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Статті в журналах з теми "Glycan code"
Jones, Christopher J., and Cynthia K. Larive. "Cracking the glycan sequence code." Nature Chemical Biology 7, no. 11 (October 18, 2011): 758–59. http://dx.doi.org/10.1038/nchembio.696.
Повний текст джерелаKaltner, Herbert, José Abad-Rodríguez, Anthony P. Corfield, Jürgen Kopitz, and Hans-Joachim Gabius. "The sugar code: letters and vocabulary, writers, editors and readers and biosignificance of functional glycan–lectin pairing." Biochemical Journal 476, no. 18 (September 24, 2019): 2623–55. http://dx.doi.org/10.1042/bcj20170853.
Повний текст джерелаKlein, Joshua, Luis Carvalho, and Joseph Zaia. "Application of network smoothing to glycan LC-MS profiling." Bioinformatics 34, no. 20 (May 22, 2018): 3511–18. http://dx.doi.org/10.1093/bioinformatics/bty397.
Повний текст джерелаKellman, Benjamin P., Yujie Zhang, Emma Logomasini, Eric Meinhardt, Karla P. Godinez-Macias, Austin W. T. Chiang, James T. Sorrentino, et al. "A consensus-based and readable extension of Linear Code for Reaction Rules (LiCoRR)." Beilstein Journal of Organic Chemistry 16 (October 27, 2020): 2645–62. http://dx.doi.org/10.3762/bjoc.16.215.
Повний текст джерелаGabius, Hans-Joachim. "Glycans: bioactive signals decoded by lectins." Biochemical Society Transactions 36, no. 6 (November 19, 2008): 1491–96. http://dx.doi.org/10.1042/bst0361491.
Повний текст джерелаAlocci, Davide, Pavla Suchánková, Renaud Costa, Nicolas Hory, Julien Mariethoz, Radka Vařeková, Philip Toukach, and Frédérique Lisacek. "SugarSketcher: Quick and Intuitive Online Glycan Drawing." Molecules 23, no. 12 (December 5, 2018): 3206. http://dx.doi.org/10.3390/molecules23123206.
Повний текст джерелаDumych, Tetiana, Clarisse Bridot, Sébastien Gouin, Marc Lensink, Solomiya Paryzhak, Sabine Szunerits, Ralf Blossey, Rostyslav Bilyy, Julie Bouckaert, and Eva-Maria Krammer. "A Novel Integrated Way for Deciphering the Glycan Code for the FimH Lectin." Molecules 23, no. 11 (October 28, 2018): 2794. http://dx.doi.org/10.3390/molecules23112794.
Повний текст джерелаLopes, Nuno, Viviana G. Correia, Angelina S. Palma, and Catarina Brito. "Cracking the Breast Cancer Glyco-Code through Glycan-Lectin Interactions: Targeting Immunosuppressive Macrophages." International Journal of Molecular Sciences 22, no. 4 (February 17, 2021): 1972. http://dx.doi.org/10.3390/ijms22041972.
Повний текст джерелаKaltner, Herbert, and Hans-Joachim Gabius. "Sensing Glycans as Biochemical Messages by Tissue Lectins: The Sugar Code at Work in Vascular Biology." Thrombosis and Haemostasis 119, no. 04 (January 8, 2019): 517–33. http://dx.doi.org/10.1055/s-0038-1676968.
Повний текст джерелаYusufi, Faraaz Noor Khan, Wonjun Park, May May Lee, and Dong-Yup Lee. "An alpha-numeric code for representing N-linked glycan structures in secreted glycoproteins." Bioprocess and Biosystems Engineering 32, no. 1 (May 6, 2008): 97–107. http://dx.doi.org/10.1007/s00449-008-0226-4.
Повний текст джерелаДисертації з теми "Glycan code"
Lopes, Valéria Stefania. "Caracterização da família de genes HSP20 em Glycine max." Universidade Estadual de Londrina, EMBRAPA. Centro de Ciências Exatas. Programa de Pós-Graduação em Biotecnologia, 2012. http://www.bibliotecadigital.uel.br/document/?code=vtls000175506.
