Готові списки джерел за темами / GA200X

Добірка наукової літератури з теми "GA200X"

Автор: Grafiati
Опубліковано: 10 грудня 2022
Оновлено: 28 січня 2023

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями

Оберіть тип джерела:

Зміст

Ознайомтеся зі списками актуальних статей, книг, дисертацій, тез та інших наукових джерел на тему "GA200X".

Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.

Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.

Статті в журналах з теми "GA200X"

1

Zhang, Chenhao, Xin Nie, Weilong Kong, Xiaoxiao Deng, Tong Sun, Xuhui Liu, and Yangsheng Li. "Genome-Wide Identification and Evolution Analysis of the Gibberellin Oxidase Gene Family in Six Gramineae Crops." Genes 13, no. 5 (May 12, 2022): 863. http://dx.doi.org/10.3390/genes13050863.

Повний текст джерела
Анотація:
The plant hormones gibberellins (GAs) regulate plant growth and development and are closely related to the yield of cash crops. The GA oxidases (GAoxs), including the GA2ox, GA3ox, and GA20ox subfamilies, play pivotal roles in GAs’ biosynthesis and metabolism, but their classification and evolutionary pattern in Gramineae crops remain unclear. We thus conducted a comparative genomic study of GAox genes in six Gramineae representative crops, namely, Setaria italica (Si), Zea mays (Zm), Sorghum bicolor (Sb), Hordeum vulgare (Hv), Brachypodium distachyon (Bd), and Oryza sativa (Os). A total of 105 GAox genes were identified in these six crop genomes, belonging to the C19-GA2ox, C20-GA2ox, GA3ox, and GA20ox subfamilies. Based on orthogroup (OG) analysis, GAox genes were divided into nine OGs and the number of GAox genes in each of the OGs was similar among all tested crops, which indicated that GAox genes may have completed their family differentiations before the species differentiations of the tested species. The motif composition of GAox proteins showed that motifs 1, 2, 4, and 5, forming the 2OG-FeII_Oxy domain, were conserved in all identified GAox protein sequences, while motifs 11, 14, and 15 existed specifically in the GA20ox, C19-GA2ox, and C20-GA2ox protein sequences. Subsequently, the results of gene duplication events suggested that GAox genes mainly expanded in the form of WGD/SD and underwent purification selection and that maize had more GAox genes than other species due to its recent duplication events. The cis-acting elements analysis indicated that GAox genes may respond to growth and development, stress, hormones, and light signals. Moreover, the expression profiles of rice and maize showed that GAox genes were predominantly expressed in the panicles of the above two plants and the expression of several GAox genes was significantly induced by salt or cold stresses. In conclusion, our results provided further insight into GAox genes’ evolutionary differences among six representative Gramineae and highlighted GAox genes that may play a role in abiotic stress.
Стилі APA, Harvard, Vancouver, ISO та ін.
2

He, Liang, Lu, Wang, Liu, Ma, Zuo, Sun, Chen, and Mao. "Genome-Wide Identification and Expression Analysis of GA2ox, GA3ox, and GA20ox Are Related to Gibberellin Oxidase Genes in Grape (Vitis Vinifera L.)." Genes 10, no. 9 (September 5, 2019): 680. http://dx.doi.org/10.3390/genes10090680.

