Дисертації з теми "Genetics and Genome Biology"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-50 дисертацій для дослідження на тему "Genetics and Genome Biology".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте дисертації для різних дисциплін та оформлюйте правильно вашу бібліографію.
Chew, Wei Leong. "Postnatal Genome Editing With CRISPR." Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:33493352.
Повний текст джерелаMedical Sciences
Omilian, Angela Ruggieri. "Features of Daphnia genome evolution." [Bloomington, Ind.] : Indiana University, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3243783.
Повний текст джерелаTitle from PDF t.p. (viewed Nov. 18, 2008). Source: Dissertation Abstracts International, Volume: 67-12, Section: B, page: 6862. Adviser: Michael Lynch.
Chung, Hattie. "Genome evolution in structured systems." Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:33493565.
Повний текст джерелаSystems Biology
Chen, Stacy Yen-chun. "Genome-wide analysis of yeast meiotic recombination landscape." Diss., Search in ProQuest Dissertations & Theses. UC Only, 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3390037.
Повний текст джерелаSimonson, Matthew A. "Polygenic analysis of genome-wide SNP data." Thesis, University of Colorado at Boulder, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=3562047.
Повний текст джерелаOne of the central motivators behind genetic research is to understand how genetic variation relates to human health and disease. Recently, there has been a large-scale effort to find common genetic variants associated with many forms of disease and disorder using single nucleotide polymorphisms (SNPs). Several genome-wide association (GWAS) studies have successfully identified SNPs associated with phenotypes. However, the effect sizes attributed to individual variants is generally small, explaining only a very small amount of the genetic risk and heritability expected based on the estimates of family and twin studies. Several explanations exist for the inability of GWAS to find the "missing heritability."
The results of recent research appear to confirm the prediction made by population genetics theory that most complex phenotypes are highly polygenic, occasionally influenced by a few alleles of relatively large effect, and usually by several of small effect. Studies have also confirmed that common variants are only part of what contributes to the total genetic variance for most traits, indicating rare-variants may play a significant role.
This research addresses some of the most glaring weaknesses of the traditional GWAS approach through the application of methods of polygenic analysis. We apply several methods, including those that investigate the net effects of large sets of SNPs, more sophisticated approaches informed by biology rather than the purely statistical approach of GWAS, as well as methods that infer the effects of recessive rare variants.
Our results indicate that traditional GWAS is well complemented and improved upon by methods of polygenic analysis. We demonstrate that polygenic approaches can be used to significantly predict individual risk for disease, provide an unbiased estimate of a substantial proportion of the heritability for multiple phenotypes, identify sets of genes grouped into biological pathways that are enriched for associations, and finally, detect the significant influence of recessive rare variants.
Wesolowska, Natalia. "Modification and nuclear organization of the Drosophila melanogaster genome." Thesis, The Johns Hopkins University, 2013. http://pqdtopen.proquest.com/#viewpdf?dispub=3575013.
Повний текст джерелаThe success of Drosophila as a system for genetic analysis is closely linked to its amenability to genetic manipulation. Part 1 of the dissertation elucidates a novel scheme for long-range targeted manipulation of genes. We integrated an 80-kb genomic fragment at its endogenous locus, utilizing a targeted attP attachment site for the phiC31 integrase. We achieved single-copy reduction of the resulting region duplication by inducing recombinational DNA repair. We showed that this two-step scheme of integration and reduction is efficient and useful for delivering modifications. We established a vector configuration that facilitates the recovery of modifications. The integrating genomic fragment allowed for delivery of a new attachment site at 70 kb from the existing attP into a new locus, making it susceptible to targeted mutagenesis. We extrapolate that with this scheme, only 1 200 lines bearing att-sites throughout the genome would suffice to render all Drosophila genes amenable to targeted mutagenesis. Excitingly, this method should be readily applicable to other systems.