Повний текст джерелаThe small heat shock proteins (HSP20) are often associated in plant stress response caused by abiotic factors and, more recently, have also been associated with response to biotic stresses. The Hsp20 genes represent, in plants, the most abundant class among the heat shock proteins, but little is known about this gene family in soybean. Due their apparent multifunctionality, these proteins are promising targets to the crop varieties development for better conditions adapted to biotic and abiotic stresses, even when they are combined. Thus, the present study conducted an in silico molecular characterization of regulatory and coding regions of HSP20 genes from soybean, focus in its genome distribution, subcellular localization, division into subfamilies, secondary structure and regulation under biotic and abiotic stresses, besides the identification patterns to cis elements potentially involved in the response to nematodes. After the exploration of Hsp20 genes annotation in soybean genome databases, 76 gene models were obtained. After in silico analysis, just 52 gene models were part of the GmHsp20 potencial candidates due to their structural characteristics of cis elements and expression profile. In addition, based on in vivo analysis, 45 soybean Hsp20 genes were identified, distributed in 11 subfamilies, for which is possible to observe a specific secondary structure for each one. Among the 45 GmHsp20 genes heat stress responsives, 5 genes were cold stress responsive and other five were nematode infection by M. javanica responsive. Moreover, two genes were observed being responsive to biotic stress, but they weren't responsive to thermal shock. Operational Models of Hsp20 promoters were obtained to responsive genes to each stress condition examined in this study. Among the identified cis elements in Hsp20 soybean genes that were responsive to M. javanica infection were W box, CAAT box, ABRE and MYB, besides the HSE / Heat element. Promoters responsive to biotic stress in soybean follows composition and distribution standards of cis elements, as described in the literature to be related to this type of stress. These results, such as responsive genes and promoters to many different stresses, can assist in generation of expression directed technologies even more advanced and new soybean genotypes more adapted to under combined stress conditions.
Rachid, Breno Francovig. "Identificação de novos locos de resistência à ferrugem asiática (Phakopsora pachyrhizi) em soja (Glycine max)." Universidade Estadual de Londrina. IAPAR. EMBRAPA. Centro de Ciências Biológicas. Programa de Pós-Graduação em Genética e Biologia Molecular, 2008. http://www.bibliotecadigital.uel.br/document/?code=vtls000154641.
Повний текст джерелаIn Brazil, the soybean rust, caused by Phakopsora pachyrhizi, has caused yield losses and increased the cost of production by the intensive use of fungicides in soybean fields. The use of resistant varieties is an important tool to control the disease. Currently five different loci have been reported containing genes for resistance to disease, called rpp1 to rpp5. A new race of the fungus broke the resistance conferred by some genes present in the loci rpp1 and rpp3 in Brazil. This study aimed to perform allelism tests between sources of resistance identified in the germplasm bank of Embrapa Soybean, whose genes do not belong to rpp4 and rpp2 loci. To accomplish this objective, 20 sources of resistance genes mapping out of the loci rpp2 and rpp4 (Laperuta, 2007) were divided into a group with four testers, which were crossed between them, and with the other 16 sources. The parentals and F2 generations from these crosses were inoculated and evaluated in a greenhouse. Each plant was classified according to the reaction of resistance (RB lesion) or susceptibility (TAN lesion). Based on the segregation observed in the F2 generation, it was possible to conclude that among the four sources used as testers, three of them (PI 200487 or "Kinoshita," PI 200526 or "Shira Nui" and GC 84058 18-4) have at least one gene of resistance in the same linkage group (LG), while the other tester (PI203398 or "Abura") has a gene of resistance in an independent locus. Among the other sources tested, three of them (PI 416764 and PI 423966) belong to the group "Kinoshita," three (PI 416810, PI 417421 and PI 398777) belong to the group "Abura", and five (PI 397618TC1, PI 417074, PI 417503, Nova Santa Rosa and Hyuuga) segregated independently in relation to the groups "Kinoshita" and "Abura," which indicates that they have at least one gene of resistance mapping out of the loci 17 tested. The sources GC 84058-21-4 and GC 84051-9-1 didnt segregat in crosses with the tester GC 84058-18-4 and must contain at least one gene next to the LG of "Kinoshita." Three other sources (PI 471904, PI 200455 and PI 417115) not segregated in crosses with "Abura," but it was not possible to conclude about their LG, because the PI 471904 also do not segregate with "Kinoshita, while the PI 200455 do not segregate with the testers PI 200526 and PI 200487 of the group "Kinoshita" and, finally, the PI417115 also do not segregate with PI 200487 of the group "Kinoshita." According to these results, it is possible that we are dealing with a new cluster of genes for disease resistance in LG-N.
Kuwano, Biana Harumi. "Encarquilhamento foliar em soja (Glycine max (L.) Merr.) no Paraná : fatores envolvidos e possíveis causas." Universidade Estadual de Londrina. Centro de Ciências Agrárias. Programa de Pós-Graduação em Agronomia, 2017. http://www.bibliotecadigital.uel.br/document/?code=vtls000218129.
