional immunisation strategies using recombinant envelope proteins of the virus did not lead to the induction of a broad range protective immunity. A new target sequence for the induction of a broadly neutralising humoral immune response has been discovered through the characterization of the gp41 specific broadly neutralising monoclonal antibodies 2F5 and 4E10. Until now all attempts to induce 2F5/4E10 like neutralising antibodies failed. So far only the linear main epitope (E2) of 2F5 and 4E10, located in the C-terminal part of the gp41 ectodomain was used as the target sequence. However, it was recently shown that an N-terminal domain (E1) of gp41 increases the avidity of 2F5 to its epitope. The E1 domain may therefore be involved in the mediation of a neutralisation active binding. For the first time immunisation strategies have been developed that target both previously identified domains (E1 and E2) of gp41. The sequences corresponding to E1 and E2 have been introduced at homologous positions in the structurally related transmembrane envelope protein p15E of the Koala Retrovirus (KoRV). These generated hybrid antigens have been used for immunisation of wistar rats. They were applied as recombinant proteins expressed in E.coli and as DNA using a ballistic immunisation (GeneGun®) approach. Although in first trials neutralising antibodies specific for gp41 of HIV-1 were induced, these results could not be reproduced. Analysis of the induced antibodies showed a shift of their binding specifity. A bacterial infection of the used animals was identified as the cause of the unexpected shift in the antigen specific humoral immune response. For evaluation of the immunisation studies a new neutralisation assay based on the measurement of provirus integration by duplex real time PCR has been extended for controls of virus specifity and cytotoxicity.
BAUDET, SYLVIE. "Etude cristallographique du complexe barnase-d(gpc)." Paris 11, 1990. http://www.theses.fr/1990PA112016.
Повний текст джерелаAdamson, Roslin Jane. "Probing GPCR-Gα interactions : a functional study by EM and SPR". Thesis, University of Oxford, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.669745.
Повний текст джерелаMason, Vicki Louise. "Effects of different assay configurations on pharmacological profiling of GPCR targets." Thesis, University of Reading, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.494820.
Повний текст джерелаValsechi, Marina Curado [UNESP]. "Análise funcional do gene GPC3 em carcinoma renal de células claras." Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/127546.
Повний текст джерелаFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
GPC3 (Glipican-3) é membro de uma família de proteoglicano de heparina sulfatada (HSPG). O GPC3 pode atuar controlando a migração celular, regulação negativa do crescimento celular e indução de apoptose. Esse gene é relatado por estar hipoexpresso em vários tipos de cânceres, o que pode resultar no crescimento celular descontrolado e contribuir para o fenótipo maligno de alguns tumores. O objetivo desse estudo foi analisar o mecanismo de ação do gene GPC3 em carcinoma renal de células claras (CCRCC). Primeiramente, foi construído o vetor de expressão e transfectado nas linhagens celulares de carcinoma renal ACHN e 786-O. A expressão de GPC3 foi analisada usando qRT-PCR e imunohistoquímica. A proliferação celular foi avaliada usando o MTT e o ensaio de formação de colônia. Análises de apoptose e ciclo celular foram avaliadas por citometria de fluxo. Foi observado que o gene GPC3 estava com baixa expressão em amostras de carcinoma renal de células claras e nas linhagens celulares quando comparado com amostras renais normais. Foi observado que a expressão