Повний текст джерела
Анотація:
Gibberellin (GAs) plays the important role in the regulation of grape developmental and growth processes. The bioinformatics analysis confirmed the differential expression of GA2, GA3, and GA20 gibberellin oxidase genes (VvGA2oxs, VvGA3oxs, and VvGA20oxs) in the grape genome, and laid a theoretical basis for exploring its role in grape. Based on the Arabidopsis GA2oxs, GA3oxs, and GA20oxs genes already reported, the VvGA2oxs, VvGA3oxs, and VvGA20oxs genes in the grape genome were identified using the BLAST software in the grape genome database. Bioinformatics analysis was performed using software such as DNAMAN v.5.0, Clustalx, MapGene2Chrom, MEME, GSDS v.2.0, ExPASy, DNAsp v.5.0, and MEGA v.7.0. Chip expression profiles were generated using grape Affymetrix GeneChip 16K and Grape eFP Browser gene chip data in PLEXdb. The expression of VvGA2oxs, VvGA3oxs, and VvGA20oxs gene families in stress was examined by qRT-PCR (Quantitative real-time-PCR). There are 24 GAoxs genes identified with the grape genome that can be classified into seven subgroups based on a phylogenetic tree, gene structures, and conserved Motifs in our research. The gene family has higher codon preference, while selectivity is negative selection of codon bias and selective stress was analyzed. The expression profiles indicated that the most of VvGAox genes were highly expressed under different time lengths of ABA (Abscisic Acid) treatment, NaCl, PEG and 5 °C. Tissue expression analysis showed that the expression levels of VvGA2oxs and VvGA20oxs in different tissues at different developmental stages of grapes were relatively higher than that of VvGA3oxs. Last but not least, qRT-PCR (Real-time fluorescent quantitative PCR) was used to determine the relative expression of the GAoxs gene family under the treatment of GA3 (gibberellin 3) and uniconazole, which can find that some VvGA2oxs was upregulated under GA3 treatment. Simultaneously, some VvGA3oxs and VvGA20oxs were upregulated under uniconazole treatment. In a nutshell, the GA2ox gene mainly functions to inactivate biologically active GAs, while GA20ox mainly degrades C20 gibberellins, and GA3ox is mainly composed of biologically active GAs. The comprehensive analysis of the three classes of VvGAoxs would provide a basis for understanding the evolution and function of the VvGAox gene family in a grape plant.
Стилі APA, Harvard, Vancouver, ISO та ін.
3

Phong, Ong Xuân, Lý Khánh Linh, La Việt Hồng, Đỗ Tiến Phát та Phạm Bích Ngọc. "BIẾN NẠP VÀ ĐÁNH GIÁ CÁC DÒNG XOAN TA (Melia azedarach L.) MANG GEN GA20-OXIDASE ĐƯỢC ĐIỀU KHIỂN BỞI PROMOTER GmPrP2". TNU Journal of Science and Technology 227, № 14 (15 вересня 2022): 113–20. http://dx.doi.org/10.34238/tnu-jst.6356.

Повний текст джерела
Анотація:
Cây xoan ta là một cây lâm nghiệp có nhiều công dụng, ở Việt Nam cây xoan được trồng khá phổ biến. Trong nghiên cứu này cấu trúc mang gen GA20ox mã hoá cho enzyme GA20-oxidase đóng vai trò quan trọng trong quá trình sinh tổng hợp GA ở tế bào thực vật đã được chuyển vào cây xoan ta bằng vector GmPrP2:GA20ox. Kết quả kiểm tra sự có mặt của gen GA20ox bằng kỹ thuật PCR có 15 dòng mang gen. Các dòng xoan mang gen chuyển được đánh giá mức độ biểu hiện ở cấp độ phân tử bằng kỹ thuật RT-PCR cho kết quả gen GA20ox đã hoạt động. Đánh giá sinh trưởng các dòng mang gen chuyển sau 8 tuần tuổi thì dòng T16, T20 và T22 có chiều cao và số đốt tăng 150 - 200% so với đối chứng. Giải phẫu đốt thân thứ 8 cho thấy mật độ sợi xylem và số tế bào trên sợi xylem của các dòng mang gen chuyển đều cao hơn dòng đối chứng, còn độ dày thành tế bào các dòng tương tự nhau. Những kết quả này bước đầu chứng minh vai trò của promoter GmPrP2 với gen GA20ox đối với quá trình tăng trưởng và cải thiện sinh khối ở cây thân gỗ.
Стилі APA, Harvard, Vancouver, ISO та ін.
4

Ma, Jikai, and Huogen Li. "The Formation of Shapes: Interplay of Genes during Leaf Development Processes." Forests 13, no. 10 (October 20, 2022): 1726. http://dx.doi.org/10.3390/f13101726.

Повний текст джерела
Анотація:
Leaf shape, as one of the clearest manifestations of plant morphology, shows considerable variation owing to genetics and the environment. Leaf initiation occurs in the peripheral zone of the SAM and goes through the three overlapping phases of leaf primordium initiation, leaf dorsiventral development, and leaf marginal meristem establishment. Transcription factors, such as KNOX, WOX, and CUC; hormone-regulating genes, such as GA2ox, GA20ox, and PIN1; and miRNAs such as miR164/165 are tightly involved in leaf shaping through the generation of intricate cooperative networks in different temporal phases and specific tissue zones. Here, we briefly discuss the critical interplay occurring between certain genes and the pivotal role these play in the leaf developmental network and phytohormone regulation, including AS1/AS2–KNOX–GA20ox–GA, miR164–NAM/CUC–PIN1–auxin, and CUC–BAS1/CYP734A–BR, and we attempt to summarize several basic insights into the mechanisms of leaf shape regulation.
Стилі APA, Harvard, Vancouver, ISO та ін.
5