Повний текст джерелаSoybean (Glycine max L. Merr.) producing fields in Paraná State have presented plants with leaf crinkle, similar to symptoms of Mn toxicity, resulting in growth reduction, generally observed in spots. The aim of this work was to analyze soil chemical and microbiological properties related with Mn cycling and investigate if high amounts of this nutrient have caused the "crinkle leaf" in commercial areas of soybean. Soil and plant samples were collected in spots with symptoms and adjacent healthy areas of nine fields in 2012/13 season and three in 2013/14 season. In the shoots, the dry weight and foliar concentrations of P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn were determined. Soil samples were collected at depths of 0-5, 5-10, 10-20 and 20-40 cm, air-dried and analyzed for pH (CaCl2), organic C, available P, Ca, Mg, K, Al, H + Al, Sum of bases, CEC, base saturation, B, Cu, Fe, Mn and Zn concentrations. Microbiological analyzes were carried out in soil samples from 0-5, 5-10 cm and rhizosphere. The number of colony forming units (CFU) of oxidizing and Mn reducing bacteria was evaluated, and the most representative isolates had the 16S rDNA region sequenced for identification. Microbial biomass C (MBC), basal respiration and mycorrhizal colonization were also assessed. Plants with foliar crinkling presented lower shoot dry weight and grain yield. In addition, showed higher foliar concentrations of P, Ca, Mg, Mn and B, and lower concentrations of K. The values of SB, CEC, Ca, Mg and Fe concentrations (0-5 cm) were higher in the soil of plants without symptoms, whereas P and Zn (10-20 cm) were lower. In the 2012/13 season, MBC was significantly higher in the soils of plants without symptoms. The number of CFUs of Mn-oxidizing bacteria was higher in the soil of the symptomless area (0-5 cm, rhizosphere), whereas the number of Mn-reducing bacteria was higher in the area with plants showing symptoms. The largest population of Mn-reducing bacteria in the area with symptoms coincided with slightly higher concentrations of Mn in plant leaves, compared with plants without symptoms. The genus Streptomyces predominated among the Mn-reducing bacteria, while several genera represented the Mn-oxidizing bacteria: Arthrobacter, Streptomyces, Bacillus, Novosphingobium, Agrobacterium, Variovorax, Acinetobacter and Pseudomonas. The P concentrations differed between both soil and plants, while Ca and Mg differed only in the soil, suggesting that the symptom may not be related to a particular nutrient, but may depend on the interaction between several factors. Despite the concentrations of Mn in plants and in the soil are considered high, they could not be considered the sole cause of the problem. A multifactorial interaction probably occurs and must be more deeply studied.
Rosset, Michele. "Hidrólise enzimática de carboidratos de soja [Glycine max (L.) Merrill] e efeitos em tofu tipo silken." Universidade Estadual de Londrina, 2011. http://www.bibliotecadigital.uel.br/document/?code=vtls000168335.
Повний текст джерелаThe addition of Viscozyme L in soy suspension may result in a tofu with characteristics differents from traditional. Due to the presence of the enzyme, cell wall material (polysaccharides) may be partially hydrolyzed to release mono-and oligosaccharides, which will be transferred to the tofu and probably influencing composition and texture. The aim of this work was to study the effects, in silken tofu, of the hydrolysis of soy carbohydrates by the enzyme complex of Viscozyme L. First, this study investigated the enzymatic pre-treatment of soy slurry to optimize conditions of the enzyme action. The optimum temperature of Viscozyme L was 55 oC and it was found that the levels of reducing sugars increased up to 4 times compared to the control. Stachyose was the predominant oligosaccharide in treated tofu 4.58 g/100 g, and of raffinose was 1.22 and 0.75 g/100 g in treated tofu and control, respectively. The glucose level was approximately doubled in the treated tofu (1.66 g/100 g) compared to control (0.74 g/100 g). The treated tofu had a higher amount of phenolic compounds compared to the control, 173 and 161 mg of gallic acid equivalent/100 g of dried tofu, and higher antioxidant activity by ABTS and DPPH test. The total content of isoflavonas (92 mMol/100 g tofu) did not differ between the samples but the treated tofu had a higher concentration of malonyl glycosides and the control of ?-glycosides. The tofus showed sensory differences as the largest soybean odor and less uniform surface (treated tofu), but there was no preference for one sample over the other. The treated tofu had higher amount of glucose and fructose than the control, but there was no observed differences in the taste (acid and bitter) of the samples. This may have occurred because the tofus were coagulated with glucona-delta-lactone, an acid coagulant that may have masked the sweet taste of treated tofu. The optimum conditions of temperature and concentration of Viscozyme L for protein extraction were 60 ° C and 30 FBG (Fungal Beta glucanase), during 30 minutes. Enzimatic pre-treatment for proteins extraction resulted in a yield of 56.27%, higher than the traditional alkaline method, 33.04%; the effect of the pre-treatment temperature was the most important variable. For carbohydrate hydrolysis the optimal conditions of temperature and enzyme concentration were 45° C and 45 g of FBG/10 g defatted soy flour. All tofu samples had a globular microstructure of protein which was integrated into clumps and showed a fibrous three-dimensional network structure, typical of tofu.
