Дисертації з теми "Glycan code"
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Lopes, Valéria Stefania. "Caracterização da família de genes HSP20 em Glycine max." Universidade Estadual de Londrina, EMBRAPA. Centro de Ciências Exatas. Programa de Pós-Graduação em Biotecnologia, 2012. http://www.bibliotecadigital.uel.br/document/?code=vtls000175506.
Повний текст джерелаThe small heat shock proteins (HSP20) are often associated in plant stress response caused by abiotic factors and, more recently, have also been associated with response to biotic stresses. The Hsp20 genes represent, in plants, the most abundant class among the heat shock proteins, but little is known about this gene family in soybean. Due their apparent multifunctionality, these proteins are promising targets to the crop varieties development for better conditions adapted to biotic and abiotic stresses, even when they are combined. Thus, the present study conducted an in silico molecular characterization of regulatory and coding regions of HSP20 genes from soybean, focus in its genome distribution, subcellular localization, division into subfamilies, secondary structure and regulation under biotic and abiotic stresses, besides the identification patterns to cis elements potentially involved in the response to nematodes. After the exploration of Hsp20 genes annotation in soybean genome databases, 76 gene models were obtained. After in silico analysis, just 52 gene models were part of the GmHsp20 potencial candidates due to their structural characteristics of cis elements and expression profile. In addition, based on in vivo analysis, 45 soybean Hsp20 genes were identified, distributed in 11 subfamilies, for which is possible to observe a specific secondary structure for each one. Among the 45 GmHsp20 genes heat stress responsives, 5 genes were cold stress responsive and other five were nematode infection by M. javanica responsive. Moreover, two genes were observed being responsive to biotic stress, but they weren't responsive to thermal shock. Operational Models of Hsp20 promoters were obtained to responsive genes to each stress condition examined in this study. Among the identified cis elements in Hsp20 soybean genes that were responsive to M. javanica infection were W box, CAAT box, ABRE and MYB, besides the HSE / Heat element. Promoters responsive to biotic stress in soybean follows composition and distribution standards of cis elements, as described in the literature to be related to this type of stress. These results, such as responsive genes and promoters to many different stresses, can assist in generation of expression directed technologies even more advanced and new soybean genotypes more adapted to under combined stress conditions.
Rachid, Breno Francovig. "Identificação de novos locos de resistência à ferrugem asiática (Phakopsora pachyrhizi) em soja (Glycine max)." Universidade Estadual de Londrina. IAPAR. EMBRAPA. Centro de Ciências Biológicas. Programa de Pós-Graduação em Genética e Biologia Molecular, 2008. http://www.bibliotecadigital.uel.br/document/?code=vtls000154641.
Повний текст джерелаIn Brazil, the soybean rust, caused by Phakopsora pachyrhizi, has caused yield losses and increased the cost of production by the intensive use of fungicides in soybean fields. The use of resistant varieties is an important tool to control the disease. Currently five different loci have been reported containing genes for resistance to disease, called rpp1 to rpp5. A new race of the fungus broke the resistance conferred by some genes present in the loci rpp1 and rpp3 in Brazil. This study aimed to perform allelism tests between sources of resistance identified in the germplasm bank of Embrapa Soybean, whose genes do not belong to rpp4 and rpp2 loci. To accomplish this objective, 20 sources of resistance genes mapping out of the loci rpp2 and rpp4 (Laperuta, 2007) were divided into a group with four testers, which were crossed between them, and with the other 16 sources. The parentals and F2 generations from these crosses were inoculated and evaluated in a greenhouse. Each plant was classified according to the reaction of resistance (RB lesion) or susceptibility (TAN lesion). Based on the segregation observed in the F2 generation, it was possible to conclude that among the four sources used as testers, three of them (PI 200487 or "Kinoshita," PI 200526 or "Shira Nui" and GC 84058 18-4) have at least one gene of resistance in the same linkage group (LG), while the other tester (PI203398 or "Abura") has a gene of resistance in an independent locus. Among the other sources tested, three of them (PI 416764 and PI 423966) belong to the group "Kinoshita," three (PI 416810, PI 417421 and PI 398777) belong to the group "Abura", and five (PI 397618TC1, PI 417074, PI 417503, Nova Santa Rosa and Hyuuga) segregated independently in relation to the groups "Kinoshita" and "Abura," which indicates that they have at least one gene of resistance mapping out of the loci 17 tested. The sources GC 84058-21-4 and GC 84051-9-1 didnt segregat in crosses with the tester GC 84058-18-4 and must contain at least one gene next to the LG of "Kinoshita." Three other sources (PI 471904, PI 200455 and PI 417115) not segregated in crosses with "Abura," but it was not possible to conclude about their LG, because the PI 471904 also do not segregate with "Kinoshita, while the PI 200455 do not segregate with the testers PI 200526 and PI 200487 of the group "Kinoshita" and, finally, the PI417115 also do not segregate with PI 200487 of the group "Kinoshita." According to these results, it is possible that we are dealing with a new cluster of genes for disease resistance in LG-N.
Kuwano, Biana Harumi. "Encarquilhamento foliar em soja (Glycine max (L.) Merr.) no Paraná : fatores envolvidos e possíveis causas." Universidade Estadual de Londrina. Centro de Ciências Agrárias. Programa de Pós-Graduação em Agronomia, 2017. http://www.bibliotecadigital.uel.br/document/?code=vtls000218129.