In Part 2 of the dissertation, I explored the question of telomere organization in Drosophila. Telomeres demarcate the ends of linear chromosomes to distinguish them from broken ends. In yeast, they cluster at the periphery of the nucleus establishing a compartment of silent chromatin. To bring insight into telomere organization in a higher organism, we followed EGFPlabeled Drosophila telomeric protein HOAP in vivo and found that the 16 telomeres cluster into 4-6 foci per nucleus in somatic tissues. Interestingly, HOAP signal intensity in the clusters doubles in interphase, potentially due to loading of HOAP to newly replicated telomeres. We tested several predictions about rules governing clustering. First, by inspecting mutant embryos that develop as haploids, we found that clustering is not mediated by associations between homologs. Second, by demonstrating clustering capability for a telomere of novel sequence, we eliminated DNA sequence homology and identity as important factors. Third, by marking both ends of a chromosome, we ruled out predominance of intra-chromosomal interactions. We propose that clustering is indiscriminate of sequence and is likely maintained by a yet undetermined factor.
Badhwar, AmanPreet. "Identification and characterization of rearrangements in the vervet monkey genome." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=101702.
Повний текст джерелаTo study the evolutionary dynamics of centromere formation, I identified and validated the alpha-satellite repeat as a centromere-specific marker in the vervet using comparative genomics, sequence analysis and hybridization screening. I developed criteria to infer the position of vervet bacterial artificial chromosome (BAC) inserts based on alpha-satellite monomer content. In a complementary approach, I demarcated the pericentromeric boundaries in human and identified vervet BAC clones that mapped orthologously to these regions.
In addition to centromeric analyses, I developed methodologies to detect other genome rearrangements, in particular vervet deletion/human insertion and vervet translocation events. The tools and approaches developed in this thesis will prove useful in cataloguing additional vervet genome rearrangements.
Young, Adrian. "The Evolutionary Feedback between Genetic Conflict and Genome Architecture." Thesis, Harvard University, 2014. http://dissertations.umi.com/gsas.harvard:11482.
Повний текст джерелаLi, Hubo. "Genome-Wide RNAi Screens for Novel Regulators of Acute Myeloid Leukemia." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:14226105.
Повний текст джерелаCanver, Matthew. "Elucidation of Mechanisms of Fetal Hemoglobin Regulation by CRISPR/Cas9 Mediated Genome Editing." Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:33493407.
Повний текст джерелаMedical Sciences
Fernandes, Caroline. "Genome-wide screen for novel regulators of Parkinson's disease genes in «Drosophila melanogaster»." Thesis, McGill University, 2011. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=103725.
Повний текст джерелаIntroduction : L'altération de la fonction mitochondriale entraîne la dégénérescence de certains neurones chez les personnes atteintes de maladies neurodégénératives dont la maladie de Parkinson. Les cellules saines sont dotées d'un système de contrôle leur permettant de faire face et de réparer les dysfonctionnements des mitochondries et ainsi de préserver leur intégrité. Des études récentes ont révélé l'implication de deux gènes à l'origine de syndromes parkinsoniens autosomiques récessifs, Pink1 et Parkin, dans une voie de signalisation commune contrôlant le maintien de la fonction mitochondriale. Pink1 et Parkin interviennent ensemble dans l'isolation et la dégradation des mitochondries défectueuses. Cependant, à l'heure actuelle, les mécanismes moléculaires contrôlant ce processus restent à élucider. Méthodes : Le but de notre étude a été d'identifier de nouveaux régulateurs de la voie de signalisation Pink1/Parkin par crible génétique dans un modèle drosophile de la maladie de Parkinson. Pour cela, nous avons criblé une collection de lignées de drosophiles déficientes sur les chromosomes deux et trois pour leur capacité à modifier (augmenter ou diminuer) le phénotype de posture de l'aile caractéristique de la mutation de Pink1/Parkin. Résultats: Nous avons identifié plusieurs régions cytologiques qui interagissent fortement avec Parkin et/ou Pink1. Quatre de ces régions ont été disséquées de façon à révéler cinq gènes. Parmi eux, opa1 et drp1 avaient déjà été impliques dans la voie de signalisation Pink1/Parkin. Les trois autres gènes p60, β4galNacTA et debra représentent de nouveaux régulateurs de la fonction de Pink1 et Parkin. Conclusion/implications : D'une part, l'identification non biaisée de gènes déjà connus comme interagissant avec Pink1/Parkin démontre la validité de cette approche. D'autre part, la découverte de nouveaux gènes candidats de la voie Pink1/Parkin pour le maintien de l'intégrité mitochondriale permettra de mieux comprendre les mécanismes moléculaires contrôlant ce processus et aidera à l'élaboration de traitements.