Almeida, Adriély Alves de. "Tratamento de sementes como alternativa para o controle de Meloidogyne javanica em soja Glycine max (L.) Merril." Universidade Estadual de Londrina. Centro de Ciências Agrárias. Programa de Pós-Graduação em Agronomia, 2014. http://www.bibliotecadigital.uel.br/document/?code=vtls000196041.
Повний текст джерелаThe seed treatment have been demonstrated good prospects as a complementary measure to control nematodes. Therefore, this study aimed to investigate the effect of products used in the seeds treatment of soybean on root-knot nematode Meloidogyne javanica. To accomplish this, experiments were conducted in vitro and on greenhouse consisted of combinations of treatments: Control (inoculated and non-inoculated, for the test in a greenhouse with nematode and without seed treatment); Abamectin; Tiametoxam; Fludioxonil + Metalaxyl-M + Tiabendazole; Abamectin + Tiametoxam; Abamectin + Fludioxonil + Metalaxyl-M + Tiabendazole; Tiametoxam + Fludioxonil + Metalaxyl -M + Tiabendazole, and Abamectin + Tiametoxam + Fludioxonil + Metalaxyl-M + Tiabendazole. The in vitro assay was performed in order to verify the effect of chemicals on hatching, mobility and mortality of nematode second stage juveniles ( J2 ) . For the experiment in greenhouse, seeds were pretreated by the method of plastic bag with the following combination of treatment. The sowing was performed in glass with a capacity of 3 liters of substrate, leaving one plant per pot. At 15 days after inoculation, the treatment effect on plant growth and nematode penetration was evaluated by th the acid fuchsin method. After 30 and 60 days, were taken measures of plant development again, as well as the number of galls and egg masses, final population, reproduction factor and number of nematodes per gram of root. Treatments containing abamectin were the most effective in reducing the hatching rate of J2, as well as those which had higher immobilization and mortality rates on juveniles, differing from control. At 15 days, there was greater root development, length of shoot and fresh weight of shoots in plants grown from treated seeds. For the number of nematodes/g of root was observed significant difference among plants grown from treated seeds and inoculated control highlighting the treatments containing abamectin, isolated or in combination with other products. After 30 days, it was confirmed the effect of seed treatment on reducing nematode populations with treatment 3 (Abamectin) and 9 (Abamectin + Tiametoxam + Fludioxonil + Metalaxyl -M + Tiabendazole), with smaller values for final population factor, reproduction factor and nematodes/g of root. However, at 60 days, after inoculation, there was no effect of treatments on maintaining low nematode populations. Therefore, from the results obtained it can be concluded that the seeds treatment may be a measure that assists in the nematode integrated management.
Santos, Esmael Lopes dos. "Influência do genótipo sobre as concentrações de proteína e óleo em sementes de soja [Glycine max (L.) Merrill]." Universidade Estadual de Londrina. Centro de Ciências Agrárias. Programa de Pós-Graduação em Agronomia, 2006. http://www.bibliotecadigital.uel.br/document/?code=vtls000117318.