de RNAm e os níveis de proteína GPC3 aumentaram após a transfecção com o vetor de expressão contendo a ORF de GPC3 nas linhagens celulares. A taxa de proliferação celular diminuiu nas células superexpressando GPC3 em ambas as linhagens, ACHN e 786-O (p<0,01). A apoptose não foi observada nas linhagens celulares de carcinoma renal superexpressando GPC3 (p > 0,05); entretanto, ocorreu um aumento na população de células na fase G1 do ciclo celular (p< 0,05). Esses resultados sugerem que o gene GPC3 reduz a taxa de proliferação celular por meio da parada do ciclo celular na fase G1 em carcinoma renal
Background: GPC3 (Glypican 3) is a member of the family of glypican heparan sulfate proteoglycans (HSPG). The GPC3 gene may play a role in controlling cell migration, negative regulation of cell growth and induction of apoptosis. This gene is reported to be downregulated in several cancers, which can result in uncontrolled cell growth and which can also contribute to the malignant phenotype of some tumors. The purpose of this study was to analyze the mechanism of action of the GPC3 gene in clear cell renal cell carcinoma (CCRCC). Methods: First, we constructed the expression vector and transfected renal carcinoma cell lines. GPC3 expression was analyzed using qRT-PCR and immunohistochemistry. We evaluated cell proliferation using MTT and colony formation assays. Apoptosis and cell cycle analyses were evaluated using flow cytometry. Results: We observed that the GPC3 gene was downexpressed in the clear cell renal cell carcinoma samples and in cell lines, which were both compared to normal renal samples. We observed that GPC3 mRNA expression and protein levels increased after the transfection into ACHN and 786-O cell lines. We found that the cell proliferation rate decreased in cells overexpressing GPC3 in both cell lines, ACHN and 786-O (p < 0.01). Also, apoptosis in the renal cell carcinoma cell line was not observed in cells overexpressing GPC3 (p > 0.05), but there was an increase in the cell population during the G1 phase in the cell cycle (p< 0.05). Conclusion: We suggest that the GPC3 gene reduced the cell proliferation rate through cell cycle arrest during the G1 phase in renal cell carcinoma
Valsechi, Marina Curado. "Análise funcional do gene GPC3 em carcinoma renal de células claras /." São José do Rio Preto, 2013. http://hdl.handle.net/11449/127546.
Повний текст джерелаBanca: Ana Carolina Gomes Jardim
Banca: Wilson Araújo da Silva Júnior
Banca: Ana Elizabete Silva
Banca: Sônia Maria Oliani
Resumo: GPC3 (Glipican-3) é membro de uma família de proteoglicano de heparina sulfatada (HSPG). O GPC3 pode atuar controlando a migração celular, regulação negativa do crescimento celular e indução de apoptose. Esse gene é relatado por estar hipoexpresso em vários tipos de cânceres, o que pode resultar no crescimento celular descontrolado e contribuir para o fenótipo maligno de alguns tumores. O objetivo desse estudo foi analisar o mecanismo de ação do gene GPC3 em carcinoma renal de células claras (CCRCC). Primeiramente, foi construído o vetor de expressão e transfectado nas linhagens celulares de carcinoma renal ACHN e 786-O. A expressão de GPC3 foi analisada usando qRT-PCR e imunohistoquímica. A proliferação celular foi avaliada usando o MTT e o ensaio de formação de colônia. Análises de apoptose e ciclo celular foram avaliadas por citometria de fluxo. Foi observado que o gene GPC3 estava com baixa expressão em amostras de carcinoma renal de células claras e nas linhagens celulares quando comparado com amostras renais normais. Foi observado que a expressão de RNAm e os níveis de proteína GPC3 aumentaram após a transfecção com o vetor de expressão contendo a ORF de GPC3 nas linhagens celulares. A taxa de proliferação celular diminuiu nas células superexpressando GPC3 em ambas as linhagens, ACHN e 786-O (p<0,01). A apoptose não foi observada nas linhagens celulares de carcinoma renal superexpressando GPC3 (p > 0,05); entretanto, ocorreu um aumento na população de células na fase G1 do ciclo celular (p< 0,05). Esses resultados sugerem que o gene GPC3 reduz a taxa de proliferação celular por meio da parada do ciclo celular na fase G1 em carcinoma renal