Повний текст джерелаSoybean (Glycine max L. Merr.) producing fields in Paraná State have presented plants with leaf crinkle, similar to symptoms of Mn toxicity, resulting in growth reduction, generally observed in spots. The aim of this work was to analyze soil chemical and microbiological properties related with Mn cycling and investigate if high amounts of this nutrient have caused the "crinkle leaf" in commercial areas of soybean. Soil and plant samples were collected in spots with symptoms and adjacent healthy areas of nine fields in 2012/13 season and three in 2013/14 season. In the shoots, the dry weight and foliar concentrations of P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn were determined. Soil samples were collected at depths of 0-5, 5-10, 10-20 and 20-40 cm, air-dried and analyzed for pH (CaCl2), organic C, available P, Ca, Mg, K, Al, H + Al, Sum of bases, CEC, base saturation, B, Cu, Fe, Mn and Zn concentrations. Microbiological analyzes were carried out in soil samples from 0-5, 5-10 cm and rhizosphere. The number of colony forming units (CFU) of oxidizing and Mn reducing bacteria was evaluated, and the most representative isolates had the 16S rDNA region sequenced for identification. Microbial biomass C (MBC), basal respiration and mycorrhizal colonization were also assessed. Plants with foliar crinkling presented lower shoot dry weight and grain yield. In addition, showed higher foliar concentrations of P, Ca, Mg, Mn and B, and lower concentrations of K. The values of SB, CEC, Ca, Mg and Fe concentrations (0-5 cm) were higher in the soil of plants without symptoms, whereas P and Zn (10-20 cm) were lower. In the 2012/13 season, MBC was significantly higher in the soils of plants without symptoms. The number of CFUs of Mn-oxidizing bacteria was higher in the soil of the symptomless area (0-5 cm, rhizosphere), whereas the number of Mn-reducing bacteria was higher in the area with plants showing symptoms. The largest population of Mn-reducing bacteria in the area with symptoms coincided with slightly higher concentrations of Mn in plant leaves, compared with plants without symptoms. The genus Streptomyces predominated among the Mn-reducing bacteria, while several genera represented the Mn-oxidizing bacteria: Arthrobacter, Streptomyces, Bacillus, Novosphingobium, Agrobacterium, Variovorax, Acinetobacter and Pseudomonas. The P concentrations differed between both soil and plants, while Ca and Mg differed only in the soil, suggesting that the symptom may not be related to a particular nutrient, but may depend on the interaction between several factors. Despite the concentrations of Mn in plants and in the soil are considered high, they could not be considered the sole cause of the problem. A multifactorial interaction probably occurs and must be more deeply studied.
Rosset, Michele. "Hidrólise enzimática de carboidratos de soja [Glycine max (L.) Merrill] e efeitos em tofu tipo silken." Universidade Estadual de Londrina, 2011. http://www.bibliotecadigital.uel.br/document/?code=vtls000168335.
Повний текст джерелаThe addition of Viscozyme L in soy suspension may result in a tofu with characteristics differents from traditional. Due to the presence of the enzyme, cell wall material (polysaccharides) may be partially hydrolyzed to release mono-and oligosaccharides, which will be transferred to the tofu and probably influencing composition and texture. The aim of this work was to study the effects, in silken tofu, of the hydrolysis of soy carbohydrates by the enzyme complex of Viscozyme L. First, this study investigated the enzymatic pre-treatment of soy slurry to optimize conditions of the enzyme action. The optimum temperature of Viscozyme L was 55 oC and it was found that the levels of reducing sugars increased up to 4 times compared to the control. Stachyose was the predominant oligosaccharide in treated tofu 4.58 g/100 g, and of raffinose was 1.22 and 0.75 g/100 g in treated tofu and control, respectively. The glucose level was approximately doubled in the treated tofu (1.66 g/100 g) compared to control (0.74 g/100 g). The treated tofu had a higher amount of phenolic compounds compared to the control, 173 and 161 mg of gallic acid equivalent/100 g of dried tofu, and higher antioxidant activity by ABTS and DPPH test. The total content of isoflavonas (92 mMol/100 g tofu) did not differ between the samples but the treated tofu had a higher concentration of malonyl glycosides and the control of ?-glycosides. The tofus showed sensory differences as the largest soybean odor and less uniform surface (treated tofu), but there was no preference for one sample over the other. The treated tofu had higher amount of glucose and fructose than the control, but there was no observed differences in the taste (acid and bitter) of the samples. This may have occurred because the tofus were coagulated with glucona-delta-lactone, an acid coagulant that may have masked the sweet taste of treated tofu. The optimum conditions of temperature and concentration of Viscozyme L for protein extraction were 60 ° C and 30 FBG (Fungal Beta glucanase), during 30 minutes. Enzimatic pre-treatment for proteins extraction resulted in a yield of 56.27%, higher than the traditional alkaline method, 33.04%; the effect of the pre-treatment temperature was the most important variable. For carbohydrate hydrolysis the optimal conditions of temperature and enzyme concentration were 45° C and 45 g of FBG/10 g defatted soy flour. All tofu samples had a globular microstructure of protein which was integrated into clumps and showed a fibrous three-dimensional network structure, typical of tofu.
Almeida, Adriély Alves de. "Tratamento de sementes como alternativa para o controle de Meloidogyne javanica em soja Glycine max (L.) Merril." Universidade Estadual de Londrina. Centro de Ciências Agrárias. Programa de Pós-Graduação em Agronomia, 2014. http://www.bibliotecadigital.uel.br/document/?code=vtls000196041.
Повний текст джерелаThe seed treatment have been demonstrated good prospects as a complementary measure to control nematodes. Therefore, this study aimed to investigate the effect of products used in the seeds treatment of soybean on root-knot nematode Meloidogyne javanica. To accomplish this, experiments were conducted in vitro and on greenhouse consisted of combinations of treatments: Control (inoculated and non-inoculated, for the test in a greenhouse with nematode and without seed treatment); Abamectin; Tiametoxam; Fludioxonil + Metalaxyl-M + Tiabendazole; Abamectin + Tiametoxam; Abamectin + Fludioxonil + Metalaxyl-M + Tiabendazole; Tiametoxam + Fludioxonil + Metalaxyl -M + Tiabendazole, and Abamectin + Tiametoxam + Fludioxonil + Metalaxyl-M + Tiabendazole. The in vitro assay was performed in order to verify the effect of chemicals on hatching, mobility and mortality of nematode second stage juveniles ( J2 ) . For the experiment in greenhouse, seeds were pretreated by the method of plastic bag with the following combination of treatment. The sowing was performed in glass with a capacity of 3 liters of substrate, leaving one plant per pot. At 15 days after inoculation, the treatment effect on plant growth and nematode penetration was evaluated by th the acid fuchsin method. After 30 and 60 days, were taken measures of plant development again, as well as the number of galls and egg masses, final population, reproduction factor and number of nematodes per gram of root. Treatments containing abamectin were the most effective in reducing the hatching rate of J2, as well as those which had higher immobilization and mortality rates on juveniles, differing from control. At 15 days, there was greater root development, length of shoot and fresh weight of shoots in plants grown from treated seeds. For the number of nematodes/g of root was observed significant difference among plants grown from treated seeds and inoculated control highlighting the treatments containing abamectin, isolated or in combination with other products. After 30 days, it was confirmed the effect of seed treatment on reducing nematode populations with treatment 3 (Abamectin) and 9 (Abamectin + Tiametoxam + Fludioxonil + Metalaxyl -M + Tiabendazole), with smaller values for final population factor, reproduction factor and nematodes/g of root. However, at 60 days, after inoculation, there was no effect of treatments on maintaining low nematode populations. Therefore, from the results obtained it can be concluded that the seeds treatment may be a measure that assists in the nematode integrated management.