Rogers, Jameson Kerr. "Biosensing for Multiplexed Genome Engineering: Applications in Renewable Chemical Production." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:17467367.
Повний текст джерелаEngineering and Applied Sciences - Engineering Sciences
Shanmugasundram, Achchuthan. "Genome annotation and metabolic reconstruction of apicomplexan parasites." Thesis, University of Liverpool, 2014. http://livrepository.liverpool.ac.uk/2004940/.
Повний текст джерелаSchiavi, Alicia. "Direct assessment and validation of allele specific transcription factor binding in the human genome." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=114371.
Повний текст джерелаLa caractérisation de la variation génétique a mis l'accent sur les loci d'expression de caractères quantitatifs(eLCQ); cependant, l'évaluation directe des variations régulatrices en cis nécessite des approches allèle-spécifique (cis-rSNPs). La mesure de l'expression allélique (EA) est très efficace puisque les perturbations environnementales et les influences en trans sont réduites. Les résultats indiquent que les différences d'EA peuvent affecter jusqu'à 30% des loci chez un individu. Les polymorphismes responsable de telles variations peuvent être identifiés par cartographie des différences d'EA en utilisant des puces de génotypage Illumina. Ainsi, plus de 50% de la variance en EA de la population est expliqué par la cartographie des cis-rSNPs. Des études ont montré que ces cis-rSNPs ont été impliqués dans des différences de liason de facteurs de transcription (FT). Celà suggère que l'étude du mode d'action de ces FT pourrait être approfondie par l'utilisation comme outil des polymorphismes dans la population. Nous avons appliqué cette approche au FT NF-κB et analysé les conséquences de l'inactivation de ce gène à l'échelle du genome. Des données récentes montrent l'implication de NF-κB dans la réponse immunitaire et son motif de liaison à l'ADN est retrouvé enrichi dans les cellules lymphoblastoïdes humaines (LCL), surtout au niveau des promoteurs et des activateurs transcriptionnels. Nous avons croisé les cis-rSNPs cartographiés dans des LCLs des populations HapMap YRI et CEU, ainsi que des SNPs de contrôles, avec des données d'immunoprécipitation de chromatine suivi de séquençage à haut débit (ChIP-seq) utilisant l'anticorps NF-κB du projet ENCODE et accessible au public. Des analyses préliminaires des régions contenant les cis-rSNPs candidats ont montré un enrichissement des sites de liaison pour le facteur NF-κB par rapport aux sites contrôles. En effet, 39% des sites candidats sont situés dans un site de liaison pour NF-kB. Afin d'étudier le rôle potentiel des cis-rSNPs sur l'EA différentielle, nous avons réalisé des expériences d'induction de TNF-α couplé à l'inhibition de NF-κB dans les LCLs suivie par l'analyses de l'EA sur des puces de génotypage Illumina 5M. Nous avons ensuite comparé ces données avec la cartographie de cis-rSNPs dans des LCLs générée dans notre laboratoire.Nos données sont composées de cis-rSNPs associés à l'EA différentielle de loci suite à la perturbation de NF-κB et classés par valeur p (pv; top10= les 10 valeurs les plus significatives) et ont été croisées avec des données accessibles au public. Ces données sont composées des coordonnées de pics de ChIP-seq et des sites de liaison TRANSFAC pour le facteur NF-κB et de ses co-régulateurs transcriptionnels connus. Les loci montrant des différences d'EA suite à la perturbation de NF-κB et avec 1 ou plusieurs des cis-rSNPs (« top3 » pv) hétérozygotes dans les individus étudiés étaient aux nombre de 581. La recherche de ces cis-rSNPs « top 3 » dans les sites de liaison (pics de ChIP-seq) dans les LCLs ou dans un site de