Abstract: Background: GPC3 (Glypican 3) is a member of the family of glypican heparan sulfate proteoglycans (HSPG). The GPC3 gene may play a role in controlling cell migration, negative regulation of cell growth and induction of apoptosis. This gene is reported to be downregulated in several cancers, which can result in uncontrolled cell growth and which can also contribute to the malignant phenotype of some tumors. The purpose of this study was to analyze the mechanism of action of the GPC3 gene in clear cell renal cell carcinoma (CCRCC). Methods: First, we constructed the expression vector and transfected renal carcinoma cell lines. GPC3 expression was analyzed using qRT-PCR and immunohistochemistry. We evaluated cell proliferation using MTT and colony formation assays. Apoptosis and cell cycle analyses were evaluated using flow cytometry. Results: We observed that the GPC3 gene was downexpressed in the clear cell renal cell carcinoma samples and in cell lines, which were both compared to normal renal samples. We observed that GPC3 mRNA expression and protein levels increased after the transfection into ACHN and 786-O cell lines. We found that the cell proliferation rate decreased in cells overexpressing GPC3 in both cell lines, ACHN and 786-O (p < 0.01). Also, apoptosis in the renal cell carcinoma cell line was not observed in cells overexpressing GPC3 (p > 0.05), but there was an increase in the cell population during the G1 phase in the cell cycle (p< 0.05). Conclusion: We suggest that the GPC3 gene reduced the cell proliferation rate through cell cycle arrest during the G1 phase in renal cell carcinoma
Doutor
Watson, Douglas Stuart. "Lipopeptide immunogens targeting the membrane proximal region of HIV-1 gp41." Diss., Search in ProQuest Dissertations & Theses. UC Only, 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3359565.
Повний текст джерелаSource: Dissertation Abstracts International, Volume: 70-06, Section: B, page: 3649. Adviser: Francis C. Szoka.
Schafranski, Luiz Erley. "O protótipo GPCP-1 :: jogo do planejamento e controle da produção /." Florianópolis, SC, 1998. http://repositorio.ufsc.br/xmlui/handle/123456789/77907.
Повний текст джерелаMade available in DSpace on 2012-10-17T08:35:11Z (GMT). No. of bitstreams: 0Bitstream added on 2016-01-08T23:14:42Z : No. of bitstreams: 1 139965.pdf: 20661614 bytes, checksum: 854c64c7aba1de2e3c550cc4b5bf3cb2 (MD5)
Saunders, Charles William. "Characterization of cellulose esters via GPC/FT-IR." Diss., This resource online, 1990. http://scholar.lib.vt.edu/theses/available/etd-07282008-135448/.
Повний текст джерелаPIONTKUSKY, FILHO A. J. "Proposta de GPC Auto-Tunning Usando Algoritmo Genético." Universidade Federal do Espírito Santo, 2018. http://repositorio.ufes.br/handle/10/9564.
Повний текст джерелаNão disponível.
Engemaier, Eva. "Die strukturelle und funktionelle Evolution des G-Protein-gekoppelten Rezeptors GPR34." Doctoral thesis, Universitätsbibliothek Leipzig, 2012. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-86270.
Повний текст джерелаThamm, Markus. "Charakterisierung der Serotonin-Rezeptoren der Honigbiene Apis mellifera : von den Genen zum Verhalten." Phd thesis, Universität Potsdam, 2009. http://opus.kobv.de/ubp/volltexte/2010/4073/.