Sun, Xiaorong, Jinshuai Shu, Ali Mohamed Ali Mohamed, Xuebin Deng, Xiaona Zhi, Jinrui Bai, Yanan Cui, et al. "Identification and Characterization of EI (Elongated Internode) Gene in Tomato (Solanum lycopersicum)." International Journal of Molecular Sciences 20, no. 9 (May 5, 2019): 2204. http://dx.doi.org/10.3390/ijms20092204.

Повний текст джерела
Анотація:
Internode length is an important agronomic trait affecting plant architecture and crop yield. However, few genes for internode elongation have been identified in tomato. In this study, we characterized an elongated internode inbred line P502, which is a natural mutant of the tomato cultivar 05T606. The mutant P502 exhibits longer internode and higher bioactive GA concentration compared with wild-type 05T606. Genetic analysis suggested that the elongated internode trait is controlled by quantitative trait loci (QTL). Then, we identified a major QTL on chromosome 2 based on molecular markers and bulked segregant analysis (BSA). The locus was designated as EI (Elongated Internode), which explained 73.6% genetic variance. The EI was further mapped to a 75.8-kb region containing 10 genes in the reference Heinz 1706 genome. One single nucleotide polymorphism (SNP) in the coding region of solyc02g080120.1 was identified, which encodes gibberellin 2-beta-dioxygenase 7 (SlGA2ox7). SlGA2ox7, orthologous to AtGA2ox7 and AtGA2ox8, is involved in the regulation of GA degradation. Overexpression of the wild EI gene in mutant P502 caused a dwarf phenotype with a shortened internode. The difference of EI expression levels was not significant in the P502 and wild-type, but the expression levels of GA biosynthetic genes including CPS, KO, KAO, GA20ox1, GA20ox2, GA20ox4, GA3ox1, GA2ox1, GA2ox2, GA2ox4, and GA2ox5, were upregulated in mutant P502. Our results may provide a better understanding of the genetics underlying the internode elongation and valuable information to improve plant architecture of the tomato.
Стилі APA, Harvard, Vancouver, ISO та ін.
6

Zheng, Feng, Yahan Wang, Dachuan Gu, and Xuncheng Liu. "Histone Deacetylase HDA15 Restrains PHYB-Dependent Seed Germination via Directly Repressing GA20ox1/2 Gene Expression." Cells 11, no. 23 (November 26, 2022): 3788. http://dx.doi.org/10.3390/cells11233788.

Повний текст джерела
Анотація:
Seed germination is essential for the colonization of the land plants. Light is a major environmental factor affecting seed germination, which is predominantly regulated by photoreceptor phytochrome B (PHYB). PHYB is activated by red light (designated as PHYB-on) whereas it is inactivated by far-red light (referred as PHYB-off). We previously reported that Arabidopsis histone deacetylase HDA15 interacts with phytochrome-interacting factor1 (PIF1) to repress seed germination under PHYB-off conditions. Here, we show that HDA15 plays a negative role in regulating seed germination under PHYB-on conditions. Overexpression of HDA15 in Arabidopsis restrains PHYB-dependent seed germination, while gibberellin (GA) relieves the repressive role of HDA15 under PHYB-off conditions. We further show that HDA15 directly binds to GA20ox1 and GA20ox2, two key GA biosynthesis genes and represses their expression by removal of histone H3 and H4 acetylation. Moreover, the levels of HDA15 transcript and HDA15 protein are up-regulated in the phyB mutant. Collectively, our work proposes that HDA15 acts as a negative regulator of PHYB-dependent seed germination by directly repressing GA20ox1/2 gene expression.
Стилі APA, Harvard, Vancouver, ISO та ін.
7

Ding, Qiangqiang, Feng Wang, Juan Xue, Xinxin Yang, Junmiao Fan, Hong Chen, Yi Li, and Han Wu. "Identification and Expression Analysis of Hormone Biosynthetic and Metabolism Genes in the 2OGD Family for Identifying Genes That May Be Involved in Tomato Fruit Ripening." International Journal of Molecular Sciences 21, no. 15 (July 28, 2020): 5344. http://dx.doi.org/10.3390/ijms21155344.