TRANSFAC pour NF-κB ou pour un de ses co-régulateurs transcriptionnels montrent un enrichissement significatif (64% des loci) avec un ratio supérieur à 5 par rapport aux SNPs de contrôles.L'analyse bioinformatique suggère que les SNPs identifiés sont essentiels pour la liason de NF-κB. Une étude complémentaire à consisté à perturber le FT SNAI1 probablement associé au gène WNT4 et présentant un rôle important dans les fibroblastes (FB). Cependant, nous n'avons pas été en mesure de reproduire l'effet de SNAI1 sur WNT4 in vivo.Nous avons ensuite comparé les rôles régulateurs de NF-κB et de SNAI1 dans les LCLs et les FBs respectivement (par inactivation de ces gènes). Nous avons observé que NF-κB a un effet régulateurs sur environ 33% des loci dans les LCLs contre seulement 2 % pour SNAI1 dans des FBs.Cette étude supporte l'utilisation de perturbations de l'expression de FT pour étudier le rôle clé de FTs régulateurs dans un type cellulaire donné.
Yang, Joyce Lichi. "Developments in Human Pluripotent Stem Cell Genome Engineering and in Situ Sequencing Technologies." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:17467524.
Повний текст джерелаMedical Sciences
Oakes, Christopher Charles. "DNA methylation in male germ cells : the acquisition and maintenance of unique genome-wide patterns." Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=103175.
Повний текст джерелаBoettger, Linda M. "Complex Forms of Structural Variation in the Human Genome: Haplotypes, Evolution, and Relationship to Disease." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:14226090.
Повний текст джерелаRussell, Shelbi Lianne. "Mode and Fidelity of Bacterial Symbiont Transmission and Its Impact on Symbiont Genome Evolution." Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:33493576.
Повний текст джерелаBiology, Organismic and Evolutionary
Herdy, Joseph R. III. "SMALL RNA EXPRESSION DURING PROGRAMMED REARRAGEMENT OF A VERTEBRATE GENOME." UKnowledge, 2014. http://uknowledge.uky.edu/biology_etds/25.
Повний текст джерелаFredman, David. "Computational exploration of human genome variation /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7140-025-7/.
Повний текст джерелаRios, Villanueva Xavier. "Toward Multiplex Genome Engineering in Mammalian Cells." Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:11179.
Повний текст джерелаPettersson, Mats. "Modeling Genome Evolution : Creation, Change and Destruction." Doctoral thesis, Uppsala University, Department of Evolution, Genomics and Systematics, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8163.
Повний текст джерелаHistorically, evolution has been studied either by looking at morphological traits in living organisms and the fossil record, or by using bioinformatics and comparative genomics. While highly useful for deducing evolutionary history, these approaches are not particularly well suited for studying the mechanisms of evolution. In order to address such issues, other methods are needed. Mathematical modelling is one of the most powerful options available, and it is the approach used in this thesis. By constructing models of biological systems, the work aims to resolve some of the many unresolved questions regarding evolutionary processes, such as how new genes evolve and how selection acts in fragmented populations. Some answers have been reached, and thus the thesis makes a small contribution to our overall understanding of evolution.
The creation of novel genes was studied both directly and by extension of an analogous system, which revolved around reversion of a frameshift mutant. The results pointed to gene amplification as a likely mechanism for both reversion of the frameshift mutant and creation of new genes.