Повний текст джерелаThe serotonergic system plays an important role in the control and modulation of many physiological and behavioral processes in both vertebrates and invertebrates. In the honeybee Apis mellifera, serotonin (5-hydroxytryptamine, 5-HT) has been implicated in the control and regulation of division of labor as well as learning and memory. A key role in understanding the serotonergic system plays the molecular and functional characterization of 5-HT receptor subtypes. In most cases, serotonin receptors represent G protein-coupled receptors (GPCRs). This work describes the characterization of honeybee serotonin receptors. This comprises the identification of their molecular structure, intracellular second messenger pathways, pharmacological properties, expression profiles and functions. By screening the honeybee genome, we found three candidate genes encoding for putative serotonin receptors. The cDNAs of these genes were cloned and the deduced amino acid sequences were analysed. The sequence information was used to isolate the cDNAs encoding for these three receptors. Comparison of the deduced amino acid sequences with sequences of other known receptors suggests that one receptor belongs to the 5-HT1 (Am5-HT1) and the other two receptors to the 5-HT2 receptor class (Am5-HT2α and Am5-HT2β). Major characteristics common to all GPCRs (e.g. the heptahelical architecture) were confirmed by structural analyses of the deduced amino acid sequences. Furthermore, truncated receptor transcripts representing alternative splice variants of both 5-HT2 receptors could be detected. HEK293 cells were stably transfected with the cDNAs of Am5-HT1 or Am5-HT2_ and functionally and pharmacologically analysed. The activation of Am5-HT1 by 5-HT results in the dose dependent attenuation of adenylyl cyclase activity. 5-methoxytryptamine (5-MT) and 5-carboxamidotryptamine are able to imitate the 5-HT effect. In contrast, methiothepin is able to block the entire 5-HT effect, whereas prazosine and WAY100635 block the 5-HT effect only partially. The Am5-HT2α receptor stimulates the synthesis of the second messenger inositol trisphosphate which in turn mediates an increase in the intracellular Ca2+. The substances 5-MT and 8-OH-DPAT were identified as agonists of the Am5-HT2α receptor. In contrast, clozapine, methiothepine, mianserine, and cyproheptadine show strong antagonistic actions. A truncated alternative splice variant of the Am5-HT2α-receptor was also analysed but didn’t show any functional coupling by itself. An antiserum was raised against the third cytoplasmic loop (CPL3) of the Am5-HT1 receptor. This antiserum detects a protein with a molecular mass of 50 kDa in western blot analyses. The expression of the Am5-HT1 receptor was studied in detail using immunohistochemistry. Strong Am5-HT1-like immunofluorescence was observed in the ocellar nerve, in the three optic ganglia and in the α- and β-lobes, the pedunculi, the lip and the basal ring of the mushroom bodies. Furthermore, co-labeling with an antibody against 5-HT showed that this receptor is expressed in close vicinity to serotonergic neurons. Finally, behavioral experiments suggest a possible role of the Am5-HT1 receptor in phototactic behavior. Feeding of 5-HT to worker honeybees results in a decrease of phototactic behavior. This 5-HT action could be mimiced by feeding of the Am5-HT1 agonist 5-CT. In contrast, the Am5-HT1 antagonist prazosine prevents the 5-HT-induced decrease in phototaxis.
Zhang, Boyang. "Functional and Structural Insights into the First and Second Intracellular Domains for D1-Class Dopaminergic Receptors." Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/35932.
Повний текст джерелаGentsch, Marcus. "Funktionelle Analyse und Charakterisierung des Gpr1-Proteins in der Hefe Yarrowia lipolytica." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2003. http://nbn-resolving.de/urn:nbn:de:swb:14-1071153552203-80428.
Повний текст джерелаJaén, Cristina. "Differential coupling of RGS3s and RGS4 to GPCR-GIRK channel signaling complexes." [Tampa, Fla] : University of South Florida, 2006. http://purl.fcla.edu/usf/dc/et/SFE0001533.
Повний текст джерелаAltosaar, Katrin. "Dimer-dependent allosteric modulation within GPCR signalling complexes can influence signalling diversity." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=114353.