Santos, Esmael Lopes dos. "Influência do genótipo sobre as concentrações de proteína e óleo em sementes de soja [Glycine max (L.) Merrill]." Universidade Estadual de Londrina. Centro de Ciências Agrárias. Programa de Pós-Graduação em Agronomia, 2006. http://www.bibliotecadigital.uel.br/document/?code=vtls000117318.
Повний текст джерелаSoybean [Glycine max (L.) Merrill] confirms every year its unparalleled position of most important agricultural Brazilian product as regards the flow of money to the country and the increasing of agro-industry activities and also meat, oil and respective sub products production. In Brazil as well as in the rest of the world, soybean is mainly used as raw material of the grinding industry which produces degummed oil and meal. Soybean oil is the raw material of the food industry for the production of refined oil and its sub products and the meal is mainly used to increase the protein content in animal food. The quality of soybean meal is evaluated by its protein content. Meal resulting from soybean produced in Brazil, specifically in southern region of Brazil, has been presenting a protein content lower than the demands of the market, what results in the product?s devaluation. The concentration of oil and protein in soybean seed is an inherited qualitative trait, but it is greatly influenced by the environment .Under the condition of cultivation in vitro it is difficult to control precisely the supply of carbon and nitrogen destined for the seeds. With the purpose of evaluating soybean?s genotype on the concentration of protein and oil of seeds developed in vivo and in vitro, immature seeds of cultivars CD 202 and CD 206 were removed from the mother-plant in the stage R5 and were cultivated in vitro, in a liquid milieu of cultivation which contained 20, 40 and 60 mM of glutamine. The seeds were incubated in glass flasks and agitated constantly during eight days at 25± 0,2 º C with 204,5 mM sucrose concentration. After that period of incubation it was determined the gain of fresh mass of the seeds. Afterwards it was also determined the oil and protein contents of the seeds developed in vitro and those developed in vivo. The fresh mass gain did not present a significant difference ( P>0,05) in the same cultivar when the glutamine concentration was altered , but between the two cultivars, fresh mass gain showed a significant difference (P<0,05) in the concentration of 40 and 60 mM of glutamine in the cultivar CD 206 as compared to the cultivar CD 202. The protein percentage in the 20 mM glutamine concentration did not present a significant difference (P>0,05) in the cultivar CD 206 cultivated in vivo. Nevertheless the opposite occurs in 40 and 60 mM of glutamine concentrations. Cultivar 202 when cultivated in vitro in 20 mM concentration showed a lower protein percentage than that of the seed cultivated in vivo. However, over 20 mM glutamine concentration, the cultivar CD 202 showed a high percentage of protein, what represents a good response to the increase of glutamine concentration. With an adequate supply of nitrogen for the seeds cultivated in vitro, the genotype did not limit the protein gain. Between the studied cultivars, protein percentage was always higher in the cultivar CD 206. Oil and protein concentrations were inversely related. The genotype has influence on soybean seeds composition, since it was observed that statistical differences in the oil and protein percentage remained unchanged even when a variation in the nitrogen supply to the seeds developed in vitro ocurred.
Santos, Maria Aparecida dos. "Identificação de QTL associados à simbiose entre bradyrhizobium japonicum/B. elkanii e a soja [Glycine max(L.) Merr.]." Universidade Estadual de Londrina. Centro de Ciências Biológicas. Programa de Pós-Graduação em Microbiologia, 2005. http://www.bibliotecadigital.uel.br/document/?code=vtls000108570.
Повний текст джерелаNitrogen (N) demand of soybean [Glycine max (L.) Merrill] can be supplied via biological nitrogen fixation (BNF) through the inoculation with selected Bradyrhizobium japonicum/B. elkanii strains, such that today no N-fertilizer is recommended for the crop in Brazil. However, traits related to BNF have not been lately evaluated in soybean breeding programs, with priority given to yield and resistance to diseases. The objective of this study was the identification of QTL (Quantitative Trait Loci) related to BNF using microsatellites (SSR) markers, in an F2:7 population of 157 Recombinant Imbred Lines (RILs), derived from the cross between parental cultivars with contrasting capacities of BNF, Bossier (high) and Embrapa 20 (medium). Soybean lines were grown under greenhouse conditions for the evaluation of the parameters of plant growth (shoot dry weight, SDW), and nodulation (nodule number, NN; nodule dry weight, NDW and the relation nodule dry weight/nodule number, NDW/NN). All parameters evaluated showed statistical differences (P= 0.05), indicating genetic variability among soybean lines. Sixteen markers located in six out of the twenty soybean linkage groups have been mapped, covering about 5% of the genome (151.6 cM). The regression analysis identified twelve significant associations in four linkage groups (B1, C2, D1b and H): three for shoot weight, four for nodule number, four for nodule weight and three for the medium value of nodule weight. All QTL had minor effects (R2 = 2,5 to 8,0%) similar to previous reports in an F2:3 of BRS 133 (low) x Embrapa 20 (medium) (Nicolás et al., 2005). However, seven QTL were confirmed in both populations, indicating that they might be effective in increasing BNF in soybean breeding programs.
Seibel, Neusa Fátima. "Caracterização, fracionamento e hidrólise enzimática dos componentes do resíduo do processamento da soja [Glycine Max (L.) Merrill], fibras dos cotilédones." Universidade Estadual de Londrina. Centro de Ciências Agrárias. Programa de Pós-Graduação em Ciência de Alimentos, 2006. http://www.bibliotecadigital.uel.br/document/?code=vtls000118533.
Повний текст джерелаSoybean derived ingredients: cotyledon fibers, defatted flour and protein concentrate were evaluated for chemical characteristics and functional properties. Potassium was the macro and iron the micro mineral in highest concentration in all the ingredients. The highest concentration of phytic acid was in the protein concentrate, as well as the highest concentration of isoflavones, however, the fibers had more genistin and daidzein and the total isoflavones in the fibers corresponded to 45% of the amount in the defatted flour. Dietary fibers had the best hydration properties and in properties related to oil emulsification had similar indexes to soybean flour, despite the lower protein concentration. The cell wall soybean cotyledon fiber was fractioned and the major component was hemicellulose, corresponding to 55% on the average. Based on monosaccharide composition of the cell wall fractions there were two groups: (1) cellulose and (2) pectin and hemicelluloses. Protein classification due to solubility in different solvents showed that defatted flour had a major protein fraction extracted with salt solution while the protein concentrate and dietary fibers had higher protein solubility with alkaline solution. The electrophoresis presented the major soybean proteins, ß-conglicinin and glicinin in cotyledon fibers and in protein ingredients. The protein extracted from fibers also reveled bands with molecular weigh next 30KDa, probably cell wall hydroxyproline-rich glycoproteins. After identifying the best conditions, the soybean fibers were hydrolyzed with carbohydrase and protease. The solid fraction of carbohydrase hydrolyzed fiber had higher protein concentration and increased hydrophilic properties, while the soluble fraction had 73% carbohydrates and 50% uronic acids of the initial quantity in samples. The protease hydrolyzed 54% of the total protein in the fiber samples, producing a solid fraction with 76% total dietary fiber. The soluble fraction had peptides of low molecular weigh, lower than 10KDa, and one band of molecular weigh close to 25KDa. The SEM micrographies confirmed the reduced particle in the milled sample in relation to the original sample. The physical structure of the original fiber hydrolyzed with protease had more superficial porosity than the reduced particle fiber. The hydrolyzed samples with carbohydrase presented a more compact structure.