Повний текст джерелаSoybean [Glycine max (L.) Merrill] confirms every year its unparalleled position of most important agricultural Brazilian product as regards the flow of money to the country and the increasing of agro-industry activities and also meat, oil and respective sub products production. In Brazil as well as in the rest of the world, soybean is mainly used as raw material of the grinding industry which produces degummed oil and meal. Soybean oil is the raw material of the food industry for the production of refined oil and its sub products and the meal is mainly used to increase the protein content in animal food. The quality of soybean meal is evaluated by its protein content. Meal resulting from soybean produced in Brazil, specifically in southern region of Brazil, has been presenting a protein content lower than the demands of the market, what results in the product?s devaluation. The concentration of oil and protein in soybean seed is an inherited qualitative trait, but it is greatly influenced by the environment .Under the condition of cultivation in vitro it is difficult to control precisely the supply of carbon and nitrogen destined for the seeds. With the purpose of evaluating soybean?s genotype on the concentration of protein and oil of seeds developed in vivo and in vitro, immature seeds of cultivars CD 202 and CD 206 were removed from the mother-plant in the stage R5 and were cultivated in vitro, in a liquid milieu of cultivation which contained 20, 40 and 60 mM of glutamine. The seeds were incubated in glass flasks and agitated constantly during eight days at 25± 0,2 º C with 204,5 mM sucrose concentration. After that period of incubation it was determined the gain of fresh mass of the seeds. Afterwards it was also determined the oil and protein contents of the seeds developed in vitro and those developed in vivo. The fresh mass gain did not present a significant difference ( P>0,05) in the same cultivar when the glutamine concentration was altered , but between the two cultivars, fresh mass gain showed a significant difference (P<0,05) in the concentration of 40 and 60 mM of glutamine in the cultivar CD 206 as compared to the cultivar CD 202. The protein percentage in the 20 mM glutamine concentration did not present a significant difference (P>0,05) in the cultivar CD 206 cultivated in vivo. Nevertheless the opposite occurs in 40 and 60 mM of glutamine concentrations. Cultivar 202 when cultivated in vitro in 20 mM concentration showed a lower protein percentage than that of the seed cultivated in vivo. However, over 20 mM glutamine concentration, the cultivar CD 202 showed a high percentage of protein, what represents a good response to the increase of glutamine concentration. With an adequate supply of nitrogen for the seeds cultivated in vitro, the genotype did not limit the protein gain. Between the studied cultivars, protein percentage was always higher in the cultivar CD 206. Oil and protein concentrations were inversely related. The genotype has influence on soybean seeds composition, since it was observed that statistical differences in the oil and protein percentage remained unchanged even when a variation in the nitrogen supply to the seeds developed in vitro ocurred.
Santos, Maria Aparecida dos. "Identificação de QTL associados à simbiose entre bradyrhizobium japonicum/B. elkanii e a soja [Glycine max(L.) Merr.]." Universidade Estadual de Londrina. Centro de Ciências Biológicas. Programa de Pós-Graduação em Microbiologia, 2005. http://www.bibliotecadigital.uel.br/document/?code=vtls000108570.
Повний текст джерелаNitrogen (N) demand of soybean [Glycine max (L.) Merrill] can be supplied via biological nitrogen fixation (BNF) through the inoculation with selected Bradyrhizobium japonicum/B. elkanii strains, such that today no N-fertilizer is recommended for the crop in Brazil. However, traits related to BNF have not been lately evaluated in soybean breeding programs, with priority given to yield and resistance to diseases. The objective of this study was the identification of QTL (Quantitative Trait Loci) related to BNF using microsatellites (SSR) markers, in an F2:7 population of 157 Recombinant Imbred Lines (RILs), derived from the cross between parental cultivars with contrasting capacities of BNF, Bossier (high) and Embrapa 20 (medium). Soybean lines were grown under greenhouse conditions for the evaluation of the parameters of plant growth (shoot dry weight, SDW), and nodulation (nodule number, NN; nodule dry weight, NDW and the relation nodule dry weight/nodule number, NDW/NN). All parameters evaluated showed statistical differences (P= 0.05), indicating genetic variability among soybean lines. Sixteen markers located in six out of the twenty soybean linkage groups have been mapped, covering about 5% of the genome (151.6 cM). The regression analysis identified twelve significant associations in four linkage groups (B1, C2, D1b and H): three for shoot weight, four for nodule number, four for nodule weight and three for the medium value of nodule weight. All QTL had minor effects (R2 = 2,5 to 8,0%) similar to previous reports in an F2:3 of BRS 133 (low) x Embrapa 20 (medium) (Nicolás et al., 2005). However, seven QTL were confirmed in both populations, indicating that they might be effective in increasing BNF in soybean breeding programs.
Seibel, Neusa Fátima. "Caracterização, fracionamento e hidrólise enzimática dos componentes do resíduo do processamento da soja [Glycine Max (L.) Merrill], fibras dos cotilédones." Universidade Estadual de Londrina. Centro de Ciências Agrárias. Programa de Pós-Graduação em Ciência de Alimentos, 2006. http://www.bibliotecadigital.uel.br/document/?code=vtls000118533.