Повний текст джерелаLes récepteurs couplés aux protéines G (RCPG) constituent le plus grand groupe de récepteurs de la surface cellulaire, qui traduisent les signaux environnementaux en réponses cellulaires via leurs protéines G associées. Contrairement à notre compréhension initiale, la majorité des RCPG ne fonctionnent pas en tant que monomères, mais possiblement en tant que dimères ou même oligomères. Les approches actuelles de conception de médicament estiment que lors de la liaison d'un médicament aux deux récepteurs d'un dimère quelconque, ces derniers fonctionnent potentiellement indépendamment l'un de l'autre. Cependant, cette notion a été reconsidérée par une étude récente montrant que la liaison d'un ligand aux deux récepteurs peut les altérer par voie de communication allostérique. Alors qu'un premier récepteur peut être requis pour initialiser la signalisation, un second peut contrôler ou modifier ces signaux, n'ayant pas nécessairement une signalisation directe comme résultante. Dans l'étude suivante, basée sur la notion de modulation allostérique au sein d'homodimère et d'hétérodimère, les changements de signalisation en aval ainsi qu'au niveau du complexe récepteur/protéine G/effecteur (RGE) ont été étudiés et comparés en réponse à différentes combinaisons de ligands pour chaque protomère. En utilisant une combinaison d'essais de signalisation de calcium, d'adénosine monophosphate cyclique (cAMP) et de protéine kinase activée par des agents mitogènes (MAPK), une interaction fonctionnelle entre le récepteur dopaminergique D2 et le récepteur de l'ocytocine (D2R/OTR) a été démontrée dans les cellules HEK 293. Des expériences d'immunoprécipitation, de transfert d'énergie de résonance par bioluminescence (BRET) et de microscopie confocale ont révélé la présence d'hétérodimère entre le D2R et l'OTR in vitro, ce qui pourrait expliquer la nature des interactions fonctionnelles allostériques. En utilisant la technique de BRET, la dynamique fonctionnelle du complexe RGE dans les cellules HEK 293 a été examinée chez deux autres hétérodimères, soit celui composé du récepteur adrénergique β2 et du récepteur cannabinoïde CB1 (β2AR/CB1R) et l'hétérodimère β2AR/OTR, afin de déterminer comment ils traduisent les évènements de signalisation. Ces études démontrent donc qu'une interaction fonctionnelle peut survenir sur le plan de la conformation du complexe de signalisation. Par conséquent, la signalisation d'un RCPG peut être modulée par son récepteur partenaire au niveau des effecteurs ou au niveau du complexe de signalisation lui-même. Pour cette raison, il serait impératif de réanalyser in vivo les propriétés allostériques d'hétérodimères putatifs, ce qui pourrait expliquer certains effets secondaires d'une multitude de médicaments et ce qui pourrait impliquer des changements majeurs dans la façon de concevoir de nouveaux médicaments.
Hall, Caroline Jane. "The effect of GPCR crosstalk on intracellular Ca2+ responses and downstream signalling." Thesis, University of Leicester, 2011. http://hdl.handle.net/2381/9528.
Повний текст джерелаJaén, Cristina. "Differential coupling of RGS3s and RGS4 to GPCR-GIRK channel signaling complexes." Scholar Commons, 2006. http://scholarcommons.usf.edu/etd/2571.
Повний текст джерелаRanganathan, Anirudh. "The impact of GPCR structures on understanding receptor function and ligand binding." Doctoral thesis, Stockholms universitet, Institutionen för biokemi och biofysik, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-129879.
Повний текст джерелаAt the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 3: Manuscript.
Ebersold, Anne. "Production d'anticorps monoclonaux humains dirigés contre la gp41 du virus HIV-1." Lyon 1, 1992. http://www.theses.fr/1992LYO1T268.
Повний текст джерелаBahena, Silvia. "Computational Methods for the structural and dynamical understanding of GPCR-RAMP interactions." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-416790.
Повний текст джерелаSalcedo, Romero de Ávila José Vicente. "GPCs en espacio de estados para el control de sistemas no lineales." Doctoral thesis, Universitat Politècnica de València, 2008. http://hdl.handle.net/10251/1882.