Aoyagi, Luciano Nobuhiro. "Caracterização estrutural e transcricional dos fatores de transcrição R2R3-MYB no genoma da soja (Glycine max) em resposta a doenças." Universidade Estadual de Londrina. Centro de Ciências Exatas. Programa de Pós-Graduação em Biotecnologia, 2013. http://www.bibliotecadigital.uel.br/document/?code=vtls000188152.
Повний текст джерелаSoybean is one of the most important crops for the economy, since it is the main product of the agricultural sector and export of Brazil. Asian soybean rust caused by Phakopsora pachyrhizi, is a limiting factor for the crop expansion and a threat because of its potential yield losses and increase in cultivation costs. The control carried out with chemicals is expensive and cause undesirable impacts to humans and the environment, creating the need to seek for alternatives and equally effective control. A more profound understanding of the mechanisms of defense and resistance of soybean plants, as well as the search for genes associated with these processes represent a path to find ways to overcome the problems caused by FAS and other pathogens. Studies of the interaction of soybeans with FAS indicate that transcription factors (TF) MYB family are differentially expressed in resistant plants. In Arabidopsis, MYBs are extensively studied and have well-defined functions, including control over the defense of the plant. Despite evidence suggest a possible role of these TFs in controlling important processes in soybean, including defense response, there is a lack of knowledge, compared with Arabidopsis. MYB is currently the largest family of FT of G. max, and also in plants, indicating great potential to contain candidates that act to control important processes. Aiming to increase knowledge about these mechanisms, in this work we evaluated the family of MYB transcription factors. GmMYBs class R2R3 were identified from the soybean genome using bioinformatics (SMART, Pfam and MEME), and putative function was determined on the basis of phylogenetic tree construction and classification of subfamilies in GmMYBs using guides (AtMYB) with functions known. All MYB subfamilies of Arabidopsis thaliana and some soybean seen in previous works were formed, as well as new subgroups. The expression and transcriptional profile of GmMYBs R2R3 assays with FAS through analysis in banks of transcritomas (Soybase, LGE-Genosoja, Genevestigator) was performed and associated with determining the putative functions for search potential genes involved in the control mechanism defense. After the selection of target genes, induction by infection with FAS was assessed by quantitative PCR in real time at different times of inoculum (12, 24, 48, 72 and 96 hai) into two treatments (with or without inoculum of spores of P. pachyrhizi). Assays were performed in three genotypes, two resistant PI230970 (resistant) having Rpp2 allele and the allele that has Shiranui Rpp5, and showing lesions of the type RB in response to FAS and Williams 82 (susceptible) having TAN type lesions. Among the genes evaluated, there was especially those related to lignin synthesis with three GmMYBs induced in plant resistance in higher levels and faster, one GmMYB with unknown function and other possibly connected to modulation of responsive genes chitin, both induced by a longer period in the plant resistant.
Godoy, Leandro Pereira de. "Panorama genômico da estirpe semia 5079 (=CPAC 15) de Bradyrhizobium Japonicum, recomendada comercialmente para a cultura da soja (Glycine max (L) merr.)." Universidade Estadual de Londrina. Centro de Ciências Biológicas. Programa de Pós-Graduação em Microbiologia, 2007. http://www.bibliotecadigital.uel.br/document/?code=vtls000125585.
Повний текст джерелаThe biological nitrogen fixation (BNF) process with bacteria belonging to the species Bradyrhizobium japonicum and B. elkanii is crucial for the economical viability of the soybean crop in Brazil. A strain selection program began with the crop expansion in the earlier 1960s, aiming at the identification of bacteria adapted to the local environmental conditions and to the Brazilian cultivars; strain SEMIA 5079 (= CPAC 15) of B. japonicum was then recognized by its efficiency, competitiveness and adaptability to stressful environmental conditions, therefore it has been employed in commercial inoculants since 1992. Few rhizobial genomes were completly sequenced in the last decade, and allowed an impressive increase in the genetic knowledge of these bacteria. In this context, this study aimed at the partial sequencing of the genome of strain SEMIA 5079, searching for genes related to the saprophytic capacity, competitiveness and efficiency of the BNF process. The readings of shotgun libraries built with SEMIA 5079 total DNA resulted in a real covering of 13.17% of the genome, with a total of 1.371 CDSs (coding sequences) annotated, 729 with known functions, 312 classified as conserved hypothetical and 330 as hypothetical. The functional classification of the CDSs in the database COG (Clusters of Orthologous Groups of proteins) identified putative genes in almost all classes. Furthermore, the comparison with strain USDA 110 of B. japonicum, sequenced in 2002 by Japanese researchers, has also demonstrated similar percentages of CDSs classified in the categories of COG, an important indication to support the strategy of partial genome sequencing of a bacterium. In relation to the KEGG (Kyoto Encyclopedia of Genes and Genomes) database, the CDSs of SEMIA 5079 have shown similarity with the sequences of 71 other organisms, but in a higher number with B. japonicum strain USDA 110, and with Bradyrhizobium sp. strains BTAi1 and ORS278. Fourteen transposases were found, most related to insertion sequences, indicating high plasticity of the genome of SEMIA 5079; furthermore, 4.54% of the CDSs were classified as paralog genes, also favoring environmental adaptation. Several CDSs were annotated as genes that codify proteins related to the nodulation, as nodB, nodD2 and nodW genes, and to the BNF, as nifE, fixP, fixQ, and fixR genes, in addition to other proteins involved in the cycle of N. The partial sequencing of the genome of strain SEMIA 5079 has also identified 5.76% of CDSs participating in almost all pathways of xenobiotic biodegradation, and might represent a reservoir of important genes with biotechnological potential.