Selection in fragmented populations is shown to be effective even when sub-populations, rather than individuals, are competing against each other. Modeling of a system of bacterial symbionts living in aphids indicates that, although the bacterial population within a single host is small and subject to rampant genetic drift, the bacterial population as a whole is regulated by selection on the host level. Thus, deleterious mutations do no accumulate and the population maintains its fitness over time.
Forgetta, Vincenzo. "Systematic search for Salmonella-susceptibility quantitative trait loci in the chicken using a whole genome scan approach." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=33758.
Повний текст джерелаVarsally, Wazeer Mohammad. "Inferring biological networks from genome-wide transcriptional and fitness data." Thesis, University of Birmingham, 2014. http://etheses.bham.ac.uk//id/eprint/5120/.
Повний текст джерелаSöderberg, Jonas. "Surviving the ratchet : Modelling deleterious mutations in asexual populations." Doctoral thesis, Uppsala universitet, Molekylär evolution, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-157897.
Повний текст джерелаGourlay, Elaine Margaret. "Strategies for human genome modification using engineered nucleases and transcription factors." Thesis, University of Glasgow, 2015. http://theses.gla.ac.uk/5875/.
Повний текст джерелаCong, Le. "Genome Engineering Technology and Its Application in Mammalian Cells." Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:11309.
Повний текст джерелаSeshadri, Chitra. "Genome wide epigenetic analyses of Araptus attenuatus, a bark beetle." VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4167.
Повний текст джерелаSyed, Salahuddin. "Nonreplicative DNA Helicases Involved in Maintaining Genome Stability." Scholar Commons, 2016. http://scholarcommons.usf.edu/etd/6408.
Повний текст джерелаRobinson, Jason M. "Functional Significance of mtDNA Cytosine Modification Tested by Genome Editing." VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4561.
Повний текст джерелаSoleimani, Vahab D. "Genome dynamics in barley (Hordeum vulgare L) cultivars: Molecular diversity, evolution, and DNA fingerprinting." Thesis, University of Ottawa (Canada), 2005. http://hdl.handle.net/10393/29264.
Повний текст джерелаRamdath, Ramona Sherry. "Aneuploidy: Using genetic instability to preserve a haploid genome?" University of Toledo Health Science Campus / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=mco1243954510.
Повний текст джерелаAltheide, Tasha Kay. "Comparative population genetics of the Hominoidea: An investigation of locus-specific and genome-wide influences." Diss., The University of Arizona, 2002. http://hdl.handle.net/10150/279941.
Повний текст джерелаGrandhi, Sneha. "HIGH-THROUGHPUT ANALYSIS OF THE HUMAN MITOCHONDRIAL GENOME REVEALS ITS DYNAMICS, FUNCTION, AND SIGNALS OF SELECTION IN CANCER." Case Western Reserve University School of Graduate Studies / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1525450447304917.
Повний текст джерелаMosberg, Joshua Adam Weintrob. "Studying and Improving Lambda Red Recombination for Genome Engineering in Escherichia coli." Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:10777.
Повний текст джерелаCook, Kristen. "Regulation of Genome-Wide Transcriptional Stress Responses in Saccharomyces cerevisiae." Thesis, Harvard University, 2011. http://dissertations.umi.com/gsas.harvard:10032.
Повний текст джерелаWang, Xinchen Ph D. Massachusetts Institute of Technology. "Deciphering genetic associations using genome-wide epigenomics approaches." Thesis, Massachusetts Institute of Technology, 2017. http://hdl.handle.net/1721.1/111239.
Повний текст джерелаThis electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Cataloged from student-submitted PDF version of thesis.
Includes bibliographical references.