Повний текст джерела
Анотація:
Phytohormones play important roles in modulating tomato fruit development and ripening. The 2-oxoglutarate-dependent dioxygenase (2OGD) superfamily containing several subfamilies involved in hormone biosynthesis and metabolism. In this study, we aimed to identify hormone biosynthesis and metabolism-related to 2OGD proteins in tomato and explored their roles in fruit development and ripening. We identified nine 2OGD protein subfamilies involved in hormone biosynthesis and metabolism, including the gibberellin (GA) biosynthetic protein families GA20ox and GA3ox, GA degradation protein families C19-GA2ox and C20-GA2ox, ethylene biosynthetic protein family ACO, auxin degradation protein family DAO, jasmonate hydroxylation protein family JOX, salicylic acid degradation protein family DMR6, and strigolactone biosynthetic protein family LBO. These genes were differentially expressed in different tomato organs. The GA degradation gene SlGA2ox2, and the auxin degradation gene SlDAO1, showed significantly increased expression from the mature-green to the breaker stage during tomato fruit ripening, accompanied by decreased endogenous GA and auxin, indicating that SlGA2ox2 and SlDAO1 were responsible for the reduced GA and auxin concentrations. Additionally, exogenous gibberellin 3 (GA3) and indole-3-acetic acid (IAA) treatment of mature-green fruits delayed fruit ripening and increased the expression of SlGA2ox2 and SlDAO1, respectively. Therefore, SlGA2ox2 and SlDAO1 are implicated in the degradation of GAs and auxin during tomato fruit ripening.
Стилі APA, Harvard, Vancouver, ISO та ін.
8

Teshome, Shiferaw, and Mulugeta Kebede. "Analysis of regulatory elements in GA2ox, GA3ox and GA20ox gene families in Arabidopsis thaliana: an important trait." Biotechnology & Biotechnological Equipment 35, no. 1 (January 1, 2021): 1603–12. http://dx.doi.org/10.1080/13102818.2021.1995494.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
9

Ashikari, Motoyuki, Akie Sasaki, Miyako Ueguchi-Tanaka, Hironori Itoh, Asuka Nishimura, Swapan Datta, Kanako Ishiyama, et al. "Loss-of-function of a Rice Gibberellin Biosynthetic Gene, GA20 oxidase (GA20ox-2), Led to the Rice ‘Green Revolution’." Breeding Science 52, no. 2 (2002): 143–50. http://dx.doi.org/10.1270/jsbbs.52.143.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
10

Yue, Jing, Heyu Yang, Shaohui Yang, and Jiehua Wang. "TDIF overexpression in poplars retards internodal elongation and enhances leaf venation through interaction with other phytohormones." Tree Physiology 40, no. 1 (December 19, 2019): 60–72. http://dx.doi.org/10.1093/treephys/tpz126.

Повний текст джерела
Анотація:
Abstract As a member of the CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION-related (CLE) peptide family, tracheary element differentiation inhibitory factor (TDIF) plays crucial roles in vascular meristem maintenance by promoting cell proliferation and inhibiting xylem cell differentiation. In Populus trichocarpa, six TDIF-encoding genes are all expressed in vascular tissues, and in Arabidopsis PtTDIFpro:GUS lines, the expression driven by PtTDIF promoters were predominantly detected in stem vascular bundles, initiating leaves and leaf veins. Although exogenous application of two poplar TDIF peptides did not evidently affect the shoot growth in vitro, overexpression of PtTDIF genes in hybrid poplar severely retarded the internodal elongation by upregulating the expression of GA2ox and GA20ox genes and thus decreasing the level of endogenous gibberellins (GAs), which phenotypic defect could be rescued by exogenously applied GA3. In addition, TDIF overexpression unexpectedly induced a more complex venation pattern in poplar leaves, which was underpinned by the elevated expression of WOX4 and WOX13 genes. Upon TDIF treatment, the DR5:GUS poplar leaves revealed a higher GUS activity and in TDIF-overexpressing leaves, the transcript abundances of several PIN-FORMED (PIN) genes, especially that of PIN1, were increased, which implied an integration of TDIF and auxin in mediating this process. Collectively, data of this work presented novel activities of TDIF involved in internode elongation and leaf vein formation, thus revealing the divergent functions of TDIF in perennial tree species from those in annual herbaceous Arabidopsis.
Стилі APA, Harvard, Vancouver, ISO та ін.
Більше джерел

Дисертації з теми "GA200X"

1

大島, 孝仁. "酸化ガリウム系半導体の機能とデバイス応用に関する研究". 京都大学 (Kyoto University), 2010. http://hdl.handle.net/2433/120869.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
2

Ramiz, Zarka. "Genetic Control of Seed Dormancy in Lolium rigidum Gaudin and Bromus diandrus Roth." Thesis, 2022. https://hdl.handle.net/2440/136519.