Marks, Bettina Berquó. "Ação de metabólitos secundários e de inoculantes microbianos na promoção de crescimento de soja (Glycine max (L.) Merr.) e milho (Zea mays L.)." Universidade Estadual de Londrina. Centro de Ciências Biológicas. Programa de Pós-Graduação em Microbiologia, 2013. http://www.bibliotecadigital.uel.br/document/?code=vtls000183388.
Повний текст джерелаWith the current environmental deterioration, caused, among other factors, by the progressive increase of the world population, Brazil stand out as being a storehouse of food worldwide. Among the cultures that play an important role in the Brazilian agricultural scenario we highlight the soybean (Glycine max (L.) Merr.) and maize (Zea mays L.). The substitution of nitrogen fertilizer use by bio-fertilizers and inoculants containing nitrogen-fixing rhizospheric bacteria (rhizobia) and plant-growth promoting rhizobacteria (PGPR) can lower production costs for farmers and reduce environmental pollution by reducing the greenhouse-gas emissions, and contamination of soil and groundwater. The rhizosphere microorganisms known to produce compounds derived from secondary metabolism, which increases their survival and can also promote plant growth. Among these compounds stand out antimicrobials, exopolysaccharides, lipochitooligosaccharides (LCOs) and plant hormones. Despite recent advances in understanding how the chemical signals bacteria-host plant occur, molecules derived from bacterial secondary metabolism are still poorly used to increase agricultural production. This study tested the effects of concentrated secondary metabolites (CSMs) from two strains of rhizobia, Bradyrhizobium japonicum USDA 110 and Rhizobium tropici CIAT 899 at different concentrations, in two important plant-bacteria interactions: B. japonicum-soybean and Azospirillum brasilense-maize. For the soybean, two field and two greenhouse experiments were performed and effects of addition of homologous and heterologous CSM, and of the flavonoid genistein, added the inoculant containing B. japonicum were investigated. For the maize, three field and three greenhouse experiments were performed to verify the effects of the CSM addition, from both strains, to inoculant containing A. brasilense. The following parameters were analysed: dry mass of shoots (DMS), N content in shoot (mgN/g), N accumulation in the shoot (mgN/pl) [in soybean and maize field and greenhouse experiments]; dry mass of root (DMR) [in soybean and maize greenhouse experiments]; grain production (PROD) [in soybean and maize field experiments]; nodule number (NN) and dry mass of nodules (DMN) [in soybean experiments]. For the soybean, compared with the treatment inoculated exclusively with B. japonicum, benefits were achieved with the addition of B. japonicum CSM, and at the concentration of 10-9 M improved dry weight of nodules and grain yield by an average of 4%. In greenhouse experiments, the addition of CSMs did not produce soybean plants with significantly higher values than the other treatments. For the maize field experiments, the best results were obtained with the addition of R. tropici CSM at the concentration of 10-8 M, increasing grain yield by an average of 17% when compared to treatment inoculated + 75% N. In greenhouse experiments, maize plants that have the best parameters were those treated with CSM derived from B. japonicum at a concentration 10-9 M. Benefits might be related to a combination of positive effects attributed to CSMs, including the protective effects of exopolysaccharides, and the growth promoting effects of LCO and plant hormones. The results emphasize the biotechnological potential of using secondary microbial metabolites of rhizobia together with inoculants containing both rhizobia and PGPR to improve plant growth and yield of grain crops.
Kamizake, Neide Kiyoko Kondo. "Efeito do envelhecimento de soja (Glycine max) sobre as propriedades físicas e químicas dos grãos, extratos e tofus e características sensoriais dos tofus." Universidade Estadual de Londrina. Centro de Ciências Agrárias. Programa de Pós-Graduação em Ciência de Alimentos, 2012. http://www.bibliotecadigital.uel.br/document/?code=vtls000182075.
Повний текст джерелаAfter the harvest, the soybeans are often stored in temperature and relative humidity (RH) environment and uncontrolled, resulting in aging and loss of grain quality. Main goals of this study were to investigate the physical and chemical changes in the grains, soy milks and tofus due to the aging of soybean cultivars Coodetec 214 and BRS 267, the effect of aging the grain on coagulant concentration (MgSO4), sensory characteristics (appearance, aroma, flavor and texture) and acceptance of tofu. Soybeans were stored under three conditions: accelerated aging (EA), 30 ºC, 84% RH, nine months; natural aging (EN), room temperature, RH (range in the period = 17.3 at 24.5 ºC and 59 at 93% RH), 18 months and control (C), -20 ºC and 47% RH. Storage in each condition was performed in duplicate. The following evaluations were performed in the samples: scanning electron micrograph (SEM) of the hull and cotyledons, water sorption rate and equilibrium moisture during grain hydration, instrumental color measurement of whole and ground grains and hardness of cooked soybean, proximate composition, minerals (Ca, Mg, P), phytate content, total phenolic, protein solubility and absorption and retention of water by ground grain. The soy milks were obtained by the method semi-kanetsu-shibori and had the color and chemical composition were determined. To prepare tofu, the optimum concentration of coagulant (COC) was measured by the titration method. The tofu was characterized by measuring: color and texture profile, sensory analysis (Quantitative Descriptive Analysis - QDA) employed by 13 trained judges, and acceptance evaluated by hedonic scale and 110 consumers. The data were treated by ANOVA, Tukey test, Pearson correlation analysis (p ≤ 0.05) and Principal Component Analysis. Throughout the storage period AA and NA observed darkening of whole and ground grains, the surfaces of the palisade cells of the cotyledons became rough and wilted, the water sorption rate and hardness of cooked grains increased and equilibrium moisture content decreased, the content of phytate not varied, total phenolic and acidity increased, lipids, pH, water absorption capacity and proteins solubility decreased. The protein electrophoretic patterns changed only for the AA condition affer 8 months. The soy milks of AA and NA conditions were more dark and with lower solids content than control over time. The COC decreased with increasing temperature of coagulation and aging time, and it was positively correlated with total solids content, protein, ash, Ca, Mg and P of aqueous extracts. There was a reduction in yield and color parameters (L* and h°), proteins, lipids, carbohydrates, ash, Ca, Mg, P, brittleness, hardness, elasticity, cohesiveness, gumminess and increased adhesiveness of tofu with the aging time. The QDA indicated that tofus of soybean control characterized by light color, bright and uniform, cohesive appearance, sweet aroma and taste and firm and elastic texture, while the tofus of soybean condition AA (6 months) have gray color with dark spots, appearance rough, fermented aroma and flavor, rancid taste, astringent, bitter, brittle texture, but with adherence in the mouth after chewing. The tofus of soybean condition NA (18 months) showed sensory attributes with intermediate intensities to control and AA. The preference for tofu soybean control was justified by the characteristics of appearance, aroma, flavor and texture.