Повний текст джерелаSalcedo Romero De Ávila, JV. (2005). GPCs en espacio de estados para el control de sistemas no lineales [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/1882
Palancia
Scarlin, Hugh. "Studies on the synthesis of novel PP2A inhibitors and a GPCR detergent." Thesis, University of Glasgow, 2016. http://theses.gla.ac.uk/7370/.
Повний текст джерелаElenko, Eric. "Localization and fate of GAIP following G[alpha]i̳ linked GPCR stimulation /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2001. http://wwwlib.umi.com/cr/ucsd/fullcit?p3026385.
Повний текст джерелаCornélio, Filho Plínio. "O modelo de simulação do GPCP-1 : planejamento e controle da produção." reponame:Repositório Institucional da UFSC, 1998. https://repositorio.ufsc.br/handle/123456789/111375.
Повний текст джерелаJha, Ankita. "Quantitative control of GPCR organization and signaling by endocytosis in epithelial morphogenesis." Thesis, Aix-Marseille, 2017. http://www.theses.fr/2017AIXM0393/document.
Повний текст джерелаDuring Drosophila gastrulation, apical activation of the actomyosin networks drives apical constriction in the invaginating mesoderm and cell-cell intercalation in the extending ectoderm. Here, we show that cell-surface G-protein coupled receptor, Smog activates G-proteins, Rho1 and Rho-kinase that is required for apical constriction and cell-cell intercalation. Quantitative control over GPCR activity and thereby Rho1 activation underlies differences in deformation of the mesoderm and ectoderm cells but the mechanisms remain elusive. We show that GPCR-Smog activity is concentrated on two different apical plasma membrane compartments i.e. the surface and the plasma membrane invaginations. Using FCS, we probe the surface of the plasma membrane (PM) and show that Smog homo-clusters in response to its activating ligand Fog. Endocytosis of Smog is facilitated by the kinase Gprk2 and the adaptor protein β-Arrestin-2 that clears active Smog from the surface of PM. When Fog concentration is high or endocytosis is low, Smog arranges in homo-clusters and accumulates in plasma membrane invaginations (PMI), that are hubs for Rho1 activation. Lastly, we find high Smog homo-cluster concentrations and numerous apical PMIs in the mesoderm compared to the ectoderm. We identify that dynamic partitioning of active Smog on the surface of the PM or PMI directly impact on Rho1 signaling. PMIs accumulate high Rho1-GTP suggesting they form signaling centers. Fog concentration and Smog endocytosis form coupled regulatory processes that regulate quantitative differential Rho1/MyoII activation in the Drosophila mesoderm and ectoderm
Zanella, Júnior Aldo. "Experimentações práticas e simuladas de controle preditivo generalizado - GPC." Universidade do Estado de Santa Catarina, 2015. http://tede.udesc.br/handle/handle/2084.
Повний текст джерелаCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
This work introduces the report of performed studies in order to evaluate the applicability of generalized predictive control (GPC) to several plants. The main goal is to analyze the GPC performance in processes with different features, analyzing the influence of its tuning parameters. The study is justified by the fact that GPC presents itself as a generalized solution for several classes of processes, which are becoming increasingly complex and demanding for traditional controllers to handle. For the purpose to prove this proposal of GPC, it was performed several tests with plants of different orders and response characteristics, real and simulated, varying controller tuning parameters and measuring some quality indices. It was evaluated the influence of tuning parameters and it was made a report of conclusions that was reached. Through obtained results, it is shown that GPC satisfies the proposal and presents favorable results.
Esta dissertação traz o relato do estudo realizado a fim de avaliar a aplicabilidade do controlador preditivo generalizado (GPC) em plantas diversas. O objetivo principal é analisar o desempenho do GPC em processos com diferentes características, analisando a influência dos seus parâmetros de sintonia. O estudo se justifica pelo fato de que o GPC apresenta-se como uma solução generalizada para diversos tipos de processos, os quais estão se tornando cada vez mais complexos e com maiores exigências para o controlador. A fim de comprovar essa proposta do GPC, realizou-se inúmeros ensaios com plantas com respostas e ordem diferentes, reais e simuladas, variando-se os parâmetros de sintonia do controlador e medindo-se alguns parâmetros de qualidade. Avaliou-se a influência dos parâmetros de sintonia e fez-se um relato das conclusões a que se chegou. Através dos resultados obtidos, mostra-se que o GPC corresponde ao que se propõe para as plantas testadas e apresenta resultados favoráveis.