Повний текст джерела
Анотація:
Bromus diandrus and Lolium rigidum are winter annual grass weeds that have become serious weeds of cereal crops in Australia. Surveys of Australian grain growers over the last 20 years have consistently shown L. rigidum to be one of the most troublesome weeds. Characteristics such as staggered seed germination, herbicide resistance and adaptation to environmental stresses have also made B. diandrus an increasing problem of crop production systems in Australia. From recent studies, some evidence has emerged of changes in germination behaviour of these weeds species that may be making their management more difficult. Studies of genetic variation in seed dormancy within populations of these weeds may help to better understand adaptive response of these weed species to changes in management practices. Even though several researchers have highlighted the need to understand genetic control of weed seed dormancy, not many researchers have investigated this topic. Seed dormancy was assessed in five B. diandrus populations collected from wheat fields in cropping areas of South Australia in 2017. Seedling emergence pattern showed large differences in seed dormancy among these populations. Time to 50% seedling emergence (t50) for B. diandrus populations ranged from 10.3 to 28.5d. Seedling emergence in these populations of B. diandrus continued for up to 80d which provided an opportunity to select early and late emerging cohorts within each population in 2018 and 2019. Evaluation of seeds of the progeny of these cohorts in 2019 and 2020 consistently showed significant differences in seed dormancy. Seeds produced by early cohorts had a lower dormancy than seeds of late cohorts. For example, the seeds of the early cohort of Riverton population had t50 of 23.5d as compared to 46d for the late cohort. A similar pattern of differences in t50 between the seeds of early and late cohorts was also observed in Mallala population. Consistent differences in seed dormancy expression between the early and late cohorts of the same population clearly indicated that the expression of seed dormancy in these cohorts was under strong genetic control. In order to identify genes associated with differences in seed dormancy, seeds of early and late cohorts of Riverton and Mallala were analysed for the expression of ABA1 and GA20ox genes using qPCR at 30, 60, 90 and 120 days after maturity. Higher ABA1 and lower GA20ox gene expression was observed in seeds of late cohorts (high dormancy) compared to the early emerging cohorts (low dormancy). During the after-ripening period, the expression of GA20ox increased and ABA1 decreased, which was correlated with the loss of seed dormancy in late emerging cohorts. These studies have shown large differences in seed dormancy between individuals present within the same populations of B. diandrus. Presence of genetic variation for this important trait could play an important role in their adaptation to escape pre-sowing weed control tactics and become an even greater problem in field crops grown in this region. Similar studies of seed dormancy were also undertaken on L. rigidum. Seeds of seven L. rigidum populations were collected in 2017 from wheat fields on three commercial farms in the medium (Paskeville and Roseworthy) and high (Hilltown) rainfall areas of South Australia. Presence of greater than two-fold differences in seed dormancy between populations from the same farm indicates likely influence of management practices on selection for seed dormancy. For example, the time taken to reach 50% (t50) of total seasonal seedling emergence for 3 populations from a single farm at Roseworthy ranged from 11d to 27d. Similarly, t50 for 2 populations from a farm at Hilltown varied from 13d for HT1 to 23d for HT2. Occurrence of seedling emergence over a long period in L. rigidum populations provided an opportunity to select low (LD) and high dormancy (HD) cohorts. Seeds produced by these cohorts of three L. rigidum populations over two years showed significant differences in seed dormancy between them. Seeds of low and high dormancy cohorts of HT2, PSK1 and RAC3 were concurrently analysed for seed dormancy and the expression of ABA1 and GA20ox using qPCR at 30, 60 and 90 days after maturity. Greater ABA1 and lower GA20ox gene expression was observed in seeds of high dormancy cohorts than in low dormancy cohorts. During the after-ripening period, the expression of GA20ox increased and ABA1 decreased, which was significantly correlated with seed dormancy. The results from this investigation provide evidence for genetic control of seed dormancy in field populations of L. rigidum. Presence of heritable genetic variation in seed dormancy in L. rigidum populations will allow this weed to rapidly adapt to changes in weed management practices such as delayed sowing of crops. As water stress during spring time can be a frequent occurrence in Australian grain growing regions, studies were undertaken to determine the impact of moisture stress during reproductive development on seed dormancy in B. diandrus and L. rigidum populations. Seeds of these populations were also investigated for the expression of genes involved with GA and ABA synthesis. Seeds collected from plants of both species exposed to water stress from GS31 onwards were the most dormant in both weed species. In contrast, plants that were well-watered (control) produced seeds with the lowest dormancy. The seeds collected from the well-watered treatment of both L. rigidum and B. diandrus populations reached 50% germination at 90 days after maturity (DAM) while the germination of stress at GS31 was approximately 50-67% lower than the well-watered (control) treatment. Water stress treatments altered the expression of ABA1 and GA20ox genes which was correlated with the level of dormancy in seeds of B. diandrus and L. rigidum. The ABA1 expression levels were high at 30DAM but decreased during the after-ripening periods. This coincided with the gene expression levels of GA20ox, which were low soon after maturity but gradually increased. The 2-fold increase in the GA20ox levels in all the populations and stress treatments at 90-150DAM points towards the loss of seed dormancy. The differences observed in the seed germination behaviour of water stressed vs non stressed treatments and also the gene expression levels of ABA1 and GA20ox highlighted the fact that effects of water stress on seed dormancy are mediated through these dormancy regulating genes in these two weed species.
Thesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2022
Стилі APA, Harvard, Vancouver, ISO та ін.
3