Повний текст джерелаSoybean derived ingredients: cotyledon fibers, defatted flour and protein concentrate were evaluated for chemical characteristics and functional properties. Potassium was the macro and iron the micro mineral in highest concentration in all the ingredients. The highest concentration of phytic acid was in the protein concentrate, as well as the highest concentration of isoflavones, however, the fibers had more genistin and daidzein and the total isoflavones in the fibers corresponded to 45% of the amount in the defatted flour. Dietary fibers had the best hydration properties and in properties related to oil emulsification had similar indexes to soybean flour, despite the lower protein concentration. The cell wall soybean cotyledon fiber was fractioned and the major component was hemicellulose, corresponding to 55% on the average. Based on monosaccharide composition of the cell wall fractions there were two groups: (1) cellulose and (2) pectin and hemicelluloses. Protein classification due to solubility in different solvents showed that defatted flour had a major protein fraction extracted with salt solution while the protein concentrate and dietary fibers had higher protein solubility with alkaline solution. The electrophoresis presented the major soybean proteins, ß-conglicinin and glicinin in cotyledon fibers and in protein ingredients. The protein extracted from fibers also reveled bands with molecular weigh next 30KDa, probably cell wall hydroxyproline-rich glycoproteins. After identifying the best conditions, the soybean fibers were hydrolyzed with carbohydrase and protease. The solid fraction of carbohydrase hydrolyzed fiber had higher protein concentration and increased hydrophilic properties, while the soluble fraction had 73% carbohydrates and 50% uronic acids of the initial quantity in samples. The protease hydrolyzed 54% of the total protein in the fiber samples, producing a solid fraction with 76% total dietary fiber. The soluble fraction had peptides of low molecular weigh, lower than 10KDa, and one band of molecular weigh close to 25KDa. The SEM micrographies confirmed the reduced particle in the milled sample in relation to the original sample. The physical structure of the original fiber hydrolyzed with protease had more superficial porosity than the reduced particle fiber. The hydrolyzed samples with carbohydrase presented a more compact structure.
Aoyagi, Luciano Nobuhiro. "Caracterização estrutural e transcricional dos fatores de transcrição R2R3-MYB no genoma da soja (Glycine max) em resposta a doenças." Universidade Estadual de Londrina. Centro de Ciências Exatas. Programa de Pós-Graduação em Biotecnologia, 2013. http://www.bibliotecadigital.uel.br/document/?code=vtls000188152.
Повний текст джерелаSoybean is one of the most important crops for the economy, since it is the main product of the agricultural sector and export of Brazil. Asian soybean rust caused by Phakopsora pachyrhizi, is a limiting factor for the crop expansion and a threat because of its potential yield losses and increase in cultivation costs. The control carried out with chemicals is expensive and cause undesirable impacts to humans and the environment, creating the need to seek for alternatives and equally effective control. A more profound understanding of the mechanisms of defense and resistance of soybean plants, as well as the search for genes associated with these processes represent a path to find ways to overcome the problems caused by FAS and other pathogens. Studies of the interaction of soybeans with FAS indicate that transcription factors (TF) MYB family are differentially expressed in resistant plants. In Arabidopsis, MYBs are extensively studied and have well-defined functions, including control over the defense of the plant. Despite evidence suggest a possible role of these TFs in controlling important processes in soybean, including defense response, there is a lack of knowledge, compared with Arabidopsis. MYB is currently the largest family of FT of G. max, and also in plants, indicating great potential to contain candidates that act to control important processes. Aiming to increase knowledge about these mechanisms, in this work we evaluated the family of MYB transcription factors. GmMYBs class R2R3 were identified from the soybean genome using bioinformatics (SMART, Pfam and MEME), and putative function was determined on the basis of phylogenetic tree construction and classification of subfamilies in GmMYBs using guides (AtMYB) with functions known. All MYB subfamilies of Arabidopsis thaliana and some soybean seen in previous works were formed, as well as new subgroups. The expression and transcriptional profile of GmMYBs R2R3 assays with FAS through analysis in banks of transcritomas (Soybase, LGE-Genosoja, Genevestigator) was performed and associated with determining the putative functions for search potential genes involved in the control mechanism defense. After the selection of target genes, induction by infection with FAS was assessed by quantitative PCR in real time at different times of inoculum (12, 24, 48, 72 and 96 hai) into two treatments (with or without inoculum of spores of P. pachyrhizi). Assays were performed in three genotypes, two resistant PI230970 (resistant) having Rpp2 allele and the allele that has Shiranui Rpp5, and showing lesions of the type RB in response to FAS and Williams 82 (susceptible) having TAN type lesions. Among the genes evaluated, there was especially those related to lignin synthesis with three GmMYBs induced in plant resistance in higher levels and faster, one GmMYB with unknown function and other possibly connected to modulation of responsive genes chitin, both induced by a longer period in the plant resistant.