Cruz, Daniel Miranda. "Estruturas de controle preditivo repetitivo baseadas na formulação GPC." reponame:Repositório Institucional da UFSC, 2015. https://repositorio.ufsc.br/xmlui/handle/123456789/136484.
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Este trabalho apresenta um estudo de diversas estruturas de controle baseadas no algoritmo de controle preditivo generalizado, Generalized Predictive Control (GPC), e no controle repetitivo, Repetitive Control (RC). Os controladores analisados possuem as vantagens do controle preditivo no que diz respeito à otimização e ao tratamento de restrições e as do controle repetitivo no tratamento de perturbações periódicas, caracterizando a estrutura de controle preditivo generalizado repetitivo, Repetitive Generalized Predictive Control (RGPC). A estrutura proposta usa ação de controle repetitiva, baseada em um modelo interno com filtro de robustez e faz o tratamento das restrições considerando a repetitividade da ação de controle. O estudo compara diversas possibilidades de implementação, uma clássica e outra com o projeto separado, apontando vantagens e desvantagens de cada uma, assim como a aplicação em um estudo de caso.
Abstract : This work presents a study of different control schemes based on the GPC and RC. These controllers have the advantages of online optimization and contraint hadling of the predictive control and periodic signals treatment of the repetitive control, defining the RGPC. The proposed structure uses a repetitive control action based on an internal model with robustness filter and deals with constraints considering the control action repetitiveness. The study compares various implementation possibilities, a classic and a separated design, pointing advantages and disadvantages of each one as well as their implementation.
Augstein, Antje. "Molekularbiologische Charakterisierung und funktionelle Analyse des GPR1-Genproduktes in der Hefe Yarrowia lipolytica." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2001. http://nbn-resolving.de/urn:nbn:de:swb:14-997694149203-01172.
Повний текст джерелаBrewer, Cynthia. "Use of PC12 cells to characterize PAFR-GPCR-mediated activity in neural precursors." Thesis, University of Ottawa (Canada), 2001. http://hdl.handle.net/10393/9170.
Повний текст джерелаHeidari, Yasin. "Identification and characterisation of a novel isoform of gp91 subunit of NADPH oxidase." Thesis, King's College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.407934.
Повний текст джерелаJackson, Verity. "Mechanistic studies of the adhesion-GPCR latrophilin and its interactions in neural guidance." Thesis, University of Oxford, 2017. http://ora.ox.ac.uk/objects/uuid:ecc6187a-3e20-4753-ada6-dc009ad7f6e2.
Повний текст джерелаCutolo, Pasquale. "Etude de l'interaction structurelle et fonctionnelle entre la chimiokine CXCL12 et ses récepteurs : CXCR4 et ACKR3/CXCR7." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS550/document.