CHIU, CHIEN-CHIH, and 邱建志. "Regulation of Gibberellin by GA2ox Gene in Petunia (Petunia x Hybrida ’Mitchell Diploid’)." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/du5u4s.

Повний текст джерела
Анотація:
碩士
元培醫事科技大學
生物科技暨製藥技術系碩士班
107
In this study, the oxidative function of Arabidopsis thaliana AtGA2ox8 was genetically engineered into Petunia x hybrid ‘Mitchell Diploid’ to alter GA (Gibberellin) biosynthesis. The purpose is to reduce the bioactive GA levels in plant to achieve dwarfism. In traditional horticulture practice, many physical or chemical methods are employed to control the height of plant. They are always labor-consuming with low efficiency. Spraying chemicals is one of the fastest solution. However, this is not quite environmental friendly. In order to overcome this issue, genetic engineering is an alternative option. GA belongs to plant hormone that can regulate the height during plant growth and development. By transforming a GA oxidase gene to convert bioactive GA’s into inactive forms or other structures in plants resulting in dwarf phenotype. In this project, petunias (Mitchell Diploid; MD) were previously transformed with coding region of AtGA2ox8 under the control of CaMV 35S promoter. The At2GAox8 transgenic lines were confirmed by PCR analysis. Three transgenic lines with dwarf phenotype were selected and designated as D8, D12, D15, and the wild type MD as control. The plant heights from the lowest to the highest were D8 (13.60±3.48 cm), D15 (27.55±3.82 cm), and D12 (60.75±2.66 cm) after 22 weeks of planting. The plant heights of D8 and D15 were significantly shorter than those of MD (64.20±3.48 cm). Branch numbers at 22 weeks of planting were 8.10±2.47 (D8) and 9.10±2.47 (D15) that were significantly different from 17.70±2.53 (MD). The growth of root systems were not affected significantly among transgenic lines and MD. Gene expression levels of At2GAox8 in roots, stems, leaves and petals from transgenic plants D8, D15, D12, and wild type MD were analyzed by RT-PCR assay. The results demonstrated that the At2GAox8 expression in D12 was less than the other transgenic plants in the aerial parts and petals of plant. Our results confirmed that transgenic At2GAox8 gene can oxidize GA’s in petunia to alter whole plant structure and achieve dwarfism. The applications of AtGA2ox8 gene in ornamental plants are possible, and this plant biotechnology not only can be further utilized in horticulture to reduce the applications of chemicals but also adding the ornamental value for plants.
Стилі APA, Harvard, Vancouver, ISO та ін.
4

Pai, Yu-Min, and 白育旻. "Searching for transcription factors involved in regulating expression of GA2ox genes and characterization of a high tiller number T-DNA insertion mutant M69217." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/16729582236393637615.