Roméo, Yves. "Etude des mécanismes de régulation de l'opéron busA qui code un système de transport de la glycine bétaine chez Lactococcus lactis : identification et caractérisation du régulateur transcriptionnel BusR." Toulouse 3, 2003. http://www.theses.fr/2003TOU30043.
Повний текст джерелаRezende, José Marcos [UNESP]. "Ácaros associados a plantas da região Core do Cerrado e sua influência na acarofauna em cultivos de soja (Glycine max (L.) Merril) (Fabaceae)." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/87630.
Повний текст джерелаFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
O Cerrado é um dos principais biomas brasileiros devido à abundância de espécies endêmicas e à extensão territorial. Apresenta várias fitofisionomias, dentre as quais o cerrado sensu stricto. Parte desse bioma tem sido destruída para avanço da agricultura, principalmente para o cultivo de soja (Glycine max (L.) Merril) (Fabaceae), que tornou-se uma das principais atividades da região. Assim, percebe-se que o modelo de agricultura atual não mantém o equilíbrio entre produção agrícola e preservação ambiental. Essa integração passa necessariamente pelo conhecimento sobre as espécies de organismos que habitam as áreas próximas aos cultivos; já estas podem se dispersar para as lavouras e interferir na sua produtividade. Ácaros são importantes neste contexto, principalmente pela existência de espécies-praga e espécies predadoras. Assim, este estudo teve como objetivo conhecer a acarofauna associada a plantas em áreas de cerrado sensu stricto e cultivos de soja na região central do país, além de verificar a influência da fitofisionomia sobre a acarofauna dessa cultura. Para isso, o trabalho foi organizado em três capítulos. No primeiro, foi realizado um levantamento da acarofauna associada a plantas nativas em 10 fragmentos de cerrado sensu stricto através de amotragens qualitativas, além de análise da similaridade e diversidade β para as comunidades de ácaros entre indivíduos de plantas hospedeiras localizados em diferentes áreas. Foram encontradas 123 espécies de ácaros, pertencentes a 20 famílias. Os testes de similaridade e diversidade β demonstraram que uma mesma espécie de planta apresentou diferentes comunidades de ácaros, conforme o fragmento analisado. Isso sugere que somente a conservação de várias áreas de cerrado sensu stricto ao longo do bioma pode manter preservada sua acarofauna plantícola. No capítulo dois, foi realizado...
The Cerrado is one of the major biomes due to abundance of endemic species and territorial dimension. It presents various vegetation types, among which there is the cerrado sensu stricto. Part of this biome has been lost to agricultural encroachment, mainly for the cultivation of soybean (Glycine max (L.) Merril) (Fabaceae), that became one of main activities in the region. Thus, it realized that this model does not maintain a balance between agricultural production and environmental preservation. This integration necessarily involves knowledge about the species of organisms that inhabit the areas near the crops, since they can disperse into crops, and interfere with their productivity. Mites are important in this context mainly by the existence of pest species and predators. Therefore, this study aimed to know the mites associated with plants in the cerrado sensu stricto and soybean crops in the central region of the country, and to investigate the influence of this phytophysiognomy on the mites in soybeans. For this, the work was organized into three chapters. At first, a survey was made of the mite fauna associated with native plants of the 10 fragments of Cerrado through qualitative samplings, and analysis of similarity and β diversity to mite communities among individuals of host plants located in different areas. 123 species of mites were found, belonging to 20 families. Tests for similarity and β diversity demonstrated that a single plant species may have different plant communities of mites, according to fragment. This suggests that only the preservation of various areas of Cerrado along the biome can keep its mite fauna preserved. In chapter two, a survey was made of mite species associated with 10 soybean fields in the middle region of the Cerrado by quantitative and qualitative samplings, and also ecological analysis. 30 species of mites were found, belonging... (Complete abstract click electronic access below)
Rezende, José Marcos. "Ácaros associados a plantas da região "Core" do Cerrado e sua influência na acarofauna em cultivos de soja (Glycine max (L.) Merril) (Fabaceae) /." São José do Rio Preto : [s.n.], 2011. http://hdl.handle.net/11449/87630.
Повний текст джерелаBanca: Carlos H. W. Flechtmann
Banca: Samuel Roggia
Resumo: O Cerrado é um dos principais biomas brasileiros devido à abundância de espécies endêmicas e à extensão territorial. Apresenta várias fitofisionomias, dentre as quais o cerrado sensu stricto. Parte desse bioma tem sido destruída para avanço da agricultura, principalmente para o cultivo de soja (Glycine max (L.) Merril) (Fabaceae), que tornou-se uma das principais atividades da região. Assim, percebe-se que o modelo de agricultura atual não mantém o equilíbrio entre produção agrícola e preservação ambiental. Essa integração passa necessariamente pelo conhecimento sobre as espécies de organismos que habitam as áreas próximas aos cultivos; já estas podem se dispersar para as lavouras e interferir na sua produtividade. Ácaros são importantes neste contexto, principalmente pela existência de espécies-praga e espécies predadoras. Assim, este estudo teve como objetivo conhecer a acarofauna associada a plantas em áreas de cerrado sensu stricto e cultivos de soja na região central do país, além de verificar a influência da fitofisionomia sobre a acarofauna dessa cultura. Para isso, o trabalho foi organizado em três capítulos. No primeiro, foi realizado um levantamento da acarofauna associada a plantas nativas em 10 fragmentos de cerrado sensu stricto através de amotragens qualitativas, além de análise da similaridade e diversidade β para as comunidades de ácaros entre indivíduos de plantas hospedeiras localizados em diferentes áreas. Foram encontradas 123 espécies de ácaros, pertencentes a 20 famílias. Os testes de similaridade e diversidade β demonstraram que uma mesma espécie de planta apresentou diferentes comunidades de ácaros, conforme o fragmento analisado. Isso sugere que somente a conservação de várias áreas de cerrado sensu stricto ao longo do bioma pode manter preservada sua acarofauna plantícola. No capítulo dois, foi realizado... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The Cerrado is one of the major biomes due to abundance of endemic species and territorial dimension. It presents various vegetation types, among which there is the cerrado sensu stricto. Part of this biome has been lost to agricultural encroachment, mainly for the cultivation of soybean (Glycine max (L.) Merril) (Fabaceae), that became one of main activities in the region. Thus, it realized that this model does not maintain a balance between agricultural production and environmental preservation. This integration necessarily involves knowledge about the species of organisms that inhabit the areas near the crops, since they can disperse into crops, and interfere with their productivity. Mites are important in this context mainly by the existence of pest species and predators. Therefore, this study aimed to know the mites associated with plants in the cerrado sensu stricto and soybean crops in the central region of the country, and to investigate the influence of this phytophysiognomy on the mites in soybeans. For this, the work was organized into three chapters. At first, a survey was made of the mite fauna associated with native plants of the 10 fragments of Cerrado through qualitative samplings, and analysis of similarity and β diversity to mite communities among individuals of host plants located in different areas. 123 species of mites were found, belonging to 20 families. Tests for similarity and β diversity demonstrated that a single plant species may have different plant communities of mites, according to fragment. This suggests that only the preservation of various areas of Cerrado along the biome can keep its mite fauna preserved. In chapter two, a survey was made of mite species associated with 10 soybean fields in the middle region of the Cerrado by quantitative and qualitative samplings, and also ecological analysis. 30 species of mites were found, belonging... (Complete abstract click electronic access below)
Mestre
Pivetta, Laércio Augusto [UNESP]. "Avaliação do sistema radicular da soja sob sistemas de manejo do solo." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/86414.