Повний текст джерелаThe axis formed by the chemokine CXCL12 and its receptor CXCR4 is conserved in vertebrates where it plays an important role in embryogenesis and adult life, regulates many processes of immune responses through its functions in cell migration, survival and proliferation.In addition, this axis is involved in pathological processes such as cancers (growth and metastasis) and immune deficiencies and malfunctions (eg deregulated expression, mutations or polymorphisms) and is also hijacked by certain pathogens (eg HIV, human papilloma virus).A large working group is dedicated to this pair as a therapeutic target, but only a compound (ie Plerixafor) achieved approval for clinical use by the potential of this area as a drug target unexplored.Although this axis is the subject of great interest, questions remain about the structural determinants involved in CXCL12 / CXCR4 interaction.However, the recently resolved diffraction structure of CXCR4 gave some clue about these questions, and beyond possible stoichiometry between CXCL12 and CXCR4.Several lines of evidence support the concept that forms CXCR4 homo- and hetero-oligomers (which can contribute to the diversity of the receptor functions), as shown in the diffraction structure, the gain function of a mutant CXCR4 receptor responsible for the syndrome WHIM and allosteric modulation of CXCR4 functions by CXCR7 (ACKR3), the second receptor of the chemokine CXCL12. The ability to form oligomers opens many issues of CXCL12 and its interaction with CXCR4 and CXCR7 / ACKR3.The stoichiometry of this interaction still remains an open question, as the receptor is capable to form oligomers with the same receptor or other receptors, particularly CXCR7 / ACKR3. This receptor, known as scavenger, has not solved structure and the mechanism of interaction with CXCL12 is unknown.To study the interactions CXCL12 / CXCR4 / CXCR7, we applied several molecular modeling techniques such as peptide-peptide docking and molecular dynamics simulations.Objectives of this project were: the resolution of the different stoichiometric forms for the interaction of CXCR4 and CXCL12 (molecular modeling, docking and dynamic); modeling the CXCR7 / ACKR3 receptor structure and its interaction with CXCL12 (homology modeling), with the characterization of domains and residues key in the activation of downstream signaling pathways of the receptor (CXCR7 / ACKR3 mutants); the study and characterization of new innovative tools for the detection of oligomerization of these receptors in endogenous conditions. (Nanobodies, HTRF)The results of the first objective were published in January 2016: PMID 26813575.Modeling of CXCR7 / ACKR3 allowed us to generate several mutants of the receptor to test our hypothesis about the activation pathways.Nanobodies were fully characterized for CXCR4 to be used in a second study to identify oligomeric forms of the receptor in tissues and cells
Sintes-Zydowicz, Nathalie. "Polyimides - amides, microstructure : mécanismes réactionnels : étude par RMN et GPC." Lyon 1, 1991. http://www.theses.fr/1991LYO10165.
Повний текст джерелаMenezes, Marcos André Silveira. ""Implementação de um controlador GPC adaptativo aplicado a processos industriais"." reponame:Repositório Institucional da UFSC, 1993. https://repositorio.ufsc.br/handle/123456789/111637.
Повний текст джерелаMazoco, Bruna Marques. "Proposta de um algoritmo GPC adaptativo com baixo custo computacional." reponame:Repositório Institucional da UFES, 2015. http://repositorio.ufes.br/handle/10/1423.
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Esta dissertação propõe um algoritmo do Controlador Preditivo Generalizado (GPC) com horizonte de controle igual a um para ser aplicado em plantas industriais com modelos variantes no tempo, simples o su ficiente para ser implementado em Controlador Lógico Programável (PLC). A solução explícita do controlador é obtida em função dos parâmetros do modelo e dos parâmetros de sintonia do GPC (horizonte nal de predição hp e o fator de supressão do sinal de controle ), além das entradas e saídas presentes e passadas. A sintonia do fator de supressão e do horizonte de previsão GPC é feita através do lugar das raízes da equação característica do sistema em malha fechada, sempre que os parâmetros do modelo da planta industrial (estável ou instável em malha aberta) forem modificados.
This dissertation proposes a new formulation of the Generalized Predictive Control (GPC) algorithm to be applied in industrial plants with time-varying models. The unitary control horizon premisse alows it to be simple enough to be implemented in a Programmable Logic Controller (PLC). The explicit solution of the control increment is obtained from the parameters of the model and the GPC tuning parameters (prediction horizon hp and supression weight ), in addition to past and present inputs and outputs. Supression weight tuning is done by Root Locus technique, constructed from the system closed loop characteristic polynomium, everytime the model parameters of the industrial plant (stable or not in open loop) su er modi fication.