Повний текст джерела
Анотація:
碩士
國立中興大學
分子生物學研究所
103
GA (gibberellic acids) is one of the plant hormones involved in plants development, excessive GA cause plant lodging and lack of GA cause plant dwarf. GA2-oxidases are enzymes catalyze bioactive GA or GA precursors to inactive GAs and to regulate GA homeostasis in plants. There are nine GA2-oxidase genes in rice (OsGA2ox1–9) and how these gene expressions are regulated temporally and spatially in rice plant have not yet been characterized. This study intended to search for transcription factors involved in regulating the expression of OsGA2ox genes. After bioinformatics analysis, promoter sequences of most OsGA2ox genes contain DOF (DNA binding with one finger) transcription factor binding site. Phylogenetic analysis of 33 OsDOFs and their possible involvement in GA regulation based on previous studies, totally 7 T-DNA insertion mutants, including OsDOFACT (M51680 and M53794)、OsDOF7ACT (M63981)、OsDOF10ACT (M45005)、OsDOF10KNO (M78151)、OsDOF24KNO (M61519) and OsDOF25ACT (M66458), from TRIM library were collected. After field observation, mutant M53794 (for OsDOF1 target gene) and mutant M61519 (for OsDOF24 target gene) revealed slightly dwarf phenotype and were further characterized. The RNA expression of two target genes, OsDOF1 and OsDOF24, were analyzed by RT-PCR. Gene of OsDOF1 showed increased expression in M53794 and DOF24 in M61519 showed the same expression level as those of TNG67. Expression of OsGA2ox genes in these two mutants were analyzed and neither significant nor consistent levels of expression for OsGA2ox genes was observed between mutant and TNG67, suggested that the expression of OsGA2ox genes were not regulated by OsDOF1 or OsDOF24 and the inconsistent expression of OsGA2ox genes might cause by the environment factors. Mutant M69217 showed severe dwarf and high tiller number and its flanking genes CM (Chorismate mutase) and CCR (Cinnamoyl-CoA reductase) were activated. However overexpression of CM and/or CCR genes could not recapitulate the phenotype of M69217. In this study, further characterization of this mutant and identification of genes responsible for its phenotype was investigated. Further analysis showed another gene, GNAT (GCN5-related N-acetyltransferase), located near the T-DNA insertion site was also activated. Overexpressing GNAT in transgenic rice to investigate its effect was underway. Another T-DNA insertion mutant M111350 revealed the same phenotype as that of M69217 and results suggested that the unstoppable tillering might cause by activation of miR156b. It was interesting to realize that another miR156d gene was located near the insertion site as well. However, the expression of miR156d in M69217 was no significant different from that in TNG67, but the miR156d target gene SPL16 (SQUAMOSA Promoter-Binding-Like Transcription Factors) decreased in M69217. Overexpressing miR156d in transgenic rice was performed to investigate its effect on tillering.
Стилі APA, Harvard, Vancouver, ISO та ін.

Книги з теми "GA200X"

1

Notebook, charlesvineyard. Composition Notebook: Lemur Monkey Madagascar Jungle Aye Aye Zoo Ga20 - 50 Sheets, 100 Pages - 6 X 9 Inches. Independently Published, 2020.

Знайти повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.

Частини книг з теми "GA200X"

1

Xu, Y., R. Grote, Y. Wen, L. Shuller-Nckles, and K. S. Brinkman. "Development of Ga Doped Hollandites Bax Csy (Ga2x+y Ti8-2x-y )O6 for Cs Immobilization." In Ceramic Transactions Series, 157–64. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2016. http://dx.doi.org/10.1002/9781119234531.ch14.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
2

"GA20 The Dead Adonis, by Robert Cholmeley (Yale MS Osborn c190)." In Classical Presences: Newly Recovered English Classical Translations, 1600–1800. Oxford University Press, 2018. http://dx.doi.org/10.1093/oseo/instance.00251132.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
3

Satsuma, A., A. Gon-no, K. Nishi, S. Komai, and T. Hattor. "Contributions of three types of Ga sites in propane aromatization over Ga203/Ga-M0R catalysts." In New Developments and Application in Chemical Reaction Engineering, 257–60. Elsevier, 2006. http://dx.doi.org/10.1016/s0167-2991(06)81582-x.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.