Повний текст джерелаCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
A avaliação do sistema radicular de uma cultura é fundamental para o desenvolvimento de práticas agrícolas que visam a otimização da produtividade. Além disso, a distribuição das raízes no perfil do solo não necessariamente condiz com a atividade radicular, que é mais importante no tocante a absorção de água e nutrientes. O objetivo do experimento foi avaliar o efeito de sistemas de manejo sobre o sistema radicular e a produtividade da soja e comparar as metodologias do trado e do elemento não marcado (rubídio), na análise radicular. As avaliações foram realizadas na safra agrícola 2009/2010, em um experimento instalado desde 2003. O delineamento experimental utilizado foi de blocos ao acaso, em esquema de parcelas subdivididas, com quatro repetições. As parcelas foram constituídas pelas culturas de inverno triticale (X Triticosecale Wittmack) e girassol (Helianthus annuus L.), e as subparcelas pelos manejos de primavera envolvendo milheto (Pennisetum glaucum L.), sorgo forrageiro (Sorghum bicolor (L.) Moench) e crotalária júncea (Crotalaria juncea L.), além da escarificação, realizada em 2003 e 2009. Na safra de verão foi cultivada a soja, em todas as subparcelas. As avaliações realizadas foram: densidade de comprimento, área superficial, massa de matéria seca (trado) e atividade radicular (rubídio) da soja, nas distâncias 0,00; 0,11 e 0,22 m da planta de referência, no estádio R4 da cultura, além da produtividade de grãos. Pelo método do trado foram amostradas as camadas 0,00-0,05, 0,05-0,10, 0,10-0,20, 0,20-0,40 e 0,40-0,60 m. O marcador para a atividade radicular, nitrato de rubídio (RbNO3), foi aplicado nas profundidades 0,05, 0,10, 0,20, 0,40 e 0,60 m. A produtividade da soja não foi influenciada pelas culturas de inverno ou manejos de primavera. As culturas de inverno não se diferenciaram em nenhuma das variáveis...
The knowledge of root distribution as affected by cropping systems and its role in nutrient acquisition by plants is important in planning crop rotations and soil management that optimize crops yield. Furthermore, root distribution in the soil profile does not necessarily matches root activity, which is more important in water and nutrient acquisition. The objective of this research was to assess the effects of managements systems on soybean root distribution and yields, and compare a direct method (soil core sampling) with an indirect method (with rubidium) in evaluating the root system. Evaluations were carried out in season 2010, in an experiment that has been running since 2003. The experimental design is a complete randomized block with a split-plot arrangement of treatments with four replicates. Plots consist in fall-winter crops triticale (X Triticosecale Wittmack) and sunflower (Helianthus annuus L.), and the subplots in spring crops pearl millet (Pennisetum glaucum L.), forage sorghum (Sorghum bicolor (L.) Moench) and sunn hemp (Crotalaria juncea L.), besides chisel tillage in 2003 and 2009. In summer, soybean (Glycine max L. (Merril) is sowed in all subplots. The evaluations carried out were: soybean root length, surface, dry matter (soil core sampling), activity (rubidium), at 0.0, 0.11 and 0.22 m from the reference plant, at R4, besides soybean yield. The samples by soil core sampling method were taken at layers 0.00- 0.05, 0.05-0.10, 0.10-0.20, 0.20-0.40 and 0.40-0.60m. The rubidium nitrate was applied at depths 0.05, 0.10, 0.20, 0.40 and 0.60m. Soybean yield was not affected by winter crops or spring managements. Winter crops did not differ in any variable. Among spring managements, the chisel tillage and sunn hemp resulted less soybean root length. The chisel tillage resulted highest soybean root activity at depth 0.10 m, however... (Complete abstract click electronic access below)
Pivetta, Laercio Augusto 1986. "Avaliação do sistema radicular da soja sob sistemas de manejo do solo /." Botucatu : [s.n.], 2011. http://hdl.handle.net/11449/86414.