Genetic mapping of the drivers of complex human phenotypes and disease through the genome-wide association study (GWAS) has identified thousands of causal genetic loci in the human population. However, genetic mapping approaches can often only reveal a particular causal locus, not the molecular mechanism through which it acts. Biological interpretation of these genetic results is thus a bottleneck for turning results from GWAS into meaningful biological insights for human biology. Genetic mapping of complex human traits has revealed that most common variants influencing human phenotypes have weak effect sizes and reside outside protein-coding regions, complicating biological interpretation of their function. In this thesis we use computational and experimental approaches to study the non-coding genome. In particular, we focus on using epigenomic signatures to characterize non-coding transcriptional regulatory elements and predict regulatory segments of DNA disrupted by genetic variants. In Chapter 2, we describe how genome-wide maps of epigenomic modifications can be used to characterize and discover new GWAS loci. In Chapter 3, we outline an experimental method for the high-throughput assessment of putative transcriptional regulatory elements. In summary, our research highlights the value of interpreting human genetics information through an epigenomic lens, and provides a glimpse into the possible biological insights that manifest from the intersection of these two areas of research.
by Xinchen Wang.
Ph. D.
Liu, Yang. "Data mining methods for single nucleotide polymorphisms analysis in computational biology." HKBU Institutional Repository, 2011. http://repository.hkbu.edu.hk/etd_ra/1287.
Повний текст джерелаMignogna, Kristin. "Genome-Wide Systems Genetics of Alcohol Consumption and Dependence." VCU Scholars Compass, 2019. https://scholarscompass.vcu.edu/etd/5946.
Повний текст джерелаWidmayer, Heather. "Biogeographical patterns associated with genome-wide genetic differentiation in a widespread species of South American Blepharoneura fruit flies (Tephritidae)." Thesis, University of Iowa, 2018. https://ir.uiowa.edu/etd/6337.
Повний текст джерелаCrisci, Jessica L. "On Identifying Signatures of Positive Selection in Human Populations: A Dissertation." eScholarship@UMMS, 2013. https://escholarship.umassmed.edu/gsbs_diss/664.
Повний текст джерелаCrisci, Jessica L. "On Identifying Signatures of Positive Selection in Human Populations: A Dissertation." eScholarship@UMMS, 2006. http://escholarship.umassmed.edu/gsbs_diss/664.
Повний текст джерелаOjha, Sohita. "Identifying Genetic Factors Influencing Sperm Mobility Phenotype in Chicken using Genome Wide Association Studies, Primordial Germ Cell Transplantation, and RNAseq." Thesis, University of Arkansas, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10638065.
Повний текст джерелаSperm mobility is a major determinant of male fertility in chicken. In spite of low heritability of reproductive traits, sperm mobility has high heritability index which suggests presence of quantitative trait loci (QTLs) governing the trait. Our research focused on three objectives: i) to identify the QTLs affecting low mobility phenotype in chicken, ii) to understand the impact of Sertoli-cells and germ cells interactions in influencing the mobility phenotype and iii) to identify the genes and gene networks differentially expressed in male and female PGCs. To detect the QTLs, genome wide association studies (GWAS) was conducted which revealed the presence of multiple minor alleles influencing the trait and indicated the role of epistasis. The second section of research involved isolation, culture and transfer of primordial germ cells (PGCs) to create high line germ line chimera chicken carrying low line PGCs. We established the culture of chicken PGCs isolated from the embryonic blood in a feeder free culture conditions but could not detect the presence of low line genotype in the semen of transgenic males. Our final study involved RNA-sequencing (RNAseq) of male and female PGCs to identify differentially expressed genes from their transcriptomes. We identified five candidate genes: 3-hydroxy-3-methylglutaryl CoA reductase (HMGCA), germ cell-less (GCL), SWIM (zinc finger SWIM domain containing transcription factor), SLC1A1 (solute carrier family 1 member 1), UBE2R2L (ubiquitin conjugating enzyme) and validated their expression level in male and female PGCs by RT-qPCR. GCL was exclusively expressed in males while SLC1A1 & UBE2R2L were expressed only in female cPGCs. This present study provides novel gender specific germ cell markers in the broiler chicken. These results will help in elucidating the genetic programming of gender specific germ line development in broilers.