Godoy, Leandro Pereira de. "Panorama genômico da estirpe semia 5079 (=CPAC 15) de Bradyrhizobium Japonicum, recomendada comercialmente para a cultura da soja (Glycine max (L) merr.)." Universidade Estadual de Londrina. Centro de Ciências Biológicas. Programa de Pós-Graduação em Microbiologia, 2007. http://www.bibliotecadigital.uel.br/document/?code=vtls000125585.
Повний текст джерелаThe biological nitrogen fixation (BNF) process with bacteria belonging to the species Bradyrhizobium japonicum and B. elkanii is crucial for the economical viability of the soybean crop in Brazil. A strain selection program began with the crop expansion in the earlier 1960s, aiming at the identification of bacteria adapted to the local environmental conditions and to the Brazilian cultivars; strain SEMIA 5079 (= CPAC 15) of B. japonicum was then recognized by its efficiency, competitiveness and adaptability to stressful environmental conditions, therefore it has been employed in commercial inoculants since 1992. Few rhizobial genomes were completly sequenced in the last decade, and allowed an impressive increase in the genetic knowledge of these bacteria. In this context, this study aimed at the partial sequencing of the genome of strain SEMIA 5079, searching for genes related to the saprophytic capacity, competitiveness and efficiency of the BNF process. The readings of shotgun libraries built with SEMIA 5079 total DNA resulted in a real covering of 13.17% of the genome, with a total of 1.371 CDSs (coding sequences) annotated, 729 with known functions, 312 classified as conserved hypothetical and 330 as hypothetical. The functional classification of the CDSs in the database COG (Clusters of Orthologous Groups of proteins) identified putative genes in almost all classes. Furthermore, the comparison with strain USDA 110 of B. japonicum, sequenced in 2002 by Japanese researchers, has also demonstrated similar percentages of CDSs classified in the categories of COG, an important indication to support the strategy of partial genome sequencing of a bacterium. In relation to the KEGG (Kyoto Encyclopedia of Genes and Genomes) database, the CDSs of SEMIA 5079 have shown similarity with the sequences of 71 other organisms, but in a higher number with B. japonicum strain USDA 110, and with Bradyrhizobium sp. strains BTAi1 and ORS278. Fourteen transposases were found, most related to insertion sequences, indicating high plasticity of the genome of SEMIA 5079; furthermore, 4.54% of the CDSs were classified as paralog genes, also favoring environmental adaptation. Several CDSs were annotated as genes that codify proteins related to the nodulation, as nodB, nodD2 and nodW genes, and to the BNF, as nifE, fixP, fixQ, and fixR genes, in addition to other proteins involved in the cycle of N. The partial sequencing of the genome of strain SEMIA 5079 has also identified 5.76% of CDSs participating in almost all pathways of xenobiotic biodegradation, and might represent a reservoir of important genes with biotechnological potential.
Книги з теми "Glycan code"
Potsdam, Germany) International Beilstein Symposium on Glyco-Bioinformatics (2nd 2011. Proceedings of the 2nd Beilstein Symposium on Glyco-Bioinformatics: Cracking the sugar code by navigating the glycospace : June 27th-July 1st, 2011, Potsdam, Germany. Berlin: Logos Verlag Berlin GmbH, 2012.
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Šebestík, Jaroslav, Milan Reiniš, and Jan Ježek. "Sugar Code (Glycocode)." In Biomedical Applications of Peptide-, Glyco- and Glycopeptide Dendrimers, and Analogous Dendrimeric Structures, 23–27. Vienna: Springer Vienna, 2012. http://dx.doi.org/10.1007/978-3-7091-1206-9_3.
Повний текст джерелаLechner, J. T., J. Stierstorfer, and T. M. Klapötke. "Crystal Structure and Characterization of the Well-Known Plasticizers Ethylene Glycol Dinitrate, Diethylene Glycol Dinitrate and Triethylene Glycol Dinitrate." In Future Developments in Explosives and Energetics, 57–12. Royal Society of Chemistry, 2023. http://dx.doi.org/10.1039/9781839162350-00057.
Повний текст джерелаLechner, J. T., J. Stierstorfer, and T. M. Klapötke. "Crystal Structure and Characterization of the Well-Known Plasticizers Ethylene Glycol Dinitrate, Diethylene Glycol Dinitrate and Triethylene Glycol Dinitrate." In Future Developments in Explosives and Energetics, 57–12. Royal Society of Chemistry, 2023. http://dx.doi.org/10.1039/9781788017855-00057.