Повний текст джерелаBanca: Juliano Carlos Calonego
Banca: José Salvador Simoneti Foloni
Resumo: A avaliação do sistema radicular de uma cultura é fundamental para o desenvolvimento de práticas agrícolas que visam a otimização da produtividade. Além disso, a distribuição das raízes no perfil do solo não necessariamente condiz com a atividade radicular, que é mais importante no tocante a absorção de água e nutrientes. O objetivo do experimento foi avaliar o efeito de sistemas de manejo sobre o sistema radicular e a produtividade da soja e comparar as metodologias do trado e do elemento não marcado (rubídio), na análise radicular. As avaliações foram realizadas na safra agrícola 2009/2010, em um experimento instalado desde 2003. O delineamento experimental utilizado foi de blocos ao acaso, em esquema de parcelas subdivididas, com quatro repetições. As parcelas foram constituídas pelas culturas de inverno triticale (X Triticosecale Wittmack) e girassol (Helianthus annuus L.), e as subparcelas pelos manejos de primavera envolvendo milheto (Pennisetum glaucum L.), sorgo forrageiro (Sorghum bicolor (L.) Moench) e crotalária júncea (Crotalaria juncea L.), além da escarificação, realizada em 2003 e 2009. Na safra de verão foi cultivada a soja, em todas as subparcelas. As avaliações realizadas foram: densidade de comprimento, área superficial, massa de matéria seca (trado) e atividade radicular (rubídio) da soja, nas distâncias 0,00; 0,11 e 0,22 m da planta de referência, no estádio R4 da cultura, além da produtividade de grãos. Pelo método do trado foram amostradas as camadas 0,00-0,05, 0,05-0,10, 0,10-0,20, 0,20-0,40 e 0,40-0,60 m. O marcador para a atividade radicular, nitrato de rubídio (RbNO3), foi aplicado nas profundidades 0,05, 0,10, 0,20, 0,40 e 0,60 m. A produtividade da soja não foi influenciada pelas culturas de inverno ou manejos de primavera. As culturas de inverno não se diferenciaram em nenhuma das variáveis... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The knowledge of root distribution as affected by cropping systems and its role in nutrient acquisition by plants is important in planning crop rotations and soil management that optimize crops yield. Furthermore, root distribution in the soil profile does not necessarily matches root activity, which is more important in water and nutrient acquisition. The objective of this research was to assess the effects of managements systems on soybean root distribution and yields, and compare a direct method (soil core sampling) with an indirect method (with rubidium) in evaluating the root system. Evaluations were carried out in season 2010, in an experiment that has been running since 2003. The experimental design is a complete randomized block with a split-plot arrangement of treatments with four replicates. Plots consist in fall-winter crops triticale (X Triticosecale Wittmack) and sunflower (Helianthus annuus L.), and the subplots in spring crops pearl millet (Pennisetum glaucum L.), forage sorghum (Sorghum bicolor (L.) Moench) and sunn hemp (Crotalaria juncea L.), besides chisel tillage in 2003 and 2009. In summer, soybean (Glycine max L. (Merril) is sowed in all subplots. The evaluations carried out were: soybean root length, surface, dry matter (soil core sampling), activity (rubidium), at 0.0, 0.11 and 0.22 m from the reference plant, at R4, besides soybean yield. The samples by soil core sampling method were taken at layers 0.00- 0.05, 0.05-0.10, 0.10-0.20, 0.20-0.40 and 0.40-0.60m. The rubidium nitrate was applied at depths 0.05, 0.10, 0.20, 0.40 and 0.60m. Soybean yield was not affected by winter crops or spring managements. Winter crops did not differ in any variable. Among spring managements, the chisel tillage and sunn hemp resulted less soybean root length. The chisel tillage resulted highest soybean root activity at depth 0.10 m, however... (Complete abstract click electronic access below)
Mestre
Both, Peter. "Etude structure-fonction d'une fucosyltransférase (FucTA) de Arabidopsis thaliana." Phd thesis, Université Joseph Fourier (Grenoble), 2009. http://tel.archives-ouvertes.fr/tel-00449431.
Повний текст джерелаNisha, G. J. "Molecular Characterization of Mannose Binding Lectins." Thesis, 2018. https://etd.iisc.ac.in/handle/2005/4628.
Повний текст джерелаLin, Chia-Wei, and 林家緯. "Convenient Synthesis of a N-Glycan Core Trisaccharide by the Assistance of a Novel Fluorous Tag." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/31564283620035315308.
Повний текст джерелаPark, Chaeho. "The characterization of a novel human core-specific lysosomal [alpha]1-6mannosidase involved in N-glycan catabolism." 2005. http://purl.galileo.usg.edu/uga%5Fetd/park%5Fchaeho%5F200508%5Fphd.
Повний текст джерелаDirected by Kelley W. Moremen. Includes an article submitted to Journal of biological chemistry. For abstract see http://getd.galib.uga.edu/public/park%5chaeho%5F200508%5Fphd/park%5Fchaeho%5F200508%5Fphd.pdf. Includes bibliographical references.
"Identificação de QTL associados à simbiose entre bradyrhizobium japonicum/B. elkanii e a soja [Glycine max(L.) Merr.]." Tese, Biblioteca Digital da Universidade Estadual de Londrina, 2005. http://bibliotecadigital.uel.br/document/?code=vtls000108570.
Повний текст джерела"Transporte de nitrogenio e metabolismo da aspargina em soja (Glycine Max L.) sob deficiencia na assimilação do nitrogenio." Tese, Biblioteca Digital da Unicamp, 2002. http://libdigi.unicamp.br/document/?code=vtls000248370.
Повний текст джерела"Caracterização, fracionamento e hidrólise enzimática dos componentes do resíduo do processamento da soja [Glycine Max (L.) Merrill], fibras dos cotilédones." Tese, Biblioteca Digital da Universidade Estadual de Londrina, 2006. http://bibliotecadigital.uel.br/document/?code=vtls000118533.
Повний текст джерела"Transporte e metabolismo de glutamina em soja [Glycine max (L.) Merril] : uma relação estreita com a fixação simbiotica do nitrogenio atmosferico." Tese, Biblioteca Digital da Unicamp, 2002. http://libdigi.unicamp.br/document/?code=vtls000257451.
Повний текст джерела"Papel da redutase do nitrato em soja [Glycine max(L.) Merr. Cv IAC-17] sob condições de hipoxia do sistema radicular." Tese, Biblioteca Digital da Unicamp, 2005. http://libdigi.unicamp.br/document/?code=vtls000365798.
Повний текст джерела"Metabolismo de nitrogenio em plantas de soja [Glycine max (L.) Merr. cv IAC-17] submetidas a deficiencia de O2 no sistema radicular." Tese, Biblioteca Digital da Unicamp, 2001. http://libdigi.unicamp.br/document/?code=vtls000219169.
Повний текст джерела"Modelos numericos para a descrição do crescimento da planta de soja (Glycine max L. Merril, cultivar IAC-15) em condições saxonais diferenciadas." Tese, Biblioteca Digital da Unicamp, 2021. http://libdigi.unicamp.br/document/?code=vtls000065423.
Повний текст джерела"Efeito de fontes de nitrogenio e enxofre na sintese das proteinas de reserva de sementes de soja (Glycine max [L] Merril) cultivadas "In Vitro"." Tese, Biblioteca Digital da Unicamp, 1994. http://libdigi.unicamp.br/document/?code=vtls000075134.
Повний текст джерела"Seleção de linhagens e obtenção de hibridos de Saccharomyces cerevisiae Meyen com respectivas de utilização na indução de resistencia a doenças em soja (Glycine max (L.) Merril)." Tese, Biblioteca Digital da Unicamp, 1993. http://libdigi.unicamp.br/document/?code=vtls000069987.
Повний текст джерелаVIJAY, DATTARAM PAWAR. "Diethylene glycol ether as triply branched dendritic anchors to CdSe/ZnS core/shell type nanoparticles: potential hydrophilic fluorescent probes." 2005. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0021-2004200716205387.
Повний текст джерелаJanvier, Florence. "Preparation, characterization, and rheological properties of star-shaped poly(ethylene glycol) with a cholane core and study of its effect on red blood cell aggregation." Thèse, 2009. http://hdl.handle.net/1866/7822.
Повний текст джерела