Тези доповідей конференцій з теми "GA200X"

1

Putman, Matthew. "AI process improvements for Ga203." In Oxide-based Materials and Devices XIII, edited by Ferechteh H. Teherani and David J. Rogers. SPIE, 2022. http://dx.doi.org/10.1117/12.2631672.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
2

Zeng, Ke, Abhishek Vaidya, and Uttam Singisetti. "710 V Breakdown Voltage in Field Plated Ga203 MOSFET." In 2018 76th Device Research Conference (DRC). IEEE, 2018. http://dx.doi.org/10.1109/drc.2018.8442222.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
3

Apriana, Aniversari, Tri Joko Santoso, Atmitri Sisharmini, Reflinur, A. Dinar Ambarwati, Toto Hadiarto, Sustiprijatno, and Nuryati. "Phenotypic and genetic stability evaluation of the targeted GA20ox-2 gene mutation in CRISPR/Cas9 mutant rice derived from Mentong cultivar." In THE SECOND INTERNATIONAL CONFERENCE ON GENETIC RESOURCES AND BIOTECHNOLOGY: Harnessing Technology for Conservation and Sustainable Use of Genetic Resources for Food and Agriculture. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0075603.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
4

"ORIENTATsIONNAYa ZAVISIMOST'' ELASTOKALORIChESKOGO EFFEKTA V MONOKRISTALLAKh SPLAVA Ni50Mn30 Ga20." In Fizicheskaya mezomekhanika. Materialy s mnogourovnevoy ierarkhicheski organizovannoy strukturoy i intellektual'nye proizvodstvennye tekhnologii. Tomsk State University, 2020. http://dx.doi.org/10.17223/9785946219242/187.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
5

Nicolini, Valeria, Sylvia Herter, Sabine Lang, Inja Walhauer, Erwin van Puijenbroek, Claire Dunn, Pablo Umana, and Christian A. Gerdes. "Abstract A156: GA201 (RG7160) pretreatments and combination therapies improve efficacy without negatively affecting antitumoral ADCC." In Abstracts: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics--Nov 12-16, 2011; San Francisco, CA. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1535-7163.targ-11-a156.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
6

Choi, Yong Gyu, Kyong-Hon Kim, Vladimir A. Chernov, and Jong Heo. "X-ray absorption spectroscopic analyses and fluorescence emission characteristics of PbO-Bi203-Ga203 glasses doped with rare-earth ions." In Photonics East '99, edited by Mohammed Saad and James A. Harrington. SPIE, 1999. http://dx.doi.org/10.1117/12.372801.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
7

Pool, Martin, Arjan Kol, Marjolijn N. Lub-De Hooge, Christian A. Gerdes, Steven de Jong, Elisabeth G. E. de Vries, and Anton G. T. Terwisscha van Scheltinga. "Abstract 4931: ImmunoPET and fluorescence imaging with Zirconium-89 and IRDye 800CW labeled glycoengineered epidermal growth factor receptor antibody GA201." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-4931.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
8

Kol, Arjan, Steven de Jong, Martin Pool, Elisabeth G. E. de Vries, Christian A. Gerdes, and Anton G. T. Terwisscha van Scheltinga. "Abstract 4511: Differential effects of GA201 and cetuximab on EGFR expression and endosomal recycling in non-small cell lung cancer cell lines." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-4511.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
9

Oppenheim, David, David Malone, Aniekan Etuk, Timothy Murray, Laura McLaughlin, Roberto Spreafico, Claudia Pena-Murillo, et al. "Abstract C221: Glycoengineered anti-EGFR (GA201) elicits enhanced ADCC responses by NK cells from colorectal cancer patients despite tumor-associated impairments to natural cytotoxicity." In Abstracts: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics--Nov 12-16, 2011; San Francisco, CA. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1535-7163.targ-11-c221.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
10

Xu, Bei, Jichao Hu, Xiaomin He, Xi Wang, Dan li та Chunlan Chen. "Effect of Growth Temperature on the Characteristics of β-Ga203 Thin Films Grown on 4H-SiC (0001) Substrates by Low Pressure Chemical Vapor Deposition". У 2021 IEEE 4th International Conference on Electronics Technology (ICET). IEEE, 2021. http://dx.doi.org/10.1109/icet51757.2021.9450967.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
Також вас можуть зацікавити розширені добірки на тему "GA200X" для конкретних типів джерел:
Статті в журналах
Ми пропонуємо знижки на всі преміум-плани для авторів, чиї праці увійшли до тематичних добірок літератури. Зв'яжіться з нами, щоб отримати унікальний промокод!

До бібліографії