Повний текст джерелаCoppens, Philip. "Electron Density Studies of Molecular Crystals." In X-Ray Charge Densities and Chemical Bonding. Oxford University Press, 1997. http://dx.doi.org/10.1093/oso/9780195098235.003.0014.
Повний текст джерелаKelly, Alan. "Consistency and Change." In Molecules, Microbes, and Meals. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780190687694.003.0006.
Повний текст джерелаBecker, Richard C., and Frederick A. Spencer. "Novel Anticoagulants." In Fibrinolytic and Antithrombotic Therapy. Oxford University Press, 2006. http://dx.doi.org/10.1093/oso/9780195155648.003.0023.
Повний текст джерелаТези доповідей конференцій з теми "Glycan code"
Schuberth, Ralf, Ingo Prahl, Harald Weiss, and Carlo Unverzagt. "SYNTHESIS OF THE LEC-10 NONASACCHARIDE, A BISECTED AND CORE-FUCOSYLATED N-GLYCAN." In XXIst International Carbohydrate Symposium 2002. TheScientificWorld Ltd, 2002. http://dx.doi.org/10.1100/tsw.2002.407.
Повний текст джерелаMehta, Anand S., Mary Ann Comunale, Siddhartha Rawat, Jessica Garner, Lucy Betesh, Mengjun Wang, Laura Steel, and Michael Bouchard. "Abstract 4982: Increased core fucosylation, a glycan alteration associated with cancer, is the result of hepatocyte dedifferentiation." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-4982.
Повний текст джерелаJokar, Amir, Steven J. Eckels, and Mohammad H. Hosni. "Evaluation of Heat Transfer and Pressure Drop for the Heater-Core in an Automotive Heat Pump System." In ASME 2004 International Mechanical Engineering Congress and Exposition. ASMEDC, 2004. http://dx.doi.org/10.1115/imece2004-60824.
Повний текст джерелаWoo, Kyoungja, and Byeong-Seon Kim. "Synthesis and bioconjugation of CdSe/CdS core/shell nanoparticles to poly(ethylene glycol) analogues." In Microelectronics, MEMS, and Nanotechnology, edited by Dan V. Nicolau. SPIE, 2005. http://dx.doi.org/10.1117/12.637955.
Повний текст джерелаSano, T., and H. Schmidt. "Dual wavelength optofluidic distributed feedback dye laser on a single PDMS chip." In CLEO: Applications and Technology. Washington, D.C.: Optica Publishing Group, 2023. http://dx.doi.org/10.1364/cleo_at.2023.jw2a.1.
Повний текст джерелаSaini, Simarjeet S., Chris Stanford, Juhee Park, Phillip DeShong, William E. Bentley, and Mario Dagenais. "APTES and APES Detection and Glyco Sensing Using Etched Core Fiber Bragg Grating Sensors." In Optical Fiber Sensors. Washington, D.C.: OSA, 2006. http://dx.doi.org/10.1364/ofs.2006.thf4.
Повний текст джерелаEbong, E. E., D. C. Spray, and J. M. Tarbell. "The Roles of HS and Its Glypican-1 Core Protein in Flow-Induced Endothelial NOS Activation and Cell Remodeling." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53294.
Повний текст джерелаWoo, Hee-Suk, Yun-Seok Hong, Young-Soon Lim, Jung-Yean Park, Hee-Jae Ahn, and Choong-Dong Lee. "Structural Analysis for Ethylene Oxide Reactor." In ASME 2007 Pressure Vessels and Piping Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/pvp2007-26486.
Повний текст джерелаFork, Richard L., H. L. Fragnito, P. C. Becker, and C. H. Hirlimann. "Multipass amplifier for femtosecond optical pulses." In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1988. http://dx.doi.org/10.1364/oam.1988.mn1.
Повний текст джерелаKornhauser, Alan A. "Aqua-Ammonia as an Environmentally Acceptable Low Temperature Brine." In ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-62684.
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George and Hawley. PR-015-10602-R01 Effects of Liquid Contamination on Ultrasonic Flow Meter Performance. Chantilly, Virginia: Pipeline Research Council International, Inc. (PRCI), August 2012. http://dx.doi.org/10.55274/r0010787.
Повний текст джерелаAltstein, Miriam, and Ronald J. Nachman. Rational Design of Insect Control Agent Prototypes Based on Pyrokinin/PBAN Neuropeptide Antagonists. United States Department of Agriculture, August 2013. http://dx.doi.org/10.32747/2013.7593398.bard.
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