Kapoustina, Oxana. "The identification of genes regulated by Interferon Regulatory Factors (IRF) 1 and 8 in the context of pathogen challenge by ChIP on Chip and genome wide transcription profiling approaches." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=86565.
Повний текст джерелаLes facteurs régulateurs interférons (IRFs) sont une famille de régulateurs transcriptionnels essentiels à la différenciation et la croissance cellulaire, à l'oncogénèse et au bon maintien du système immunitaire. IRF1 et IRF8 jouent un role clé dans l'immunité de l'organisme par la régulation de la différenciation des lignées cellulaires myéloïdes, nottament le développement de cellules immunitaires comme les macrophages, les cellules tueuses naturelles (NK) et les cellules dendritiques. Ces deux facteurs de transcription sont indispensables pour la résistance aux infections virales et bactériennes. Nous avons optimisé une technique d'immunoprécipitation de la chromatine (ChIP) afin d'y utiliser des anticorps ciblant IRF1 et IRF8, et ce à partir d'une lignée cellulaire J774 suivant un stimulation par interféron gamma et par oligonucléotides CpG (IFNγ/CpG). En combinant cette technique ChIP à un microarray 244K comprenant exons et promoteurs (ChIP on Chip), nous avons identifiés respectivement 201 et 303 sites d'ancrages pour chacun de IRF1 et IRF8. Certaines cibles transcriptionnelles déjà connues pour IRF1 (OAS1b) et IRF8 (IFNb) ainsi que de nouveaux loci de control transcriptionel ont été identifiés. Parmi les catégories par fonction relevées par Ontologie de gène (GO), une des catégories majeures des deux listes de gènes générées fut pour les gènes reliés à la "réponse immunitaire''. La comparaison entre les gènes cibles de transcription pour IRF1 et IRF8 par "ChIP on Chip'' a révélé 19 gènes représentés dans les deux cas. La majeure partie de ces gènes sont reliés à la "réponse immunitaire'' par GO et ils incluent les cibles suivantes avec détection très significative: Gbp6, Mx2, Tnfsf13b, H2-T24, et Ifit1. En parallèle, des macrophages dérivés de la moëlle osseuse de souris (BMDMs) furent utilisés pour l'étude du profile transcriptionel par microarray d'une génération F2 générée avec des so
Martin, Kyle J. "Genomic characterization of cyclostome Dlx gene family members: Insight into the evolution of the chordate genome and body plan from the organizational and transcriptional regulatory properties of Dlx genes in the petromyzontiformes (lamprey) and the hyperotreti (Hagfish)." Thesis, University of Ottawa (Canada), 2009. http://hdl.handle.net/10393/28234.
Повний текст джерелаLyle, Suzanne McLean. "Error Correcting Codes and the Human Genome." Digital Commons @ East Tennessee State University, 2010. https://dc.etsu.edu/etd/1689.
Повний текст джерелаKim, Kieun Mohan Chilukuri K. "Parallel hierarchical adaptive genetic algorithm for genome sequencing." Related Electronic Resource: Current Research at SU : database of SU dissertations, recent titles available full text, 2003. http://wwwlib.umi.com/cr/syr/main.
Повний текст джерелаKohrn, Brendan F. "An Efficient Pipeline for Assaying Whole-Genome Plastid Variation for Population Genetics and Phylogeography." PDXScholar, 2017. https://pdxscholar.library.pdx.edu/open_access_etds/4007.
Повний текст джерелаLi, Xiang. "STRESS-INDUCED GENETIC CHANGE IN FLAX REVEALS GENOME VARIATION MECHANISM." Case Western Reserve University School of Graduate Studies / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=case1565964370435691.
Повний текст джерелаBurrows, Anna. "Genome-Wide Loss-of-Function Genetic Screens Identify Novel Senescence Genes and Putative Tumor Suppressors." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10191.
Повний текст джерела